CN114317252A - Heating device applied to nucleic acid extractor and nucleic acid extractor - Google Patents
Heating device applied to nucleic acid extractor and nucleic acid extractor Download PDFInfo
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- CN114317252A CN114317252A CN202111651526.2A CN202111651526A CN114317252A CN 114317252 A CN114317252 A CN 114317252A CN 202111651526 A CN202111651526 A CN 202111651526A CN 114317252 A CN114317252 A CN 114317252A
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Abstract
The invention relates to a heating device applied to a nucleic acid extractor and the nucleic acid extractor, comprising a heating plate, wherein the heating plate is provided with a plurality of heating grooves, and the heating grooves are used for placing extraction columns filled with nucleic acid solution; the heating assembly is provided with a first outlet and a first inlet, pipelines are communicated with the first outlet and the first inlet, and the heating assembly conveys a heat medium into the first inlet and conveys the heat medium into the pipelines; the nucleic acid extraction column comprises a heating assembly, a first inlet, a second inlet, a heating groove, a plurality of heat transfer pipes, a heating plate and a first inlet, wherein the first inlet is communicated with the heating groove, the heating groove is communicated with the first inlet, the first inlet is communicated with the heating plate, the heating plate is communicated with the heating groove, one end of each heat transfer pipe, which is opposite to the first inlet, extends into the heating plate in a surrounding mode, the heating groove is arranged in a surrounding mode, and a heat medium which exchanges heat in the heating groove is conveyed into the heating assembly through the first inlet.
Description
Technical Field
The invention relates to the technical field of nucleic acid extraction equipment, in particular to a heating device applied to a nucleic acid extractor and the nucleic acid extractor.
Background
The biological macromolecular compound formed by polymerizing nucleic acid with many nucleotides is one of the most basic substances of life, and the nucleic acid is widely existed in animal and plant cells and microorganisms, and the nucleic acid in the organisms is often combined with protein to form nucleoprotein. In the prior art, most of methods and apparatuses for extracting nucleic acids are disclosed in chinese patent document CN 101886037A. Injecting a sample solution containing nucleic acid into an extraction column positioned above a waste liquid container, injecting compressed air into the extraction column, and applying pressure to the sample solution, so that the sample solution passes through a porous adsorption membrane and is adsorbed on the porous adsorption membrane; then injecting the washing solution into the extraction column, introducing compressed air into the extraction column, and retaining the nucleic acid on the porous adsorption membrane of the extraction column for removing other impurities; then the recovery liquid is injected into the extraction column, compressed air is introduced into the extraction column to apply pressure, so that the porous adsorption membrane is separated from the nucleic acid, and the nucleic acid is recovered by the recovery liquid.
During the whole process of extracting nucleic acid, it is necessary to constantly heat the extraction column so that the solution in the extraction column is heated to a constant temperature and the extraction of nucleic acid is performed at the constant temperature. The heating mechanism in the extracting device comprises a heating plate, a heating sheet and a heat insulation plate, wherein the heating sheet is arranged on the bottom surface of the heating plate, the heat insulation plate is arranged on the top surface of the heating plate, a heating hole is formed in the heating plate, a through hole is correspondingly formed in the heat insulation plate, and the extracting column is installed in the heating hole. The heating plate adopts resistance heating to transfer heat to the heating plate, and then transfers the heat to the extraction column and the solution in the extraction column.
However, this heating method is fast in heating the bottom of the extraction column near the heating plate and slow in heating the top or middle of the extraction column far from the heating plate, and the solution in the extraction column cannot be uniformly heated within the same heating time, which affects the quality of nucleic acid extraction.
Disclosure of Invention
Therefore, the invention aims to solve the technical problem that a heating mechanism in a nucleic acid extractor heats solutions at different heights in an extraction column unevenly, thereby providing a heating device applied to the nucleic acid extractor and the nucleic acid extractor.
