CN114451305A - Rapid seedling raising equipment for tissue culture of color-leaf osmanthus fragrans and culture method thereof - Google Patents
Rapid seedling raising equipment for tissue culture of color-leaf osmanthus fragrans and culture method thereof Download PDFInfo
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- CN114451305A CN114451305A CN202210161581.1A CN202210161581A CN114451305A CN 114451305 A CN114451305 A CN 114451305A CN 202210161581 A CN202210161581 A CN 202210161581A CN 114451305 A CN114451305 A CN 114451305A
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- 235000019083 Osmanthus fragrans Nutrition 0.000 title claims abstract description 42
- 244000242564 Osmanthus fragrans Species 0.000 title claims abstract description 42
- 238000012136 culture method Methods 0.000 title abstract description 12
- 230000000694 effects Effects 0.000 claims abstract description 30
- 238000003825 pressing Methods 0.000 claims abstract description 17
- 230000008093 supporting effect Effects 0.000 claims abstract description 10
- 238000012216 screening Methods 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims description 46
- 241000196324 Embryophyta Species 0.000 claims description 42
- 238000007790 scraping Methods 0.000 claims description 26
- 238000001125 extrusion Methods 0.000 claims description 16
- 239000001963 growth medium Substances 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 230000006978 adaptation Effects 0.000 claims description 8
- 239000011521 glass Substances 0.000 claims description 8
- 238000000605 extraction Methods 0.000 claims description 7
- 239000011148 porous material Substances 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 235000019082 Osmanthus Nutrition 0.000 claims description 6
- 241000333181 Osmanthus Species 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 6
- 238000011010 flushing procedure Methods 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 6
- 230000007246 mechanism Effects 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 238000007873 sieving Methods 0.000 claims description 4
- 239000000758 substrate Substances 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
- 230000002378 acidificating effect Effects 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 230000001680 brushing effect Effects 0.000 claims description 3
- 238000005520 cutting process Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 230000001788 irregular Effects 0.000 claims description 3
- 229950008882 polysorbate Drugs 0.000 claims description 3
- 229920000136 polysorbate Polymers 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000008223 sterile water Substances 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 230000003139 buffering effect Effects 0.000 claims 1
- 230000009471 action Effects 0.000 description 3
- 230000003044 adaptive effect Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
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- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a rapid seedling culture device for tissue culture of color-leaf osmanthus fragrans and a culture method thereof. According to the color-leaf osmanthus fragrans tissue culture rapid seedling raising equipment and the culture method thereof, the upper top pressing shell arranged at the bottom of the top cover shell limits the supporting bars, so that the overall pressure bearing effect on the annular frame bar is improved, and meanwhile, when an internal screening device takes a sample, a certain elastic supporting effect can be achieved.
Description
Technical Field
The invention relates to the technical field of plant tissue culture, in particular to a rapid seedling raising device for tissue culture of color-leaf osmanthus fragrans and a culture method thereof.
Background
The tissue culture of plants is a new asexual propagation technology developed in recent decades based on the theory that plant cells have totipotency. Tissue culture of plants, also called in vitro culture in a broad sense, refers to a technique of separating desired tissues, organs or cells, protoplasts, etc. from plant bodies, inoculating them on a culture medium containing various nutrients and plant hormones under aseptic conditions by aseptic manipulation to obtain regenerated whole plants or to produce other products having economic value.
When the existing tissue culture seedling raising equipment and culture method for color-leaf osmanthus fragrans are used for culturing the color-leaf osmanthus fragrans tissues, the plant tissues cannot be treated in an aseptic state, so that the culture effect is poor during later-stage tissue culture of the color-leaf osmanthus fragrans.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a rapid seedling raising device for tissue culture of color-leaf osmanthus fragrans and a culture method thereof, and solves the problem that when the color-leaf osmanthus fragrans tissue is cultured, the plant tissue cannot be treated under the sterile state, so that the culture effect is poor in the later-period tissue culture of the color-leaf osmanthus fragrans.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: the utility model provides a various leaf sweet-scented osmanthus tissue culture equipment of growing seedlings fast, includes the glass shade, the bottom fixedly connected with top hood shell of glass shade, the side surface fixedly connected with of hood shell draws the window, the bottom fixedly connected with of top hood shell supports the base, fixedly connected with slow compression ring is located to the centre at top hood shell top, extraction element in the inside fixedly connected with of top hood shell.
