CN114410719A - Method for efficiently recovering proteins in shrimp shells - Google Patents
Method for efficiently recovering proteins in shrimp shells Download PDFInfo
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- CN114410719A CN114410719A CN202111665732.9A CN202111665732A CN114410719A CN 114410719 A CN114410719 A CN 114410719A CN 202111665732 A CN202111665732 A CN 202111665732A CN 114410719 A CN114410719 A CN 114410719A
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- shrimp
- shrimp shell
- powder
- protein
- water
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 62
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 62
- 241000238557 Decapoda Species 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 38
- 239000000843 powder Substances 0.000 claims abstract description 77
- MBLBDJOUHNCFQT-LXGUWJNJSA-N aldehydo-N-acetyl-D-glucosamine Chemical compound CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 claims abstract description 71
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 53
- 239000004576 sand Substances 0.000 claims abstract description 39
- 108010009736 Protein Hydrolysates Proteins 0.000 claims abstract description 24
- 230000007062 hydrolysis Effects 0.000 claims abstract description 24
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 24
- 239000003531 protein hydrolysate Substances 0.000 claims abstract description 24
- 108091005658 Basic proteases Proteins 0.000 claims abstract description 22
- 239000002002 slurry Substances 0.000 claims abstract description 20
- 238000010438 heat treatment Methods 0.000 claims abstract description 18
- 238000003801 milling Methods 0.000 claims abstract description 18
- 238000001035 drying Methods 0.000 claims abstract description 17
- 239000000203 mixture Substances 0.000 claims abstract description 15
- 102000004190 Enzymes Human genes 0.000 claims abstract description 11
- 108090000790 Enzymes Proteins 0.000 claims abstract description 11
- 238000001816 cooling Methods 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 9
- 239000002245 particle Substances 0.000 claims abstract description 9
- 238000005406 washing Methods 0.000 claims abstract description 9
- 238000001694 spray drying Methods 0.000 claims abstract description 3
- 238000000227 grinding Methods 0.000 abstract description 3
- 238000011084 recovery Methods 0.000 abstract description 3
- 238000000643 oven drying Methods 0.000 abstract 1
- 238000003756 stirring Methods 0.000 description 7
- OHJMTUPIZMNBFR-UHFFFAOYSA-N biuret Chemical compound NC(=O)NC(N)=O OHJMTUPIZMNBFR-UHFFFAOYSA-N 0.000 description 6
- 230000003301 hydrolyzing effect Effects 0.000 description 6
- 238000009210 therapy by ultrasound Methods 0.000 description 6
- 230000007071 enzymatic hydrolysis Effects 0.000 description 5
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 5
- 229920002101 Chitin Polymers 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 241000238017 Astacoidea Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000238559 Macrobrachium Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Abstract
The invention discloses a method for efficiently recovering proteins in shrimp shells, and belongs to the technical field of protein recovery. The method comprises (1) repeatedly washing shrimp shell with running water, and oven drying; then crushing the mixture to obtain primary shrimp shell powder with the particle size of 0.125-0.150 mm; (2) adding the shrimp shell primary powder and water into an ultrasonic stirrer, and uniformly mixing to obtain slurry; adding the slurry into a sand mill for sand milling treatment, and drying after sand milling to obtain shrimp shell powder; (3) adding water and alkaline protease into the shrimp shell powder for hydrolysis, heating to 100 ℃ after hydrolysis, heating in water bath to inactivate enzyme, and cooling to obtain protein hydrolysate; (4) and spray drying the obtained protein hydrolysate to obtain the protein powder. The method adopts sand grinding treatment to shrimp shell to obtain shrimp shell powder, and then performs enzyme hydrolysis treatment, so as to reduce the dosage of alkaline protease during enzyme hydrolysis and effectively improve the protein yield.
Description
Technical Field
The invention belongs to the technical field of protein recovery, and particularly relates to a method for efficiently recovering proteins in shrimp shells.
Background
At present, the culture and processing of various shrimps such as macrobrachium, prawns, crayfish and the like in China become the first major country in the world, the main variety is 1500 ten thousand mu of water surface, and the total yield of the shrimps is more than 250 ten thousand tons. The processing level of shrimps is continuously improved, the varieties are continuously enriched, the international competitiveness is obviously enhanced, the industrial chain and the industrial state are gradually optimized, but a plurality of problems still exist in the aspect of the matched development of byproduct recycling.
