CN114391651A - Special sports nutritional compound deer raw material food and preparation method thereof - Google Patents
Special sports nutritional compound deer raw material food and preparation method thereof Download PDFInfo
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- CN114391651A CN114391651A CN202111413584.1A CN202111413584A CN114391651A CN 114391651 A CN114391651 A CN 114391651A CN 202111413584 A CN202111413584 A CN 202111413584A CN 114391651 A CN114391651 A CN 114391651A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/15—Vitamins
- A23L33/155—Vitamins A or D
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to the technical field of food, in particular to sports special nutrition compound deer raw material food and a preparation method thereof, wherein the food is prepared from the following raw materials in parts by weight, 10-30 parts of deer blood microcapsule powder; 10-25 parts of deer sinew polypeptide powder; 10-20 parts of deer bone microcapsule powder; 10-20 parts of deer tail polypeptide powder; 10-20 parts of deer penis polypeptide powder; 5-10 parts of ginseng peptide; 3-10 parts of rice bran fatty alkanol; 5-10 parts of euglena microcapsule powder; 2-4 parts of sodium hyaluronate; 1-2 parts of phosphatidylserine; 0.1-0.2 parts of vitamin D. By the synergistic effect of deer blood microcapsule powder, deer sinew, deer tail, deer penis polypeptide powder, deer bone microcapsule powder, ginseng peptide, rice bran fatty alkanol, euglena microcapsule powder, sodium hyaluronate, phosphatidylserine and vitamin D, after the food disclosed by the invention is eaten, nutrients for enhancing tendon and bone health can be supplemented for athletes, tendon strain and bone injury in training and competition of athletes can be prevented, and the strengthening and rehabilitation of joint and bone can be facilitated.
Description
Technical Field
The invention relates to the technical field of food, in particular to sports special nutritional compound deer raw material food and a preparation method thereof.
Background
In order to obtain excellent performances in a competition, the training intensity of athletes is continuously increased, and the body of the athletes is seriously injured by high-intensity training and competition, so that the normal performance of the athletes is influenced, and the development of the athletes is restricted. The injuries and diseases frequently occurring in the training and the competition of athletes are muscle strain, tendon strain, bone injury and the like, and the injuries and diseases are mainly caused by physical fatigue caused by a large amount of training.
The most important means for preventing tendon strain and bone injury of athletes in training and competition is to supplement nutrients for improving tendon and bone health for athletes every day, especially for teenagers. The stronger the tendon and the stronger the bone of the athlete, the less traumatic injuries such as tendon strain and fracture are likely to occur during the competition.
Meanwhile, along with the popularization of the national fitness, people who like high-strength sports such as marathon are rapidly increased, joint skeletal sports strain is also in high incidence, and the functional food which is beneficial to strengthening and rehabilitation of joint skeletons has a great market prospect.
Therefore, there is a need to develop a food for enhancing tendon and bone health, providing sufficient nutrition to athletes and people who are fond of sports, and preventing trauma during sports.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a sports special nutritional compound deer raw material food which has the advantages of promoting the joint bones to be strong and recovered.
The second purpose of the invention is to provide a preparation method of the sports special nutritional compound deer raw material food, which has the advantages of simple and rapid operation and capability of ensuring the effects of strengthening tendons and bones of the food.
In order to achieve the first object, the invention provides the following technical scheme:
a sports special nutritional compound deer raw material food is prepared from the following raw materials in parts by weight, 10-30 parts of deer blood microcapsule powder; 10-25 parts of deer sinew polypeptide powder; 10-20 parts of deer bone microcapsule powder; 10-20 parts of deer tail polypeptide powder; 10-20 parts of deer penis polypeptide powder; 5-10 parts of ginseng peptide; 3-10 parts of rice bran fatty alkanol; 5-10 parts of euglena microcapsule powder; 2-4 parts of sodium hyaluronate; 1-2 parts of phosphatidylserine; 0.1-0.2 parts of vitamin D.
By adopting the technical scheme, after athletes and people who are fond of sports eat the food, firstly, in the deer raw material, the protein which is the main component in the deer blood microcapsule powder can improve the content of hemoglobin in blood, so as to improve the endurance of human bodies, other components such as various lipids, free fatty acids, sterols and phospholipid substances can obviously improve the anti-fatigue capability of human bodies, so as to prolong the market of sports such as weight-bearing swimming, in addition, the deer blood microcapsule powder can also improve the immunity and wound healing rate of human bodies, and has obvious effects on treating diseases such as weakness, fracture, hemoptysis, and the like, and the deer tendon, deer tail and deer penis polypeptide powder has the effects of nourishing blood, dredging collaterals, strengthening tendons and bones, producing sperm and benefiting marrow, mainly treating strain, continuing injury, strengthening muscles and bones, tonifying yang and qi, and has the effects on long-suffered from rheumatism, arthralgia, lumbar spinal pain, muscle and muscle fatigue or weak weakness, The deer bone microcapsule powder contains a large amount of protein, a plurality of trace elements, a large amount of calcium phosphate and a few of trace element phosphorus, so that the food has the effects of strengthening bones and marrow, and has good prevention and repair effects on tendon strain, fracture and bone fracture which are easy to occur in training and competition; the sodium hyaluronate is a new food raw material, and shows various important physiological functions in organisms by using unique molecular structure and physicochemical properties, such as joint lubrication, adjustment of permeability of blood vessel walls, adjustment of diffusion and operation of protein and water electrolytes, promotion of wound healing and the like. Secondly, on the basis of synergistically protecting the health of bones and muscle tendons by deer blood microcapsule powder, deer sinew, deer tail, deer penis polypeptide powder, deer bone microcapsule powder and sodium hyaluronate, the euglena is rich in omega-3 polyunsaturated fatty acid (omega-3), the omega-3 has the functions of improving memory, reaction capacity and learning capacity and is a key substance for normal activity of brain cells, and meanwhile, phosphatidylserine is a core component of the brain cells and nervous tissues and has the effects of improving the functions of memory, learning capacity and reaction capacity; thirdly, the ginseng peptide has the functions of reinforcing primordial qi, recovering pulse, relieving depletion, tonifying spleen, benefiting lung, promoting production of body fluid and soothing nerves, can improve the immunity function, the anti-fatigue function, the memory and learning ability function, the cardiac function, the myocardial contraction force and the cardiac output, has the protection and promotion function on the bone marrow hematopoiesis function and the anti-stress function, and each of the six functions is very important for athletes; finally, vitamin D is a key factor for promoting calcium absorption, Chinese people (including athletes) have no habit and condition of sunbathing and have no knowledge of supplementing vitamin D from food, so that the calcium deficiency phenomenon of Chinese people (including athletes) generally exists and affects the health of bones, and the recent research in the United states proves that the effect of vitamin D on the brain is far better than the effect on the bones, and the proper supplement of vitamin D is helpful for regulating enzymes in the brain and cerebral marrow fluid, and the enzymes are relevant to manufacturing neurotransmitters; in conclusion, by the synergistic effect of the deer blood microcapsule powder, the deer sinew, the deer tail, the deer penis polypeptide powder, the deer bone microcapsule powder, the ginseng peptide, the rice bran fatty alkanol, the euglena microcapsule powder, the sodium hyaluronate, the phosphatidylserine and the vitamin D, after eating the food disclosed by the invention, the food can supplement nutrients for enhancing the health of tendons and bones for athletes, prevent the tendons from being pulled and the bones from being damaged in training and competition of the athletes, and is beneficial to strengthening and recovering the bones and the joints.
