CN114317652A - 一种利用多孔聚合物分离材料从鱼籽多肽中分离纯化具有改善记忆功能的多肽的方法 - Google Patents
一种利用多孔聚合物分离材料从鱼籽多肽中分离纯化具有改善记忆功能的多肽的方法 Download PDFInfo
- Publication number
- CN114317652A CN114317652A CN202111541323.8A CN202111541323A CN114317652A CN 114317652 A CN114317652 A CN 114317652A CN 202111541323 A CN202111541323 A CN 202111541323A CN 114317652 A CN114317652 A CN 114317652A
- Authority
- CN
- China
- Prior art keywords
- polypeptide
- roe
- fish
- porous polymer
- fish roe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 67
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 63
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 61
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 28
- 230000006870 function Effects 0.000 title claims abstract description 21
- 238000000926 separation method Methods 0.000 title claims abstract description 20
- 239000000463 material Substances 0.000 title claims abstract description 10
- 229920000642 polymer Polymers 0.000 title claims abstract description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 35
- 239000002861 polymer material Substances 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 102000004142 Trypsin Human genes 0.000 claims abstract description 9
- 108090000631 Trypsin Proteins 0.000 claims abstract description 9
- 239000012588 trypsin Substances 0.000 claims abstract description 9
- 238000011068 loading method Methods 0.000 claims abstract description 8
- 102000029816 Collagenase Human genes 0.000 claims abstract description 7
- 108060005980 Collagenase Proteins 0.000 claims abstract description 7
- 229960002424 collagenase Drugs 0.000 claims abstract description 7
- 239000008367 deionised water Substances 0.000 claims abstract description 7
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 7
- 239000006228 supernatant Substances 0.000 claims abstract description 7
- 238000005406 washing Methods 0.000 claims abstract description 4
- 238000001035 drying Methods 0.000 claims abstract description 3
- 238000004108 freeze drying Methods 0.000 claims abstract description 3
- 239000002245 particle Substances 0.000 claims description 6
- 239000011148 porous material Substances 0.000 claims description 6
- 239000002904 solvent Substances 0.000 claims description 2
- 239000000047 product Substances 0.000 claims 1
- 239000002994 raw material Substances 0.000 claims 1
- 238000002360 preparation method Methods 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 238000010828 elution Methods 0.000 abstract description 2
- 238000011049 filling Methods 0.000 abstract description 2
- 235000019688 fish Nutrition 0.000 description 25
- 238000009777 vacuum freeze-drying Methods 0.000 description 9
- 238000000746 purification Methods 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 241000700159 Rattus Species 0.000 description 6
- 229940088598 enzyme Drugs 0.000 description 6
- 238000010008 shearing Methods 0.000 description 6
- 229930000680 A04AD01 - Scopolamine Natural products 0.000 description 5
- STECJAGHUSJQJN-GAUPFVANSA-N Hyoscine Natural products C1([C@H](CO)C(=O)OC2C[C@@H]3N([C@H](C2)[C@@H]2[C@H]3O2)C)=CC=CC=C1 STECJAGHUSJQJN-GAUPFVANSA-N 0.000 description 5
- STECJAGHUSJQJN-UHFFFAOYSA-N N-Methyl-scopolamin Natural products C1C(C2C3O2)N(C)C3CC1OC(=O)C(CO)C1=CC=CC=C1 STECJAGHUSJQJN-UHFFFAOYSA-N 0.000 description 5