Preferably, the inner extraction device comprises an upper top pressing shell, the bottom of the upper top pressing shell is fixedly connected to the bottom of the inner cavity of the top cover shell, and a supporting strip is fixedly connected to the upper surface of the upper top pressing shell.
Preferably, the top ends of the supporting bars are fixedly connected with ring frame rods, the inner side faces of the ring frame rods are fixedly connected with screening devices, the slotted holes in the bottom of the top cover shell are fixedly connected with plastic cushion blocks, and culture substrates are sleeved between opposite faces of the plastic cushion blocks. Through setting up the last top pressure shell in top hood shell bottom, through restricting its support bar, improve the whole pressure-bearing effect to the ring hack lever, during the sieving mechanism sample in inside simultaneously, can reach certain elastic support effect.
Preferably, the screening device comprises a side frame rod, a limiting retaining ring is arranged inside the side frame rod, an autorotation shaft is rotatably connected inside the side frame rod, and a pressing and folding rod is fixedly connected to the outer surface of the autorotation shaft.
Preferably, the bottom of the side frame rod is fixedly connected with an extrusion pore plate, the bottom of the lower pressing folding rod is in extrusion fit with the top of the extrusion pore plate, and the bottom of the limiting retaining ring is fixedly connected with a scraping device. Through the spacing of spacing buckle to the rotation axis of rotation, can make its push down the folding rod and compress the extrusion orifice plate of bottom, can hug closely the surface that the sweet-scented osmanthus organized when guaranteeing the bottom scraping device operation, reach the effect of effective sample.
Preferably, the scraping device comprises a scraping frame rod, the top end of the scraping frame rod is fixedly connected to the bottom of the limiting retaining ring, and an inner concave hole is formed in the scraping frame rod in a sleeved mode.
Preferably, the inner chamber department cover of interior notch opening is equipped with erects the arc board, erect the bottom fixedly connected with end cone unit of arc board, establish the bullet piece in the top fixedly connected with of end cone unit, establish the surface of bullet piece and erect medial surface department looks extrusion adaptation of arc board in.
Preferably, the bottom cone unit comprises a conical ring plate, two sides of the conical ring plate are fixedly connected to the bottom of the erection arc plate, and two sides of the bottom of the conical ring plate are fixedly connected with a sleeve frame.
Preferably, the side cover of cover frame both sides is equipped with the adaptation slider, the top fixedly connected with load spring of adaptation slider, load spring's top fixed connection is at the top of cover frame, the bottom fixedly connected with arcuation scraper of adaptation slider. When the arc-shaped scraper is attached to the surface of the color-leaf osmanthus fragrans tissue, the bearing spring can be used for achieving pressure attachment to the surface, and the surface can be scraped through micro-pressure, so that the scraping effect of the structural tissue is guaranteed.
