CN114375943A - Umbilical cord mesenchymal stem cell storage equipment and operation method thereof - Google Patents
Umbilical cord mesenchymal stem cell storage equipment and operation method thereof Download PDFInfo
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- CN114375943A CN114375943A CN202111642631.XA CN202111642631A CN114375943A CN 114375943 A CN114375943 A CN 114375943A CN 202111642631 A CN202111642631 A CN 202111642631A CN 114375943 A CN114375943 A CN 114375943A
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- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 90
- 210000003954 umbilical cord Anatomy 0.000 title claims abstract description 90
- 238000000034 method Methods 0.000 title description 21
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 284
- 239000007788 liquid Substances 0.000 claims abstract description 247
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 142
- 238000007789 sealing Methods 0.000 claims abstract description 70
- 230000005540 biological transmission Effects 0.000 claims abstract description 27
- 238000005507 spraying Methods 0.000 claims description 19
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 238000005192 partition Methods 0.000 claims description 11
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 238000004321 preservation Methods 0.000 claims description 6
- 230000003044 adaptive effect Effects 0.000 claims description 2
- 238000011017 operating method Methods 0.000 claims 2
- 230000000694 effects Effects 0.000 abstract description 9
- 239000007921 spray Substances 0.000 description 12
- 210000000130 stem cell Anatomy 0.000 description 9
- 230000001681 protective effect Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000002035 prolonged effect Effects 0.000 description 3
- 230000003014 reinforcing effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
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Abstract
The invention discloses umbilical cord mesenchymal stem cell storage equipment, which comprises a storage box body, a sealing door, a rotating shaft, a motor, a bearing plate and a test tube, and is characterized in that: the storage box set up on the support, sealing door pass through the hinge and be connected with the storage box, the vertical bearing that passes of rotation axis, the motor setting at the outer top of storage box, be provided with the transmission shaft on the motor, with the vertical storage box that stretches into of transmission shaft, the loading board on be provided with the collar, be provided with the bearing groove on the loading board, the activity of bearing the weight of the dish place in the bearing groove, the vertical bearing hole that penetrates of test tube. According to the invention, liquid nitrogen on the upper bearing plate can conveniently flow to the adjacent bearing plates on the lower side through the through holes, so that the liquid nitrogen is fully contacted with the test tube on each bearing plate, the umbilical cord mesenchymal stem cells in the test tube are stored at low temperature, and the storage quality of the umbilical cord mesenchymal stem cells is improved.
Description
Technical Field
The invention relates to the technical field of stem cell production, in particular to umbilical cord mesenchymal stem cell storage equipment and an operation method thereof.
Background
Stem cell storage stem cells are preserved for a certain period of time by a certain method, ensuring that the function and activity of the stem cells are not obviously influenced. When the existing stem cell storage cabinet is used, the whole placing plate needs to be dragged out, stem cells in the storage cabinet are taken and placed, and the storage vessel in the storage cabinet is single and cannot deal with different types of stem cells. For example, patent application No. 202020949542.4 discloses a stem cell storage tank convenient to fix, which solves the problem that stem cells cannot be fixed and stored, but has the problems of poor universality, incapability of sterilizing the storage tank, non-constant temperature in the storage tank and poor storage effect.
Disclosure of Invention
The invention aims to solve the problems of poor universality, incapability of sterilizing the storage box, non-constant temperature in the storage box and poor storage effect of the conventional umbilical cord mesenchymal stem cell storage equipment, and provides the umbilical cord mesenchymal stem cell storage equipment which is reasonable in structural design, good in universality, constant in temperature in the storage box and good in storage effect and has a sterilization function, and the operation method thereof.
The technical scheme adopted for solving the technical problem is as follows:
the utility model provides an umbilical cord mesenchymal stem cell storage facilities, includes storage box, sealing door, rotation axis, motor, loading board, bears dish and test tube, its characterized in that: the storage box body is arranged on the bracket, a clapboard with a transverse structure is arranged in the storage box body, a liquid nitrogen box is arranged at the bottom of the storage box body, a bearing is arranged on the clapboard, the sealing door is connected with the storage box body through a hinge, the rotating shaft vertically penetrates through the bearing, a driven gear is arranged at the top of the rotating shaft, the motor is arranged at the top outside the storage box body, a transmission shaft is arranged on the motor and vertically extends into the storage box body, a driving gear is arranged on the transmission shaft and is connected with the driven gear, the motor drives the transmission shaft and the driving gear on the transmission shaft to rotate, the driving gear drives the driven gear to rotate, the driven gear drives the rotating shaft and the bearing plate arranged on the rotating shaft to rotate, so that the bearing plate drives the test tube to rotate, and liquid nitrogen conveniently flows to the bearing plate adjacent to the lower side through the through holes on the bearing plate on the upper layer, the liquid nitrogen is fully contacted with the test tubes on each layer of bearing plate to store the umbilical cord mesenchymal stem cells in the test tubes at low temperature, the storage quality of the umbilical cord mesenchymal stem cells is improved, the bearing plate is provided with a mounting ring, the bearing plate is provided with a bearing groove, the mounting ring is sleeved on a rotating shaft through a mounting bolt, the bearing disc is movably arranged in the bearing groove and is provided with a bearing hole, the mounting ring on the bearing plate is mounted on the rotating shaft through the mounting bolt, the stability of the mounting ring and the bearing plate on the rotating shaft is improved, the stability of the bearing plate in the rotating process along with the rotating shaft can also be improved, the position of the bearing plate on the rotating shaft is adjusted through the mounting ring and the mounting bolt according to the length of the test tubes, the distance between the upper and the lower bearing plates can adapt to the height of the test tubes, and the universality of the storage device is improved, liquid nitrogen on the upper loading board of being convenient for through the thru hole flows to the loading board that the downside is adjacent on, makes liquid nitrogen fully with the test tube contact on the every layer loading board, carries out low temperature to the umbilical cord mesenchymal stem cell in the test tube and stores, has improved the storage quality of umbilical cord mesenchymal stem cell, is provided with the dog in the bearing groove and can avoids bearing the direct follow bearing groove of dish and fall out, improves and bears the stability of dish on the loading board, the vertical bearing hole that penetrates of test tube to be provided with the sealing plug at the test tube top, the umbilical cord mesenchymal stem cell that will need to store puts into the test tube, the sealing plug can improve the leakproofness of test tube, avoids umbilical cord mesenchymal stem cell to flow from the test tube, reinforcing umbilical cord mesenchymal stem cell's storage quality.
