CN114317662A - Preparation method of triketone dehydrogenated substance - Google Patents
Preparation method of triketone dehydrogenated substance Download PDFInfo
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- CN114317662A CN114317662A CN202210013661.2A CN202210013661A CN114317662A CN 114317662 A CN114317662 A CN 114317662A CN 202210013661 A CN202210013661 A CN 202210013661A CN 114317662 A CN114317662 A CN 114317662A
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- 238000006243 chemical reaction Methods 0.000 claims abstract description 92
- VOYADQIFGGIKAT-UHFFFAOYSA-N 1,3-dibutyl-4-hydroxy-2,6-dioxopyrimidine-5-carboximidamide Chemical compound CCCCn1c(O)c(C(N)=N)c(=O)n(CCCC)c1=O VOYADQIFGGIKAT-UHFFFAOYSA-N 0.000 claims abstract description 46
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- 229910052739 hydrogen Inorganic materials 0.000 claims 1
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Abstract
The invention belongs to the technical field of steroid hormone preparation, and particularly relates to a preparation method of a trione dehydrogenized substance, which comprises the following steps of mixing a microorganism containing 3-sterone-1-dehydrogenase with raw material trione, simultaneously adding a surfactant, a polyether defoamer PPE and an exogenous electron acceptor, and not adding an organic solvent, carrying out microbial fermentation, and obtaining the trione dehydrogenized substance after the fermentation is finished, wherein the reaction route is as follows:
the invention effectively improves the conversion rate of the product and the yield.
Description
Technical Field
The invention belongs to the technical field of preparation of steroid hormones, and particularly relates to a preparation method of a triketone dehydrogenated substance.
Background
The common method for producing steroid medicines is a combined process route of chemical synthesis and microbial transformation, and the key steps of reaction are usually realized by microbial transformation, including dehydrogenation of A ring C1, 2-position. The transformation of steroids by microorganisms is effected by an enzyme or enzyme system in the microorganism, and the enzyme acting for dehydrogenation of steroid C1, 2-position is called 3-sterone-1-dehydrogenase (KSDD), also called steroid C1, 2-position dehydrogenase. The enzyme is usually present in microorganisms such as Nocardia (Nocardia sp.), Rhodococcus (Rhodococcus sp.), Bacillus (Bacillus sp.), Mycobacterium (Mycobacterium sp.), and Arthrobacter (Arthrobacter sp.). Factors influencing the conversion rate of biological dehydrogenation of steroids, in addition to the solubility of the substrate, the most influential factor is the activity of the target microbial enzyme. Therefore, how to improve the enzyme activity is one of the key points of the steroid dehydrogenation reaction.
The trione is an important intermediate product and raw material in the production process of steroid drugs, and the C1, 2-dehydrogenation of the trione can effectively improve the anti-inflammatory activity of subsequent products, and is one of important reactions in the preparation process of steroid compounds. At present, the triketone dehydrogenation is mainly completed by adopting a biotransformation method, but due to the influences of factors such as poor substrate solubility, difficult improvement of microorganism (enzyme) activity and the like, the transformation period is long, the transformation rate is low, the separation and purification difficulty is high, and the yield is difficult to improve.
Disclosure of Invention
The invention aims to provide a preparation method of a triketone dehydrogenated substance, which can effectively improve the conversion rate of products and the yield.
The invention relates to a preparation method of a trione dehydrogenated substance, which comprises the following steps of mixing a microorganism containing 3-sterone-1-dehydrogenase with raw material trione, simultaneously adding a surfactant, a polyether defoamer PPE and an exogenous electron acceptor, and not adding an organic solvent, carrying out microbial fermentation, and obtaining the trione dehydrogenated substance after the fermentation is finished, wherein the reaction route is as follows:
preferably, the microorganism containing 3-sterone-1-dehydrogenase is Arthrobacter simplex (Arthrobacter simplex), preferably, it is Arthrobacter simplex (Arthrobacter simplex) CPCC 140451.
The method for culturing the Arthrobacter simplicissimsii comprises the following steps:
(1) slant culture: the slant culture medium comprises the following substances in percentage by mass volume (w/v): 0.1-2% of glucose, 0.1-2% of yeast extract, 7.0-8.0% of pH, 0.5-2% of agar, sterilizing at 121 ℃ for 30min, and inoculating the Arthrobacter simplex strain. Culturing in a constant temperature incubator at 25-32 deg.C for 1-6 days.
