CN114427404A - Microbial huff-puff oil production method for strong-edge-bottom water heavy oil reservoir - Google Patents
Microbial huff-puff oil production method for strong-edge-bottom water heavy oil reservoir Download PDFInfo
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- 239000003921 oil Substances 0.000 title claims abstract description 100
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 94
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 38
- 239000000295 fuel oil Substances 0.000 title claims abstract description 31
- 230000000813 microbial effect Effects 0.000 title claims abstract description 24
- 239000001913 cellulose Substances 0.000 claims abstract description 103
- 229920002678 cellulose Polymers 0.000 claims abstract description 103
- 238000012360 testing method Methods 0.000 claims abstract description 70
- 244000005700 microbiome Species 0.000 claims abstract description 62
- 150000004676 glycans Chemical class 0.000 claims abstract description 61
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 61
- 239000005017 polysaccharide Substances 0.000 claims abstract description 61
- 238000000034 method Methods 0.000 claims abstract description 45
- 230000000694 effects Effects 0.000 claims abstract description 37
- 230000008569 process Effects 0.000 claims abstract description 29
- 238000012216 screening Methods 0.000 claims abstract description 25
- 238000011084 recovery Methods 0.000 claims abstract description 21
- 238000010276 construction Methods 0.000 claims abstract description 17
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 12
- 239000003129 oil well Substances 0.000 claims abstract description 12
- 230000003213 activating effect Effects 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 69
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 63
- 235000015097 nutrients Nutrition 0.000 claims description 44
- 239000000243 solution Substances 0.000 claims description 43
- 239000007924 injection Substances 0.000 claims description 41
- 238000002347 injection Methods 0.000 claims description 41
- 241000894006 Bacteria Species 0.000 claims description 30
- 238000000855 fermentation Methods 0.000 claims description 26
- 230000004151 fermentation Effects 0.000 claims description 26
- 241000192023 Sarcina Species 0.000 claims description 24
- 239000012190 activator Substances 0.000 claims description 19
- 238000002360 preparation method Methods 0.000 claims description 17
- 241000589158 Agrobacterium Species 0.000 claims description 16
- 238000006073 displacement reaction Methods 0.000 claims description 15
- 239000012530 fluid Substances 0.000 claims description 14
- 230000001580 bacterial effect Effects 0.000 claims description 13
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 13
- 239000000843 powder Substances 0.000 claims description 13
- 230000004060 metabolic process Effects 0.000 claims description 11
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 10
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 9
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 8
- 230000008859 change Effects 0.000 claims description 8
- 238000011156 evaluation Methods 0.000 claims description 8
- 241000589155 Agrobacterium tumefaciens Species 0.000 claims description 7
- 241000237858 Gastropoda Species 0.000 claims description 6
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims description 5
- 229930091371 Fructose Natural products 0.000 claims description 5
- 239000005715 Fructose Substances 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000004202 carbamide Substances 0.000 claims description 5
- 239000010779 crude oil Substances 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 5
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 5
- 235000013379 molasses Nutrition 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 4
- 230000000638 stimulation Effects 0.000 claims description 4
- 244000235858 Acetobacter xylinum Species 0.000 claims description 3
- 235000002837 Acetobacter xylinum Nutrition 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims description 2
- 230000006698 induction Effects 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 2
- BJHIKXHVCXFQLS-UYFOZJQFSA-N fructose group Chemical group OCC(=O)[C@@H](O)[C@H](O)[C@H](O)CO BJHIKXHVCXFQLS-UYFOZJQFSA-N 0.000 claims 1
- 238000011161 development Methods 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 8
- 235000019797 dipotassium phosphate Nutrition 0.000 description 5
- 230000009545 invasion Effects 0.000 description 5
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 4
- 239000010881 fly ash Substances 0.000 description 4
- 239000008398 formation water Substances 0.000 description 4
- 230000035699 permeability Effects 0.000 description 4
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 3
- 235000011941 Tilia x europaea Nutrition 0.000 description 3
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- 239000004571 lime Substances 0.000 description 3
- 238000006241 metabolic reaction Methods 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 3
- 235000019353 potassium silicate Nutrition 0.000 description 3
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 description 3
- 230000033558 biomineral tissue development Effects 0.000 description 2
- 239000004927 clay Substances 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 238000009991 scouring Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- KXGFMDJXCMQABM-UHFFFAOYSA-N 2-methoxy-6-methylphenol Chemical compound [CH]OC1=CC=CC([CH])=C1O KXGFMDJXCMQABM-UHFFFAOYSA-N 0.000 description 1
- 241000626621 Geobacillus Species 0.000 description 1
- 238000010793 Steam injection (oil industry) Methods 0.000 description 1
- 241001106462 Ulmus Species 0.000 description 1
- 239000002981 blocking agent Substances 0.000 description 1
- 230000005465 channeling Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012760 heat stabilizer Substances 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 229920001568 phenolic resin Polymers 0.000 description 1
- 239000005011 phenolic resin Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000012744 reinforcing agent Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- RMAQACBXLXPBSY-UHFFFAOYSA-N silicic acid Chemical compound O[Si](O)(O)O RMAQACBXLXPBSY-UHFFFAOYSA-N 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 229920001187 thermosetting polymer Polymers 0.000 description 1
Classifications
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/582—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of bacteria
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
- E21B43/24—Enhanced recovery methods for obtaining hydrocarbons using heat, e.g. steam injection
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- Engineering & Computer Science (AREA)
- Mining & Mineral Resources (AREA)
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- General Life Sciences & Earth Sciences (AREA)
- Fluid Mechanics (AREA)
- Physics & Mathematics (AREA)
- Environmental & Geological Engineering (AREA)
- Geochemistry & Mineralogy (AREA)
- Organic Chemistry (AREA)
- Materials Engineering (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a microbial huff and puff oil production method for a heavy oil reservoir with strong edge water and bottom water, which comprises the following steps of: (1) screening a test oil reservoir; (2) screening the cellulose polysaccharide-producing microorganisms and determining the most appropriate concentration of the cellulose polysaccharide-producing microorganisms; (3) screening out a proper formula of the activating agent; (4) carrying out field test; (5) and opening the well for production; (6) and evaluating the effect. Compared with the prior art, the invention has the following advantages and beneficial effects: (1) the invention has wide range, is suitable for a plurality of strong edge bottom water thickened oil wells and also suitable for strong edge bottom water thickened oil wells with water plugging failure or ineffectiveness; (2) the thick oil reservoir development effect can be effectively improved; (3) the field test effect is good, and the oil increase of a single well is 300-500 tons in the field test; (4) the construction process is simple, the operation is simple and easy, the operability is strong, the investment cost is low, the field test effect is good, the input-output ratio is more than 1:3.8, and the recovery ratio is improved by more than 10%.
