CN103484375A - Fusarium oxysporum SC1301 capable of biologically synthesizing testolactone, and synthesis method - Google Patents
Fusarium oxysporum SC1301 capable of biologically synthesizing testolactone, and synthesis method Download PDFInfo
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Abstract
The present invention discloses a strain of Fusarium oxysporum SC1301 and a method for producing testolactone through fermentation conversion of an androstane or pregnane steroid compound by using the Fusarium oxysporum SC1301. According to the present invention, the Fusarium oxysporum SC1301 adopts androsta-1,4-diene-3,17-dione as a sole carbon source, is screened from soil collected from the Xiqing district farm orchard in Tianjin city, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), wherein the preservation number is CGMCC No.5962; and the production method comprises: adopting Fusarium oxysporum SC1301 as bacteria, carrying out primary seed culture and secondary seed culture, pouring a crushed androstane or pregnane steroid compound or an androstane or pregnane steroid compound dissolved through a solvent into a bacterial fermentation liquid to carry out a conversion reaction, and carrying out organic solvent extraction and silica gel column chromatography separation to obtain the product testolactone, wherein yields are greater than 78%, and operations are simple and energy consumption is low during the reaction process.
Description
Technical field:
The present invention relates to bioengineering field, the present invention relates to a kind of bacterial strain and synthetic method of biosynthesizing testolactone.
Background technology:
Testolactone is the derivative of testosterone, English name Testolactone or 1,2-dehydrotestololactone etc., chemical formula C
19h
24o
6, formula weight 300.39, white or off-white color crystalline powder, 218～219 ℃ of fusing points.D23+45.6 ° of specific rotation [α] (chloroform), CAS968-93-4.Testolactone is a kind of aromatase inhibitor, can stop testosterone to be converted into estradiol, Androstenedione is converted into oestrone, testolactone and derivative thereof are used as the diseases (Dunkel L.Use of aromatase inhibitors to increase final height.Molecular and Cellular Endocrinology.2006,254:207) such as cell toxicant anticarcinogen and treatment male sterility clinically.
The open loop oxygenation process that is generated testolactone by etioallocholane or pregnane steroidal compounds is mainly concerned with Baeyer-Villiger reaction (BVMO).This reaction is 1899, Baeyer and Villiger report under the effect of oxygenant (being generally peracid) by acyclic ketogenesis ester, cyclic ketone generates method (the Leisch H of lactone, Morler K, Lau P C K.Baeyer-Villiger Monooxygenases:More Than Just Green Chemistry.Chemical Reviews, 2011,111 (7): 4165-4222).Main employing peroxide agent on organic chemical synthesis, but the conventional oxidation agent is poisonous and/or explosive mostly, and nearly all product is all raceme.For obtaining suitable enantioselectivity, some oxo transition metal agents and noble metal part occur, and needed low-temp reaction, this method reagent is more expensive, and severe reaction conditions, therefore only has theoretical significance.
1948, Turfitt is when the bio-transformation of a series of steroidals of research, find first microorganism energy catalysis BVMO reaction (Turfitt, G E.The microbiological degradation of steroids:4.Fission of the steroid molecule.The Biochemical Journal, 1948,42 (3): 376-383).Follow-up bibliographical information shows, the BVMO reaction can be by a large amount of bacteriums (as genus arthrobacter, Nore card Pseudomonas, Rhod, chain enzyme bacteria genus etc.) and fungi (curvularia lunata, Eurotium, Fusarium, Penicillium etc.) catalysis (Kolek T, Szpineter A, S ' wizdor A.Baeyer-Villiger oxidation of DHEA, pregnenolone, and Androsten-edione by Penicillium lilacinum AM111.Steroids, 2008,73:1441-1445).
