CN114456012A - Low-yield composite biological organic bacterial fertilizer for cotton fields and preparation method thereof - Google Patents
Low-yield composite biological organic bacterial fertilizer for cotton fields and preparation method thereof Download PDFInfo
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- CN114456012A CN114456012A CN202210041993.1A CN202210041993A CN114456012A CN 114456012 A CN114456012 A CN 114456012A CN 202210041993 A CN202210041993 A CN 202210041993A CN 114456012 A CN114456012 A CN 114456012A
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- 229920000742 Cotton Polymers 0.000 title claims abstract description 65
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 41
- 239000003337 fertilizer Substances 0.000 title claims abstract description 41
- 239000002131 composite material Substances 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 32
- 210000003608 fece Anatomy 0.000 claims abstract description 32
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 claims abstract description 28
- YIXJRHPUWRPCBB-UHFFFAOYSA-N magnesium nitrate Chemical compound [Mg+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O YIXJRHPUWRPCBB-UHFFFAOYSA-N 0.000 claims abstract description 26
- 241000223260 Trichoderma harzianum Species 0.000 claims abstract description 20
- 229910052742 iron Inorganic materials 0.000 claims abstract description 16
- 239000010871 livestock manure Substances 0.000 claims abstract description 16
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 claims abstract description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 14
- 229960003692 gamma aminobutyric acid Drugs 0.000 claims abstract description 14
- 239000008103 glucose Substances 0.000 claims abstract description 14
- 229920002643 polyglutamic acid Polymers 0.000 claims abstract description 14
- 229920001732 Lignosulfonate Polymers 0.000 claims abstract description 13
- 241001494479 Pecora Species 0.000 claims abstract description 13
- 239000004111 Potassium silicate Substances 0.000 claims abstract description 13
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 13
- NGLMYMJASOJOJY-UHFFFAOYSA-O azanium;calcium;nitrate Chemical compound [NH4+].[Ca].[O-][N+]([O-])=O NGLMYMJASOJOJY-UHFFFAOYSA-O 0.000 claims abstract description 13
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims abstract description 13
- 239000001506 calcium phosphate Substances 0.000 claims abstract description 13
- 229910000150 monocalcium phosphate Inorganic materials 0.000 claims abstract description 13
- 235000019691 monocalcium phosphate Nutrition 0.000 claims abstract description 13
- NNHHDJVEYQHLHG-UHFFFAOYSA-N potassium silicate Chemical compound [K+].[K+].[O-][Si]([O-])=O NNHHDJVEYQHLHG-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229910052913 potassium silicate Inorganic materials 0.000 claims abstract description 13
- 235000019353 potassium silicate Nutrition 0.000 claims abstract description 13
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000011701 zinc Substances 0.000 claims abstract description 13
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 13
- 229920001817 Agar Polymers 0.000 claims abstract description 12
- 239000001888 Peptone Substances 0.000 claims abstract description 12
- 108010080698 Peptones Proteins 0.000 claims abstract description 12
- 239000008272 agar Substances 0.000 claims abstract description 12
- 235000015278 beef Nutrition 0.000 claims abstract description 12
- 235000019319 peptone Nutrition 0.000 claims abstract description 12
- 239000001963 growth medium Substances 0.000 claims abstract description 11
- 239000011684 sodium molybdate Substances 0.000 claims abstract description 11
- 235000015393 sodium molybdate Nutrition 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims abstract description 10
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 claims abstract description 9
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000002509 fulvic acid Substances 0.000 claims abstract description 9
- 229940095100 fulvic acid Drugs 0.000 claims abstract description 9
- 239000002689 soil Substances 0.000 claims description 26
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 24
- 239000011259 mixed solution Substances 0.000 claims description 23
- 239000011265 semifinished product Substances 0.000 claims description 22
- 150000001875 compounds Chemical class 0.000 claims description 21
- 238000002156 mixing Methods 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 230000001954 sterilising effect Effects 0.000 claims description 13
- 239000000292 calcium oxide Substances 0.000 claims description 12
- 235000012255 calcium oxide Nutrition 0.000 claims description 12
- 238000005303 weighing Methods 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 10
- 238000009264 composting Methods 0.000 claims description 9
- 239000000047 product Substances 0.000 claims description 9
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 7
- 238000007873 sieving Methods 0.000 claims description 7
- 238000009331 sowing Methods 0.000 claims description 7
- 238000005507 spraying Methods 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 239000002361 compost Substances 0.000 claims description 5