A heating device applied to a nucleic acid extractor comprises a heating plate, wherein a plurality of heating grooves are formed in the heating plate, and the heating grooves are used for placing extraction columns filled with nucleic acid solution;
the heating assembly is provided with a first outlet and a first inlet, pipelines are communicated with the first outlet and the first inlet, and the heating assembly conveys a heat medium into the first inlet and conveys the heat medium into the pipelines;
a plurality of heat delivery pipes are communicated with the same opening on the pipeline, the heat delivery pipes are communicated with the heating groove, one ends of the heat delivery pipes, back to the pipeline, extend into the heating plate in a surrounding manner and are arranged around the circumferential edge of the heating groove, the pipeline on the first inlet is communicated with the heating groove, and a heat medium subjected to heat exchange in the heating groove is conveyed into the heating assembly through the pipeline on the first inlet;
and the sealing element is suitable for sealing the notch of the heating groove.
Furthermore, a U-shaped heat conducting pad is arranged in the heating groove, a space is reserved between the heat conducting pad and the inner wall of the heating groove, the direction of a U-shaped opening of the heat conducting pad is consistent with that of a notch of the heating groove, and the extraction column is located in the heat conducting pad and is attached to the heat conducting pad.
Furthermore, the length and the cross section of the heat feeding pipe at the same heating groove are consistent.
Furthermore, a plurality of heating holes are formed in the heating plate, heating rods are arranged in the heating holes, and the heating rods are electrically connected with an external controller.
Further, the sealing member includes the sealing ring, be provided with annular sealed piece on the sealing ring lateral wall, the hot plate is located sealed piece department and is provided with the seal groove, sealed piece is located the seal groove and supports tightly with the seal groove, the standing groove has been seted up on the sealing ring inside wall, heat conduction pad U type mouth one end stretches into in the standing groove and with the laminating of standing groove inner wall.
Furthermore, the outer side walls of the heating plate and the pipeline are both provided with heat insulation films or heat insulation layers, and the heat insulation films or the heat insulation layers cover the outer side walls of the heating plate and the pipeline.
Furthermore, the heating device also comprises a temperature control assembly which is used for detecting the temperature of the heat medium in the heating assembly and controlling the heating assembly to be switched on and off according to the temperature of the heat medium.
Further, the temperature control component comprises at least two temperature sensors, two of the temperature sensors are respectively used for detecting the temperature of the heat medium at the heating component and the temperature in the heating plate, and the temperature sensors are electrically connected with the heating component and the heating rod through a controller.
Further, the height in the heat conducting pad is not lower than the liquid level height of the core acid solution in the extraction column;
and/or the shape of the inner accommodating space of the heating groove and the heat conducting pad is consistent with the shape of the extraction column.
A nucleic acid extractor, characterized by comprising the heating device;
further comprising:
the gas supply device is used for providing required pressure for the nucleic acid liquid in the extraction column;
an extraction column;
the fixing mechanism is provided with a plurality of mounting holes, and the extraction columns are correspondingly inserted into the mounting holes one by one;
and the injection mechanism is used for injecting the washing liquid or the recovery liquid into the extraction column.
The technical scheme of the invention has the following advantages:
1. the heating device applied to the nucleic acid extraction instrument comprises a heating plate, wherein a plurality of heating grooves are formed in the heating plate, and the heating grooves are used for placing extraction columns filled with nucleic acid solution; the heating assembly is provided with a first outlet and a first inlet, pipelines are communicated with the first outlet and the first inlet, and the heating assembly conveys a heat medium into the first inlet and conveys the heat medium into the pipelines; a plurality of heat delivery pipes are communicated with the same opening on the pipeline, the heat delivery pipes are communicated with the heating groove, one ends of the heat delivery pipes, back to the pipeline, extend into the heating plate in a surrounding manner and are arranged around the circumferential edge of the heating groove, the pipeline on the first inlet is communicated with the heating groove, and a heat medium subjected to heat exchange in the heating groove is conveyed into the heating assembly through the pipeline on the first inlet; sealing member, be suitable for and seal up heating groove notch department, when using, heating element carries the pipeline with hot medium in through first export, carry each and send the opening part of heat pipe intercommunication with hot medium through the pipeline, carry many heat pipe departments of sending of same heating groove with the even transport of hot medium through same opening, thereby carry the hot medium in the heating groove along even the transport with the week of heating groove through many heat pipe sending, make hot medium full of whole heating groove, just can carry out the bulk heating to the wall of putting into the extraction post of heating groove, thereby make and be located the nucleic acid solution of not co-altitude in the extraction post just can be by even heating, make each position of nucleic acid solution in the extraction post can both keep certain constant temperature state, thereby improve the quality of nucleic acid extraction.
