CN114455710A - A method for plant screening and community construction suitable for urban landscape water restoration - Google Patents

A method for plant screening and community construction suitable for urban landscape water restoration Download PDF

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CN114455710A
CN114455710A CN202210109124.8A CN202210109124A CN114455710A CN 114455710 A CN114455710 A CN 114455710A CN 202210109124 A CN202210109124 A CN 202210109124A CN 114455710 A CN114455710 A CN 114455710A
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plant
water
phosphorus
nitrogen
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崔姗姗
陈�全
陈湘
戴璨
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Hubei University
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Abstract

本发明涉及一种适用于城市景观水体修复的植物筛选与群落构建方法,其特征在于,收集水葱、香蒲、鸢尾、菖蒲、美人蕉、再力花的繁殖材料,按如下方式种植于装载有人工湿地基质的收纳盒内:1)每种植物单独种植于收纳盒内;2)将生长型相同的植物组合种植于收纳盒内;包括以下步骤:1)定期添加营养;2)监测植物生长和水质指标;3)根据植物的生长状况以及水体内氮磷元素的移除效果判断不同植物物种的竞争力强弱,从而进行筛选。将六种植物混合种植于收纳盒内,构建群落,通过不同植物之间的生态位差异,以期弱化种间竞争、促进植物对于富营养化水体中氮磷的吸收与利用。The invention relates to a method for plant screening and community construction suitable for urban landscape water restoration. In the storage box of the wetland matrix: 1) each plant is planted in the storage box alone; 2) the plants with the same growth type are combined and planted in the storage box; including the following steps: 1) regularly add nutrients; 2) monitor plant growth and Water quality index; 3) Judging the competitiveness of different plant species according to the growth status of plants and the removal effect of nitrogen and phosphorus elements in the water, so as to screen. Six kinds of plants were mixed and planted in the storage box to build a community, and through the niche differences between different plants, in order to weaken the competition between species and promote the absorption and utilization of nitrogen and phosphorus in eutrophic water by plants.

Description

一种适用于城市景观水体修复的植物筛选与群落构建方法A method for plant screening and community construction suitable for urban landscape water restoration

技术领域technical field

本发明涉及富营养化水体修复领域,尤其涉及一种适用于城市景观水体修复的植物筛选与群落构建方法。The invention relates to the field of eutrophication water body restoration, in particular to a plant screening and community construction method suitable for urban landscape water body restoration.

背景技术Background technique

城市景观水体作为城市水生态环境的重要组成部分,能够优化生活环境,与人们的生活质量息息相关。相比于湖泊河流等自然水体,城市景观水体大多数为封闭式水体,具有水域面积小、流动性差等特点,生态系统结构与功能较为简单,自净能力与抵抗外界干扰能力较弱。随着工业的发展与人口密度的增加,汽车尾气中含有的大量NO、NO2等气体与地表营养随着雨水与地表径流进入城市景观水体,此外还包括生活垃圾的侵入,这些外来营养的输入导致水体中的氮磷浓度过高,造成水体富营养化,出现藻类与浮游生物大量繁殖,水中溶解氧下降,水体发臭等现象,严重影响居民的生活质量,丧失作为景观水体的观赏价值。As an important part of urban water ecological environment, urban landscape water can optimize the living environment and is closely related to people's quality of life. Compared with natural water bodies such as lakes and rivers, most urban landscape water bodies are closed water bodies, which have the characteristics of small water area and poor mobility. With the development of industry and the increase of population density, a large amount of NO, NO 2 and other gases and surface nutrients contained in automobile exhaust enter urban landscape water bodies along with rainwater and surface runoff, and also include the intrusion of domestic garbage, the input of these foreign nutrients As a result, the concentration of nitrogen and phosphorus in the water body is too high, resulting in eutrophication of the water body, the occurrence of algae and plankton blooms, the decrease of dissolved oxygen in the water, and the odor of the water body, which seriously affects the quality of life of residents and loses its ornamental value as a landscape water body.

在众多治理城市景观水体富营养化的方法中,传统的物理方法如曝气、打捞成本过高,且治标不治本。化学方法虽然效果显著,但易造成二次污染。而生物法中的植物修复通过水生植物的吸收同化以及与微生物的协同作用去除水体中氮磷,具有低成本、低能耗、高效、可自我维持更新、运行维护方便等特点,同时还能美化环境,被广泛应用于城市景观水体的生态修复。Among the many methods to control the eutrophication of urban landscape water bodies, the traditional physical methods such as aeration and salvage are too expensive, and they can only cure the symptoms but not the root causes. Although the chemical method is effective, it is easy to cause secondary pollution. The phytoremediation in the biological method removes nitrogen and phosphorus in the water body through the absorption and assimilation of aquatic plants and the synergy with microorganisms. , is widely used in the ecological restoration of urban landscape water bodies.

