CN114441274A - Cell staining system, staining control method and related equipment - Google Patents
Cell staining system, staining control method and related equipment Download PDFInfo
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- CN114441274A CN114441274A CN202210102221.4A CN202210102221A CN114441274A CN 114441274 A CN114441274 A CN 114441274A CN 202210102221 A CN202210102221 A CN 202210102221A CN 114441274 A CN114441274 A CN 114441274A
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- 238000012360 testing method Methods 0.000 claims abstract description 5
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- 239000002699 waste material Substances 0.000 claims description 35
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- 239000006166 lysate Substances 0.000 description 11
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- 230000009286 beneficial effect Effects 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
Abstract
The invention relates to the technical field of cell staining, in particular to a cell staining system, a staining control method and related equipment. Wherein the cell staining system comprises: a staining component, wherein the staining component is provided with reagents required by cell staining; the test tube assembly is connected with the multi-way valve; a common fluid port connected to the multi-way valve, the common fluid port being for retaining the reagent in the cuvette assembly; and the cell staining chip is connected with the multi-way valve. The device eliminates the interference of bubbles on cell staining, so that the cell staining chip can accurately stain the target cells, the number of the target cells is determined to be accurate according to a staining result, the current state of illness of a patient can be accurately judged, and accurate reference is provided for targeted treatment.
Description
Technical Field
The invention relates to the technical field of cell staining, in particular to a cell staining system, a staining control method and related equipment.
Background
Currently, the majority of tumors are diagnosed clinically by means of imaging examinations, traditional tumor markers and tissue biopsies, which require an invasive tissue biopsy from a patient's body, which can be painful and risk the spread of cancer cells, and which are not suitable for repeated extraction.
Although the circulating tumor cell detection technology based on the microfluidic chip can separate and obtain the circulating tumor cells so as to facilitate subsequent research and analysis, the circulating tumor cells are greatly interfered during dyeing, the accuracy of determining the number of the circulating tumor cells is influenced, and the detection result is inaccurate.
Therefore, there is a need for a cell staining system, a method for controlling staining and a related apparatus, which at least partially solve the problems in the prior art.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art or the related art.
To this end, a first aspect of the invention provides a cell staining system.
In a second aspect, the invention provides a method of controlling a cell staining system.
A third aspect of the invention provides a computer-readable storage medium.
In view of this, according to a first aspect of the embodiments of the present application, there is provided a cell staining system, including:
a staining component, wherein the staining component is provided with reagents required by cell staining;
the test tube assembly is connected with the multi-way valve;
a common fluid port connected to the multi-way valve, the common fluid port being for retaining the reagent in the cuvette assembly;
and the cell staining chip is connected with the multi-way valve.
In one possible embodiment, the cell staining system further comprises:
and the waste liquid barrel is connected with the cell staining chip.
In one possible embodiment, the dyeing assembly comprises:
the placing frame is provided with a plurality of fixing holes;
the first placing part penetrates through the fixing hole, and lysis solution is placed on the first placing part;
the second placing part penetrates through the fixing hole, and fixing liquid is placed in the second placing part;
the third placing part penetrates through the fixing hole, and the dyeing solution is placed on the third placing part; labels attached to the first, second, and third placement portions;
the first placing portion, the second placing portion and the third placing portion are connected to the multi-way valve respectively.
In one possible embodiment, the cell staining system further comprises:
and the buffer solution conveying device is connected to the public liquid path port and is used for conveying the buffer solution to the public liquid path port.
In one possible embodiment, the buffer delivery device comprises:
a container connected to the common fluid path port for containing the buffer fluid;
a direction change valve provided between the accommodating portion and the common liquid path port;
and the pump body is connected with the reversing valve.
In one possible embodiment, the common fluid path port is provided with a helical section.
In one possible embodiment, the cell staining system further comprises:
and the controller is connected with the pump body and used for controlling the speed of the pump body for extracting the reagent from the staining component or the buffer solution from the accommodating part and controlling the flow rate of the reagent conveyed to the cell staining chip.
According to a second aspect of the embodiments of the present application, there is provided a control method of a cell staining system, for controlling the cell staining system according to any one of the above technical solutions, the control method comprising:
receiving a control instruction;
extracting the reagent of the staining component and the buffer of the buffer conveying device to convey into the cell staining chip according to the work content indicated by the control instruction.
