CN114437977B - Lactobacillus reuteri, feed and application thereof - Google Patents
Lactobacillus reuteri, feed and application thereof Download PDFInfo
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- 229940001882 lactobacillus reuteri Drugs 0.000 title claims abstract description 66
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- 238000004321 preservation Methods 0.000 claims abstract 2
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- 235000013601 eggs Nutrition 0.000 claims description 21
- 238000000855 fermentation Methods 0.000 claims description 18
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- 241000287828 Gallus gallus Species 0.000 claims description 13
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- 239000008280 blood Substances 0.000 claims description 11
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- 102100036475 Alanine aminotransferase 1 Human genes 0.000 claims description 9
- 108010082126 Alanine transaminase Proteins 0.000 claims description 9
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- 241000269335 Ambystoma laterale x Ambystoma jeffersonianum Species 0.000 description 1
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- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses lactobacillus reuteri with a function of reducing cholesterol in egg yolk and application thereof. The lactobacillus reuteri DBN-SKL01 has the strain preservation number of CGMCC No.19491 in the common microorganism center of China Committee for culture Collection of microorganisms, and has the effect of obviously reducing the cholesterol content in egg yolk when being applied to laying hen feed.
Description
Technical Field
The invention belongs to the field of feeding microecologics, and relates to lactobacillus reuteri with a function of reducing cholesterol in egg yolk and application thereof.
Background
The eggs contain high-quality protein, essential amino acids and various trace elements, have high nutritive value, and the quality of eggs and the shelf life of the eggs directly influence human health and cultivation benefits.
Along with the continuous rising of the morbidity and mortality of cardiovascular and cerebrovascular diseases such as atherosclerosis and coronary heart disease, people with the need of strictly controlling cholesterol intake such as hyperlipidemia and high cholesterol have urgent demands on low-cholesterol and high-quality egg products, and the development of low-cholesterol eggs has great application value. In recent years, the application of the microecological preparation in the laying hen breeding is more extensive, and Mohan et al report that the composite probiotics of lactobacillus acidophilus, lactobacillus casei and bifidobacterium are added into the laying hen diet, so that the egg yolk cholesterol content is obviously reduced.
The invention provides lactobacillus reuteri which can reduce the cholesterol content in egg yolk, deeply discusses the influence of the lactobacillus reuteri on egg quality, blood biochemical indexes and the cholesterol content in egg yolk, and provides practical guidance for the application of the lactobacillus reuteri in laying hen breeding and functional food development.
Disclosure of Invention
In order to solve the defects and shortcomings in the prior art, the invention aims to provide lactobacillus reuteri and application thereof.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
lactobacillus reuteri, named DBN-SKL01, was deposited in the China general microbiological culture collection center, abbreviated as CGMCC, address: the collection number of the national institute of microbiology, national academy of sciences, no. 3, north Chen West Lu 1, chao yang, beijing, is: cgmccno.19491.
The invention also provides a microecological preparation containing the lactobacillus reuteri DBN-SKL 01.
The invention also provides a feed containing the lactobacillus reuteri DBN-SKL 01.
Further, the viable count of the Lactobacillus reuteri in the feed is 1×10 9 ～5×10 11 cfu/kg, preferably 5X 10 10 cfu/kg.
The invention also provides application of the lactobacillus reuteri DBN-SKL01 in chicken breeding.
The invention also provides application of the lactobacillus reuteri DBN-SKL01 in improving alanine aminotransferase, serum total cholesterol, high-density lipoprotein cholesterol and serum triglyceride in chicken blood;
in particular to a method for preparing lactobacillus reuteri DBN-SKL01 into medicines or feeds for improving alanine aminotransferase, serum total cholesterol, high-density lipoprotein cholesterol and serum triglyceride in chicken blood.
The invention also provides application of the lactobacillus reuteri DBN-SKL01 in improving chicken egg quality.
The invention also provides an application of the lactobacillus reuteri DBN-SKL01 in reducing the cholesterol content of eggs;
in particular to a method for preparing the lactobacillus reuteri into medicines or feeds for reducing the cholesterol content of eggs.