In order to achieve the second object, the invention provides the following technical scheme:
a preparation method of sports special nutrition compound deer raw material food comprises the following steps of S1 raw material pretreatment (a) extracting euglena, mixing and shearing the extracted euglena oil and composite wall material solution, and then sequentially homogenizing and spray drying to obtain euglena microcapsule powder; (b) pulverizing ligamentum Cervi, cauda Cervi and penis et testis Cervi, colloid milling, performing enzymolysis, sterilizing and solid-liquid separation, vacuum concentrating and spray drying the separated ligamentum Cervi, cauda Cervi and penis et testis Cervi polypeptide liquid respectively to obtain polypeptide powder; (c) respectively carrying out low-temperature freeze drying, coarse crushing and nano crushing on the deer blood and the deer bone to obtain deer blood microcapsule powder and deer bone microcapsule powder; s2 preparing materials containing ginseng peptide, rice bran fatty alkanol, phosphatidylserine, vitamin D, and the deer blood microcapsule powder, deer tendon, deer tail, deer penis polypeptide powder, deer bone microcapsule powder and euglena microcapsule powder obtained from S1; s3 embedding the raw material of S2 by microcapsule embedding method to obtain the food.
By adopting the technical scheme, in the pre-treatment step, important raw materials such as euglena, deer sinew, deer tail, deer penis, deer bone, deer blood and the like are processed into powdery substances with smaller particle size mainly by means of extraction, crushing and the like, so that the raw materials are convenient to store, the utilization rate of nutrient components is improved, the stability of the raw materials is improved, the mutual interference among various components is prevented, and the raw materials are easier to absorb and utilize by a human body; the loss of flavor substances in the food raw materials is inhibited by a microcapsule embedding method, so that the effects of protecting nutrient components and controlling release can be achieved; the food obtained by the method not only has simple and rapid operation in the preparation process, but also can ensure the effect of strengthening tendons and bones of the food.
Further, in the step S1, the process a includes (1) using supercritical CO2Extracting euglena at a low temperature of 35-45 ℃, under the extraction pressure of 30-40 MPa for 110-130 min to obtain euglena oil; (2) firstly, the mass ratio is (1.5-2.5): 1, mixing the corn starch and maltodextrin to obtain a composite wall material, and dissolving the composite wall material in water to obtain a composite wall material solution with the mass concentration of 30-45%; (3) the composite wall material solution is used as a water phase, the Euglena oil is used as an oil phase, and the mass ratio of the water phase to the oil phase is (3-5): 1, mixing and shearing to obtain a treatment solution; (4) homogenizing the treated liquid, and spray drying the homogenized treated liquid to obtain Euglena microcapsule powder. The euglena microcapsule powder is used as a core material component of a microcapsule embedding method, can directly influence the slow release rate and the stability of the core material, and the corn starch and the maltodextrin are used as composite wall materials to emulsify the euglena oil, so that the flavor of the core material can be improved, and the storage stability of the core material is ensured.
Specifically, in the shearing treatment, the shearing speed is 1500-2500 r/min, the shearing time is 20-40 min, and the shearing temperature is 30-45 ℃.
Specifically, in the homogenizing treatment, the homogenizing pressure is 30-40 MPa, the homogenizing temperature is 40-50 ℃, and the homogenizing times are 2-3.
Specifically, in the spray drying treatment, the inlet air temperature of spray drying is 50-170 ℃, the outlet air temperature is 70-90 ℃, the frequency of a high-pressure pump is 10-30 Hz, and the atomization rotating speed is 25-35 r/min.
Further, in the step S1, the step b includes (1) mixing the deer tendon, the deer tail and the deer penis with water, and then performing fine crushing and colloid milling to obtain a deer tendon, deer tail and deer penis suspension with a mass concentration of 10-20%; (2) respectively carrying out enzymolysis on the deer sinew, deer tail and deer penis suspension by adopting a two-step enzymolysis method, and respectively carrying out two-step enzymolysis by using alkaline protease and neutral protease at the enzymolysis temperature of 50-55 ℃ to obtain deer sinew, deer tail and deer penis enzymolysis liquid; (3) carrying out sterilization treatment on the deer sinew, deer tail and deer penis enzymatic hydrolysate by UHT (ultra high temperature) at the temperature of 138-143 ℃ for 7-9 s, and carrying out solid-liquid separation treatment to obtain deer sinew, deer tail and deer penis polypeptide liquid; (4) sequentially carrying out vacuum concentration and spray drying on the deer sinew, the deer tail and the deer penis polypeptide liquid to obtain the deer sinew, the deer tail and the deer penis polypeptide powder. The ligamentum cervi, the cauda cervi and the penis cervi polypeptide powder are used as capsule wall components of a microcapsule embedding method, and by a two-step enzymolysis treatment mode, not only are harmful components of a traditional capsule shell avoided, but also various advantages of slow release absorption, nutrient component volatilization prevention and the like can be achieved.
Further, in the step S1, the step c comprises the steps of (1) respectively carrying out low-temperature freeze drying treatment on the deer blood and the deer bone, wherein the freeze drying temperature is-75 to-80 ℃, and the freeze drying time is 12 to 15 hours, so as to obtain freeze-dried deer blood and freeze-dried deer bone; (2) respectively coarsely pulverizing the freeze-dried deer blood and the freeze-dried deer bone in an FSJ-320 type pulverizer at the rotating speed of 3000-4000 r/min to obtain deer blood coarse powder and deer bone coarse powder; (3) respectively carrying out nano-crushing on the deer blood coarse powder and the deer bone coarse powder in an KQW type nano-crusher, wherein the crusher power is 4500-5500 kw and the rotation speed is 9500-10500 r/min, and obtaining the deer blood micro-capsule powder and the deer bone micro-capsule powder. The mode of coarse grinding and nano grinding is adopted, so that the particle sizes of the deer blood microcapsule powder and the deer bone microcapsule powder are small and uniform while high efficiency is achieved.
