CN114350534A - Saccharomyces cerevisiae, biological feed for fattening sheep and preparation method and application of biological feed - Google Patents
Saccharomyces cerevisiae, biological feed for fattening sheep and preparation method and application of biological feed Download PDFInfo
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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Abstract
The invention provides saccharomyces cerevisiae, sheep fattening biological feed and a preparation method and application thereof, and relates to the field of animal feed. The Saccharomyces cerevisiae provided by the invention is specifically Saccharomyces cerevisiae (Saccharomyces cerevisiae) FMSW-1, and the preservation number is CGMCC No. 23637. The invention also provides application of the saccharomyces cerevisiae in preparation of biological feeds for fattening sheep. The saccharomyces cerevisiae FMSW-1 can improve the digestion, absorption and utilization rate of biological feed for fattening sheep and improve the nutritional value of the feed by metabolically decomposing and fermenting indigestible macromolecular substances in the bottom materials. By selecting reasonable fermentation substrate materials and compounding and fermenting the saccharomyces cerevisiae FMSW-1 and the pediococcus acidilactici, the daily gain of animals can be increased, the feed intake is increased, the material-weight ratio is reduced, and meanwhile, the intestinal flora of the animals can be improved, and the immunity of organisms can be improved.
Description
Technical Field
The invention relates to the field of animal feed, in particular to saccharomyces cerevisiae, sheep fattening biological feed and a preparation method and application thereof.
Background
The biological feed refers to feed raw materials and additives allowed by relevant national regulations such as feed raw material catalog (2013) and feed additive catalog (2013), and is a general name of feed products developed by biological engineering technologies such as fermentation engineering, enzyme engineering, protein engineering and genetic engineering. The biological feed mainly has the functions of changing the composition of gastrointestinal microbiota, enabling beneficial or harmless microorganisms to occupy population dominance, and regulating intestinal microbial ecological balance by competitively inhibiting the proliferation of pathogenic or harmful microorganisms. Biological feeds are currently widely used in the practice of breeding many types of animals based on their positive role in regulating the intestinal microbial flora of the animal.
The biological feed for fattening sheep, as a green biological feed in the nonreactive breeding era, is applied and paid attention to by masses of farmers and feed enterprises. The biological feed for fattening sheep can improve the fermentation environment of the rumen of sheep and regulate the balance of dominant flora in the digestive tract and microorganisms in the organism; the digestion and utilization efficiency of the feed can be improved, and the production performance and the product quality of the mutton sheep can be improved; can reduce the conversion of protein to ammonia and amine, reduce the foul smell of excrement and improve the environmental sanitation; can also improve the immunologic function in the intestinal tract of the sheep, kill invasive germs in time and enhance the immunity of the sheep to diseases.
In the prior art, biological feeds for fattening sheep are mainly divided into three types according to different feed additives: the fermented feed is produced by feed raw materials and microorganisms through a fermentation engineering technology, the enzymolysis feed is produced by the feed raw materials and an enzyme preparation through an enzyme engineering technology, and the bacterial enzyme synergistic fermented feed is produced by the feed raw materials, the enzyme preparation and the microorganisms through the synergistic effect of the fermentation engineering and the enzyme engineering technology. The fermented feed is prepared by adding microorganisms for fermentation, although beneficial flora for regulating intestinal tracts is introduced, the raw materials of the common feed used for fattening sheep feed are mostly fiber coarse feed, so that the content of the coarse fiber of the prepared fermented feed is high, the digestion and absorption of fattening sheep on nutrient substances in the feed are influenced, and the growth performance and slaughtering performance of the fattening sheep are reduced. The enzymolysis feed can carry out enzymolysis on substances which are difficult to digest and decompose in the feed by adding the enzyme preparation, promotes the digestion and utilization of nutrient substances in the feed, but is lack of the function of regulating intestinal microbial flora. The bacterial enzyme synergistic fermented feed can give consideration to both enzymolysis and improving intestinal flora, but various feed additives are required to be added, which undoubtedly increases the cost of the biological feed for fattening sheep.
The fermentation strain which can improve the nutrient content of the biological feed for fattening sheep and improve the intestinal flora of animals at the same time is provided, and has important practical significance for developing high-quality fermented feed for fattening sheep.