The invention also provides a fermentation process of the lactobacillus reuteri DBN-SKL01, which comprises the following steps of fermentation by the following culture medium and fermentation conditions:
the seed culture medium consists of: glucose 5g/L, peptone 10g/L, yeast extract 5g/L, beef extract 5g/L, sodium chloride 5g/L, calcium carbonate 2.5g/L, pH6.5-7.0, sterilizing at 121deg.C for 30min;
the fermentation medium consists of: corn starch: molasses (1:1) 15g/L, soybean meal 20g/L, orange peel extract 15g/L, diammonium hydrogen citrate 2.0g/L, tween 80 1.0ml/L, sodium acetate 5.0g/L, dipotassium hydrogen phosphate 2.0g/L, magnesium sulfate 0.58g/L, manganese sulfate 0.25g/L, and autoclaving at 121deg.C for 30min;
fermentation conditions: inoculum size 10%, temperature: 37 ℃, liquid filling coefficient: 0.5-0.8, tank pressure: 0.03-0.05MPa, maintaining pH at 6.5 during fermentation, stirring at 50rpm, and fermenting to obtain final viable count: 6.42 to 7.84 multiplied by 10 9 cfu/ml。
Compared with the prior art, the invention has the beneficial effects that:
the lactobacillus reuteri has the beneficial effects of high safety and capability of remarkably reducing the cholesterol content in egg yolk. Experiments prove that the content of cholesterol in egg yolk of the eggs can be obviously reduced, and the content of alanine aminotransferase, serum total cholesterol, high-density lipoprotein cholesterol, serum triglyceride and cholesterol in egg yolk in chicken blood can be obviously reduced.
Drawings
FIG. 1 shows colony morphology of Lactobacillus reuteri DBN-SKL 01;
FIG. 2 shows gastric acid resistance of Lactobacillus reuteri DBN-SKL 01;
FIG. 3 shows the bile salt tolerance of Lactobacillus reuteri DBN-SKL 01;
FIG. 4 shows the growth curve of Lactobacillus reuteri DBN-SKL 01;
Detailed Description
The following examples are illustrative of the invention and are not intended to limit the scope of the invention. The experimental methods used in the following examples are conventional methods unless otherwise specified. Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified.
Example 1: screening of Lactobacillus reuteri DBN-SKL01
A strain of Lactobacillus reuteri DBN-SKL01 is isolated and screened from the ileum of healthy piglets, the colony morphology is shown in figure 1, and the 16sRNA sequencing result is as follows:
identification of 16s amplified region:
as shown in fig. 2 and 3, lactobacillus reuteri DBN-SKL01 has good acid resistance, compared with normal pH, the treatment with pH 4.0 of simulated gastric acid solution only slows down the growth rate, but is not lethal to it; the survival rate of the simulated gastric acid solution after being treated for 6 hours with the pH value of 2.0 is more than 95 percent; the resistance to 0.3% of bile salts is good, and the survival rate of the treated bile salts is more than 95% after 8 hours; the survival rate of the treatment for 8 hours with 0.5 percent of bile salt concentration can be more than 93 percent. This shows that lactobacillus reuteri DBN-JP has better acid and bile salt resistance and has potential for developing as feed probiotics.
The fermentation process of lactobacillus reuteri DBN-SKL01 1000L fermentation tank is as follows:
seed medium (g/L): glucose 5, peptone 10, yeast extract 5, beef extract 5, sodium chloride 5, calcium carbonate 2.5, pH6.5-7.0, and sterilizing at 121deg.C for 30min.
Fermentation medium (g/L): corn starch: molasses (1:1) 15, soya bean meal 20, orange peel extract 15, diammonium hydrogen citrate [ (NH) 4 ) 2 HC 6 H 5 O 7 ]2.0 Tween 80 1.0mL/L, sodium acetate (CH 3 COONa·3H 2 O) 5.0, dipotassium hydrogen phosphate (K) 2 HPO 4 ·3H 2 O) 2.0, magnesium sulfate (MgSO) 4 ·7H 2 O) 0.58, manganese sulfate (MnSO) 4 ·H 2 O) 0.25, 121℃for 30min.