2. The invention provides a heating device applied to a nucleic acid extraction instrument, wherein a U-shaped heat conducting pad is arranged in a heating groove, a space is reserved between the heat conducting pad and the inner wall of the heating groove, the direction of a U-shaped opening of the heat conducting pad is consistent with that of a notch of the heating groove, an extraction column is positioned in the heat conducting pad and is attached to the heat conducting pad, before the heating device is used, the extraction column is firstly placed in the heat conducting pad in the heating groove, so that the heat conducting pad is tightly abutted against the outer side wall of the extraction column, when in heating, a heating assembly starts to work, so that a heat medium is conveyed into a pipeline through a first outlet and is uniformly conveyed into a plurality of heat conveying pipes through openings corresponding to the heating groove, the heat conveying pipes uniformly convey the heat medium into the heating groove from the periphery of the heating groove, so that the heating groove is filled with the heat medium, and the heat medium in the heating groove heats the whole heat conducting pad, under the effect of heat conduction pad, with the even transmission of heat to the post of drawing that is located the heat conduction pad avoid on, carry out the even heating to drawing the post, make the nucleic acid solution homoenergetic that is located the not co-altitude of drawing the post can be by even heating for draw each position of nucleic acid solution in the post and can both keep certain constant temperature state, thereby improve the quality that nucleic acid drawed.
3. According to the heating device applied to the nucleic acid extraction instrument, the length and the cross section shape of the heat delivery pipes at the same heating groove are consistent, when the heating assembly delivers the heat medium into the pipeline, the heat medium is uniformly delivered into the heat delivery pipes at the opening of the pipeline corresponding to the heating groove, and the time and the amount of the heat medium delivered into the heating groove by the heat delivery pipes are consistent due to the consistent length and the consistent cross section shape of the heat delivery pipes, so that the heat delivery pipes deliver the heat medium into the annular side wall of the heating groove at the same time and in the same amount, the heating groove is filled with the heat medium, and the nucleic acid solutions at different heights in the extraction column can be uniformly heated.
4. According to the heating device applied to the nucleic acid extraction instrument, the heating plate is internally provided with the plurality of heating holes, the heating rods are arranged in the heating holes and are electrically connected with an external controller, and the heating rods are started to heat the heating plate before the extraction column is placed through the arrangement of the heating holes, so that the temperature of the heating plate reaches a constant temperature, the extraction column is placed in the heating groove to heat the extraction column, and the quality of nucleic acid extraction is improved.
5. The heating device applied to the nucleic acid extractor provided by the invention comprises a sealing ring, wherein an annular sealing block is arranged on the outer side wall of the sealing ring, a sealing groove is formed in the position, located at the sealing block, of the heating plate, the sealing block is located in the sealing groove and is abutted against the sealing groove, a placing groove is formed in the inner side wall of the sealing ring, one end of a U-shaped opening of the heat conducting pad extends into the placing groove and is attached to the inner wall of the placing groove, the heating groove and the extraction column are sealed through the arrangement of the sealing ring, and the phenomenon that a heat medium leaks out from a gap between the heating groove and the extraction column in the heating process to influence the heating effect on the extraction column is avoided.
6. According to the heating device applied to the nucleic acid extraction instrument, the outer side walls of the heating plate and the pipeline are respectively provided with the heat insulation film or the heat insulation layer, the heat insulation film or the heat insulation layer covers the outer side walls of the heating plate and the pipeline, and the heat insulation of the heating plate and the pipeline is realized through the arrangement of the heat insulation film or the heat insulation layer, so that the heat in the heating plate and the pipeline is reduced to be radiated from the outer walls of the heating plate and the pipeline, the utilization rate of a heat medium of a heat component is improved, and resources are solved.