- 230000007480 spreading Effects 0.000 claims description 5
- 238000003892 spreading Methods 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims 1
- 241000082085 Verticillium <Phyllachorales> Species 0.000 abstract description 16
- 238000011282 treatment Methods 0.000 abstract description 10
- 239000013043 chemical agent Substances 0.000 abstract description 6
- 241000589180 Rhizobium Species 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 239000000575 pesticide Substances 0.000 abstract description 3
- 241000894006 Bacteria Species 0.000 abstract description 2
- 238000010668 complexation reaction Methods 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 abstract description 2
- 150000007524 organic acids Chemical class 0.000 abstract description 2
- 235000005985 organic acids Nutrition 0.000 abstract description 2
- 230000007928 solubilization Effects 0.000 abstract description 2
- 238000005063 solubilization Methods 0.000 abstract description 2
- 230000002265 prevention Effects 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 6
- 241000223218 Fusarium Species 0.000 description 6
- 239000003513 alkali Substances 0.000 description 5
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 4
- 238000003973 irrigation Methods 0.000 description 4
- 230000002262 irrigation Effects 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 235000021049 nutrient content Nutrition 0.000 description 4
- 239000011591 potassium Substances 0.000 description 4
- 229910052700 potassium Inorganic materials 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000035558 fertility Effects 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 208000035240 Disease Resistance Diseases 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 239000003124 biologic agent Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000003050 macronutrient Effects 0.000 description 1
- 235000021073 macronutrients Nutrition 0.000 description 1
- 229910001425 magnesium ion Inorganic materials 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/10—Addition or removal of substances other than water or air to or from the material during the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Soil Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a low-yield composite biological organic bacterial fertilizer for cotton fields, which comprises the following raw materials in parts by weight: 10-20 parts of fulvic acid diamineiron, 5-10 parts of chelated iron, 6-15 parts of calcium ammonium nitrate, 5-10 parts of monocalcium phosphate, 2-4 parts of magnesium nitrate, 2-5 parts of sodium molybdate, 2-4 parts of anhydrous potassium silicate, 2-4 parts of zinc lignosulfonate, 25-30 parts of dried cow manure or dried sheep manure, 5-10 parts of glucose, 20-30 parts of gamma-polyglutamic acid, 15-20 parts of beef extract peptone agar culture medium, 20-25 parts of trichoderma harzianum, 10-20 parts of rhizobium and 2-4 parts of gamma-aminobutyric acid. The invention utilizes the complexation solubilization of organic matters and organic acids to combine with beneficial microorganism bacteria, and solves the problems that chemical agents in the prior art can not ensure the safety of agricultural ecological environment, the prevention and the treatment of cotton verticillium wilt and blight and the yield increase of low-yield cotton fields through the combination of pesticide and fertilizer. The invention also discloses a preparation method of the low-yield composite biological organic bacterial fertilizer for the cotton fields.
Description
Technical Field
The invention belongs to the technical field of soil utilization, and relates to a low-yield composite biological organic bacterial fertilizer for cotton fields.
Background
The cotton industry plays a significant role in Xinjiang economy, and the cotton yield value accounts for 1/2 of the total agricultural yield value of all-Xinjiang. In recent years, verticillium wilt and blight are becoming more and more serious due to unreasonable fertilizer application and high-density mulching cultivation in successive years, high saline-alkali content of soil in low-and medium-yield fields and the like. The chemical agents for preventing and treating cotton verticillium wilt and fusarium wilt are various in types, but none of the chemical agents can effectively control the expansion of verticillium wilt and fusarium wilt, and the application of the chemical agents also poisons the environment and causes environmental pollution. In recent years, a method for controlling a biological agent mainly comprising microorganisms has a good effect on controlling verticillium wilt and fusarium wilt.
The synergistic improvement of the low-yield field productivity and the plant disease resistance by utilizing the interaction mechanism of a plant-soil-microorganism system has become an international new trend. The existing chemical agents and biological agents obviously cannot meet the technical requirements of ensuring the safety of agricultural environment, preventing and treating cotton verticillium wilt and blight and improving the cotton field productivity.
