CN1144614A - Biological active animal albumen powder - Google Patents
Biological active animal albumen powder Download PDFInfo
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- CN1144614A CN1144614A CN95106362A CN95106362A CN1144614A CN 1144614 A CN1144614 A CN 1144614A CN 95106362 A CN95106362 A CN 95106362A CN 95106362 A CN95106362 A CN 95106362A CN 1144614 A CN1144614 A CN 1144614A
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Abstract
A bioactive animal protein powder rich in VB and balanced amino acids is prepared from imported beneficial bacterial line EM-1, specially selected domestic three beneficial bacteria, two saccharomycetes, and one mould as strains, acid-pretreated dechromized leather and hide powders, and fresh animal blood as base materials, and complemented with cottonseed and rapeseed cakes, corn protein powder, feather powder, wheat bran, corn starch and ammonium sulfate through solid fermentation, baking, crushing and packaging, and serve to promote growing of animals and prevent and cure intestinal infectious diseases. Its bacterial contents are 4 billions/g for beneficial bacteria, (billion/g for saccharomycetes and 0.8 billions/g for filamentous fungus spores.
Description
(1) biological active animal albumen powder belongs to protein fodder science and technology and using microbe technical field.
(2) prior art and deficiency thereof
Along with the develop rapidly of aquaculture, the contradiction of high protein inadequate resource becomes increasingly conspicuous.Actively seeking and developing protein feed resources for addressing this problem over past ten years both at home and abroad field of fodder.Feed protein resource that has been developed to such as leather waste (blue skin, rawhide), animal blood, discarded internal organ, hair and its protein content of bone are higher, and various amino acid balances are better.Present treatment process comprises: 1. fermented blood meal and nonfermented blood meal (forming by the drying process convection drying); 2. hydrolysis leather powder; 3. take off chromium (Cr) leather protein powder; 4. leather powder etc. ferments.
Through this class animal albumen powder that non-zymotechnique is handled, its proteinic digestibility is all lower, and generally at 40-60%, and palatability is not good.And at present through the product of zymotechnique production owing to do not screen fermentation strain preferably as yet, so protein rates of decomposing and transforming is lower during the fermentation, be no more than 15%.
(3) goal of the invention:
The present invention is directed to the technical deficiency of existing exploitation feed protein resource both at home and abroad, mainly reach following purpose:
1, the digestibility of tankage such as raising leather powder and animal blood;
2, make to contain a large amount of probiotic bacteriums in the fermented product, be beneficial to growing and the control of alimentary infection disease of animal;
3, improve palatability;
4, make in the product amino acid whose composition more reasonable by fermentation.
(4) core content of the present invention:
1, this product is to select for use 92 elite strain bacterial classifications as fermented bacterium, comprising:
(1) draws from Japanese EM-1 fungus strain, form by 86 probiotics;
The domestic probiotic bacteria of (2) three strains:
Bifidus bacillus (Bifidobacterium sp.),
Bacillus cereus (Bacillus cereus),
Bacillus licheniformis (Bacillus lichenis);
(3) two saccharomycetes:
Endomycopsis Fibnligera (Endomycopsis fibuligera),
Candida tropicalis (Candida tropicalis);
(4) one strain moulds:
Neuraspora crassa (Neurospora crassa).
2, with above-mentioned 92 strain fermented bacteriums in quadrature ratio combination, then to so that to take off chromium (Cr) Lan Pipi powder, rawhide skin powder and animal blood be base-material and be aided with raw material that cottonseed cake, rapeseed cake, Zein powder, wheat bran, feather meal, Semen Maydis powder and ammonium sulfate (inorganic salt) etc. are formed and carry out consubstantiality and ferment.
3, be 2 hydrochloric acid soln pre-treatment two hours with leather protein powder (being Lan Pipi powder and rawhide skin powder) with PH before the fermentation.The concrete practice was at room temperature stacked two hours in 1: 1 ratio the technical hydrochloric acid of thousandth concentration is admixed in the leather protein powder, carried out the consubstantiality fermentation together with other raw materials again.Pretreated result can obviously improve the fermentation appearance of leather protein powder, makes its unit weight cell count than increasing more than three times without pretreated leather powder.