7. The heating device applied to the nucleic acid extractor further comprises a temperature control assembly, wherein the temperature control assembly is used for detecting the temperature of a heat medium in the heating assembly and controlling the heating assembly to be turned on and off according to the temperature of the heat medium, the temperature of the heat medium in the heating assembly can be detected through the temperature control assembly, when the temperature of the heat medium in the heating assembly reaches a preset temperature, a heating mode of the heating assembly is turned off, the heating assembly conveys the heat medium into a pipeline, when the temperature of the heat medium in the heating assembly is lower than the preset temperature, the heating mode of the heating assembly is turned on again, the heat-exchanged heat medium entering through the first inlet is heated, the heat medium conveyed by the heating assembly reaches the preset temperature, continuous work of the heating assembly is reduced, and waste of resources is caused.
8. The heating device applied to the nucleic acid extractor further comprises a gas supply device, a gas extraction device and a gas control device, wherein the gas supply device is used for supplying required pressure to the nucleic acid liquid in the extraction column; an extraction column; the fixing mechanism is provided with a plurality of mounting holes, and the extraction columns are correspondingly inserted into the mounting holes one by one; the injection mechanism is used for injecting the washing liquid or the recovery liquid into the extraction column, and the nucleic acid extraction instrument adopts the heating device, so that nucleic acid solutions at different heights in the extraction column can be uniformly heated in the extraction process of nucleic acid, and the required constant temperature state can be maintained, thereby improving the quality of nucleic acid extraction.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic view showing the overall configuration of a heating apparatus of the present invention applied to a nucleic acid isolation apparatus;
fig. 2 is an enlarged view of a portion a in fig. 1.
Description of reference numerals:
1. heating plates; 2. a heating tank; 3. a heating assembly; 31. a first inlet; 32. a first outlet; 33. a pipeline; 34. a heat transfer pipe; 4. a seal member; 41. a seal ring; 42. a sealing groove; 43. a sealing block; 44. a placement groove; 5. a thermally conductive pad; 6. heating the hole; 7. a controller; 8. a temperature sensor; 9. the rod is heated.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In addition, the technical features involved in the different embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
Examples
Referring to fig. 1 and 2, the present invention provides a heating device applied to a nucleic acid extractor, comprising a heating plate 1, a heating assembly 3 and a sealing member 4, wherein the heating plate 1 is provided with a plurality of heating grooves 2, and the heating grooves 2 are used for placing extraction columns containing nucleic acid solutions; the heating assembly 3 is provided with a first outlet 32 and a first inlet 31, the first outlet 32 and the first inlet 31 are communicated with a pipeline 33, and the heating assembly 3 conveys a heat medium into the pipeline 33 through the first inlet 31; a plurality of heat transfer pipes 34 are communicated with the same opening of the pipeline 33, the heat transfer pipes 34 are communicated with the heating groove 2, one ends of the heat transfer pipes 34, which are opposite to the pipeline 33, extend into the heating plate 1 in a surrounding manner and are arranged around the periphery of the heating groove 2, the pipelines 33 on the first inlet 31 are communicated with the heating groove 2, and a heat medium after heat exchange in the heating groove 2 is transferred into the heating assembly 3 through the pipelines 33 on the first inlet 31; the sealing member 4 is adapted to seal the notch of the heating groove 2.
The heating unit 3 is preferably a heating furnace or other structure capable of delivering a heat medium gas.
When the device is used, the heating component 3 conveys the heat medium into the pipeline 33 through the first outlet 32, the heat medium is conveyed to each opening communicated with the heat conveying pipe 34 through the pipeline 33, the heat medium is uniformly conveyed to the plurality of heat conveying pipes 34 of the same heating tank 2 through the same opening, the heat medium is uniformly conveyed into the heating tank 2 from the periphery of the heating tank 2 through the plurality of heat conveying pipes 34, the whole heating tank 2 is filled with the heat medium, the wall surface of the extraction column placed into the heating tank 2 can be integrally heated, the nucleic acid solutions at different heights in the extraction column can be uniformly heated, all positions of the nucleic acid solution in the extraction column can be kept in a certain constant temperature state, and the quality of nucleic acid extraction is improved.