Disclosure of Invention
The invention aims to provide a compound biological organic bacterial fertilizer for a low-yield cotton field, which solves the problems that the chemical agent has independent function and seriously pollutes the environment, and cannot meet the requirements of preventing and treating cotton verticillium wilt and improving the low-yield cotton field capacity at the same time by combining the complexation solubilization of organic matters and organic acids with the coupling promotion effect of beneficial microbial agents on the low-yield cotton field capacity improvement and integrating pesticide and fertilizer.
The invention also aims to provide a method for preparing the low-yield composite biological organic bacterial fertilizer for the cotton fields.
The invention adopts the technical scheme that the low-yield composite biological organic bacterial fertilizer for the cotton field is sequentially composed of the following raw materials in parts by weight: 10-20 parts of fulvic acid diamineiron, 5-10 parts of chelated iron, 6-15 parts of calcium ammonium nitrate, 5-10 parts of monocalcium phosphate, 2-4 parts of magnesium nitrate, 2-5 parts of sodium molybdate, 2-4 parts of anhydrous potassium silicate, 2-4 parts of zinc lignosulfonate, 1-3 parts of quick lime, 25-30 parts of dried cow manure or dried sheep manure, 5-10 parts of glucose, 20-30 parts of gamma-polyglutamic acid, 15-20 parts of beef extract peptone agar culture medium, 20-25 parts of trichoderma harzianum, 10-20 parts of rhizobia and 2-4 parts of gamma-aminobutyric acid.
Preferably, the number of viable bacteria of Trichoderma harzianum is greater than or equal to 200 hundred million/g.
The invention adopts another technical scheme that a method for manufacturing a low-yield composite biological organic bacterial fertilizer for cotton fields is implemented according to the following steps:
step 1, weighing the following raw materials in parts by weight: 10-20 parts of fulvic acid diamineiron, 5-10 parts of chelated iron, 6-15 parts of calcium ammonium nitrate, 5-10 parts of monocalcium phosphate, 2-4 parts of magnesium nitrate, 2-5 parts of sodium molybdate, 2-4 parts of anhydrous potassium silicate, 2-4 parts of zinc lignosulfonate, 1-3 parts of quick lime, 25-30 parts of dried cow dung or dried sheep dung, 5-10 parts of glucose, 20-30 parts of gamma-polyglutamic acid, 15-20 parts of beef extract peptone agar culture medium, 20-25 parts of trichoderma harzianum, 10-20 parts of rhizobia and 2-4 parts of gamma-aminobutyric acid;
step 2, weighing the ferrous fulvate, chelated iron, calcium ammonium nitrate, monocalcium phosphate, magnesium nitrate, sodium molybdate, anhydrous potassium silicate, zinc lignosulfonate, quick lime and dry cow manure or dry sheep manure in the step 1, crushing, stirring, uniformly mixing, standing for 4-5 hours, granulating, drying and sterilizing to form a semi-finished product;
step 3, composting the semi-finished product obtained in the step 2 outdoors;
and 4, uniformly spraying a mixed solution (the concentration is less than or equal to 8g/L) prepared by glucose, gamma-polyglutamic acid, trichoderma harzianum, rhizobia and gamma-aminobutyric acid weighed in the step 1 on the surface of the compost obtained in the step 3, uniformly mixing the mixed solution once every 2 to 3 hours for 4 to 5 times, standing the mixture for 6 to 8 hours, bagging the mixture for later use when the mass water content of a finished product is less than 5 percent, and obtaining the composite biological organic bacterial fertilizer for the low-yield cotton fields, wherein the effective viable count of the trichoderma harzianum and the rhizobia is less than or equal to 200 hundred million/g.
Preferably, the step 2 is implemented by crushing the mixture by a crusher and sieving the crushed mixture by a 100-mesh sieve.
Preferably, the sterilization in step 2 is 120-.
Preferably, the compost in step 3 is specifically: composting the semi-finished product outdoors, turning the pile for 1-2 times when the central temperature of the pile is not less than 50 ℃ and the pile is full for 7-10 days, airing for 12-48 hours, piling the pile again, and repeatedly airing for 5-7 times and transferring to the environment of 25-30 ℃.
Preferably, the prepared low-yield composite biological organic bacterial fertilizer for cotton fields is applied by a spreading mode before sowing and soil preparation.
Preferably, the application amount of the compound biological organic bacterial fertilizer for the low-yield cotton fields is 30-50 kg/mu.