Description
Technical Field
The invention belongs to the technical field of microbial oil recovery, and relates to a microbial huff-puff oil recovery method for a heavy oil reservoir with strong edge bottom water.
Background
The heavy oil resources occupy a large proportion of the petroleum reserves, but most heavy oil reservoirs have active bottom water. In the long-term pressure reduction exploitation process of steam injection throughput, the pressure of an oil reservoir is reduced to cause the edge bottom water to advance, the water content of a water flooded well zone is increased rapidly, the water invasion range is enlarged continuously, and the control reserve of the water invaded zone accounts for about 70% of the total consumed reserve sometimes.
In order to improve the final recovery ratio of the side-bottom water heavy oil reservoir, a water plugging process of an oil well needs to be implemented to form a plugging partition plate with a large area, the injected plugging agent has a filling and compacting effect on a formed bottom water channeling channel and a high permeable layer, and the plugging rate and the plugging strength are high, so that the plugging agent can bear the scouring of steam condensate and formation water.
Three types of water plugging agents are usually selected, one is a high-temperature profile control agent which mainly comprises polyacrylamide, an organic cross-linking agent, high-temperature-resistant oil-soluble resin, rubber powder, elm bark powder, an inorganic reinforcing agent and a heat stabilizer and has better temperature resistance and steam scouring resistance strength; the water glass clay plugging agent mainly comprises water glass, clay, lime and formamide (or formaldehyde), wherein the water glass and the formamide form alkaline silicic acid gel with a net structure; and the third is a high-strength plugging agent of resin fly ash, which is used for sealing and mainly comprises thermosetting powder phenolic resin, fly ash, cement, soil and lime. The fly ash of coal-fired thermal power plants has good temperature resistance, stable chemical property, easy separation, low price and wide source. The fly ash is compounded with cement, soil and lime to produce similar hardening reaction with cement to form high strength blocking agent.
The method is applied to control the invasion of the bottom water to a certain extent, but when the method is implemented on site, because the plugging agent solutions have certain viscosity, the injection capability is poor, the permeability of the stratum is also damaged to a certain extent, the strength of some plugging agents is insufficient, or the temperature resistance is insufficient, and the plugging agent is easy to lose efficacy in the steam huff and puff at the later stage, so that the plugging effect is poor, and the effective period is short.
Disclosure of Invention
The purpose of the invention is as follows: aiming at the defects in the prior art, the invention provides a microbial huff and puff oil production method for a heavy oil reservoir with strong edge bottom water, which has the characteristics of strong pertinence, wide application range, strong operability, low cost and obvious oil increasing effect.
The technical scheme is as follows: a microbial huff and puff oil production method for a strong edge bottom water heavy oil reservoir comprises the following steps:
(1) screening a test oil reservoir;
(2) screening the cellulose polysaccharide-producing microorganisms and determining the most appropriate concentration of the cellulose polysaccharide-producing microorganisms;
(3) screening out a proper formula of the activating agent;
(4) carrying out field test;
(5) and opening the well for production;
(6) and evaluating the effect.
Further, the screening criteria of the test oil reservoir in the step (1) are as follows:
selecting oil well with strong edge bottom water and thick oil, oil deposit temperature<50 ℃, the viscosity of crude oil is between 10000mPa.s and 30000mPa.s, and the liquid amount is 30-50m3And the water content of the produced liquid reaches 90-100%.
Further, the step (2) comprises the following steps:
(21) taking 150ml of output liquid of the tested oil reservoir screened in the step (1), filling the output liquid into anaerobic bottles, respectively performing aseptic operation to access a plurality of cellulose polysaccharide-producing microorganisms and nutrient solution thereof, then placing the anaerobic bottles in a constant temperature box to be cultured for 15-30d, setting the temperature of the constant temperature box as the oil reservoir temperature of the tested oil reservoir, observing the cellulose metabolism condition in the experimental process, measuring the dry weight of cellulose polysaccharide, and screening the cellulose polysaccharide-producing microorganisms with the strongest cellulose polysaccharide-producing capability;
(22) and (2) filling 150ml of the output liquid of the test oil reservoir screened in the step (1) into an anaerobic bottle, carrying out aseptic operation on the cellulose-producing polysaccharide microorganism screened in the step (21) with the concentration of 2-10% and the nutrient solution thereof, then placing the anaerobic bottle in a constant temperature box for culturing for 15-30d, setting the temperature of the constant temperature box as the oil reservoir temperature of the test oil reservoir, observing the cellulose metabolism condition in the experimental process, measuring the dry weight of the cellulose polysaccharide, and determining the optimal concentration of the cellulose-producing polysaccharide microorganism.
Further, the volume of the anaerobic bottle in the steps (21) and (22) is 200 mL.