Further, the step S3 includes (1) mixing euglena microcapsule powder, deer blood microcapsule powder, deer bone microcapsule powder, ginseng peptide, rice bran fatty alkanol, phosphatidylserine, vitamin D and excipient, and stirring for 20-40 min to obtain mixed powder; (2) mixing deer sinew, deer tail, deer penis polypeptide powder, isomaltooligosaccharide syrup, sodium caseinate, sodium hyaluronate and flaxseed gum, and stirring for 20-40 min to obtain an embedding solution; (3) and introducing the mixed powder into a fluidized bed of clean hot air to be in a boiling state, introducing the embedding liquid into a pressure type nozzle for atomization, and treating the mixed powder and the atomized embedding liquid by adopting a microcapsule embedding method to obtain the food. The basic principle of the step (3) is that embedding liquid formed by a pressure type nozzle is atomized and contacts and bonds with mixed powder to form a film, the film is dried by hot air, the film is repeatedly embedded and polymerized to form microcapsule powder of 40-80-mesh round particles, and then the microcapsule powder is prepared into different formulations of foods such as tablets, powder, granules, nutrition bars, liquid drinks and the like.
Specifically, sterile air pressure of the fluidized bed is 2-3 kg/cm, flow rate of fluidized air is 11-13 m for thin film fruit cultivation/h, hot air temperature is 40-60 ℃, and flow rate of spray head air is 2-3 m for thin film fruit cultivation/h.
In conclusion, the beneficial technical effects of the invention are as follows:
1. by the synergistic effect of deer blood microcapsule powder, deer sinew, deer tail, deer penis polypeptide powder, deer bone microcapsule powder, ginseng peptide, rice bran fatty alkanol, euglena microcapsule powder, sodium hyaluronate, phosphatidylserine and vitamin D, after the food disclosed by the invention is eaten, nutrients for enhancing tendon and bone health can be supplemented for athletes, tendon strain and bone injury in training and competition of athletes can be prevented, and the strengthening and rehabilitation of joint bones are facilitated;
2. the method of the invention not only has simple and rapid operation in the preparation process, but also can ensure the effect of strengthening tendons and bones of the food.
Detailed Description
In order to make the technical means, the creation features, the achievement purposes and the functions of the invention clearer and easier to understand, the invention is further described in the following with the specific embodiments.
Example 1: a preparation method of sports special nutritional compound deer raw material food comprises the following steps,
s1 raw material pretreatment
(a) Extracting euglena, mixing and shearing the extracted euglena oil and the composite wall material solution, and then sequentially homogenizing and spray drying to obtain euglena microcapsule powder;
specifically, the process a comprises the following steps,
(1) extracting Euglena at low temperature with supercritical CO2 at 35 deg.C under 40MPa for 125min to obtain Euglena oil;
(2) firstly, the mass ratio is 1.5: 1, mixing the corn starch and maltodextrin to obtain a composite wall material, and dissolving the composite wall material in water to obtain a composite wall material solution with the mass concentration of 30%;
(3) taking the composite wall material solution as a water phase and the euglena oil as an oil phase, wherein the mass ratio of the water phase to the oil phase is 4: 1, mixing and shearing at the shearing speed of 1500r/min for 30min and at the shearing temperature of 30 ℃ to obtain a treatment solution;
(4) homogenizing the treated liquid at a homogenizing pressure of 30MPa, a homogenizing temperature of 42 deg.C and homogenizing times of 2 times, spray drying at an inlet air temperature of 50 deg.C, an outlet air temperature of 80 deg.C, a high-pressure pump frequency of 25Hz, and an atomizing speed of 30r/min to obtain Euglena microcapsule powder.
(b) Pulverizing ligamentum Cervi, cauda Cervi and penis et testis Cervi, colloid milling, performing enzymolysis, sterilizing and solid-liquid separation, vacuum concentrating and spray drying the separated ligamentum Cervi, cauda Cervi and penis et testis Cervi polypeptide liquid respectively to obtain polypeptide powder;
specifically, the process b comprises the following steps,
(1) mixing ligamentum Cervi, cauda Cervi, penis et testis Cervi with water, pulverizing, and colloid milling to obtain 10% injectable suspension;
(2) respectively carrying out enzymolysis on the deer sinew, deer tail and deer penis suspension by adopting a two-step enzymolysis method, and respectively carrying out two-step enzymolysis by using alkaline protease and neutral protease at the enzymolysis temperature of 55 ℃ to obtain deer sinew, deer tail and deer penis enzymolysis liquid;
(3) sterilizing the ligamentum Cervi, cauda Cervi and penis et testis Cervi enzymolysis solution by UHT at 141 deg.C for 8s, and performing solid-liquid separation to obtain ligamentum Cervi, cauda Cervi and penis et testis Cervi polypeptide solution;
(4) sequentially carrying out vacuum concentration and spray drying treatment on the ligamentum cervi, the cauda cervi and the penis cervi polypeptide liquid, wherein the air inlet temperature of the spray drying is 50 ℃, the air outlet temperature is 80 ℃, the frequency of a high-pressure pump is 25Hz, and the atomization rotating speed is 30r/min, so as to obtain the ligamentum cervi, the cauda cervi and the penis cervi polypeptide powder.
(c) Respectively carrying out low-temperature freeze drying, coarse crushing and nano crushing on the deer blood and the deer bone to obtain deer blood microcapsule powder and deer bone microcapsule powder;
specifically, the process c comprises the following steps,
(1) freeze drying sanguis Cervi and Os Cervi at low temperature of-75 deg.C for 13 hr to obtain lyophilized sanguis Cervi and lyophilized Os Cervi;
(2) respectively coarsely pulverizing lyophilized sanguis Cervi and lyophilized Os Cervi in FSJ-320 type pulverizer at 3000r/min to obtain sanguis Cervi coarse powder and Os Cervi coarse powder;
(3) respectively carrying out nano-grinding on the deer blood coarse powder and the deer bone coarse powder in an KQW type nano-grinder, wherein the grinder power is 4500kw and the rotation speed is 9500r/min, and obtaining the deer blood microcapsule powder and the deer bone microcapsule powder.
S2 preparing materials including ginseng peptide, rice bran fatty alkanol, sodium hyaluronate, phosphatidylserine, vitamin D, and deer blood microcapsule powder, deer tendon, deer tail, deer penis polypeptide powder, deer bone microcapsule powder and euglena microcapsule powder obtained in S1;
specifically, the deer blood microcapsule is prepared from the following raw materials in parts by weight, 10 parts of deer blood microcapsule powder; 5 parts of deer sinew, deer tail and deer penis polypeptide powder; 30 parts of deer bone microcapsule powder; 8 parts of ginseng peptide; 5 parts of rice bran fatty alkanol; 7 parts of euglena microcapsule powder; 2 parts of phosphatidylserine; 0.1 part of vitamin D.
S3 the raw material of S2 is embedded by microcapsule embedding method to obtain the food.