As preferred, the bin lateral wall, all be provided with the heat preservation in top and the baffle, be provided with the lamp stand on the storage box inner wall of baffle below, be provided with ultraviolet lamp between lamp stand and lamp stand, and be provided with parcel ultraviolet lamp's safety cover on storing the box inner wall, can make the interior stable low temperature environment that maintains of storage box through the heat preservation, avoid the external heat of storage box to get into in the bin, improve the storage quality of umbilical cord mesenchymal stem cell in the bin, can carry out ultraviolet irradiation sterilization in the storage box through ultraviolet lamp, avoid the microorganism in the storage box to cause the pollution to umbilical cord mesenchymal stem cell, improve the storage quality of umbilical cord mesenchymal stem cell, can provide the protection to ultraviolet lamp through the safety cover, prolong ultraviolet lamp's life.
Preferably, the sealing door on be provided with the sealing strip, through the sealing strip reinforcing sealing door and store the leakproofness between the box, improve microthermal stability in the bin body, and then improve umbilical cord mesenchymal stem cell's storage effect.
Preferably, the rotating shaft is of a hollow structure, a liquid spraying chamber is arranged on the lower side of the partition plate, a nozzle is arranged on the liquid spraying chamber, a liquid nitrogen pump is arranged at the top in the storage box body, a liquid suction pipe and a liquid conveying pipe are arranged on the liquid nitrogen pump, the liquid suction pipe extends into the hollow rotating shaft from the top end of the rotating shaft and is connected with the liquid spraying chamber, the liquid nitrogen pump enables negative pressure to be generated in the hollow rotating shaft through the liquid suction pipe, liquid nitrogen in the liquid nitrogen box enters the liquid suction pipe through a liquid suction cylinder and is conveyed into the liquid spraying chamber from the liquid conveying pipe and is sprayed out from the nozzle to a bearing plate in a bearing groove on the bearing plate, the liquid nitrogen on the bearing plate flows downwards along the outer wall of the test tube or the edge of the bearing plate and flows into a liquid nitrogen box at the bottom of the storage box body, circular flow of the liquid nitrogen is realized, on the other hand, the bearing plate drives the test tube to rotate, so that the liquid nitrogen sprayed by the nozzle uniformly falls on the bearing plate on the uppermost layer, liquid nitrogen on the bearing plate on the uppermost layer flows to the bearing plates adjacent to the lower side through the through holes and finally flows into the liquid nitrogen box, so that the liquid nitrogen is fully contacted with the test tubes on each layer of bearing plate, umbilical cord mesenchymal stem cells in the test tubes are stored at low temperature, and the storage quality of the umbilical cord mesenchymal stem cells is improved.
Preferably, the bottom end of the rotating shaft is connected with a liquid suction barrel through threads, the liquid suction barrel vertically extends into the liquid nitrogen box, a liquid suction hole is formed in the side wall of the bottom of the liquid suction barrel, the liquid suction barrel is connected with the rotating shaft through threads, the liquid suction barrel is convenient to mount or replace on the rotating shaft, the length of the rotating shaft can be prolonged by mounting the liquid suction barrel, the bottom end of the liquid suction barrel extends into the liquid nitrogen box to suck liquid nitrogen out, and liquid nitrogen in the liquid nitrogen box can quickly enter the hollow rotating shaft through the liquid suction hole in the side wall of the bottom of the liquid suction barrel, so that the liquid nitrogen can quickly flow; after the liquid suction cylinder is taken down from the rotating shaft, a certain distance is reserved between the bottom end of the rotating shaft and the bottom in the storage box body, so that the bearing plate can be conveniently mounted or dismounted on the rotating shaft, and the working efficiency is improved.
Preferably, a sealing ring is arranged between the pipette and the inner wall of the hollow rotating shaft, the sealing ring improves the sealing performance between the pipette and the rotating shaft, liquid nitrogen in the rotating shaft is prevented from flowing out from the space between the pipette and the rotating shaft, on the other hand, the rotating shaft can conveniently rotate around the pipette through the sealing ring, and the stability of the rotating shaft in the rotating process is improved.
Preferably, the bearing plate is arranged into a structure capable of adjusting the position on the rotating shaft through the mounting ring and the mounting bolt, the bearing plate is provided with through holes, the bearing groove is internally provided with a stop block, the mounting ring on the bearing plate is mounted on the rotating shaft through the mounting bolt, the stability of the mounting ring and the bearing plate on the rotating shaft is improved, the stability of the bearing plate in the rotating process along with the rotating shaft is also improved, the position of the bearing plate on the rotating shaft is adjusted through the mounting ring and the mounting bolt according to the length of the test tube, the distance between the upper bearing plate and the lower bearing plate can adapt to the height of the test tube, the universality of the storage device is improved, liquid nitrogen on the upper bearing plate can conveniently flow to the bearing plate adjacent to the lower side through the through holes, the liquid nitrogen is fully contacted with the test tube on each layer of the bearing plate, and the umbilical cord mesenchymal stem cells in the test tube are stored at low temperature, the storage quality of umbilical cord mesenchymal stem cells is improved, the baffle plate is arranged in the bearing groove, the bearing plate can be prevented from directly falling out of the bearing groove, and the stability of the bearing plate on the bearing plate is improved.
As preferred, bear and be provided with the handle on the dish, and will bear the dish and set up to the structure that can take out in the bearing groove, will bear and pass through threaded connection between dish and the bearing groove inner wall, be convenient for bear the installation of dish in the bearing groove or take out, be convenient for bear the dish at the bearing groove internal rotation through the handle and bear the dish, improve the efficiency of bearing the dish and installing or taking out in the bearing groove, reduce operating personnel's intensity of labour, according to the size of test tube, select the dish that bears that has corresponding bearing hole, not only can improve the stability of test tube in the bearing hole, also enable the storage device and be applicable to the test tube of variation in size, storage device's commonality has been improved.
Preferably, bear and be provided with the slipmat on the carrier plate on the inner wall of the carrier hole, the test tube that will be equipped with umbilical cord mesenchymal stem cell inserts in the carrier hole, can increase the frictional force that the test tube received in the carrier hole through the slipmat, improves the stability of test tube in the carrier hole, and then improves the stability of umbilical cord mesenchymal stem cell in the storage process.