Still further, the cellulose-producing polysaccharide microorganisms include Acetobacter xylinum, Agrobacterium, and Sarcina.
More particularly, the formula of the nutrient solution of the acetic acid bacteria comprises 2 to 5 percent of glucose, 0.2 to 0.5 percent of yeast powder, 0.1 to 0.3 percent of dipotassium phosphate and MgSO 44·7H20.1 to 0.3 percent of O and the balance of water.
More particularly, the nutrient solution formula of the agrobacterium is 2-4% of fructose, 0.1-0.3% of urea, 0.1-0.2% of yeast powder and K2HPO4 0.1-0.4％、FeSO4·7H20.01 to 0.02 percent of O and the balance of water.
More particularly, the nutrient solution formula of sarcina comprises 3-5% of molasses, 0.2-0.8% of ammonium nitrate, 0.2-0.4% of dipotassium phosphate, 0.1-0.3% of potassium dihydrogen phosphate, 0.03-0.1% of sodium chloride and the balance of water.
Further, the step (3) comprises the following steps:
and (2) filling 150ml of produced liquid of the tested oil reservoir screened in the step (1) into an anaerobic bottle, and configuring the C/N ratio to be (3-25): 1, inoculating the nutrient solution of the cellulose-producing polysaccharide microorganism screened in the step (2) into the cellulose-producing polysaccharide microorganism fermentation liquor with the optimal concentration determined in the step (2) through aseptic operation, placing an anaerobic bottle in a constant temperature box to culture for 15-30 days at the oil reservoir temperature, observing the bacterial concentration condition in the culture process, determining the cellulose yield, and further determining the optimal C/N ratio.
Further, the volume of the anaerobic bottle in the step (3) was 200 mL.
Further, the step (4) comprises the following steps:
(41) before the construction of injecting cellulose polysaccharide-producing microorganism, 20m is used3Washing the well with the hot water;
(42) cellulose-producing polysaccharide bacterial liquid and injection of nutrient solution thereof
(421) Respectively configuring a proper activator formula (nutrient solution) determined in the step (3) and the cellulose-producing polysaccharide microorganism fermentation liquor with the optimal concentration determined in the step (2) at a wellhead of a test oil reservoir, then forming a first slug and a second slug by the configured proper activator formula (nutrient solution) determined in the step (3) and the cellulose-producing polysaccharide microorganism fermentation liquor with the optimal concentration according to the volume ratio of 3:1 and 3:2, and quickly injecting the slugs into the wellhead of the test oil reservoir, wherein:
the first displacement of the slug is 20m3Over h, construction pressure controlled at 12-20 MPa, total injection amount 400m3～800m3；
The second displacement of the slug is 15m3Over h, construction pressure controlled at 12-20 MPa, total injection amount 400m3～800m3；
(422) Injecting 15-40 m into the wellhead of the test oil reservoir3Using clear water as displacement liquid, and closing the well;
(433) and (5) observing the pressure change of the well mouth after closing the well, and entering the step (5) when the pressure does not rise any more and is kept for 5 days continuously.
Further, the suitable activator formulation in step (421) is formulated at the wellhead with injection water, and the cellulose polysaccharide-producing microorganism fermentation broth of suitable concentration is transported to the wellhead by tanker truck.
Further, in step (421), two preparation pools are prepared at the wellhead of the test reservoir, one preparation pool is used for preparing the well fluid, and the other preparation pool is used for injecting the well fluid into the wellhead.
Still further, the volumes of the two liquid preparation pools in the step (421) are both 20m3。
Further, if the amount of the produced liquid is low, the production liquid is less than 10m in the step (5)3One steam stimulation or steam induction cycle can be performed.
Further, the indexes of the field test effect evaluation in the step (6) comprise oil increment, effective period and input-output ratio.
The invention uses microorganism to synthesize cellulose polysaccharide in oil deposit, which can effectively realize the plugging of bottom water:
firstly, the working fluid is prepared by injected water, the main components are cellulose-producing polysaccharide microbial solution and an activator nutrient system, the viscosity of the formed aqueous solution is lower than 5mPa.s, the formed aqueous solution has good injection capability, and the aqueous solution can directly enter a water channel formed by water invasion after injection;
secondly, the polysaccharide synthesized by the cellulose-producing polysaccharide microorganism in the oil reservoir water channel porous medium is cellulose polysaccharide, is water-insoluble, has higher strength and stability, and has almost unchanged sealing and mining capacity even encountering high-temperature steam.
Therefore, the invention can effectively improve the problem of high water content of the oil well caused by edge bottom water after water-drive thickened oil development and steam thermal recovery, improve the yield of the thickened oil well, prolong the production period of the oil well and improve the recovery ratio of the thickened oil.
Has the advantages that: compared with the prior art, the invention has the following advantages and beneficial effects:
(1) the invention has wide application range, is suitable for a plurality of strong edge bottom water thick oil wells and also suitable for strong edge bottom water thick oil wells with water plugging failure or ineffectiveness;
(2) cellulose is synthesized in the oil reservoir by microorganisms, so that the invasion of edge bottom water is controlled, and the development effect of the heavy oil reservoir can be effectively improved;
(3) the strong edge bottom water heavy oil reservoir microorganism huff and puff technology formed by the invention has the function of synthesizing cellulose polysaccharide, can directly enter a channel formed by water invasion after injection, synthesizes high-strength water-insoluble cellulose polysaccharide under the oil reservoir condition, has stronger plugging performance, is stable and high temperature resistant, can effectively improve the problem of high water content of an oil well caused by edge bottom water after water-drive heavy oil development and steam thermal recovery, improves the yield of a heavy oil well, prolongs the production period of the oil well and improves the recovery ratio of the heavy oil. The comprehensive effect leads to good field test effect, and the oil increase of a single well is 300-500 tons in the field test;
(4) the invention has the advantages of simple construction process, simple operation, strong operability, low investment cost and good field test effect, the input-output ratio is more than 1:3.8, and the recovery ratio is improved by more than 10%.