(2) First-order seed culture: the primary seed culture medium comprises the following substances in percentage by mass (w/v): 0.1-2% of glucose, 0.1-2% of corn steep liquor, 0.1-1% of peptone, 0.1-1% of yeast extract, 0.1-1% of potassium dihydrogen phosphate and pH 7.0-8.0. Sterilizing at 121 deg.C for 30 min. Inoculating Arthrobacter simplex slant strains under aseptic conditions, and performing shaking culture on a shaking table at the speed of 150-.
(3) Secondary seed culture: the secondary seed culture medium comprises the following substances in percentage by mass (w/v): 0.1-2% of glucose, 0.1-2% of corn steep liquor, 0.1-1% of peptone, 0.1-1% of yeast extract, 0.1-1% of potassium dihydrogen phosphate and pH 7.0-8.0. Sterilizing at 121 deg.C for 30 min. Inoculating the first-stage Arthrobacter simplex seeds under aseptic conditions, wherein the inoculation amount is 2.5-30%, the shaking culture is carried out for 10-72h at 25-32 ℃ and 50-250rpm on a shaking table, and thus obtaining the second-stage Arthrobacter simplex seed culture solution.
(4) Shake flask transformation
Adding triketone, Tween-80, polyether defoamer PPE and PMS into the secondary seeds with the weight concentration of 0.1-10% of triketone, 0.01-0.5% of Tween-80, 0.1-5% of polyether defoamer PPE and 0.001-0.5% of PMS respectively. The mixture was then transferred by shaking at 25-32 ℃ and 50-250rpm, and samples were taken every 24h for TLC and HPLC analysis.
(5)1 liter jar conversion
Fermentation tank culture medium: the secondary seed culture medium comprises the following substances in percentage by mass (w/v): 0.1-2% of glucose, 0.1-2% of corn steep liquor, 0.1-1% of peptone, 0.1-1% of yeast extract, 0.1-1% of potassium dihydrogen phosphate and pH 7.0-8.0. Sterilizing at 121 deg.C for 30 min. Inoculating 2.5-30% Arthrobacter simplex shake flask seeds under aseptic condition, culturing at 25-32 ℃ and 200-800rpm for 10-72h, uncovering, and adding triketone, Tween-80, polyether defoamer PPE and PMS, wherein the weight concentrations are 0.1-10% triketone crushed material, 0.01-0.5% Tween-80, 0.1-5% polyether defoamer PPE and 0.001-0.5% PMS respectively.
The transformation conditions were: at 25-32 ℃, 200-800rpm, aeration 0.2-0.4m3/h, pot pressure 0.02-0.1MPa, sampling every 24h for TLC and HPLC analysis.
Preferably, the surfactant is tween-80, and the exogenous electron acceptor is an exogenous electron acceptor PMS.
Preferably, the weight ratio of the starting triketone to the polyether defoamer PPE is 3:0.5-2, more preferably 3: 2.
Preferably, in the fermentation system, the weight concentration of the raw material triketone is 0.1-10%, the weight concentration of the surfactant is 0.01-0.5%, the weight concentration of the polyether defoamer PPE is 0.1-5%, and the weight concentration of the exogenous electron acceptor is 0.001-0.5%.
Preferably, the fermentation temperature is 25-32 ℃, the rotation speed is 200-800rpm, and the ventilation volume is 0.2-0.4m3The tank pressure is 0.02-0.1 MPa.
The invention has the beneficial effects that the inventor firstly adds organic solvents such as methanol, ethanol, DMF, DMSO, acetone and the like to improve the solubility of the substrate, the improvement of the solubility of the substrate is generally beneficial to improving the yield of the product, for example, the addition of the organic solvents such as acetone, ethanol and the like can improve the yield of the product, but actually, the yield of the product is reduced after partial organic solvents such as DMSO are added, which shows that the toxic effect of the partial organic solvents on thalli is larger than the cosolvent effect on the substrate.
