CN114414488B - Nondestructive blood bag plasma chyle index measurement method - Google Patents
Nondestructive blood bag plasma chyle index measurement method Download PDFInfo
- Publication number
- CN114414488B CN114414488B CN202210079537.6A CN202210079537A CN114414488B CN 114414488 B CN114414488 B CN 114414488B CN 202210079537 A CN202210079537 A CN 202210079537A CN 114414488 B CN114414488 B CN 114414488B
- Authority
- CN
- China
- Prior art keywords
- plasma
- index
- chylomicron
- blood bag
- light intensity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/59—Transmissivity
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention provides a nondestructive blood bag plasma chyle index measurement method, which comprises the following steps: acquiring a first light intensity value of the light source after penetrating through the blood bag with a first thickness value h1The method comprises the steps of carrying out a first treatment on the surface of the Acquiring a second light intensity value of the light source after penetrating through the blood bag with the second thickness value h2The method comprises the steps of carrying out a first treatment on the surface of the According to the first light intensity valueA second light intensity valueCalculating the chylomicron index of the plasma; wherein the second thickness value h2 is smaller than the first thickness value h1. The invention does not need to extract the blood plasma from the blood bag to measure the chylomicron index, and solves the problems that the existing blood bag blood plasma chylomicron index measuring method needs to extract part of the blood plasma from the blood bag to measure, and the problems of plasma loss and secondary pollution risks exist.
Description
Technical Field
The invention relates to the technical field of material analysis, in particular to a nondestructive measurement method for blood bag plasma chyle index.
Background
The chylomicron index of plasma is an important parameter that determines whether plasma can be used clinically, and excessive chylomicron is not suitable for clinical use. For the measurement of chyle index of blood bag plasma, the existing methods all need to extract part of plasma from the blood bag, then the extracted plasma is injected into transparent containers with fixed shapes, and finally the methods of index method, nephelometry, enzyme-labeled nephelometry, full-automatic enzyme-immune instrument judgment method, biochemical analyzer judgment method, CCD-based plasma chyle detection and the like are adopted for measurement.
However, the existing blood bag plasma chylomicron index measurement method needs to draw part of plasma from the blood bag for measurement, and the plasma loss and secondary pollution risks exist.
Disclosure of Invention
Based on the above, in order to solve the problems that the existing blood bag plasma chylomicron index measurement method needs to be used for measuring by extracting part of plasma from the blood bag, and the plasma loss and the risk of secondary pollution exist, the invention provides a nondestructive blood bag plasma chylomicron index measurement method, which has the following specific technical scheme:
a non-destructive blood bag plasma chylomicron index measurement method, comprising the following steps:
s1, acquiring a first light intensity value of the light source penetrating through the blood bag with a first thickness value h1。
S2, obtaining a second light intensity value of the light source penetrating through the blood bag with a second thickness value h2。
S3, according to the first light intensity valueAnd said second light intensity value +.>The chylomicron index of the plasma was calculated.
The non-destructive blood bag plasma chylomicron index measurement method does not need to extract plasma from the blood bag to measure the chylomicron index, and solves the problems that the existing blood bag plasma chylomicron index measurement method needs to extract part of plasma from the blood bag to measure, and the problems of plasma loss and secondary pollution exist.
Wherein the second thickness value h2 is smaller than the first thickness value h1.
Further, the chylomicron index of the plasmaWherein->，,/>A third light intensity value of light source after passing through plasma with chylomicron index of 1 with first thickness value h1, ">A fourth light intensity value obtained after the light source transmits the plasma with the chylomicron index of 1 with the second thickness value h 2.
Wherein, the plasma with the chyle index of 1 is expressed as 1:1 ratio is diluted by colorless transparent physiological saline, and the blood lipid concentration is changed into the original concentrationThe post-light transmittance is the same as the normal plasma transmittance.