水生植物种类间生长特性不同,对水体中营养元素的耐受程度与吸收利用能力存在差异,因此水生植物的选择在很大程度上影响水体的净化效果。同时,植物的组合也会影响氮磷的去除效果。一方面,植物组合能导致种间竞争,竞争能力强的物种会抑制其他物种的生长,获取更多的资源满足生长繁殖,竞争能力弱的物种则因获取的资源较少,快速或逐渐地消亡。特别是在生态位相似的物种之间,竞争作用(特别是化感作用)影响植物的存活与生物量的积累,如若大量种植某些竞争能力较弱的物种还会造成植物的大批量死亡,从而人为增加水体内外源性营养物的输入。另一方面,植物的组合能提升群落的物种多样性,也有研究表明物种多样性会造成生态位互补,提升群落水平吸收氮磷的能力,从而优化水体净化的效果。生态位互补是指由于物种间存在资源需求与栖息地利用上的差异,导致不同植物之间出现资源分配与生态位分化,降低不同种类植物在空间、时间以及营养元素种类、形式与数量上的竞争强度,促进植物之间的共存与群落的稳定,进而提升群落的初级生产力和资源的利用效率。因此,在水体修复的实际应用中,应该综合考虑物种的竞争能力以及构建群落的水生植物之间在生态位上的分化程度。The growth characteristics of aquatic plants are different, and there are differences in the tolerance and absorption and utilization capacity of nutrient elements in the water body. Therefore, the selection of aquatic plants greatly affects the purification effect of the water body. At the same time, the combination of plants will also affect the removal of nitrogen and phosphorus. On the one hand, plant combinations can lead to interspecific competition. Species with strong competitiveness will inhibit the growth of other species and obtain more resources to meet growth and reproduction. Species with weak competitiveness will quickly or gradually die out due to the acquisition of fewer resources. . Especially among species with similar ecological niches, competition (especially allelopathy) affects the survival of plants and the accumulation of biomass. If a large number of species with weaker competition ability are planted in large quantities, they will also cause mass death of plants. Thereby artificially increasing the input of exogenous nutrients in the water body. On the other hand, the combination of plants can improve the species diversity of the community, and some studies have shown that species diversity can lead to niche complementarity and improve the ability of the community to absorb nitrogen and phosphorus, thereby optimizing the effect of water purification. Niche complementarity means that due to differences in resource requirements and habitat utilization among species, resource allocation and niche differentiation among different plants occur, reducing the spatial, temporal, and nutrient types, forms and quantities of different plants. The competition intensity promotes the coexistence between plants and the stability of the community, thereby improving the primary productivity of the community and the utilization efficiency of resources. Therefore, in the practical application of water body remediation, the competition ability of species and the degree of niche differentiation among aquatic plants that build the community should be comprehensively considered.

尽管群落的物种多样性有潜力提升其对于氮磷的吸收,但目前关于水生植物净化水体的应用多默认沉水植物与其他生活型的组合优势,既无对物种竞争能力的评价,也不适合城市内较浅的水体和观赏需求。相比于沉水植物,挺水植物因其生物量大、适应性强,能大量去除水体中的氮磷,兼具较高的观赏价值,同时方便通过后期的人工收割将其同化的氮磷从系统中转移,常常被用于景观水体或者富营养化程度高的水体的修复。本发明选择六种具观赏性的挺水植物,从植物的种间竞争作用与资源利用效率出发,为城市景观水体修复的植物筛选与群落构建提供了一种应用范式。Although the species diversity of the community has the potential to improve its absorption of nitrogen and phosphorus, the current application of aquatic plants to purify water mostly defaults to the combined advantages of submerged plants and other life forms. Shallow water bodies and viewing needs within the city. Compared with submerged plants, emerging plants can remove a large amount of nitrogen and phosphorus in water because of their large biomass and strong adaptability, and have high ornamental value. Transfer from the system is often used for the restoration of landscape water bodies or water bodies with a high degree of eutrophication. The invention selects six ornamental emergent plants, and provides an application paradigm for plant screening and community construction of urban landscape water body restoration based on the interspecific competition effect and resource utilization efficiency of plants.

发明内容SUMMARY OF THE INVENTION

本发明所要解决的技术问题是提供一种适用于城市景观水体修复的植物筛选与群落构建方法,其目的是在构建植物群落进行城市景观水体生态修复时,为植物的筛选与配置提供实现方案。The technical problem to be solved by the present invention is to provide a plant screening and community construction method suitable for urban landscape water body restoration, the purpose of which is to provide a realization scheme for plant selection and configuration when constructing a plant community for urban landscape water body ecological restoration.

本发明解决上述技术问题的技术方案如下:一种适用于城市景观水体修复的植物筛选方法,其特征在于,收集观赏性挺水植物水葱、香蒲、鸢尾、菖蒲、美人蕉、再力花的繁殖材料,分别按以下方式种植培育于装载有人工湿地基质的收纳盒内:The technical scheme of the present invention to solve the above-mentioned technical problems is as follows: a plant screening method suitable for urban landscape water restoration, characterized in that the reproduction of ornamental emergent plants scallion, cattail, iris, calamus, canna, and reliflower is collected. The materials are planted and cultivated in the storage box loaded with the constructed wetland matrix in the following ways:

1)每种植物单独种植于收纳盒内;1) Each plant is planted in the storage box separately;

2)将生长型相同的植物组合种植于收纳盒内;2) Plant the same plant combination in the growth type in the storage box;

包括以下步骤:Include the following steps:

1)定期添加营养;1) Regularly add nutrition;

2)监测植物生长情况以及水质指标;2) Monitor plant growth and water quality indicators;

3)根据植物的生长状况以及水体内氮磷元素的移除效果判断不同植物物种的竞争力强弱,从而进行筛选。3) Judging the competitiveness of different plant species according to the growth status of plants and the removal effect of nitrogen and phosphorus elements in the water, so as to screen.