Specifically, S3 includes, for example,
(1) mixing Euglena microcapsule powder, sanguis Cervi microcapsule powder, Os Cervi microcapsule powder, Ginseng radix peptide, testa oryzae fatty alkanol, phosphatidylserine, vitamin D and excipient, and stirring for 30min to obtain mixed powder;
(2) mixing ligamentum Cervi polypeptide powder, isomaltooligosaccharide syrup, sodium caseinate and flaxseed gum, and stirring for 30min to obtain embedding solution;
(3) introducing the mixed powder into a clean hot air fluidized bed and boiling, wherein the sterile air pressure of the fluidized bed is 2kg/cm, the flow rate of the fluidized air is 12m top year/h, the temperature of the hot air is 50 ℃, and the flow rate of the spray head air is 3m top year/h; meanwhile, introducing the embedding liquid into a pressure type nozzle for atomization, treating the mixed powder and the atomized embedding liquid by a microcapsule embedding method, contacting and bonding the mixed powder and the atomized embedding liquid into a film, drying the film by hot air, embedding and recombining for multiple times to form microcapsule powder of 40-80-mesh round particles, and preparing the microcapsule powder into different formulations of foods such as tablets, powder, granules, nutrition bars, liquid drinks and the like.
Example 2:
a preparation method of sports special nutritional compound deer raw material food comprises the following steps,
s1 raw material pretreatment
(a) Extracting euglena, mixing and shearing the extracted euglena oil and the composite wall material solution, and then sequentially homogenizing and spray drying to obtain euglena microcapsule powder;
specifically, the process a comprises the following steps,
(1) extracting Euglena at low temperature with supercritical CO2 at 38 deg.C under 37MPa for 120min to obtain Euglena oil;
(2) firstly, the mass ratio is 1.3: 1, mixing the corn starch and maltodextrin to obtain a composite wall material, and dissolving the composite wall material in water to obtain a composite wall material solution with the mass concentration of 35%;
(3) taking the composite wall material solution as a water phase and the euglena oil as an oil phase, wherein the mass ratio of the water phase to the oil phase is 3: 1, mixing and shearing at the shearing speed of 2000r/min for 40min and at the shearing temperature of 35 ℃ to obtain a treatment solution;
(4) homogenizing the treated liquid at a homogenizing pressure of 35MPa, a homogenizing temperature of 40 deg.C and homogenizing times of 2 times, spray drying at air inlet temperature of 80 deg.C and air outlet temperature of 70 deg.C, high-pressure pump frequency of 10Hz, and atomizing speed of 35r/min to obtain Euglena microcapsule powder.
(b) Pulverizing ligamentum Cervi, colloid milling, performing enzymolysis, sterilizing and solid-liquid separation, and vacuum concentrating and spray drying the separated ligamentum Cervi polypeptide liquid to obtain Os Cervi microcapsule powder;
specifically, the process b comprises the following steps,
(1) mixing ligamentum Cervi, cauda Cervi, penis et testis Cervi with water, pulverizing, and colloid milling to obtain 12% injectable suspension;
(2) respectively carrying out enzymolysis on the deer sinew, deer tail and deer penis suspension by adopting a two-step enzymolysis method, and respectively carrying out two-step enzymolysis by using alkaline protease and neutral protease at the enzymolysis temperature of 54 ℃ to obtain deer sinew, deer tail and deer penis enzymolysis liquid;
(3) sterilizing the ligamentum Cervi, cauda Cervi and penis et testis Cervi enzymolysis solution by UHT at 138 deg.C for 8s, and performing solid-liquid separation to obtain ligamentum Cervi, cauda Cervi and penis et testis Cervi polypeptide solution;
(4) sequentially carrying out vacuum concentration and spray drying treatment on the deer sinew, the deer tail and the deer penis polypeptide liquid, wherein the air inlet temperature of the spray drying is 80 ℃, the air outlet temperature is 70 ℃, the frequency of a high-pressure pump is 10Hz, and the atomization rotating speed is 35r/min, so as to obtain the deer sinew, deer tail and deer penis polypeptide powder.
(c) Respectively carrying out low-temperature freeze drying, coarse crushing and nano crushing on the deer blood and the deer bone to obtain deer blood microcapsule powder and deer bone microcapsule powder;
specifically, the process c comprises the following steps,
(1) freeze drying sanguis Cervi and Os Cervi at low temperature of-78 deg.C for 12 hr to obtain lyophilized sanguis Cervi and lyophilized Os Cervi;
(2) respectively coarsely pulverizing lyophilized sanguis Cervi and lyophilized Os Cervi in FSJ-320 type pulverizer at 3500r/min to obtain sanguis Cervi coarse powder and Os Cervi coarse powder;
(3) respectively carrying out nano-grinding on the deer blood coarse powder and the deer bone coarse powder in an KQW type nano-grinder, wherein the grinder power is 5500kw, and the rotation speed is 10000r/min, so as to obtain the deer blood micro-capsule powder and the deer bone micro-capsule powder.
S2 preparing materials containing ginseng peptide, rice bran fatty alkanol, phosphatidylserine, vitamin D, and the deer blood microcapsule powder, deer tendon, deer tail, deer penis polypeptide powder, deer bone microcapsule powder and euglena microcapsule powder obtained from S1;
specifically, the deer blood microcapsule is prepared from the following raw materials in parts by weight, 20 parts of deer blood microcapsule powder; 12 parts of deer sinew, deer tail and deer penis polypeptide powder; 40 parts of deer bone microcapsule powder; 8 parts of ginseng peptide; 3 parts of rice bran fatty alkanol; 5 parts of euglena microcapsule powder; 2 parts of sodium hyaluronate; 2 parts of phosphatidylserine; 0.1 part of vitamin D.
S3 the raw material of S2 is embedded by microcapsule embedding method to obtain the food.
Specifically, S3 includes, for example,
(1) mixing Euglena microcapsule powder, sanguis Cervi microcapsule powder, Os Cervi microcapsule powder, Ginseng radix peptide, testa oryzae fatty alkanol, phosphatidylserine, vitamin D and excipient, and stirring for 20min to obtain mixed powder;
(2) mixing ligamentum Cervi, cauda Cervi, penis et testis Cervi polypeptide powder, isomaltooligosaccharide syrup, sodium caseinate, sodium hyaluronate and flaxseed gum, and stirring for 20min to obtain embedding solution;
(3) introducing the mixed powder into a clean hot air fluidized bed and boiling, wherein the sterile air pressure of the fluidized bed is 2kg/cm, the flow rate of the fluidized air is 12m top year/h, the temperature of the hot air is 40 ℃, and the flow rate of the spray head air is 3m top year/h; meanwhile, introducing the embedding liquid into a pressure type nozzle for atomization, treating the mixed powder and the atomized embedding liquid by a microcapsule embedding method, contacting and bonding the mixed powder and the atomized embedding liquid into a film, drying the film by hot air, embedding and recombining for multiple times to form microcapsule powder of 40-80-mesh round particles, and preparing the microcapsule powder into different formulations of foods such as tablets, powder, granules, nutrition bars, liquid drinks and the like.