In order to further improve the solubility of the substrate, the inventors added the surfactant tween-80, and found that tween-80 was advantageous to improve the yield of the product. The inventors have tried different surfactants and found that the addition of other kinds or more of surfactants has a limited effect on the improvement of the product.
According to the application, the surfactant Tween-80 and the polyether defoamer PPE are added, the substrate is crushed and sieved, the dispersity of the substrate is improved, and the substrate is effectively contacted with the enzyme, so that the effect is obvious, particularly, the polyether defoamer PPE can be used as a nonionic surfactant to play roles in solubilization and dispersion besides the defoaming role, and the conversion efficiency of microorganisms (enzymes) can be improved by adding the exogenous electron acceptor/the hydrogen donor Phenazine Methosulfate (PMS). By the means, the practical problems of low conversion rate, long conversion period, difficulty in separation and purification, low yield and the like caused by poor substrate solubility/dispersibility and low thallus (dehydrogenase) activity in the prior triketone dehydrogenation process are effectively solved.
The method takes triketone as a substrate, simple Arthrobacter (Arthrobacter simplex) as a converting enzyme source, crushes and screens the substrate in a conventional converting system, and adds surfactant Tween-80, nonionic surfactant polyether defoamer PPE and exogenous electron receptor PMS, so that the conversion rate can be greatly improved, the conversion time is shortened, the conversion condition is mild, the operation is simple, and the method can be applied to large-scale production.
Drawings
FIG. 1 is a diagram showing the results of HPLC analysis in example 4 of the present invention.
FIG. 2 is a graph showing the results of HPLC analysis in example 5 of the present invention.
Detailed Description
Example 1 Arthrobacter simplex seed culture
Slant culture medium: glucose 2%, yeast extract 2%, pH 7.5, agar 2%, sterilizing at 121 deg.C for 30min, and inoculating Arthrobacter simplex (Arthrobacter simplex) CPCC 140451. The culture conditions are as follows: 25-32 ℃ for 1-6 days.
Primary seed culture medium: 2 percent of glucose, 2 percent of corn steep liquor, 1 percent of peptone, 0.5 percent of yeast extract, 0.5 percent of monopotassium phosphate and pH7.0-8.0. Sterilizing at 121 deg.C for 30 min. Inoculating Arthrobacter simplex slant strains under aseptic conditions, wherein the culture conditions are as follows: 500ml shake flask is filled with 100ml seed culture medium, the temperature is 30-32 ℃, the rotation speed is 200rpm, and the culture time is 48 h.
Secondary seed culture medium: 2 percent of glucose, 2 percent of corn steep liquor, 1 percent of peptone, 0.5 percent of yeast extract, 0.5 percent of monopotassium phosphate and pH7.0-8.0. Sterilizing at 121 deg.C for 30 min. Inoculating the first-class seeds under the aseptic condition, wherein the inoculation amount is 20 percent, and the culture condition is as follows: 100ml of seed culture medium is filled in a 500ml shake flask, the rotation speed is 200rpm, and the culture time is 48h at 30 ℃.
Seed culture medium of fermentation tank: 2 percent of glucose, 2 percent of corn steep liquor, 1 percent of peptone, 0.5 percent of yeast extract, 0.5 percent of monopotassium phosphate and pH7.0-8.0. Sterilizing at 121 deg.C for 30 min. Inoculating the second-stage shake flask seeds under the aseptic condition, wherein the inoculation amount is 20 percent, and the culture condition is as follows: the 10 liters is canned with 6 liters of seed culture medium, the rotating speed is 300rpm at 30 ℃, and the culture time is 40 to 48 hours.
Comparative example 1
Shaking seed culture was performed according to example 1, the primary seed solution was inoculated into 100ml of secondary seed medium for secondary seed culture, and after 48h of culture, 3g of triketone crushed material and 0.5g of PPE (polyoxyethylene polyoxypropylene pentaerythritol ether) as a polyether antifoaming agent were added to the cultured secondary seeds, and the conversion conditions: 30 ℃, 200rpm, the conversion time is 64h, and after the conversion is finished, the HPLC is checked, the conversion rate of the trione dehydrogenation product is 65.15 percent, and the conversion rate of the trione is 33.06 percent.