The specific implementation mode is as follows:
the present invention will be further described with reference to specific examples.
The percentages in this document are, unless otherwise specified, percentages by mass.
Example 1
Microbial huff-puff oil production method for strong-edge-bottom water heavy oil reservoir
(1) Screening of test oil reservoirs:
summary of experimental single well a of a certain oil production plant in the victory oil field: oil reservoir temperature is 28 ℃, oil layer thickness is 4.5m, and permeability is 850 multiplied by 10-3μm2The mineralization degree of formation water is 26458mg/L, the viscosity of crude oil is 11560 mPa.s, the water content is 99 percent, and the liquid amount is 50m3Geological reserve of 7.5 x 104And t, the test single well A meets the screening standard of the test oil reservoir, so that the test single well A is used as the test oil reservoir.
(2) Screening and determining the appropriate concentration of cellulose-producing polysaccharide microorganisms:
(21) 150mL of output liquid of a test single well A is filled into an anaerobic bottle with the volume of 200mL, various cellulose polysaccharide-producing microorganisms (acetic acid bacteria, agrobacterium tumefaciens and sarcina) and nutrient solution thereof are inoculated into the anaerobic bottle in an aseptic operation, then the anaerobic bottle is placed in an incubator to be cultured for 15d, the temperature of the incubator is set to be 28 ℃, the cellulose metabolism condition is observed in the experimental process, the dry weight of the cellulose polysaccharide is measured, and the specific data are shown in Table 1 to screen out the microorganisms with the strongest cellulose polysaccharide-producing capability.
The formula of the nutrient solution of the acetic acid bacteria comprises 3 percent of glucose, 0.4 percent of yeast powder, 0.2 percent of dipotassium hydrogen phosphate and MgSO4·7H20.2% of O and the balance of water;
the nutrient solution of Agrobacterium comprises fructose 3%, urea 0.2%, yeast powder 0.15%, and K2HPO4 0.3％、FeSO4·7H20.01 percent of O and the balance of water;
the nutrient solution formula of sarcina comprises 4 percent of molasses, 0.5 percent of ammonium nitrate, 0.3 percent of dipotassium phosphate, 0.2 percent of monopotassium phosphate, 0.05 percent of sodium chloride and the balance of water;
as can be seen from Table 1, the concentration of Acetobacter xylinum is 8.5X 10 at the maximum9The yield of the cellulose reaches 2g/L at most.
TABLE 1 Effect of cellulose production by different functional microorganisms
| Cellulose microorganism | Concentration of bacteria (10)8one/mL) | Dry weight g of cellulose |
| Acetic acid bacteria | 85 | 2 |
| Agrobacterium tumefaciens | 15 | 0.8 |
| Sarcina | 40 | 1.1 |
(22) And (3) taking 150mL of output liquid of the single well A to be tested, filling the output liquid into a 200mL anaerobic bottle, aseptically operating acetic acid bacteria fermentation liquid with the concentration of 2-10% and nutrient solution thereof, then placing the anaerobic bottle in a constant temperature box to culture for 15d, setting the temperature of the constant temperature box to be 28 ℃, observing the cellulose metabolism condition in the experimental process, measuring the dry weight of cellulose polysaccharide, and determining the most appropriate concentration of the cellulose polysaccharide-producing microorganisms. As is clear from Table 2, the effect of the acetic acid bacteria fermentation broth having a concentration of 10% was the best.
TABLE 2 effect of yield of acetic acid bacteria fermentation broth of different concentrations
| Cellulose microorganism | Concentration of bacteria (10)8one/mL) | Dry weight g/L of cellulose |
| 2％ | 70 | 1.4 |
| 4％ | 72 | 1.5 |
| 6％ | 76 | 1.8 |
| 8％ | 84 | 2 |
| 10％ | 96 | 2.2 |
(3) According to the growth requirement of acetic acid bacteria, screening out a proper activator formula (nutrition system):
150mL of output liquid of the test single well A is taken and filled into an anaerobic bottle with the volume of 200mL, nutrient solutions (3: 1; 10: 1; 20: 1; 25: 1) with different C/N ratios are prepared, acetic acid bacteria fermentation liquor with the concentration of 10% is inoculated into the anaerobic bottle through aseptic technique, and then the anaerobic bottle is placed into an incubator to be cultured for 15d, and the temperature of the incubator is set to be 28 ℃. And observing the bacterial concentration of the acetic acid bacteria in the culture process, and determining the cellulose yield. As can be seen from Table 3, the best cellulose metabolism by acetic acid bacteria was selected when the C/N ratio of the nutrient system was 10: 1.
TABLE 3 cellulose metabolizing Effect of different C/N ratios of acetic acid bacteria
| C/N ratio of nutrient solution | Concentration of bacteria (10)8one/mL) | Dry weight g of cellulose |
| 3:1 | 20 | 0.2 |
| 10:1 | 90 | 2.1 |
| 20:1 | 85 | 2.0 |
| 25:1 | 80 | 1.8 |
(4) The microorganism huff and puff injection process is formed according to the characteristic of high water content of a test single well A, and is mainly divided into two slugs for injection, and the specific process is as follows:
(41) before the microorganism injection construction, 20m is used3Washing the well with the hot water;
(42) two prepared 20m at wellhead3The liquid preparation pool is used for preparing the working fluid, the liquid preparation pool is used for injecting the working fluid into the test single well A, bacterial liquid (10% acetic acid bacteria fermentation liquid) is transported to a well head by a tank truck, an activator formula with the CN ratio of 10:1 is prepared at the well head of the test single well A by using injection water, after the preparation, the bacterial liquid and the activator formula form a slug I and a slug II according to the volume ratios of 3:1 and 3:2 respectively, and the slugs are quickly injected;
(43) the first displacement of the slug is 20m3H, the construction pressure is controlled to be 20MPa, and the total injection amount is 400m3；
The second displacement of the slug is 15m3H, the construction pressure is controlled to be 20MPa, and the total injection amount is 400m3；
Final injection of 30m3Clear water is used as displacement liquid, and the injection speed is 15m3And h, closing the well and observing.
