CN114317646A - I-type collagen biological fermentation process and intelligent fermentation manufacturing equipment thereof - Google Patents
I-type collagen biological fermentation process and intelligent fermentation manufacturing equipment thereof Download PDFInfo
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- CN114317646A CN114317646A CN202111460145.6A CN202111460145A CN114317646A CN 114317646 A CN114317646 A CN 114317646A CN 202111460145 A CN202111460145 A CN 202111460145A CN 114317646 A CN114317646 A CN 114317646A
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Abstract
The invention belongs to the field of biological fermentation, and particularly relates to an I-type collagen biological fermentation process and intelligent fermentation manufacturing equipment thereof, wherein the fermentation process comprises the following steps: inoculating the pichia pastoris strain liquid into a fermentation culture medium; after the carbon source is exhausted, the dissolved oxygen rises steeply, and glycerol is fed in a flowing manner; after the glycerol is exhausted, feeding methanol; collecting fermentation liquor, and centrifuging to obtain supernatant; when carrying out centrifugal separation to the zymotic fluid, solid sediment is piled up in the region that encloses between interior bounding wall and rotary drum lateral wall, the sediment is too much time-spent, the top of interior bounding wall is plugged up to the removal roof, then go up the shrouding and remove with lower shrouding, make logical groove open, the sediment outwards throws away via logical groove under the effect of centrifugal force, the function of clearing up is carried out to the sediment that produces in the geminate transistors rotary drum under the circumstances of not shutting down has been realized, work efficiency is improved, the interior bounding wall is plugged up through the roof, keep apart the sediment of interior bounding wall inner circle and the zymotic fluid of interior bounding wall top, the problem that the zymotic fluid also probably was discharged has been improved.
Description
Technical Field
The invention belongs to the field of biological fermentation, and particularly relates to an I-type collagen biological fermentation process and intelligent fermentation manufacturing equipment thereof.
Background
Type i collagen, the most abundant collagen in the human body, is widely used in the fields of surgical coagulation, bone tissue material manufacturing, whitening and health care, food protection, tanning industry and the like, and can be prepared by a process of biological fermentation, which is a process of converting raw materials into products required by human beings through a specific metabolic pathway under appropriate conditions by using microorganisms.
A Chinese patent with publication number CN105061589B discloses a recombinant human I-like collagen and a method for immobilized fermentation production thereof, wherein the method for the fermentation production comprises the steps of constructing an immobilized reaction system; inoculating the seed liquid inoculation amount of the pichia pastoris recombinant engineering bacteria into a common fermentation tank, and fixing yeast cells on cotton fibers in a fiber bed reactor; when the concentration of free bacteria in a common fermentation tank is constant and the substrate is not consumed any more, harvesting the culture medium for protein purification, and adding a fresh culture medium to start fermentation again; when the dissolved oxygen in the fermentation tank suddenly rises, beginning to add the glycerol in a flowing manner for about 24 hours, stopping adding the glycerol in a flowing manner, then rising the dissolved oxygen, beginning to add the methanol in a flowing manner, and leading the methanol to have induction time of 84 hours; replacing the fresh culture medium, and carrying out the next round of immobilized fermentation culture.
When handling the zymotic fluid after I type collagen biological fermentation, adopt tubular centrifuge to handle more, tubular centrifuge's theory of operation is: fermentation liquor enters from the center of the bottom of the rotary drum, under the action of centrifugal force, solids with high density are rapidly settled on the inner side of the rotary drum to form a solid layer, clarified liquid is lifted and discharged from the central axis of the rotary drum, when a general tubular centrifuge is used, frequent shutdown is needed, solid layer sediments in the rotary drum are taken out, frequent startup and shutdown not only reduces working efficiency, but also can influence the fermentation liquor in the machine, and the method is inconvenient to use.
Therefore, the invention provides an I-type collagen biological fermentation process and intelligent fermentation manufacturing equipment thereof.
Disclosure of Invention
To remedy the deficiencies of the prior art, at least one of the technical problems set forth in the background is addressed.
The technical scheme adopted by the invention for solving the technical problems is as follows: the invention relates to a type I collagen biological fermentation process, which comprises the following steps:
s1, inoculating the pichia pastoris strain liquid into a fermentation tank containing 5L of fermentation medium according to the inoculation amount of 11%, sterilizing the fermentation medium in advance, adjusting the fermentation temperature to 29 ℃, the initial stage, the tank pressure of the fermentation tank to 0.05MPa, adjusting the pH value of ammonia water to 4.7-4.9, and adjusting the rotating speed, the ventilation amount and the feeding speed to ensure that the dissolved oxygen is more than 34%;
s2, after the carbon source in the fermentation tank is exhausted, the dissolved oxygen rises suddenly, the glycerol feeding stage is started, 50% of glycerol is fed in a feeding mode to supplement the carbon source, the feeding amount of the glycerol is 95-155g/L, the feeding of the glycerol is stopped until the wet weight of the thalli is 216g/L, and the fermentation time is 19 h;
s3, after the glycerol in the fermentation tank is exhausted, the dissolved oxygen of the fermentation tank is more than 80%, the fermentation temperature is 28 ℃, the tank pressure is 0.06MPa, the pH value is adjusted to be 5.5-6.0 by ammonia water, the rotating speed and the ventilation volume are unchanged, the methanol flow acceleration is adjusted by dissolved oxygen feedback, 5-9% of glycerol is added into methanol, the dissolved oxygen is controlled to be 25-28%, and the methanol induction time is 85-89 h;
s4, after fermentation is finished, collecting fermentation liquor, and conveying the fermentation liquor into a rotary drum 2 of a tubular centrifuge through a liquor inlet 3 for centrifugal treatment to obtain supernatant;
s5, isolating solid sediments generated in the fermentation liquor centrifugation process through the inner surrounding plate 5 and the top plate 6 arranged in the tubular centrifuge, and discharging the sediments outwards through the through groove 10 and the discharge port 8 under the action of centrifugal force to achieve the function of automatic deslagging without stopping.
Preferably, the pichia pastoris is pichia pastoris, and the preservation number is CGMCC No. 5021.
Preferably, the formula of the fermentation medium is as follows: 85% H3PO418.2-23.6mL/L, CaSO4·2H2O is 1.03-1.12g/L, K2SO4Is 12.7 to 16.2g/L, MgSO4·7H2The concentration of 0 is 11.8-13.5g/L, KOH, the concentration of 2.8-3.9g/L, PTM1 solution is 3.6-4.1mL/L, and the concentration of glycerol is 20.2-30.0 g/L.