Further, the chylomicron index of the plasmaWherein->，/>,/>A third light intensity value obtained after the light source transmits the plasma with the chyle index of 1 of the first thickness value h1,a fourth light intensity value obtained after the light source transmits the plasma with the chylomicron index of 1 with the second thickness value h 2.
Wherein, the plasma slurry with the chyle index of 1 is expressed as 1:2, the concentration of blood fat is changed into the original concentration by dilution with colorless and transparent normal salineThe post-light transmittance is the same as the normal plasma transmittance.
Further, a light intensity signal is generated through the light sensor, and the light intensity signal of the light source penetrating through the blood bag with the first thickness value h1 is converted into a first light intensity value through the control module, and the light intensity signal of the light source penetrating through the blood bag with the second thickness value h2 is converted into a second light intensity value.
Further, the blood bag is located between the light source and the light sensor.
Further, a light filter is arranged between the blood bag and the light sensor.
Further, the light source is a monochromatic light source.
A computer readable storage medium storing a computer program which when executed by a processor implements the method of non-destructive blood bag plasma chylomicron index measurement.
Drawings
The invention will be further understood from the following description taken in conjunction with the accompanying drawings. The components in the figures are not necessarily to scale, emphasis instead being placed upon illustrating the principles of the embodiments. Like reference numerals designate corresponding parts throughout the different views.
FIG. 1 is a schematic overall flow chart of a non-invasive blood bag plasma chylomicron index measurement method according to an embodiment of the invention.
Detailed Description
The present invention will be described in further detail with reference to the following examples thereof in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the detailed description and specific examples are intended for purposes of illustration only and are not intended to limit the scope of the invention.
It will be understood that when an element is referred to as being "fixed to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only and are not meant to be the only embodiment.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
The terms "first" and "second" in this specification do not denote a particular quantity or order, but rather are used for distinguishing between similar or identical items.
As shown in fig. 1, a non-destructive blood bag plasma chylomicron index measurement method according to an embodiment of the invention includes the following steps:
s1, acquiring a first light intensity value of the light source penetrating through the blood bag with a first thickness value h1。
S2, obtaining a second light intensity value of the light source penetrating through the blood bag with a second thickness value h2。
S3, according to the first light intensity valueAnd said second light intensity value +.>The chylomicron index of the plasma was calculated.
Wherein the second thickness value h2 is smaller than the first thickness value h1.
Because the difference of blood lipid concentration of blood bag plasma at the first thickness value and the second thickness value leads to the difference of light intensity signals generated by the light sensor twice, the blood bag plasma can be obtained according to the lambert-beer lawEither->. Wherein (1)>Is molar extinction coefficient of blood lipid, < >>Is the concentration of the blood fat,representative is the light attenuation of the monochromatic light of the light source due to the plasma of the thickness h1-h 2. Due to->And h1, h2 are fixed, thus->Or->Just the concentration of blood fat>Related to (I)>And->In a linear relationship.
Assume that the concentration of blood lipid in normal plasma isIt is known from the standard of GB 18469-2001 that if the plasma to be tested is measured at a ratio of 1: n ratio and diluted with colorless transparent physiological saline to change blood lipid concentration into original +.>The post light transmittance is the same as that of normal blood plasma, and the chylomicron index of the blood plasma to be measured is n. It can be deduced that when the blood plasma lipid concentration to be measured is (1+n)/(1)>The chylomicron index of the plasma to be measured is n. Thus, the following relationship table of blood lipid concentration and chylomicron index can be obtained.
Assuming that the calibration was performed using plasma with a chylomicron index of 1, the light attenuation measured at this time was recorded asSince the light attenuation is linearly related to the blood lipid concentration, the following table of the relationship between the light attenuation and the chyle index can be obtained.
As can be seen from the above table, the chylomicron index isThe light attenuation at the time is +.>。
From this it can be derived that the chylomicron index of the plasma. Wherein (1)>，,/>A third light intensity value of light source after passing through plasma with chylomicron index of 1 with first thickness value h1, ">A fourth light intensity value obtained after the light source transmits the plasma with the chylomicron index of 1 with the second thickness value h 2.