An operation method suitable for umbilical cord mesenchymal stem cell storage equipment is characterized by comprising the following steps:
firstly, adjusting the positions of the bearing plates on the rotating shaft through the mounting rings and the mounting bolts according to the length of the test tubes to enable the distance between the upper bearing plate and the lower bearing plate to be adaptive to the height of the test tubes, selecting the bearing plate with the corresponding bearing hole according to the size of the test tubes, and checking whether the line of the umbilical cord mesenchymal stem cell storage equipment is complete;
secondly, closing the sealing door, opening an ultraviolet lamp, carrying out ultraviolet irradiation sterilization on the storage box body, opening the sealing door after sterilization, inserting the test tube filled with the umbilical cord mesenchymal stem cells into the bearing hole, putting the bearing disc into the bearing groove, and closing the sealing door;
injecting liquid nitrogen into the liquid nitrogen box, starting a liquid nitrogen pump, enabling the liquid nitrogen pump to generate negative pressure in the hollow rotating shaft through a liquid suction pipe, enabling the liquid nitrogen in the liquid nitrogen box to enter the liquid suction pipe through a liquid suction cylinder, conveying the liquid nitrogen into a liquid spraying chamber from a liquid conveying pipe, spraying the liquid nitrogen from a nozzle, and spraying the liquid nitrogen onto a bearing disc in a bearing groove on the bearing plate;
fourthly, starting a motor, driving a transmission shaft and a driving gear on the transmission shaft to rotate by the motor, driving a driven gear to rotate by the driving gear, driving a rotating shaft and a bearing plate arranged on the rotating shaft to rotate by the driven gear, driving a test tube to rotate by a bearing plate, and fully contacting the test tube with liquid nitrogen sprayed out from a nozzle;
and fifthly, before taking out the umbilical cord mesenchymal stem cells, closing the motor and the liquid nitrogen pump, opening the sealing door, quickly taking out the bearing disc from the bearing groove, and quickly closing the sealing door after taking out the bearing disc.
Has the advantages that: the invention leads the liquid nitrogen pump to generate negative pressure in the hollow rotating shaft through the liquid suction pipe, liquid nitrogen in the liquid nitrogen box enters the liquid suction pipe through the liquid suction cylinder and is conveyed to the liquid spraying chamber from the liquid conveying pipe, and sprayed out from the nozzle to be sprayed on a bearing plate in a bearing groove on the bearing plate, liquid nitrogen on the bearing plate flows downwards along the outer wall of the test tube or the edge of the bearing plate and flows into a liquid nitrogen box at the bottom of the storage box body to realize the circular flow of the liquid nitrogen, on the other hand, the bearing plate drives the test tube to rotate, so that the liquid nitrogen sprayed by the nozzle uniformly falls on the bearing plate at the uppermost layer, the liquid nitrogen on the bearing plate at the uppermost layer flows onto the bearing plate adjacent to the lower side through the through holes and finally flows into the liquid nitrogen box, so that the liquid nitrogen is fully contacted with the test tube on each layer of bearing plate, the umbilical cord mesenchymal stem cells in the test tube are stored at low temperature, so that the storage quality of the umbilical cord mesenchymal stem cells is improved.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a partial structural view illustrating a connection structure of a rotation shaft and a pipette in accordance with the present invention.
FIG. 3 is a partial schematic structural view of the present invention, illustrating a connection structure of a rotation shaft and a liquid suction cylinder.
Fig. 4 is a partial structural schematic view of the present invention, illustrating a connection structure of a driving gear and a driven gear.
FIG. 5 is a partial schematic structural view illustrating a connection structure of the carrier plate and the through holes according to the present invention.
FIG. 6 is a partial schematic structural view illustrating a connection structure of a carrier plate and a carrier tray according to the present invention.
FIG. 7 is a partial structural diagram of the present invention, illustrating the connection structure of the carrier tray and the carrier hole.
Fig. 8 is a schematic diagram of another embodiment of the present invention.
FIG. 9 is a partial schematic structural view of FIG. 8 showing a connection structure of a tray and a drain tank according to the present invention.
In the figure: 1. the liquid storage device comprises a storage box body, 2 parts of a sealing door, 3 parts of a rotating shaft, 4 parts of a motor, 5 parts of a bearing plate, 6 parts of a bearing plate, 7 parts of a test tube, 8 parts of a bracket, 9 parts of a partition plate, 10 parts of a liquid nitrogen box, 11 parts of a lamp holder, 12 parts of an ultraviolet lamp, 13 parts of a protective cover, 14 parts of a bearing, 15 parts of a heat preservation layer, 16 parts of a liquid nitrogen pump, 17 parts of a pipette, 18 parts of a liquid conveying pipe, 19 parts of a liquid spraying chamber, 20 parts of a nozzle, 21 parts of a hinge, 22 parts of a sealing strip, 23 parts of a driven gear, 24 parts of a liquid suction cylinder, 25 parts of a liquid suction hole, 26 parts of a transmission shaft, 27 parts of a driving gear, 28 parts of a mounting ring, 29 parts of a bearing groove, 30 parts of a through hole, 31 parts of a mounting bolt, 32 parts of a stop block, 33 parts of a handle, 34 parts of a bearing hole, 35 parts of a mat, 36 parts of a sealing plug, 37 parts of a sealing ring, 38 parts of a connecting rod, 39 parts of a supporting plate and 40 parts of a liquid discharge groove.
Detailed Description
The invention will be described in more detail below with reference to the accompanying drawings.