The utility model provides a I type collagen biofermentation intelligence fermentation preparation equipment, this preparation equipment is applicable to the aforesaid I type collagen biofermentation technology, which comprises a bod, the rotary drum is installed in the inside rotation of fuselage, and the head assembly of control rotary drum is installed on the top of fuselage, and the inside inlet of inserting the rotary drum is installed to the bottom of fuselage, and the outside of fuselage is provided with the liquid outlet, and the liquid outlet passes through catch tray and rotary drum intercommunication, and evenly distributed's logical groove is seted up to the lateral wall bottom of rotary drum, and fixed mounting has the interior bounding wall that surrounds logical groove on the interior diapire of rotary drum, and the top movable mounting of interior bounding wall has the roof, and the internally mounted of rotary drum blocks up last shrouding and lower shrouding that lead to the groove. When the device works, the top plate is separated from the inner surrounding plate in an initial state, and the top of the inner surrounding plate is open; fermentation liquor flows into the rotary drum through the liquid inlet, the starter head assembly drives the rotary drum to rotate, the fermentation liquor is subjected to centrifugal separation in the rotating process of the rotary drum, solid sediments are gradually settled at the bottom of the rotary drum, and supernate is gathered above the inside of the rotary drum and flows out through the liquid collecting disc and the liquid outlet; the through groove at the bottom end of the side wall of the rotary drum is blocked by the upper sealing plate and the lower sealing plate, so that solid sediments can be accumulated in an area enclosed between the inner enclosing plate and the side wall of the rotary drum, after the rotary drum works for a period of time, sediments are fully accumulated between the inner ring of the inner enclosing plate and the side wall of the rotary drum, when the sediments need to be cleaned, the top plate is started to move, the top end of the inner enclosing plate is blocked by the top plate, then the upper sealing plate and the lower sealing plate are started to move, the through groove is opened, and the sediments accumulated in the inner enclosing plate are thrown out outwards through the through groove under the action of centrifugal force, so that the function of cleaning the sediments generated in the rotary drum under the condition of no shutdown is realized, and the working efficiency is improved; in the process of slag removal and cleaning, the inner enclosing plate is plugged through the top plate, sediment at the inner ring of the inner enclosing plate is isolated from fermentation liquor above the inner enclosing plate, the rotary drum, the inner enclosing plate and the top plate still form a closed structure, the problem that the fermentation liquor is likely to be discharged in the process of slag removal is improved, and the use is more reliable.
Preferably, the top plate is movably inserted into the side wall of the rotary drum, a guide rod corresponding to the top plate is fixedly installed outside the rotary drum, the inner enclosing plate comprises a side plate and a slope plate, and a clamping groove corresponding to the top plate is formed in the inner wall of the slope plate. During operation, through setting up the stability that the roof was strengthened to the guide arm, the roof inserts in the draw-in groove on the interior bounding wall after inwards moving, and at this moment, between the lateral wall of roof, interior bounding wall, rotary drum and the diapire of rotary drum, form a sealed region, can discharge the sediment in this sealed region, and do not influence all the other zymotic fluids in the rotary drum and carry out centrifugal separation work.
Preferably, fixed mounting has evenly distributed's peripheral board on the lateral wall of rotary drum, and the internal diameter of peripheral board is the same with leading to the groove, has seted up the bin outlet on the lateral wall of peripheral board, and the size of bin outlet is greater than the size of lower shrouding, installs the switch door on the fuselage, goes up shrouding slidable mounting in the inside of peripheral board, and lower shrouding slides and pegs graft in the bottom of last shrouding, and lower shrouding is L type form. During operation, shrouding under the rebound, the sediment of interior bounding wall inner circle gets into the inside of outer surrounding plate from the bottom of shrouding down to outwards discharge the outside of rotary drum via the bin outlet, the staff opens the switch door, can collect the processing to discharged sediment.
Preferably, go up the shrouding and be close to one side fixed mounting of peripheral plate and have the extension board, fixed mounting has the driven piece that runs through to the extension board top on the lower shrouding, installs reset spring on the driven piece, goes up the shrouding and is close to one side of peripheral plate and is located the top fixed mounting of driven piece and has the diaphragm, and the bottom slidable mounting of diaphragm has the regulating plate that corresponds with the driven piece, and fixed mounting has the push pedal that corresponds with the regulating plate on the inner wall of peripheral plate. When the device works, in an initial state, the adjusting plate is extruded and supported above the driven block, so that the lower sealing plate extends out of the outer side of the upper sealing plate, and at the moment, the through groove is blocked by the upper sealing plate and the lower sealing plate; the sediment is to going up the shrouding and producing thrust with lower shrouding under the effect of centrifugal force, when piling up the sediment between the inner circle of interior bounding wall and the lateral wall of rotary drum, the centrifugal force grow that the sediment produced, shrouding and lower shrouding outwards move in the promotion, go up the shrouding and move the back outward, regulating plate and push pedal contact, the regulating plate is pushed by the push pedal and moves back and stagger with the driven piece, the driven piece is rebound under reset spring's effect, and then the shrouding shifts up under the drive, make the sediment outwards discharge through the bottom and the bin outlet of shrouding down, this process is driven by the centrifugal force that produces when the sediment of rotary drum inside becomes many, need not additionally to set up electric drive mechanism, moreover, the steam generator is simple in structure, and the use cost is reduced.
Preferably, the adjusting plate is provided with a supporting spring, and the bottom of the transverse plate is fixedly provided with a limiting block corresponding to the adjusting plate. During operation, the adjusting plate can slide in the fixed range of the bottom of the transverse plate, and the limiting block can ensure that the adjusting plate is extruded above the driven block in the initial state.
Preferably, the push plate is [ shaped ], a groove corresponding to the driven block is formed in the push plate, the adjusting plate comprises a slope section and a straight section, the straight section is aligned with the side edge of the push plate, the slope section is aligned with the driven block, and the top of the driven block is provided with a bevel edge aligned with the slope section. During operation, through this kind of setting, the action that the driven plate reciprocated and the action that the push pedal promoted the regulating plate to remove do not influence each other, shrouding and lower shrouding move to the rotary drum inboard in starting, and the regulating plate separates the back completely with the push pedal, and the regulating plate outwards moves in supporting spring's effect to extrude driven plate and lower shrouding through slope section and move down, make and go up the shrouding and lower shrouding and block up outer surrounding plate and logical groove sealing again, so that carry out the row's sediment clearance next time.