Up to the present, the microorganism that energy catalysis etioallocholane or pregnane steroidal compounds generate testolactone is mainly mould and aspergillus, Penicillium citreo-viride (Liu H M for example, Li H P, Shan L H.Synthesis of steroidal lactone by penicillium citreo-viride.Steroids, 2006, 71 (11-12): 931-934), terreus (Kudret Y, Ahmet U, Yasemin G E.Biotransformation of some steroids by Aspergillus terreus MRC 200365.Collection of Czechoslovak Chemical Communications.2010, 75 (6): 665-673), Aspergillus tamarii (Kudret Y, Ahmet U, Yasemin G E.Baeyer-Villiger oxidation of some steroids by Aspergillus tamarii MRC 72400.Collection of Czechoslovak Chemical Communications.2011, 76 (6): 743-754), mould (the Agnieszka B of soil, Jadwiga D G. Transformation of steroids by Trichoderma hamatum.Enzyme and Microbial Technology.2007, 40 (6): 1615-1621) etc., but there are the problems such as the long or product of transformation time is not single.
In process of the present invention, by a large amount of microbe to screen, found first a kind of Fusarium oxysporum Fusarium oxysporum SC1301, several steroid materials of energy Efficient Conversion, obtain the product testolactone.Product is single and speed is fast, and does not need to add coenzyme system in conversion process, has solved coenzyme in conversion process and has added and the electronics problem of transmission.The Fusarium oxysporum Fusarium oxysporum SC1301 fast growth that screening obtains and easily cultivation, catalysis is stable, and environmental compatibility is strong.The catalytic reaction condition gentleness, reaction process is simple, easy handling, productive rate is high, and by product is few, and energy consumption is low.
Summary of the invention
The purpose of this invention is to provide a kind of can the Efficient Conversion etioallocholane or the pregnane steroidal compounds generate the Fusarium oxysporum of testolactone.
Another object of the present invention is to provide a kind of method of new synthetic testolactone, it is characterized in that, take etioallocholane or pregnane steroidal compounds is that formula (1) compound is substrate, utilizes the Fusarium oxysporum bio-transformation to obtain formula (2) testolactone.
Reaction formula is as follows:
R
1for carbonyl or beta-hydroxy;
R
2for carbonyl or β-ethanoyl;
1,2,4,5,6,7 is singly-bound or two key.
Sharp sickle spore (Fusarium oxysporum) SC1301 of a strain biosynthesizing testolactone provided by the invention separates, screens the fungal strain obtained the soil gathered from Tianjin Xiqing District farmers' orchard.This bacterial strain on March 29th, 2012 in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, Classification And Nomenclature is sharp sickle spore (Fusarium oxysporum), deposit number is CGMCC No.5962.
The morphological specificity of described Fusarium oxysporum SC1301 is as follows: this bacterial strain is rapid in the dull and stereotyped growth of PDA, the bacterium colony circle, and white hypha, crawl to spread growth, and mycelia is flocculence, grows intensive.Bacterium colony has an even surface, and bacterium colony back side color is faint yellow, and bacterium colony is combined tightr with substratum.Micro-Microscopic observation mycelium is transparent, branch without every, the mycelium top produces white conidium, spore is sickleshaped, and barrier film is arranged.
The rDNA-ITS gene sequence characteristic of this bacterial strain: its rDNA-ITS has the nucleotide sequence as shown in sequence table, and sequence length is 480bp.
According to its morphological specificity and rDNA-ITS gene order, identify that this bacterial strain is Fusarium oxysporum (Fusarium oxysporum).
Use the method that Fusarium oxysporum conversion etioallocholane of the present invention or pregnane steroidal compounds are testolactone, comprise the steps:
(1) seed and fermentation culture: get Fusarium oxysporum Fusarium oxysporum SC1301 bacterial classification and be inoculated in the first order seed substratum, 30～35 ℃, 200r/min cultivates 48h-72h, obtains seed culture fluid.With inoculum size 5%～10%, transfer in fresh fermention medium, 30～35 ℃, 200r/min carries out the second order fermentation cultivation, cultivates 24～30h and obtains the thalline fermented liquid.