A method for tissue culture and rapid seedling culture of color-leaf osmanthus fragrans comprises the following steps:
selecting materials according to the cultivation purpose, selecting sterile materials in robust plant tissues, and placing the sterile materials in a sterile empty bottle after the materials are adopted; preparing a culture medium, adding agar into the prepared culture medium, heating and dissolving, adjusting the pH to be slightly acidic, and subpackaging in a sterile culture dish of 200ml with 50ml per bottle when the culture medium is hot; after the culture medium is cooled and solidified, placing the culture dish in an autoclave for sterilization at 120 ℃ for 20 min; through the highly sterile treatment of the material, the plant tissue can be effectively cultured in a sterile way, so that the high-efficiency culture effect is achieved;
removing the unused part of the collected plant material, cleaning the required part by using water added with a certain amount of polysorbate, removing stains by adhering to the surface of the plant material, brushing by using a high-density soft hair brush, cleaning residual impurities on the surface, cutting the material into particles with proper sizes, wiping the residual moisture on the surface, and storing the particles by using a gauze tape;
step three, infiltrating and sterilizing the surface of the material; placing the materials in the gauze on the surface of a super clean bench, unfastening the gauze, infiltrating the internal plant materials with 70% alcohol for 15-20 seconds, immediately flushing with clear water after the materials are infiltrated, flushing the redundant alcohol with sterile water, drying the materials, and placing the materials into a culture dish;
step four, placing the culture dish filled with the materials into the color-leaf osmanthus fragrans tissue culture rapid seedling raising equipment in an open manner, wherein the color-leaf osmanthus fragrans tissue culture rapid seedling raising equipment can be used for micro-slicing of the osmanthus fragrans tissues on the surfaces of the culture dishes in different stages, slicing, processing and observing, and meanwhile, organizing plants into long plants to achieve an effective guiding effect; the slices in different periods can effectively observe plant tissues in real time, and improve the culture effect of the tissues.
Cultivating the cultivated plants to the land from the culture dish, and carrying out irregular stage type simulated weather watering on the cultivated plants in a stage watering mode to enable the plants to adapt to an external environment; and simultaneously recording the residual nutrient substances in the culture dish to provide substances required by the culture medium for the next preparation.
(III) advantageous effects
The invention provides a rapid seedling raising device for tissue culture of color-leaf osmanthus fragrans and a culture method thereof. The method has the following beneficial effects:
the color-leaf osmanthus fragrans tissue culture rapid seedling raising equipment and the culture method thereof have the advantages that the supporting bars are limited by the upper jacking shell arranged at the bottom of the top cover shell, so that the overall pressure bearing effect on the annular frame bar is improved, and meanwhile, certain elastic supporting effect can be achieved when the internal screening device takes a sample.
According to the rapid seedling raising equipment for tissue culture of the color-leaf osmanthus fragrans and the culture method thereof, the rotation rotary shaft is limited through the limiting retaining ring, so that the pressing folding rod can be pressed down to compress the extrusion pore plate at the bottom, the bottom scraping device can be ensured to be tightly attached to the surface of the osmanthus fragrans tissue during operation, and the effect of effective sampling is achieved.
When the arc-shaped scraper is attached to the surface of the color-leaf osmanthus fragrans tissue, the bearing spring can be used for achieving pressure attachment to the surface, and the surface can be scraped off through micro-pressure, so that the scraping effect of the structural tissue is guaranteed.
According to the rapid seedling raising equipment for tissue culture of the color-leaf osmanthus fragrans and the culture method thereof, through high-degree aseptic treatment on materials, the plant tissues can be effectively subjected to aseptic culture, and the efficient culture effect is achieved; the slices in different periods can effectively observe plant tissues in real time, and improve the culture effect of the tissues.
Drawings
FIG. 1 is a flow chart of the tissue culture and rapid seedling culture method of Osmanthus fragrans with color leaves of the present invention;
FIG. 2 is a schematic structural view of the tissue culture rapid seedling raising equipment for color-leaf osmanthus fragrans of the present invention;
FIG. 3 is a schematic view of the internal extraction apparatus of the present invention;
FIG. 4 is a schematic structural view of a screening apparatus according to the present invention;
FIG. 5 is a schematic view of the scraping apparatus of the present invention;
FIG. 6 is a schematic structural diagram of a bottom cone unit according to the present invention.