(5) the concrete implementation step of the present invention
1, bacterial classification is made:
(1) EM-1 fungus strain (86 strain): provide second class inoculum (liquid) by the joint Jiangsu Province's EM-1 strain plant that builds up of Japanese Ryukyu university and cultural exchanges with foreign countries center, Jiangsu Province.Carrying out three, four two-stages then cultivates.
1. cultivate flow process:
Commercially available EM-1 second class inoculum (liquid) → three-class strain (fluid enlargement culture) → level Four bacterial classification (solid culture) → cryodrying (below 80 ℃) → pulverizing.
2. three-class strain is made:
With potato (10%), wheat bran (2～20%), water (85～88%) is substratum (liquid state), liquor; The liquor after-filtration; The filtrate autoclaving; Connect second class inoculum, leave standstill cultivation.
3. the level Four bacterial classification is made:
With dregs of beans (60%), fish meal (10%), corn (20%), wheat bran (10%) is substratum; With raw material pulverizing, be mixed in proportion; Profit water, autoclaving; Connect three-class strain, under aseptic condition, cultivate; Cryodrying; Pulverize.
(2) microbial culture:
Aerobic bacillus cereus and anaerobic bifidobacterium, Bacillus licheniformis are cultivated respectively.
1. cultivate flow process:
Inclined-plane → second class inoculum (liquid culture) → three-class strain (consubstantiality cultivation) → cryodrying (below 80 ℃) → pulverizing.
2. bacterial classifications at different levels are made
A, inclined-plane (solid)
With peptone (0.5%), agar (2%), extractum carnis (0.3%), yeast extract paste (0.3%), NaCl (0.5%), KH
2PO
4(0.3%), MgSO
4(0.1%) be substratum, PH is 7.2; The raw material autoclaving; Inoculation; Cultivate.
B, second class inoculum (liquid)
The same inclined-plane of culture medium prescription does not only add agar, PH7.2; Bottling; Autoclaving; Connect slant strains; Leave standstill cultivation.
C, three-class strain (solid)
With dregs of beans (20～25%), corn (30～40%), wheat bran (10～20%), rice (5～10%), mung bean protein powder (10～20%) is substratum; Raw material is mixed in proportion after crushed; Profit water, autoclaving; Connect second class inoculum, aseptic condition bottom fermentation 24-36 hour; Cryodrying; Pulverize.
(3) yeast culture:
Two saccharomycetes difference is single culture as follows;
A, primary inclined plane is cultivated, and adopts malt extract medium, cultivates 24 hours down for 30 ℃, and is standby.
B, the secondary triangular flask is cultivated, culture medium prescription: Semen Maydis powder (50%), wheat bran (30%), dregs of beans (20%); Material: water=1: 0.7, cultivated 18 hours down at 30 ℃, 60 ℃ of forced air dryings, standby.
C, three grades of enlarged culturing, the same secondary of substratum, inoculum size 5% (inoculation secondary) was cultivated 18 hours down for 30 ℃ in porcelain dish, and 60 ℃ of forced air dryings are standby.
(4) mould is cultivated:
A, primary inclined plane is cultivated, and adopts the potato substratum, 28 ℃ cultivate down 24 hours standby.
B, second class inoculum is cultivated (liquid), and the same slant culture of culture medium prescription was cultivated 18 hours down for 28 ℃, and 60 ℃ of forced air dryings are standby.
C, three grades of enlarged culturing, substratum is the same, and inoculum size 5% was cultivated 20 hours down for 30 ℃ in porcelain dish, and 60 ℃ of forced air dryings are standby.
2, the bacterial classification combination:
Inoculum size 2%, all bacterium ratios are: the EM-1 fungus strain accounts for 50%, and Endomycopsis Fibnligera accounts for 10%, and candida tropicalis accounts for 5%, and coarse arteries and veins spore is mould to be accounted for 5%, three kind of probiotic bacterium (bacterium) and respectively accounts for 10%.