(2) conversion reaction: be that compound (1) is pulverized or, with in the complete molten or half molten input thalline fermented liquid of solvent, 30～35 ℃, 150～200r/min transforms 9～24h termination reaction by steroidal compounds.Described solvent is selected from but is not limited only to a kind of of acetone, ethyl acetate, methyl alcohol, ethanol, DMSO (dimethyl sulfoxide (DMSO)), when above-mentioned pulverizing feeds intake, in fermented liquid, adds chaotropic agent, and described chaotropic agent is tween 80.
(3) extraction of product is with refining: after step (2) conversion reaction finishes, be extracted with ethyl acetate fermented liquid for several times, and combining extraction liquid, drying, concentrating under reduced pressure, adopt silica gel chromatographic column to separate after concentrating and obtain testolactone.
Described bio-transformation substratum used adopts following proportioning:
Seed culture medium: glucose 10～15g/L, glycerine 12.63g/L, peptone 5g/L, yeast extract 5g/L, NaCl2g/L, KH
2pO
45g/L, the pH nature.
Fermention medium: glucose 30g/L, Dried Corn Steep Liquor Powder 10～15g/L, NaNO
32g/L, KCl 0.5g/L, K
2hPO
43H
2o 2g/L, KH
2pO
41g/L, MgSO
47H
2o 0.5g/L, FeSO
47H
2o 0.02g/L, pH 8.0.
Described steroidal compounds is any one in the preferred following compound of compound (1):
(1) 4-AD
(2) DELTA4-pregn-3,20-dione
(3) 3 beta-hydroxies-5-androstene-17-ketone
(4) Androsta-1,4-diene-3,17-dione
The concentration that feeds intake as the compound (1) of substrate is 0.1～2g/L.
Beneficial effect of the present invention: sample from nature, found a kind of bacterial strain Fusarium oxysporum SC1301 that can transform etioallocholane or pregnane steroidal compounds generation testolactone, product is single and speed is fast, and do not need to add coenzyme system in conversion process, solved coenzyme in conversion process and added and the electronics problem of transmission.Theoretical yield is 100%, reclaims productive rate 78～93%.The Fusarium oxysporum SC1301 fast growth that screening obtains and easily cultivation, after inoculation, 12h enters logarithmic phase, and 30h arrives stationary phase.Catalysis is stable, and environmental compatibility is strong.The catalytic reaction condition gentleness, reaction process is simple, easy handling, productive rate is high, and by product is few, and energy consumption is low.
The accompanying drawing explanation
Shown in Fig. 1 is the colonial morphology figure of Fusarium oxysporum Fusarium oxysporum SC1301;
Shown in Fig. 2 is the spore shape figure of Fusarium oxysporum Fusarium oxysporum SC1301;
Be based on the rDNA-ITS sequence shown in Fig. 3, the figure of Fusarium oxysporum Fusarium oxysporum SC1301 and relevant bacterial strain systematic evolution tree;
Shown in Fig. 4 is the testolactone that embodiment makes
1h-NMR nucleus magnetic resonance figure;
Shown in Fig. 5 is the crystalline structure X-ray diffraction figure of the testolactone that makes of embodiment.
Embodiment
The following examples further illustrate content of the present invention, but should not be construed as limitation of the present invention.
Embodiment 1: the screening of bacterial strain and evaluation
1.1 substratum
Minimal medium: K
2hPO
43H
2o 1.0g, Na
2hPO
43H
2o 1.0g, (NH
4)
2hPO
42.0g, NaNO
32.0g, MgSO
47H
2o 0.2g, CaCl
22H
2o 10mg, FeSO
47H
2o 1.0mg, ZnSO
40.1mg, distilled water 1000mL.
In minimal medium, add certain density Androsta-1,4-diene-3,17-dione to be made into corresponding enrichment medium.
1.2 the separation and purification of bacterial strain
The sample of collection is got to 5g and put into the 250mL shaking flask that contains the 50mL sterilized water, add granulated glass sphere, 2h vibrates on the shaking table of 250r/min, standing 3-5min, after the soil particle precipitation, therefrom getting the 2mL supernatant joins in 50mL liquid enrichment medium (containing Androsta-1,4-diene-3,17-dione 1g/L).30 ℃, cultivate 2-3 days on the 200r/min shaking table, get the dilution of enrichment culture liquid and be coated with dull and stereotyped (containing the solid enrichment medium of Androsta-1,4-diene-3,17-dione 1g/L), separate, purifying is until screening obtains single bacterium colony, by pure colony inoculation to inclined-plane.