In the figure: 1. a glass shade; 2. a top sleeve shell; 3. a support base; 4. a buffer ring; 5. extracting a window; 6. an internal extraction device; 61. culturing a substrate; 62. a screening device; 621. a side frame bar; 622. a self-rotating shaft; 623. pressing down the folding rod; 624. a scraping device; 6241. erecting an arc plate; 6242. a scraping frame rod; 6244. an inner concave hole; 6245. a spring block is arranged in the spring block; a. a bottom cone unit; a1, conical ring plate; a2, a sleeve frame; a3, arc-shaped scraper; a4, adapting the slide block; a5, a force bearing spring; 625. extruding the orifice plate; 626. a limit retaining ring; 63. plastic cushion blocks; 64. pressing the shell upwards; 65. a supporting strip; 66. a ring frame rod.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example one
As shown in fig. 2-4, the present invention provides a technical solution: the utility model provides a various leaf sweet-scented osmanthus tissue culture equipment of growing seedlings fast, includes glass shade 1, the bottom fixedly connected with top hood shell 2 of glass shade 1, the side surface fixedly connected with of hood shell 2 draws window 5, the bottom fixedly connected with of top hood shell 2 supports base 3, fixedly connected with slow clamping ring 4 of the centre department at 2 tops of top hood shell, extraction element 6 in the inside fixedly connected with of top hood shell 2.
Preferably, the inner extraction device 6 comprises an upper top pressing shell 64, the bottom of the upper top pressing shell 64 is fixedly connected to the bottom of the inner cavity of the top cover shell 2, and a supporting bar 65 is fixedly connected to the upper surface of the upper top pressing shell 64.
Preferably, the top end of the supporting bar 65 is fixedly connected with a ring frame bar 66, the inner side surface of the ring frame bar 66 is fixedly connected with a screening device 62, a plastic cushion block 63 is fixedly connected to the slotted hole at the bottom of the top cover shell 2, and a culture substrate 61 is sleeved between the opposite surfaces of the plastic cushion block 63. Through setting up the last top pressure shell 64 in top cover shell 2 bottom, through restricting its support bar 65, improve the whole pressure-bearing effect to ring frame pole 66, when the sieving mechanism 62 sample in inside simultaneously, can reach certain elastic support effect.
Preferably, the screening device 62 includes a side frame bar 621, a limit buckle 626 is disposed inside the side frame bar 621, a rotation shaft 622 is rotatably connected inside the side frame bar 621, and a pressing and folding bar 623 is fixedly connected to an outer surface of the rotation shaft 622.
Preferably, the bottom of the side frame bar 621 is fixedly connected with an extrusion hole plate 625, the bottom of the lower folding bar 623 is in extrusion fit with the top of the extrusion hole plate 625, and the bottom of the limiting retaining ring 626 is fixedly connected with a scraping device 624. Through the limit of the limit retaining ring 626 on the self-rotation shaft 622, the pressing and folding rod 623 can be pressed down to compress the extrusion pore plate 625 at the bottom, the surface of the osmanthus fragrans tissues can be tightly attached to the bottom scraping device 624 during operation, and the effect of effective sampling is achieved.
The first embodiment has the following working steps:
step one, set up the top pressure shell 64 in the bottom of hood shell 2, through restricting its support bar 65, improve the whole pressure-bearing effect to ring frame pole 66, when the sieving mechanism 62 sample in inside simultaneously, can reach certain elastic support effect.
And step two, the limiting retaining ring 626 limits the rotation shaft 622, so that the pressing and folding rod 623 can be pressed down to compress the extrusion pore plate 625 at the bottom, the bottom scraping device 624 can be ensured to be tightly attached to the surface of the osmanthus fragrans tissue during operation, and the effect of effective sampling is achieved.
Example two
As shown in fig. 5 to 6, on the basis of the first embodiment, the present invention provides a technical solution: the scraping device 624 comprises a scraping frame bar 6242, the top end of the scraping frame bar 6242 is fixedly connected to the bottom of the limiting retaining ring 626, and an inner concave hole 6244 is sleeved inside the scraping frame bar 6242.
Preferably, an inner cavity of the inner concave hole 6244 is sleeved with an erection arc plate 6241, the bottom of the erection arc plate 6241 is fixedly connected with a bottom cone unit a, the top of the bottom cone unit a is fixedly connected with an inner elastic block 6245, and the outer surface of the inner elastic block 6245 is in extrusion fit with the inner side surface of the erection arc plate 6241.