(44) And (3) observing the pressure change of the well mouth after closing the well, opening the well for production after the pressure does not rise any more and is kept for 5 days continuously, wherein the well closing time is 20 days, so that microorganisms are fully fermented and subjected to metabolic reaction underground, and the sleeve pressure and oil pressure change is observed during the well closing period.
(5) And (3) well opening production:
after the well is opened, the production dynamic is monitored, the water content is reduced to 58 percent,liquid amount of 15m310 tons of daily oil;
if the well-opening production liquid amount is low, the well-opening production liquid amount is less than 10m3One round of steam huff and puff or steam effect introduction can be carried out;
(6) evaluation of the effects:
performing field test according to the injection process determined in the above steps, wherein the injection amount of 10% acetic acid bacteria fermentation liquor and activator in volume ratio of 3:1 and 3:2 is 800m3. And after the field test is finished, evaluating the effect of the field test, wherein the evaluation indexes comprise the oil increment, the validity period and the input-output ratio.
After field implementation, the single well A increases oil by 860 tons cumulatively, the validity period is 80 days, the input-output ratio is 1:3.8, and the field test effect is good.
Example 2
Microbial huff-puff oil production method for strong-edge-bottom water heavy oil reservoir
(1) Screening of test oil reservoirs:
summary of experimental single well B of certain oil production plant in victory oil field: oil reservoir temperature is 35 ℃, oil layer thickness is 6m, and permeability is 952 multiplied by 10-3μm2The salinity of formation water is 23458mg/L, the viscosity of crude oil is 14560mPa & s, the water content is 91 percent, and the liquid amount is 40m3Geological reserve of 8.5 x 104And t, the test single well B meets the screening standard of the test oil reservoir, so that the test single well B is used as the test oil reservoir.
(2) Screening and determining the appropriate concentration of cellulose-producing polysaccharide microorganisms:
(21) 150mL of output liquid of the test single well B is filled into an anaerobic bottle with the volume of 200mL, various cellulose polysaccharide-producing microorganisms (acetic acid bacteria, agrobacterium tumefaciens and sarcina) and nutrient solution thereof are inoculated into the anaerobic bottle by aseptic operation, then the anaerobic bottle is placed in an incubator to be cultured for 30d, the temperature of the incubator is set to 35 ℃, the cellulose metabolism condition is observed in the experimental process, the dry weight of the cellulose polysaccharide is measured, and the specific data are shown in Table 4 to screen out the microorganisms with the strongest cellulose polysaccharide-producing capability.
The formula of the nutrient solution of the acetic acid bacteria comprises 2 percent of glucose, 0.5 percent of yeast powder, 0.1 percent of dipotassium hydrogen phosphate and MgSO4·7H20.3 percent of O and the balance of water;
the nutrient solution of Agrobacterium comprises fructose 4%, urea 0.1%, yeast powder 0.2%, and K2HPO40.1％、FeSO4·7H20.02% of O and the balance of water;
the nutrient solution formula of sarcina comprises 3 percent of molasses, 0.8 percent of ammonium nitrate, 0.2 percent of dipotassium phosphate, 0.3 percent of monopotassium phosphate, 0.03 percent of sodium chloride and the balance of water;
as can be seen from Table 4, the concentration of Sarcina was 5X 10 at the highest9The yield of the cellulose reaches 1.3g/L at most.
TABLE 4 cellulose yield effect of different functional microorganisms
| Cellulose microorganism | Concentration of bacteria (10)8one/mL) | Dry weight g/L of cellulose |
| Acetic acid bacteria | 35 | 1 |
| Agrobacterium tumefaciens | 25 | 0.9 |
| Sarcina | 50 | 1.3 |
(22) And (3) taking 150mL of output liquid of the single well B to be tested, filling the output liquid into a 200mL anaerobic bottle, aseptically operating the sarcina fermentation liquid with the concentration of 2-10% and nutrient solution thereof, then placing the anaerobic bottle in an incubator to culture for 30d, setting the temperature of the incubator to be 35 ℃, observing the cellulose metabolism condition in the experimental process, measuring the dry weight of cellulose polysaccharide, and determining the most appropriate concentration of the cellulose polysaccharide-producing microorganisms. As can be seen from Table 5, the fermentation broth of Sarcina at a concentration of 8% gave the best results.
TABLE 5 fermentation liquor yield effect of Sarcina varying concentrations
| Cellulose microorganism | Concentration of bacteria (10)8one/mL) | Dry weight g of cellulose |
| 2％ | 30 | 0.6 |
| 4％ | 40 | 0.8 |
| 6％ | 50 | 1.3 |
| 8％ | 75 | 1.8 |
| 10％ | 70 | 1.7 |
(3) According to the growth requirement of sarcina, screening out a proper activator formula (nutrition system):
150mL of output liquid of the test single well B is filled into an anaerobic bottle with the volume of 200mL, nutrient solutions (3: 1; 10: 1; 20: 1; 25: 1) with different C/N ratios are prepared, the sarcina fermentation liquid with the concentration of 8% is inoculated into the anaerobic bottle through aseptic operation, and then the anaerobic bottle is placed into an incubator to be cultured for 30 days, and the temperature of the incubator is set to be 35 ℃. And observing the bacterial concentration condition of sarcina in the culture process, and determining the cellulose yield. As can be seen from Table 6, sarcina were selected to be the best to metabolize cellulose when the C/N ratio of the nutrient system was 20: 1.