1.3 bacterial strain is converted into Androsta-1,4-diene-3,17-dione the mensuration of testolactone ability
In the single bacterial strain after purifying is from the inclined plane inoculating to the liquid fermentation medium, with the nutrient solution that does not connect bacterium, compare, 30 ℃, when on the 200r/min shaking table, shaking culture is to the obvious muddiness of substratum, add 1g/L androstane-Isosorbide-5-Nitrae-diene-3, the 17-diketone starts reaction, transform respectively 12h, 24h, sampling analysis after 48h.Get the fermented liquid of certain volume, by isopyknic ethyl acetate extracting twice, each vortex vibration 10min is to extract fully, and the centrifugal 10min of 12000r/min, get supernatant combining extraction liquid, carries out the TLC analysis.
Screen and obtain the bacterial strain that a strain energy Efficient Conversion Androsta-1,4-diene-3,17-dione is testolactone by aforesaid method.This bacterial strain bacterium colony circle, white hypha, crawl to spread growth, and mycelia is flocculence, grows intensive.Bacterium colony has an even surface, and bacterium colony back side color is faint yellow, and bacterium colony is combined tightr with substratum, as shown in Figure 1.Micro-Microscopic observation mycelium is transparent, branch without every, the mycelium top produces white conidium, spore is sickleshaped, and barrier film is arranged, as shown in Figure 2.The total DNA of this bacterial strain of further take is template, with the universal primer of rDNA-ITS gene, carries out pcr amplification, obtains amplified fragments and is reclaimed, checks order.Through order-checking, its sequence is as shown in sequence table, and the known array in this sequence and GenBank database carries out the BLAST compare of analysis, and obtains relevant kind rDNA-ITS sequence from database, phylogenetic tree construction, as shown in Figure 3.According to morphological specificity and rDNA-ITS gene order thereof, identify that this bacterial strain is Fusarium oxysporum.
Embodiment 2: the method that the 4-AD of take synthesizes testolactone as substrate
1 preparation seed liquor
Get Fusarium oxysporum Fusarium oxysporum SC1301 bacterial classification and be inoculated in the first order seed substratum, in 30 ℃ of cultivation 48h, shaking speed 200r/min, obtain seed culture fluid.
Described seed culture medium is pressed following proportional arrangement: glucose 10g/L, glycerine 12.63g/L, peptone 5g/L, yeast extract 5g/L, NaCl 2g/L, KH
2pO
45g/L, the pH nature.
2 second order fermentations are cultivated
The seed culture fluid that step 1 is obtained is transferred in fresh fermention medium by inoculum size 5%, culture condition: 30 ℃, and shaking speed 200r/min, fermentation time 30h, obtain the thalline fermented liquid.
The composition of described fermention medium is: glucose 30g/L, Dried Corn Steep Liquor Powder 10g/L, NaNO
32g/L, KCl 0.5g/L, K
2hPO
43H
2o 2g/L, KH
2pO
41g/L, MgSO
47H
2o 0.5g/L, FeSO
47H
2o 0.02g/L, pH 8.0.
3 conversion reactions
Add the 4-AD that dissolves preparation with DMSO in the thalline fermented liquid, concentration of substrate is 1g/L, 30 ℃, and termination reaction after 200r/min conversion 15h.
The extraction and identification of 4 products
After reaction finishes, be extracted with ethyl acetate fermented liquid for several times, combining extraction liquid, anhydrous Na
2sO
4drying, concentrating under reduced pressure, the concentrated rear silica gel chromatographic column separated product that adopts, elutriant is ethyl acetate: sherwood oil=7: 3, productive rate is 82.9%.