Preferably, the bottom cone unit a comprises a conical ring plate a1, both sides of the conical ring plate a1 are fixedly connected to the bottom of the erection arc plate 6241, and sleeve frames a2 are fixedly connected to both sides of the bottom of the conical ring plate a 1.
Preferably, the side edges of the two sides of the sleeve frame a2 are sleeved with an adaptive slider a4, the top of the adaptive slider a4 is fixedly connected with a force bearing spring a5, the top of the force bearing spring a5 is fixedly connected with the top of the sleeve frame a2, and the bottom of the adaptive slider a4 is fixedly connected with an arc-shaped scraper a 3. When the arc-shaped scraper a3 is attached to the surface of the color-leaf osmanthus fragrans tissue, the bearing spring a5 can be used for achieving pressure attachment to the surface, and the surface can be scraped through micro-pressure, so that the scraping effect of the structure tissue is ensured.
The second embodiment has the following working steps:
when the arc-shaped scraper a3 is attached to the surface of the color-leaf osmanthus fragrans tissue, the bearing spring a5 can be used for achieving pressure attachment to the surface, and the surface can be scraped through micro-pressure, so that the scraping effect of the structure tissue is ensured.
EXAMPLE III
As shown in fig. 1, on the basis of the first embodiment and the second embodiment, the present invention provides a technical solution: a method for tissue culture and rapid seedling culture of color-leaf osmanthus fragrans comprises the following steps:
selecting materials according to the cultivation purpose, selecting sterile materials in robust plant tissues, and placing the sterile materials in a sterile empty bottle after the materials are adopted; preparing a culture medium, adding agar into the prepared culture medium, heating and dissolving, adjusting the pH to be slightly acidic, and subpackaging in a sterile culture dish of 200ml with 50ml per bottle when the culture medium is hot; after the culture medium is cooled and solidified, placing the culture dish in an autoclave for sterilization at 120 ℃ for 20 min; through the highly sterile treatment of the material, the plant tissue can be effectively cultured in a sterile way, so that the high-efficiency culture effect is achieved;
removing the unused part of the collected plant material, cleaning the required part by using water added with a certain amount of polysorbate, removing stains by adhering to the surface of the plant material, brushing by using a high-density soft hair brush, cleaning residual impurities on the surface, cutting the material into particles with proper sizes, wiping the residual moisture on the surface, and storing the particles by using a gauze tape;
step three, infiltrating and sterilizing the surface of the material; placing the materials in the gauze on the surface of a super clean bench, unfastening the gauze, infiltrating the internal plant materials with 70% alcohol for 15-20 seconds, immediately flushing with clear water after the materials are infiltrated, flushing the redundant alcohol with sterile water, drying the materials, and placing the materials into a culture dish;
step four, placing the culture dish filled with the materials into the color-leaf osmanthus fragrans tissue culture rapid seedling raising equipment in an open manner, wherein the color-leaf osmanthus fragrans tissue culture rapid seedling raising equipment can be used for micro-slicing of the osmanthus fragrans tissues on the surfaces of the culture dishes in different stages, slicing, processing and observing, and meanwhile, organizing plants into long plants to achieve an effective guiding effect; the slices in different periods can effectively observe plant tissues in real time, and improve the culture effect of the tissues.
Cultivating the cultivated plants to the land from the culture dish, and carrying out irregular stage type simulated weather watering on the cultivated plants in a stage watering mode to enable the plants to adapt to an external environment; and simultaneously recording the residual nutrient substances in the culture dish to provide substances required by the culture medium for the next preparation.
