CN114467864A - Method for controlling species group of Exorista japonica - Google Patents

Method for controlling species group of Exorista japonica Download PDF

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CN114467864A
CN114467864A CN202210082504.7A CN202210082504A CN114467864A CN 114467864 A CN114467864 A CN 114467864A CN 202210082504 A CN202210082504 A CN 202210082504A CN 114467864 A CN114467864 A CN 114467864A
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silkworm
population
silkworms
controlling
fly larvae
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CN114467864B (en
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卫静
李兵
代敏丽
蒋学建
李凡池
朱清宇
顾欣然
孙海娜
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Suzhou University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • A01K67/04Silkworms
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention discloses a method for controlling the population of the Japanese migratory fly, the Japanese migratory fly is a natural enemy insect for biological control, can parasitize more than 30 lepidoptera pests such as fall webworms, pine moths, armyworms, pieris rapae, corn borers and the like and is widely applied, and the Japanese migratory fly can parasitize larvae of the silkworm as an economic insect after being released in the nature, so that improper population control can influence the silkworm production. The method for controlling the population of the rice fly larvae disclosed by the invention is characterized in that the silkworm variety of five instars, namely the silkworm variety of pinus sylvestris and aphelia rosepalae, is bred and developed in the silkworm area with the rice fly larvae as the pests and exposed in the open environment to attract the parasitism of the rice fly larvae, creatively discovers that the rice fly larvae are preferred to parasitize the rice fly larvae, can obviously reduce the population quantity of the rice fly larvae in the silkworm area, particularly the parasitized silkworms can normally cocoon, does not influence the quality of the yield of silkworm cocoons, reduces the harm of the rice fly larvae to the production of silkworms, and has wide application prospects.