人工湿地基质主要由河沙构成,将河沙用自来水冲洗5-6遍,直至出水澄清,将清洗后的泥沙用0.2mol/L的稀盐酸浸泡1-2天,然后依次用自来水与蒸馏水冲洗,去除泥沙中的可溶性营养物质,以防止泥沙中原有的氮磷影响测量结果。The constructed wetland matrix is mainly composed of river sand. Rinse the river sand with tap water for 5-6 times until the effluent is clear. Soak the cleaned sediment with 0.2mol/L dilute hydrochloric acid for 1-2 days, and then use tap water and distilled water in turn. Flush to remove soluble nutrients in the sediment to prevent the original nitrogen and phosphorus in the sediment from affecting the measurement results.

收纳箱的尺寸规格为长63cm×宽45cm×高39cm,每个收纳箱均种植12株植物,故植物栽种的密度为43.5株/m2,植物的根茎固定在8cm深的泥沙中,培养期间水位维持在14cm-18cm之间。The size of the storage box is 63cm long x 45cm wide x 39cm high, and each storage box is planted with 12 plants, so the planting density is 43.5 plants/m 2 , and the rhizomes of the plants are fixed in 8cm deep sand and cultivated During this period, the water level was maintained between 14cm-18cm.

植物的生长环境为温室静水条件,培养水体的初始状态为10%的霍格兰营养液,其总氮浓度为12mg/L,总磷浓度为4mg/L,体积为30L;在植物培养的两个月期间,每5天进行营养液的添加,使用氯化铵(NH4CL)、磷酸二氢钾(KH2PO4)作为营养液中氮磷的成分,每次添加的营养液中氮总量为70mg,磷总量为10mg,对于不同的植物组合,采取定量添加氮磷并用蒸馏水定容至统一水位的原则。The growth environment of the plants is the still water condition of the greenhouse, the initial state of the culture water is 10% Hoagland nutrient solution, the total nitrogen concentration is 12mg/L, the total phosphorus concentration is 4mg/L, and the volume is 30L; During the month, the nutrient solution was added every 5 days, and ammonium chloride (NH 4 CL) and potassium dihydrogen phosphate (KH 2 PO4) were used as the components of nitrogen and phosphorus in the nutrient solution. The amount is 70mg, and the total amount of phosphorus is 10mg. For different plant combinations, the principle of quantitatively adding nitrogen and phosphorus and distilling the volume to a uniform water level with distilled water is adopted.

培养箱置于温室大棚内进行培养,8:00-20:00的时间段温度设置为30℃,20:00-8:00的时间段温度设置为25℃,湿度控制在50%;在8:00-20:00的时间段使用钠灯对植物进行光照补充,培养期间保持良好通风。The incubator was placed in a greenhouse for cultivation. The temperature was set to 30 °C during the period of 8:00-20:00, and the temperature was set to 25 °C during the period of 20:00-8:00, and the humidity was controlled at 50%; at 8 During the period of :00-20:00, the plants were supplemented with sodium lamps, and good ventilation was maintained during the cultivation period.

一种适用于城市景观水体修复的植物群落构建方法,其特征在于,收集观赏性挺水植物水葱、香蒲、鸢尾、菖蒲、美人蕉、再力花的繁殖材料,并混合种植培育于装载有人工湿地基质的收纳盒内,构建群落,通过不同植物之间的生态位差异,以期弱化种间竞争、促进植物对于富营养化水体中氮磷的吸收与利用。A method for constructing a plant community suitable for urban landscape water restoration, characterized in that propagating materials of ornamental emergent plants scallion, cattail, iris, calamus, canna, and jasperia are collected, and mixed planting and cultivating in artificial plants loaded with artificial In the storage box of the wetland matrix, a community is constructed, and the ecological niche difference between different plants is used to weaken the competition among species and promote the absorption and utilization of nitrogen and phosphorus in eutrophic water by plants.

有益效果:将生长型相同的植物进行组合种植,在生态位相似的情况下,比较植物的生长状况,从中筛选出竞争能力更强的植物。同时将不同生长型的植物进行搭配,形成植物群落间的生态位差异,造成不同种类植物在资源分配与利用上的生态位互补,从而促进群落对于氮磷的吸收去除。从植物的种间竞争关系与资源利用效率出发,分别为水体生态修复技术中植物的筛选与群落构建提供了方案:(1)同一生长型的植物进行组合种植,判断其竞争力强弱,从而进行植物的筛选;(2)使用不同生长型的植物构建群落,通过它们之间的生态位差异,提升群落的稳定性以及水体修复效果。这两种方案在实际应用中可以联合使用,首先在同一生长型的植物之间进行筛选,然后利用筛选出的植物搭配不同生长型进行群落构建,在最大程度上促进群落的稳定、景观的多样、同时提升水体修复效果。Beneficial effect: Plants with the same growth type are planted in combination, and in the case of similar ecological niches, the growth conditions of the plants are compared, and the plants with stronger competitiveness are screened out. At the same time, plants of different growth types are matched to form niche differences between plant communities, resulting in complementary niches of different types of plants in resource allocation and utilization, thereby promoting the absorption and removal of nitrogen and phosphorus by the community. Starting from the interspecific competition relationship and resource utilization efficiency of plants, we provide solutions for plant selection and community construction in water ecological restoration technology: (1) Plants of the same growth type are combined to determine their competitiveness, so as to determine their competitiveness. Screening of plants; (2) Use plants of different growth types to build communities, and improve the stability of the community and the effect of water restoration through the differences in their niches. These two schemes can be used in combination in practical applications. First, the plants of the same growth type are screened, and then the selected plants are matched with different growth types to construct the community, which can promote the stability of the community and the diversity of the landscape to the greatest extent. , At the same time improve the water body repair effect.

