CN114460323B - Full-automatic blood sample detection equipment and detection method thereof - Google Patents
Full-automatic blood sample detection equipment and detection method thereof Download PDFInfo
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- CN114460323B CN114460323B CN202111332757.7A CN202111332757A CN114460323B CN 114460323 B CN114460323 B CN 114460323B CN 202111332757 A CN202111332757 A CN 202111332757A CN 114460323 B CN114460323 B CN 114460323B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1004—Cleaning sample transfer devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
- G01N35/1016—Control of the volume dispensed or introduced
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00306—Housings, cabinets, control panels (details)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
- G01N2035/00495—Centrifuges
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
- G01N2035/00534—Mixing by a special element, e.g. stirrer
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1034—Transferring microquantities of liquid
- G01N2035/1039—Micropipettes, e.g. microcapillary tubes
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The invention discloses a full-automatic blood sample detection device, which comprises: an outer housing; a reaction mixing assembly rotatably disposed within one side of the interior of the outer housing, the reaction mixing assembly having a plurality of sample tubes disposed in a circumferential array therein; reagent adding means vertically installed in the outer housing above the reaction mixing assembly; the quantitative liquid taking component is arranged above the inner part of the outer shell in a relatively transverse sliding manner, and performs precision liquid pumping after preliminary reaction and mixing of a blood sample in a sample test tube; the inner detection analyzer is arranged at one side, far away from the reaction mixing assembly, of the outer shell and can analyze and detect the reacted blood sample; and the waste liquid collecting cylinder is vertically arranged on one side, close to the inner detection analyzer, of the outer shell, and the upper end of the waste liquid collecting cylinder is connected with a collecting cover in a sealing manner.
Description
Technical Field
The invention belongs to the technical field of medical detection equipment, and particularly relates to full-automatic blood sample detection equipment and a detection method thereof.
Background
In the process of medical operation, doctors often need to collect blood of patients and separate serum from the collected blood, the special operation is that a professional sucks out reagents by using a special pipette and other devices, then the blood sample is separated into serum and hemoglobin by manual operation, then the separated serum sample is injected into a diluent to be detected for uniform mixing, the change condition of the serum sample is observed and the content or the component of the serum sample is measured by a detection system, and the existing serum separation operation flow is completely performed manually. The manual serum separation action is time-consuming and labor-consuming, and more importantly, is prone to sample contamination. In the multi-sample detection, the detection result is affected if the phenomenon of cross contamination is easily caused by improper cleaning; accordingly, a fully automatic blood sample testing device and a testing method thereof are provided by those skilled in the art to solve the above-mentioned problems.
Disclosure of Invention
In order to achieve the above purpose, the present invention provides the following technical solutions: a fully automatic blood sample testing apparatus, comprising:
an outer housing;
a reaction mixing assembly rotatably disposed within one side of the interior of the outer housing, the reaction mixing assembly having a plurality of sample tubes disposed in a circumferential array therein;
reagent adding means vertically installed in the outer housing above the reaction mixing assembly;
the quantitative liquid taking component is arranged above the inner part of the outer shell in a relatively transverse sliding manner, and performs precision liquid pumping after preliminary reaction and mixing of a blood sample in a sample test tube;
the inner detection analyzer is arranged at one side, far away from the reaction mixing assembly, of the outer shell and can analyze and detect the reacted blood sample; and
the waste liquid collecting cylinder is vertically arranged on one side, close to the inner detection analyzer, of the outer shell, and the upper end of the waste liquid collecting cylinder is connected with a collecting cover in a sealing mode.
Further, preferably, the reaction mixing component includes:
the positioning seat is embedded and fixed in the outer shell;
the rotating shaft is vertically arranged in the positioning seat in a relatively rotatable manner through a bearing;
the inner driving motor is arranged in the outer shell, and the output end of the inner driving motor is connected with the rotating shaft through a transmission belt for transmission and drives the rotating shaft to rotate in a directional circumference manner;
the diameter-adjusting disc piece is coaxially arranged above the rotating shaft; and
the mixing shaking-up assemblies are arranged in a plurality of circumferential arrays, and each mixing shaking-up assembly clamps and positions the sample test tubes respectively.