Temperature: 37 ℃, liquid filling coefficient: 0.5-0.8, tank pressure: 0.03-0.05MPa, the process automatically regulates the pH value to 6.5, the inoculum size is 10%, the stirring speed is 50rpm, and as shown in figure 4, the number of the viable bacteria is: 6.42 to 7.84 multiplied by 10 9 cfu/ml。
EXAMPLE 2 preparation of Lactobacillus reuteri lyophilized powder
Fermentation medium: MRS medium;
culture conditions: anaerobic culture at 35-40deg.C for 40-50 hr; centrifuging the fermentation liquor at 4000rpm for 30min to obtain bacterial mud, and adding a freeze-drying protective agent to prepare bacterial suspension with the same concentration as the fermentation liquor before centrifugation.
Pouring the bacterial suspension into a material drying tray of a freeze dryer, pre-freezing for 2-3 hours, and when the machine reaches the second-stage refrigeration, vacuumizing to start freeze drying, wherein the temperature in a cold well of the freeze dryer is-80 ℃ and the freeze drying time is 18-24 hours. The freeze-drying protective agent comprises the following components: 10% of skimmed milk powder, 1.5% of sodium glutamate, 0.5% of L-cysteine, 2% of lactose and 10% of dextrin. The technology is a conventional technology in the industry.
In the following examples, the basic diet formula of the laying hen is shown in Table 1
Table 1 basal diet levels and nutritional composition
The detection method of the egg quality related index in the following embodiment comprises the following steps: egg shape index, eggshell color, eggshell strength, egg white height, haugh unit, egg yolk color, eggshell thickness were measured using a multifunctional egg quality analysis meter.
The method for detecting the cholesterol content in the egg yolk in the following examples comprises the following steps: taking a fresh egg yolk sample after an egg quality test, weighing 2.5g, putting into a 25mL volumetric flask, adding normal saline to fix the volume to a scale mark, shaking uniformly, preparing 10% egg yolk diluent, and measuring the content of cholesterol in egg yolk by using a cholesterol kit, wherein the kit is purchased from Nanjing built bioengineering institute.
The measurement method of serum parameter indexes in the following examples is as follows: the test chicken is subjected to vein blood sampling under the wing, the test chicken is placed obliquely at room temperature for 20min, and after centrifugation for 15min at 3 000r/min, serum is separated, and after the serum is split, the test chicken is stored at-20 ℃ for measuring the index below. The serum biochemical index is determined by full-automatic biochemical analyzer, such as total protein, globulin, albumin, aspartic acid aminotransferase, alanine aminotransferase, serum total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol and triglyceride.
All data in the following examples were analyzed by One-Way ANOVA using IBM SPASS Statistics statistical software, multiple comparisons were made using Duncan's method, and the results were expressed as mean ± standard error, and difference significance was judged as P < 0.05.
Example 3 influence of Lactobacillus reuteri addition on the cholesterol content in egg yolk
The 800 feathers of healthy laying hens in the laying peak period of the laying hens are selected and randomly divided into 4 groups of 4 repeats each, and 50 repeats each. Group A: control group, only basal diet was added; group B: adding lactobacillus reuteri DBN-SKL01 viable count of 5×10 9 cfu/kg basal ration; group C: adding lactobacillus reuteri DBN-SKL01 viable count of 5×10 10 cfu/kg basal ration; group D: adding a royLactobacillus gasseri DBN-SKL01 viable count 5×10 11 cfu/kg basal ration; 3 days of pre-feeding and 49 days of formal period. Immunization and daily management are carried out according to the conventional management program of the test chicken farm, the test is carried out in the same house, and the feeding mode is four-layer ladder type free-range feeding, namely 8:00 am and 4:00 pm, and the feeding is carried out twice a day. The special fixed people feed, eat freely, the automatic drinking machine drinks water, the production condition of the chicken flock is observed at any time, and the diseases are found to be treated in time.
Analysis of cholesterol content in egg yolk: after the test, 20 eggs were repeatedly taken each time, and the cholesterol content of the egg yolk was analyzed using an ELISA kit.
TABLE 2 Effect of addition of Lactobacillus reuteri DBN-SKL01 at different concentrations on cholesterol content in egg yolk
Compared with the control group, the feed additive for the laying hen is 5×10 10 cfu/kg lactobacillus reuteri DBN-SKL01 can obviously reduce the cholesterol content (P is less than 0.05) in egg yolk; the addition amount is 5×10 11 cfu/kg and 5X 10 10 The difference in cfu/kg effect on cholesterol in eggs was insignificant (P > 0.05).