The first embodiment is as follows:
as shown in figures 1-7: the utility model provides an umbilical cord mesenchymal stem cell storage facilities, includes storage box 1, sealing door 2, rotation axis 3, motor 4, loading board 5, bears dish 6 and test tube 7, its characterized in that: the storage box body 1 is arranged on a bracket 8, a partition plate 9 with a transverse structure is arranged in the storage box body 1, a liquid nitrogen box 10 is arranged at the bottom of the storage box body 1, a bearing 14 is arranged on the partition plate 9, the sealing door 2 is connected with the storage box body 1 through a hinge 21, the rotating shaft 3 vertically penetrates through the bearing 14, a driven gear 23 is arranged at the top of the rotating shaft 3, the motor 4 is arranged at the outer top of the storage box body 1, a transmission shaft 26 is arranged on the motor 4, the transmission shaft 26 vertically extends into the storage box body 1, a driving gear 27 is arranged on the transmission shaft 26, the driving gear 27 is connected with the driven gear 23, the motor 4 drives the transmission shaft 26 and the driving gear 27 on the transmission shaft 26 to rotate, the driving gear 27 drives the driven gear 23 to rotate, the driven gear 23 drives the rotating shaft 3 and the bearing plate 5 arranged on the rotating shaft 3 to rotate, the bearing plate 6 drives the test tubes 7 to rotate, liquid nitrogen on the upper bearing plate 5 can conveniently flow to the bearing plates 5 adjacent to the lower side through the through holes 30, the liquid nitrogen is fully contacted with the test tubes 7 on each layer of the bearing plates 5, the umbilical cord mesenchymal stem cells in the test tubes 7 are stored at low temperature, the storage quality of the umbilical cord mesenchymal stem cells is improved, the bearing plates 5 are provided with the mounting rings 28, the bearing plates 5 are provided with the bearing grooves 29, the mounting rings 28 are sleeved on the rotating shaft 3 through the mounting bolts 31, the bearing plate 6 is movably arranged in the bearing grooves 29, the bearing plates 6 are provided with the bearing holes 34, the mounting rings 28 on the bearing plates 5 are arranged on the rotating shaft 3 through the mounting bolts 31, the stability of the mounting rings 28 and the bearing plates 5 on the rotating shaft 3 is improved, and the stability of the bearing plates 5 in the rotating process along with the rotating shaft 3 can also be improved, according to the length of the test tube 7, the positions of the bearing plates 5 on the rotating shaft 3 are adjusted through the mounting rings 28 and the mounting bolts 31, so that the distance between the upper bearing plate 5 and the lower bearing plate 5 can adapt to the height of the test tube 7, the universality of the storage device is improved, liquid nitrogen on the upper bearing plate 5 can flow to the bearing plate 5 adjacent to the lower side through the through holes 30, the liquid nitrogen is fully contacted with the test tube 7 on each layer of bearing plate 5, the umbilical cord mesenchymal stem cells in the test tube 7 are stored at low temperature, the storage quality of the umbilical cord mesenchymal stem cells is improved, the baffle 32 is arranged in the bearing groove 29, the bearing plate 6 can be prevented from directly falling out of the bearing groove 29, the stability of the bearing plate 6 on the bearing plate 5 is improved, the test tube 7 vertically penetrates into the bearing hole 34, the top of the test tube 7 is provided with the sealing plug 36, the umbilical cord mesenchymal stem cells to be stored are placed in the test tube 7, the sealing plug 36 can improve the sealing performance of the test tube 7, prevent umbilical cord mesenchymal stem cells from flowing out of the test tube 7, and enhance the storage quality of the umbilical cord mesenchymal stem cells.
Preferably, the side wall, the top and the partition 9 of the storage box body 1 are all provided with an insulating layer 15, a lamp holder 11 is arranged on the inner wall of the storage box body 1 below the clapboard 9, an ultraviolet lamp 12 is arranged between the lamp holder 11 and the lamp holder 11, a protective cover 13 for wrapping the ultraviolet lamp 12 is arranged on the inner wall of the storage box body 1, the stable low-temperature environment can be maintained in the storage box body 1 through the heat preservation layer 15, the heat outside the storage box body 1 is prevented from entering the storage box body 1, the storage quality of the umbilical cord mesenchymal stem cells in the storage box body 1 is improved, the storage box body 1 can be sterilized by ultraviolet irradiation through the ultraviolet lamp 12, the pollution of the umbilical cord mesenchymal stem cells caused by microorganisms in the storage box body 1 is avoided, the storage quality of the umbilical cord mesenchymal stem cells is improved, the protective cover 13 can protect the ultraviolet lamp 12, and the service life of the ultraviolet lamp 12 is prolonged.
Preferably, the sealing door 2 on be provided with sealing strip 22, strengthen the leakproofness between sealing door 2 and the storage box 1 through sealing strip 22, improve the stability of low temperature in the storage box 1, and then improve the storage effect of umbilical cord mesenchymal stem cell.
Preferably, the rotating shaft 3 is hollow, the spray chamber 19 is arranged below the partition plate 9, the spray nozzle 20 is arranged on the spray chamber 19, the liquid nitrogen pump 16 is arranged at the top in the storage box 1, the liquid nitrogen pump 16 is provided with a liquid suction pipe 17 and a liquid conveying pipe 18, the liquid suction pipe 17 extends into the hollow rotating shaft 3 from the top end of the rotating shaft 3, the liquid conveying pipe 18 is connected with the spray chamber 19, the liquid nitrogen pump 16 generates negative pressure in the hollow rotating shaft 3 through the liquid suction pipe 17, liquid nitrogen in the liquid nitrogen box 10 enters the liquid suction pipe 17 through a liquid suction cylinder 24, is conveyed into the spray chamber 19 from the liquid conveying pipe 18 and is sprayed out of the spray nozzle 20 to the carrier tray 6 in the carrier groove 29 on the carrier plate 5, the liquid nitrogen on the carrier tray 6 flows downwards along the outer wall of the test tube 7 or the edge of the carrier tray 6 and flows into the liquid nitrogen box 10 at the bottom of the storage box 1, realized the circulation flow of liquid nitrogen, on the other hand, bear the weight of the dish 6 and drive test tube 7 rotatory, make the even loading board 5 that falls on the superiors of liquid nitrogen that nozzle 20 sprayed, liquid nitrogen on the loading board 5 of the superiors flows to the downside adjacent loading board 5 through thru hole 30 on, finally flows into in liquid nitrogen box 10, makes liquid nitrogen fully contact with test tube 7 on each layer loading board 5, carries out low temperature storage to the umbilical cord mesenchymal stem cell in the test tube 7, has improved umbilical cord mesenchymal stem cell's storage quality.