Disclosure of Invention
The invention aims to provide a novel saccharomyces cerevisiae which can improve the content of nutrient substances of biological feed of fattening sheep and improve the intestinal flora of animals, thereby improving the production performance of the animals and the immunity of the organisms of the animals.
In order to achieve the purpose, the invention provides the following technical scheme:
in a first aspect, the present invention provides Saccharomyces cerevisiae FMSW-1, which is deposited in the common microorganism center of the china committee for culture collection of microorganisms at 21/10/2021, wherein the address of the deposition unit: no. 3 Xilu No. 1 Beijing, Chaoyang, and the preservation number is CGMCC No. 23637.
The saccharomyces cerevisiae FMSW-1 provided by the invention is derived from a self-brewing wine collection sample, and the collection method comprises the following steps: picking ripe Amur grape (planted in Shenyang Fengmei biotechnology Co., Ltd.), placing into a sealed tank, adding 20% white sugar by mass of the Amur grape, performing sealed fermentation at 32 ℃ for 50 days, wherein bubbles are generated in the sealed tank and the wine has mellow fragrance, inoculating a proper amount of wine liquid, performing selective separation culture in a wort agar culture medium (130 g/L of malt extract powder, 0.1g/L of chloramphenicol, 40g/L of agar, the balance of water and pH value of 5.6), culturing at 37 ℃ for 48h, growing milky, round and smooth-surfaced slightly-bulged bacterial colonies on the surface of the culture medium, selecting large and full single bacterial colonies, performing subculture in the wort agar culture medium, transferring to 3-4 generations to obtain a single bacterial strain, namely Saccharomyces cerevisiae FMSW-1, and observing by a microscope, wherein the single bacterial strain is shown in figure 1.
In a second aspect, the invention provides application of the saccharomyces cerevisiae in preparation of biological feeds for fattening sheep.
In a third aspect, the invention provides biological feed for fattening sheep, which comprises the saccharomyces cerevisiae.
Further, the biological feed for fattening sheep comprises raw materials of a fermentation substrate, water and a composite bacterial liquid, wherein the composite bacterial liquid comprises a saccharomyces cerevisiae bacterial liquid and a pediococcus acidilactici bacterial liquid, and the saccharomyces cerevisiae bacterial liquid is the saccharomyces cerevisiae bacterial liquid in claim 1.
Further, in the pediococcus acidilactici bacterial liquid, the preservation number of the pediococcus acidilactici is CGMCC No. 20658.
Pediococcus acidilacticiii (Pediococcus acidilacticiii) is obtained from manual pickled Chinese cabbage workshop collected samples, is preserved in China general microbiological culture Collection center (CGMCC) at 9 and 16 days 2020, and the preservation unit address is as follows: no. 3 Xilu No. 1 Beijing, Chaoyang, and the preservation number is CGMCC No. 20658. The strain is disclosed in patent document with application number 202110416893.8 entitled "a high digestibility pediococcus acidilactici fermented wet soybean meal and a preparation method thereof".
Further, the raw materials comprise the following components in parts by weight: 70-80 parts of fermentation bottom materials, 15-25 parts of water and 5-15 parts of compound bacterial liquid,
further, the raw materials comprise the following components in parts by weight: 70 parts of fermentation bottom materials, 20 parts of water and 10 parts of compound bacterial liquid.
Further, the raw materials comprise the following components in parts by weight: 70 parts of fermentation bottom materials, 25 parts of water and 5 parts of compound bacterial liquid.
Further, the raw materials comprise the following components in parts by weight: 70 parts of fermentation bottom materials, 15 parts of water and 15 parts of compound bacterial liquid.
Further, the mass ratio of the saccharomyces cerevisiae bacterial liquid to the pediococcus acidilactici bacterial liquid is 5-7: 3 to 5.
Furthermore, the viable count of the saccharomyces cerevisiae bacterial liquid and the pediococcus acidilactici bacterial liquid is 1.0 multiplied by 109～1.0×1010CFU/mL。
Further, the preparation method of the saccharomyces cerevisiae bacterial liquid comprises the following steps: inoculating the saccharomyces cerevisiae into a malt juice liquid culture medium according to the inoculation amount of 1-10%, culturing for 24-72 h at 32-38 ℃, and activating for 2-3 generations.