具体实施方式Detailed ways

以下对本发明的原理和特征进行描述,所举实例只用于解释本发明,并非用于限定本发明的范围。The principles and features of the present invention are described below, and the examples are only used to explain the present invention, but not to limit the scope of the present invention.

一种适用于城市景观水体修复的植物筛选方法,其特征在于,收集观赏性挺水植物水葱、香蒲、鸢尾、菖蒲、美人蕉、再力花的繁殖材料,分别按以下方式种植培育于装载有人工湿地基质的收纳盒内:A plant screening method suitable for the restoration of urban landscape water bodies, characterized in that the propagating materials of the ornamental emergent plants scallion, cattail, iris, calamus, canna, and Zaili flower are collected, and are planted and cultivated in the following manners respectively. In the storage box of the constructed wetland matrix:

1)每种植物单独种植于收纳盒内;1) Each plant is planted in the storage box separately;

2)将生长型相同的植物组合种植于收纳盒内;2) Plant the same plant combination in the growth type in the storage box;

包括以下步骤:Include the following steps:

1)定期添加营养;1) Regularly add nutrition;

2)监测植物生长情况以及水质指标;2) Monitor plant growth and water quality indicators;

3)根据植物的生长状况以及水体内氮磷元素的移除效果判断不同植物物种的竞争力强弱,从而进行筛选。3) Judging the competitiveness of different plant species according to the growth status of plants and the removal effect of nitrogen and phosphorus elements in the water, so as to screen.

人工湿地基质主要由河沙构成,将河沙用自来水冲洗5-6遍,直至出水澄清,将清洗后的泥沙用0.2mol/L的稀盐酸浸泡1-2天,然后依次用自来水与蒸馏水冲洗,去除泥沙中的可溶性营养物质,以防止泥沙中原有的氮磷影响测量结果。The constructed wetland matrix is mainly composed of river sand. Rinse the river sand with tap water for 5-6 times until the effluent is clear. Soak the cleaned sediment with 0.2mol/L dilute hydrochloric acid for 1-2 days, and then use tap water and distilled water in turn. Flush to remove soluble nutrients in the sediment to prevent the original nitrogen and phosphorus in the sediment from affecting the measurement results.

收纳箱的尺寸规格为长63cm×宽45cm×高39cm,每个收纳箱均种植12株植物,故植物栽种的密度为43.5株/m2,植物的根茎固定在8cm深的泥沙中,培养期间水位维持在14cm-18cm之间。The size of the storage box is 63cm long x 45cm wide x 39cm high, and each storage box is planted with 12 plants, so the planting density is 43.5 plants/m 2 , and the rhizomes of the plants are fixed in 8cm deep sand and cultivated During this period, the water level was maintained between 14cm-18cm.

植物的生长环境为温室静水条件,培养水体的初始状态为10%的霍格兰营养液,其总氮浓度为12mg/L,总磷浓度为4mg/L,体积为30L;在植物培养的两个月期间,每5天进行营养液的添加,使用氯化铵(NH4CL)、磷酸二氢钾(KH2PO4)作为营养液中氮磷的成分,每次添加的营养液中氮总量为70mg,磷总量为10mg,对于不同的植物组合,采取定量添加氮磷并用蒸馏水定容至统一水位的原则。The growth environment of the plants is the still water condition of the greenhouse, the initial state of the culture water is 10% Hoagland nutrient solution, the total nitrogen concentration is 12mg/L, the total phosphorus concentration is 4mg/L, and the volume is 30L; During the month, the nutrient solution was added every 5 days, and ammonium chloride (NH 4 CL) and potassium dihydrogen phosphate (KH 2 PO4) were used as the components of nitrogen and phosphorus in the nutrient solution. The amount is 70mg, and the total amount of phosphorus is 10mg. For different plant combinations, the principle of quantitatively adding nitrogen and phosphorus and making up the volume with distilled water to a uniform water level is adopted.

培养箱置于温室大棚内进行培养,8:00-20:00的时间段温度设置为30℃,20:00-8:00的时间段温度设置为25℃,湿度控制在50%;在8:00-20:00的时间段使用钠灯对植物进行光照补充,培养期间保持良好通风。The incubator was placed in a greenhouse for cultivation. The temperature was set to 30 °C during the period of 8:00-20:00, and the temperature was set to 25 °C during the period of 20:00-8:00, and the humidity was controlled at 50%; at 8 During the period of :00-20:00, the plants were supplemented with sodium lamps, and good ventilation was maintained during the cultivation period.

一种适用于城市景观水体修复的植物群落构建方法,其特征在于,收集观赏性挺水植物水葱、香蒲、鸢尾、菖蒲、美人蕉、再力花的繁殖材料,并混合种植培育于装载有人工湿地基质的收纳盒内,构建群落,通过不同植物之间的生态位差异,以期弱化种间竞争、促进植物对于富营养化水体中氮磷的吸收与利用。A method for constructing a plant community suitable for urban landscape water restoration, characterized in that propagating materials of ornamental emergent plants scallion, cattail, iris, calamus, canna, and jasperia are collected, and mixed planting and cultivating in artificial plants loaded with artificial In the storage box of the wetland matrix, a community is constructed, and the ecological niche difference between different plants is used to weaken the competition among species and promote the absorption and utilization of nitrogen and phosphorus in eutrophic water by plants.