The method is not suitable for industrial clusters, the development of the matched processing industry is lagged, the amount of wastes is large, and secondary pollution is easily accumulated; the traditional treatment mode mainly selectively utilizes chitin components, the residual proportion of organic biomass is more than 55 percent, the high-value processing is not realized, the research and development of green and biological precise deep processing technologies are urgently needed, novel high-value deep processing products are developed, and an industrial new mode of resource full-value utilization is constructed.
The shrimp processing by-products mainly comprise shrimp heads and shrimp shells and contain chitin, protein, fat, organic calcium and other components, the existing process for recovering the protein by the enzymatic hydrolysis of the shrimp shells is simple, the protein recovery rate is not high, the protease consumption is high, the cost is high, and the industrial production cannot be realized.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a method for efficiently recovering proteins in shrimp shells, and the method adopts sand grinding treatment on the shrimp shells to obtain shrimp shell powder, and then carries out enzymatic hydrolysis treatment, so that the dosage of alkaline protease in enzymatic hydrolysis can be reduced, and the protein yield is effectively improved.
In order to solve the problems, the technical scheme adopted by the invention is as follows:
a method for efficiently recovering proteins in shrimp shells comprises the following steps:
(1) repeatedly washing shrimp shells with running water, and drying; then crushing the mixture to obtain primary shrimp shell powder with the particle size of 0.125-0.150 mm;
(2) adding the shrimp shell primary powder and water into an ultrasonic stirrer, and uniformly mixing to obtain slurry; adding the slurry into a sand mill for sand milling treatment, and drying after sand milling to obtain shrimp shell powder;
(3) adding water and alkaline protease into the shrimp shell powder for hydrolysis, heating to 100 ℃ after hydrolysis, heating to inactivate enzyme, and cooling to obtain protein hydrolysate;
(4) and spray drying the obtained protein hydrolysate to obtain the protein powder.
The method for efficiently recovering the protein in the shrimp shells comprises the step (2), wherein the mass ratio of the primary powder of the shrimp shells to water is 0.1-1.5: 1.
The method for efficiently recovering the protein in the shrimp shells comprises the step (2), wherein the mass ratio of the primary powder of the shrimp shells to water is 0.8-1.2: 1.
The method for efficiently recovering the protein in the shrimp shells has the ultrasonic power of 20-150 KW and the ultrasonic time of 0.5-3 h.
The method for efficiently recovering the protein in the shrimp shells has the advantages that the sand mill is kept at a constant speed when working, the speed of the sand mill is 1000-3500r/min, and the sanding time is 1-5 h.
The method for efficiently recovering the protein in the shrimp shells has the advantages that the sand mill is kept at a constant speed when in work, the speed of the sand mill is 1500-.
The method for efficiently recovering the protein in the shrimp shells comprises the step (3), wherein the mass ratio of the shrimp shell powder to water is 0.5:1, the dosage of the alkaline protease is 0.5-1.5IU/g, and the hydrolysis time is 0.5-1.5 h.
The method for efficiently recovering the protein in the shrimp shells comprises the step (3), wherein the dosage of the alkaline protease is 0.8-1.2IU/g, and the hydrolysis time is 1.0 h.
Has the advantages that: compared with the prior art, the invention has the advantages that:
according to the invention, the shrimp shell slurry is obtained by ultrasonic stirring, then the shrimp shell slurry is subjected to sand grinding treatment, and after the combined treatment, the shrimp shell is subjected to enzymatic hydrolysis treatment, so that the dosage of alkaline protease in enzymatic hydrolysis can be reduced, and the protein yield is effectively improved.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with examples are described in detail below.