In a possible embodiment, the steps of extracting the reagent of the staining component and extracting the buffer of the buffer delivery device to be delivered into the cell staining chip according to the work content indicated by the control instruction comprise:
in the case that the work content comprises washing, pumping the buffer solution to be filled into the cell staining chip, and discharging the buffer solution overflowing the cell staining chip into the waste liquid barrel;
under the condition that the working content comprises cracking, controlling the pump body to extract a cracking solution to fill the cell staining chip, and discharging the cracking solution overflowing the cell staining chip into the waste liquid barrel;
under the condition that the work content comprises fixation, controlling the pump body to extract fixing liquid to fill the cell staining chip, and discharging the fixing liquid overflowing the cell staining chip into the waste liquid barrel;
and under the condition that the work content comprises dyeing, controlling the pump body to pump the dyeing liquid to fill the cell dyeing chip, and discharging the dyeing liquid overflowing the cell dyeing chip into the waste liquid barrel.
According to a third aspect of the embodiments of the present application, there is provided a computer-readable storage medium, on which a computer program is stored, the computer program, when being executed by a processor, implementing the method for controlling a cell staining system according to any one of the above-mentioned aspects.
Compared with the prior art, the invention at least comprises the following beneficial effects: the cell staining system that this application embodiment provided, be provided with the staining subassembly, the multichannel valve, public liquid way port and cell staining chip, wherein, the staining subassembly has placed the required reagent of cell staining, through multichannel valve intercommunication staining subassembly, public liquid way port and cell staining chip, when needs are to the cell staining, the reagent in the staining subassembly is carried and is carried the buffer memory in to public liquid way port from the multichannel valve, with this interference of bubble between getting rid of test tube subassembly and the multichannel valve to the staining result, carry the reagent that prestores in public liquid way port to cell staining chip in, dye target cell through the cell staining chip. The device eliminates the interference of bubbles on cell staining, so that the cell staining chip can accurately stain the target cells, the number of the target cells is determined to be accurate according to a staining result, the current state of illness of a patient can be accurately judged, and accurate reference is provided for targeted treatment.
Additional advantages, objects, and features of the cell staining system, method of controlling staining, and related apparatus of the present invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Drawings
Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the specification. Also, like reference numerals are used to refer to like parts throughout the drawings. In the drawings:
FIG. 1 is a schematic diagram of a cell staining system according to the present application;
FIG. 2 is a schematic flow chart of a method of controlling the cell staining system of FIG. 1;
fig. 3 is a schematic structural diagram of a computer-readable storage medium according to an embodiment of the present application.
Wherein, the corresponding relationship between the reference numbers and the component names in fig. 1 is:
100 cell staining system, 110 staining subassembly, 111 first portion of placing, 112 second portion of placing, 113 third portion of placing, 120 multichannel valve, 130 public liquid way port, 131 spiral section, 140 cell staining chip, 150 waste liquid buckets, 160 buffer fluid conveyor, 161 portion of holding, 162 switching-over valve, 163 pump body.
Detailed Description
In order to better understand the technical solutions described above, the technical solutions of the embodiments of the present application are described in detail below with reference to the drawings and the specific embodiments, and it should be understood that the specific features of the embodiments and the embodiments of the present application are detailed descriptions of the technical solutions of the embodiments of the present application, and are not limitations of the technical solutions of the present application, and the technical features of the embodiments and the embodiments of the present application may be combined with each other without conflict.
In some examples, as shown in fig. 1, according to a first aspect of embodiments of the present application, there is provided a cell staining system 100 comprising: a staining module 110, wherein the staining module 110 is provided with reagents required for cell staining; a multi-way valve 120, the test tube assembly being connected to the multi-way valve 120; a common liquid path port 130 connected to the multi-path valve 120, the common liquid path port 130 being for retaining the reagent in the cuvette assembly; the cell staining chip 140 is connected to the multi-way valve 120.
Specifically, the cell staining system 100 is provided with a staining assembly 110, a multi-channel valve 120, a common liquid channel port 130 and a cell staining chip 140, wherein the staining assembly 110 is provided with reagents required for cell staining, and it can be understood that the staining assembly 110, the common liquid channel port 130 and the cell staining chip 140 are respectively communicated with the multi-channel valve 120 through a conduit.