Preferably, the bottom end of the rotating shaft 3 is connected with a liquid suction cylinder 24 through threads, the liquid suction cylinder 24 vertically extends into the liquid nitrogen box 10, a liquid suction hole 25 is formed in the side wall of the bottom of the liquid suction cylinder 24, the liquid suction cylinder 24 is connected with the rotating shaft 3 through threads, the liquid suction cylinder 24 is convenient to mount or replace on the rotating shaft 3, the liquid suction cylinder 24 is mounted to prolong the length of the rotating shaft 3, the bottom end of the liquid suction cylinder 24 extends into the liquid nitrogen box 10 to suck liquid nitrogen out, and the liquid nitrogen in the liquid nitrogen box 10 can quickly enter the hollow rotating shaft 3 through the liquid suction hole 25 in the side wall of the bottom of the liquid suction cylinder 24, so that the liquid nitrogen can quickly flow; after the liquid suction cylinder 24 is taken down from the rotating shaft 3, a certain distance is reserved between the bottom end of the rotating shaft 3 and the bottom in the storage box body 1, so that the bearing plate 5 can be conveniently mounted or dismounted on the rotating shaft 3, and the working efficiency is improved.
Preferably, a sealing ring 37 is arranged between the pipette 17 and the inner wall of the hollow rotating shaft 3, the sealing ring 37 improves the sealing performance between the pipette 17 and the rotating shaft 3, so that liquid nitrogen in the rotating shaft 3 is prevented from flowing out from between the pipette 17 and the rotating shaft 3, and on the other hand, the sealing ring 37 is also convenient for the rotating shaft 3 to rotate around the pipette 17, so that the stability of the rotating shaft 3 in the rotating process is improved.
Preferably, the carrying plate 5 is configured to be adjustable in position on the rotating shaft 3 through the mounting ring 28 and the mounting bolt 31, the carrying plate 5 is provided with the through hole 30, the bearing groove 29 is provided with the stopper 32, the mounting ring 28 on the carrying plate 5 is mounted on the rotating shaft 3 through the mounting bolt 31, not only the stability of the mounting ring 28 and the carrying plate 5 on the rotating shaft 3 is improved, but also the stability of the carrying plate 5 in the rotating process along with the rotating shaft 3 is improved, the position of the carrying plate 5 on the rotating shaft 3 is adjusted through the mounting ring 28 and the mounting bolt 31 according to the length of the test tube 7, the distance between the upper and lower carrying plates 5 can be adapted to the height of the test tube 7, the universality of the storage device is improved, the liquid nitrogen on the upper carrying plate 5 can flow to the carrying plate 5 adjacent to the lower side through the through hole 30, and the liquid nitrogen is fully contacted with the test tube 7 on each layer of the carrying plate 5, carry out low temperature to the umbilical cord mesenchymal stem cell in the test tube 7 and store, improved umbilical cord mesenchymal stem cell's storage quality, be provided with dog 32 in bearing groove 29 and can avoid bearing dish 6 directly to fall out from bearing groove 29, improve and bear the stability of dish 6 on loading board 5.
Preferably, the carrying tray 6 is provided with a handle 33, the carrying tray 6 is set to be a structure capable of being taken out of the carrying groove 29, the carrying tray 6 is connected with the inner wall of the carrying groove 29 through threads, so that the carrying tray 6 can be conveniently installed or taken out of the carrying groove 29, the carrying tray 6 can be conveniently rotated in the carrying groove 29 through the handle 33, the installation or taking-out efficiency of the carrying tray 6 in the carrying groove 29 is improved, the labor intensity of an operator is reduced, the carrying tray 6 with the corresponding carrying hole 34 is selected according to the size of the test tube 7, the stability of the test tube 7 in the carrying hole 34 can be improved, the storage device can be suitable for test tubes 7 with different sizes, and the universality of the storage device is improved.
Preferably, the inner wall of the bearing hole 34 on the bearing tray 6 is provided with an anti-slip pad 35, the test tube 7 with the umbilical cord mesenchymal stem cells is inserted into the bearing hole 34, the friction force of the test tube 7 in the bearing hole 34 can be increased through the anti-slip pad 35, the stability of the test tube 7 in the bearing hole 34 is improved, and the stability of the umbilical cord mesenchymal stem cells in the storage process is further improved.
An operation method suitable for umbilical cord mesenchymal stem cell storage equipment is characterized by comprising the following steps:
firstly, adjusting the positions of the bearing plates 5 on the rotating shaft 3 through the mounting rings 28 and the mounting bolts 31 according to the length of the test tubes 7, enabling the distance between the upper bearing plate 5 and the lower bearing plate 5 to be suitable for the height of the test tubes 7, selecting the bearing plate 6 with the corresponding bearing hole 34 according to the size of the test tubes 7, and checking whether the circuit of the umbilical cord mesenchymal stem cell storage equipment is complete;
secondly, closing the sealing door 2, opening the ultraviolet lamp 12, performing ultraviolet irradiation sterilization on the storage box body 1, after sterilization, opening the sealing door 2, inserting the test tube 7 filled with the umbilical cord mesenchymal stem cells into the bearing hole 34, placing the bearing disc 6 into the bearing groove 29, and closing the sealing door 2;
thirdly, injecting liquid nitrogen into the liquid nitrogen box 10, starting the liquid nitrogen pump 16, enabling the liquid nitrogen pump 16 to generate negative pressure in the hollow rotating shaft 3 through the liquid suction pipe 17, enabling the liquid nitrogen in the liquid nitrogen box 10 to enter the liquid suction pipe 17 through the liquid suction barrel 24, conveying the liquid nitrogen into the liquid suction chamber 19 from the liquid conveying pipe 18, spraying the liquid nitrogen out of the nozzle 20, and spraying the liquid nitrogen onto the bearing plate 6 in the bearing groove 29 on the bearing plate 5;
fourthly, starting the motor 4, driving the transmission shaft 26 and the driving gear 27 on the transmission shaft 26 to rotate by the motor 4, driving the driven gear 23 to rotate by the driving gear 27, driving the rotating shaft 3 and the bearing plate 5 arranged on the rotating shaft 3 to rotate by the driven gear 23, driving the test tube 7 to rotate by the bearing plate 6, and fully contacting the test tube 7 with the liquid nitrogen sprayed out by the nozzle 20;
fifthly, before taking out the umbilical cord mesenchymal stem cells, the motor 4 and the liquid nitrogen pump 16 are closed, the sealing door 2 is opened, the bearing disc 6 is quickly taken out from the bearing groove 29, and the sealing door 2 is quickly closed after the bearing disc 6 is taken out.