The invention has the beneficial effects that:
the compound biological organic bacterial fertilizer for the low-yield cotton fields is rich in active calcium and magnesium ions and can effectively replace soil sodium ions, so that the salinization degree of soil is reduced, the microenvironment of root areas is conditioned, and the nutrient absorption of crops is facilitated; the variety of the contained macronutrient elements and medium and micronutrient elements is comprehensive and balanced, the total nitrogen, phosphorus and potassium nutrient content is more than or equal to 20 percent, the soil fertility of the saline-alkali soil can be comprehensively improved, and the growth of crops can be powerfully promoted; the contained trichoderma harzianum and rhizobia have the functions of fixing nitrogen, dissolving potassium, resisting stress and the like.
The raw materials of the invention cooperate with each other, are easy to be absorbed by plants, and can effectively improve the disease resistance (verticillium wilt and blight) of plants and improve the soil fertility. The invention has the characteristics of pesticide and fertilizer combination, has no toxic action on soil, crops and environment, is simple and convenient to operate, has high cost performance, and achieves the unification of economic, ecological and environmental benefits.
The present invention will be described in detail with reference to the following embodiments.
The invention relates to a low-yield composite biological organic bacterial fertilizer for cotton fields, which sequentially comprises the following raw materials in parts by weight: 10-20 parts of fulvic acid diamineiron, 5-10 parts of chelated iron, 6-15 parts of calcium ammonium nitrate, 5-10 parts of monocalcium phosphate, 2-4 parts of magnesium nitrate, 2-5 parts of sodium molybdate, 2-4 parts of anhydrous potassium silicate, 2-4 parts of zinc lignosulfonate, 1-3 parts of quick lime, 25-30 parts of dried cow manure or dried sheep manure, 5-10 parts of glucose, 20-30 parts of gamma-polyglutamic acid, 15-20 parts of beef extract peptone agar culture medium, 20-25 parts of trichoderma harzianum, 10-20 parts of rhizobia and 2-4 parts of gamma-aminobutyric acid.
The invention relates to a method for manufacturing a low-yield composite biological organic bacterial fertilizer for cotton fields, which is implemented according to the following steps:
step 1, weighing the following raw materials in parts by weight: 10-20 parts of fulvic acid diamineiron, 5-10 parts of chelated iron, 6-15 parts of calcium ammonium nitrate, 5-10 parts of monocalcium phosphate, 2-4 parts of magnesium nitrate, 2-5 parts of sodium molybdate, 2-4 parts of anhydrous potassium silicate, 2-4 parts of zinc lignosulfonate, 1-3 parts of quick lime, 25-30 parts of dried cow dung or dried sheep dung, 5-10 parts of glucose, 20-30 parts of gamma-polyglutamic acid, 15-20 parts of beef extract peptone agar culture medium, 20-25 parts of trichoderma harzianum, 10-20 parts of rhizobia and 2-4 parts of gamma-aminobutyric acid;
step 2, crushing the fulvic acid diamineiron, chelated iron, calcium ammonium nitrate, monocalcium phosphate, magnesium nitrate, sodium molybdate, anhydrous potassium silicate, zinc lignosulfonate, quick lime, dried cow dung or dried sheep dung weighed in the step 1 by a crusher, sieving by a 100-mesh sieve, stirring, uniformly mixing, standing for 4-5 hours, granulating, drying, sterilizing at 120-200 ℃ for 60-120 minutes, and forming a semi-finished product;
step 3, composting the semi-finished product obtained in the step 2 outdoors, turning the pile for 1-2 times when the central temperature of the pile is not less than 50 ℃ and the pile is full of 7-10 days, airing for 12-48 hours, piling the pile again, and repeatedly airing for 5-7 times and transferring to the environment of 25-30 ℃;
and 4, uniformly spraying a mixed solution (the concentration is less than or equal to 8g/L) prepared by the trichoderma harzianum, rhizobia, glucose, gamma-polyglutamic acid, gamma-aminobutyric acid and a beef extract peptone agar culture medium weighed in the step 1 on the surface of the compost obtained in the step 3, uniformly mixing the mixed solution once every 2-3 hours for 4-5 times, standing for 6-8 hours, bagging for later use when the mass water content of a finished product is less than 5%, and obtaining the low-yield composite biological organic bacterial fertilizer for the cotton field, wherein the effective viable count of the trichoderma harzianum and the rhizobia is more than or equal to 200 hundred million/g, and the prepared composite biological organic bacterial fertilizer for the low-yield cotton field is applied by adopting a spreading mode before spreading, and the application amount is 30-50 kg/mu.