Example 3:
a preparation method of sports special nutritional compound deer raw material food comprises the following steps,
s1 raw material pretreatment
(a) Extracting euglena, mixing and shearing the extracted euglena oil and the composite wall material solution, and then sequentially homogenizing and spray drying to obtain euglena microcapsule powder;
specifically, the process a comprises the following steps,
(1) extracting Euglena at low temperature with supercritical CO2 at 40 deg.C under 35MPa for 110min to obtain Euglena oil;
(2) firstly, the mass ratio is 1.9: 1, mixing the corn starch and maltodextrin to obtain a composite wall material, and dissolving the composite wall material in water to obtain a composite wall material solution with the mass concentration of 40%;
(3) taking the composite wall material solution as a water phase and the euglena oil as an oil phase, wherein the mass ratio of the water phase to the oil phase is 3: 1, mixing and shearing at the shearing speed of 2500r/min for 20min and at the shearing temperature of 40 ℃ to obtain a treatment solution;
(4) homogenizing the treated liquid at a homogenizing pressure of 34MPa, a homogenizing temperature of 50 deg.C and homogenizing times of 2 times, spray drying at an inlet air temperature of 60 deg.C and an outlet air temperature of 75 deg.C, a high-pressure pump at a frequency of 20Hz, and an atomizing speed of 25r/min to obtain Euglena microcapsule powder.
(b) Pulverizing ligamentum Cervi, cauda Cervi and penis et testis Cervi, colloid milling, performing enzymolysis, sterilizing and solid-liquid separation, vacuum concentrating and spray drying the separated ligamentum Cervi, cauda Cervi and penis et testis Cervi polypeptide liquid respectively to obtain ligamentum Cervi, cauda Cervi and penis et testis Cervi polypeptide powder;
specifically, the process b comprises the following steps,
(1) mixing ligamentum Cervi, cauda Cervi, penis et testis Cervi with water, pulverizing, and colloid milling to obtain 15% injectable suspension;
(2) respectively carrying out enzymolysis on the deer sinew, deer tail and deer penis suspension by adopting a two-step enzymolysis method, and respectively carrying out two-step enzymolysis by using alkaline protease and neutral protease at the enzymolysis temperature of 52 ℃ to obtain deer sinew, deer tail and deer penis enzymolysis liquid;
(3) sterilizing the ligamentum Cervi, cauda Cervi and penis et testis Cervi enzymolysis solution by UHT at 143 deg.C for 8s, and performing solid-liquid separation to obtain ligamentum Cervi, cauda Cervi and penis et testis Cervi polypeptide solution;
(4) sequentially carrying out vacuum concentration and spray drying treatment on the ligamentum cervi, the cauda cervi and the penis cervi polypeptide liquid, wherein the air inlet temperature of the spray drying is 60 ℃, the air outlet temperature is 75 ℃, the frequency of a high-pressure pump is 20Hz, and the atomization rotating speed is 25r/min, so as to obtain the ligamentum cervi, the cauda cervi and the penis cervi polypeptide powder.
(c) Respectively carrying out low-temperature freeze drying, coarse crushing and nano crushing on the deer blood and the deer bone to obtain deer blood microcapsule powder and deer bone microcapsule powder;
specifically, the process c comprises the following steps,
(1) freeze drying sanguis Cervi and Os Cervi at low temperature of-80 deg.C for 14 hr to obtain lyophilized sanguis Cervi and lyophilized Os Cervi;
(2) respectively coarsely pulverizing lyophilized sanguis Cervi and lyophilized Os Cervi in FSJ-320 type pulverizer at 3600r/min to obtain sanguis Cervi coarse powder and Os Cervi coarse powder;
(3) respectively carrying out nano-grinding on the deer blood coarse powder and the deer bone coarse powder in an KQW type nano-grinder, wherein the grinder power is 4900kw and the rotation speed is 10500r/min, and obtaining the deer blood microcapsule powder and the deer bone microcapsule powder.
S2 preparing materials containing ginseng peptide, rice bran fatty alkanol, phosphatidylserine, vitamin D, and the deer blood microcapsule powder, deer tendon, deer tail, deer penis polypeptide powder, deer bone microcapsule powder and euglena microcapsule powder obtained from S1;
specifically, the deer blood microcapsule is prepared from 30 parts by weight of deer blood microcapsule powder; 15 parts of deer sinew, deer tail and deer penis polypeptide powder; 20 parts of deer bone microcapsule powder; 5 parts of ginseng peptide; 6 parts of rice bran fatty alkanol; 10 parts of euglena microcapsule powder; 2 parts of sodium hyaluronate and 1 part of phosphatidylserine; 0.1 part of vitamin D.
S3 the raw material of S2 is embedded by microcapsule embedding method to obtain the food.
Specifically, S3 includes, for example,
(1) mixing Euglena microcapsule powder, sanguis Cervi microcapsule powder, Os Cervi microcapsule powder, Ginseng radix peptide, testa oryzae fatty alkanol, phosphatidylserine, vitamin D and excipient, and stirring for 40min to obtain mixed powder;
(2) mixing ligamentum Cervi, cauda Cervi, penis et testis Cervi polypeptide powder, isomaltooligosaccharide syrup, sodium caseinate, sodium hyaluronate and flaxseed gum, and stirring for 40min to obtain embedding solution;
(3) introducing the mixed powder into a clean hot air fluidized bed and boiling, wherein the sterile air pressure of the fluidized bed is 3kg/cm, the flow rate of the fluidized air is 11m top year/h, the temperature of the hot air is 60 ℃, and the flow rate of the spray head air is 3m top year/h; meanwhile, introducing the embedding liquid into a pressure type nozzle for atomization, treating the mixed powder and the atomized embedding liquid by a microcapsule embedding method, contacting and bonding the mixed powder and the atomized embedding liquid into a film, drying the film by hot air, embedding and recombining for multiple times to form microcapsule powder of 40-80-mesh round particles, and preparing the microcapsule powder into different formulations of foods such as tablets, powder, granules, nutrition bars, liquid drinks and the like.
Example 4:
a preparation method of sports special nutritional compound deer raw material food comprises the following steps,
s1 raw material pretreatment
(a) Extracting euglena, mixing and shearing the extracted euglena oil and the composite wall material solution, and then sequentially homogenizing and spray drying to obtain euglena microcapsule powder;
specifically, the process a comprises the following steps,
(1) extracting Euglena at low temperature with supercritical CO2 at 42 deg.C under 32MPa for 115min to obtain Euglena oil;
(2) firstly, the mass ratio is 2.2: 1, mixing the corn starch and maltodextrin to obtain a composite wall material, and dissolving the composite wall material in water to obtain a composite wall material solution with the mass concentration of 45%;
(3) taking the composite wall material solution as a water phase and the euglena oil as an oil phase, wherein the mass ratio of the water phase to the oil phase is 5: 1, mixing and shearing at 1750r/min for 35min at 45 ℃ to obtain a treatment solution;
(4) homogenizing the treated liquid at a homogenizing pressure of 38MPa, a homogenizing temperature of 48 deg.C and homogenizing times of 3 times, spray drying at a high-pressure pump frequency of 30Hz and an atomizing speed of 27r/min to obtain Euglena microcapsule powder, wherein the inlet air temperature of spray drying is 120 deg.C, the outlet air temperature is 90 deg.C.