Preferably, the diaphragm extends to the one end fixed mounting in the peripheral board outside and has the planking, and the planking is the shape of falling L, connects through gear drive between planking and the roof, and the gear revolve installs in the outside of rotary drum, and the bottom of roof is provided with the rack section one with gear engaged with, and the top of planking is provided with the rack section two with gear engaged with, installs the release link on the planking, and the weight of roof is greater than the common weight of last shrouding, lower shrouding and planking. When the rotary drum rotates, the centrifugal force generated by the top plate is greater than the centrifugal force generated by the upper sealing plate, the lower sealing plate and the outer plate, so that the top plate extends outwards and drives the outer plate, the upper sealing plate and the lower sealing plate to move inwards through the gears, and the through grooves are blocked by the upper sealing plate and the lower sealing plate; when sediment is fully filled between the inner ring of the inner enclosing plate and the side wall of the rotary drum, the centrifugal force generated by the sediment is increased, the centrifugal force generated by the sediment, the upper sealing plate, the lower sealing plate and the outer plate is larger than that of the top plate, the upper sealing plate and the lower sealing plate move outwards, the top plate moves inwards at the same time, and the lower sealing plate always extends downwards to block the outer enclosing plate in the process that the top plate moves inwards to completely block the top of the inner enclosing plate; after the top plate is inserted into the clamping groove to seal the top of the inner enclosing plate, the adjusting plate is contacted with the push plate, the adjusting plate is extruded by the push plate to move back and is staggered with the driven block, and the driven block drives the lower enclosing plate to move upwards under the action of the reset spring, so that sediment can be discharged outwards through the bottom of the lower enclosing plate and the discharge port; after the sediment of interior bounding wall inner circle was discharged, sediment thrust that goes up the shrouding and receive down the shrouding diminishes or disappears, the sediment, go up the shrouding, the produced centrifugal force of lower shrouding and planking is less than the centrifugal force of roof, the roof outwards removes, the integrated device resumes initial condition once more, so, can circulate and carry out work, the device utilizes the centrifugal force that the sediment produced when piling up as the power supply, carry out automatic sediment work of arranging, need not to shut down the operation, also need not additionally to set up electric drive mechanism, when having improved work efficiency, use cost is reduced
The invention has the following beneficial effects:
1. according to the I-type collagen biofermentation intelligent fermentation manufacturing equipment, when fermentation liquor is centrifugally separated, solid sediments are accumulated in an area enclosed between the inner enclosing plate and the side wall of the rotary drum, when the sediments are excessive, the top plate is moved to block the top end of the inner enclosing plate, then the upper sealing plate and the lower sealing plate are moved to open the through groove, and the sediments accumulated in the inner enclosing plate are thrown out outwards through the through groove under the action of centrifugal force, so that the function of cleaning sediments generated in the rotary drum under the condition of no power failure is realized, the working efficiency is improved, the inner enclosing plate is blocked by the top plate, the sediments in the inner ring of the inner enclosing plate are separated from the fermentation liquor above the inner enclosing plate, the problem that the fermentation liquor can be discharged when the sediments are cleaned is solved, and the use is more reliable.
2. The invention relates to a type I collagen biological fermentation process and intelligent fermentation manufacturing equipment thereof. The weight of roof is greater than the weight of last shrouding, lower shrouding and planking, and when the sediment was piled up, caused extra centrifugal force to last shrouding and lower shrouding, promoted roof, last shrouding and lower shrouding and removed, the device uses the centrifugal force that the sediment pile back produced as the power supply, carries out automatic sediment work of arranging, need not to shut down the operation, also need not additionally to set up electric drive mechanism, when having improved work efficiency, has reduced use cost.
Drawings
The invention will be further explained with reference to the drawings.
FIG. 1 is a perspective view of the present invention;
FIG. 2 is a front cross-sectional view of the present invention;
FIG. 3 is a partial cross-sectional view taken at section A-A of FIG. 2 in accordance with the present invention;
FIG. 4 is an enlarged view of portion B of FIG. 2 in accordance with the present invention;
FIG. 5 is a exploded view of the inside and outside peripheral panel attachment structure of the present invention;
FIG. 6 is a perspective view of a push plate and an adjustment plate of the present invention;
FIG. 7 is a schematic view of a through slot of the present invention;
in the figure: 1. a body; 101. a head assembly; 2. a drum; 3. a liquid inlet; 4. a liquid outlet; 5. an inner coaming; 501. a card slot; 6. a top plate; 601. a guide bar; 7. a peripheral plate; 8. a discharge outlet; 9. opening and closing the door; 10. a through groove; 11. an upper sealing plate; 12. a lower sealing plate; 13. a support plate; 14. a driven block; 15. a transverse plate; 16. an adjusting plate; 17. pushing the plate; 18. an outer plate; 19. a gear; 20. a first rack segment; 21. a rack section II; 22. a reset rod.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
The invention relates to a type I collagen biological fermentation process, which comprises the following steps:
s1, inoculating the pichia pastoris strain liquid into a fermentation tank containing 5L of fermentation medium according to the inoculation amount of 11%, sterilizing the fermentation medium in advance, adjusting the fermentation temperature to 29 ℃, the initial stage, the tank pressure of the fermentation tank to 0.05MPa, adjusting the pH value of ammonia water to 4.7-4.9, and adjusting the rotating speed, the ventilation amount and the feeding speed to ensure that the dissolved oxygen is more than 34%;
s2, after the carbon source in the fermentation tank is exhausted, the dissolved oxygen rises suddenly, the glycerol feeding stage is started, 50% of glycerol is fed in a feeding mode to supplement the carbon source, the feeding amount of the glycerol is 95-155g/L, the feeding of the glycerol is stopped until the wet weight of the thalli is 216g/L, and the fermentation time is 19 h;
s3, after the glycerol in the fermentation tank is exhausted, the dissolved oxygen of the fermentation tank is more than 80%, the fermentation temperature is 28 ℃, the tank pressure is 0.06MPa, the pH value is adjusted to be 5.5-6.0 by ammonia water, the rotating speed and the ventilation volume are unchanged, the methanol flow acceleration is adjusted by dissolved oxygen feedback, 5-9% of glycerol is added into methanol, the dissolved oxygen is controlled to be 25-28%, and the methanol induction time is 85-89 h;
s4, after fermentation is finished, collecting fermentation liquor, and conveying the fermentation liquor into a rotary drum 2 of a tubular centrifuge through a liquor inlet 3 for centrifugal treatment to obtain supernatant;
s5, isolating solid sediments generated in the fermentation liquor centrifugation process through the inner surrounding plate 5 and the top plate 6 arranged in the tubular centrifuge, and discharging the sediments outwards through the through groove 10 and the discharge port 8 under the action of centrifugal force to achieve the function of automatic deslagging without stopping.