Since a space is left in the catheter and the staining assembly 110 after the reagent is placed in the staining assembly 110, bubbles are generated, after the target cells are stained, the target cells are stored in the channels of the cell-staining chip 140, when the target cells are stained, the access of the multi-way valve 120 is adjusted to open the access between the staining assembly 110 and the common liquid path port 130, the reagent in the staining assembly 110 is conveyed from the multi-way valve 120 to the common liquid path port 130 for buffering, thereby removing air bubbles between the dyeing component 110 and the multi-way valve 120, adjusting the way of the multi-way valve 120 after the air bubbles are eliminated, to open a path between the common fluid path port 130 and the cell staining chip 140, and transfer a reagent pre-stored in the common fluid path port 130 into the cell staining chip 140 to stain target cells in the cell staining chip 140. The arrangement eliminates the interference of bubbles on cell dyeing operation, so that the cell dyeing chip 140 can accurately dye the target cells, the number of the target cells is determined to be accurate according to the dyeing result, the current state of illness of the patient can be accurately judged, and accurate reference is provided for targeted treatment.
Illustratively, the cell staining chip 140 may be a microfluidic chip, the microfluidic chip is provided with a plurality of channels, the microfluidic chip is provided with a converging-diverging unit, target cells in a reagent sample in the tube assembly can be converged by the converging-diverging unit, and a certain proportion of reagent fluid without the target cells can be separated to capture the target cells, and the target chip is stored in the channels and stained with the reagent in the staining assembly 110.
Illustratively, the target cell may be a circulating tumor cell.
In some examples, as shown in fig. 1, the cell staining system 100 further includes: the waste liquid tank 150 is connected to the cell staining chip 140.
It can be understood that the cell staining system 100 is further provided with a waste liquid barrel 150, the waste liquid barrel 150 is connected to the cell staining chip 140 through a conduit, specifically, before the cell staining operation is performed, the reagent in the staining assembly 110 needs to be conveyed into the cell staining chip 140 until the reagent overflows from the chip and flows into the waste liquid barrel 150 along the conduit, so as to discharge the air bubbles in the cell staining chip 140, further eliminating the interference of the air bubbles on the cell staining operation, so that the cell staining chip 140 stains the target cells accurately, and thus the number of the target cells is determined accurately according to the staining result, so that the current condition of the patient can be accurately determined, and an accurate reference is provided for the targeted therapy. And during the cell staining operation, the waste liquid in the staining process can be discharged out of the cell staining chip 140, so that the interference of the waste liquid on the detection result is avoided.
In some examples, as shown in fig. 1, the dyeing component 110 includes: the rack is provided with a plurality of fixing holes; a first placing section 111 penetrating the fixing hole, the first placing section 111 placing a lysis solution; a second placing part 112 penetrating the fixing hole, the second placing part 112 placing a fixing liquid; a third placing part 113 inserted into the fixing hole, wherein the third placing part 113 is used for placing the dyeing liquid; labels attached to the first placement unit 111, the second placement unit 112, and the third placement unit 113; the first placing portion 111, the second placing portion 112, and the third placing portion 113 are connected to the multi-way valve 120, respectively.
It can be understood that the dyeing assembly 110 is provided with a placing frame, a first placing part 111, a second placing part 112 and a third placing part 113, wherein three fixing holes are arranged on the placing frame side by side, and the first placing part 111, the second placing part 112 and the third placing part 113 are respectively arranged through the three fixing holes, so as to fix the positions. The lysis solution has been placed to first placing portion 111, and the stationary liquid has been placed to second placing portion 112, and the staining solution has been placed to third placing portion 113 to corresponding label is respectively pasted at first placing portion 111, second placing portion 112 and third placing portion 113, and the reagent of placing in this suggestion every placing portion avoids taking the mistake. And the first placing portion 111, the second placing portion 112, and the third placing portion 113 are connected to the corresponding passages of the multi-passage valve 120.
After the cell capturing operation is completed, the target cell is stored in the cell staining chip 140, the multi-way valve 120 is adjusted, the passage between the first placing part 111 and the multi-way valve 120 and the passage between the multi-way valve 120 and the common liquid port 130 are opened, the lysate is conveyed to the common liquid port 130 from the multi-way valve 120 for buffering, the bubbles between the first placing part 111 and the multi-way valve 120 are removed, after the bubbles are eliminated, the passage of the multi-way valve 120 is adjusted, the passage between the common liquid port 130 and the cell staining chip 140 is opened, the lysate is conveyed to the cell staining chip 140 until the lysate overflows from the cell staining chip 140, the overflowing lysate is conveyed to the waste liquid barrel 150 from the cell staining chip 140, the red blood cell is lysed by the lysate, and the interference of the red blood cell on the subsequent counting of the number of the target cell is eliminated, and ensures that no bubbles remain in the cell staining chip 140.