Example 1
Respectively weighing 10 parts of iron diammine fulvate, 5 parts of chelated iron, 6 parts of calcium ammonium nitrate, 5 parts of monocalcium phosphate, 2 parts of magnesium nitrate, 2 parts of sodium molybdate, 2 parts of anhydrous potassium silicate, 2 parts of zinc lignosulfonate, 1 part of quick lime and 25 parts of dry cow dung or dry sheep dung in sequence by weight, crushing, sieving with a 100-mesh sieve, stirring, uniformly mixing, standing for 4 hours, granulating, and drying and sterilizing (120-200 ℃ sterilization for 60-80 minutes) to form a semi-finished product; composting the semi-finished product outdoors, when the temperature in the pile body rises to above 50 ℃, piling for 7 days, turning for 1 time, airing for 12 hours, piling again, when turning for 5 times, transferring to an environment not higher than 25 ℃, weighing 5 parts of glucose, 20 parts of gamma-polyglutamic acid, 15 parts of beef extract peptone agar culture medium, 20 parts of trichoderma harzianum, 10 parts of rhizobium and 2 parts of gamma-aminobutyric acid, preparing a mixed solution (the concentration is less than or equal to 5g/L) by using pure water, uniformly spraying the mixed solution on the surface of the semi-finished product, uniformly mixing once every 2 hours, uniformly mixing for 4 times, standing for 6 hours, and bagging for later use when the mass water content of the finished product is less than 5%.
Example 2
Weighing 12 parts of iron diammine fulvate, 8 parts of chelated iron, 10 parts of calcium ammonium nitrate, 6 parts of monocalcium phosphate, 2 parts of magnesium nitrate, 3 parts of sodium molybdate, 2 parts of anhydrous potassium silicate, 2 parts of zinc lignosulfonate, 2 parts of quick lime and 26 parts of dry cow dung or dry sheep dung in sequence according to the parts by weight, crushing, sieving with a 100-mesh sieve, stirring, uniformly mixing, standing for 5 hours, granulating, and performing drying sterilization (sterilization at the temperature of 120 ℃ and 200 ℃ for 90-110 minutes) to form a semi-finished product; composting the semi-finished product outdoors, when the temperature in the pile body rises to above 50 ℃, fully piling for 8 days, turning for 1 time, airing for 24 hours, piling again, when turning for 6 times, transferring to an environment with the temperature not higher than 30 ℃, weighing 8 parts of glucose, 25 parts of gamma-polyglutamic acid, 18 parts of beef extract peptone agar culture medium, 22 parts of trichoderma harzianum, 12 parts of rhizobium and 4 parts of gamma-aminobutyric acid, preparing a mixed solution (the concentration is less than or equal to 4g/L) by using pure water, uniformly spraying the mixed solution on the surface of the semi-finished product, uniformly mixing once every 3 hours, uniformly mixing for 5 times, standing for 8 hours, and bagging for later use when the mass water content of the finished product is less than 5%.
Example 3
Weighing 17 parts of iron diammine fulvate, 7 parts of chelated iron, 14 parts of calcium ammonium nitrate, 8 parts of monocalcium phosphate, 4 parts of magnesium nitrate, 5 parts of sodium molybdate, 4 parts of anhydrous potassium silicate, 4 parts of zinc lignosulfonate, 3 parts of quick lime and 30 parts of dry cow dung or dry sheep dung in sequence by weight, crushing, sieving by a 100-mesh sieve, stirring, uniformly mixing, standing for 4.5 hours, granulating, and performing drying sterilization (120-200 ℃ sterilization for 85-115 minutes) to form a semi-finished product; and secondly, composting the semi-finished product outdoors, when the temperature in the pile rises to be higher than 50 ℃, piling for 9 days, turning the pile for 1 time, airing for 36 hours, piling the pile again, when the pile is turned and aired for 7 times, transferring the pile to an environment with the temperature of less than or equal to 28 ℃, weighing 10 parts of glucose, 30 parts of gamma-polyglutamic acid, 20 parts of beef extract peptone agar medium, 25 parts of trichoderma harzianum, 20 parts of rhizobia and 3 parts of gamma-aminobutyric acid, preparing a mixed solution (the concentration is less than or equal to 7g/L) by using pure water, uniformly spraying the mixed solution on the surface of the semi-finished product, uniformly mixing the mixed solution once every 2.5 hours, uniformly mixing the mixed solution for 5 times, standing for 7 hours, and bagging the semi-finished product for later use when the mass water content of the finished product is less than 5%.