Further, the preparation method of the saccharomyces cerevisiae bacterial liquid comprises the following steps: inoculating the saccharomyces cerevisiae into a malt wort liquid culture medium according to the inoculation amount of 1%, culturing for 24h at 37 ℃, and activating for 3 generations.
Further, the preparation method of the pediococcus acidilactici bacterial liquid comprises the following steps: inoculating the pediococcus acidilactici into an MRS liquid culture medium according to the inoculation amount of 1-10%, culturing at 32-38 ℃ for 24-72 h, and activating for 2-3 generations.
Further, the preparation method of the pediococcus acidilactici bacterial liquid comprises the following steps: inoculating the pediococcus acidilactici into an MRS liquid culture medium according to the inoculation amount of 1%, culturing at 37 ℃ for 24h, and activating for 3 generations.
Further, the wort liquid medium consists of the following components: 130g/L of malt extract powder, 0.1g/L of chloramphenicol and the balance of water, and the pH value is 5.4-5.8.
Further, the MRS liquid culture medium consists of the following components: 10.0g/L of casein digest, 2.0g/L of triammonium citrate, 10.0g/L of beef extract powder, 0.2g/L of magnesium sulfate, 4.0g/L of yeast extract powder, 0.05g/L of manganese sulfate, 5.0g/L of sodium acetate, 2.0g/L of dipotassium hydrogen phosphate, 20.0g/L of glucose, 801.08g/L of tween-801.08, and the balance of water, wherein the pH value is 5.5-5.9.
Further, the fermentation substrate comprises the following components in a mass ratio of 3-5: 2-4: 3-4 of pressed corn, bran and gunite corn bran.
Further, the fermentation substrate comprises the following components in a mass ratio of 4: 3: 3. 3: 4: 4. 5: 2: 3 flaked corn, bran and jet-grouted corn bran in any proportion.
In a fourth aspect, the invention provides a preparation method of the biological feed for fattening sheep, which comprises the following steps: and mixing the fermentation substrate, water and the composite bacterial liquid, and culturing at 32-38 ℃ for 24-96 h.
Further, the fermentation substrate, water and the complex bacterial liquid were mixed and cultured at 37 ℃ for 72 hours.
In a fifth aspect, the invention provides application of the biological feed for fattening sheep or the biological feed for fattening sheep obtained by the preparation method in improving animal production performance and/or improving animal organism immunity.
The technical scheme of the invention has the following advantages:
the Saccharomyces cerevisiae FMSW-1 provided by the invention can improve the digestion, absorption and utilization rate of biological feed for fattening sheep and improve the nutritional value of the feed by metabolically decomposing and fermenting indigestible macromolecular substances in the bottom material. Compared with other saccharomyces cerevisiae, the saccharomyces cerevisiae FMSW-1 can obviously improve the content of viable bacteria, the content of crude protein, the ratio of acid soluble protein and the content of total acid in the feed, and can obviously reduce the content of crude fiber. Compared with biological feeds for fattening sheep prepared from other saccharomyces cerevisiae, the saccharomyces cerevisiae FMSW-1 has the advantages that the content of essential amino acid and the content of flavor amino acid are increased, wherein the essential amino acid is beneficial to improving the production performance of animals, the content of flavor amino acid is increased, the flavor of the feeds is improved, and the food intake of the animals is increased.
According to the biological feed for fattening sheep provided by the invention, reasonable fermentation bottom materials are selected, and saccharomyces cerevisiae FMSW-1 and pediococcus acidilactici are compounded and fermented, so that the daily gain of animals can be increased, the feed intake is increased, the feed-weight ratio is reduced, and the economic benefit can be indirectly improved. Meanwhile, the biological feed for fattening sheep provided by the invention can reduce the number of intestinal harmful bacteria escherichia coli in excrement, increase the number of intestinal probiotic lactic acid bacteria in excrement, improve animal intestinal flora and improve organism immunity.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a photograph of Saccharomyces cerevisiae FMSW-1 according to the present invention observed under a microscope.
