CN114431135B - Breeding method of resistant starch and anthocyanin polymerized potatoes - Google Patents
Breeding method of resistant starch and anthocyanin polymerized potatoes Download PDFInfo
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- CN114431135B CN114431135B CN202210136586.9A CN202210136586A CN114431135B CN 114431135 B CN114431135 B CN 114431135B CN 202210136586 A CN202210136586 A CN 202210136586A CN 114431135 B CN114431135 B CN 114431135B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
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Abstract
The invention discloses a breeding method of resistant starch and anthocyanin polymerized potatoes, which comprises the steps of taking purple skin purple flesh potatoes as male parents and purple skin yellow flesh potatoes as female parents to obtain F through hybridization 1 Seeds; f 1 Harvesting small potatoes, reserving purple seed coat tubers, cutting into two pieces, selecting one piece of purple potato pulp, selecting corresponding small potatoes with RS2 more than or equal to the parent A and RS3 more than or equal to the parent B, and obtaining S 1 ；S 1 Observing the homozygous stable strain with purple skin and purple meat of the seed skin and selecting and reserving clone S with RS2 more than or equal to parent A and RS3 more than or equal to parent B 2 ；S 2 Selecting and reserving clone S with double high of RS2 more than or equal to 45 percent and RS3 more than or equal to 5 percent 3 ；S 3 Selecting and retaining 'double high-one low' clone S with reducing sugar content less than or equal to that of parents 4 (ii) a Finally breeding the potato with polymerized resistant starch and anthocyanin. The nutritional compound potato obtained by the breeding method has fresh eating and puffing functions, is expected to develop richer functional leisure food, and is helpful for quality improvement and efficiency improvement of future villages.
Description
Technical Field
The invention relates to the technical field of potato breeding, in particular to a breeding method of resistant starch and anthocyanin polymerized potatoes.
Background
The content of the existing grain safety is changed from the pure grain quantity safety into comprehensive safety combining quantity, economy, quality, ecology and the like. At present, the economic benefit of common varieties of crops is relatively low, so that the traditional 'grain crops' is improved into 'grain economic compromise type crops', and the method has important significance for improving the added value of the crops and improving the healthy development of agriculture. The functional potato is the key for promoting the potential of staple food consumption and the charm of food innovation.
Anthocyanins are one of the bioflavonoids and are the most effective natural antioxidants currently recognized for scavenging free radicals in the human body. Colored potatoes are rich in anthocyanin and become another hotspot of potato breeding. According to the estimation of healthy Chinese actions, the prevalence rate of diabetes in China is 12.8 percent, and the number of patients is 1.5 hundred million; the prevalence rate of dyslipidemia is 18.6%, and patients are 1.6 hundred million. Resistant Starch (RS) is a general name of starch and starch degradation products which are difficult to digest and absorb in the small intestine of a healthy human body, and has multiple important physiological functions of reducing blood sugar, reducing blood fat, controlling weight, being beneficial to intestinal health and the like. In general, the resistant starch (RS 2) is higher in raw potatoes but lower in cooked resistant starch (RS 3), and both raw and potato resistant starches are high for "double high" potato use.
Therefore, the breeding method of the resistant starch and anthocyanin polymerized potato is provided, the new potato functional variety with double high resistant starch and high anthocyanin is bred, the added value and the innovative functional food are improved, the nutritional requirements of healthy Chinese actions are met, and the quality improvement and the efficiency improvement are promoted to boost the country happiness.
Disclosure of Invention
The invention provides a breeding method of resistant starch and anthocyanin polymerized potatoes, and the new potato variety with the function characteristics obtained by the breeding method is beneficial to improving the additional value and developing functional foods.
The specific technical scheme is as follows:
the invention provides a breeding method of resistant starch and anthocyanin polymerized potatoes, which comprises the following steps:
(1) Hybridizing the purple skin purple flesh potato serving as a male parent and the purple skin yellow flesh potato serving as a female parent to obtain F 1 Seed generation;
(2) F is to be 1 Seedling raising and planting the seed generation seeds in a greenhouse, harvesting the small potatoes, reserving tubers of purple potato seed coats in the small potatoes, cutting the tubers into two pieces, taking one piece of purple potato pulp in the tubers, measuring resistant starch RS2 in the raw potatoes, measuring resistant starch RS3 in the cooked potatoes, selecting the small potatoes corresponding to samples with RS2 being more than or equal to parent A and RS3 being more than or equal to parent B, and producing to obtain the small potatoes with S 1 ；
(3) Planting S 1 Harvesting potato blocks, and observing the seed coat and the meatHomozygous stable purple skin and purple meat strain, determining RS2 and RS3 content, selecting clone S with RS2 more than or equal to parent A and RS3 more than or equal to parent B 2 ；
(4) Planting S 2 Measuring S 2 The resistant starch of (1) is selected and retained with double high clone S with RS2 more than or equal to 45 percent and RS3 more than or equal to 5 percent 3 ；
(5) Planting S 3 Measuring S 3 Selecting clone S with reducing sugar content not more than that of parent 4 ；
(6) Planting S 4 Measuring S 4 Selecting a strain with the anthocyanin content of more than or equal to 4.0mg/g dw, and finally breeding to obtain the resistant starch and anthocyanin polymerized potato.