A U-shaped heat conducting pad 5 is arranged in the heating groove 2, a space is reserved between the heat conducting pad 5 and the inner wall of the heating groove 2, the U-shaped opening of the heat conducting pad 5 is consistent with the direction of the notch of the heating groove 2, the extraction column is positioned in the heat conducting pad 5 and is attached to the heat conducting pad 5, before the heat conducting pad is used, the extraction column is firstly placed in the heat conducting pad 5 in the heating groove 2, so that the heat conducting pad 5 is abutted against the outer side wall of the extraction column, when the heat conducting pad is heated, the heating assembly 3 starts to work, so that a heat medium is conveyed into the pipeline 33 through the first outlet 32 and is uniformly conveyed into the plurality of heat conveying pipes 34 through the openings corresponding to the heating groove 2, the heat conveying pipes 34 uniformly convey the heat medium into the heating groove 2 from the periphery of the heating groove 2, so that the heat medium is filled in the heating groove 2, and the heat conducting pad 5 is integrally heated by the heat in the heating groove 2, under the effect of heat conduction pad 5, with the even transmission of heat to the extraction post that is located heat conduction pad 5 avoid on, carry out the even heating to extracting the post, make to be located the nucleic acid solution homoenergetic of extracting the co-altitude in the post by even heating for each position of nucleic acid solution in extracting the post can both keep certain constant temperature state, thereby improves the quality that nucleic acid drawed.
The height in the heat conducting pad 5 is not lower than the liquid level height of the core acid solution in the extraction column; and/or the shape of the internal accommodating space of the heating groove 2 and the heat conducting pad 5 is consistent with the shape of the extraction column.
As an alternative embodiment, the heating tank 2 in the present apparatus may have other longitudinal cross-sectional shapes, as long as it is consistent with the outer shape of the extraction column.
Preferably, the height of the heating tank 2 is 1.2 times, or 1.1 times, or 1.4 times, etc. the height of the nucleic acid solution in the extraction column. The nucleic acid solution in the extraction column can be uniformly heated only by the heating tank 2.
Same length and the cross sectional shape of the heat transfer pipe 34 of heating tank 2 department are all unanimous, when heating element 3 carried the heat medium in pipeline 33, the opening part of pipeline 33 that heating tank 2 corresponds at this moment carries the heat medium in many heat transfer pipes 34 in unison, because the length and the cross sectional shape of heat transfer pipe 34 are all unanimous, the time and the volume that heat transfer pipe 34 carried the heat medium in heating tank 2 are unanimous this moment, thereby guaranteed that heat transfer pipe 34 carries the heat medium in the annular lateral wall of heating tank 2 with the same time of volume, make the heating tank 2 fill with the heat medium, further guaranteed that the nucleic acid solution that is located the interior not co-altitude of extraction post just can be by even heating.
Set up a plurality of heating holes 6 in the hot plate 1, be provided with heating rod 9 in the heating hole 6, heating rod 9 still with 7 electric connection of outside controller, through the setting of heating hole 6 to before putting into and drawing the post, start heating rod 9, heating rod 9 heats hot plate 1, make the temperature of hot plate 1 reach constant temperature, thereby put into in heating bath 2 and draw the post, come to heat drawing the post, improve the quality that the nucleic acid drawed.
Sealing member 4 includes sealing ring 41, be provided with annular sealed piece 43 on the sealing ring 41 lateral wall, hot plate 1 is located sealed piece 43 department and is provided with seal groove 42, sealed piece 43 is located seal groove 42 and supports tightly with seal groove 42, standing groove 44 has been seted up on the sealing ring 41 inside wall, 5U type mouth one end of heat conduction pad stretches into in standing groove 44 and with the laminating of standing groove 44 inner wall, through the setting of sealing ring 41 to with heating groove 2 and draw sealed between the post, avoided in the heating process, the heat medium leaks out from heating groove 2 and the clearance of drawing between the post, influence the heating effect to drawing the post.