Embodiment 3: the method that the DELTA4-pregn-3,20-dione of take synthesizes testolactone as substrate
With embodiment 1, Fusarium oxysporum Fusarium oxysporum SC1301 is carried out to one-level cultivation and secondary cultivation, add the pregnant Gona-4-ene-3 that dissolves preparation with DMSO in the thalline fermented liquid, the 20-diketone, concentration of substrate is 1g/L, 30 ℃, and termination reaction after 200r/min conversion 15h.After reaction finishes, be extracted with ethyl acetate fermented liquid for several times, combining extraction liquid, anhydrous Na
2sO
4drying, concentrating under reduced pressure, the concentrated rear silica gel chromatographic column separated product that adopts, elutriant is ethyl acetate: sherwood oil=7: 3, productive rate is 93%.
Embodiment 4: take 3 beta-hydroxies-5-androstene-17-ketone as the synthetic testolactone method of substrate
With embodiment 1, Fusarium oxysporum Fusarium oxysporum SC1301 is carried out to one-level is cultivated and secondary is cultivated, add in the thalline fermented liquid with DMSO and dissolve 3 beta-hydroxies prepared-5-androstene-17-ketone, concentration of substrate is 1g/L, 30 ℃, termination reaction after 200r/min conversion 24h.After reaction finishes, be extracted with ethyl acetate fermented liquid for several times, combining extraction liquid, anhydrous Na
2sO
4drying, concentrating under reduced pressure, the concentrated rear silica gel chromatographic column separated product that adopts, elutriant is ethyl acetate: sherwood oil=5: 5, productive rate is 78%.
Embodiment 5: the method that the Androsta-1,4-diene-3,17-dione of take synthesizes testolactone as substrate
With embodiment 1, Fusarium oxysporum Fusarium oxysporum SC1301 is carried out to one-level cultivation and secondary cultivation, add the androstane-1 that dissolves preparation with DMSO in the thalline fermented liquid, 4-diene-3, the 17-diketone, concentration of substrate is 1g/L, 30 ℃, termination reaction after 200r/min conversion 15h.After reaction finishes, be extracted with ethyl acetate fermented liquid for several times, combining extraction liquid, anhydrous Na
2sO
4drying, concentrating under reduced pressure, the concentrated rear silica gel chromatographic column separated product that adopts, elutriant is ethyl acetate: sherwood oil=7: 3, productive rate is 82.3%.
In above example, products therefrom testolactone structure detection data are as follows:
1h NMR: δ (ppm): 7.02 (d, J=10.2Hz, 1H), (6.26 dd, J=10.2Hz, 1H), (6.09 s, 1H), 2.72-2.55 (m, 2H), 2.50-2.40 (m, 2H), (2.16-1.96 m, 4H), 1.70-1.65 (m, 2H), 1.62-1.50 (m, 2H), (1.48-1.42 m, 1H), 1.39 (s, 3H), 1.30-1.26 (m, 1H), 1.22 (s, 3H), 1.16-1.09 (m, 1H). (seeing accompanying drawing 4); Crystalline structure X-ray diffraction (seeing accompanying drawing 5).
Claims (6)
1. Fusarium oxysporum Fusarium oxysporum SC1301, depositary institution is Chinese common micro-organisms DSMZ, deposit number is CGMCC No.5962.
2. the fermentation culture method of the described bacterial strain of claim 1, it comprises step:
1) seed culture
By in the seed liquid nutrient medium after Fusarium oxysporum Fusarium oxysporum SC1301 access sterilizing, in 30～35 ℃, 200r/min cultivates 48～72h, obtains seed liquor,
Being formulated as follows of liquid nutrient medium: glucose 10～15g/L, glycerine 12.63g/L, peptone 5g/L, yeast extract 5g/L, NaCl 2g/L, KH
2pO
45g/L, the pH nature;
2) fermentation culture
The seed liquid that step (1) is obtained is inoculated in fermention medium and carries out fermentation culture by inoculum size 5～10%, culture condition: 30～35 ℃, and shaking speed 200r/min, fermentation time 24～30h, obtain the fermented liquid of thalline,
The composition of described fermention medium is: glucose 30g/L, Dried Corn Steep Liquor Powder 10～15g/L, NaNO
32g/L, KCl 0.5g/L, K
2hPO
43H
2o 2g/L, KH
2pO
41g/L, MgSO
47H
2o 0.5g/L, FeSO
47H
2o 0.02g/L, pH 8.0.