Example 4
Respectively weighing 20 parts of fulvic acid diamineiron, 9 parts of chelated iron, 13 parts of calcium ammonium nitrate, 9 parts of monocalcium phosphate, 4 parts of magnesium nitrate, 4 parts of sodium molybdate, 3 parts of anhydrous potassium silicate, 3 parts of zinc lignosulfonate, 3 parts of quick lime and 28 parts of dry cow dung or dry sheep dung in sequence by weight, crushing, sieving by a 100-mesh sieve, stirring, uniformly mixing, standing for 4 hours, granulating, and performing drying sterilization (sterilization at the temperature of 120 ℃ and 200 ℃ for 60-100 minutes) to form a semi-finished product; and secondly, composting the semi-finished product outdoors, when the temperature in the pile rises to be higher than 50 ℃, piling the semi-finished product for 10 days, turning the pile for 1 time, airing the pile for 48 hours, piling the pile again, when the pile is turned and aired for 7 times, transferring the pile to an environment with the temperature of less than or equal to 27 ℃, weighing 5 parts of glucose, 25 parts of gamma-polyglutamic acid, 17 parts of beef extract peptone agar culture medium, 23 parts of trichoderma harzianum, 13 parts of rhizobium and 3 parts of gamma-aminobutyric acid, preparing a mixed solution (the concentration is less than or equal to 5.5g/L) by using pure water, uniformly spraying the mixed solution on the surface of the semi-finished product, uniformly mixing the mixed solution once every 2 hours, uniformly mixing the mixed solution for 5 times, standing the mixed solution for 7 hours, and bagging the obtained product for later use when the mass water content of the finished product is less than 5%.
Examples
Experiment 1, the influence of the compound biological organic bacterial fertilizer for the low-yield cotton field on the incidence rate, the yield and the like of cotton verticillium wilt and fusarium wilt in example 4 is as follows:
the test method comprises the following steps:
the research adopts field test, a cotton field with high incidence rate (more than or equal to 60%) of verticillium wilt and blight in nearly three years is selected in shaya town of shaya county in Aksu region of Xinjiang, the salt content of the soil before sowing is 8.8 g/kg, the pH of the soil is 9.32, the cotton field is a low-yield cotton field, the cotton product is medium cotton 35, the irrigation mode is drip irrigation under a film, 2 treatments are set, the blank soil (CK) and the low-yield cotton field are respectively treated by compound biological organic bacterial manure (the using amount is 30 kg/mu), and the test time is 3-10 months in 2019. The compound biological organic bacterial fertilizer for the low-yield cotton fields is used as a base fertilizer and is broadcast for 30-50 kg/mu when soil preparation is carried out before sowing. And measuring the yield of cotton in 10 months and 10 days in the harvesting period, and counting the morbidity of seedling number, cotton boll number of a single plant, verticillium wilt and fusarium wilt, the single boll number and the clothes content.
And (4) analyzing results:
the influence of different test treatments on the indexes of cotton, such as the number of plants per mu, the number of bolls per plant, the number of bolls per mu, verticillium wilt, the incidence rate of blight, the weight of single boll, the clothes rate and the like is shown in a table 1.
TABLE 1 incidence, yield and quality of verticillium wilt and blight of cotton treated by different tests
Note: the same letters after the same column of data in the table indicate that the difference did not reach the significance level (p < 0.05).
Table 2 shows that: the number of single plants treated by the compound biological organic bacterial manure for the low-yield cotton fields is increased by 1.23, so that the significant difference (p is less than 0.05) is achieved; the morbidity of the verticillium wilt and the fusarium wilt is reduced by 63 percent compared with the control treatment, the number of plants per mu is greatly increased by 13.08 percent compared with the control treatment, and the increase rate is 13.05 percent (p is less than 0.05); the treatment yield is 388.76 kg/mu and 446.52 kg/mu respectively, and the yield of the low-yield cotton field treated by the compound biological organic bacterial manure is increased by 17 percent (p is less than 0.05) compared with the contrast seed cotton.
And (4) conclusion:
the composite biological organic bacterial fertilizer for the low-yield cotton field effectively improves the verticillium wilt and blight resistance of cotton and promotes the seedling number, the boll number and the boll weight of the cotton.