Further, preferably, the diameter-adjusting disc member includes:
a carrying tray;
the outer shaft couplings are circumferentially arranged, the limiting parts of the outer shaft couplings which can slide relatively are arranged in the bearing plate, and pin keys are vertically fixed on the outer shaft couplings; and
the outer rotating disc is coaxially arranged on the bearing disc in a relatively rotatable manner, a plurality of sliding holes corresponding to the pin keys one by one are formed in the outer rotating disc, and the pin keys are arranged in the sliding holes in a sliding limiting manner.
Further, preferably, the mixing and shaking-up assembly includes:
a fixing seat;
the side link frame is vertically fixed on one side of the upper end face of the fixed seat, the cross section of the side link frame is of an inverted L-shaped structure, and a mounting concave position is arranged in the side link frame;
the inner jacket is coaxially arranged in the mounting concave position, and a plurality of rotors are arranged on the outer circumference of the inner jacket in a row and are limited by the rotors to rotate on the side link frame;
the friction ring piece is coaxially arranged in the inner jacket;
the central shaft is arranged on the fixed seat in a relatively rotatable manner, and a supporting rod is obliquely fixed at the upper end of the central shaft; and
and the telescopic connecting rod is hinged to one end of the supporting rod, and one end of the telescopic connecting rod is connected with the inner jacket.
Further, preferably, the telescopic connecting rod is configured into a two-section telescopic supporting rod structure, and the inner wall of the cross section of the installation concave position is in an arc-shaped structure.
Further, preferably, the quantitative liquid taking assembly includes:
the transmission bar is transversely fixed in the outer shell;
the fixed shaft bracket is vertically and relatively slidably arranged on the transmission connecting rod, a linkage belt is rotatably sleeved on the transmission connecting rod, and the linkage belt drives the fixed shaft bracket to transversely slide;
the inner mounting seat is arranged on the fixed shaft bracket in a relatively sliding manner;
the main rotating wheel is rotatably arranged on the fixed shaft bracket, a connecting rope is wound on the main rotating wheel, and one end of the connecting rope is connected with the inner mounting seat; and
the liquid taking device is vertically fixed on the inner mounting seat.
Further, preferably, the liquid taking device includes:
a main storage pipe is fixed;
the liquid taking needle piece is coaxially arranged at one end of the storage main pipe;
the diverter is connected to the upper end of the storage main pipe;
the rough liquid extracting pump and the fine liquid extracting pump are respectively and obliquely communicated with the two side ends of the flow divider; and
the cleaning component is sleeved outside the storage main pipe and is used for cleaning the inside after the storage main pipe finishes the blood sample extraction and discharge.
Further, preferably, the cleaning assembly includes:
the outer sealing sleeve is coaxially and hermetically sleeved outside the storage main pipe, a plurality of drainage holes are circumferentially formed in the storage main pipe and positioned at the outer sealing sleeve, and a water supply pipe is communicated with the outer sealing sleeve;
the inner guide pipe is vertically and relatively slidably arranged in the storage main pipe, a plurality of inner springs are symmetrically arranged outside the inner guide pipe, and one end of each inner spring is connected with the storage main pipe;
the sealing sleeve is arranged in the storage main pipe and positioned outside the inner guide pipe, and a plurality of inner row holes are formed in the circumference of one end, close to the sealing sleeve, of the inner guide pipe; and
the plugging disc is vertically fixed in the inner guide pipe, and a telescopic pipe is further connected between the inner guide pipe and the storage main pipe.