Example 1
A method for efficiently recovering proteins in shrimp shells comprises the following steps:
(1) repeatedly washing shrimp shells with running water, and drying; then crushing the mixture to obtain primary shrimp shell powder with the particle size of 0.125-0.15 mm;
(2) adding the shrimp shell primary powder and water into an ultrasonic stirrer, and carrying out ultrasonic treatment for 1.0h under the condition of 20KW power to uniformly mix the shrimp shell primary powder and the water to obtain slurry, wherein the mass ratio of the shrimp shell primary powder to the water is 0.5:1 during ultrasonic stirring and mixing; directly transferring the obtained slurry into a sand mill, performing sand milling treatment for 2 hours at a sand milling speed of 1500r/min, and drying to obtain shrimp shell powder;
(3) adding water and alkaline protease into the shrimp shell powder for hydrolysis for 1.0h, wherein the mass ratio of the shrimp shell powder to the water is 0.5:1, and the dosage of the alkaline protease is 0.5 IU/g; after the hydrolysis is finished, heating to 100 ℃, heating to inactivate the enzyme, and cooling to obtain protein hydrolysate;
(4) the protein concentration in the protein hydrolysate is measured by a biuret method, and the total amount of protein is calculated, so that the result shows that 233.65 mg of protein can be obtained by hydrolyzing the shrimp shell primary powder per gram after the treatment, and then the protein hydrolysate is spray-dried to obtain the protein powder.
Example 2
A method for efficiently recovering proteins in shrimp shells comprises the following steps:
(1) repeatedly washing shrimp shells with running water, and drying; then crushing the mixture to obtain primary shrimp shell powder with the particle size of 0.125-0.15 mm;
(2) adding the shrimp shell primary powder and water into an ultrasonic stirrer, and carrying out ultrasonic treatment for 1.0h under the condition of 50KW power to uniformly mix the shrimp shell primary powder and the water to obtain slurry, wherein the mass ratio of the shrimp shell primary powder to the water is 0.8:1 during ultrasonic stirring and mixing; directly transferring the obtained slurry into a sand mill, performing sand milling treatment for 3.5h at a sand milling speed of 1500r/min, and drying to obtain shrimp shell powder;
(3) adding water and alkaline protease into the shrimp shell powder for hydrolysis for 1.0h, wherein the mass ratio of the shrimp shell powder to the water is 0.5:1, and the dosage of the alkaline protease is 0.5 IU/g; after the hydrolysis is finished, heating to 100 ℃, heating to inactivate the enzyme, and cooling to obtain protein hydrolysate;
(4) the protein concentration in the protein hydrolysate is measured by a biuret method, and the total amount of protein is calculated, so that the result shows that 252.81 mg of protein can be obtained by hydrolyzing the shrimp shell primary powder per gram after the treatment, and then the protein hydrolysate is spray-dried to obtain the protein powder.
Example 3
A method for efficiently recovering proteins in shrimp shells comprises the following steps:
(1) repeatedly washing shrimp shells with running water, and drying; then crushing the mixture to obtain primary shrimp shell powder with the particle size of 0.125-0.15 mm;
(2) adding the shrimp shell primary powder and water into an ultrasonic stirrer, and carrying out ultrasonic treatment for 2.5 hours under the condition of 85KW power to uniformly mix the shrimp shell primary powder and the water to obtain slurry, wherein the mass ratio of the shrimp shell primary powder to the water is 1.0:1 during ultrasonic stirring and mixing; directly transferring the obtained slurry into a sand mill, performing sand milling treatment for 4.0h at a sand milling speed of 3000r/min, and drying to obtain shrimp shell powder;
(3) adding water and alkaline protease into the shrimp shell powder for hydrolysis for 1.0h, wherein the mass ratio of the shrimp shell powder to the water is 0.5:1, and the dosage of the alkaline protease is 0.5 IU/g; after the hydrolysis is finished, heating to 100 ℃, heating to inactivate the enzyme, and cooling to obtain protein hydrolysate;
(4) the protein concentration in the protein hydrolysate is measured by a biuret method, and the total amount of protein is calculated, so that the result shows that 217.67 mg of protein can be obtained by hydrolyzing the shrimp shell primary powder per gram after the treatment, and then the protein hydrolysate is spray-dried to obtain the protein powder.