Example 4 influence of Lactobacillus reuteri addition on the cholesterol content in egg yolk and blood Biochemical index
The 800 feathers of healthy laying hens in the laying peak period of the laying hens are selected and randomly divided into 4 groups of 4 repeats each, and 50 repeats each. Group A: adding lactobacillus reuteri DBN-SKL01 viable count of 1×10 10 cfu/kg basal ration; group B: adding lactobacillus reuteri DBN-SKL01 viable count of 5×10 10 cfu/kg basal ration; group C: adding lactobacillus reuteri DBN-SKL01 viable count of 9×10 10 cfu/kg basal ration; group D: control group, only basal diet was added; 3 days of pre-feeding and 49 days of formal period. Immunization and daily management are carried out according to the conventional management program of the test chicken farm, the test is carried out in the same house, and the feeding mode is four-layer ladder type free-range feeding, namely 8:00 am and 4:00 pm, and the feeding is carried out twice a day. Fixed special feeding and free collectionEating, drinking water by an automatic drinking machine, observing the production condition of chicken flocks at any time, and finding out the disease to treat in time.
After the test, 20 eggs are repeatedly taken each time, and the relevant indexes of the egg quality and the total cholesterol content in the yolk and the serum are measured.
TABLE 3 Effect of adding Lactobacillus reuteri DBN-SKL01 at different concentrations on egg quality
Compared with the control group, the content of lactobacillus reuteri DBN-SKL01 in the feed of the laying hen is 1.0X10 10 cfu/kg～9.0×10 10 At cfu/kg, the effect on indexes such as egg shape index, eggshell color, eggshell strength, egg white height, half unit, yolk color, eggshell thickness and the like of the egg is not obvious (P is more than 0.05).
TABLE 4 influence of different addition amounts on biochemical indices of blood of egg-laying hens and cholesterol in egg yolk
Compared with the control group, the content of lactobacillus reuteri DBN-SKL01 in the feed of the laying hen is 1.0X10 10 cfu/kg～9.0×10 10 At cfu/kg, the total protein, albumin, globulin, aspartic acid aminotransferase and low density lipoprotein cholesterol in the blood of the laying hen are not obviously influenced (P is more than 0.05); but remarkably reduces the content of alanine aminotransferase, serum total cholesterol, high density lipoprotein cholesterol, serum triglyceride and cholesterol in egg yolk (P < 0.05).
When the content of lactobacillus reuteri DBN-SKL01 in the feed of the laying hen is 1.0x10 10 compared with the control group, the cfu/kg has the advantages that the alanine aminotransferase in the blood of the laying hens is obviously reduced by 7.percent (P is less than 0.05),The total cholesterol in serum is obviously reduced by 11.23 percent (P is less than 0.05), the high-density lipoprotein cholesterol is reduced by 12.20 percent (P is less than 0.05), the triglyceride in serum is obviously reduced by 23.90 percent (P is less than 0.05), and the cholesterol content in egg yolk is obviously reduced by 12.14 percent (P is less than 0.05); the content of lactobacillus reuteri DBN-SKL01 in the feed for laying hens is 5.0 multiplied by 10 10 cfu/kg group and Lactobacillus reuteri DBN-SKL01 content of 9.0X10 10 compared with cfu/kg group, the total cholesterol of serum is obviously higher than that of the other two groups (P < 0.05), and the difference of other indexes is not obvious (P > 0.05).
When the content of lactobacillus reuteri DBN-SKL01 in the feed of the laying hen is 5.0X10% 10 When cfu/kg, compared with a control group, alanine aminotransferase in the blood of the laying hen is obviously reduced by 9.44 percent (P is less than 0.05), serum total cholesterol is obviously reduced by 17.11 percent (P is less than 0.05), high-density lipoprotein cholesterol is reduced by 20.33 percent (P is less than 0.05), serum triglyceride is obviously reduced by 20.09 percent (P is less than 0.05), and the content of cholesterol in egg yolk is obviously reduced by 13.69 percent (P is less than 0.05); the content of lactobacillus reuteri DBN-SKL01 in the feed for laying hens is 1.0x10 10 compared with cfu/kg group, the total cholesterol content of serum is obviously reduced by 6.63 percent (P is less than 0.05), and other indexes are not obviously different; the content of DBN-SKL01 with lactobacillus reuteri is 9.0X10 10 The difference of each index is not obvious (P is more than 0.05) in cfu/kg group.