Comparative example 2
The shake flask seed culture was performed according to example 1, the primary seed solution was inoculated into 100ml of secondary seed medium for secondary seed culture, and after 48h of culture, 3g of triketone ground material, 0.05g of tween-80, 0.5g of PPE, which is a polyether defoamer, were added to the cultured secondary seeds, and the transformation conditions: 30 ℃, 200rpm, the conversion time is 64h, and after the conversion is finished, the HPLC is checked, the conversion rate of the trione dehydrogenation product is 83.58 percent, and the conversion rate of the trione is 15.65 percent.
Comparative example 3
Shake flask seed culture was performed according to example 1, the primary seed solution was inoculated into 100ml of secondary seed medium for secondary seed culture, after 48h of culture, 3g of triketone ground material, 0.05g of tween-80, 0.5g of PMS were added to the cultured secondary seeds, and the transformation conditions: 30 ℃, 200rpm, the conversion time is 64h, and after the conversion is finished, the HPLC is checked, the conversion rate of the trione dehydrogenation product is 38.53 percent, and the conversion rate of the trione is 59.89 percent.
Comparative example 4
Shake flask seed culture was performed according to example 1, the primary seed solution was inoculated into 100ml of secondary seed medium for secondary seed culture, and after 48 hours of culture, 3g of triketone crushed material, 0.05g of tween-80, 0.5g of simethicone, 0.5g of PMS were added to the cultured secondary seeds, and the conversion conditions: 30 ℃, 200rpm, the conversion time is 64h, and after the conversion is finished, the HPLC is checked, the conversion rate of the trione dehydrogenation product is 22.78 percent, and the conversion rate of the trione is 72.60 percent.
Comparative example 5
Shake flask seed culture was performed according to example 1, the primary seed solution was inoculated into 100ml of secondary seed medium for secondary seed culture, and after 48 hours of culture, 3g of triketone crushed material, 0.05g of tween-80, 0.5g of PMS, 3ml of ethanol were added to the cultured secondary seeds, and the transformation conditions: 30 ℃, 200rpm, the conversion time is 64h, and after the conversion is finished, the HPLC is checked, the conversion rate of the trione dehydrogenation product is 24.78 percent, and the conversion rate of the trione is 69.50 percent.
Comparative example 6
Shake flask seed culture was performed according to example 1, the primary seed solution was inoculated into 100ml of secondary seed medium for secondary seed culture, and after 48 hours of culture, 3g of triketone crushed material, 0.05g of tween-80, 0.5g of PPE, 0.5g of pms, 3ml of ethanol were added to the cultured secondary seeds, and the transformation conditions: 30 ℃, 200rpm, the conversion time is 64h, and after the conversion is finished, the HPLC is checked, the conversion rate of the trione dehydrogenation product is 84.62 percent, and the conversion rate of the trione is 13.02 percent.
Comparative example 7
Shake flask seed culture was performed according to example 1, the primary seed solution was inoculated into 100ml of secondary seed medium for secondary seed culture, and after 48 hours of culture, 3g of triketone crushed material, 0.05g of tween-80, 0.5g of simethicone, 0.5g of pms, 3ml of ethanol were added to the cultured secondary seeds, and the transformation conditions: 30 ℃, 200rpm, the conversion time is 64h, and after the conversion is finished, the HPLC is checked, the conversion rate of the trione dehydrogenation product is 38.53 percent, and the conversion rate of the trione is 58.89 percent.
Comparative example 8
The shake flask seed culture was performed according to example 1, the primary seed solution was inoculated into 100ml of secondary seed medium for secondary seed culture, and the culture was carried outAfter 48h of cultivation, 3g of triketone crushed material, 0.05g of Tween-80 and 0.5g of PPG are added into the cultured second-level seeds3000(polypropylene glycol 3000), 0.5g pms, conversion conditions: 30 ℃, 200rpm, the conversion time is 64h, and after the conversion is finished, the HPLC is checked, the conversion rate of the trione dehydrogenation product is 54.05 percent, and the conversion rate of the trione is 40.15 percent.