3, the fermentation raw material proportioning:
Through pretreated leather protein powder (by taking off chromium Lan Pipi powder and rawhide skin powder is formed) 50%, fresh animal blood 50% (be converted into skeuomorph is real to account for 10%), cottonseed cake 20%, rapeseed cake 5%, Zein powder 5%, feather meal 5%, Semen Maydis powder 3%, wheat bran 1%, ammonium sulfate 1%.
4, zymotechnique:
Various raw materials are weighed in proportion, and in material: the ratio of water=1: 0.5 adds tap water, and material (animal blood water content is in 75%, and material calculates with its dry matter).After stirring, normal-pressure sterilization 2 hours (or autoclaving 1 hour) inserts mixed bacterial by 2% inoculum size when being cooled to 40 ℃.Take thick-layer to ventilate, but should strictly control ventilation to satisfy the growth conditions of anerobe and aerobic bacteria, the biomass that makes it all to reach maximum is for best.Fermentation time is 24-36 hour.
After the fermentation, adopt the oven dry of drum-type technology, its gas flow temperature is no more than 90 ℃, pulverizes quality inspection, packing afterwards.
(6) the key technical indexes of biological active animal albumen powder and advantage:
1, technical indicator:
(1) outward appearance and Oranoleptic indicator: beige has the fermented product special odor, powdery.
(2) physical and chemical index:
Moisture content≤10%
Crude protein 〉=60%
(3) microbiological indicator:
Total plate count 〉=4,000,000,000/g
Yeast sum 〉=1,000,000,000/g
Filamentous fungus spore sum 〉=0.8 hundred million/g
2, advantage:
(1) probiotic bacterium (referring to three kinds of bacteriums such as 86 strain bacterium and bacillus cereus in the EM-1 fungus strain) number is many, so the product biological activity is very high, can promote animal growth, the control infectious intestinal disease.
(2) the protein digestion specific absorption is greater than 80%, improves about 1 times than fermentation raw material not.
(3) amino acid is formed rationally, Methionin particularly, and methionine(Met) and tryptophane reach respectively more than 2.67%, 0.95% and 0.84%.Than not fermentation raw material raising 20～53%.
(4) be rich in vitamin B group, good palatability.
(5) through increasing more than three times without pretreated leather powder with the pretreated leather protein powder fermentation of the hydrochloric acid of PH2 back unit weight cell count ratio.
Reference
1, Wang Houde: 40 kinds of feedstuff raw material crude protein digestibility experiments experiment reports (restricted data), Tianjin Medical College, 1989,6.
2, Wang Houde: the solid fermentation of fodder yeast and submerged fermentation, " fodder industry ", 1993, NO3
3, flood is being put down: " feedstuff raw material guide " (containing additive), Maritime Press, nineteen ninety, chapter 4 " animal raw materials "
4, " feed " Hubei science and technology press, 1992.
Claims (6)
1, biological active animal albumen powder, it is characterized in that: with the EM-1 fungus strain of commercially available import and the elite domestic probiotic bacteria of three strains, two saccharomycetes and a strain mould is fermented bacterium, connect the special zymotechnique of quadrature ratio combination back utilization to take off chromium Lan Pipi powder, rawhide skin powder (all through the persalt pre-treatment) and animal blood be base-material and be aided with raw material that cottonseed cake, rapeseed cake, Zein powder, feather meal, wheat bran, Semen Maydis powder and ammonium sulfate (inorganic salt) formed and carry out blended solid according to a certain ratio and ferment, back drying, pulverizing and the product that forms.