Example two:
as shown in figures 8-9: the utility model provides an umbilical cord mesenchymal stem cell storage facilities, includes storage box 1, sealing door 2, rotation axis 3, motor 4, loading board 5, bears dish 6 and test tube 7, its characterized in that: the storage box body 1 is arranged on a bracket 8, a partition plate 9 with a transverse structure is arranged in the storage box body 1, a liquid nitrogen box 10 is arranged at the bottom of the storage box body 1, a bearing 14 is arranged on the partition plate 9, the sealing door 2 is connected with the storage box body 1 through a hinge 21, the rotating shaft 3 vertically penetrates through the bearing 14, a driven gear 23 is arranged at the top of the rotating shaft 3, the motor 4 is arranged at the outer top of the storage box body 1, a transmission shaft 26 is arranged on the motor 4, the transmission shaft 26 vertically extends into the storage box body 1, a driving gear 27 is arranged on the transmission shaft 26, the driving gear 27 is connected with the driven gear 23, the motor 4 drives the transmission shaft 26 and the driving gear 27 on the transmission shaft 26 to rotate, the driving gear 27 drives the driven gear 23 to rotate, the driven gear 23 drives the rotating shaft 3 and the bearing plate 5 arranged on the rotating shaft 3 to rotate, the bearing plate 6 drives the test tubes 7 to rotate, liquid nitrogen on the upper bearing plate 5 can conveniently flow to the bearing plates 5 adjacent to the lower side through the through holes 30, the liquid nitrogen is fully contacted with the test tubes 7 on each layer of the bearing plates 5, the umbilical cord mesenchymal stem cells in the test tubes 7 are stored at low temperature, the storage quality of the umbilical cord mesenchymal stem cells is improved, the bearing plates 5 are provided with the mounting rings 28, the bearing plates 5 are provided with the bearing grooves 29, the mounting rings 28 are sleeved on the rotating shaft 3 through the mounting bolts 31, the bearing plate 6 is movably arranged in the bearing grooves 29, the bearing plates 6 are provided with the bearing holes 34, the mounting rings 28 on the bearing plates 5 are arranged on the rotating shaft 3 through the mounting bolts 31, the stability of the mounting rings 28 and the bearing plates 5 on the rotating shaft 3 is improved, and the stability of the bearing plates 5 in the rotating process along with the rotating shaft 3 can also be improved, according to the length of the test tube 7, the positions of the bearing plates 5 on the rotating shaft 3 are adjusted through the mounting rings 28 and the mounting bolts 31, so that the distance between the upper bearing plate 5 and the lower bearing plate 5 can adapt to the height of the test tube 7, the universality of the storage device is improved, liquid nitrogen on the upper bearing plate 5 can flow to the bearing plate 5 adjacent to the lower side through the through holes 30, the liquid nitrogen is fully contacted with the test tube 7 on each layer of bearing plate 5, the umbilical cord mesenchymal stem cells in the test tube 7 are stored at low temperature, the storage quality of the umbilical cord mesenchymal stem cells is improved, the baffle 32 is arranged in the bearing groove 29, the bearing plate 6 can be prevented from directly falling out of the bearing groove 29, the stability of the bearing plate 6 on the bearing plate 5 is improved, the test tube 7 vertically penetrates into the bearing hole 34, the top of the test tube 7 is provided with the sealing plug 36, the umbilical cord mesenchymal stem cells to be stored are placed in the test tube 7, the sealing plug 36 can improve the sealing performance of the test tube 7, prevent umbilical cord mesenchymal stem cells from flowing out of the test tube 7, and enhance the storage quality of the umbilical cord mesenchymal stem cells.
Preferably, the side wall, the top and the partition 9 of the storage box body 1 are all provided with an insulating layer 15, a lamp holder 11 is arranged on the inner wall of the storage box body 1 below the clapboard 9, an ultraviolet lamp 12 is arranged between the lamp holder 11 and the lamp holder 11, a protective cover 13 for wrapping the ultraviolet lamp 12 is arranged on the inner wall of the storage box body 1, the stable low-temperature environment can be maintained in the storage box body 1 through the heat preservation layer 15, the heat outside the storage box body 1 is prevented from entering the storage box body 1, the storage quality of the umbilical cord mesenchymal stem cells in the storage box body 1 is improved, the storage box body 1 can be sterilized by ultraviolet irradiation through the ultraviolet lamp 12, the pollution of the umbilical cord mesenchymal stem cells caused by microorganisms in the storage box body 1 is avoided, the storage quality of the umbilical cord mesenchymal stem cells is improved, the protective cover 13 can protect the ultraviolet lamp 12, and the service life of the ultraviolet lamp 12 is prolonged.
Preferably, the sealing door 2 on be provided with sealing strip 22, strengthen the leakproofness between sealing door 2 and the storage box 1 through sealing strip 22, improve the stability of low temperature in the storage box 1, and then improve the storage effect of umbilical cord mesenchymal stem cell.
Preferably, the rotating shaft 3 is hollow, the spray chamber 19 is arranged below the partition plate 9, the spray nozzle 20 is arranged on the spray chamber 19, the liquid nitrogen pump 16 is arranged at the top in the storage box 1, the liquid nitrogen pump 16 is provided with a liquid suction pipe 17 and a liquid conveying pipe 18, the liquid suction pipe 17 extends into the hollow rotating shaft 3 from the top end of the rotating shaft 3, the liquid conveying pipe 18 is connected with the spray chamber 19, the liquid nitrogen pump 16 generates negative pressure in the hollow rotating shaft 3 through the liquid suction pipe 17, liquid nitrogen in the liquid nitrogen box 10 enters the liquid suction pipe 17 through a liquid suction cylinder 24, is conveyed into the spray chamber 19 from the liquid conveying pipe 18 and is sprayed out of the spray nozzle 20 to the carrier tray 6 in the carrier groove 29 on the carrier plate 5, the liquid nitrogen on the carrier tray 6 flows downwards along the outer wall of the test tube 7 or the edge of the carrier tray 6 and flows into the liquid nitrogen box 10 at the bottom of the storage box 1, realized the circulation flow of liquid nitrogen, on the other hand, bear the weight of the dish 6 and drive test tube 7 rotatory, make the even loading board 5 that falls on the superiors of liquid nitrogen that nozzle 20 sprayed, liquid nitrogen on the loading board 5 of the superiors flows to the downside adjacent loading board 5 through thru hole 30 on, finally flows into in liquid nitrogen box 10, makes liquid nitrogen fully contact with test tube 7 on each layer loading board 5, carries out low temperature storage to the umbilical cord mesenchymal stem cell in the test tube 7, has improved umbilical cord mesenchymal stem cell's storage quality.