具体实验步骤如下:The specific experimental steps are as follows:

本发明选取的六种常见挺水植物水葱(Schoenoplectus tabernaemontani)、香蒲(Typha orientalis)、鸢尾(Iris tectorum)、菖蒲(Acorus calamus)、美人蕉(Cannaindica)与再力花(Thalia dealbata)来自同一苗圃基地。选择大小适中、体型相近的植株个体,依据不同类别植物的生长型,分别将其修剪至相近的高度,用蒸馏水清洗植物根部与叶片,去掉残留的氮磷等营养物质,进行称量并记录其鲜重。同时记录美人蕉、再力花、菖蒲、鸢尾的叶片数量,水葱、香蒲的分株数量。挑选剩下的植株于室外培养,用于对死亡植物的补充。选取长63cm×宽45cm×高39cm的透明收纳盒作为植物生长的容器,收纳盒内铺设8cm厚的沙层,用于植物的固定,每个收纳箱种植12棵植物,栽种密度为43.5株/m2。为防止泥沙中原有的氮磷影响测量结果,前期将河沙用自来水冲洗5-6遍,直至出水澄清,将清洗后的泥沙用0.2mol/L的稀盐酸浸泡1-2天,去除泥沙中的可溶性营养物质,然后依次用自来水与蒸馏水冲洗。Six common emergent plants, Schoenoplectus tabernaemontani, Typha orientalis, Iris tectorum, Acorus calamus, Cannaindica and Thalia dealbata, are selected in the present invention from the same nursery. base. Select individual plants with moderate size and similar body shape. According to the growth type of different types of plants, trim them to similar heights. Wash the roots and leaves of the plants with distilled water to remove residual nitrogen and phosphorus and other nutrients, weigh and record them. Fresh weight. At the same time, the number of leaves of canna, Zailihua, calamus and iris, and the number of ramets of water onion and cattail were recorded. The remaining plants were selected and cultivated outdoors to supplement the dead plants. A transparent storage box of length 63cm x width 45cm x height 39cm was selected as a container for plant growth, and an 8cm thick sand layer was laid in the storage box to fix the plants. Each storage box was planted with 12 plants, and the planting density was 43.5 plants/ m 2 . In order to prevent the original nitrogen and phosphorus in the sediment from affecting the measurement results, the river sand was washed with tap water 5-6 times in the early stage until the water was clear, and the washed sediment was soaked in 0.2mol/L dilute hydrochloric acid for 1-2 days to remove Soluble nutrients in the sediment, and then rinsed with tap water and distilled water in turn.

本发明共设置了三种种植方式,分别为:单一种植,双物种组合以及六种植物混种。不同方式下植物的配置结果如下:在单一种植中,每种植物3个重复,共计18个收纳箱。在双物种组合中,分别将大型(再力花与美人蕉),瘦长型(水葱与香蒲),扇型(水鸢尾与水菖蒲)三种生长型相同的植物进行组合种植,每种组合6个重复,单个重复中,每种植物各6株,共计18个收纳箱。在六种植物混种中,每种植物种植2株,共计6种12株植物,设置18个收纳箱的重复。因此,三种种植方式中,每种植物的重复数量均为36株,收纳箱共计54个。The present invention provides three planting modes, which are: single planting, two-species combination and six-plant mixed planting. The configuration results of plants in different ways are as follows: In monoculture, each plant was replicated 3 times, totaling 18 storage boxes. In the two-species combination, three types of plants with the same growth types were planted in combination: large (Zarya and Canna), slender (water onion and cattail), and fan-shaped (water iris and water calamus), each combination of 6 In a single repetition, there are 6 plants of each type, a total of 18 storage boxes. Among the six plant mixtures, 2 of each plant were planted, for a total of 12 plants of 6 species, and 18 repetitions of storage boxes were set up. Therefore, in the three planting methods, the number of repetitions of each plant is 36, and there are 54 storage boxes in total.

根据不同的搭配组合,将带有编号的植株均匀地种植于收纳箱的随机位置,并将收纳箱随机摆放于温室中的四个苗床,加入30L的10%霍格兰营养液,进行刻度线标记。初始培养水体的总氮浓度为12mg/L,总磷浓度为4mg/L,营养物的浓度高于地表水V类的标准。以沙层为起点,测量并记录植物的初始株高。温度设置为白天(8:00-20:00)30℃,夜晚(20:00-8:00)25℃,湿度控制在50%,在白天阶段使用钠灯对植物进行光照补充。培养期间保持良好通风,水位保持在14cm-18cm之间。According to different combinations, the numbered plants were evenly planted in random positions of the storage box, and the storage boxes were randomly placed on the four seedbeds in the greenhouse, and 30L of 10% Hoagland nutrient solution was added for calibration. line markers. The total nitrogen concentration of the initial culture water was 12 mg/L, the total phosphorus concentration was 4 mg/L, and the nutrient concentration was higher than the standard of surface water category V. Using the sand layer as a starting point, measure and record the initial plant height. The temperature was set to 30°C during the day (8:00-20:00), 25°C at night (20:00-8:00), the humidity was controlled at 50%, and the plants were supplemented with sodium lamps during the day. Maintain good ventilation during cultivation and keep the water level between 14cm-18cm.