TABLE 6 cellulose metabolizing Effect of different C/N ratios of Sarcina on nutrient solutions
| C/N ratio of nutrient solution | Concentration of bacteria (10)8one/mL) | Dry weight g of cellulose |
| 3:1 | 20 | 0.3 |
| 10:1 | 45 | 2.1 |
| 20:1 | 90 | 2.3 |
| 25:1 | 88 | 1.8 |
(4) The microorganism huff and puff injection process is formed according to the characteristic of high water content of a test single well B, and is mainly divided into two slugs for injection, and the specific process is as follows:
(41) before the microorganism injection construction, 20m is used3Washing the well with the hot water;
(42) two prepared 20m at wellhead3The liquid preparation pool is used for preparing the well entering liquid, the liquid preparation pool is used for injecting the well entering liquid into the test single well B, the bacterial liquid (8% of sarcina fermentation liquid) is transported to a well head by a tank car, an activator formula with the CN ratio of 20:1 is prepared at the well head of the test single well B by using injection water, after the preparation, the bacterial liquid and the activator formula form a slug I and a slug II according to the volume ratios of 3:1 and 3:2 respectively, and the slug I and the slug II are injected quickly;
(43) the first displacement of the slug is 22m3H, the construction pressure is controlled to be 15MPa, and the total injection amount is 600m3；
The second displacement of the slug is 16m3H, the construction pressure is controlled to be 15MPa, and the total injection amount is 600m3；
Final injection of 15m3Clear water is used as displacement liquid, and the injection speed is 15m3And h, closing the well and observing.
(44) And (3) observing the pressure change of the well mouth after closing the well, opening the well for production after the pressure does not rise any more and is kept for 5 days continuously, wherein the well closing time is 20 days, so that microorganisms are fully fermented and subjected to metabolic reaction underground, and the sleeve pressure and oil pressure change is observed during the well closing period.
(5) And (3) well opening production:
after the well is opened, the production dynamics is monitored, the water content is reduced to 55 percent, and the liquid amount is 18m311 tons of daily oil;
if the well-opening production liquid amount is low, the well-opening production liquid amount is less than 10m3One round of steam huff and puff or steam effect introduction can be carried out;
(6) evaluation of the effects:
the injection process determined according to the steps is subjected to a field test, and the content of the injection process is 8 percentThe injection amount of sarcina fermentation liquor and the activator in a volume ratio of 3:1 to 3:2 is 1200m3. And after the field test is finished, evaluating the effect of the field test, wherein the evaluation indexes comprise the oil increment, the validity period and the input-output ratio.
After field implementation, 880 tons of oil are added into the single well B cumulatively, the validity period is 90 days, the input-output ratio is 1:4, and the field test effect is good.
Example 3
Microbial huff-puff oil production method for strong-edge-bottom water heavy oil reservoir
(1) Screening of test oil reservoirs:
summary of experimental individual well C for a certain oil production plant in the victory oil field: oil reservoir temperature is 20 ℃, oil layer thickness is 5.5m, and permeability is 750 multiplied by 10-3μm2The mineralization degree of the formation water is 22468mg/L, the viscosity of the crude oil is 12845mPa & s, the water content is 95 percent, and the liquid amount is 30m3Geological reserve of 9.2 x 104And t, the test single well C meets the screening standard of the test oil reservoir, so that the test single well C is used as the test oil reservoir.
(2) Screening and determining the appropriate concentration of cellulose-producing polysaccharide microorganisms:
(21) 150mL of output liquid of the test single well C is taken and filled into an anaerobic bottle with the volume of 200mL, various cellulose polysaccharide producing microorganisms (acetic acid bacteria, agrobacterium tumefaciens and sarcina) and nutrient solution thereof are inoculated in an aseptic operation, then the anaerobic bottle is placed in an incubator to be cultured for 25d, the temperature of the incubator is set to be 20 ℃, the cellulose metabolism condition is observed in the experimental process, the dry weight of the cellulose polysaccharide is measured, and the specific data are shown in Table 7 to screen out the microorganisms with the strongest cellulose polysaccharide producing capability.
The formula of the nutrient solution of the acetic acid bacteria comprises 5 percent of glucose, 0.2 percent of yeast powder, 0.3 percent of dipotassium hydrogen phosphate and MgSO4·7H20.1% of O and the balance of water;
the nutrient solution of Agrobacterium comprises fructose 2%, urea 0.3%, yeast powder 0.1%, and K2HPO40.4％、FeSO4·7H20.015 percent of O and the balance of water;
the nutrient solution formula of sarcina comprises 5 percent of molasses, 0.2 percent of ammonium nitrate, 0.4 percent of dipotassium phosphate, 0.1 percent of monopotassium phosphate, 0.1 percent of sodium chloride and the balance of water;
as can be seen from Table 7, the Agrobacterium concentration was up to 6.5X 109The yield of the cellulose reaches 1.5g/L at most.
TABLE 7 cellulose production Effect of different functional microorganisms
| Cellulose microorganism | Concentration of bacteria (10)8one/mL) | Dry weight g of cellulose |
| Acetic acid bacteria | 55 | 1.3 |
| Agrobacterium tumefaciens | 65 | 1.5 |
| Sarcina | 45 | 1.1 |
(22) And (3) taking 150mL of output liquid of the single well C to be tested, filling the output liquid into a 200mL anaerobic bottle, aseptically operating the agrobacterium fermentation liquid with the concentration of 2-10% and nutrient solution thereof, then placing the anaerobic bottle in an incubator to be cultured for 25d, setting the temperature of the incubator to be 20 ℃, observing the cellulose metabolism condition in the experimental process, measuring the dry weight of cellulose polysaccharide, and determining the most appropriate concentration of the cellulose polysaccharide-producing microorganisms. As is clear from Table 8, the effect of the Agrobacterium fermentation broth at a concentration of 10% was the best.