The pichia pastoris is pichia pastoris, and the preservation number is CGMCC No. 5021.
The formula of the fermentation medium is as follows: 85% H3PO418.2-23.6mL/L, CaSO4·2H2O is 1.03-1.12g/L, K2SO4Is 12.7 to 16.2g/L, MgSO4·7H2The concentration of 0 is 11.8-13.5g/L, KOH, the concentration of 2.8-3.9g/L, PTM1 solution is 3.6-4.1mL/L, and the concentration of glycerol is 20.2-30.0 g/L.
Example one
The formula of the fermentation medium is as follows: 85% H3PO4Is 18.2mL/L, CaSO4·2H2O is 1.03g/L, K2SO4Is 12.7g/L, MgSO4·7H2The solution is 3.6mL/L when the concentration of 0 is 11.8g/L, KOH and the concentration of 2.8g/L, PTM1 and the concentration of glycerol is 20.2 g/L;
inoculating 11% of the Pichia pastoris strain liquid into a fermentation tank, wherein the fermentation temperature is 29 ℃, the initial stirring speed is 200rpm, the initial tank pressure is 0.05MPa, the pH is controlled to be 4.7 in the fermentation process, and the rotating speed, the ventilation quantity and the material supplementing speed are adjusted to ensure that the dissolved oxygen is more than 34%; after the carbon source is exhausted, feeding 50% of glycerol in a feeding amount of 95g/L, stopping feeding the glycerol until the wet weight of the thalli is 216g/L, and at the moment, fermenting for 19 hours; after the glycerol is exhausted, entering a methanol induction stage, controlling the fermentation temperature to be 28 ℃, the tank pressure to be 0.06MPa, controlling the pH to be 5.5 in the fermentation process, keeping the rotating speed and the ventilation constant, adjusting the methanol flow acceleration through dissolved oxygen feedback, and adding 5-9% of glycerol into the methanol to control the dissolved oxygen to be 25%, wherein the methanol induction time is 85 h; after fermentation is finished, collecting fermentation liquor, and centrifuging the fermentation liquor to obtain supernatant; HPLC detection shows that the final type I collagen concentration is 18.1g/L, the fermentation period is 104h, and the fermentation production level is 0.174 g/L.h.
Example two
The formula of the fermentation medium is as follows: 85% H3PO4Is 21.5mL/L, CaSO4·2H2O is 1.07g/L, K2SO4Is 14.5g/L, MgSO4·7H20 is 12.5g/L, KOH, 3.3g/L, PTM1 solution is 3.9mL/L, and glycerol is 25.2 g/L;
inoculating 11% of the Pichia pastoris strain liquid into a fermentation tank, wherein the fermentation temperature is 29 ℃, the initial stirring speed is 200rpm, the initial tank pressure is 0.05MPa, the pH is controlled to be 4.8 in the fermentation process, and the rotating speed, the ventilation quantity and the material supplementing speed are adjusted to ensure that the dissolved oxygen is more than 34%; after the carbon source is exhausted, feeding 50% of glycerol in an amount of 120g/L, stopping feeding the glycerol until the wet weight of the thalli is 216g/L, and at the moment, fermenting for 19 hours; after the glycerol is exhausted, entering a methanol induction stage, controlling the fermentation temperature to be 28 ℃, the tank pressure to be 0.06MPa, controlling the pH to be 5.7 in the fermentation process, keeping the rotating speed and the ventilation constant, adjusting the methanol flow acceleration through dissolved oxygen feedback, and adding 5-9% of glycerol into the methanol to control the dissolved oxygen to be 27%, wherein the methanol induction time is 87 h; after fermentation is finished, collecting fermentation liquor, and centrifuging the fermentation liquor to obtain supernatant; HPLC detection shows that the final type I collagen concentration is 17.1g/L, the fermentation period is 106h, and the fermentation production level is 0.161 g/L.h.
EXAMPLE III
The formula of the fermentation medium is as follows: 85% H3PO4Is 23.6mL/L, CaSO4·2H2O is 1.12g/L, K2SO4Is 16.2g/L, MgSO4·7H20 is 13.5g/L, KOH, 3.9g/L, PTM1 solution is 4.1mL/L, and glycerol is 30.0 g/L;
inoculating 11% of the Pichia pastoris strain liquid into a fermentation tank, wherein the fermentation temperature is 29 ℃, the initial stirring speed is 200rpm, the initial tank pressure is 0.05MPa, the pH is controlled to be 4.9 in the fermentation process, and the rotating speed, the ventilation quantity and the material supplementing speed are adjusted to ensure that the dissolved oxygen is more than 34%; after the carbon source is exhausted, feeding 50% of glycerol in an amount of 155g/L, and stopping feeding the glycerol when the wet weight of the thalli is 216g/L, wherein the fermentation time is 19 h; after the glycerol is exhausted, entering a methanol induction stage, controlling the fermentation temperature to be 28 ℃, the tank pressure to be 0.06MPa, controlling the pH to be 6.0 in the fermentation process, keeping the rotating speed and the ventilation quantity unchanged, adjusting the methanol flow acceleration through dissolved oxygen feedback, and adding 5-9% of glycerol into the methanol to control the dissolved oxygen to be 28%, wherein the methanol induction time is 89 h; after fermentation is finished, collecting fermentation liquor, and centrifuging the fermentation liquor to obtain supernatant; HPLC detection shows that the final type I collagen concentration is 20.1g/L, the fermentation period is 108h, and the fermentation production level is 0.186 g/L.h.