A method of testing a fully automated blood sample testing device, comprising the steps of:
s1, clamping and placing a sample; the sample test tubes are preliminarily clamped through the inner jacket, so that a plurality of sample test tubes can be circumferentially distributed on the diameter-adjusting disc, pre-stored abandoned sample liquid can be reserved between two sections of sample branch pipes, and cross contamination in sample extraction is avoided;
s2, adding and mixing; the diameter-adjusting disc piece controls the sample test tubes to move to the lower part of the reagent adding device, the reagent adding device adds hemolytic agents to the sample test tubes one by one at the moment, and the central shaft can drive the sample test tubes to rotate and shake evenly under the rotating action through the telescopic connecting rod; the inclination angle of the sample test tube main body can be adjusted by the telescopic action of the telescopic connecting rod, and the diameter-adjusting disc piece can correspondingly adjust the size of the centrifugal rotation outer diameter;
s3, accurately pumping liquid; the liquid taking device drives the fixed shaft bracket to transversely slide to the upper part of the sample test tube through the linkage belt, and slides to extend into the sample test tube, the rough liquid drawing pump preferentially performs primary liquid drawing, and the fine liquid drawing pump performs micro-extraction, so that the extraction precision is ensured, and the blood sample is stored in the storage main tube;
s4, sample analysis and detection; the liquid taking device discharges the blood sample to the internal detection analyzer for analysis and detection;
s5, cleaning the pipe wall; the liquid taking device is moved to the position of the waste liquid collecting cylinder, at the moment, the water supply pipe is used for conveying clean water to the outer sealing sleeve at constant pressure, the inner guide pipe is vertically and relatively moved under the discharge pressure, so that the discharge holes are communicated with the inner discharge holes, high-pressure clean water is sent to the storage main pipe, at the moment, the sealing disc can be used for sealing and sealing the lower end of the storage main pipe, intermittent water conveying cleaning is formed, and when the water supply pipe water conveying pressure reaches a certain value, the sealing disc can be completely sealed at the port of the storage main pipe, and clean water is completely filled in the storage main pipe; thereby improving the cleaning effect of the pipe wall;
s6, repeating the steps S3-S5, and performing full-automatic blood sample detection.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, aiming at serum separation operation, a plurality of mixing shaking components in a diameter-adjusting disc piece clamp a sample test tube, hemolytic agents are injected into the sample test tube one by one through a reagent adding device, and centrifugal separation is carried out after injection is completed, so that automatic blood sample detection is realized; in particular, in blood sample drawing, to prevent cross-contamination of multiple blood samples, the cleaning assembly is capable of performing a fill-flow cleaning operation on the interior of the storage main tube; meanwhile, a pre-stored abandoned sample liquid can be reserved between the two sections of sample branch pipes, so that the accuracy of the detection result is improved.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic structural view of a reaction mixing element according to the present invention;
FIG. 3 is a schematic diagram of a mixing and shaking assembly according to the present invention;
FIG. 4 is a schematic diagram of a quantitative liquid-taking component according to the present invention;
FIG. 5 is a schematic view of a liquid-extracting apparatus according to the present invention;
FIG. 6 is a schematic view of a cleaning assembly according to the present invention;
in the figure: the device comprises a 1 outer casing, a 2 reaction mixing component, a 21 positioning seat, a 22 rotating shaft, a 23 conveying belt, a 3 reagent adding device, a 4 and 41 conveying bar, a 42 fixing shaft bracket, a 43 inner installation seat, a 44 main rotating wheel, a 5 inner detection analyzer, a 51 waste liquid collecting cylinder, a 52 collecting cover, a 6 diameter-adjusting disc piece, a 61 bearing disc, a 62 outer coupling, a 63 outer rotating disc, a 7 mixing shaking component, a 71 fixing seat, a 72 side coupling frame, a 73 inner jacket, a 74 rotor, a 75 friction ring piece, a 76 central shaft, a 77 telescopic connecting rod, an 8 liquid taking device, a 81 positioning main pipe, a 82 liquid taking needle piece, a 83 diverter, a 84 rough liquid pumping pump, a 85 fine liquid pumping pump, a 9 cleaning component, a 91 outer sealing sleeve, a 92 inner guide pipe, a 93 inner spring, a 94 sealing sleeve piece, a 95 telescopic pipe and a 96 sealing disc.
Detailed Description
Referring to fig. 1, in an embodiment of the present invention, a fully automatic blood sample testing apparatus includes:
an outer casing 1;
a reaction mixing component 2 rotatably arranged at one side of the inner part of the outer casing 1, wherein a plurality of sample test tubes are arranged in a circumferential array in the reaction mixing component 2;
reagent adding means 3 vertically installed in the outer casing 1 above the reaction mixing assembly 2;
the quantitative liquid taking component 4 is arranged above the inner part of the outer shell 1 in a relatively transverse sliding manner, and the quantitative liquid taking component 4 performs accurate liquid pumping after preliminary reaction and mixing of a blood sample in a sample tube;
an internal detection analyzer 5 installed at a side of the external housing 1 far from the reaction mixing assembly 2, the internal detection analyzer 5 being capable of analyzing and detecting the reacted blood sample; and
a waste liquid collecting cylinder 51 vertically installed at one side of the outer housing 1 near the inner detection analyzer 5, and a collecting cover 52 hermetically connected to the upper end of the waste liquid collecting cylinder 51.