Preferably, the bottom end of the rotating shaft 3 is connected with a liquid suction cylinder 24 through threads, the liquid suction cylinder 24 vertically extends into the liquid nitrogen box 10, a liquid suction hole 25 is formed in the side wall of the bottom of the liquid suction cylinder 24, the liquid suction cylinder 24 is connected with the rotating shaft 3 through threads, the liquid suction cylinder 24 is convenient to mount or replace on the rotating shaft 3, the liquid suction cylinder 24 is mounted to prolong the length of the rotating shaft 3, the bottom end of the liquid suction cylinder 24 extends into the liquid nitrogen box 10 to suck liquid nitrogen out, and the liquid nitrogen in the liquid nitrogen box 10 can quickly enter the hollow rotating shaft 3 through the liquid suction hole 25 in the side wall of the bottom of the liquid suction cylinder 24, so that the liquid nitrogen can quickly flow; after the liquid suction cylinder 24 is taken down from the rotating shaft 3, a certain distance is reserved between the bottom end of the rotating shaft 3 and the bottom in the storage box body 1, so that the bearing plate 5 can be conveniently mounted or dismounted on the rotating shaft 3, and the working efficiency is improved.
Preferably, a sealing ring 37 is arranged between the pipette 17 and the inner wall of the hollow rotating shaft 3, the sealing ring 37 improves the sealing performance between the pipette 17 and the rotating shaft 3, so that liquid nitrogen in the rotating shaft 3 is prevented from flowing out from between the pipette 17 and the rotating shaft 3, and on the other hand, the sealing ring 37 is also convenient for the rotating shaft 3 to rotate around the pipette 17, so that the stability of the rotating shaft 3 in the rotating process is improved.
Preferably, the carrying plate 5 is configured to be adjustable in position on the rotating shaft 3 through the mounting ring 28 and the mounting bolt 31, the carrying plate 5 is provided with the through hole 30, the bearing groove 29 is provided with the stopper 32, the mounting ring 28 on the carrying plate 5 is mounted on the rotating shaft 3 through the mounting bolt 31, not only the stability of the mounting ring 28 and the carrying plate 5 on the rotating shaft 3 is improved, but also the stability of the carrying plate 5 in the rotating process along with the rotating shaft 3 is improved, the position of the carrying plate 5 on the rotating shaft 3 is adjusted through the mounting ring 28 and the mounting bolt 31 according to the length of the test tube 7, the distance between the upper and lower carrying plates 5 can be adapted to the height of the test tube 7, the universality of the storage device is improved, the liquid nitrogen on the upper carrying plate 5 can flow to the carrying plate 5 adjacent to the lower side through the through hole 30, and the liquid nitrogen is fully contacted with the test tube 7 on each layer of the carrying plate 5, carry out low temperature to the umbilical cord mesenchymal stem cell in the test tube 7 and store, improved umbilical cord mesenchymal stem cell's storage quality, be provided with dog 32 in bearing groove 29 and can avoid bearing dish 6 directly to fall out from bearing groove 29, improve and bear the stability of dish 6 on loading board 5.
Preferably, the carrying tray 6 is provided with a handle 33, the carrying tray 6 is set to be a structure capable of being taken out of the carrying groove 29, the carrying tray 6 is connected with the inner wall of the carrying groove 29 through threads, so that the carrying tray 6 can be conveniently installed or taken out of the carrying groove 29, the carrying tray 6 can be conveniently rotated in the carrying groove 29 through the handle 33, the installation or taking-out efficiency of the carrying tray 6 in the carrying groove 29 is improved, the labor intensity of an operator is reduced, the carrying tray 6 with the corresponding carrying hole 34 is selected according to the size of the test tube 7, the stability of the test tube 7 in the carrying hole 34 can be improved, the storage device can be suitable for test tubes 7 with different sizes, and the universality of the storage device is improved.
Preferably, the inner wall of the bearing hole 34 on the bearing tray 6 is provided with an anti-slip pad 35, the test tube 7 with the umbilical cord mesenchymal stem cells is inserted into the bearing hole 34, the friction force of the test tube 7 in the bearing hole 34 can be increased through the anti-slip pad 35, the stability of the test tube 7 in the bearing hole 34 is improved, and the stability of the umbilical cord mesenchymal stem cells in the storage process is further improved.
Preferably, the bottom side of the bearing plate 5 is provided with the connecting rod 38, the bottom of the connecting rod 38 is provided with the supporting plate 39, the supporting plate 39 is provided with a liquid discharge groove 40, the bottom end of the test tube 7 can be limited by the supporting plate 39, the situation that the test tube 7 is thrown out in the rotating process is avoided, the stability of the test tube 7 is improved, the liquid nitrogen on the supporting plate 39 can flow downwards quickly through the liquid discharge groove 40, the circulating flow rate of the liquid nitrogen in the storage box body 1 is improved, the storage box body 1 is kept in a stable low-temperature environment, and the storage effect of the umbilical cord mesenchymal stem cells is enhanced.
An operation method suitable for umbilical cord mesenchymal stem cell storage equipment is characterized by comprising the following steps:
firstly, adjusting the positions of the bearing plates 5 on the rotating shaft 3 through the mounting rings 28 and the mounting bolts 31 according to the length of the test tubes 7, enabling the distance between the upper bearing plate 5 and the lower bearing plate 5 to be suitable for the height of the test tubes 7, selecting the bearing plate 6 with the corresponding bearing hole 34 according to the size of the test tubes 7, and checking whether the circuit of the umbilical cord mesenchymal stem cell storage equipment is complete;
secondly, closing the sealing door 2, opening the ultraviolet lamp 12, performing ultraviolet irradiation sterilization on the storage box body 1, after sterilization, opening the sealing door 2, inserting the test tube 7 filled with the umbilical cord mesenchymal stem cells into the bearing hole 34, placing the bearing disc 6 into the bearing groove 29, and closing the sealing door 2;
thirdly, injecting liquid nitrogen into the liquid nitrogen box 10, starting the liquid nitrogen pump 16, enabling the liquid nitrogen pump 16 to generate negative pressure in the hollow rotating shaft 3 through the liquid suction pipe 17, enabling the liquid nitrogen in the liquid nitrogen box 10 to enter the liquid suction pipe 17 through the liquid suction barrel 24, conveying the liquid nitrogen into the liquid suction chamber 19 from the liquid conveying pipe 18, spraying the liquid nitrogen out of the nozzle 20, and spraying the liquid nitrogen onto the bearing plate 6 in the bearing groove 29 on the bearing plate 5;
fourthly, starting the motor 4, driving the transmission shaft 26 and the driving gear 27 on the transmission shaft 26 to rotate by the motor 4, driving the driven gear 23 to rotate by the driving gear 27, driving the rotating shaft 3 and the bearing plate 5 arranged on the rotating shaft 3 to rotate by the driven gear 23, driving the test tube 7 to rotate by the bearing plate 6, and fully contacting the test tube 7 with the liquid nitrogen sprayed out by the nozzle 20;
fifthly, before taking out the umbilical cord mesenchymal stem cells, the motor 4 and the liquid nitrogen pump 16 are closed, the sealing door 2 is opened, the bearing disc 6 is quickly taken out from the bearing groove 29, and the sealing door 2 is quickly closed after the bearing disc 6 is taken out.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
The parts not involved in the present invention are the same as or can be implemented using the prior art.