In the embodiment of the invention, in order to further improve the working efficiency of the type I collagen biological fermentation, the equipment adopted in the process is improved, and the method specifically comprises the following steps:
as shown in fig. 1 to 4, a I type collagen biofermentation intelligence fermentation preparation equipment, this preparation equipment is applicable to the aforesaid I type collagen biofermentation technology, including fuselage 1, rotary drum 2 is installed in the inside rotation of fuselage 1, the head assembly 101 of control rotary drum 2 is installed on the top of fuselage 1, the inside inlet 3 of inserting rotary drum 2 is installed to the bottom of fuselage 1, the outside of fuselage 1 is provided with liquid outlet 4, liquid outlet 4 passes through catch tray and rotary drum 2 intercommunication, evenly distributed's logical groove 10 is seted up to the lateral wall bottom of rotary drum 2, fixed mounting has the interior bounding wall 5 that surrounds logical groove 10 on rotary drum 2's the interior diapire, the top movable mounting of interior bounding wall 5 has roof 6, the internally mounted of rotary drum 2 has the last shrouding 11 and lower shrouding 12 of blocking logical groove 10. When the device works, in an initial state, the top plate 6 is separated from the inner surrounding plate 5, and the top of the inner surrounding plate 5 is open; fermentation liquor flows into the rotary drum 2 through the liquid inlet 3, the starter head assembly 101 drives the rotary drum 2 to rotate, centrifugal separation is carried out on the fermentation liquor in the rotating process of the rotary drum 2, solid sediments are gradually settled at the bottom of the rotary drum 2, and supernate is gathered above the inner part of the rotary drum 2 and flows out through the liquid collecting disc and the liquid outlet 4; because the through groove 10 at the bottom end of the side wall of the rotary drum 2 is blocked by the upper closing plate 11 and the lower closing plate 12, solid sediments can be accumulated in an area enclosed between the inner enclosing plate 5 and the side wall of the rotary drum 2, after the rotary drum 2 works for a period of time, sediments are fully accumulated between the inner ring of the inner enclosing plate 5 and the side wall of the rotary drum 2, when the sediments need to be cleaned, the top plate 6 is started to move, the top end of the inner enclosing plate 5 is blocked by the top plate 6, then the upper closing plate 11 and the lower closing plate 12 are started to move, so that the through groove 10 is opened, the sediments accumulated in the inner enclosing plate 5 are thrown out through the through groove 10 under the action of centrifugal force, the function of cleaning the sediments generated in the rotary drum 2 without stopping the machine is realized, and the working efficiency is improved; in the process of slag discharging and cleaning, the inner surrounding plate 5 is blocked through the top plate 6, sediment at the inner ring of the inner surrounding plate 5 is isolated from fermentation liquor above the inner surrounding plate 5, the rotary drum 2, the inner surrounding plate 5 and the top plate 6 still form a closed structure, the problem that the fermentation liquor can be discharged in the process of cleaning the sediment is improved, and the use is more reliable.
As shown in fig. 4 to 5, the top plate 6 is movably inserted into the side wall of the rotary drum 2, a guide rod 601 corresponding to the top plate 6 is fixedly installed outside the rotary drum 2, the inner enclosure plate 5 includes a side plate and a slope plate, and a slot 501 corresponding to the top plate 6 is formed on the inner wall of the slope plate. During operation, the guide rod 601 is arranged to enhance the stability of the top plate 6, the top plate 6 moves inwards and then is inserted into the clamping groove 501 in the inner surrounding plate 5, at the moment, a sealed area is formed among the top plate 6, the inner surrounding plate 5, the side wall of the rotary drum 2 and the bottom wall of the rotary drum 2, sediment in the sealed area can be discharged, and the centrifugal separation work of the rest fermentation liquor in the rotary drum 2 is not influenced.
As shown in fig. 3 to 7, peripheral plate 7 that evenly distributed is fixed to the lateral wall of rotary drum 2, the internal diameter of peripheral plate 7 is the same with logical groove 10, has seted up bin outlet 8 on the lateral wall of peripheral plate 7, and the size of bin outlet 8 is greater than the size of lower shrouding 12, installs switch door 9 on the fuselage 1, goes up shrouding 11 slidable mounting in the inside of peripheral plate 7, and lower shrouding 12 slides and pegs graft in the bottom of last shrouding 11, and lower shrouding 12 is the L type form. When the device works, the lower sealing plate 12 is moved upwards, sediment in the inner ring of the inner sealing plate 5 enters the inner part of the outer peripheral plate 7 from the bottom of the lower sealing plate 12 and is discharged to the outer side of the rotary drum 2 through the discharge port 8, and a worker opens the switch door 9 to collect and process the discharged sediment.
As shown in fig. 4 to 5, a support plate 13 is fixedly mounted on one side of the upper sealing plate 11 close to the peripheral plate 7, a driven block 14 penetrating above the support plate 13 is fixedly mounted on the lower sealing plate 12, a return spring is mounted on the driven block 14, a transverse plate 15 is fixedly mounted on one side of the upper sealing plate 11 close to the peripheral plate 7 and above the driven block 14, an adjusting plate 16 corresponding to the driven block 14 is slidably mounted at the bottom of the transverse plate 15, and a push plate 17 corresponding to the adjusting plate 16 is fixedly mounted on the inner wall of the peripheral plate 7. During operation, in an initial state, the adjusting plate 16 is pressed and abutted above the driven block 14, so that the lower sealing plate 12 extends out of the upper sealing plate 11, and at the same time, the through groove 10 is blocked by the upper sealing plate 11 and the lower sealing plate 12; the sediment is to going up shrouding 11 and lower shrouding 12 production thrust under the effect of centrifugal force, when piling up the sediment between the inner circle of interior bounding wall 5 and the lateral wall of rotary drum 2, the centrifugal force grow that the sediment produced, shrouding 11 and lower shrouding 12 outwards move in the promotion, go up shrouding 11 and move back outward, regulating plate 16 and push pedal 17 contact, regulating plate 16 is pushed by push pedal 17 and moves back and stagger with driven piece 14, driven piece 14 rebound under reset spring's effect, and then move up shrouding 12 under the drive, make the sediment can drive through the bottom and the discharge gate 8 of shrouding 12 outwards discharge down, this process is driven by the centrifugal force that produces when the sediment of rotary drum 2 inside becomes many, need not additionally to set up electric drive mechanism, moreover, the steam generator is simple in structure, and the use cost is reduced.