在培养期间中对所有的收纳箱额外添加氮磷。使用氯化铵(NH4CL)、磷酸二氢钾(KH2PO4)作为营养液中氮磷的成分,每五天对所有的收纳箱添加等量相同浓度的营养液,添加量为总氮70mg,总磷10mg,添加完后用蒸馏水定容至30L对应的刻度线,搅拌均匀后,在收纳箱四个角落及中央各取20ml水样,共100ml水样混合均匀。取完水样后,当天测量其总氮总磷的浓度,总氮的检测方法为过硫酸钾紫外分光光度法,总磷的检测方法为钼锑抗分光光度法,具体参照《水和废水监测分析方法(第四版)》。Nitrogen and phosphorus were additionally added to all storage boxes during the incubation period. Ammonium chloride (NH 4 CL) and potassium dihydrogen phosphate (KH 2 PO4) were used as the nitrogen and phosphorus components in the nutrient solution, and the same amount of nutrient solution with the same concentration was added to all storage boxes every five days, and the added amount was total nitrogen 70mg, total phosphorus 10mg, after adding, dilute the volume to the corresponding 30L mark with distilled water, stir well, take 20ml water samples from each of the four corners and the center of the storage box, and mix a total of 100ml water samples evenly. After taking the water samples, measure the concentration of total nitrogen and total phosphorus on the same day. The detection method of total nitrogen is potassium persulfate ultraviolet spectrophotometry, and the detection method of total phosphorus is molybdenum antimony anti-spectrophotometry. Methods of Analysis (Fourth Edition).

培养两个月后,收获所有的植物并将根部的泥沙清理干净,记录开花个体与死亡个体,同时对植物的形态指标进行测量。测量指标包括:1株高2鲜重3叶片数量4分株数量,六种植物的生长形态不同,测量指标存在差异。美人蕉、再力花、菖蒲与鸢尾测量的为叶片数量,香蒲、水葱与美人蕉统计其分株数量。After two months of cultivation, all plants were harvested and the roots were cleaned of sediment, flowering individuals and dead individuals were recorded, and the morphological indicators of the plants were measured. The measurement indicators include: 1 plant height, 2 fresh weight, 3 number of leaves, and 4 number of ramets. The six kinds of plants have different growth patterns, and there are differences in measurement indicators. The number of leaves was measured for Canna, Zailihua, Iris and Iris, and the number of ramets was counted for Cattail, Scallion and Canna.

在经过两个月的培养后,其结果如下:After two months of cultivation, the results were as follows:

(1)在水葱与香蒲组合种植中,二者皆长势良好。水葱在组合种植中,株高、鲜重与分株增长皆大于单一种植,其中鲜重增长在两种种植方式中存在显著差异,表明水葱在组合种植中生长更好。而香蒲在组合种植中与单一种值在各项指标上并无明显差异,这表明二者虽然生长型相同,但并不存在激烈的种间竞争,甚至还存在一定的促进作用。在去除氮磷效果方面,水葱单一种植去除总氮720.22mg,总磷138.20mg;香蒲单一种植去除总氮647.95mg,总磷133.57mg。水葱的营养去除能力略强。(1) In the combined planting of water onion and cattail, both grow well. The plant height, fresh weight and ramet growth of water onion in combination planting were all larger than those in single planting, and the fresh weight growth was significantly different between the two planting methods, indicating that water onion grows better in combination planting. However, there was no significant difference in the indicators of cattail in combination planting and single species value, which indicated that although the two had the same growth type, there was no fierce interspecific competition, and even had a certain promotion effect. In terms of removal of nitrogen and phosphorus, the single planting of water onion removes 720.22 mg of total nitrogen and 138.20 mg of total phosphorus; the single planting of cattail removes 647.95 mg of total nitrogen and 133.57 mg of total phosphorus. The nutrient removal ability of water onion is slightly stronger.

(2)在鸢尾与菖蒲组合种植中,菖蒲长势明显优于鸢尾。鸢尾在单一种值中,株高、鲜重与叶片增长皆大于组合种植,其中株高与鲜重在两种种植方式中存在显著差异,这表明在组合种植中,种间竞争作用较强,菖蒲处于优势地位,而鸢尾竞争能力较弱。在去除氮磷效果方面,鸢尾单一种植去除总氮684.62mg,总磷139.20mg;菖蒲单一种植去除总氮712.23mg,总磷160.99mg。菖蒲的营养去除能力略强。(2) In the combined planting of iris and iris, the growth of iris was significantly better than that of iris. In the single species value, the plant height, fresh weight and leaf growth of iris are all larger than those of the combination planting, and the plant height and fresh weight are significantly different in the two planting methods, which indicates that the interspecific competition is stronger in the combination planting. Calamus is in a dominant position, while iris is less competitive. In terms of removal of nitrogen and phosphorus, the single planting of iris removed 684.62 mg of total nitrogen and 139.20 mg of total phosphorus; the single planting of iris removed 712.23 mg of total nitrogen and 160.99 mg of total phosphorus. Calamus has slightly stronger nutrient removal ability.