The third embodiment has the following working steps:
step one, through the highly sterile treatment of the material, the plant tissue can be effectively cultured in a sterile way, so that the efficient culture effect is achieved;
and step two, slicing in different periods can effectively observe plant tissues in real time, so that the tissue culture effect is improved.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. The term "comprising", without further limitation, means that the element so defined is not excluded from the group consisting of additional identical elements in the process, method, article, or apparatus that comprises the element.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. The utility model provides a quick equipment of growing seedlings of various leaf sweet-scented osmanthus tissue culture, includes glass shade (1), its characterized in that: the glass shade is characterized in that a top cover shell (2) is fixedly connected to the bottom of the glass shade (1), a window (5) is extracted from the side surface of the cover shell (2), a supporting base (3) is fixedly connected to the bottom of the top cover shell (2), a buffering ring (4) is fixedly connected to the middle of the top cover shell (2) top, and an extracting device (6) is arranged in the inner fixedly connected with of the top cover shell (2).
2. The rapid seedling raising equipment for tissue culture of color-leaf osmanthus according to claim 1, characterized in that: interior extraction element (6) are including last top pressure shell (64), the bottom fixed connection of going up top pressure shell (64) is in the bottom of hood shell (2) inner chamber, the last fixed surface who goes up top pressure shell (64) is connected with support bar (65).
3. The rapid seedling raising equipment for tissue culture of color-leaf osmanthus fragrans according to claim 2, characterized in that: the top fixed connection of support bar (65) has ring hack lever (66), the medial surface fixedly connected with sieving mechanism (62) of ring hack lever (66), slotted hole fixedly connected with plastic cushion block (63) of overhead guard shell (2) bottom, the cover is equipped with culture substrate (61) between the opposite face of plastic cushion block (63).
4. The rapid seedling raising equipment for tissue culture of color-leaf osmanthus fragrans according to claim 3, characterized in that: the screening device (62) comprises a side frame rod (621), a limiting snap ring (626) is arranged inside the side frame rod (621), a self-rotation shaft (622) is rotatably connected inside the side frame rod (621), and a pressing and folding rod (623) is fixedly connected to the outer surface of the self-rotation shaft (622).
5. The rapid seedling raising equipment for tissue culture of color-leaf osmanthus fragrans according to claim 4, characterized in that: the bottom of the side frame rod (621) is fixedly connected with an extrusion pore plate (625), the bottom of the lower pressing folding rod (623) is in extrusion fit with the top of the extrusion pore plate (625), and the bottom of the limiting retaining ring (626) is fixedly connected with a scraping device (624).
6. The rapid seedling raising equipment for tissue culture of color-leaf osmanthus fragrans according to claim 5, characterized in that: the scraping device (624) comprises a scraping frame rod (6242), the top end of the scraping frame rod (6242) is fixedly connected to the bottom of the limiting retaining ring (626), and an inner concave hole (6244) is sleeved in the scraping frame rod (6242).
7. The rapid seedling raising equipment for tissue culture of color-leaf osmanthus fragrans according to claim 6, characterized in that: the inner cavity department cover of interior notch (6244) is equipped with erects arc board (6241), erect the bottom fixedly connected with end cone unit (a) of arc board (6241), establish bullet piece (6245) in the top fixedly connected with of end cone unit (a), establish the surface of bullet piece (6245) and erect the medial surface department looks extrusion adaptation of arc board (6241) in.
8. The rapid seedling raising equipment for tissue culture of color-leaf osmanthus according to claim 7, characterized in that: the bottom cone unit (a) comprises a conical ring plate (a1), two sides of the conical ring plate (a1) are fixedly connected to the bottom of the erection arc plate (6241), and two sides of the bottom of the conical ring plate (a1) are fixedly connected with sleeve frames (a 2).
9. The rapid seedling raising equipment for tissue culture of color-leaf osmanthus fragrans according to claim 8, characterized in that: the side cover of cover frame (a2) both sides is equipped with adaptation slider (a4), the top fixedly connected with load spring (a5) of adaptation slider (a4), the top fixed connection of load spring (a5) is at the top of cover frame (a2), the bottom fixedly connected with arcuation scraper (a3) of adaptation slider (a 4).