In this embodiment, the reaction mixing element 2 comprises:
a positioning seat 21 embedded and fixed in the outer casing 1;
a rotation shaft 22 vertically arranged in the positioning seat 21 by a bearing capable of relatively rotating;
the inner driving motor is arranged in the outer shell 1, and the output end of the inner driving motor is connected and transmitted with the rotating shaft 22 through a transmission belt 23 and drives the rotating shaft 22 to rotate in a directional circumference manner;
a diameter-adjusting disc 6 coaxially disposed above the rotation shaft 22; and
the mixing shaking-up assembly 7 is a plurality of circumferential arrays, each mixing shaking-up assembly 7 clamps and positions sample test tubes respectively, wherein the diameter-adjusting disc piece can horizontally control each sample test tube to carry out transverse displacement, and particularly, the centrifugal radius is changed in preliminary reaction mixing of blood samples, and the centrifugal separation strength is adjusted.
As a preferred embodiment, the dial 6 comprises:
a carrier tray 61;
the outer shaft couplings 62 are circumferentially arranged, the limiting positions of the outer shaft couplings 62 which can slide relatively are arranged in the bearing disc 61, and pin keys are vertically fixed on the outer shaft couplings 62; and
the outer rotating disc 63 is coaxially arranged on the bearing disc 61 in a relatively rotatable manner, a plurality of sliding holes corresponding to the pin keys one by one are formed in the outer rotating disc 63, and the pin keys are arranged in the sliding holes in a sliding limiting manner.
In this embodiment, the mixing and shaking-up assembly 7 includes:
a fixing base 71;
the side link frame 72 is vertically fixed on one side of the upper end face of the fixed seat 71, the cross section of the side link frame 72 is in an inverted L-shaped structure, and an installation concave position is arranged in the side link frame 72;
an inner jacket 73 coaxially arranged in the mounting recess, wherein a plurality of rotors 74 are arranged on the outer circumference of the inner jacket 73, and the rotors 74 are limited to rotate on the side link frame 72;
a friction ring 75 coaxially disposed within said inner jacket 73;
the central shaft 76 is rotatably arranged on the fixed seat 71, and a supporting rod is obliquely fixed at the upper end of the central shaft 76; and
and a telescopic connecting rod 77 hinged to one end of the supporting rod, wherein one end of the telescopic connecting rod 77 is connected with the inner jacket 73.
In this embodiment, the telescopic link 77 is configured as a two-section telescopic strut structure, and the inner wall of the cross section of the installation recess is in an arc structure, so that the sample tube can be in a local inclined state in the side-link frame by preferentially performing axial telescopic action of the telescopic link, and at this time, the central shaft drives the sample tube to rotate circumferentially under the rotation action, so as to improve the mixing separation effect.
In this embodiment, the quantitative liquid taking assembly 4 includes:
a transmission bar 41 transversely fixed in the outer casing 1;
the fixed shaft frame 42 is vertically and relatively slidably arranged on the transmission connecting rod 41, a linkage belt 45 is rotatably sleeved on the transmission connecting rod 41, and the linkage belt 45 drives the fixed shaft frame 42 to transversely slide;
an inner mounting seat 43 slidably disposed on the fixed shaft frame 42;
the main rotating wheel 44 is rotatably arranged on the fixed shaft bracket 42, a connecting rope is wound on the main rotating wheel 44, and one end of the connecting rope is connected with the inner mounting seat 43; and
the liquid taking device 8 is vertically fixed on the inner mounting seat 43.
As a preferred embodiment, the liquid-taking device 8 includes:
a storage main pipe 81;
the liquid taking needle 82 is coaxially arranged at one end of the storage main pipe 81;
a diverter 83 connected to the upper end of the storage main pipe 81;
a rough liquid pump 84 and a fine liquid pump 85 which are respectively and obliquely communicated with the two side ends of the flow divider 83; and
the cleaning component 9 is sleeved outside the storage main pipe 81, and internal cleaning is performed after the storage main pipe 81 finishes blood sample extraction and discharge.