As shown in fig. 4 to 5, the adjusting plate 16 is provided with a supporting spring, and the bottom of the horizontal plate 15 is fixedly provided with a limiting block corresponding to the adjusting plate 16. During operation, the adjusting plate 16 can slide in the fixed range of the bottom of the transverse plate 15, and the limiting block can ensure that the adjusting plate 16 is extruded above the driven block 14 in the initial state.
As shown in fig. 6, the push plate 17 is in a shape of a 'shape', a groove corresponding to the driven block 14 is formed in the push plate 17, the adjusting plate 16 includes a slope section and a straight section, the straight section is aligned with a side edge of the push plate 17, the slope section is aligned with the driven block 14, and a bevel edge aligned with the slope section is formed at the top of the driven block 14. During operation, through this kind of setting, the action that moves from the piece 14 and the action that the push pedal 17 promoted regulating plate 16 to remove do not influence each other, shrouding 11 and lower shrouding 12 move to rotary drum 2 inboard in starting, and regulating plate 16 separates the back with the push pedal 17 completely, regulating plate 16 outwards moves in supporting spring's effect, and extrude driven piece 14 and lower shrouding 12 through the slope section and move down, make and go up shrouding 11 and lower shrouding 12 and block up peripheral plate 7 and logical groove 10 again, so that carry out the row's of next time sediment clearance.
As shown in fig. 4 to 5, an outer plate 18 is fixedly mounted at one end of the transverse plate 15 extending to the outer side of the peripheral plate 7, the outer plate 18 is in an inverted L shape, the outer plate 18 and the top plate 6 are in transmission connection through a gear 19, the gear 19 is rotatably mounted at the outer side of the rotary drum 2, a first rack segment 20 meshed with the gear 19 is arranged at the bottom of the top plate 6, a second rack segment 21 meshed with the gear 19 is arranged at the top of the outer plate 18, a reset rod 22 is mounted on the outer plate 18, and the weight of the top plate 6 is greater than the common weight of the upper sealing plate 11, the lower sealing plate 12 and the outer plate 18. When the rotary drum 2 rotates, the centrifugal force generated by the top plate 6 is larger than the centrifugal forces generated by the upper sealing plate 11, the lower sealing plate 12 and the outer plate 18, so that the top plate 6 extends outwards and drives the outer plate 18, the upper sealing plate 11 and the lower sealing plate 12 to move inwards through the gear 19, and the through groove 10 is blocked by the upper sealing plate 11 and the lower sealing plate 12; when sediment is fully piled between the inner ring of the inner surrounding plate 5 and the side wall of the rotary drum 2, the centrifugal force generated by the sediment is increased, the centrifugal force generated by the sediment, the upper sealing plate 11, the lower sealing plate 12 and the outer plate 18 is larger than that of the top plate 6, the upper sealing plate 11 and the lower sealing plate 12 move outwards, the top plate 6 moves inwards, and the lower sealing plate 12 always extends downwards to seal and seal the outer surrounding plate 7 in the process that the top plate 6 moves inwards to completely seal and seal the top of the inner surrounding plate 5; after the top plate 6 is inserted into the clamping groove 501 to seal the top of the inner enclosing plate 5, the adjusting plate 16 is in contact with the push plate 17, the adjusting plate 16 is extruded by the push plate 17 to move back and is staggered with the driven block 14, and the driven block 14 drives the lower enclosing plate 12 to move upwards under the action of the return spring, so that sediment can be discharged outwards through the bottom of the lower enclosing plate 12 and the discharge port 8; the sediment of 5 inner circles of interior bounding walls is discharged the back, the sediment thrust that goes up shrouding 11 and lower shrouding 12 and receive diminishes or disappears, the sediment, go up shrouding 11, the produced centrifugal force of lower shrouding 12 and planking 18 is less than the centrifugal force of roof 6, roof 6 outwards removes, the integrated device resumes initial condition once more, so, can circulate and carry out work, the device utilizes the centrifugal force that the sediment produced when piling up as the power supply, carry out automatic sediment work of arranging, need not to shut down the operation, also need not additionally to set up electric drive mechanism, when having improved work efficiency, use cost is reduced.
The working principle is as follows:
in an initial state, the top plate 6 is separated from the inner surrounding plate 5, and the top of the inner surrounding plate 5 is in an open shape; fermentation liquor flows into the rotary drum 2 through the liquid inlet 3, the starter head assembly 101 drives the rotary drum 2 to rotate, centrifugal separation is carried out on the fermentation liquor in the rotating process of the rotary drum 2, solid sediments are gradually settled at the bottom of the rotary drum 2, and supernate is gathered above the inner part of the rotary drum 2 and flows out through the liquid collecting disc and the liquid outlet 4; because the through groove 10 at the bottom end of the side wall of the rotary drum 2 is blocked by the upper closing plate 11 and the lower closing plate 12, solid sediments can be accumulated in an area enclosed between the inner enclosing plate 5 and the side wall of the rotary drum 2, after the rotary drum 2 works for a period of time, sediments are fully accumulated between the inner ring of the inner enclosing plate 5 and the side wall of the rotary drum 2, when the sediments need to be cleaned, the top plate 6 is started to move, the top end of the inner enclosing plate 5 is blocked by the top plate 6, then the upper closing plate 11 and the lower closing plate 12 are started to move, so that the through groove 10 is opened, the sediments accumulated in the inner enclosing plate 5 are thrown out through the through groove 10 under the action of centrifugal force, the function of cleaning the sediments generated in the rotary drum 2 without stopping the machine is realized, and the working efficiency is improved; in the process of slag discharging and cleaning, the inner surrounding plate 5 is blocked through the top plate 6, sediment at the inner ring of the inner surrounding plate 5 is isolated from fermentation liquor above the inner surrounding plate 5, the rotary drum 2, the inner surrounding plate 5 and the top plate 6 still form a closed structure, the problem that the fermentation liquor can be discharged in the process of cleaning the sediment is improved, and the use is more reliable.
Through setting up the stability that guide arm 601 strengthened roof 6, in roof 6 inserted draw-in groove 501 on interior bounding wall 5 after the inward movement, at this moment, between roof 6, interior bounding wall 5, the lateral wall of rotary drum 2 and the diapire of rotary drum 2, formed a sealed region, can discharge the sediment in this sealed region, and do not influence the remaining zymotic fluid in the rotary drum 2 and carry out centrifugal separation work.