10. A method for tissue culture and rapid seedling culture of color-leaf osmanthus fragrans is characterized by comprising the following steps: the method comprises the following steps:
selecting materials according to the cultivation purpose, selecting sterile materials in robust plant tissues, and placing the sterile materials in a sterile empty bottle after the materials are adopted; preparing a culture medium, adding agar into the prepared culture medium, heating and dissolving, adjusting the pH to be slightly acidic, and subpackaging in 200ml sterile culture dishes with 50ml per bottle when the culture medium is hot; after the culture medium is cooled and solidified, placing the culture dish in an autoclave for sterilization at 120 ℃ for 20 min;
removing the unused part of the collected plant material, cleaning the required part by using water added with a certain amount of polysorbate, removing stains by adhering to the surface of the plant material, brushing by using a high-density soft hair brush, cleaning residual impurities on the surface, cutting the material into particles with proper sizes, wiping the residual moisture on the surface, and storing the particles by using a gauze tape;
step three, infiltrating and sterilizing the surface of the material; placing the materials in the gauze on the surface of a super clean bench, unfastening the gauze, infiltrating the internal plant materials with 70% alcohol for 15-20 seconds, immediately flushing with clear water after the materials are infiltrated, flushing the redundant alcohol with sterile water, drying the materials, and placing the materials into a culture dish;
step four, placing the culture dish filled with the materials into the color-leaf osmanthus fragrans tissue culture rapid seedling raising equipment in an open manner, wherein the color-leaf osmanthus fragrans tissue culture rapid seedling raising equipment can be used for micro-slicing of the osmanthus fragrans tissues on the surfaces of the culture dishes in different stages, slicing, processing and observing, and meanwhile, organizing plants into long plants to achieve an effective guiding effect;
cultivating the cultivated plants to the land from the culture dish, and carrying out irregular stage type simulated weather watering on the cultivated plants in a stage watering mode to enable the plants to adapt to an external environment; and simultaneously recording the residual nutrient substances in the culture dish to provide substances required by the culture medium for the next preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210161581.1A CN114451305A (en) | 2022-02-22 | 2022-02-22 | Rapid seedling raising equipment for tissue culture of color-leaf osmanthus fragrans and culture method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210161581.1A CN114451305A (en) | 2022-02-22 | 2022-02-22 | Rapid seedling raising equipment for tissue culture of color-leaf osmanthus fragrans and culture method thereof |
Publications (1)
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|---|---|
| CN114451305A true CN114451305A (en) | 2022-05-10 |
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| CN202210161581.1A Pending CN114451305A (en) | 2022-02-22 | 2022-02-22 | Rapid seedling raising equipment for tissue culture of color-leaf osmanthus fragrans and culture method thereof |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4475728A (en) * | 1982-07-01 | 1984-10-09 | Haddad Arthur K | Toggle actuated bench clamp |
| WO2009089661A1 (en) * | 2008-01-15 | 2009-07-23 | Wong Lau | Clamp |
| CN103798147A (en) * | 2014-03-06 | 2014-05-21 | 梅州市状元红生态发展有限公司 | Cultivation method for osmanthus fragrans tissues |
| CN107020241A (en) * | 2017-03-29 | 2017-08-08 | 周庆川 | Knocking type mechanical picker |
| CN212487686U (en) * | 2020-05-09 | 2021-02-09 | 漳州天华食用菌有限公司 | Pleurotus eryngii planting box with light-shading function |
-
2022
- 2022-02-22 CN CN202210161581.1A patent/CN114451305A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4475728A (en) * | 1982-07-01 | 1984-10-09 | Haddad Arthur K | Toggle actuated bench clamp |
| WO2009089661A1 (en) * | 2008-01-15 | 2009-07-23 | Wong Lau | Clamp |
| CN103798147A (en) * | 2014-03-06 | 2014-05-21 | 梅州市状元红生态发展有限公司 | Cultivation method for osmanthus fragrans tissues |
| CN107020241A (en) * | 2017-03-29 | 2017-08-08 | 周庆川 | Knocking type mechanical picker |
| CN212487686U (en) * | 2020-05-09 | 2021-02-09 | 漳州天华食用菌有限公司 | Pleurotus eryngii planting box with light-shading function |
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