Claims (10)
1. The utility model provides an umbilical cord mesenchymal stem cell storage facilities, includes storage box, sealing door, rotation axis, motor, loading board, bears dish and test tube, its characterized in that: the storage box body is arranged on the bracket, a clapboard with a transverse structure is arranged in the storage box body, a liquid nitrogen box is arranged at the bottom of the storage box body, a bearing is arranged on the clapboard, the sealing door is connected with the storage box body through a hinge, the rotating shaft vertically penetrates through the bearing, a driven gear is arranged at the top of the rotating shaft, the motor is arranged at the top outside the storage box body, a transmission shaft is arranged on the motor and vertically extends into the storage box body, a driving gear is arranged on the transmission shaft and is connected with the driving gear and the driven gear, a mounting ring is arranged on the bearing plate, a bearing groove is arranged on the bearing plate, the mounting ring is sleeved on the rotating shaft through a mounting bolt, the bearing plate is movably arranged in the bearing groove and is provided with a bearing hole, the test tube vertically penetrates into the bearing hole, and a sealing plug is arranged at the top of the test tube.
2. The umbilical cord mesenchymal stem cell storage device of claim 1, wherein: all be provided with the heat preservation in bin lateral wall, top and the baffle, be provided with the lamp stand on the storage box inner wall of baffle below, be provided with ultraviolet lamp between lamp stand and lamp stand to be provided with the safety cover of parcel ultraviolet lamp on storing the box inner wall.
3. The umbilical cord mesenchymal stem cell storage device of claim 1, wherein: and a sealing strip is arranged on the sealing door.
4. The umbilical cord mesenchymal stem cell storage device of claim 1 or 2, wherein: the rotating shaft is of a hollow structure, a liquid spraying chamber is arranged on the lower side of the partition plate, a nozzle is arranged on the liquid spraying chamber, a liquid nitrogen pump is arranged at the top in the storage box, a liquid suction pipe and a liquid conveying pipe are arranged on the liquid nitrogen pump, the liquid suction pipe extends into the hollow rotating shaft from the top end of the rotating shaft, and the liquid conveying pipe is connected with the liquid spraying chamber.
5. The umbilical cord mesenchymal stem cell storage device of claim 4, wherein: the bottom end of the rotating shaft is connected with a liquid suction barrel through threads, the liquid suction barrel vertically extends into the liquid nitrogen box, and a liquid suction hole is formed in the side wall of the bottom of the liquid suction barrel.
6. The umbilical cord mesenchymal stem cell storage device of claim 4, wherein: and a sealing ring is arranged between the pipette and the inner wall of the hollow rotating shaft.
7. The umbilical cord mesenchymal stem cell storage device of claim 1, wherein: the bearing plate is arranged into a structure capable of adjusting the position on the rotating shaft through the mounting ring and the mounting bolt, the bearing plate is provided with through holes, and the bearing groove is internally provided with a stop block.
8. The umbilical cord mesenchymal stem cell storage device of claim 1 or 7, wherein: the bearing plate is provided with a handle and is set to be a structure which can be taken out from the bearing groove.
9. The umbilical cord mesenchymal stem cell storage device of claim 8, wherein: and the inner wall of the bearing hole on the bearing plate is provided with an anti-slip pad.
10. An operating method suitable for the umbilical cord mesenchymal stem cell storage device according to any one of claims 1 to 9, wherein the operating method comprises the following steps:
firstly, adjusting the positions of the bearing plates on the rotating shaft through the mounting rings and the mounting bolts according to the length of the test tubes to enable the distance between the upper bearing plate and the lower bearing plate to be adaptive to the height of the test tubes, selecting the bearing plate with the corresponding bearing hole according to the size of the test tubes, and checking whether the line of the umbilical cord mesenchymal stem cell storage equipment is complete;
secondly, closing the sealing door, opening an ultraviolet lamp, carrying out ultraviolet irradiation sterilization on the storage box body, opening the sealing door after sterilization, inserting the test tube filled with the umbilical cord mesenchymal stem cells into the bearing hole, putting the bearing disc into the bearing groove, and closing the sealing door;
injecting liquid nitrogen into the liquid nitrogen box, starting a liquid nitrogen pump, enabling the liquid nitrogen pump to generate negative pressure in the hollow rotating shaft through a liquid suction pipe, enabling the liquid nitrogen in the liquid nitrogen box to enter the liquid suction pipe through a liquid suction cylinder, conveying the liquid nitrogen into a liquid spraying chamber from a liquid conveying pipe, spraying the liquid nitrogen from a nozzle, and spraying the liquid nitrogen onto a bearing disc in a bearing groove on the bearing plate;
fourthly, starting a motor, driving a transmission shaft and a driving gear on the transmission shaft to rotate by the motor, driving a driven gear to rotate by the driving gear, driving a rotating shaft and a bearing plate arranged on the rotating shaft to rotate by the driven gear, driving a test tube to rotate by a bearing plate, and fully contacting the test tube with liquid nitrogen sprayed out from a nozzle;
and fifthly, before taking out the umbilical cord mesenchymal stem cells, closing the motor and the liquid nitrogen pump, opening the sealing door, quickly taking out the bearing disc from the bearing groove, and quickly closing the sealing door after taking out the bearing disc.
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