Then, the multi-way valve 120 is adjusted, the passage between the second placing part 112 and the multi-way valve 120 and the passage between the multi-way valve 120 and the common liquid passage port 130 are opened, the stationary liquid is conveyed into the common liquid passage port 130 from the multi-way valve 120 and is buffered, bubbles between the second placing part 112 and the multi-way valve 120 are removed, after the bubbles are eliminated, the passage of the multi-way valve 120 is adjusted, the passage between the common liquid passage port 130 and the cell staining chip 140 is opened, the stationary liquid is conveyed into the cell staining chip 140 until the stationary liquid overflows from the cell staining chip 140, the overflowing stationary liquid is conveyed from the cell staining chip 140 to the waste liquid tank 150, the shape of the target cell is fixed by the stationary liquid, and no bubble residue is ensured in the cell staining chip 140.
Then, the multi-way valve 120 is adjusted, the passage between the third placing part 113 and the multi-way valve 120 and the passage between the multi-way valve 120 and the common liquid passage port 130 are opened, so that the staining solution is delivered into the common liquid passage port 130, the staining solution is delivered from the multi-way valve 120 to the common liquid passage port 130 for buffering, so that bubbles between the third placing part 113 and the multi-way valve 120 are removed, after the bubbles are eliminated, the passage of the multi-way valve 120 is adjusted, so that the passage between the common liquid passage port 130 and the cell staining chip 140 is opened, the staining solution is delivered into the cell staining chip 140 until the staining solution overflows from the cell staining chip 140, the overflowing staining solution is delivered from the cell staining chip 140 to the waste liquid tank 150, the target cell is stained by the staining solution, and no bubble residue is left in the cell staining chip 140.
It can be understood that three waste liquid barrels 150 may be provided, corresponding to the first placing part 111, the second placing part 112 and the third placing part 113, respectively, to prevent waste reagents from being mixed in the waste liquid barrels 150, thereby ensuring safety.
In some examples, as shown in fig. 1, the cell staining system 100 further includes: and a buffer solution feeding device 160 connected to the common liquid path port 130 for feeding a buffer solution to the common liquid path port 130.
It can be understood that the cell staining system 100 is further provided with a buffer solution conveying device 160, before the reagents in the first placing part 111, the second placing part 112 and the third placing part 113 are conveyed to the cell staining chip 140, the buffer solution conveying device 160 is required to convey the buffer solution to the cell staining chip 140 to clean the cell staining chip 140, so that reagent residues are avoided, the final staining effect is not affected, and the buffer solution is used as a cleaning agent, so that the influence on the target cells is reduced, the staining errors are avoided, and the accuracy of the subsequent determination of the number of the target cells according to the staining result is ensured. Specifically, the buffer solution is delivered to the common fluid path port 130, so that the reagents originally in the common fluid path port 130 are discharged from the cell staining chip 140 to the corresponding waste fluid bucket 150, the buffer solution is continuously delivered until the buffer solution overflows from the cell staining chip 140, and the overflowing buffer solution is delivered to the corresponding waste fluid bucket 150, so that the reagents originally in the channel of the cell staining chip 140 are completely discharged without bubbles.
Illustratively, the buffer may be PBS (phosphate buffer), have a salt balance, be adjusted for proper pH buffering, or be physiological saline.
In some examples, as shown in fig. 1, the buffer delivery device 160 includes: a storage part 161 connected to the common liquid path port 130 for storing the buffer liquid; a direction change valve 162 provided between the housing 161 and the common liquid passage port 130; and a pump body 163 connected to the selector valve 162.