Further, the potatoes of the purple skin and purple meat are purple pauli. The purple treasure is a new color potato variety introduced in Canada, and related information is visible: ma Shi Rong, purple Black Potato breed-Zi Bao Li, rural Bai Shi Tong, 2011.
Further, the purple-skin yellow-fleshed potato is medium potato No. 3. The Zhongshu No. 3 is a new species of fresh-eating potato bred by vegetable and flower institute of Chinese academy of agricultural sciences, and the variety approval certificate No. is Guiju potato No. 2004003.
See the reducing sugar content determination methods described above: measuring reducing sugar in GB/T5009.7-2003 food; see the methods for anthocyanin content determination described above: determination of anthocyanin in GB/T22244-2008 health food.
Compared with the prior art, the invention has the following beneficial effects:
(1) The breeding method provided by the invention has the advantages that the breeding method can realize fresh eating and puffing, is expected to develop more abundant functional leisure food, and can improve the quality and the efficiency of future villages.
(2) According to the invention, parents of purple seed coats and purple sweet potato pulp are used as female parents, and the genetic characteristics of purple characters are utilized, so that the expected breeding of high anthocyanin can be better realized, the purple sweet potato pulp is convenient and visual to identify and screen, the number of samples for determining two types of resistant starch through complicated processes is obviously reduced, the breeding technology is strong in pertinence, and the advantages of labor saving and cost saving are achieved.
(3) The anthocyanin of the bred resistant starch and anthocyanin polymerized potato is obviously higher than that of the parents, the fat content is improved under the background of high-resistance starch, and the prerequisite condition for the accumulation of fat-soluble anthocyanin is improved.
Detailed Description
The present invention will be further described with reference to the following specific examples, which are set forth merely to illustrate specific embodiments of the invention, but the scope of the invention is not limited thereto.
The breeding method provided in the following examples is a breeding method of resistant starch and anthocyanin-polymerized potatoes based on medium potato No. 3 with higher RS3 and purple sweet with higher RS 2.
Wherein the Zhongshu No. 3 is a new potato variety bred by vegetable and flower institute of Chinese academy of agricultural sciences, and the variety approval certificate No. is Guijushu No. 2004003. The parent RS3 content is 4.5%, the RS2 content is 30.6%, and the anthocyanin content is 0.3mg/g dw, and the parent can be purchased from Chinese agricultural academy of sciences or general markets.
The purple sweet potato is a new potato variety with purple skin and purple meat introduced in Canada, and the related information is visible: ma Shi Rong, purple Black Potato breed-Zi Bao Li, rural Bai Shi Tong, 2011. The parent anthocyanin contains 4.5mg/g dw, 3.6% of RS3 and 41.5% of RS2, and can be purchased from a common market.
Example 1 genetic expression Studies on purple Potato Peel and purple Potato pulp
The research on the hybridized population of purple skin purple meat, potato No. 3 in yellow skin yellow meat and purple skin yellow meat and potato No. 9 shows that the purple potato meat and the purple skin have obvious correlation, and the purple seed skin of the common potato meat is purple, which provides a theoretical basis for the intuitive selection of the purple skin, although the purple skin potato is not necessarily the purple meat; purple flesh is used as a female parent, and the purple flesh selection rate of filial generations is obviously higher than that of a male parent, so that guidance is provided for selecting parents of the invention.
Example 2
A breeding method of resistant starch and anthocyanin polymerization potatoes comprises the following specific steps:
(1) Zibaoli as male parentAnd the 3 # of the mesopotato is taken as a female parent to carry out hybridization to obtain F 1 Seed generation;
(2)F 1 planting seed generation in greenhouse, harvesting small potato, reserving purple seed coat tuber, cutting into two pieces, selecting one piece of purple potato pulp, measuring RS2, then measuring RS3, selecting corresponding small potato with RS2 not less than 41.5% and RS3 not less than 4.5%, and producing to obtain S 1 23 are used;
(3) Planting S 1 Harvesting potato blocks, observing the homozygous and stable plant line with purple peel and purple flesh of the seed peel and potato flesh, measuring the RS2 and RS3 contents, and selecting and reserving clone S with the RS2 content being more than or equal to 41.5 percent and the RS3 content being more than or equal to 4.5 percent 2 11, the number of the channels is 11;
(4) Planting S 2 Comprehensively evaluating the resistant starch, selecting and reserving clone S with RS2 more than or equal to 45 percent and RS3 more than or equal to 5 percent 3 9, the number of the channels is 9;
(5) Planting S 3 Determining the content of reducing sugar, selecting clone S with the content of reducing sugar less than or equal to that of parents 4 3, the number of the cells is 3;
(6) Planting S 4 Evaluating the anthocyanin content, selecting and reserving a strain with the anthocyanin more than or equal to 4.0mg/g dw, and finally breeding 1 of resistant starch and anthocyanin polymerized potatoes, wherein the potato is originally named as a flour-resistant purple potato.