Regarding the correspondence of chyle index and dilution factor, the following method is also adopted in some places: dilution ratio 1:2 is 1, the dilution ratio is 1:4 corresponds to a chylomicron index of 2, dilution ratio 1:8 corresponds to a chylomicron index of 3, dilution ratio 1:16 corresponds to a chylomicron index of 4, dilution ratio 1:32 corresponds to a chylomicron index of 5, dilution ratio 1:64 corresponds to a chylomicron index of 6.
Suppose that the blood lipid concentration in normal blood plasma isAccording to this correspondence, a table of the relationship between the blood lipid concentration and the chylomicron index of # 2 can be obtained.
Assuming that the calibration was performed using plasma with a chylomicron index of 1 # 2, the light attenuation measured at this time was recorded asSince the light attenuation is linearly related to the blood lipid concentration, the following relationship between the light attenuation and the No. 2 chyle index can be obtainedIs a table.
As can be seen from the above table, the No. 2 chyle index isThe light attenuation at the time is +.>。
From this, it can be derived that the plasma has a chylomicron index of 2#Wherein, the method comprises the steps of, wherein,，/>,/>a third light intensity value of light source after passing through plasma with a 2# chylomicron index of 1 with a first thickness value h1, ">A fourth light intensity value obtained after the light source transmits the plasma with the 2# chyle index of 1 with the second thickness value h 2.
In summary, the method for measuring the chylomicron index of the blood plasma in the blood bag is capable of solving the problems that the prior method for measuring the chylomicron index of the blood plasma in the blood bag needs to measure the blood plasma in the blood bag by extracting part of the blood plasma from the blood bag, and has the risks of plasma loss and secondary pollution by utilizing the light transmission characteristic difference of the blood plasma blood bag at different thickness and the blood plasma chylomicron index calculated according to the first light intensity value and the second light intensity value.
In one embodiment, the light intensity signal is generated by the light sensor, and the light intensity signal of the light source after passing through the blood bag with the first thickness value h1 is converted into a first light intensity value by the control module, and the light intensity signal of the light source after passing through the blood bag with the second thickness value h2 is converted into a second light intensity value.
In one embodiment, a filter is provided between the blood bag and the light sensor. Specifically, the filter is in close proximity to the light sensor. Through the optical filter, light interference outside the light source wave band can be avoided, and the measurement accuracy of the blood bag plasma chyle index is improved.
In one embodiment, the blood bag is located between the light source and the light sensor, the light source being a monochromatic light source. Thus, the measurement sensitivity of the blood bag plasma chylomicron index can be improved.
In one embodiment, the two sides of the blood bag are respectively clung to the light source and the optical filter (when the optical filter is not included, the two sides of the blood bag are respectively clung to the light source and the optical sensor), and the blood bag in the measurement area is filled with plasma without bubbles, so that the measurement accuracy of the chylomicron index of the plasma is improved. h2 and h1 have obvious thickness difference, so that the first light intensity value and the second light intensity value obtained by two measurements have obvious difference, and the chylomicron index of the plasma is measured better.
A computer readable storage medium storing a computer program which when executed by a processor implements the method of non-destructive blood bag plasma chylomicron index measurement.