(b) Pulverizing ligamentum Cervi, colloid milling, performing enzymolysis, sterilizing, and performing solid-liquid separation, and vacuum concentrating and spray drying the separated ligamentum Cervi, cauda Cervi and penis et testis Cervi polypeptide liquid to obtain ligamentum Cervi, cauda Cervi and penis et testis Cervi polypeptide powder;
specifically, the process b comprises the following steps,
(1) mixing ligamentum Cervi, cauda Cervi, penis et testis Cervi with water, pulverizing, and colloid milling to obtain 18% injectable suspension;
(2) respectively carrying out enzymolysis on the deer sinew, deer tail and deer penis suspension by adopting a two-step enzymolysis method, and respectively carrying out two-step enzymolysis by using alkaline protease and neutral protease at the enzymolysis temperature of 53 ℃ to obtain deer sinew, deer tail and deer penis enzymolysis liquid;
(3) sterilizing the ligamentum Cervi, cauda Cervi and penis et testis Cervi enzymolysis solution by UHT at 141 deg.C for 7s, and performing solid-liquid separation to obtain ligamentum Cervi, cauda Cervi and penis et testis Cervi polypeptide solution;
(4) sequentially carrying out vacuum concentration and spray drying treatment on the deer sinew, the deer tail and the deer penis polypeptide liquid, wherein the air inlet temperature of the spray drying is 120 ℃, the air outlet temperature is 90 ℃, the frequency of a high-pressure pump is 30Hz, and the atomization rotating speed is 27r/min, so as to obtain the deer sinew, deer tail and deer penis polypeptide powder.
(c) Respectively carrying out low-temperature freeze drying, coarse crushing and nano crushing on the deer blood and the deer bone to obtain deer blood microcapsule powder and deer bone microcapsule powder;
specifically, the process c comprises the following steps,
(1) freeze drying sanguis Cervi and Os Cervi at low temperature of-79 deg.C for 15 hr to obtain lyophilized sanguis Cervi and lyophilized Os Cervi;
(2) respectively coarsely pulverizing lyophilized sanguis Cervi and lyophilized Os Cervi in FSJ-320 type pulverizer at 4000r/min to obtain sanguis Cervi coarse powder and Os Cervi coarse powder;
(3) respectively carrying out nano-grinding on the deer blood coarse powder and the deer bone coarse powder in an KQW type nano-grinder, wherein the grinder power is 5300kw and the rotating speed is 10200r/min, so as to obtain the deer blood microcapsule powder and the deer bone microcapsule powder.
S2 preparing materials containing ginseng peptide, rice bran fatty alkanol, phosphatidylserine, vitamin D, and the deer blood microcapsule powder, deer tendon, deer tail, deer penis polypeptide powder, deer bone microcapsule powder and euglena microcapsule powder obtained from S1;
specifically, the deer blood microcapsule is prepared from the following raw materials in parts by weight, 15 parts of deer blood microcapsule powder; 10 parts of deer sinew, deer tail and deer penis polypeptide powder; 25 parts of deer bone microcapsule powder; 10 parts of ginseng peptide; 10 parts of rice bran fatty alkanol; 6 parts of euglena microcapsule powder; 2 parts of sodium hyaluronate; 1 part of phosphatidylserine; 0.1 part of vitamin D.
S3 the raw material of S2 is embedded by microcapsule embedding method to obtain the food.
Specifically, S3 includes, for example,
(1) mixing Euglena microcapsule powder, sanguis Cervi microcapsule powder, Os Cervi microcapsule powder, Ginseng radix peptide, testa oryzae fatty alkanol, phosphatidylserine, vitamin D and excipient, and stirring for 35min to obtain mixed powder;
(2) mixing ligamentum Cervi polypeptide powder, isomaltooligosaccharide syrup, sodium caseinate, sodium hyaluronate and flaxseed gum, and stirring for 35min to obtain embedding solution;
(3) introducing the mixed powder into a clean hot air fluidized bed and boiling, wherein the sterile air pressure of the fluidized bed is 3kg/cm, the flow rate of the fluidized air is 12m top year/h, the temperature of the hot air is 50 ℃, and the flow rate of the spray head air is 2m top year/h; meanwhile, introducing the embedding liquid into a pressure type nozzle for atomization, treating the mixed powder and the atomized embedding liquid by a microcapsule embedding method, contacting and bonding the mixed powder and the atomized embedding liquid into a film, drying the film by hot air, embedding and recombining for multiple times to form microcapsule powder of 40-80-mesh round particles, and preparing the microcapsule powder into different formulations of foods such as tablets, powder, granules, nutrition bars, liquid drinks and the like.
Example 5:
a preparation method of sports special nutritional compound deer raw material food comprises the following steps,
s1 raw material pretreatment
(a) Extracting euglena, mixing and shearing the extracted euglena oil and the composite wall material solution, and then sequentially homogenizing and spray drying to obtain euglena microcapsule powder;
specifically, the process a comprises the following steps,
(1) extracting Euglena at low temperature with supercritical CO2 at 45 deg.C under 30MPa for 130min to obtain Euglena oil;
(2) firstly, the mass ratio is 2.5: 1, mixing the corn starch and maltodextrin to obtain a composite wall material, and dissolving the composite wall material in water to obtain a composite wall material solution with the mass concentration of 35%;
(3) taking the composite wall material solution as a water phase and the euglena oil as an oil phase, wherein the mass ratio of the water phase to the oil phase is 5: 1, mixing and shearing at 2230r/min for 25min at 35 deg.C to obtain a treating liquid;
(4) homogenizing the treated liquid at 40MPa, 45 deg.C for 3 times, spray drying at air inlet temperature of 170 deg.C and air outlet temperature of 88 deg.C, high-pressure pump frequency of 15Hz, and atomizing speed of 32r/min to obtain Euglena microcapsule powder.
(b) Pulverizing ligamentum Cervi, cauda Cervi and penis et testis Cervi, colloid milling, performing enzymolysis, sterilizing and solid-liquid separation, vacuum concentrating and spray drying the separated ligamentum Cervi, cauda Cervi and penis et testis Cervi polypeptide liquid respectively to obtain ligamentum Cervi, cauda Cervi and penis et testis Cervi polypeptide powder;
specifically, the process b comprises the following steps,
(1) mixing ligamentum Cervi, cauda Cervi, penis et testis Cervi with water, pulverizing, and colloid milling to obtain 20% ligamentum Cervi, cauda Cervi, penis et testis Cervi suspension;
(2) respectively carrying out enzymolysis on the deer sinew, deer tail and deer penis suspension by adopting a two-step enzymolysis method, and respectively carrying out two-step enzymolysis by using alkaline protease and neutral protease at the enzymolysis temperature of 50 ℃ to obtain deer sinew, deer tail and deer penis enzymolysis liquid;
(3) sterilizing the ligamentum Cervi, cauda Cervi and penis et testis Cervi enzymolysis solution by UHT at 141 deg.C for 9s, and performing solid-liquid separation to obtain ligamentum Cervi, cauda Cervi and penis et testis Cervi polypeptide solution;
(4) sequentially carrying out vacuum concentration and spray drying treatment on the ligamentum cervi, the cauda cervi and the penis cervi polypeptide liquid, wherein the air inlet temperature of the spray drying is 170 ℃, the air outlet temperature is 88 ℃, the frequency of a high-pressure pump is 15Hz, and the atomization rotating speed is 32r/min, so as to obtain the ligamentum cervi polypeptide powder.