It is understood that the buffer fluid feeding device 160 is provided with a container 161, a direction switching valve 162, and a pump body 163, wherein the container 161 is connected to the common fluid path port 130, the container 161 is for containing the buffer fluid, the direction switching valve 162 is provided between the container 161 and the common fluid path port 130, and the pump body 163 is connected to the direction switching valve 162. With this arrangement, the passage between the container 161 and the common liquid passage port 130 can be opened or closed by adjusting the passage of the switching valve 162, and the pump body 163 supplies power to pump the reagent in the staining module 110 into the cell staining chip 140 or pump the buffer in the container 161 into the cell staining chip 140 in cooperation with the switching valve 162 and the multi-passage valve 120.
Illustratively, the pump body 163 can be a plunger pump, and the plunger pump has the characteristics of high rated pressure, compact structure, high efficiency, convenient flow regulation and the like.
In some examples, as shown in fig. 1, the common fluid port 130 is provided with a spiral section 131.
It can be understood that the end of the common liquid path port 130 for retaining liquid is provided with the spiral section 131, so that the deposition phenomenon of the reagent in the common liquid path port 130 is reduced, and the spiral section 131 increases the volume of the common liquid path port 130, thereby improving the temporary storage capacity.
In some examples, the cell staining system 100 further comprises: and a controller connected to the pump body 163 for controlling the speed at which the pump body 163 extracts the reagent from the staining module 110 or the speed at which the buffer solution is extracted from the storage part 161, and controlling the flow rate of the reagent to be transferred to the cell staining chip 140.
It is understood that the cell staining system 100 is further provided with a controller, which is connected to the pump body 163, and the power of the pump body 163 is controlled by the controller to adjust the speed of extracting the reagent from the staining assembly 110 or the speed of extracting the buffer solution from the container 161 and the flow rate of the reagent to the cell staining chip 140 according to the actual demand on site. Ensuring the reliability and stability of the cell staining system 100.
In some examples, as shown in fig. 2, according to a second aspect of the embodiments of the present application, a method for controlling a cell staining system 100 is provided, for controlling the cell staining system 100 according to any of the above technical solutions, where the cell staining system 100 includes: the buffer solution delivery device 160 and the waste liquid barrel 150, the method comprising:
s101: and receiving a control instruction. It is understood that the cell capture staining system may be provided with a control device, and the control device may issue corresponding instructions according to the steps performed by the cell staining operation, so that the controller adjusts the power of the pump body 163 to control the switching of the passages of the multi-way valve 120 and the switching of the passages of the reversing valve 162.
S102: the reagents of the staining module 110 and the buffer solution of the buffer solution delivering device 160 are extracted and delivered to the cell staining chip 140 according to the operation contents indicated by the control command. It can be understood that, after the capturing operation of the target cells is completed, the control device sends control instructions to the respective components in the cell staining system 100, the pump 163 adjusts the power to adjust the speed of extracting the reagent from the staining assembly 110 or the speed of extracting the buffer solution from the accommodating part 161, the path of the multi-path valve 120 is switched to adjust the transmission routes of the reagent in the first, second, and third accommodating parts 111, 112, and 113 and the buffer solution in the accommodating part 161, and the path of the direction-changing valve 162 is controlled to adjust whether the buffer solution in the accommodating part 161 flows into the common liquid path port 130, so as to complete the cell staining operation in cooperation with the cell staining chip 140. By using the method, bubbles in the cell staining chip 140 can be effectively avoided, the interference of the bubbles on cell staining operation can be reduced as much as possible, the cell staining chip 140 can accurately stain target cells, the number of the target cells can be determined accurately according to staining results, the current state of illness of a patient can be accurately judged, and accurate reference can be provided for targeted therapy.
In some examples, the steps of extracting the reagent of the staining assembly 110 and extracting the buffer of the buffer delivering device 160 to be delivered to the cell staining chip 140 according to the work content indicated by the control command include: in the case that the work content includes washing, the buffer solution is pumped and filled into the cell staining chip 140, and the buffer solution overflowing the cell staining chip 140 is discharged into the waste liquid tank 150; under the condition that the working content includes lysis, controlling the pump body 163 to extract lysis solution to fill the cell staining chip 140, and discharging lysis solution overflowing the cell staining chip 140 into the waste liquid barrel 150; controlling the pump 163 to pump a fixing solution to fill the cell staining chip 140 and discharge the fixing solution overflowing the cell staining chip 140 into the waste liquid tank 150 when the operation content includes fixing; in the case where the operation contents include the staining, the pump 163 is controlled to pump the staining solution to fill the cell staining chip 140, and the staining solution overflowing the cell staining chip 140 is discharged to the waste liquid tank 150.