The lower closing plate 12 is moved upwards, sediment at the inner ring of the inner enclosing plate 5 enters the inner part of the outer enclosing plate 7 from the bottom of the lower closing plate 12 and is discharged to the outer side of the rotary drum 2 through the discharge opening 8, and a worker opens the switch door 9 to collect and process the discharged sediment.
In an initial state, the adjusting plate 16 is pressed and abutted above the driven block 14, so that the lower sealing plate 12 extends out of the upper sealing plate 11, and at the same time, the through groove 10 is sealed by the upper sealing plate 11 and the lower sealing plate 12; the sediment is to going up shrouding 11 and lower shrouding 12 production thrust under the effect of centrifugal force, when piling up the sediment between the inner circle of interior bounding wall 5 and the lateral wall of rotary drum 2, the centrifugal force grow that the sediment produced, shrouding 11 and lower shrouding 12 outwards move in the promotion, go up shrouding 11 and move back outward, regulating plate 16 and push pedal 17 contact, regulating plate 16 is pushed by push pedal 17 and moves back and stagger with driven piece 14, driven piece 14 rebound under reset spring's effect, and then move up shrouding 12 under the drive, make the sediment can drive through the bottom and the discharge gate 8 of shrouding 12 outwards discharge down, this process is driven by the centrifugal force that produces when the sediment of rotary drum 2 inside becomes many, need not additionally to set up electric drive mechanism, moreover, the steam generator is simple in structure, and the use cost is reduced.
The action that the driven plate 14 reciprocated and the action that push pedal 17 promoted regulating plate 16 to remove do not influence each other, shrouding 11 and lower shrouding 12 move to the rotary drum 2 inboard on starting, and regulating plate 16 separates the back with push pedal 17 completely, and regulating plate 16 outwards moves in supporting spring's effect to extrude driven plate 14 and lower shrouding 12 through the slope section and move down, make and go up shrouding 11 and lower shrouding 12 and block up peripheral plate 7 and logical groove 10 again, so that carry out the row's of next time sediment clearance.
According to centrifugationForce formula
(F: centrifugal force, m: mass of the object, r: radius of circular motion of the object, v: speed of circular motion of the object), knowing that the magnitude of the centrifugal force is related to the mass of the object, when the rotary drum 2 rotates, the centrifugal force generated by the top plate 6 is greater than the centrifugal force generated by the upper sealing plate 11, the lower sealing plate 12 and the outer plate 18, therefore, the top plate 6 extends outwards, and the outer plate 18, the upper sealing plate 11 and the lower sealing plate 12 are driven to move inwards through the gear 19, so that the upper sealing plate 11 and the lower sealing plate 12 block the through groove 10; when sediment is fully piled between the inner ring of the inner surrounding plate 5 and the side wall of the rotary drum 2, the centrifugal force generated by the sediment is increased, the centrifugal force generated by the sediment, the upper sealing plate 11, the lower sealing plate 12 and the outer plate 18 is larger than that of the top plate 6, the upper sealing plate 11 and the lower sealing plate 12 move outwards, the top plate 6 moves inwards, and the lower sealing plate 12 always extends downwards to seal and seal the outer surrounding plate 7 in the process that the top plate 6 moves inwards to completely seal and seal the top of the inner surrounding plate 5; after the top plate 6 is inserted into the clamping groove 501 to seal the top of the inner enclosing plate 5, the adjusting plate 16 is in contact with the push plate 17, the adjusting plate 16 is extruded by the push plate 17 to move back and is staggered with the driven block 14, and the driven block 14 drives the lower enclosing plate 12 to move upwards under the action of the return spring, so that sediment can be discharged outwards through the bottom of the lower enclosing plate 12 and the discharge port 8; the sediment of 5 inner circles of interior bounding walls is discharged the back, the sediment thrust that goes up shrouding 11 and lower shrouding 12 and receive diminishes or disappears, the sediment, go up shrouding 11, the produced centrifugal force of lower shrouding 12 and planking 18 is less than the centrifugal force of roof 6, roof 6 outwards removes, the integrated device resumes initial condition once more, so, can circulate and carry out work, the device utilizes the centrifugal force that the sediment produced when piling up as the power supply, carry out automatic sediment work of arranging, need not to shut down the operation, also need not additionally to set up electric drive mechanism, when having improved work efficiency, use cost is reduced.
The front, the back, the left, the right, the upper and the lower are all based on figure 1 in the attached drawings of the specification, according to the standard of the observation angle of a person, the side of the device facing an observer is defined as the front, the left side of the observer is defined as the left, and the like.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are used merely for convenience in describing the present invention and for simplifying the description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the scope of the present invention.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. A type I collagen biological fermentation process is characterized in that: the method comprises the following steps:
s1, inoculating the pichia pastoris strain liquid into a fermentation tank containing 5L of fermentation medium according to the inoculation amount of 11%, sterilizing the fermentation medium in advance, adjusting the fermentation temperature to 29 ℃, the initial stage, the tank pressure of the fermentation tank to 0.05MPa, adjusting the pH value of ammonia water to 4.7-4.9, and adjusting the rotating speed, the ventilation amount and the feeding speed to ensure that the dissolved oxygen is more than 34%;
s2, after the carbon source in the fermentation tank is exhausted, the dissolved oxygen rises suddenly, the glycerol feeding stage is started, 50% of glycerol is fed in a feeding mode to supplement the carbon source, the feeding amount of the glycerol is 95-155g/L, the feeding of the glycerol is stopped until the wet weight of the thalli is 216g/L, and the fermentation time is 19 h;
s3, after the glycerol in the fermentation tank is exhausted, the dissolved oxygen of the fermentation tank is more than 80%, the fermentation temperature is 28 ℃, the tank pressure is 0.06MPa, the pH value is adjusted to be 5.5-6.0 by ammonia water, the rotating speed and the ventilation volume are unchanged, the methanol flow acceleration is adjusted by dissolved oxygen feedback, 5-9% of glycerol is added into methanol, the dissolved oxygen is controlled to be 25-28%, and the methanol induction time is 85-89 h;
s4, after fermentation is finished, collecting fermentation liquor, and conveying the fermentation liquor into a rotary drum (2) of a tubular centrifuge through a liquid inlet (3) for centrifugal treatment to obtain supernatant;
s5, isolating solid sediments generated in the fermentation liquor centrifugation process through an inner surrounding plate (5) and a top plate (6) arranged in the tubular centrifuge, and discharging the sediments outwards through a through groove (10) and a discharge hole (8) under the action of centrifugal force to achieve the function of automatic deslagging without stopping.