Comparative example 9
Shaking seed culture according to example 1, inoculating the primary seed solution into 100ml of secondary seed culture medium for secondary seed culture, culturing for 48 hr, adding 3g triketone crushed material, 0.05g Tween-80, and 0.5g PPG into the cultured secondary seed30000.5g of PMS, 3ml of ethanol, transformation conditions: 30 ℃, 200rpm, the conversion time is 64h, and after the conversion is finished, the HPLC is checked, the conversion rate of the trione dehydrogenation product is 75.42 percent, and the conversion rate of the trione is 16.02 percent.
Comparative example 10
Shake flask seed culture was performed according to example 1, the primary seed solution was inoculated into 100ml of secondary seed medium for secondary seed culture, and after 48 hours of culture, 3g of triketone crushed material, 0.05g of tween-80, 0.5g of PPE, 0.5g of pms, 3ml of methanol, transformation conditions were added to the cultured secondary seeds: 30 ℃, 200rpm, the conversion time is 64h, and after the conversion is finished, the HPLC is checked, the conversion rate of the trione dehydrogenation product is 83.74 percent, and the conversion rate of the trione is 13.70 percent.
Comparative example 11
Shake flask seed culture was performed according to example 1, the primary seed solution was inoculated into 100ml of secondary seed medium for secondary seed culture, and after 48 hours of culture, 3g of triketone ground material, 0.05g of tween-80, 0.5g of PPE, 0.5g of PMS, 3ml of DMSO, transformation conditions were added to the cultured secondary seeds: 30 ℃, 200rpm, the conversion time is 64h, and after the conversion is finished, the HPLC is checked, the conversion rate of the trione dehydrogenation product is 29.45 percent, and the conversion rate of the trione is 68.04 percent.
Comparative example 12
Shake flask seed culture was performed according to example 1, the primary seed solution was inoculated into 100ml of secondary seed medium for secondary seed culture, and after 10-72h of culture, 3g of triketone ground material, 0.05g of tween-80, 0.5g of PPE, 0.5g of PMS, 3ml of DMF were added to the cultured secondary seeds, and the transformation conditions: 30 ℃, 50-250rpm, the conversion time is 64h, and after the conversion is finished, the HPLC is checked, the conversion rate of the trione dehydrogenation product is 78.76 percent, and the conversion rate of the trione is 16.39 percent.
Comparative example 13
Shake flask seed culture was performed according to example 1, the primary seed solution was inoculated into 100ml of secondary seed medium for secondary seed culture, and after 48 hours of culture, 3g of triketone crushed material, 0.05g of tween-80, 0.5g of PPE, 0.5g of PMS, 3ml of acetone were added to the cultured secondary seeds, and the transformation conditions: 30 ℃, 200rpm, the conversion time is 64h, and after the conversion is finished, the HPLC is checked, the conversion rate of the trione dehydrogenation product is 79.91 percent, and the conversion rate of the trione is 18.08 percent.
Example 2
Shake flask seed culture was performed according to example 1, the primary seed solution was inoculated into 100ml of secondary seed medium for secondary seed culture, and after 48 hours of culture, 3g of triketone crushed material, 0.05g of tween-80, 0.5g of PPE, 0.5g of pms, transformation conditions were added to the cultured secondary seeds: 30 ℃, 200rpm, the conversion time is 64h, and after the conversion is finished, the HPLC is checked, the conversion rate of the trione dehydrogenation product is 88.39 percent, and the conversion rate of the trione is 9.22 percent.
Example 3
Shake flask seed culture was performed according to example 1, the primary seed solution was inoculated into 100ml of secondary seed medium for secondary seed culture, and after 48 hours of culture, 3g of triketone ground material, 0.05g of tween-80, 0.5g of PMS, 1g of PPE, which is a polyether defoamer, were added to the cultured secondary seeds, and the conversion conditions were: 30 ℃, 200rpm, the conversion time is 64h, and after the conversion is finished, the HPLC is checked, the conversion rate of the trione dehydrogenation product is 89.70 percent, and the conversion rate of the trione is 7.965 percent.
Example 4
Shake flask seed culture was performed according to example 1, the primary seed solution was inoculated into 100ml of secondary seed medium for secondary seed culture, and after 48 hours of culture, 3g of triketone ground material, 0.05g of tween-80, 0.5g of PMS, 2g of PPE, which is a polyether defoamer, were added to the cultured secondary seeds, and the conversion conditions were: 30 ℃, 200rpm, conversion time 64h, and after the conversion is finished, sending HPLC to check that the conversion rate of the trione dehydrogenation product is 92.67 percent and the conversion rate of the trione is 5.62 percent (figure 1).