TABLE 8 effects of fermentation broth yield of Agrobacterium at different concentrations
| Cellulose microorganism | Concentration of bacteria (10)8one/mL) | Dry weight g/L of cellulose |
| 2％ | 47 | 0.9 |
| 4％ | 50 | 1.0 |
| 6％ | 62 | 1.4 |
| 8％ | 65 | 1.5 |
| 10％ | 70 | 1.6 |
(3) According to the growth requirement of the agrobacterium, screening out a proper activator formula (nutrition system):
150mL of output liquid of the test single well C is taken and filled into an anaerobic bottle with the volume of 200mL, nutrient solutions (3: 1; 10: 1; 20: 1; 25: 1) with different C/N ratios are prepared, then 10% concentration agrobacterium fermentation liquid is inoculated in the anaerobic bottle through aseptic operation, and then the anaerobic bottle is placed in an incubator to be cultured for 25 days, and the temperature of the incubator is set to be 20 ℃. And observing the bacterial concentration condition of the agrobacterium during the culture process, and determining the yield of the cellulose. As can be seen from Table 9, the Agrobacterium was selected to metabolize cellulose at a C/N ratio of 10: 1.
TABLE 9 cellulose metabolizing Effect of Geobacillus on different C/N ratios of nutrient solutions
| C/N ratio of nutrient solution | Concentration of bacteria (10)8one/mL) | Dry weight g of cellulose |
| 3:1 | 50 | 1.0 |
| 10:1 | 65 | 1.5 |
| 20:1 | 75 | 1.8 |
| 25:1 | 70 | 1.6 |
(4) The microorganism huff and puff injection process is formed according to the characteristic of high water content of a test single well C, and is mainly divided into two slugs for injection, and the specific process is as follows:
(41) before the microorganism injection construction, 20m is used3Washing the well with the hot water;
(42) two prepared 20m at wellhead3The liquid preparation pool is used for preparing the working fluid, the liquid preparation pool is used for injecting the working fluid into the test single well C, bacterial liquid (10% of agrobacterium fermentation liquid) is transported to a well head by a tank car, an activator formula with the CN ratio of 10:1 is prepared at the well head of the test single well C by using injection water, after the preparation, the bacterial liquid and the activator formula form a slug I and a slug II according to the volume ratios of 3:1 and 3:2 respectively, and the slug I and the slug II are injected quickly;
(43) (43) slug-displacement of 25m3H, construction pressure is controlled to be 12MPa, and total injection amount is 800m3；
The second displacement of the slug is 18m3H, construction pressure is controlled to be 12MPa, and total injection amount is 800m3；
Finally, 40m of implantation3Clear water is used as displacement liquid, and the injection speed is 20m3And h, closing the well and observing.
(44) And (3) observing the pressure change of the well mouth after closing the well, opening the well for production after the pressure does not rise any more and is kept for 5 days continuously, wherein the well closing time is 20 days, so that microorganisms are fully fermented and subjected to metabolic reaction underground, and the sleeve pressure and oil pressure change is observed during the well closing period.
(5) And (3) well opening production:
after the well is opened, the production dynamics is monitored, the water content is reduced to 58 percent, and the liquid amount is 15m310 tons of daily oil;
if the well-opening production liquid amount is low, the well-opening production liquid amount is less than 10m3One round of steam huff and puff or steam effect introduction can be carried out;
(6) evaluation of the effects:
the field test is carried out according to the injection process determined in the steps, the injection amount of 10 percent of the agrobacterium fermentation liquor and the activator in the volume ratio of 3:1 and 3:2 is 1600m3. And after the field test is finished, evaluating the effect of the field test, wherein the evaluation indexes comprise the oil increment, the validity period and the input-output ratio.
After field implementation, the single well C increases oil by 960 tons cumulatively, the validity period is 100 days, the input-output ratio is 1:4.2, and the field test effect is good.
The embodiments of the present invention have been described in detail. However, the present invention is not limited to the above-described embodiments, and various changes can be made within the knowledge of those skilled in the art without departing from the spirit of the present invention.
Claims (14)
1. A strong edge bottom water heavy oil reservoir microorganism huff and puff oil production method is characterized by comprising the following steps:
(1) screening a test oil reservoir;
(2) screening the cellulose polysaccharide-producing microorganisms and determining the most appropriate concentration of the cellulose polysaccharide-producing microorganisms;
(3) screening out a proper formula of the activating agent;
(4) carrying out field test;
(5) and opening the well for production;
(6) and evaluating the effect.
2. The microbial huff and puff oil recovery method for the strong bottom-edge water heavy oil reservoir as claimed in claim 1, wherein the screening criteria for testing the reservoir in step (1) are as follows:
selecting oil well with strong edge bottom water and thick oil, oil deposit temperature<50 ℃, the viscosity of crude oil is between 10000mPa.s and 30000mPa.s, and the liquid amount is 30-50m3And the water content of the produced liquid reaches 90-100%.
3. The microbial huff and puff oil recovery method for the strong bottom-edge water heavy oil reservoir as claimed in claim 1, wherein the step (2) comprises the following steps:
(21) taking 150ml of output liquid of the tested oil reservoir screened in the step (1), filling the output liquid into anaerobic bottles, respectively performing aseptic operation to access a plurality of cellulose polysaccharide-producing microorganisms and nutrient solution thereof, then placing the anaerobic bottles in a constant temperature box to be cultured for 15-30d, setting the temperature of the constant temperature box as the oil reservoir temperature of the tested oil reservoir, observing the cellulose metabolism condition in the experimental process, measuring the dry weight of cellulose polysaccharide, and screening the cellulose polysaccharide-producing microorganisms with the strongest cellulose polysaccharide-producing capability;
(22) and (2) filling 150ml of the output liquid of the test oil reservoir screened in the step (1) into an anaerobic bottle, carrying out aseptic operation on the cellulose-producing polysaccharide microorganism screened in the step (21) with the concentration of 2-10% and the nutrient solution thereof, then placing the anaerobic bottle in a constant temperature box for culturing for 15-30d, setting the temperature of the constant temperature box as the oil reservoir temperature of the test oil reservoir, observing the cellulose metabolism condition in the experimental process, measuring the dry weight of the cellulose polysaccharide, and determining the optimal concentration of the cellulose-producing polysaccharide microorganism.
4. The microbial huff and puff method for producing oil from strong bottom water heavy oil reservoirs of claim 3, wherein said cellulose-producing polysaccharide microbes comprise Acetobacter xylinum, Agrobacterium tumefaciens and sarcina.
5. The microbial huff and puff oil recovery method for strong bottom-water heavy oil reservoirs according to claim 4, wherein the formula of the nutrient solution of the acetic acid bacteria is 2-5% of glucose, 0.2-0.5% of yeast powder, 0.1-0.3% of dipotassium hydrogen phosphate and MgSO4·7H20.1 to 0.3 percent of O and the balance of water.
6. The microbial huff and puff oil recovery method for strong bottom-water heavy oil reservoirs according to claim 4, wherein the nutrient solution formula of the agrobacterium is fructose 2-4%, urea 0.1-0.3%, yeast powder 0.1-0.2%, K2HPO4 0.1-0.4％、FeSO4·7H20.01 to 0.02 percent of O and the balance of water.
7. The microbial huff and puff oil recovery method for strong bottom-edge water heavy oil reservoirs according to claim 4, wherein the nutrient solution formula of sarcina is 3-5% of molasses, 0.2-0.8% of ammonium nitrate, 0.2-0.4% of dipotassium hydrogen phosphate, 0.1-0.3% of potassium dihydrogen phosphate, 0.03-0.1% of sodium chloride and the balance of water.
8. The microbial huff and puff oil recovery method for the strong bottom-edge water heavy oil reservoir as claimed in claim 1, wherein the step (3) comprises the following steps:
and (2) filling 150ml of produced liquid of the tested oil reservoir screened in the step (1) into an anaerobic bottle, and configuring the C/N ratio to be (3-25): 1, inoculating the nutrient solution of the cellulose-producing polysaccharide microorganism screened in the step (2) into the cellulose-producing polysaccharide microorganism fermentation liquor with the optimal concentration determined in the step (2) through aseptic operation, placing an anaerobic bottle in a constant temperature box to culture for 15-30 days at the oil reservoir temperature, observing the bacterial concentration condition in the culture process, determining the cellulose yield, and further determining the optimal C/N ratio.
9. The microbial huff and puff oil recovery method for the strong bottom-edge water heavy oil reservoir as claimed in claim 1, wherein the step (4) comprises the following steps:
(41) before the construction of injecting cellulose polysaccharide-producing microorganism, 20m is used3Washing the well with the hot water;
(42) cellulose-producing polysaccharide bacterial liquid and injection of nutrient solution thereof
(421) Respectively configuring a proper activator formula (nutrient solution) determined in the step (3) and the cellulose-producing polysaccharide microorganism fermentation liquor with the optimal concentration determined in the step (2) at a wellhead of a test oil reservoir, then forming a first slug and a second slug by the configured proper activator formula (nutrient solution) determined in the step (3) and the cellulose-producing polysaccharide microorganism fermentation liquor with the optimal concentration according to the volume ratio of 3:1 and 3:2, and quickly injecting the slugs into the wellhead of the test oil reservoir, wherein:
the first displacement of the slug is 20m3Over h, construction pressure controlled at 12-20 MPa, total injection amount 400m3～800m3；
The second displacement of the slug is 15m3Over h, construction pressure controlled at 12-20 MPa, total injection amount 400m3～800m3；
(422) Injecting 15-40 m into the wellhead of the test oil reservoir3Using clear water as displacement liquid, and closing the well;
(433) and (5) observing the pressure change of the well mouth after closing the well, and entering the step (5) when the pressure does not rise any more and is kept for 5 days continuously.
10. The microbial huff and puff oil recovery method for strong bottom water heavy oil reservoirs of claim 9, wherein in the step (421), the proper activator formulation is prepared at the well head by using the injected water, and the fermentation broth of the cellulose-producing polysaccharide microorganism with proper concentration is transported to the well head by a tanker truck.
11. The microbial stimulation oil recovery method for the strong bottom water heavy oil reservoir of claim 9, wherein in the step (421), two fluid preparation pools are prepared at the wellhead of the test reservoir, one fluid preparation pool is used for preparing the well fluid, and the other fluid preparation pool is used for injecting the well fluid into the wellhead.
12. The microbial stimulation oil recovery method for the strong edge-bottom water heavy oil reservoir as claimed in claim 11, wherein the volumes of the two liquid preparation pools in the step (421) are both 20m3。
13. The microbial huff and puff oil recovery method for heavy oil reservoirs with strong bottom water according to claim 1, wherein in the step (5), if the well-opening production fluid volume is low, the well-opening production fluid volume is less than 10m3One steam stimulation or steam induction cycle can be performed.
14. The microbial huff and puff oil recovery method for the strong bottom-edge water heavy oil reservoir as claimed in claim 1, wherein the indexes of the field test effect evaluation in the step (6) comprise oil increment, validity period and input-output ratio.
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