It will be appreciated that the reagent remains in the cell staining system 100 after each use of the reagent, requiring a cleaning operation to remove the remaining reagent. When the work content indicated by the control instruction is cleaning, the multi-way valve 120 is adjusted, all the passages between the staining component 110 and the common liquid passage port 130 are closed, the reversing valve 162 is adjusted, the passages between the accommodating part 161 and the common liquid passage port 130 are opened, so that the buffer solution in the accommodating part 161 is pumped into the common liquid passage port 130 through the pump body 163, the buffer solution is conveyed into the cell staining chip 140 from the common liquid passage port 130 after the residual reagent in the buffer solution is flushed through the common liquid passage port 130, the passages of the cell staining chip 140 are cleaned until the buffer solution overflows from the cell staining chip 140, the overflowing buffer solution is conveyed into the corresponding waste liquid barrel 150, the residual reagent is discharged through the buffer solution, the influence of the residual reagent on the subsequently conveyed reagent is avoided, and the staining reliability is ensured.
When the work content indicated by the control instruction is cracking, the multi-way valve 120 is adjusted to open a passage between the first placing portion 111 and the common liquid path port 130, the reversing valve 162 is adjusted to open a passage between the common liquid path port 130 and the reversing valve 162, so that the lysate in the first placing portion 111 is pumped into the common liquid path port 130 through the pump body 163, after the lysate is temporarily stored in the common liquid path port 130, the multi-way valve 120 is adjusted again to open a passage between the common liquid path port 130 and the cell staining chip 140, so that the lysate is conveyed from the common liquid path port 130 to the cell staining chip 140 until the lysate overflows from the cell staining chip 140, the overflowing lysate is conveyed to the corresponding waste liquid barrel 150, and no bubble interference is ensured in the cracking work process.
After completion of the lysis operation, the washing operation is performed again, and the lysate remaining in the cell-stained chip 140 is removed by the buffer solution. When the work content indicated by the control instruction is fixed, the multi-way valve 120 is adjusted to open the passage between the second placing portion 112 and the common liquid path port 130, the reversing valve 162 is adjusted to open the passage between the common liquid path port 130 and the reversing valve 162, so that the stationary liquid in the second placing portion 112 is pumped into the common liquid path port 130 through the pump body 163, after the stationary liquid is temporarily stored in the common liquid path port 130, the multi-way valve 120 is adjusted again to open the passage between the common liquid path port 130 and the cell staining chip 140, so that the stationary liquid is conveyed from the common liquid path port 130 to the cell staining chip 140 until the stationary liquid overflows from the cell staining chip 140, and the overflowing stationary liquid is conveyed to the corresponding waste liquid barrel 150, thereby ensuring that no bubble interference exists in the fixing work process.
After the fixing operation is completed, the washing operation is performed again, and the fixing solution remaining in the cell-stained chip 140 is removed by the buffer solution. When the work content indicated by the control instruction is dyeing, the multi-way valve 120 is adjusted to open the passage between the third placing part 113 and the common liquid path port 130, the reversing valve 162 is adjusted to open the passage between the common liquid path port 130 and the reversing valve 162, so that the fixed liquid in the third placing part 113 is pumped into the common liquid path port 130 through the pump body 163, after the dyeing liquid is temporarily stored in the common liquid path port 130, the multi-way valve 120 is adjusted again to open the passage between the common liquid path port 130 and the cell dyeing chip 140, so that the dyeing liquid is conveyed from the common liquid path port 130 to the cell dyeing chip 140 until the dyeing liquid overflows from the cell dyeing chip 140, the overflowing dyeing liquid is conveyed to the corresponding waste liquid barrel 150, and no bubble interference is generated in the dyeing work process. The cell staining chip 140 can accurately stain the target cells, so that the number of the target cells is determined to be accurate according to the staining result, the current state of illness of the patient can be accurately judged, and accurate reference is provided for targeted treatment.
As shown in fig. 3, according to a third aspect of the embodiments of the present application, a computer-readable storage medium 401 is provided, the computer-readable storage medium 401 stores a computer program 402, and the method for controlling the cell staining system 100 according to any of the above-mentioned embodiments is implemented.