In this embodiment, the cleaning assembly 9 includes:
the outer sealing sleeve 91 is coaxially and hermetically sleeved outside the storage main pipe 81, a plurality of drainage holes are circumferentially formed in the storage main pipe 81 at the outer sealing sleeve 91, and a water supply pipe is communicated with the outer sealing sleeve 91;
the inner guide pipe 92 is vertically and relatively slidably arranged in the storage main pipe 81, a plurality of inner springs 93 are symmetrically arranged outside the inner guide pipe 92, and one end of each inner spring 93 is connected with the storage main pipe 81;
the sealing sleeve 94 is arranged in the storage main pipe 81 and positioned outside the inner conduit 92, and a plurality of inner row holes are formed in the circumference of one end, close to the sealing sleeve 94, of the inner conduit 92; and
the sealing disc 96 is vertically fixed in the inner conduit 92, the telescopic tube 95 is further connected between the inner conduit 92 and the fixed storage main tube 81, when the end part of the inner conduit and the liquid taking needle are cleaned, cleaning water is conveyed to the outer sealing sleeve at constant pressure through the water supply pipe, at the moment, the inner conduit is vertically and relatively displaced under the discharge pressure, the discharge holes are communicated with the inner discharge holes, the cleaning water locally flows into the inner conduit, and after the pressure is reduced to a certain value, the inner conduit vertically slides and resets, intermittent water conveying cleaning is formed, repeated flushing operation is conveniently carried out on the end part of the inner conduit and the liquid taking needle, and when the water conveying pressure of the water supply pipe reaches a certain value, the sealing disc can be completely sealed at the end part of the fixed storage main tube, and the cleaning water is completely filled in the fixed storage main tube, so that the cleaning of the walls of the fixed storage main tube and the inner conduit is realized.
In this embodiment, a detection method of a fully automatic blood sample detection device includes the following steps:
s1, clamping and placing a sample; the sample test tubes are preliminarily clamped through the inner jacket 73, so that a plurality of sample test tubes can be circumferentially distributed on the diameter-adjusting disc member 6, pre-stored abandoned sample liquid can be reserved between two sections of sample branch pipes, and cross contamination in sample extraction is avoided;
s2, adding and mixing; the diameter-adjusting disc piece 6 controls the sample test tubes to move to the lower part of the reagent adding device 3, the reagent adding device 3 adds hemolytic agents into the sample test tubes one by one at the moment, and the central shaft 76 can drive the sample test tubes to rotate and shake evenly through the telescopic connecting rod 77 under the rotating action; the inclination angle of the sample test tube main body can be adjusted by the telescopic action of the telescopic connecting rod 77 before, and the diameter-adjusting disc 6 can correspondingly adjust the size of the centrifugal rotation outer diameter;
s3, accurately pumping liquid; the liquid taking device 8 drives the fixed shaft bracket to transversely slide to the upper part of the sample test tube through the linkage belt 45, and slides to extend into the sample test tube, the rough liquid extracting pump 84 preferentially performs primary liquid extraction, and the fine liquid extracting pump 85 performs micro liquid extraction, so that the extraction precision is ensured, and the blood sample is stored in the storage main tube 81;
s4, sample analysis and detection; the liquid taking device 8 discharges the blood sample to an internal detection analyzer for analysis and detection;
s5, cleaning the pipe wall; the liquid taking device 8 is moved to the waste liquid collecting cylinder, at the moment, the water supply pipe is used for conveying clean water to the outer sealing sleeve 91 at constant pressure, the inner guide pipe 92 is vertically and relatively moved under the discharge pressure, so that the discharge holes are communicated with the inner discharge holes, high-pressure clean water is sent to the storage main pipe 81, at the moment, the lower end of the storage main pipe 81 can be sealed and blocked by the blocking disc 96, intermittent water conveying cleaning is formed, and when the water supply pipe water conveying pressure reaches a certain value, the port of the storage main pipe 81 can be completely blocked by the blocking disc 96, and clean water is completely filled in the storage main pipe 81; thereby improving the cleaning effect of the pipe wall;
s6, repeating the steps S3-S5, and performing full-automatic blood sample detection.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (6)