Examples
Experiment 2, the composite biological organic bacterial fertilizer for low-yield cotton fields in example 2 has the following effects on the volume weight, the water content, the saline-alkali degree and the nutrient content of soil:
the test method comprises the following steps:
the research adopts a field test, a cotton field with high incidence rate (more than or equal to 60%) of verticillium wilt and blight in nearly three years is selected in the shaya town of shaya county of Aksu region of Xinjiang, the salt content of the soil before sowing is 8.8 g/kg, the pH value of the soil is 9.32, the planted crop is medium cotton 35, the irrigation mode is drip irrigation under a film, 2 treatments are set, respectively blank soil (CK), the low-yield cotton field is treated by compound biological organic bacterial manure, and the test time is 3-10 months in 2019 years. The compound biological organic bacterial fertilizer for the low-yield cotton fields is used as a base fertilizer and is applied by spreading 30-50kg per mu when soil preparation is carried out before sowing. Collecting soil with 0-30cm and 30-60cm of root layer of cotton field, air drying, and detecting volume weight, water content, total salt, pH, total nitrogen, total phosphorus and total potassium content.
And (4) analyzing results:
the treatment of the compound biological organic bacterial fertilizer for the low-yield cotton fields has certain influence on the volume weight, the water content, the saline-alkali degree and the nutrient content of soil, and the result shows that: after the compound biological organic bacterial manure is applied, the volume weight, total salt and pH of soil can be obviously reduced, and the reduction range is different from 10-50%; can increase the water content, total nitrogen, total phosphorus and total potassium content (p is less than 0.05) of the soil, and the amplification is more than 15-50% (see table 2).
TABLE 2 influence of different experimental treatments on soil volume weight, water content, salinity and alkalinity and nutrient content
Note: the same letters after the same column of data in the table indicate that the difference did not reach a significance level (p < 0.05).
And (4) conclusion:
the compound biological organic bacterial fertilizer for the low-yield cotton fields of 30-50 kg/mu is used for treatment, and the compound biological organic bacterial fertilizer is applied in a broadcasting and dressing mode before sowing, so that the saline-alkali content of the soil can be obviously reduced, and the soil fertility (p is less than 0.05) can be obviously improved.
Claims (8)
1. The compound biological organic bacterial fertilizer for the low-yield cotton fields is characterized by comprising the following raw materials in parts by weight: 10-20 parts of fulvic acid diamineiron, 5-10 parts of chelated iron, 6-15 parts of calcium ammonium nitrate, 5-10 parts of monocalcium phosphate, 2-4 parts of magnesium nitrate, 2-5 parts of sodium molybdate, 2-4 parts of anhydrous potassium silicate, 2-4 parts of zinc lignosulfonate, 1-3 parts of quick lime, 25-30 parts of dried cow manure or dried sheep manure, 5-10 parts of glucose, 20-30 parts of gamma-polyglutamic acid, 15-20 parts of beef extract peptone agar culture medium, 20-25 parts of trichoderma harzianum, 10-20 parts of rhizobia and 2-4 parts of gamma-aminobutyric acid.
2. The compound bio-organic bacterial fertilizer for the low-yield cotton fields as claimed in claim 1, wherein the effective viable count of trichoderma harzianum and rhizobia is more than or equal to 200 hundred million/g.
3. A method for manufacturing a compound biological organic bacterial fertilizer for a low-yield cotton field is characterized by comprising the following steps:
step 1, weighing the following raw materials in parts by weight: 10-20 parts of fulvic acid diamineiron, 5-10 parts of chelated iron, 6-15 parts of calcium ammonium nitrate, 5-10 parts of monocalcium phosphate, 2-4 parts of magnesium nitrate, 2-5 parts of sodium molybdate, 2-4 parts of anhydrous potassium silicate, 2-4 parts of zinc lignosulfonate, 1-3 parts of quick lime, 25-30 parts of dried cow dung or dried sheep dung, 5-10 parts of glucose, 20-30 parts of gamma-polyglutamic acid, 15-20 parts of beef extract peptone agar culture medium, 20-25 parts of trichoderma harzianum, 10-20 parts of rhizobia and 2-4 parts of gamma-aminobutyric acid;
step 2, crushing, stirring, uniformly mixing, standing for 4-5 hours, granulating, drying and sterilizing the crushed materials to form a semi-finished product;
step 3, composting the semi-finished product obtained in the step 2 outdoors;
and 4, preparing the glucose, the gamma-polyglutamic acid, the trichoderma harzianum, the rhizobia and the gamma-aminobutyric acid weighed in the step 1 into a mixed solution with the concentration of less than or equal to 8g/L by using pure water, uniformly spraying the mixed solution on the surface of the compost obtained in the step 3, uniformly mixing the mixed solution once every 2 to 3 hours, uniformly mixing the mixed solution for 4 to 5 times, standing the mixed solution for 6 to 8 hours, bagging the mixed solution for later use when the mass water content of a finished product is less than 5 percent, and obtaining the composite biological organic bacterial fertilizer for the low-yield cotton fields, wherein the effective viable count of the trichoderma harzianum and the rhizobia is more than or equal to 200 billion/g.