2. The type I collagen biofermentation process according to claim 1, wherein: the pichia pastoris is pichia pastoris, and the preservation number is CGMCC No. 5021.
3. The type I collagen biofermentation process according to claim 1, wherein: the formula of the fermentation medium is as follows: 85% H3PO418.2-23.6mL/L, CaSO4·2H2O is 1.03-1.12g/L, K2SO4Is 12.7 to 16.2g/L, MgSO4·7H2The concentration of 0 is 11.8-13.5g/L, KOH, the concentration of 2.8-3.9g/L, PTM1 solution is 3.6-4.1mL/L, and the concentration of glycerol is 20.2-30.0 g/L.
4. An intelligent fermentation manufacturing device for type I collagen biofermentation, which is suitable for the type I collagen biofermentation process as claimed in any one of claims 1-3, and is characterized in that: including fuselage (1), rotary drum (2) are installed in the inside rotation of fuselage (1), head assembly (101) of control rotary drum (2) are installed on the top of fuselage (1), inlet (3) inside inserting rotary drum (2) are installed to the bottom of fuselage (1), the outside of fuselage (1) is provided with liquid outlet (4), liquid outlet (4) are through catch basin and rotary drum (2) intercommunication, evenly distributed's logical groove (10) are seted up to the lateral wall bottom of rotary drum (2), fixed mounting has interior bounding wall (5) that surround logical groove (10) on the interior diapire of rotary drum (2), the top movable mounting of interior bounding wall (5) has roof (6), the internally mounted of rotary drum (2) has last shrouding (11) and lower shrouding (12) of plugging up logical groove (10).
5. The intelligent fermentation manufacturing equipment for type I collagen biological fermentation according to claim 4, characterized in that: the top plate (6) is movably inserted into the side wall of the rotary drum (2), the outer portion of the rotary drum (2) is fixedly provided with a guide rod (601) corresponding to the top plate (6), the inner enclosing plate (5) comprises a side plate and a slope plate, and the inner wall of the slope plate is provided with a clamping groove (501) corresponding to the top plate (6).
6. The intelligent fermentation manufacturing equipment for type I collagen biological fermentation according to claim 4, characterized in that: fixed mounting has evenly distributed's peripheral board (7) on the lateral wall of rotary drum (2), the internal diameter of peripheral board (7) is the same with leading to groove (10), bin outlet (8) have been seted up on the lateral wall of peripheral board (7), the size of bin outlet (8) is greater than down the size of shrouding (12), install switch door (9) on fuselage (1), go up shrouding (11) slidable mounting in the inside of peripheral board (7), shrouding (12) slide grafting is in the bottom of last shrouding (11) down, shrouding (12) are L type form down.
7. The intelligent fermentation manufacturing equipment for type I collagen biological fermentation according to claim 6, characterized in that: go up shrouding (11) and be close to one side fixed mounting of peripheral plate (7) have extension board (13), fixed mounting has driven piece (14) that run through to extension board (13) top down on shrouding (12), install reset spring on driven piece (14), it has diaphragm (15) to go up shrouding (11) and be located the top fixed mounting of driven piece (14) near one side of peripheral plate (7), the bottom slidable mounting of diaphragm (15) has regulating plate (16) that correspond with driven piece (14), fixed mounting has push pedal (17) that correspond with regulating plate (16) on the inner wall of peripheral plate (7).
8. The intelligent fermentation manufacturing equipment for type I collagen biological fermentation according to claim 7, characterized in that: a supporting spring is installed on the adjusting plate (16), and a limiting block corresponding to the adjusting plate (16) is fixedly installed at the bottom of the transverse plate (15).
9. The intelligent fermentation manufacturing equipment for type I collagen biological fermentation according to claim 7, characterized in that: the push plate (17) is in a shape of a 'shape', a groove corresponding to the driven block (14) is formed in the push plate (17), the adjusting plate (16) comprises a slope section and a straight section, the straight section is aligned with the side edge of the push plate (17), the slope section is aligned with the driven block (14), and a bevel edge aligned with the slope section is arranged at the top of the driven block (14).
10. The intelligent fermentation manufacturing equipment for type I collagen biological fermentation according to claim 7, characterized in that: the outer plate (18) is fixedly mounted at one end, extending to the outer side of the peripheral plate (7), of the transverse plate (15), the outer plate (18) is in an inverted L shape, the outer plate (18) is in transmission connection with the top plate (6) through a gear (19), the gear (19) is rotatably mounted on the outer side of the rotary drum (2), a first rack section (20) meshed with the gear (19) is arranged at the bottom of the top plate (6), a second rack section (21) meshed with the gear (19) is arranged at the top of the outer plate (18), a reset rod (22) is mounted on the outer plate (18), and the weight of the top plate (6) is larger than the common weight of the upper sealing plate (11), the lower sealing plate (12) and the outer plate (18).
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| CN102443057A (en) * | 2011-10-26 | 2012-05-09 | 南京理工大学 | Recombinant humanized collagen and its preparation method |
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| CN112827668A (en) * | 2020-12-31 | 2021-05-25 | 青岛诺凯达机械制造有限公司 | Butterfly centrifuge |
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2021
- 2021-12-02 CN CN202111460145.6A patent/CN114317646B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102443057A (en) * | 2011-10-26 | 2012-05-09 | 南京理工大学 | Recombinant humanized collagen and its preparation method |
| CN102527524A (en) * | 2012-01-19 | 2012-07-04 | 青岛诺凯达机械制造有限公司 | Automatic slag-discharging tube-type separator |
| CN103691581A (en) * | 2013-12-17 | 2014-04-02 | 青岛诺凯达机械制造有限公司 | Tubular centrifuge drum and control method |
| CN205323995U (en) * | 2016-01-16 | 2016-06-22 | 辽阳市太子河区天兴制药机械设备厂 | Can arrange tubular bowl centrifuge of sediment in succession |
| CN106191181A (en) * | 2016-07-22 | 2016-12-07 | 江苏江山聚源生物技术有限公司 | A kind of fermentation technology of Pichia anomala expression recombiant protein |
| CN112827668A (en) * | 2020-12-31 | 2021-05-25 | 青岛诺凯达机械制造有限公司 | Butterfly centrifuge |
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