All be provided with thermal-insulated membrane or heat preservation on the lateral wall of hot plate 1 and pipeline 33, thermal-insulated membrane or heat preservation cover the lateral wall of hot plate 1 and pipeline 33 through the setting of thermal-insulated membrane or heat preservation to it is thermal-insulated to keep warm to hot plate 1 and pipeline 33, reduces the heat in hot plate 1 and the pipeline 33 and distributes away from hot plate 1 and pipeline 33 outer wall, has improved the utilization ratio of the hot medium that just loves hot subassembly, has solved the resource.
The heating device is characterized by further comprising a temperature control assembly, wherein the temperature control assembly is used for detecting the temperature of the heat medium in the heating assembly 3 and controlling the heating assembly 3 to be turned on and off according to the temperature of the heat medium, the temperature of the heat medium in the heating assembly 3 can be detected through the temperature control assembly, when the temperature of the heat medium in the heating assembly 3 reaches a preset temperature, the heating mode of the heating assembly 3 is turned off at the moment, the heat medium is conveyed into the pipeline 33 by the heating assembly 3, when the temperature of the heat medium in the heating assembly 3 is lower than the preset temperature, the heating mode of the heating assembly 3 is started again at the moment, the heat-exchanged heat medium entering through the first inlet 31 is heated, the heat medium conveyed by the heating assembly 3 reaches the preset temperature, continuous work of the heating assembly 3 is reduced, and waste of resources is caused.
Specifically, the temperature control assembly includes two temperature sensor 8, two temperature sensor 8 is used for detecting respectively heating element 3 department's temperature of hot medium and the temperature in the hot plate 1, temperature sensor 8 through controller 7 with heating element 3 and heating rod 9 electric connection.
As a variation, the temperature control assembly includes three temperature sensors 8, and the three temperature sensors 8 are respectively disposed on the first inlet 31 and the first outlet 32, and the heating plate 1 to respectively detect the temperature of the heat medium output from the heating assembly 3, the temperature of the heat-exchanged heat medium entering from the first inlet 31, and the temperature of the heating plate 1, so that the controller 7 controls the opening or closing of the heating assembly 3 to ensure that the heat medium entering the heating tank 2 is maintained at the same temperature level.
Example 2
A nucleic acid extractor comprises the heating device, a gas supply device, an extraction column, a fixing mechanism and an injection mechanism, wherein the heating device is fixed at the bottom of the fixing mechanism and used for uniformly heating the extraction column; the fixing mechanism is provided with a plurality of mounting holes, and the extraction columns are correspondingly inserted into the mounting holes one by one; the injection mechanism is used for injecting the washing liquid or the recovery liquid into the extraction column, and the nucleic acid extraction instrument adopts the heating device, so that nucleic acid solutions at different heights in the extraction column can be uniformly heated in the extraction process of nucleic acid, and the required constant temperature state can be maintained, thereby improving the quality of nucleic acid extraction.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (10)
1. A heating device applied to a nucleic acid extraction instrument comprises a heating plate (1), and is characterized in that a plurality of heating grooves (2) are formed in the heating plate (1), and the heating grooves (2) are used for placing extraction columns filled with nucleic acid solution;
the heating assembly (3) is provided with a first outlet (32) and a first inlet (31), pipelines (33) are communicated with the first outlet (32) and the first inlet (31), and the heating assembly (3) conveys a heat medium into the first inlet (31) and into the pipelines (33);
a plurality of heat delivery pipes (34) are communicated with the same opening on the pipeline (33), the heat delivery pipes (34) are communicated with the heating groove (2), one ends of the heat delivery pipes (34) back to the pipeline (33) extend into the heating plate (1) in a surrounding mode and are arranged in a surrounding mode around the periphery of the heating groove (2), the pipelines (33) on the first inlet (31) are communicated with the heating groove (2), and heat medium after heat exchange in the heating groove (2) is conveyed into the heating assembly (3) through the pipelines (33) on the first inlet (31);
and the sealing element (4) is suitable for sealing the notch of the heating groove (2).