3. a biosynthetic means for preparing testolactone, is characterized in that, take etioallocholane or pregnane steroidal compounds is that formula (1) compound is substrate, adopts the described bacterial strain bio-transformation of claim 1 to obtain formula (2) testolactone,
Reaction formula is as follows:
R
1for carbonyl or beta-hydroxy;
R
2for carbonyl or β-ethanoyl;
1,2,4,5,6,7 is singly-bound or two key.
Comprise the steps:
1) conversion reaction: fermentation culture bacterial strain claimed in claim 1, formula (1) compound is pulverized or, with dissolution with solvents, dropped in cultured thalline fermented liquid and carry out bio-transformation, 30～35 ℃, 150～200r/min transforms 9～24h termination reaction;
2) extraction of product is with refining: after step (1) conversion reaction finishes, be extracted with ethyl acetate fermented liquid for several times, and combining extraction liquid, drying, concentrating under reduced pressure, adopt silica gel chromatographic column to separate after concentrating and obtain testolactone.
4. biosynthetic means as claimed in claim 3, it is characterized in that: the solvent of described dissolving substrate is selected from but is not limited only to a kind of of acetone, ethyl acetate, methyl alcohol, ethanol, dimethyl sulfoxide (DMSO), preferred dimethyl sulfoxide (DMSO), add-on is 1～4% (v/v).
5. biosynthetic means as claimed in claim 3 is characterized in that a kind of of the preferably following several compounds of the described compound as substrate (1):
4-AD;
DELTA4-pregn-3,20-dione;
3 beta-hydroxies-5-androstene-17-ketone;
Androsta-1,4-diene-3,17-dione.
6. those skilled in the art can make according to the present invention various changes or distortion, only otherwise break away from technological thought of the present invention, all belong to the defined scope of the claims in the present invention.
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| CN105670947A (en) * | 2016-04-27 | 2016-06-15 | 山东省农业科学院植物保护研究所 | Setaria viridis-containing fusarium culture medium and preparation method thereof |
| CN106591155A (en) * | 2017-01-13 | 2017-04-26 | 郑州大学 | Fusarium oxysporum strain and application thereof in preparation of 3beta, 7alpha, 15alpha-trihydroxy androstane-5-ene-17-ketone |
| CN108707553A (en) * | 2018-05-10 | 2018-10-26 | 上海师范大学 | It is capable of bacterial strain and its application of Efficient Conversion 4AD specificity synthesis keto lactone clonorchis and ADD |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105670947A (en) * | 2016-04-27 | 2016-06-15 | 山东省农业科学院植物保护研究所 | Setaria viridis-containing fusarium culture medium and preparation method thereof |
| CN105670947B (en) * | 2016-04-27 | 2019-11-26 | 山东省农业科学院植物保护研究所 | A kind of reaping hook bacterium culture medium and preparation method thereof containing herba setariae viridis |
| CN106591155A (en) * | 2017-01-13 | 2017-04-26 | 郑州大学 | Fusarium oxysporum strain and application thereof in preparation of 3beta, 7alpha, 15alpha-trihydroxy androstane-5-ene-17-ketone |
| CN106591155B (en) * | 2017-01-13 | 2019-07-23 | 郑州大学 | Fusarium oxysporum strain and its 3 β, 7 α are being prepared, the application in 15 α-trihydroxyandrost -5- alkene -17- ketone |
| CN108707553A (en) * | 2018-05-10 | 2018-10-26 | 上海师范大学 | It is capable of bacterial strain and its application of Efficient Conversion 4AD specificity synthesis keto lactone clonorchis and ADD |
| CN108707553B (en) * | 2018-05-10 | 2020-10-16 | 上海师范大学 | Strain capable of efficiently converting 4AD specificity to synthesize testosterone and ADD and application thereof |
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