4. The method for preparing the compound bio-organic bacterial fertilizer for the low-yield cotton fields as claimed in claim 3, wherein the step 2 is implemented by crushing the raw materials by a crusher and then sieving the crushed raw materials by a 100-mesh sieve.
5. The method for preparing the composite bio-organic bacterial fertilizer for the low-yield cotton fields as claimed in claim 4, wherein the sterilization in the step 2 is performed at 120-200 ℃ for 60-120 minutes.
6. The method for manufacturing the compound bio-organic bacterial manure for the low-yield cotton fields as claimed in claim 5, wherein the compost in the step 3 is specifically as follows: composting the semi-finished product outdoors, turning the pile for 1 time when the central temperature of the pile is not less than 50 ℃ and the pile is full for 7-10 days, airing for 12-48 hours, piling the pile again, airing for 5-7 times repeatedly, and transferring to the environment of 25-30 ℃.
7. The method for preparing the composite biological organic bacterial fertilizer for the low-yield cotton fields according to claim 6, wherein the prepared composite biological organic bacterial fertilizer for the low-yield cotton fields is applied by a spreading way when soil preparation is carried out before sowing.
8. The method for preparing the compound biological organic bacterial fertilizer for the low-yield cotton fields as claimed in claim 7, wherein the application amount of the compound biological organic bacterial fertilizer for the low-yield cotton fields is 30-50 kg/mu.
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CN108610183A (en) * | 2018-07-11 | 2018-10-02 | 新疆农垦科学院 | Prevent the fertilizer of cotton verticillium wilt |
CN110846243A (en) * | 2019-09-29 | 2020-02-28 | 石河子大学 | Biocontrol compound microbial inoculum and preparation method and application thereof |
CN111747792A (en) * | 2020-07-28 | 2020-10-09 | 轩凯生物科技(山东)有限公司 | Microbial source combined biological stimulator and application thereof |
CN113563892A (en) * | 2021-07-28 | 2021-10-29 | 涿州拜奥威生物科技有限公司 | Soil permeation water-retaining agent composition |
CN113831182A (en) * | 2021-09-30 | 2021-12-24 | 海南金雨丰生物工程有限公司 | Trichoderma harzianum compound bacterial fertilizer and preparation method and application thereof |
CN113968762A (en) * | 2021-12-01 | 2022-01-25 | 中化农业(临沂)研发中心有限公司 | Special cotton fertilizer suitable for saline-alkali soil and preparation method and application thereof |
CN114716279A (en) * | 2022-02-16 | 2022-07-08 | 操苏立 | Water-soluble compound fertilizer special for cotton and preparation method thereof |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN108610183A (en) * | 2018-07-11 | 2018-10-02 | 新疆农垦科学院 | Prevent the fertilizer of cotton verticillium wilt |
CN110846243A (en) * | 2019-09-29 | 2020-02-28 | 石河子大学 | Biocontrol compound microbial inoculum and preparation method and application thereof |
CN111747792A (en) * | 2020-07-28 | 2020-10-09 | 轩凯生物科技(山东)有限公司 | Microbial source combined biological stimulator and application thereof |
CN113563892A (en) * | 2021-07-28 | 2021-10-29 | 涿州拜奥威生物科技有限公司 | Soil permeation water-retaining agent composition |
CN113831182A (en) * | 2021-09-30 | 2021-12-24 | 海南金雨丰生物工程有限公司 | Trichoderma harzianum compound bacterial fertilizer and preparation method and application thereof |
CN113968762A (en) * | 2021-12-01 | 2022-01-25 | 中化农业(临沂)研发中心有限公司 | Special cotton fertilizer suitable for saline-alkali soil and preparation method and application thereof |
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