1. A full-automatic blood sample testing device, characterized in that: it comprises the following steps:
an outer casing (1);
a reaction mixing component (2) which is arranged on one side of the inner part of the outer shell (1) in a relatively rotatable manner, wherein a plurality of sample test tubes are arranged in a circumferential array in the reaction mixing component (2);
a reagent adding device (3) vertically installed in the outer casing (1) above the reaction mixing component (2);
the quantitative liquid taking component (4) can be arranged above the inner part of the outer shell (1) in a relatively transverse sliding manner, and the quantitative liquid taking component (4) performs accurate liquid pumping after preliminary reaction and mixing of a blood sample in a sample test tube;
an inner detection analyzer (5) mounted on a side of the outer housing (1) away from the reaction mixing assembly (2), the inner detection analyzer (5) being capable of analyzing and detecting the reacted blood sample; and
the waste liquid collecting cylinder (51) is vertically arranged at one side, close to the inner detection analyzer (5), of the outer shell (1), and the upper end of the waste liquid collecting cylinder (51) is connected with a collecting cover (52) in a sealing manner;
the reaction mixing component (2) comprises:
the positioning seat (21) is embedded and fixed in the outer shell (1);
the rotating shaft (22) is vertically arranged in the positioning seat (21) in a relatively rotatable manner through a bearing;
the inner driving motor is arranged in the outer shell (1), and the output end of the inner driving motor is connected and transmitted with the rotating shaft (22) through a transmission belt (23) and drives the rotating shaft (22) to rotate in a directional circumference manner;
a diameter-adjusting disc (6) coaxially arranged above the rotating shaft (22); and
the mixing shaking-up assemblies (7) are arranged in a plurality of circumferential arrays, and each mixing shaking-up assembly (7) respectively clamps and positions the sample test tubes;
the mixing shaking-up assembly (7) comprises:
a fixed seat (71);
the side link frame (72) is vertically fixed on one side of the upper end face of the fixed seat (71), the cross section of the side link frame (72) is of an inverted L-shaped structure, and a mounting concave position is arranged in the side link frame (72);
the inner jacket (73) is coaxially arranged in the mounting concave position, a plurality of rotors (74) are arranged on the outer circumference of the inner jacket (73), and the rotors (74) are limited to rotate on the side link frame (72);
a friction ring member (75) coaxially disposed in the inner jacket (73);
the central shaft (76) is arranged on the fixed seat (71) in a relatively rotatable manner, and a stay bar is obliquely fixed at the upper end of the central shaft (76); and
the telescopic connecting rod (77) is hinged to one end of the supporting rod, and one end of the telescopic connecting rod (77) is connected with the inner jacket (73);
the telescopic connecting rod (77) is constructed into a two-section telescopic supporting rod structure, and the inner wall of the cross section of the installation concave position is of an arc-shaped structure.
2. A fully automated blood sample testing device according to claim 1, wherein: the diameter-adjusting disc member (6) comprises:
a carrier tray (61);
the outer shaft couplings (62) are circumferentially arranged, limiting positions of the outer shaft couplings (62) which can slide relatively are arranged in the bearing plate (61), and pin keys are vertically fixed on the outer shaft couplings (62); and
the outer rotating disc (63) can rotate relatively and is coaxially arranged on the bearing disc (61), a plurality of sliding holes corresponding to the pin keys one by one are formed in the outer rotating disc (63), and the pin keys are arranged in the sliding holes in a sliding limiting mode.
3. A fully automated blood sample testing device according to claim 1, wherein: the quantitative liquid taking component (4) comprises:
a transmission bar (41) transversely fixed in the outer casing (1);
the fixed shaft bracket (42) is vertically and relatively slidably arranged on the transmission connecting rod (41), a linkage belt (45) is rotatably sleeved on the transmission connecting rod (41), and the linkage belt (45) drives the fixed shaft bracket (42) to transversely slide;
the inner mounting seat (43) is arranged on the fixed shaft bracket (42) in a relatively sliding manner;
the main rotating wheel (44) is rotatably arranged on the fixed shaft bracket (42), a connecting rope is wound on the main rotating wheel (44), and one end of the connecting rope is connected with the inner mounting seat (43); and
the liquid taking device (8) is vertically fixed on the inner mounting seat (43).