(3)在美人蕉与再力花组合种植中,二者长势良好,美人蕉分株与叶片增长高于单一种植,但株高与鲜重增长小于单一种植。再力花的株高、鲜重与叶片增长皆小于单一种植。这表明相比于单一种植,两种植物在组合种植中的生长皆受到一定的抑制,使得二者在组合种植中的生长状况均弱于单一种植。在去除水体氮磷效果方面,美人蕉单一种植去除总氮791.20mg,总磷189.26mg;再力花单一种植去除总氮754.91mg,总磷144.66mg。美人蕉的营养去除能力略强。(3) In the combined planting of Canna and Zailihua, both grow well, the growth of canna ramets and leaves is higher than that of single planting, but the growth of plant height and fresh weight is smaller than that of single planting. The plant height, fresh weight and leaf growth of Zailihua were smaller than those of monoculture. This shows that the growth of the two plants in the combined planting is inhibited to a certain extent compared with the single planting, so that the growth conditions of the two plants in the combined planting are both weaker than those of the single planting. In terms of the removal of nitrogen and phosphorus in water, canna monoculture removed 791.20 mg of total nitrogen and 189.26 mg of total phosphorus; Zailihua monoculture removed 754.91 mg of total nitrogen and 144.66 mg of total phosphorus. The nutrient removal ability of canna is slightly stronger.

(4)在六种植物混种中,水葱的株高与分株增长低于单一种植,鲜重增长高于单一种植,表明水葱在单一种植与混合种植中长势相当。香蒲的株高、鲜重、株高增长与存活率皆显著低于单一种植,表明香蒲在六种植物混种中生长受到抑制。鸢尾的株高、鲜重与株高增长皆显著低于单一种植,菖蒲株高与鲜重增长高于单一种植,而叶片增长低于单一种植,表明菖蒲在混种中的生长情况要优于鸢尾。美人蕉与再力花的株高、鲜重与株高增长皆显著高于单一种植,这表明二者在混种中皆生长良好。(4) In the mixed planting of the six plants, the plant height and ramet growth of Scallion were lower than those of single planting, and the growth of fresh weight was higher than that of single planting, which indicated that the growth of Scallion was equal in single planting and mixed planting. The plant height, fresh weight, plant height growth and survival rate of cattail were significantly lower than those of monoculture, indicating that the growth of cattail was inhibited in the mixed planting of six species. The plant height, fresh weight and plant height growth of iris were significantly lower than those of single planting, while the growth of iris plant height and fresh weight were higher than those of single planting, while the growth of leaves was lower than that of single planting, indicating that the growth of iris in mixed species was better than that of single planting. iris. The plant height, fresh weight and plant height growth of Canna and Zailihua were significantly higher than those of single planting, which indicated that both grew well in mixed planting.

(5)六种植物混合种植对于水体中氮磷含量的去除效果最优,在两个月的培养中,其吸收去除总氮756.07mg,总磷为173.30mg,分别占添加总量的93%与91%,显著高于六种植物单一种植或组合种植的平均水平。在不断添加氮磷的情况下,水体中的氮磷从初始的总氮12mg/L、总磷4mg/L分别降到1.93mg/L与0.56mg/L,这表明六种挺水植物的生长型差异拉开了物种间的生态位,进一步造成不同种类植物在资源利用上的生态位互补,促进了群落整体对于氮磷的吸收。(5) The mixed planting of the six plants has the best effect on the removal of nitrogen and phosphorus in the water body. In the two-month cultivation, the total nitrogen absorbed and removed was 756.07 mg, and the total phosphorus was 173.30 mg, accounting for 93% of the total amount added. With 91%, significantly higher than the average of six plant monoculture or combination planting. Under the condition of continuous addition of nitrogen and phosphorus, the nitrogen and phosphorus in the water body decreased from the initial total nitrogen of 12 mg/L and total phosphorus of 4 mg/L to 1.93 mg/L and 0.56 mg/L, respectively, which indicated that the growth of the six emergent plants The differences in the types of plants open up the niches between species, which further leads to the complementation of the ecological niches of different species of plants in resource utilization, and promotes the absorption of nitrogen and phosphorus by the community as a whole.

(6)在单一种植中,六种植物去除水体的总氮总磷能力存在差异,去除氮的能力从大到小依次为:美人蕉、再力花、水葱、菖蒲、鸢尾与香蒲,去除磷的能力从大到小依次为:美人蕉、菖蒲、再力花、水葱、鸢尾与菖蒲。(6) In monoculture, there are differences in the ability of the six plants to remove total nitrogen and total phosphorus from water. The ability to remove nitrogen from large to small is: Canna, Zailihua, Scallion, calamus, iris and cattail. The abilities from largest to smallest are: canna, calamus, zailihua, water onion, iris and calamus.

以上所述仅为本发明的较佳实施例,并不用以限制本发明,凡在本发明的精神和原则之内,所作的任何修改、等同替换、改进等,均应包含在本发明的保护范围之内。The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection of the present invention. within the range.