Example 4
A method for efficiently recovering proteins in shrimp shells comprises the following steps:
(1) repeatedly washing shrimp shells with running water, and drying; then crushing the mixture to obtain primary shrimp shell powder with the particle size of 0.125-0.15 mm;
(2) adding the shrimp shell primary powder and water into an ultrasonic stirrer, and carrying out ultrasonic treatment for 0.5h under the condition of 150KW power to uniformly mix the shrimp shell primary powder and the water to obtain slurry, wherein the mass ratio of the shrimp shell primary powder to the water is 1.2:1 during ultrasonic stirring and mixing; directly transferring the obtained slurry into a sand mill, performing sand milling treatment at a sand milling speed of 1500r/min for 4.0h, and drying to obtain shrimp shell powder;
(3) adding water and alkaline protease into the shrimp shell powder for hydrolysis for 0.5h, wherein the mass ratio of the shrimp shell powder to the water is 0.5:1, and the dosage of the alkaline protease is 1.2 IU/g; after the hydrolysis is finished, heating to 100 ℃, heating to inactivate the enzyme, and cooling to obtain protein hydrolysate;
(4) the protein concentration in the protein hydrolysate is measured by a biuret method, and the total amount of protein is calculated, so that the result shows that 259.13 mg of protein can be obtained by hydrolyzing the shrimp shell primary powder per gram after the treatment, and then the protein hydrolysate is spray-dried to obtain the protein powder.
Example 5
A method for efficiently recovering proteins in shrimp shells comprises the following steps:
(1) repeatedly washing shrimp shells with running water, and drying; then crushing the mixture to obtain primary shrimp shell powder with the particle size of 0.125-0.15 mm;
(2) adding the shrimp shell primary powder and water into an ultrasonic stirrer, and carrying out ultrasonic treatment for 0.5h under the condition of 150KW power to uniformly mix the shrimp shell primary powder and the water to obtain slurry, wherein the mass ratio of the shrimp shell primary powder to the water is 1.2:1 during ultrasonic stirring and mixing; directly transferring the obtained slurry into a sand mill, performing sand milling treatment for 4.5h at a sand milling speed of 3000r/min, and drying to obtain shrimp shell powder;
(3) adding water and alkaline protease into the shrimp shell powder for hydrolysis for 1.5h, wherein the mass ratio of the shrimp shell powder to the water is 0.5:1, and the dosage of the alkaline protease is 1.5 IU/g; after the hydrolysis is finished, heating to 100 ℃, heating to inactivate the enzyme, and cooling to obtain protein hydrolysate;
(4) the protein concentration in the protein hydrolysate is measured by a biuret method, and the total amount of protein is calculated, so that the result shows that 248.92 mg of protein can be obtained by hydrolyzing the shrimp shell primary powder per gram after the treatment, and then the protein hydrolysate is spray-dried to obtain the protein powder.
Example 5
A method for efficiently recovering proteins in shrimp shells comprises the following steps:
(1) repeatedly washing shrimp shells with running water, and drying; then crushing the mixture to obtain primary shrimp shell powder with the particle size of 0.125-0.15 mm;
(2) adding the shrimp shell primary powder and water into an ultrasonic stirrer, and carrying out ultrasonic treatment for 2.0 hours under the condition of 85KW power to uniformly mix the shrimp shell primary powder and the water to obtain slurry, wherein the mass ratio of the shrimp shell primary powder to the water is 1.2:1 during ultrasonic stirring and mixing; directly transferring the obtained slurry into a sand mill, performing sand milling treatment for 3.5h at a sand milling speed of 3000r/min, and drying to obtain shrimp shell powder;
(3) adding water and alkaline protease into the shrimp shell powder for hydrolysis for 1.0h, wherein the mass ratio of the shrimp shell powder to the water is 0.5:1, and the dosage of the alkaline protease is 1.0 IU/g; after the hydrolysis is finished, heating to 100 ℃, heating to inactivate the enzyme, and cooling to obtain protein hydrolysate;
(4) the protein concentration in the protein hydrolysate is measured by a biuret method, and the total amount of protein is calculated, so that the result shows that 279.33 mg of protein can be obtained by hydrolyzing the shrimp shell primary powder per gram after the treatment, and then the protein hydrolysate is spray-dried to obtain the protein powder.