Detailed Description
The following examples are provided to further understand the present invention, not to limit the scope of the present invention, but to provide the best mode, not to limit the content and the protection scope of the present invention, and any product similar or similar to the present invention, which is obtained by combining the present invention with other prior art features, falls within the protection scope of the present invention.
The Pediococcus acidilacticiii used in the embodiment of the invention is preserved in the China general microbiological culture Collection center of the Committee for culture Collection of microorganisms with the preservation number of CGMCC No. 20658.
The malt wort liquid culture medium in the embodiment of the invention consists of the following components: 130g/L of malt extract powder, 0.1g/L of chloramphenicol and the balance of water, and the pH value is 5.4-5.8.
The MRS liquid culture medium in the embodiment of the invention comprises the following components: 10.0g/L of casein digest, 2.0g/L of triammonium citrate, 10.0g/L of beef extract powder, 0.2g/L of magnesium sulfate, 4.0g/L of yeast extract powder, 0.05g/L of manganese sulfate, 5.0g/L of sodium acetate, 2.0g/L of dipotassium hydrogen phosphate, 20.0g/L of glucose, 801.08g/L of tween-801.08, and the balance of water, wherein the pH value is 5.5-5.9.
The examples do not show the specific experimental steps or conditions, and can be performed according to the conventional experimental steps described in the literature in the field. The raw materials or equipment used are all conventional products which can be obtained commercially, including but not limited to the raw materials or equipment used in the examples of the present application.
Example 1
The embodiment provides a biological feed for fattening sheep, which is prepared by the following steps:
(1) preparation of composite bacterial liquid
Inoculating Saccharomyces cerevisiae FMSW-1 into wort liquid culture medium according to 1% inoculation amount, culturing at 37 deg.C for 24 hr for activation, inoculating the activated Saccharomyces cerevisiae seed liquid into wort liquid culture medium according to 1% inoculation amount, culturing at 37 deg.C for 24 hr, co-activating for 3 generations according to the above steps, and detecting viable count up to 109CFU/mL to obtain saccharomyces cerevisiae bacterial liquid;
inoculating Pediococcus acidilactici to MRS liquid culture medium according to the inoculation amount of 1%, culturing at 37 ℃ for 24h for activation, inoculating the activated Pediococcus acidilactici seed liquid to the MRS liquid culture medium according to the inoculation amount of 1%, culturing at 37 ℃ for 24h, activating for 3 generations according to the steps, and detecting that the viable count can reach 109CFU/mL to obtain pediococcus acidilactici bacterial liquid;
mixing the saccharomyces cerevisiae bacterial liquid and the pediococcus acidilactici bacterial liquid according to the mass ratio of 1:1, mixing to obtain a compound bacterial liquid.
(2) Preparation of fermentation substrate
Crushing the guniting corn bran and the bran, wherein the tabletting corn is not crushed, and the mass ratio of the tabletting corn to the bran to the guniting corn bran is 4: 3: 3, uniformly mixing to obtain the fermentation substrate.
(3) Fermentation of
And mixing the prepared fermentation substrate and the compound bacterial liquid according to 70% of the fermentation substrate, 20% of water and 10% of the compound bacterial liquid, and fermenting at the constant temperature of 37 ℃ for 72 hours to obtain the biological feed for fattening sheep.
Example 2
The present example provides a biological feed for fattening sheep, the preparation method of which is as described in example 1, except that, in step (3), 70% of fermentation substrate, 25% of water, and 5% of compound bacterial liquid are mixed.
Example 3
The present embodiment provides a biological feed for fattening sheep, which is prepared according to the method of embodiment 1, except that, in the step (3), 70% of fermentation substrate, 5% of water, and 15% of compound bacterial liquid are mixed.
Example 4
The embodiment provides a biological feed for fattening sheep, which is prepared according to the preparation method of the biological feed in the embodiment 1, and is characterized in that in the step (2), the mass ratio of the pressed corn to the bran to the gunite corn bran is 3: 4: 4.
example 5
The embodiment provides a biological feed for fattening sheep, which is prepared according to the preparation method of the biological feed in the embodiment 1, and is characterized in that in the step (2), the mass ratio of the pressed corn to the bran to the gunite corn bran is 5: 2: 3.
comparative example 1
The comparative example provides a biological feed for fattening sheep, and the preparation method thereof refers to example 1, and only differs in that the bacterial liquid of Angel saccharomyces cerevisiae is used to replace the saccharomyces cerevisiae bacterial liquid in example 1.