- STECJAGHUSJQJN-FWXGHANASA-N scopolamine Chemical compound C1([C@@H](CO)C(=O)O[C@H]2C[C@@H]3N([C@H](C2)[C@@H]2[C@H]3O2)C)=CC=CC=C1 STECJAGHUSJQJN-FWXGHANASA-N 0.000 description 5
- 229960002646 scopolamine Drugs 0.000 description 5
- 241001505070 Sinilabeo Species 0.000 description 3
- 230000000975 bioactive effect Effects 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 230000000415 inactivating effect Effects 0.000 description 3
- 238000005374 membrane filtration Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241001622901 Scomberomorus commerson Species 0.000 description 2
- 230000003064 anti-oxidating effect Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000009191 jumping Effects 0.000 description 2
- 229920000620 organic polymer Polymers 0.000 description 2
- 238000004007 reversed phase HPLC Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 108090000145 Bacillolysin Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241001596950 Larimichthys crocea Species 0.000 description 1
- 241001417045 Lophius litulon Species 0.000 description 1
- 102000035092 Neutral proteases Human genes 0.000 description 1
- 108091005507 Neutral proteases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241000269821 Scombridae Species 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 230000009189 diving Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 235000020640 mackerel Nutrition 0.000 description 1
- 230000006386 memory function Effects 0.000 description 1
- 230000006993 memory improvement Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000058 polyacrylate Polymers 0.000 description 1
- 239000004584 polyacrylic acid Substances 0.000 description 1
- 229920000193 polymethacrylate Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 229920003053 polystyrene-divinylbenzene Polymers 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
Images
Landscapes
- Peptides Or Proteins (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111541323.8A CN114317652A (zh) | 2021-12-16 | 2021-12-16 | 一种利用多孔聚合物分离材料从鱼籽多肽中分离纯化具有改善记忆功能的多肽的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111541323.8A CN114317652A (zh) | 2021-12-16 | 2021-12-16 | 一种利用多孔聚合物分离材料从鱼籽多肽中分离纯化具有改善记忆功能的多肽的方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN114317652A true CN114317652A (zh) | 2022-04-12 |
Family
ID=81052497
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202111541323.8A Pending CN114317652A (zh) | 2021-12-16 | 2021-12-16 | 一种利用多孔聚合物分离材料从鱼籽多肽中分离纯化具有改善记忆功能的多肽的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN114317652A (zh) |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998042213A1 (fr) * | 1997-03-24 | 1998-10-01 | Nippon Carbide Kogyo Kabushiki Kaisha | Produit semblable a des oeufs de poisson |
DE102007044093A1 (de) * | 2007-09-14 | 2009-03-19 | Phenion Gmbh & Co. Kg | Nukleinsäurehaltige kosmetische und/oder pharmazeutische Zubereitungen zur Induktion antimikrobieller Peptide in epithelialen Deckgeweben |
CN102286582A (zh) * | 2011-06-21 | 2011-12-21 | 华南理工大学 | 以深海鱼为原料制备具有改善记忆的生物活性肽的方法 |
CN105255978A (zh) * | 2015-10-13 | 2016-01-20 | 华南理工大学 | 具有改善记忆功效的凤尾鱼美拉德肽及其制备方法和用途 |
CN107226836A (zh) * | 2017-06-23 | 2017-10-03 | 广东华肽生物科技有限公司 | 一种具有改善记忆功效的多肽及其分离制备方法和应用 |
CN111455007A (zh) * | 2020-03-30 | 2020-07-28 | 中国科学院兰州化学物理研究所 | 一种从鱼籽中制备的改善记忆功能的多肽 |
CN111484893A (zh) * | 2020-04-24 | 2020-08-04 | 中国科学院兰州化学物理研究所 | 一种同步制备鱼籽精油和鱼籽功能肽的方法 |