When the content of lactobacillus reuteri DBN-SKL01 in the feed of the laying hen is 9.0x10 10 When cfu/kg, compared with a control group, alanine aminotransferase in the blood of the laying hen is obviously reduced by 8.25 percent (P is less than 0.05), serum total cholesterol is obviously reduced by 21.39 percent (P is less than 0.05), high-density lipoprotein cholesterol is reduced by 15.45 percent (P is more than 0.05), serum triglyceride is obviously reduced by 25.02 percent (P is less than 0.05), and the content of cholesterol in egg yolk is obviously reduced by 13.95 percent (P is less than 0.05); the content of lactobacillus reuteri DBN-SKL01 in the feed for laying hens is 1.0x10 10 compared with cfu/kg group, the total cholesterol content of serum is obviously reduced by 11.45 percent (P is less than 0.05), and other indexes are not obviously different; the content of DBN-SKL01 with lactobacillus reuteri is 5.0X10 10 The difference of each index is not obvious (P is more than 0.05) in cfu/kg group.
In conclusion, lactobacillus reuteri DBN-SKL01 content is 1.0X1 in the feed of the laying hen0 10 cfu/kg～9.0×10 10 cfu/kg can effectively reduce the cholesterol content in egg yolk, comprehensively consider the total cholesterol content in egg yolk serum, high density lipoprotein cholesterol, the index of the cholesterol content in egg yolk and the use cost, and the optimal adding amount of lactobacillus reuteri DBN-SKL01 in the feed of the egg yolk is 5.0x10 10 cfu/kg。
The foregoing is merely a preferred embodiment of the present invention, and it should be noted that it will be apparent to those skilled in the art that several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the scope of the invention.
Sequence listing
<110> Beijing blue and Wei industry science and technology Co., ltd
Beijing Dabei Agricultural Technology Group Co., Ltd.
<120> Lactobacillus reuteri, feed and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1422
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 1
ggttaggcca ccgactttgg gcgttacaaa ctcccatggt gtgacgggcg gtgtgtacaa 60
ggcccgggaa cgtattcacc gcggcatgct gatccgcgat tactagcgat tccgacttcg 120
tgtaggcgag ttgcagccta cagtccgaac tgagaacggc tttaagagat tagcttactc 180
tcgcgagctt gcgactcgtt gtaccgtcca ttgtagcacg tgtgtagccc aggtcataag 240
gggcatgatg atctgacgtc gtccccacct tcctccggtt tgtcaccggc agtctcacta 300
gagtgcccaa cttaatgctg gcaactagta acaagggttg cgctcgttgc gggacttaac 360
ccaacatctc acgacacgag ctgacgacga ccatgcacca cctgtcattg cgtccccgaa 420
gggaacgcct tatctctaag gttagcgcaa gatgtcaaga cctggtaagg ttcttcgcgt 480
agcttcgaat taaaccacat gctccaccgc ttgtgcgggc ccccgtcaat tcctttgagt 540
ttcaaccttg cggtcgtact ccccaggcgg agtgcttaat gcgttagctc cggcactgaa 600
gggcggaaac cctccaacac ctagcactca tcgtttacgg catggactac cagggtatct 660
aatcctgttc gctacccatg ctttcgagcc tcagcgtcag ttgcagacca gacagccgcc 720
ttcgccactg gtgttcttcc atatatctac gcattccacc gctacacatg gagttccact 780
gtcctcttct gcactcaagt cgcccggttt ccgatgcact tcttcggtta agccgaaggc 840
tttcacatca gacctaagca accgcctgcg ctcgctttac gcccaataaa tccggataac 900
gcttgccacc tacgtattac cgcggctgct ggcacgtagt tagccgtgac tttctggttg 960
gataccgtca ctgcgtgaac agttactctc acgcacgttc ttctccaaca acagagcttt 1020
acgagccgaa acccttcttc actcacgcgg tgttgctcca tcaggcttgc gcccattgtg 1080
gaagattccc tactgctgcc tcccgtagga gtatggaccg tgtctcagtt ccattgtggc 1140
cgatcagtct ctcaactcgg ctatgcatca tcgccttggt aagccgttac cttaccaact 1200
agctaatgca ccgcaggtcc atcccagagt gatagccaaa gccatctttc aaacaaaagc 1260
catgtggctt ttgttgttat gcggtattag catctgtttc caaatgttat cccccgctcc 1320
ggggcaggtt acctacgtgt tactcacccg tccgccactc actggtgatc catcgtcaat 1380
caggtgcaag caccatcaat cagttgggcc agtgcgtacg ac 1422
Claims (9)
1. Lactobacillus reuteri (Lactobacillus reuteri) is characterized in that the lactobacillus reuteri has a strain preservation number of CGMCC No.19491.