A comparison of dry matter, reducing sugars, RS2, RS3, fat, estimated glycemic index, and anthocyanins for each variety is shown in Table 2.
In Table 2, the comparison of various character indexes of the 'flour-resistant purple sweet potato' bred by the hybridization of the No. 3 sweet potato and the Zibaoli
Remarking: * Representing a very significant difference at the 0.05 level
The measurement method of each index in table 2 is as follows: dry matter: GBT8858-1988 method for measuring dry substance and water content in fruit and vegetable products; the method for measuring the content of reducing sugar comprises the following steps: measuring reducing sugar in GB/T5009.7-2003 food; RS2 and RS3: the flues of chain length of amyloid on nutrient Starch in rice (Oryza sativa L.). Starch-
2007,59; fat: measuring fat in GB/T5009.6-2003 food; estimate glycemic index (eGI):
a stand hydrolytics procedure to estimate glycemic index, nutrition Research,1997, 17; anthocyanins: GB/T22244-2008.
Through a grade ratio test, the bred flour-resistant purple sweet potatoes have dry matter content of 18.9%, reducing sugar content of 0.29%, RS2 content of 45.3%, RS3 content of 5.1%, fat content of 0.5%, estimated blood glucose generation index of only 57.3 and anthocyanin content of 13.6mg/kg. Wherein, the content of reducing sugar is lower than that of the parents, and the content of RS2 and RS3 is higher than that of the parents, so the potato micro-cultivation medium is particularly suitable for people with diabetes and hyperlipidemia, which is related to the high dry matter content of the variety and the possible somatic variation in the process of cultivating the micro-potatoes.
The estimated glycemic index is significantly lower, which is a result of the combined effect of low reducing sugar content, high RS2 and high RS3, and also has a special reason of high fat content, because fat easily forms amylose-lipid complex with amylose, which plays a positive role in effectively preventing amylase from being digested by contact.
The anthocyanin of the bred nutrient compound potato is obviously higher than that of the parents, the super parents appear, and except for the reason of high dry matter, the anthocyanin is a fat-soluble chemical substance, and the high fat content improves the prerequisite condition for the accumulation of the more abundant anthocyanin in the potato pulp.
Therefore, the bred variety has the characteristic of better function in the aspect of nutrient composition, is required by healthy Chinese actions, is suitable for people with special diabetes and hyperlipidemia, and has stronger antioxidant function. In addition, the bred variety has high RS2 and RS3, and is both fresh food and puffing, so that the method is expected to develop richer functional leisure food and is helpful for quality improvement and efficiency improvement of future villages.
Claims (1)
1. A breeding method of resistant starch and anthocyanin polymerized potatoes is characterized by comprising the following steps:
(1) Hybridizing the purple-Baoli as male parent and the yam No. 3 as female parent to obtain F 1 Seed generation;
（2）F 1 planting seed generation in greenhouse, harvesting small potato, reserving purple seed coat tuber, cutting into two pieces, selecting one piece of purple potato pulp, measuring RS2, then measuring RS3, selecting corresponding small potato with RS2 not less than 41.5% and RS3 not less than 4.5%, and producing to obtain S 1 ；
(3) Planting S 1 Harvesting potato blocks, observing the homozygous and stable plant line with purple peel and purple flesh of the seed peel and potato flesh, measuring the RS2 and RS3 contents, and selecting and reserving clone S with the RS2 content being more than or equal to 41.5 percent and the RS3 content being more than or equal to 4.5 percent 2 ；
(4) Planting S 2 Comprehensively evaluating the resistant starch, selecting and reserving clone S with RS2 more than or equal to 45 percent and RS3 more than or equal to 5 percent 3 ；
(5) Planting S 3 Determining the content of reducing sugar, selecting clone S with the content of reducing sugar less than or equal to that of parent 4 ；
(6) Planting S 4 Evaluating the anthocyanin content, selecting and reserving a strain with the anthocyanin more than or equal to 4.0mg/g dw, and finally breeding the resistant starch and anthocyanin polymerized potatoes.
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| CN116724884A (en) * | 2023-07-27 | 2023-09-12 | 上海浙江大学高等研究院 | Breeding method of double-harvest potatoes with solanaceous fruit and potato blocks |
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| CN107155869A (en) * | 2017-05-27 | 2017-09-15 | 西北农林科技大学 | A kind of breeding method rich in anthocyanidin black potato |
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| WO2021173944A1 (en) * | 2020-02-26 | 2021-09-02 | Lodaat, Llc | Highly resistant starch and manufacturing process therefor |
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| CN107155869A (en) * | 2017-05-27 | 2017-09-15 | 西北农林科技大学 | A kind of breeding method rich in anthocyanidin black potato |
| CN107926698A (en) * | 2017-12-29 | 2018-04-20 | 云南省农业科学院经济作物研究所 | A kind of high pigment content color potato breed breeding method |
| WO2021173944A1 (en) * | 2020-02-26 | 2021-09-02 | Lodaat, Llc | Highly resistant starch and manufacturing process therefor |
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