The computer-readable storage medium 401 provided in the embodiment of the present application, after receiving the control instruction, acquires the rotation speed information of the driving component, extracts the reagent of the staining component 110 and the buffer solution of the buffer solution extracting and delivering device 160 according to the work content indicated by the control instruction, and delivers the reagent and the buffer solution to the cell staining chip 140, so as to complete the staining operation on the target cell, effectively avoid the existence of air bubbles in the cell staining chip 140, reduce the interference of the air bubbles on the cell staining operation as much as possible, and enable the cell staining chip 140 to stain the target cell accurately, thereby determining the number of the target cell accurately according to the staining result, and being capable of accurately determining the current condition of the patient, and providing an accurate reference for the targeted therapy.
Based on such understanding, the technical solution of the present application may be embodied in the form of a software product, which may be stored in a non-volatile storage medium (which may be a CD-ROM, a usb disk, a removable hard disk, etc.), and includes several instructions for enabling a computer device (which may be a personal computer, a server, or a network device, etc.) to execute the method according to the implementation scenarios of the present application.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "left", "right", "front", "rear", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the referred device or unit must have a specific direction, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
In the description herein, the description of the terms "one embodiment," "some embodiments," "specific embodiments," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A cell staining system, comprising:
a staining component, wherein the staining component is provided with reagents required by cell staining;
the test tube assembly is connected with the multi-way valve;
a common fluid port connected to the multi-way valve, the common fluid port being for retaining the reagent in the cuvette assembly;
and the cell staining chip is connected with the multi-way valve.
2. The cell staining system of claim 1, further comprising:
and the waste liquid barrel is connected with the cell staining chip.
3. The cell staining system of claim 1, wherein the staining component comprises:
the placing frame is provided with a plurality of fixing holes;
the first placing part penetrates through the fixing hole, and lysis solution is placed on the first placing part;
the second placing part penetrates through the fixing hole, and fixing liquid is placed in the second placing part;
the third placing part penetrates through the fixing hole, and the dyeing solution is placed on the third placing part; labels attached to the first, second, and third placement portions;
the first placing portion, the second placing portion and the third placing portion are connected to the multi-way valve respectively.
4. The cell staining system of claim 1, further comprising:
and the buffer solution conveying device is connected to the common liquid path port and is used for conveying the buffer solution to the common liquid path port.
5. The cell staining system of claim 4, wherein the buffer delivery device comprises:
a housing part connected to the common liquid path port for housing the buffer liquid;
a direction change valve provided between the accommodating portion and the common liquid path port;
and the pump body is connected with the reversing valve.
6. The cell staining system of claim 1,
and the port of the common liquid path is provided with a spiral section.
7. The cell staining system of claim 5, further comprising:
and the controller is connected to the pump body and is used for controlling the speed of the pump body for extracting the reagent from the staining assembly or the buffer solution from the accommodating part and controlling the flow rate of the reagent conveyed to the cell staining chip.
8. A control method of a cell staining system for controlling the cell staining system according to any one of claims 1 to 7, the cell staining system comprising: buffer fluid conveyor with waste liquid bucket, its characterized in that includes:
receiving a control instruction;
extracting the reagent of the staining component and the buffer of the buffer conveying device to convey into the cell staining chip according to the work content indicated by the control instruction.
9. The method for controlling a cell staining system according to claim 8, wherein the steps of extracting the reagent of the staining component and extracting the buffer of the buffer delivery device for delivery into the cell staining chip according to the work content indicated by the control instruction comprise:
in the case that the work content comprises washing, pumping the buffer solution to be filled into the cell staining chip, and discharging the buffer solution overflowing the cell staining chip into the waste liquid barrel;
under the condition that the working content comprises cracking, controlling the pump body to extract a cracking solution to fill the cell staining chip, and discharging the cracking solution overflowing the cell staining chip into the waste liquid barrel;
under the condition that the work content comprises fixation, controlling the pump body to extract fixing liquid to fill the cell staining chip, and discharging the fixing liquid overflowing the cell staining chip into the waste liquid barrel;
and under the condition that the work content comprises dyeing, controlling the pump body to pump the dyeing liquid to fill the cell dyeing chip, and discharging the dyeing liquid overflowing the cell dyeing chip into the waste liquid barrel.
10. A computer-readable storage medium having stored thereon a computer program, characterized in that: the computer program, when executed by a processor, implements a method of controlling a cell staining system according to claim 8 or 9.
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