Claims (6)

1.一种适用于城市景观水体修复的植物筛选方法,其特征在于,收集观赏性挺水植物水葱、香蒲、鸢尾、菖蒲、美人蕉、再力花的繁殖材料,分别按以下方式种植培育于装载有人工湿地基质的收纳盒内:1. a kind of plant screening method that is applicable to the restoration of urban landscape water body, it is characterized in that, collect the breeding material of ornamental emergent water plants scallion, cattail, iris, calamus, canna, reli flower, plant and cultivate in the following manner respectively. In the storage box loaded with the constructed wetland matrix: 1)每种植物单独种植于收纳盒内;1) Each plant is planted in the storage box separately; 2)将生长型相同的植物组合种植于收纳盒内;2) Plant the same plant combination in the growth type in the storage box; 包括以下步骤:Include the following steps: 1)定期添加营养;1) Regularly add nutrition; 2)监测植物生长情况以及水质指标;2) Monitor plant growth and water quality indicators; 3)根据植物的生长状况以及水体内氮磷元素的移除效果判断不同植物物种的竞争力强弱,从而进行筛选。3) Judging the competitiveness of different plant species according to the growth status of plants and the removal effect of nitrogen and phosphorus elements in the water, so as to screen. 2.根据权利要求1所述一种适用于城市景观水体修复的植物筛选方法,其特征在于,人工湿地基质主要由河沙构成,将河沙用自来水冲洗5-6遍,直至出水澄清,将清洗后的泥沙用0.2mol/L的稀盐酸浸泡1-2天,然后依次用自来水与蒸馏水冲洗,去除泥沙中的可溶性营养物质,以防止泥沙中原有的氮磷影响测量结果。2. a kind of plant screening method suitable for urban landscape water restoration according to claim 1, is characterized in that, the artificial wetland matrix is mainly made up of river sand, and the river sand is washed 5-6 times with tap water until the water is clarified, and the The cleaned sediment is soaked in 0.2mol/L dilute hydrochloric acid for 1-2 days, and then washed with tap water and distilled water in turn to remove the soluble nutrients in the sediment, so as to prevent the original nitrogen and phosphorus in the sediment from affecting the measurement results. 3.根据权利要求1所述一种适用于城市景观水体修复的植物筛选方法,其特征在于,收纳箱的尺寸规格为长63cm×宽45cm×高39cm,每个收纳箱均种植12株植物,故植物栽种的密度为43.5株/m2,植物的根茎固定在8cm深的泥沙中,培养期间水位维持在14cm-18cm之间。3. a kind of plant screening method suitable for urban landscape water body restoration according to claim 1, is characterized in that, the size specification of the storage box is length 63cm × width 45cm × height 39cm, and each storage box is all planted with 12 plants, Therefore, the planting density was 43.5 plants/m 2 , the rhizomes of the plants were fixed in the 8cm-deep sediment, and the water level was maintained between 14cm-18cm during the cultivation period. 4.根据权利要求1所述一种适用于城市景观水体修复的植物筛选方法,其特征在于,植物的生长环境为温室静水条件,培养水体的初始状态为10%的霍格兰营养液,其总氮浓度为12mg/L,总磷浓度为4mg/L,体积为30L;在植物培养的两个月期间,每5天进行营养液的添加,使用氯化铵、磷酸二氢钾作为营养液中氮磷的成分,每次添加的营养液中氮总量为70mg,磷总量为10mg,对于不同的植物组合,采取定量添加氮磷并用蒸馏水定容至统一水位的原则。4. a kind of plant screening method suitable for urban landscape water body restoration according to claim 1, is characterized in that, the growth environment of plant is greenhouse still water condition, and the initial state of cultivating water body is 10% Hoagland nutrient solution, its The total nitrogen concentration was 12mg/L, the total phosphorus concentration was 4mg/L, and the volume was 30L; during the two-month period of plant cultivation, the nutrient solution was added every 5 days, and ammonium chloride and potassium dihydrogen phosphate were used as the nutrient solution For the composition of nitrogen and phosphorus, the total amount of nitrogen in the nutrient solution added each time is 70mg, and the total amount of phosphorus is 10mg. For different plant combinations, the principle of quantitatively adding nitrogen and phosphorus and distilling the volume to a uniform water level is adopted. 5.根据权利要求1所述一种适用于城市景观水体修复的植物筛选方法,其特征在于,培养箱置于温室大棚内进行培养,8:00-20:00的时间段温度设置为30℃,20:00-8:00的时间段温度设置为25℃,湿度控制在50%;在8:00-20:00的时间段使用钠灯对植物进行光照补充,培养期间保持良好通风。5. a kind of plant screening method suitable for urban landscape water body restoration according to claim 1, is characterized in that, incubator is placed in greenhouse to cultivate, and the time period temperature of 8:00-20:00 is set to 30 ℃ , during the time period of 20:00-8:00, the temperature was set to 25 °C, and the humidity was controlled at 50%; the sodium lamp was used to supplement the light for the plants during the time period of 8:00-20:00, and good ventilation was maintained during the cultivation period. 6.一种适用于城市景观水体修复的植物群落构建方法,其特征在于,收集观赏性挺水植物水葱、香蒲、鸢尾、菖蒲、美人蕉、再力花的繁殖材料,并混合种植培育于装载有人工湿地基质的收纳盒内,构建群落,通过不同植物之间的生态位差异,以期弱化种间竞争、促进植物对于富营养化水体中氮磷的吸收与利用。6. A plant community construction method suitable for urban landscape water body restoration is characterized in that, collecting the propagation materials of ornamental emergent water plants scallion, cattail, iris, calamus, canna, and reliflower, and mixed planting and cultivating in the loading In a storage box with a constructed wetland matrix, a community is constructed, and through the niche differences between different plants, in order to weaken the competition between species and promote the absorption and utilization of nitrogen and phosphorus in eutrophic water by plants.
CN202210109124.8A 2022-01-28 2022-01-28 A method for plant screening and community construction suitable for urban landscape water restoration Pending CN114455710A (en)

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