4. A fully automated blood sample testing device according to claim 3, wherein: the liquid taking device (8) comprises:
a storage main pipe (81);
the liquid taking needle (82) is coaxially arranged at one end of the storage main pipe (81);
a diverter (83) connected to the upper end of the storage main pipe (81);
a rough liquid extracting pump (84) and a refined liquid extracting pump (85) are respectively and obliquely communicated with two side ends of the flow divider (83); and
the cleaning component (9) is sleeved outside the storage main pipe (81), and internal cleaning is performed after the storage main pipe (81) finishes blood sample extraction and discharge.
5. A fully automated blood sample testing device according to claim 4, wherein: the cleaning assembly (9) comprises:
the outer sealing sleeve (91) is coaxially and hermetically sleeved outside the storage main pipe (81), a plurality of drainage holes are circumferentially formed in the storage main pipe (81) and located at the outer sealing sleeve (91), and the outer sealing sleeve (91) is communicated with a water supply pipe;
the inner guide pipe (92) is vertically and relatively slidably arranged in the storage main pipe (81), a plurality of inner springs (93) are symmetrically arranged outside the inner guide pipe (92), and one end of each inner spring (93) is connected with the storage main pipe (81);
the sealing sleeve (94) is arranged in the storage main pipe (81) and positioned outside the inner guide pipe (92), and a plurality of inner row holes are formed in the circumference of one end, close to the sealing sleeve (94), of the inner guide pipe (92); and
the plugging disc (96) is vertically fixed in the inner guide pipe (92), and a telescopic pipe (95) is further connected between the inner guide pipe (92) and the storage main pipe (81).
6. The method for detecting a fully automatic blood sample according to claim 5, wherein: which comprises the following steps:
s1, clamping and placing a sample; the sample test tubes are preliminarily clamped through the inner jacket (73), so that a plurality of sample test tubes can be circumferentially distributed on the diameter-adjusting disc (6);
s2, adding and mixing; the diameter-adjusting disc (6) controls each sample test tube to move below the reagent adding device (3), the reagent adding device (3) adds hemolytic agent into the sample test tubes one by one at the moment, and the central shaft (76) can drive the sample test tubes to rotate and shake evenly under the rotating action through the telescopic connecting rod (77); the inclination angle of the sample test tube main body is adjusted by the telescopic action of the telescopic connecting rod (77), and the diameter-adjusting disc (6) correspondingly carries out centrifugal rotation outer diameter adjustment;
s3, accurately pumping liquid; the liquid taking device (8) drives the fixed shaft bracket to transversely slide to the upper part of the sample test tube through the linkage belt (45) and slide to extend into the sample test tube, the rough liquid extracting pump (84) preferentially performs primary liquid extraction, and the fine liquid extracting pump (85) performs micro extraction to ensure the extraction precision, so that a blood sample is stored in the storage main pipe (81);
s4, sample analysis and detection; the liquid taking device (8) discharges the blood sample to an internal detection analyzer for analysis and detection;
s5, cleaning the pipe wall; the liquid taking device (8) is displaced to the waste liquid collecting cylinder, at the moment, the water supply pipe is used for conveying clean water to the outer sealing sleeve (91) at constant pressure, the inner guide pipe (92) is vertically and relatively displaced under the discharge pressure, so that the discharge holes are communicated with the inner discharge holes, high-pressure clean water is sent to the storage main pipe (81), at the moment, the lower end of the storage main pipe (81) can be sealed and blocked by the blocking disc (96), intermittent water conveying cleaning is formed, and when the water supply pipe water conveying pressure reaches a certain value, the port of the storage main pipe (81) can be completely blocked by the blocking disc (96), and clean water is completely filled in the storage main pipe (81); thereby improving the cleaning effect of the pipe wall;
s6, repeating the steps S3-S5, and performing full-automatic blood sample detection.
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| CN109540643A (en) * | 2018-12-13 | 2019-03-29 | 深圳市海拓华擎生物科技有限公司 | Whole blood evenly mixing device |
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| CN113447668B (en) * | 2021-08-09 | 2023-04-11 | 江苏国际旅行卫生保健中心(南京海关口岸门诊部) | Liquid transfer device for medical clinical laboratory |
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