Description

Method for controlling species group of Exorista japonica
Technical Field
The invention belongs to the field of biological control, and particularly relates to a method for controlling a Chrysomyia macranthoides population.
Background
Gypsophila japonica (Misch.) KuckExorista japonica(Townsend) (Diptera parasitidae) is a large-egg type parasitic fly, which is laid on the body surface of a host by female flies, hatched and drilled into the body cavity of the host, and finally drilled out to pupate the host at the host larva or pupate stage; it can be used for parasitizing various agroforestry pests, including 30 lepidoptera pests such as fall webworm, pine moth, armyworm, pieris rapae, corn borer, etc. After the flies from the species of the domestic silkworms are released into the natural world, the species of the flies from the species of the domestic silkworms are difficult to control under natural conditions, and the flies from the species of the domestic silkworms parasitized and endangered by the flies from the species of the domestic silkworms (Bombyx mori, and the like are found in recent yearsBombyx moriL.) larvae. In the prior art, a physical method and a medicinal method are generally adopted to avoid parasitizing the domestic silkworms by virtue of the parasitic flies, for example, silkworm flies are killed or the net is pulled, but the medicaments harm the domestic silkworms, so that the silkworms die, and the fly prevention device mainly aims at a silkworm rearing room, has poor effect and is not suitable for greenhouse cultivation. Therefore, there is a strong need for biological control to develop a method for controlling the population of natural enemies released, and in addition, there is a need for controlling the population of the flying flies, namely, the flying flies, in the silkworm breeding area, so that the balanced development of biological control and silkworm industry safety production can be realized under the level of economic harm.
Disclosure of Invention
At present, silkworm flies are killed in silkworm breeding production as main medicines for preventing and controlling the fly catching flies of silkworms, but the long-term use of the fly catching flies of silkworms causes drug resistance, and the prevention and control effect is obviously reduced, so that the loss caused by the damage of the fly catching flies of silkworms is more and more serious, and the silkworm breeding production is influenced. The invention aims to provide a method for controlling the Japan bactrocera dorsalis population, which solves the problem that chemical drugs or physical measures are needed for killing flies in the prior art.
In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows:
a method for controlling the population of the rice fly postlarvae is characterized in that the silkworm is exposed to the natural environment to attract the rice fly postlarvae so as to realize the control of the population of the rice fly postlarvae, and preferably, the variety of the silkworm is pinus sylvestris x Haoyue.
A method for controlling the population of the Chrysomyia Zebra flies during silkworm breeding production comprises the steps of breeding the Chrysomyia Zebra X Haoyue silkworm larvae to 5 instars, putting the 5 instars Chrysomyia Zebra X Haoyue silkworm in a natural environment outside a silkworm breeding area, exposing the silkworm larvae to the natural environment, attracting the Chrysomyia Zebra flies to parasitize, and realizing the control of the population of the Chrysomyia Zebra flies. Preferably, the quantity of throwing in 5 age chinese cyanine pine x gospel domestic silkworms is 1000 ~ 10000 heads, in the natural environment outside the sericulture region, 5 age 1 day chinese cyanine pine x gospel domestic silkworms are thrown in subregion.
The invention discloses an application of a bombyx mori in controlling a trichomonas mobilis population, in particular to an application of a bombyx mori in controlling a trichomonas mobilis population during silkworm breeding production, and preferably an application of a bombyx mori in reducing the parasitic rate of a conventional bombyx mori by the trichomonas mobilis during silkworm breeding production.
In the invention, the silkworms with the cyanine pine and the Haoyue are first-generation hybrid species, so that the influence of pupation and oviposition caused by parasitism of the stock species is avoided; the silkworm variety selected by the method is pinus sylvestris x Haoyue, and has obvious advantages in controlling the species of the Exorista civilis Rond, which is provided for the first time by the invention.
According to the method, the selected silkworm variety is Cyanine pine multiplied by Haoyue, and is raised indoors to 5-year-old silkworm, so that the phenomenon that 4-year-old larva is parasitized and then desquamate abnormally in the sleep period to cause death is avoided; the exposure to the natural environment is 20-25 ℃, and the light is directly emitted. The feed which is favored by the Japan migratory flies is adopted to investigate the number of the migratory fly populations in the environment, the feed is mainly prepared by mixing honey, essence and the like, and the application effect of the method is evaluated. Statistics show that the method can reduce the bactrocera dorsalis population in the environment by more than 80%.
Due to the application of the technical scheme, compared with the prior art, the invention has the following advantages:
1. the invention provides a method for controlling the population of the Japanese parasitoid fly, the selected silkworm variety has obvious parasitosis preference of the Japanese parasitoid fly, and the first generation hybrid produced by the silkworm can also form cocoons without influencing the economic value.
The method for controlling the Japan house fly catch flies is an environment-friendly, green and safe biological control method, has the characteristics of low cost, convenient operation, economy and practicality, and is worth popularizing and applying in silkworm areas.
Detailed Description
Aiming at the problem that no effective method for controlling the Japan bactrocera dorsalis population and the harm of the Japan bactrocera dorsalis on the silkworm production exists at present, the invention discloses a novel method for controlling the Japan bactrocera dorsalis population on the production by using silkworms for the first time, and the specific embodiment of the invention is further described in detail below.
Experimental materials: silkworm variety: the varieties of Jingsong is multiplied by Haoyue, Qifeng is multiplied by white jade, Suhao is multiplied by bright day, and the like, and the varieties have the common characteristics of the common varieties of the silkworms. The species of: suzhou university mulberry research institute resource. Adult feed for the bactrocera dorsalis: a mixture of honey and flavours. Silkworm breeding is a conventional method. The body surface of the silkworm has flying flies which are similar to white sesame grains in size and shape and are easy to distinguish.
Example a method for controlling the population of Chrysomya Zebra in an environment
Before silkworms are produced in 5 months in 2020, four silkworm breeding areas with the square meters of 1000 are respectively selected in an east Chenzhen town, a warp carrying town and a grinding head town of Rugao city in Jiangsu province at intervals, and the soldiers in the respective self-breeding silkworm areas do not influence each other; feeding conventional silkworm varieties (greenhouse breeding, Suhao times bright day) in the control area; the test areas are exposed and raised to 5-instar cypress multiplied by Haoyue larvae outdoors on the basis of the control areas, and 1000 heads are respectively thrown in the east, south, west, north corners and middle of each test area to attract parasitic flies catching on the market; the contrast area is exposed and raised to 5-year-old Qiufeng multiplied white jade larvae outdoors on the basis of the contrast area, and 1000 larvae are respectively thrown in the east, south, west, north corners and middle of each test area; bait zone on the basis of the control zone, the adult flies of the type of the fly were exposed to the outside for placing: the honey and essence mixture was placed evenly in the east, south, west, north and middle of each test area. After the cultivation is finished, adult flies of the parasitic flies are adopted to lure the Japanese parasitic flies in the environment, the population quantity is counted, and the change situation of the population quantity of the Japanese parasitic flies before and after the method is used is shown in the table 1.
Figure 430264DEST_PATH_IMAGE001
Table 1 the results show that: the average population number of the Exorista civilis Rond in the control area is 193 heads/1000 square meters, the population number of the Exorista civilis Rond is reduced by 86 percent by using the method, so that the population number of the Exorista civilis Rond in the silkworm area can be effectively controlled by using the method, and the Apriona pinicola x Haoyue can be used for conventionally picking cocoons without influencing the cocooning rate, and on the other hand, the Exorista civilis cannot break the cocoons to cause death. In other breeding areas, measures for killing the silkworm flies are adopted, and in contrast, the method for killing the silkworm flies has no good effect of controlling the number of the fly catching populations, and the silkworm flies are killed.
Figure 816246DEST_PATH_IMAGE002
Table 2 the results show: the parasitic rate of the parasitic flies is higher and reaches 40% in the cultivated silkworm area without exposing and breeding the cultivated silkworms in the Pinus sylvestris times Haoyue outdoors; the outdoor exposure is raised in the cultivated silkworm area of pinus sylvestris x Haoyue, and the parasitic rate of the parasitic flies is lower, only 2%. Therefore, the method can effectively protect the bred silkworms and reduce the parasitic rate of the bactrocera dorsalis.
Example two influence of parasitic on Exorista nipponica on silkworm cocoon quality
Silkworm (Pinus sikokiana x Haoyue) is raised to 5 instars, and is placed in a cage for breeding the parasitic bombyx mori, 1000 bombyx mori larvae are selected to be bred to the upper cocooning, and no parasitosis of the parasitic bombyx mori exists in a control area. And (4) surveying 50 male and female cocoon particles, taking an average value, and counting the cocoon forming rate, the whole cocoon weight, the cocoon shell weight and the cocoon shell rate. Statistics shows that the parasitized individuals and the parasitized individuals can normally cocoon, the cocoon forming rate reaches 99%, and no obvious difference exists. There was no significant difference between the total cocoon mass and the cocoon layer rate, indicating that the use of 5-instar silkworm larvae for controlling the flies catch flies in japan did not affect the yield and quality of the cocoons, as shown in table 3.
Figure 923879DEST_PATH_IMAGE003
After silkworms are parasitized by the parasitic flies before the 5 th instar, the parasitizing rate is high, and after the parasitized silkworm developmental plastid age dormancy stage, a large number of silkworms cannot exuviate and are metamorphosed, so that the silkworms die, and the production harm to the silkworms is great. In the prior art, development of novel medicaments for preventing and treating the domestic silkworm fly hunting flies is mainly used, and besides chemical medicament prevention and treatment, other prevention and treatment methods are also applied, such as physical prevention and treatment, a luminous attractant with an attracting and killing function is arranged for prevention and treatment by utilizing phototaxis of the domestic silkworm fly hunting flies and selectivity of different spectrums, or a device with a chemical attracting function is arranged for killing by utilizing sensitivity of the domestic silkworm fly hunting fly adults to certain chemical substances. However, the effect of the existing method is not obvious, and sometimes the method causes harm to silkworm breeding. The method adopts a pure ecological method, creatively provides that the cabbage pine and Haoyue silkworms are used for controlling the parasitic fly trapping population, greatly reduces the number of the parasitic fly trapping population in the silkworm breeding area, has no harm to the bred silkworms, and improves the breeding result of high-value silkworms.