Angel saccharomyces cerevisiae is high-temperature-resistant high-activity dry saccharomyces cerevisiae, and is purchased from Angel saccharomyces cerevisiae GmbH, and is in milk white granules. Weighing 1g of the mixture to be dissolved in 100mL of sterilized waterIn the method, 100uL of the strain is taken to be coated on a malt extract agar culture medium (130 g/L of malt extract powder, 0.1g/L of chloramphenicol, 40g/L of agar, the balance of water and the pH value of 5.4-5.8) for selective separation culture, after 48 hours of culture at 37 ℃, milky, round and smooth-surfaced colony grows on the surface of the culture medium, then a single colony with large and full colony is selected to be subcultured in the malt extract agar culture medium, a single strain is obtained after 3-4 generations, the inoculation amount of the single colony is selected to be inoculated into the malt extract liquid culture medium, the single colony is cultured at 37 ℃ for 24 hours for activation, then the activated saccharomyces cerevisiae seed liquid is inoculated into the malt extract liquid culture medium according to 1% of the inoculation amount, the saccharomyces cerevisiae culture liquid is cultured at 37 ℃ for 24 hours, the combined activation is carried out for 3 generations according to the steps, and the number of viable bacteria can reach 109And CFU/mL to obtain the bacterial liquid of Angel saccharomyces cerevisiae.
Comparative example 2
The comparative example provides a biological feed for fattening sheep, and the preparation method thereof refers to example 1, and only differs in that the saccharomyces cerevisiae bacterial liquid in example 1 is replaced by the bacterial liquid of Guangzhou micro-element saccharomyces cerevisiae.
Guangzhou micro saccharomyces cerevisiae purchased from micro-biological science and technology Limited, Guangzhou, yeast powder, active dry yeast for feeding and milk gray particles. Weighing 1g of the mixture, dissolving the mixture in 100mL of sterilized water, coating 100uL of the mixture on a malt extract agar culture medium (130 g/L of malt extract powder, 0.1g/L of chloramphenicol, 40g/L of agar and the balance of water, the pH value is 5.4-5.8) for selective separation culture, culturing at 37 ℃ for 48 hours to ensure that the surface of the culture medium grows milky and round, the method comprises the following steps of selecting a colony with a smooth and slightly bulged surface, selecting a single plump colony which is large in colony size to perform subculture in a malt wort agar culture medium, transferring to 3-4 generations to obtain a single strain, selecting the inoculation amount of the single colony to inoculate in the malt wort culture medium, culturing at 37 ℃ for 24h to activate, inoculating the activated saccharomyces cerevisiae seed liquid into the malt wort culture medium according to 1% of the inoculation amount, culturing at 37 ℃ for 24h, co-activating for 3 generations according to the steps, and detecting that the number of viable bacteria can reach 10.9CFU/mL to obtain the bacterial liquid of Guangzhou micro saccharomyces cerevisiae.
Experimental example 1
The experimental example aims to carry out nutrition index detection on the fattening sheep biological feed prepared in the examples 1-5 and the comparative examples 1-2, and comprises the following steps: total viable count, crude protein content, acid soluble protein ratio, crude ash content, total acid content, crude fiber content, and 17 amino acid content. The detection method of each index is as follows:
the method for detecting the total number of the viable bacteria comprises the following steps:
weighing 25g of sample by aseptic technique, placing the sample in an aseptic homogenizing cup filled with 225mL of physiological saline, and homogenizing at 10000r/min for 2min to obtain 1:10 sample homogenizing solution; sucking 1:10 sample homogenizing solution 1mL by using a 1mL sterile sucker or a micropipette, slowly injecting the sample homogenizing solution into a sterile test tube filled with 9mL physiological saline along the tube wall, and fully and uniformly mixing by vortex oscillation to prepare the sample homogenizing solution 1: 100. Taking another 1mL sterile pipette or micropipette suction head, according to the above operation sequence, making 10 times of progressively increased sample homogenizing liquid, and changing 1mL sterile pipette or suction head for every time of progressively increased dilution. According to the estimation of the total viable count of the sample to be detected, 3 continuous suitable dilutions are selected, 0.1mL of sample homogenate is absorbed by each dilution and is respectively placed on 2 agar plates, and an L-shaped rod is used for surface coating. The plates were inverted and incubated in an incubator at 30. + -. 1 ℃ for 48h, and the number of all colonies on the plates was counted. The dilution of the sample to plate coating was completed in 30 min.