(c) Respectively carrying out low-temperature freeze drying, coarse crushing and nano crushing on the deer blood and the deer bone to obtain deer blood microcapsule powder and deer bone microcapsule powder;
specifically, the process c comprises the following steps,
(1) freeze drying sanguis Cervi and Os Cervi at low temperature of-77 deg.C for 12 hr to obtain lyophilized sanguis Cervi and lyophilized Os Cervi;
(2) respectively coarsely pulverizing lyophilized sanguis Cervi and lyophilized Os Cervi in FSJ-320 type pulverizer at 3700r/min to obtain sanguis Cervi coarse powder and Os Cervi coarse powder;
(3) respectively carrying out nano-crushing on the deer blood coarse powder and the deer bone coarse powder in an KQW type nano-crusher at the crusher power of 5100kw and the rotation speed of 9800r/min to obtain the deer blood micro-capsule powder and the deer bone micro-capsule powder.
S2 preparing materials containing ginseng peptide, rice bran fatty alkanol, phosphatidylserine, vitamin D, and the deer blood microcapsule powder, deer tendon, deer tail, deer penis polypeptide powder, deer bone microcapsule powder and euglena microcapsule powder obtained from S1;
specifically, the deer blood microcapsule is prepared from 25 parts by weight of deer blood microcapsule powder; 20 parts of deer sinew, deer tail and deer penis polypeptide powder; 35 parts of deer bone microcapsule powder; 6 parts of ginseng peptide; 8 parts of rice bran fatty alkanol; 9 parts of euglena microcapsule powder; 2 parts of sodium hyaluronate; 2 parts of phosphatidylserine; 0.2 part of vitamin D.
S3 the raw material of S2 is embedded by microcapsule embedding method to obtain the food.
Specifically, S3 includes, for example,
(1) mixing Euglena microcapsule powder, sanguis Cervi microcapsule powder, Os Cervi microcapsule powder, Ginseng radix peptide, testa oryzae fatty alkanol, phosphatidylserine, vitamin D and excipient, and stirring for 25min to obtain mixed powder;
(2) mixing ligamentum Cervi, cauda Cervi, penis et testis Cervi polypeptide powder, isomaltooligosaccharide syrup, sodium caseinate, sodium hyaluronate and flaxseed gum, and stirring for 25min to obtain embedding solution;
(3) introducing the mixed powder into a clean hot air fluidized bed and boiling, wherein the sterile air pressure of the fluidized bed is 3kg/cm, the flow rate of the fluidized air is 13m top year/h, the temperature of the hot air is 55 ℃, and the flow rate of the spray head air is 2m top year/h; meanwhile, introducing the embedding liquid into a pressure type nozzle for atomization, treating the mixed powder and the atomized embedding liquid by a microcapsule embedding method, contacting and bonding the mixed powder and the atomized embedding liquid into a film, drying the film by hot air, embedding and recombining for multiple times to form microcapsule powder of 40-80-mesh round particles, and preparing the microcapsule powder into different formulations of foods such as tablets, powder, granules, nutrition bars, liquid drinks and the like.
Performance test
The experiment uses a mouse swimming experiment, and the anti-fatigue effect of the product is detected through two indexes of the blood lactic acid and the lactic acid dehydrogenase (LHD)
1. Experimental Material
Healthy male mice of Kunming species with the weight of 20-25g for 2-3 months are selected as experimental animals, and are singly bred in cages, and then are divided into a control group and a deer nutritious food group, wherein each group comprises 12 mice.
2. Method and results
2.1 deer nutritious food group feeding method
The powdered deer nutritional food is mixed with the standard mouse feed in a ratio of 3:7 to prepare granulated feed which is eaten at will.
2.2 Effect on LDH Activity in mice
Before and 18d, tail blood is taken respectively and then the LDH activity is measured by the King method
The test adopts serum lactate dehydrogenase (LHD) activity and blood lactate production amount as indexes to observe the antifatigue effect of the deer nutritional food. The results are shown in Table I.
The results show that: on day 18 of the experiment, the control LDH was not significantly different from that before the experiment. On day 18 of the deer nutritious food group, the LDH activity is increased by 18 percent compared with that before the experiment, and is increased by 16 percent compared with that on day 18 of the experiment of the control group, and the obvious difference exists through t test.
2.3 Effect on blood lactate in mice
Mice were left to swim in (30. + -. 2) ℃ water for 40 minutes before and on day 25 of the experiment, respectively. Tail blood was taken before and 20 and 40 minutes after swimming and the lactic acid content in blood was measured by p-hydroxybiphenyl colorimetry. The results are shown in Table II.
The results show that: before the experiment, the control group and the deer nutritious food group have no significant difference in the blood lactic acid value (P is less than 0.05) at rest and 20 minutes and 40 minutes after exercise. On the 25 th day of the experiment of the control group, the value of the serum lactic acid is not obviously different from that before the experiment (P is more than 0.05). And 20 minutes and 40 minutes after the movement of the deer nutrition food group on the 25 th day of the experiment, the blood lactic acid value is obviously lower than that of the control group and that of the test group (P is less than 0.01).
Lactic acid is the product of anaerobic glycolysis of sugars. Prolonged vigorous exercise can cause excessive accumulation of lactic acid in the body and cause exercise fatigue. After the mouse stops moving, the blood lactic acid value can gradually recover to a normal value, and the faster the recovery, the faster the fatigue is eliminated. LDH plays a catalytic role in the metabolic process of lactate clearance. Experiments show that the deer nutritional food has the function of improving the LDH activity.
This experiment shows that: the deer nutritional food has remarkable effect on recovery of strenuous exercise fatigue.
Finally, the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all of them should be covered in the claims of the present invention.
Claims (10)
1. A sports special nutritional compound deer raw material food is characterized in that: the food is prepared from the following raw materials in parts by weight, 10-30 parts of deer blood microcapsule powder; 10-25 parts of deer sinew polypeptide powder; 10-20 parts of deer bone microcapsule powder; 10-20 parts of deer tail polypeptide powder; 10-20 parts of deer penis polypeptide powder; 5-10 parts of ginseng peptide; 3-10 parts of rice bran fatty alkanol; 5-10 parts of euglena microcapsule powder; 1-2 parts of phosphatidylserine; 2-4 parts of sodium hyaluronate; 0.1-0.2 parts of vitamin D.