Claims (10)

1. A method for controlling the population of the Chrysomyia Zebra, which is characterized in that the method comprises the steps of exposing silkworms to natural environment to attract the Chrysomyia Zebra parasitism and realize the control of the Chrysomyia Zebra population.
2. The method for controlling the population of Exorista nipponensis according to claim 1, wherein the variety of Bombyx mori is Pinus sylvestris x Haoyue.
3. The method for controlling the population of the rice fly larvae of the cotton fly larvae of the silkworms of the kola silkworms of claim 1, wherein the rice fly larvae are raised to 5 th instar and then exposed to the natural environment to attract the rice fly larvae of the cotton fly larvae of the rice.
4. The method for controlling a bactrocera nipponensis population according to claim 1, wherein the bactrocera nipponensis population is controlled for silkworm breeding.
5. A method for controlling the population of the Exorista civilis Rond during silkworm breeding production is characterized in that 5-year-old silkworms with cyanine pine multiplied by Haoyue are thrown in the natural environment outside a silkworm breeding area during silkworm breeding production, so that the control of the population of the Exorista civilis Rond is realized.
6. The method for controlling the population of Exorista civilis when breeding silkworms according to claim 5, wherein the number of 5-year-old Neisseria zibethanensis x Haoyue silkworms is 1000 to 10000.
7. The method for controlling the population of Exorista civilis when breeding silkworms according to claim 5, wherein 5-year-old Nephelus x Haoyue silkworms are thrown in different areas in the natural environment outside the silkworm breeding area.
8. Application of Cyanine pine and Haoyue silkworm in controlling the species group of the Sciadoptera japonica.
9. The application of the bombyx batrachyces silkworms in controlling the species group of the Exorista civilis Rond during silkworm breeding production.
10. The application of the Cyanine pine and Haoyue silkworm in reducing the parasitism rate of the conventional silkworm by the Exorista japonica during silkworm breeding and production.
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Citations (4)

* Cited by examiner, † Cited by third party
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Patent Citations (4)

* Cited by examiner, † Cited by third party
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JP2004166652A (en) * 2002-11-22 2004-06-17 National Institute Of Agrobiological Sciences Feed including chitin synthesis inhibitor capable of increasing infection rate of recombinant virus to insect and method of seeding recombinant virus by using the same
CN101427667A (en) * 2008-10-31 2009-05-13 江苏科技大学 Breeding method for cultivated silkworm breed variety of fluorine resistant larva and non-palea perfect insect wing
US20200205369A1 (en) * 2017-07-25 2020-07-02 Proti-Farm R& D B.V. Method and system for controlling the climate of an insect rearing climate area
CN111264473A (en) * 2020-02-18 2020-06-12 广西壮族自治区林业科学研究院 Method for breeding Exorista bigelovii by utilizing galleria mellonella

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