And (4) calculating a result:
the viable bacteria content of the product is the total viable bacteria number obtained by detection.
Reports are made from colony counts, weighing samples are reported in CFU/g, volume samples are reported in CFU/mL.
The detection method of the content of the crude protein refers to a Kjeldahl method for determining the crude protein in the feed GB/T6432-2018;
the acid soluble protein ratio detection method refers to the determination of acid soluble protein in NY/T3801-;
the detection method of the crude ash refers to the measurement of the crude ash in GB/T6438-2007 feeds;
the detection method of the total acid content refers to the determination of the total acid in the national standard food for the food safety of GB 12456-;
the detection method of the content of the crude fiber refers to a content determination-filtration method of the crude fiber in the feed GB/T6434-2006;
the detection method of the amino acid content refers to a colorimetric method for determining amino acid nitrogen in food according to the national standard of GB5009.235-2016 food safety.
As a control, the nutritional indicators of the fermentation substrate prepared in step (2) of example 1 were also examined according to the method described above.
The results of the tests of examples 1 and comparative examples 1 to 2 are shown in Table 1, the results of the tests of examples 1 to 3 are shown in Table 2, and the results of the tests of examples 1 and 4 to 6 are shown in Table 3.
TABLE 1 comparison of test results of example 1 and comparative examples 1 to 2
As shown in Table 1, the experimental result shows that the raw protein content of the saccharomyces cerevisiae FMSW-1 prepared by fermentation is increased by 32.48%, the acid soluble protein content is increased by 32.9%, the total acid content is increased by 81.4%, and the crude fiber content is reduced by 2% compared with the raw protein content of a fermentation substrate before fermentation; compared with the method for preparing the biological feed for fattening sheep by fermenting Angel saccharomyces cerevisiae and Guangzhou micro saccharomyces cerevisiae in the prior art, the method for preparing the biological feed for fattening sheep by fermenting the saccharomyces cerevisiae FMSW-1 provided by the invention obviously improves the total viable count, the crude protein content, the acid soluble protein ratio and the total acid content of the feed, improves the content of each amino acid and obviously reduces the crude fiber content of the feed. The saccharomyces cerevisiae FMSW-1 is fermented to prepare the biological feed for fattening sheep, the content of essential amino acid and the content of flavor amino acid are both improved, the essential amino acid is beneficial to improving the production performance of animals, and the flavor amino acid is beneficial to improving the flavor of the feed, so that the food intake of the animals is increased.
TABLE 2 comparison of test results of examples 1 to 3
As shown in Table 2, the experimental results show that the addition of different proportions of composite bacterial liquid for fermentation has an influence on the nutritional indexes of the biological feed for fattening sheep, the optimization rate of the nutritional indexes is higher than that of the fermentation of 5% and 15% of composite bacterial liquid after the treatment of 10% of composite bacterial liquid, and although the inoculation fermentation effect of part of 15% of composite bacterial liquid is also good, the inoculation of 10% of composite bacterial liquid is most suitable for cost consideration.
TABLE 3 comparison of test results of examples 1, 4 to 5
As shown in table 3, experimental results show that the fermented feeds for fattening sheep prepared by adopting fermentation base materials with different substrate ratios have different nutritional indexes, and the mass ratio of the pressed corn to the bran to the gunite corn bran is 4: 3: 3, the feed is most abundant in nutrition, can meet the nutritional requirements of animals, improves the immunity of organisms, has the highest amino acid component content and higher flavor amino acid content, and is beneficial to improving the palatability of animals.
Experimental example 2
The experimental example aims to research the influence of the biological feed for fattening sheep on the production performance and intestinal flora of the fattening sheep.