CN112921063A (zh) * | 2021-04-12 | 2021-06-08 | 中国科学院兰州化学物理研究所 | 一种抗阿尔兹海默症的鱼籽源功能肽的制备方法、功能肽及应用 |
-
2021
- 2021-12-16 CN CN202111541323.8A patent/CN114317652A/zh active Pending
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998042213A1 (fr) * | 1997-03-24 | 1998-10-01 | Nippon Carbide Kogyo Kabushiki Kaisha | Produit semblable a des oeufs de poisson |
DE102007044093A1 (de) * | 2007-09-14 | 2009-03-19 | Phenion Gmbh & Co. Kg | Nukleinsäurehaltige kosmetische und/oder pharmazeutische Zubereitungen zur Induktion antimikrobieller Peptide in epithelialen Deckgeweben |
CN102286582A (zh) * | 2011-06-21 | 2011-12-21 | 华南理工大学 | 以深海鱼为原料制备具有改善记忆的生物活性肽的方法 |
CN105255978A (zh) * | 2015-10-13 | 2016-01-20 | 华南理工大学 | 具有改善记忆功效的凤尾鱼美拉德肽及其制备方法和用途 |
CN107226836A (zh) * | 2017-06-23 | 2017-10-03 | 广东华肽生物科技有限公司 | 一种具有改善记忆功效的多肽及其分离制备方法和应用 |
CN111455007A (zh) * | 2020-03-30 | 2020-07-28 | 中国科学院兰州化学物理研究所 | 一种从鱼籽中制备的改善记忆功能的多肽 |
CN111484893A (zh) * | 2020-04-24 | 2020-08-04 | 中国科学院兰州化学物理研究所 | 一种同步制备鱼籽精油和鱼籽功能肽的方法 |
CN112921063A (zh) * | 2021-04-12 | 2021-06-08 | 中国科学院兰州化学物理研究所 | 一种抗阿尔兹海默症的鱼籽源功能肽的制备方法、功能肽及应用 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101402671B (zh) | 从畜禽血液中同时分离纤维蛋白原和免疫球蛋白的方法 | |
KR100904631B1 (ko) | 트랜스 글루타민나제를 이용한 기능성 굴 효소 가수분해물및 그의 제조방법 | |
Porath | Fractionation of polypeptides and proteins on dextran gels | |
Abreu et al. | Bromelain separation and purification processes from pineapple extract | |
CN107574209B (zh) | 一种高精氨酸混合肽的制备方法及其在宫颈癌治疗中的应用 | |
CN112646003B (zh) | 一种方格星虫ace抑制肽及其制备方法和应用 | |
CN107298710A (zh) | 一种螺旋藻藻蓝蛋白的提取方法 | |
CN101584853A (zh) | 一种地龙蛋白多肽制剂 | |
CN114317652A (zh) | 一种利用多孔聚合物分离材料从鱼籽多肽中分离纯化具有改善记忆功能的多肽的方法 | |
CN101845089B (zh) | 一种适合规模化生产眼镜蛇蛇毒神经毒素并降低神经毒性的方法 | |
EP0110688B1 (en) | Angiogenic factor production | |
CN105085651A (zh) | 一种酪蛋白磷酸肽单体及其制备方法 | |
CN105648010B (zh) | 一种激活Nrf2-ARE通路的双髻鲨鱼肉抗氧化肽的制备方法 | |
CN102199192A (zh) | 一种从龟甲胶中分离功能多肽的方法 | |
CN101456898B (zh) | 一种使用氢键吸附色谱分离纯化多肽的方法 | |
Niederwieser | Use of neutral polystyrene resin for rapid desalting and fractionation of non-polar amino acids and non-polar oligopeptides | |
Sharifi et al. | Use of a urea and guanidine-HCl—propanol solvents system to purify a growth inhibitory glycopeptide by high-performance liquid chromatography | |
CN108570101A (zh) | 一种仿刺参体壁中抗菌肽的分离纯化方法 | |
JP2022529565A (ja) | コラゲナーゼタイプi、コラゲナーゼタイプii、中性プロテアーゼ、およびクロストリパインからなる群から選択される少なくとも1種の酵素のクロマトグラフィー精製 | |
CN113214160A (zh) | 一种无氨氮排放高效纯化组氨酸原料药的方法 | |
CN113045632B (zh) | 一种微囊藻毒素mc-lr的批量纯化方法 | |
RU2225441C1 (ru) | Способ получения препарата коллагеназы | |
CN1233654C (zh) | 羟基磷灰石复性及同时纯化基因重组蛋白质的方法 | |
Khodwe et al. | Purification of arginine by ion-exchange chromatographic from cottonseed de-oiled cake | |
CN1563086A (zh) | 法氏囊活性肽的制备工艺 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20220523 Address after: 730000 No. 18 Tianshui Middle Road, Chengguan District, Gansu, Lanzhou Applicant after: Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences Applicant after: Yantai Zhongke advanced materials and green chemical industry technology research institute Address before: 730030 No.18, Tianshui Middle Road, Chengguan District, Lanzhou City, Gansu Province Applicant before: Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences |
|
TA01 | Transfer of patent application right | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20220412 |