2. The heating device for use in a nucleic acid isolation apparatus according to claim 1, wherein a U-shaped heat conduction pad (5) is disposed in the heating tank (2), a space is provided between the heat conduction pad (5) and the inner wall of the heating tank (2), the U-shaped opening of the heat conduction pad (5) and the opening of the heating tank (2) are aligned, and the extraction column is disposed in the heat conduction pad (5) and attached to the heat conduction pad (5).
3. The heating device for use in a nucleic acid isolation apparatus according to claim 1, wherein the heat transfer pipe (34) is identical in length and cross-sectional shape to each other in the heating tank (2).
4. The heating device applied to the nucleic acid extractor as claimed in claim 1, wherein a plurality of heating holes (6) are formed in the heating plate (1), a heating rod (9) is disposed in the heating holes (6), and the heating rod (9) is further electrically connected to an external controller (7).
5. The heating device applied to the nucleic acid extractor as claimed in claim 2, wherein the sealing member (4) comprises a sealing ring (41), an annular sealing block (43) is disposed on an outer side wall of the sealing ring (41), a sealing groove (42) is disposed at a position of the heating plate (1) located at the sealing block (43), the sealing block (43) is located in the sealing groove (42) and abuts against the sealing groove (42), a placing groove (44) is disposed on an inner side wall of the sealing ring (41), and one end of a U-shaped opening of the heat conducting pad (5) extends into the placing groove (44) and is attached to an inner wall of the placing groove (44).
6. The heating device applied to the nucleic acid extractor as claimed in claim 1, wherein the outer side walls of the heating plate (1) and the pipe (33) are provided with a heat insulation film or layer, and the heat insulation film or layer covers the outer side walls of the heating plate (1) and the pipe (33).
7. The heating device applied to the nucleic acid extractor as claimed in claim 1, further comprising a temperature control unit for detecting the temperature of the heating medium in the heating unit (3) and controlling the heating unit (3) to be turned on or off according to the temperature of the heating medium.
8. The heating device applied to the nucleic acid extractor as claimed in claim 7, wherein the temperature control assembly comprises at least two temperature sensors (8) for detecting the temperature of the heat medium at the heating assembly (3) and the temperature inside the heating plate (1), respectively, and the temperature sensors (8) are electrically connected with the heating assembly (3) and the heating rod (9) through a controller (7).
9. The heating device for use in the nucleic acid extractor according to claim 5, wherein the height of the heat conductive pad (5) is not lower than the liquid level of the nucleic acid solution in the extraction column;
and/or the shape of the internal accommodating space of the heating groove (2) and the heat conducting pad (5) is consistent with the shape of the extraction column.
10. A nucleic acid extracting apparatus comprising the heating device according to any one of claims 1 to 9;
further comprising:
the gas supply device is used for providing required pressure for the nucleic acid liquid in the extraction column;
an extraction column;
the fixing mechanism is provided with a plurality of mounting holes, and the extraction columns are correspondingly inserted into the mounting holes one by one;
and the injection mechanism is used for injecting the washing liquid or the recovery liquid into the extraction column.
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| WO2011004653A1 (en) * | 2009-07-09 | 2011-01-13 | 凸版印刷株式会社 | Nucleic acid extraction kit, nucleic acid extraction method, and nucleic acid extraction apparatus |
| CN106467891A (en) * | 2016-09-22 | 2017-03-01 | 苏州百源基因技术有限公司 | A kind of gas supply device being applied to instrument for extracting nucleic acid and instrument for extracting nucleic acid |
| CN108102875A (en) * | 2016-11-24 | 2018-06-01 | 苏州百源基因技术有限公司 | A kind of heating structure and instrument for extracting nucleic acid applied in nucleic acid-extracting apparatus |
| CN210856144U (en) * | 2019-10-15 | 2020-06-26 | 上海畅亨工贸有限公司 | Nucleic acid automatic extraction instrument with uniform heating |
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