The experiment is carried out in a fattening sheep stage, 150 fattening sheep (Qianlin Taoisun county) with the weight of about 30 kg are randomly divided into 3 groups, each group is divided into 5 repeated groups, each repeated group comprises 10 sheep, namely a control group, an experiment group 1 and an experiment group 2, and each group is fed in the following mode:
control group: feeding fattening sheep daily ration.
Experimental group 1: the biological feed for fattening sheep prepared in example 5 is added into the daily ration for fattening sheep, and the addition amount of the biological feed for fattening sheep is 10% of the daily ration for fattening sheep.
Experimental group 2: the biological feed for fattening sheep prepared in example 5 is added into the daily ration for fattening sheep, and the addition amount of the biological feed for fattening sheep is 20% of the daily ration for fattening sheep.
Feeding fattening sheep daily ration and fattening sheep biological feed for 3 kilograms each day, feeding for three times, feeding for one kilogram each time, wherein the feeding is 8 days earlier: 00. at noon 12: 00. and 5, at night: 00; and after feeding, the food is taken for 2 to 3 hours freely, and then the food intake is recorded. The experimental period was 30 days. The composition and the nutrient content of the fattening sheep ration are shown in the table 4-5.
TABLE 4 sheep fattening daily ration composition
| Raw materials | Ratio (%) |
| Corn (corn) | 44 |
| Corn stalk | 17.5 |
| DDGS | 24 |
| Bean pulp (43) | 5 |
| Stone powder | 1 |
| Ammonium chloride | 0.5 |
| Salt | 0.5 |
| Baking soda | 0.5 |
| Soybean coat | 7 |
| Total up to | 100 |
TABLE 5 fattening sheep ration Nutrition content
| Nutritional index | Nutrient content |
| Digestive energy MJ/Kg | 90.13 |
| Ash content% | 6.2 |
| Crude protein% | 14.95 |
| Crude fat% | 5.10 |
| Neutral detergent fiber% | 29.90 |
| Acid detergent fiber% | 15.25 |
| Lignin% | 3.5 |
| Calcium content% | 0.5 |
| Phosphorus% | 0.4 |
The production performance indexes of the fattening sheep of the control group and the experimental group 1-2 are counted, SPSS 9.1(2003) software is adopted to carry out One-way ANOVA (One-way ANOVA), the result is represented by 'mean value plus or minus standard deviation', the multiple comparison selection method is LSD, P is less than 0.01 (difference is extremely significant), P is less than 0.05 (difference is significant) and is used as the judgment standard of the difference significance, and the result is shown in Table 6.
TABLE 6 results of production performance indexes of fattening sheep of control group and experimental group 1-2
Note: the same lower case letters of the shoulder marks of the same row data indicate that the difference is not significant (P >0.05), the adjacent lower case letters of the shoulder marks indicate that the difference is significant (P <0.05), and the lower case letters of the shoulder marks of the same row data indicate that the difference is significant (P < 0.01).
As shown in Table 6, the biological feed for fattening sheep can improve the daily gain, increase the feed intake and reduce the feed-weight ratio to different degrees, wherein when the addition amount is 20%, the feed-weight ratio is reduced most, and the economic benefit can be indirectly improved.
And (3) detecting the quantity of escherichia coli and lactic acid bacteria in the excrement of the fattening sheep after the experiment is finished, wherein the detection method refers to a total viable bacteria detection method, the results of each group are expressed in the form of an intra-group mean value, and the results are shown in table 7.
TABLE 7 detection results of sheep fattening excrement microorganisms of control group and experimental group 1-2
| Group of | Control group | Experimental group 1 | Experimental group 2 |
| Escherichia coli (cfu/g) | 2.13×107 | 5.02×105 | 1.87×104 |
| Lactic acid bacteria (cfu/g) | 5.47×105 | 6.88×107 | 4.01×108 |
As shown in Table 7, the addition of the biological feed for fattening sheep can reduce the number of harmful intestinal bacteria Escherichia coli in feces and increase the number of probiotic lactic acid bacteria in feces, and the health condition of intestinal flora can be indirectly expressed by the microbial condition of feces, which indicates that the biological feed for fattening sheep can obviously improve the intestinal flora of animals and improve the immunity of organisms.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (10)
1. The Saccharomyces cerevisiae is specifically Saccharomyces cerevisiae (Saccharomyces cerevisiae) FMSW-1 with the preservation number of CGMCC No. 23637.