2. The preparation method of the sports special nutritional compound deer raw material food as claimed in claim 1, is characterized in that: comprises the following steps of (a) carrying out,
s1 raw material pretreatment
(a) Extracting euglena, mixing and shearing the extracted euglena oil and the composite wall material solution, and then sequentially homogenizing and spray drying to obtain euglena microcapsule powder;
(b) pulverizing ligamentum Cervi, cauda Cervi and penis et testis Cervi, colloid milling, performing enzymolysis, sterilizing and solid-liquid separation, vacuum concentrating and spray drying the separated ligamentum Cervi, cauda Cervi and penis et testis Cervi polypeptide liquid respectively to obtain ligamentum Cervi, cauda Cervi and penis et testis Cervi polypeptide powder;
(c) respectively carrying out low-temperature freeze drying, coarse crushing and nano crushing on the deer blood and the deer bone to obtain deer blood microcapsule powder and deer bone microcapsule powder;
s2 preparing materials containing ginseng peptide, rice bran fatty alkanol, phosphatidylserine, vitamin D, and the deer blood microcapsule powder, deer tendon, deer tail, deer penis polypeptide powder, deer bone microcapsule powder and euglena microcapsule powder obtained from S1;
s3 embedding the raw material of S2 by microcapsule embedding method to obtain the food.
3. The preparation method of the sports special nutritional compound deer raw material food as claimed in claim 2, is characterized in that: in S1, the process a includes,
(1) by using supercritical CO2Extracting euglena at a low temperature of 35-45 ℃, under the extraction pressure of 30-40 MPa for 110-130 min to obtain euglena oil;
(2) firstly, the mass ratio is (1.5-2.5): 1, mixing the corn starch and maltodextrin to obtain a composite wall material, and dissolving the composite wall material in water to obtain a composite wall material solution with the mass concentration of 30-45%;
(3) the composite wall material solution is used as a water phase, the Euglena oil is used as an oil phase, and the mass ratio of the water phase to the oil phase is (3-5): 1, mixing and shearing to obtain a treatment solution;
(4) homogenizing the treated liquid, and spray drying the homogenized treated liquid to obtain Euglena microcapsule powder.
4. The preparation method of the sports special nutritional compound deer raw material food according to claim 3, characterized in that: in the shearing treatment, the shearing speed is 1500-2500 r/min, the shearing time is 20-40 min, and the shearing temperature is 30-45 ℃.
5. The preparation method of the sports special nutritional compound deer raw material food according to claim 3, characterized in that: in the homogenizing treatment, the homogenizing pressure is 30-40 MPa, the homogenizing temperature is 40-50 ℃, and the homogenizing times are 2-3.
6. The preparation method of the sports special nutritional compound deer raw material food according to claim 3, characterized in that: in the spray drying treatment, the inlet air temperature of spray drying is 50-170 ℃, the outlet air temperature is 70-90 ℃, the frequency of a high-pressure pump is 10-30 Hz, and the atomization rotating speed is 25-35 r/min.
7. The preparation method of the sports special nutritional compound deer raw material food as claimed in claim 2, is characterized in that: in S1, the process of b includes,
(1) mixing the deer sinew, the deer tail and the deer penis with water, and then carrying out fine crushing and colloid milling treatment to obtain a deer sinew, deer tail and deer penis suspension with the mass concentration of 10-20%;
(2) respectively carrying out enzymolysis on the deer sinew, deer tail and deer penis suspension by adopting a two-step enzymolysis method, and respectively carrying out two-step enzymolysis by using alkaline protease and neutral protease at the enzymolysis temperature of 50-55 ℃ to obtain deer sinew, deer tail and deer penis enzymolysis liquid;
(3) carrying out sterilization treatment on the deer sinew, deer tail and deer penis enzymatic hydrolysate by UHT (ultra high temperature) at the temperature of 138-143 ℃ for 7-9 s, and carrying out solid-liquid separation treatment to obtain deer sinew, deer tail and deer penis polypeptide liquid;
(4) sequentially carrying out vacuum concentration and spray drying on the deer sinew, the deer tail and the deer penis polypeptide liquid to obtain the deer sinew, the deer tail and the deer penis polypeptide powder.
8. The preparation method of the sports special nutritional compound deer raw material food as claimed in claim 2, is characterized in that: in S1, the c process includes,
(1) carrying out low-temperature freeze drying treatment on the deer blood and the deer bone respectively at the freeze drying temperature of-75 to-80 ℃ for 12-15 h to obtain freeze-dried deer blood and freeze-dried deer bone;
(2) respectively coarsely pulverizing the freeze-dried deer blood and the freeze-dried deer bone in an FSJ-320 type pulverizer at the rotating speed of 3000-4000 r/min to obtain deer blood coarse powder and deer bone coarse powder;
(3) respectively carrying out nano-crushing on the deer blood coarse powder and the deer bone coarse powder in an KQW type nano-crusher, wherein the crusher power is 4500-5500 kw and the rotation speed is 9500-10500 r/min, and obtaining the deer blood micro-capsule powder and the deer bone micro-capsule powder.
9. The preparation method of the sports special nutritional compound deer raw material food as claimed in claim 2, is characterized in that: the S3 includes the steps of,
(1) mixing euglena microcapsule powder, deer blood microcapsule powder, deer bone microcapsule powder, ginseng peptide, rice bran fatty alkanol, phosphatidylserine, vitamin D and an excipient, and stirring for 20-40 min to obtain mixed powder;
(2) mixing deer sinew, deer tail, deer penis polypeptide powder, isomaltooligosaccharide syrup, sodium hyaluronate, sodium caseinate and flaxseed gum, and stirring for 20-40 min to obtain an embedding solution;
(3) and introducing the mixed powder into a fluidized bed of clean hot air to be in a boiling state, introducing the embedding liquid into a pressure type nozzle for atomization, and treating the mixed powder and the atomized embedding liquid by adopting a microcapsule embedding method to obtain the food.
10. The preparation method of the sports special nutritional compound deer raw material food as claimed in claim 9, is characterized in that: sterile air pressure of the fluidized bed is 2-3 kg/cm, flow of fluidized air is 11-13 m/h, hot air temperature is 40-60 ℃, and flow of spray head air is 2-3 m/h.
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| CN112791103A (en) * | 2020-12-31 | 2021-05-14 | 四平华科生物技术有限责任公司 | Deer blood product and preparation method thereof |
| CN113040386A (en) * | 2020-12-03 | 2021-06-29 | 鲁芳 | Compound preparation for improving immunity and resisting fatigue and preparation method thereof |
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| CN101664187A (en) * | 2009-09-23 | 2010-03-10 | 张永富 | Deer essence nutritional composition |
| CN104840949A (en) * | 2015-06-03 | 2015-08-19 | 贵州广济堂药业有限公司 | Deer bone marrow-containing health care product for strengthening bone |
| CN111820314A (en) * | 2020-08-04 | 2020-10-27 | 中国科学院兰州化学物理研究所 | An antifatigue health product prepared from sanguis Cervi polypeptide and fructus Lycii polysaccharide |
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