2, according to its special production technique feature of the described biological active animal albumen powder of claim 1 be: with the EM-1 fungus strain of commercially available import and the elite domestic probiotic bacteria of three strains, two saccharomycetes and a strain mould are fermented bacterium, connect the special zymotechnique of quadrature ratio combination back utilization to taking off chromium Lan Pipi powder, rawhide skin powder (all through the persalt pre-treatment) and animal blood are base-material and are aided with cottonseed cake, rapeseed cake, Zein powder, feather meal, wheat bran, the raw material that Semen Maydis powder and ammonium sulfate (inorganic salt) are formed mixes consubstantiality fermentation, back drying according to a certain ratio, pulverizing forms; Wherein said fermented bacterium and Orthogonal Composite ratio thereof are meant respectively:
(1) EM-1 fungus strain: draw from Japan, constitute, in Orthogonal Composite, account for 50% by 86 probiotics;
The domestic probiotic bacteria of (2) three strains:
Bifidus bacillus (Bifidobacterium sp.) accounts for 10%,
Bacillus cereus (Bacillus cereus) accounts for 10%,
Bacillus licheniformis (Bacillus lichenis) accounts for 10%,
(3) two saccharomycetes:
Endomycopsis Fibnligera (Endomycopsis fibuligera) accounts for 10%,
Candida tropicalis (Candida tropicalis) accounts for 5%;
(4) one strain moulds:
Neuraspora crassa (Neurospora crassa) accounts for 5%;
3, according to its special production technique feature of the described biological active animal albumen powder of claim 2 be: said the pre-treatment of taking off chromium Lan Pipi powder and rawhide skin powder is meant in 1: 1 ratio the technical hydrochloric acid of thousandth concentration to be admixed before the fermentation take off in chromium (Cr) Lan Pipi powder and the rawhide skin powder, at room temperature stacked two hours.
4, according to its special production technique feature of the described biological active animal albumen powder of claim 2 be: certain proportioning of said fermentation raw material is meant through pretreated leather protein powder 50%, fresh animal blood 50% (be converted into skeuomorph and account for 10% in fact) cottonseed cake 20%, rapeseed cake 5%, Zein powder 5%, feather meal 5%, Semen Maydis powder 3%, wheat bran 1%, ammonium sulfate 1%.
5, according to its special production technique feature of the described bioactive animal albumen powder of claim 2 be: said special zymotechnique is meant in material-water ratio to be 1: 0.5 ratio and material, stir after sterilize, cool off the back inoculation, adopt thick-layer ventilation technology, but strict control ventilation, to satisfy the growth conditions of anerobe and aerobic bacteria, fermented 24-36 hour.
6, according to its special production technique feature of the described biological active animal albumen powder of claim 2 be: said oven dry means adopts the oven dry of drum-type technology, and its gas flow temperature is no more than 90 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95106362A CN1144614A (en) | 1995-06-13 | 1995-06-13 | Biological active animal albumen powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95106362A CN1144614A (en) | 1995-06-13 | 1995-06-13 | Biological active animal albumen powder |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1144614A true CN1144614A (en) | 1997-03-12 |
Family
ID=5075895
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95106362A Pending CN1144614A (en) | 1995-06-13 | 1995-06-13 | Biological active animal albumen powder |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1114352C (en) * | 2000-09-13 | 2003-07-16 | 山东大学 | Process for preparing nutritive activated peptide as additive |
| CN1313568C (en) * | 2004-06-01 | 2007-05-02 | 曹建国 | Process for preparing biological diesel by repeseed fermenting method |
| CN104273404A (en) * | 2014-10-10 | 2015-01-14 | 安徽省农业科学院畜牧兽医研究所 | Method for detoxification of rapeseed cake meal by microbial solid-state fermentation |
-
1995
- 1995-06-13 CN CN95106362A patent/CN1144614A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1114352C (en) * | 2000-09-13 | 2003-07-16 | 山东大学 | Process for preparing nutritive activated peptide as additive |
| CN1313568C (en) * | 2004-06-01 | 2007-05-02 | 曹建国 | Process for preparing biological diesel by repeseed fermenting method |
| CN104273404A (en) * | 2014-10-10 | 2015-01-14 | 安徽省农业科学院畜牧兽医研究所 | Method for detoxification of rapeseed cake meal by microbial solid-state fermentation |
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