2. The use of saccharomyces cerevisiae as claimed in claim 1 for the preparation of biological feed for fattening sheep.
3. A biological feed for fattening sheep, characterized by comprising the Saccharomyces cerevisiae of claim 1.
4. The biological feed for fattening sheep according to claim 3, wherein the raw materials comprise a fermentation substrate, water and a composite bacterial liquid, the composite bacterial liquid comprises a saccharomyces cerevisiae bacterial liquid and a pediococcus acidilactici bacterial liquid, and the saccharomyces cerevisiae bacterial liquid is the saccharomyces cerevisiae bacterial liquid according to claim 1.
5. The biological feed for fattening sheep according to claim 4, wherein the raw materials comprise the following components in parts by weight: 70-80 parts of fermentation bottom materials, 15-25 parts of water and 5-15 parts of compound bacterial liquid,
preferably, the raw materials comprise the following components:
70 parts of fermentation bottom material, 20 parts of water and 10 parts of compound bacterial liquid, or
70 parts of fermentation bottom material, 25 parts of water and 5 parts of compound bacterial liquid, or
70 parts of fermentation bottom materials, 15 parts of water and 15 parts of compound bacterial liquid.
6. The biological feed for fattening sheep according to claim 4,
in the pediococcus acidilactici bacterial liquid, the preservation number of the pediococcus acidilactici is CGMCC No. 20658;
the mass ratio of the saccharomyces cerevisiae bacterial liquid to the pediococcus acidilactici bacterial liquid is 5-7: 3-5;
the viable count of the saccharomyces cerevisiae bacterial liquid and the pediococcus acidilactici bacterial liquid is 1.0 multiplied by 109～1.0×1010CFU/mL。
7. The biological feed for fattening sheep according to claim 4,
the preparation method of the saccharomyces cerevisiae bacterial liquid comprises the following steps: inoculating the saccharomyces cerevisiae into a malt juice liquid culture medium according to the inoculation amount of 1-10%, culturing for 24-72 h at 32-38 ℃, and activating for 2-3 generations;
the malt wort liquid culture medium consists of the following components: 130g/L of malt extract powder, 0.1g/L of chloramphenicol and the balance of water, wherein the pH value is 5.4-5.8;
the preparation method of the pediococcus acidilactici bacterial liquid comprises the following steps: inoculating the pediococcus acidilactici into an MRS liquid culture medium according to the inoculation amount of 1-10%, culturing at 32-38 ℃ for 24-72 h, and activating for 2-3 generations;
the MRS liquid culture medium consists of the following components: 10.0g/L of casein digest, 2.0g/L of triammonium citrate, 10.0g/L of beef extract powder, 0.2g/L of magnesium sulfate, 4.0g/L of yeast extract powder, 0.05g/L of manganese sulfate, 5.0g/L of sodium acetate, 2.0g/L of dipotassium hydrogen phosphate, 20.0g/L of glucose, 801.08g/L of tween-801.08, and the balance of water, wherein the pH value is 5.5-5.9.
8. The biological feed for fattening sheep according to claim 4, wherein the fermentation substrate comprises the following components in a mass ratio of 3-5: 2-4: 3-4 of pressed corn, bran and gunite corn bran,
preferably, the fermentation substrate comprises a mixture of the following components in a mass ratio of 4: 3: 3. 3: 4: 4. 5: 2: 3 flaked corn, bran and jet-grouted corn bran in any proportion.
9. The preparation method of the biological feed for fattening sheep according to any one of claims 4 to 8, characterized by comprising the following steps: and mixing the fermentation substrate, water and the compound bacterial liquid, and then culturing for 24-96 h at 32-38 ℃, preferably, mixing the fermentation substrate, the water and the compound bacterial liquid, and then culturing for 72h at 37 ℃.
10. Use of the biological feed for fattening sheep according to any one of claims 3 to 8 or the biological feed for fattening sheep obtained by the preparation method according to claim 9 for improving animal productivity and/or enhancing animal immunity.
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| CN114350534B (en) | 2023-12-29 |
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