Example 5
Shake flask seed and fermenter seed culture were performed according to example 1, and after 24h of culture, 18g of triketone crushed material, 0.3g of tween-80, 12g of PPE, 3g of PMS, were added to the cultured fermenter seeds, and the conversion conditions were: 30 ℃, 500rpm, 0.2m30.05MPa, 64h of conversion time, and after the conversion is finished, sending and detecting HPLC, wherein the conversion rate of the trione dehydrogenation product is 93.04 percent, and the conversion rate of the trione is 5.17 percent (figure 2).
The analysis of the additive components and the analysis of the conversion of the product and the raw material of the specific examples and comparative examples are shown in Table 1.
TABLE 1 analysis of additive components and conversion of product and raw material in examples and comparative examples
From the HPLC data, different types of antifoam, besides having a greater effect on the conversion results, indicate that these antifoam play not only an antifoaming role, but also a solubilizing and dispersing role.
Those of ordinary skill in the art will understand that: the discussion of any embodiment above is meant to be exemplary only, and is not intended to imply that the scope of the application is limited to these examples; within the context of the present application, features from the above embodiments or from different embodiments may also be combined, steps may be implemented in any order, and there are many other variations of different aspects of one or more embodiments in the present application as described above, which are not provided in detail for the sake of brevity.
It is intended that the one or more embodiments of the present application cover all such alternatives, modifications, and variations as fall within the broad scope of the present application. Therefore, any omissions, modifications, substitutions, improvements, and the like that may be made without departing from the spirit and principles of one or more embodiments of the present application are intended to be included within the scope of the present application.
Claims (7)
1. A preparation method of a trione dehydrogenated substance is characterized in that a microorganism containing 3-sterone-1-dehydrogenase is mixed with raw material trione, a surfactant, polyether defoamer PPE and an exogenous electron acceptor are added at the same time, no organic solvent is added, microorganism fermentation is carried out, and the trione dehydrogenated substance is obtained after the fermentation is finished, wherein the reaction route is as follows:
2. the method for producing a triketone dehydrogenase according to claim 1, wherein the microorganism containing 3-sterone-1-dehydrogenase is Arthrobacter simplex.
3. The method according to claim 1, wherein the surfactant is tween-80.
4. The process for producing a triketone dehydrogenase as claimed in claim 1, wherein the exogenous electron acceptor is an exogenous electron acceptor PMS.
5. The process for preparing the triketone dehydrogenator as claimed in any of claims 1 to 4, wherein the weight ratio of starting triketone to the polyether antifoam PPE is from 3:0.5 to 2.
6. The process according to any one of claims 1 to 4, wherein the starting material trione is present in the fermentation system in a concentration of 0.1 to 10% by weight, the surfactant is present in a concentration of 0.01 to 0.5% by weight, the polyether defoamer PPE is present in a concentration of 0.1 to 5% by weight, and the exogenous electron acceptor is present in a concentration of 0.001 to 0.5% by weight.
7. The trione compound of any one of claims 1 to 4The preparation method of the hydrogen is characterized in that the fermentation temperature is 25-32 ℃, the rotating speed is 200-800rpm, and the ventilation volume is 0.2-0.4m3The tank pressure is 0.02-0.1 MPa.
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| CN104263791A (en) * | 2014-09-12 | 2015-01-07 | 苏州汉酶生物技术有限公司 | Method for preparing 11A, 17A-dihydroxy-pregna-1,4-diene-3,20-dione by enzymatic method |
| CN110714047A (en) * | 2019-11-19 | 2020-01-21 | 湖南新合新生物医药有限公司 | Method for dehydrogenation of steroid intermediate 1, 2-position |
| CN112608970A (en) * | 2020-12-25 | 2021-04-06 | 河南利华制药有限公司 | Production method of methylprednisolone dehydrogenation product |
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| CN110714047A (en) * | 2019-11-19 | 2020-01-21 | 湖南新合新生物医药有限公司 | Method for dehydrogenation of steroid intermediate 1, 2-position |
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