The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The above examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (6)
1. The nondestructive blood bag plasma chyle index measurement method is characterized by comprising the following steps of:
acquiring a first light intensity value of the light source penetrating through the blood bag with a first thickness value h1；
Acquiring a second light intensity value of the light source penetrating through the blood bag with a second thickness value h2；
According to the first light intensity valueAnd said second light intensity value +.>Calculating the chylomicron index of the plasma;
wherein the second thickness value h2 is smaller than the first thickness value h1;
chylomicron index of the plasmaWherein->，/>,/>A third light intensity value of light source after passing through plasma with chylomicron index of 1 with first thickness value h1, ">A fourth light intensity value after the light source transmits through the plasma with the chylomicron index of 1 of the second thickness value h2, wherein the plasma with the chylomicron index of 1 refers to a plasma with the chylomicron index of 1:1 ratio is diluted by colorless transparent physiological saline, and the blood lipid concentration is changed into the original +.>Plasma having the same post-light transmittance as normal plasma light transmittance;
or the chylomicron index of the plasmaWherein->，,/>A third light intensity value obtained after the light source transmits the plasma with the chyle index of 1 of the first thickness value h1,a fourth light intensity value after the light source transmits through the plasma with the chylomicron index of 1 of the second thickness value h2, wherein the plasma with the chylomicron index of 1 refers to a plasma with the chylomicron index of 1:2 ratio is diluted by colorless transparent physiological saline, and the blood lipid concentration is changed into the original +.>The post-light transmittance is the same as the normal plasma transmittance.
2. The method of claim 1, wherein a light intensity signal is generated by a light sensor, and the light intensity signal of the light source passing through the blood bag with a first thickness value h1 is converted into a first light intensity value and the light intensity signal of the light source passing through the blood bag with a second thickness value h2 is converted into a second light intensity value by a control module.
3. A non-destructive blood bag plasma chylomicron index measurement method as in claim 2, wherein the blood bag is positioned between the light source and the light sensor.
4. A non-destructive blood bag plasma chylomicron index measurement method as in claim 3, wherein an optical filter is disposed between the blood bag and the light sensor.
5. The method of claim 4, wherein the light source is a monochromatic light source.
6. A computer readable storage medium, characterized in that the computer readable storage medium stores a computer program, which when executed by a processor implements the non-destructive blood bag plasma chylomicron index measurement method of any one of claims 1-5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210079537.6A CN114414488B (en) | 2022-01-24 | 2022-01-24 | Nondestructive blood bag plasma chyle index measurement method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210079537.6A CN114414488B (en) | 2022-01-24 | 2022-01-24 | Nondestructive blood bag plasma chyle index measurement method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114414488A CN114414488A (en) | 2022-04-29 |
| CN114414488B true CN114414488B (en) | 2023-08-01 |
Family
ID=81278294
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210079537.6A Active CN114414488B (en) | 2022-01-24 | 2022-01-24 | Nondestructive blood bag plasma chyle index measurement method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114414488B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07280814A (en) * | 1994-04-14 | 1995-10-27 | Hitachi Ltd | Automatic specimen inspection system |
| CN1560599A (en) * | 2004-03-01 | 2005-01-05 | 北京市红十字血液中心 | Method for investigating blood chylus degree |
| JP2008026036A (en) * | 2006-07-18 | 2008-02-07 | Aloka Co Ltd | Inclusion measuring device |
| CN105092474A (en) * | 2015-08-11 | 2015-11-25 | 中山大学 | Device and method for measuring extinction coefficient of water body and method for measuring extinction coefficient of suspended matter |
| CN214622293U (en) * | 2021-04-09 | 2021-11-05 | 深圳市迈思特生物医学工程有限公司 | Blood detection device |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6995835B2 (en) * | 1997-03-03 | 2006-02-07 | Nir Diagnostics Inc. | Method and apparatus for measuring analytes in blood bags |