2. A microbial preparation comprising lactobacillus reuteri according to claim 1.
3. A feed comprising lactobacillus reuteri according to claim 1.
4. A feed according to claim 3, wherein the viable count of lactobacillus reuteri in the feed is 1 x 10 9 ～5×10 11 cfu/kg。
5. The feed as claimed in claim 4, wherein the viable count of lactobacillus reuteri in the feed is 5×10 10 cfu/kg。
6. Use of lactobacillus reuteri as claimed in claim 1 in chicken farming.
7. Use of lactobacillus reuteri according to claim 1 for the preparation of a medicament or feed for improving alanine aminotransferase, serum total cholesterol, high density lipoprotein cholesterol, serum triglycerides in chicken blood.
8. Use of lactobacillus reuteri according to claim 1 for the preparation of a medicament or feed for improving the cholesterol level in eggs.
9. The fermentation method of lactobacillus reuteri according to claim 1, wherein the fermentation is performed by the following medium and fermentation conditions:
the seed culture medium consists of: glucose 5g/L, peptone 10g/L, yeast extract 5g/L, beef extract 5g/L, sodium chloride 5g/L, calcium carbonate 2.5g/L, pH6.5-7.0, sterilizing at 121deg.C for 30min;
the fermentation medium consists of: corn starch and molasses total 15g/L, soybean meal 20g/L, orange peel extract 15g/L, diammonium hydrogen citrate 2.0g/L, tween 80 1.0ml/L, sodium acetate 5.0g/L, dipotassium hydrogen phosphate 2.0g/L, magnesium sulfate 0.58g/L, manganese sulfate 0.25g/L, and autoclaving at 121deg.C for 30min;
the corn starch and the molasses are prepared according to the mass ratio of 1:1;
fermentation conditions: inoculum size 10%, temperature: 37 ℃, liquid filling coefficient: 0.5-0.8, tank pressure: 0.03-0.05MPa, maintaining pH at 6.5 during fermentation, stirring at 50rpm, and fermenting to obtain final viable count: 6.42 to 7.84 multiplied by 10 9 cfu/ml。
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20080105846A (en) * | 2007-06-01 | 2008-12-04 | 신현길 | Lactic-acid bacterium or mixture thereof having the effect on reducing cholesterol in blood |
| CN110016443A (en) * | 2019-04-02 | 2019-07-16 | 山东大学 | A kind of lactobacillus reuteri and its application in Se-enriched egg production |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20080105846A (en) * | 2007-06-01 | 2008-12-04 | 신현길 | Lactic-acid bacterium or mixture thereof having the effect on reducing cholesterol in blood |
| CN110016443A (en) * | 2019-04-02 | 2019-07-16 | 山东大学 | A kind of lactobacillus reuteri and its application in Se-enriched egg production |
Non-Patent Citations (2)
| Title |
|---|
| Effect of supplementation of yeast with bacteriocin and Lactobacillus culture on growth performance, cecal fermentation, microbiota composition, and blood characteristics in broiler chickens;C. Y. Chen, et al;Anim Biosci;第30卷(第2期);第211-220页 * |
| 益生菌降血脂作用及机制研究进展;黄文丽等;工业微生物;第48卷(第4期);第64-65页 * |
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