Claims (8)
1. A method for efficiently recovering proteins in shrimp shells is characterized by comprising the following steps:
(1) repeatedly washing shrimp shells with running water, and drying; then crushing the mixture to obtain primary shrimp shell powder with the particle size of 0.125-0.15 mm;
(2) adding the shrimp shell primary powder and water into an ultrasonic stirrer, and uniformly mixing to obtain slurry; adding the slurry into a sand mill for sand milling treatment, and drying after sand milling to obtain shrimp shell powder;
(3) adding water and alkaline protease into the shrimp shell powder for hydrolysis, heating to 100 ℃ after hydrolysis, heating to inactivate enzyme, and cooling to obtain protein hydrolysate;
(4) and spray drying the obtained protein hydrolysate to obtain the protein powder.
2. The method for efficiently recovering the protein in the shrimp shells according to claim 1, wherein in the step (2), the mass ratio of the shrimp shell primary powder to the water is 0.1-1.5: 1.
3. The method for efficiently recovering the protein in the shrimp shells according to claim 1, wherein in the step (2), the mass ratio of the shrimp shell primary powder to the water is 0.8-1.2: 1.
4. The method for efficiently recovering the protein in the shrimp shells according to claim 1, wherein in the step (2), the ultrasonic power is 20-150 KW, and the ultrasonic time is 0.5-3 h.
5. The method for efficiently recovering proteins in shrimp shells according to claim 1, wherein in the step (2), the sand mill is operated at a constant speed, the speed of the sand mill is 1000-3500r/min, and the time of the sand mill is 1-5 h.
6. The method for efficiently recovering proteins from shrimp shells as claimed in claim 1, wherein in step (2), the sand mill is operated at a constant speed, the speed of the sand mill is 1500-.
7. The method for efficiently recovering proteins from shrimp shells according to claim 1, wherein in the step (3), the mass ratio of the shrimp shell powder to water is 0.5:1, the amount of alkaline protease is 0.5-1.5IU/g, and the hydrolysis time is 0.5-1.5 h.
8. The method for efficiently recovering proteins from shrimp shells according to claim 1, wherein in the step (3), the amount of the alkaline protease is 0.8-1.2IU/g, and the hydrolysis time is 1.0 h.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111665732.9A CN114410719A (en) | 2021-12-30 | 2021-12-30 | Method for efficiently recovering proteins in shrimp shells |
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| CN202111665732.9A CN114410719A (en) | 2021-12-30 | 2021-12-30 | Method for efficiently recovering proteins in shrimp shells |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676623A (en) * | 2012-05-23 | 2012-09-19 | 浙江大学 | Method for preparing antioxidant peptide from shrimp shell |
| CN103172763A (en) * | 2013-03-18 | 2013-06-26 | 武汉工业学院 | Coordinated process extraction method of biological active substances in crayfish by-products |
| CN105648003A (en) * | 2015-12-29 | 2016-06-08 | 广西钦州市绿源天然食品加工有限公司 | Method for recovering protein in shrimp heads and shrimp shells by enzyme hydrolysis method |
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2021
- 2021-12-30 CN CN202111665732.9A patent/CN114410719A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676623A (en) * | 2012-05-23 | 2012-09-19 | 浙江大学 | Method for preparing antioxidant peptide from shrimp shell |
| CN103172763A (en) * | 2013-03-18 | 2013-06-26 | 武汉工业学院 | Coordinated process extraction method of biological active substances in crayfish by-products |
| CN105648003A (en) * | 2015-12-29 | 2016-06-08 | 广西钦州市绿源天然食品加工有限公司 | Method for recovering protein in shrimp heads and shrimp shells by enzyme hydrolysis method |
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Inventor after: Ding Zhenzhong Inventor after: Shi Jinsong Inventor after: Gong Jinsong Inventor after: Li Ruiyi Inventor after: Qiu Yibin Inventor after: Guan Pinghai Inventor before: Ding Zhenzhong Inventor before: Shi Jinsong Inventor before: Deng Hongbing Inventor before: Sun Jian Inventor before: Gong Jinsong Inventor before: Li Ruiyi Inventor before: Qiu Yibin Inventor before: Guan Pinghai |
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