| US20020186363A1 (en) * | 1999-09-03 | 2002-12-12 | James Samsoondar | Method and apparatus for screening plasma for interferents in plasma from donor blood bags |
| CN201993312U (en) * | 2011-01-31 | 2011-09-28 | 山东省血液中心 | Fat blood plasma chyle degree detection device |
| JP5474903B2 (en) * | 2011-09-28 | 2014-04-16 | あおい精機株式会社 | Pre-test processing apparatus, pre-test processing method, and sample processing apparatus |
| CN103207157A (en) * | 2013-04-11 | 2013-07-17 | 侯广安 | Rapid detection method for severe chylemia |
| CN206563707U (en) * | 2017-03-20 | 2017-10-17 | 山东省血液中心 | Blood plasma chyle interpretoscope and sampled plasma system |
| CN108760659B (en) * | 2018-08-16 | 2024-03-22 | 济南希望医疗器械有限公司 | Primary blood donation analyzer and use method thereof |
| CN109632654B (en) * | 2019-01-23 | 2022-04-05 | 南方医科大学南方医院 | Plasma chyle and hemolysis detection device and implementation method thereof |
| CN111829962B (en) * | 2020-06-28 | 2023-03-28 | 中国科学院苏州生物医学工程技术研究所 | Plasma chyle degree detection method and system |
-
2022
- 2022-01-24 CN CN202210079537.6A patent/CN114414488B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07280814A (en) * | 1994-04-14 | 1995-10-27 | Hitachi Ltd | Automatic specimen inspection system |
| CN1560599A (en) * | 2004-03-01 | 2005-01-05 | 北京市红十字血液中心 | Method for investigating blood chylus degree |
| JP2008026036A (en) * | 2006-07-18 | 2008-02-07 | Aloka Co Ltd | Inclusion measuring device |
| CN105092474A (en) * | 2015-08-11 | 2015-11-25 | 中山大学 | Device and method for measuring extinction coefficient of water body and method for measuring extinction coefficient of suspended matter |
| CN214622293U (en) * | 2021-04-09 | 2021-11-05 | 深圳市迈思特生物医学工程有限公司 | Blood detection device |
Non-Patent Citations (1)
| Title |
|---|
| 低温倒置静置法降低乳糜血浆报废率的研究应用;罗怀雪，等;中国实用医药(第21期);198-199 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114414488A (en) | 2022-04-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1699973B (en) | Method for realizing concentration measurement by employing flotation benchmarks | |
| Davydov et al. | An optical method of monitoring the state of flowing media with low transparency that contain large inclusions | |
| CN103399006B (en) | Color RGB (red, green and blue)-component-based urine analysis device and processing method thereof | |
| Maisels et al. | Transcutaneous bilirubin measurements in full-term infants | |
| CN105572386A (en) | Kit for detecting heparin binding protein through immunofluorescence chromatography and preparation method of kit | |
| CN202720235U (en) | Quantitative detection device for retinol conjugated proteins | |
| CN109459574A (en) | For detecting the immuno-chromatographic test paper strip of saccharification hemoglobin content and comprising its immunoassay detection device | |
| US20200271590A1 (en) | Rapid joint detection device and detection method for deterioration degree of fried oil | |
| CN112485438B (en) | Specific protein reaction detection method and device | |
| CN114414488B (en) | Nondestructive blood bag plasma chyle index measurement method | |
| CN209400549U (en) | For detecting the immuno-chromatographic test paper strip of saccharification hemoglobin content and comprising its immunoassay detection device | |
| CN113176230B (en) | Erythrocyte identification and attitude estimation method based on microfluidic lens-free imaging | |
| CN207366571U (en) | A kind of gold mark and fluorescence immune chromatography synthesis analyzer | |
| CN207366368U (en) | A kind of fruit quality comprehensive detection analyzer | |
| CN204177810U (en) | Plasma proteins quick analysis system | |
| CN103837686A (en) | Kit for detecting human immunoglobulin G4 as well as preparation method of kit | |
| CN109115747B (en) | System and method for measuring glass material properties based on Raman spectrum and OCT | |
| CN101451948A (en) | Quantitative determination device by gold marking spot method based on digital picture treatment | |
| CN204882371U (en) | Digital photoelectricity biosensor | |
| Tomassetti et al. | Validation of the New MINI-CUBE for Clinic Determination of Erythrocyte Sedimentation Rate | |
| CN203178021U (en) | Pressure measuring system based on compensated-type micromanometer | |
| CN208060369U (en) | Total chromium water quality on-line checking analyzer | |
| CN101852721A (en) | Device for detecting concentration of transparent mixed solution with two solutes | |
| CN206321344U (en) | A kind of spectrum sensor | |
| CN104856656B (en) | Fiber grating pulse test head, three road optics pulse test system and methods |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |