CN220393752U - EP tube heating device for assisting nucleic acid extraction - Google Patents
EP tube heating device for assisting nucleic acid extraction Download PDFInfo
- Publication number
- CN220393752U CN220393752U CN202321904042.9U CN202321904042U CN220393752U CN 220393752 U CN220393752 U CN 220393752U CN 202321904042 U CN202321904042 U CN 202321904042U CN 220393752 U CN220393752 U CN 220393752U
- Authority
- CN
- China
- Prior art keywords
- heating
- nucleic acid
- acid extraction
- outer cylinder
- pipe
- Prior art date
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- 238000010438 heat treatment Methods 0.000 title claims abstract description 76
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 20
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 20
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 20
- 238000000605 extraction Methods 0.000 title claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims abstract description 13
- 235000017491 Bambusa tulda Nutrition 0.000 claims abstract description 13
- 241001330002 Bambuseae Species 0.000 claims abstract description 13
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims abstract description 13
- 239000011425 bamboo Substances 0.000 claims abstract description 13
- 230000006835 compression Effects 0.000 claims description 6
- 238000007906 compression Methods 0.000 claims description 6
- 238000012856 packing Methods 0.000 abstract description 2
- 238000003780 insertion Methods 0.000 description 8
- 230000037431 insertion Effects 0.000 description 8
- 239000002184 metal Substances 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000012864 cross contamination Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000002452 interceptive effect Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002457 bidirectional effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005338 heat storage Methods 0.000 description 1
- -1 human genome Chemical class 0.000 description 1
- 239000012774 insulation material Substances 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000007779 soft material Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
Abstract
The application provides an EP pipe heating device for assisting nucleic acid extraction, including the urceolus, be equipped with in the urceolus and hold the chamber, hold intracavity packing heating liquid, the urceolus upper end is equipped with the heating pot head, and the heating pot head is equipped with a plurality of down and stretches a section of thick bamboo, stretches down and stretches into and hold the intracavity, stretches down and is equipped with the EP pipe jack in the section of thick bamboo, and the EP pipe jack is the blind hole, and the EP pipe jack is used for inserting the EP pipe, has solved the problem of EP pipe constant temperature heating when nucleic acid extraction.
Description
Technical Field
The utility model relates to the field of biological detection, in particular to an EP tube heating device for assisting nucleic acid extraction.
Background
The EP tube is also called a centrifuge tube, is a small plastic tube used for extracting and centrifuging trace sample nucleic acid such as human genome, animals, plants, pathogens and the like, and is used as a carrier for containing a nucleic acid extraction reagent system in the process of extracting sample nucleic acid, and is used for heating and preserving heat in the process of extracting nucleic acid samples in the EP tube, so that the reaction of surfactants, proteases and the like in the extraction reagent is accelerated at a proper temperature, and the extraction efficiency and the nucleic acid yield are improved.
The existing EP pipe heating device adopts a heating disc to heat the lower end of a metal sleeve block entity in a form of a porous belt, and is unfavorable for a test because the specific heat capacity of metal is smaller and larger temperature fluctuation can occur due to external influence when the EP pipe is insulated.
In another mode, a hollow bracket with multiple holes is adopted, a group of EP pipes are inserted on the hollow bracket, the hollow bracket is immersed in a water bath device, the mode also has certain defects, if the EP pipes are not tightly sealed, heating liquid and a sample can be subjected to bidirectional cross contamination, and workers can be contacted with the polluted heating liquid when lifting the hollow bracket.
Disclosure of Invention
The utility model provides an EP tube heating device for assisting nucleic acid extraction, which solves the problem of constant-temperature heating of an EP tube during nucleic acid extraction.
In order to solve the technical problems, the utility model adopts the following technical scheme: the utility model provides an EP pipe heating device for assisting nucleic acid extraction, includes the urceolus, is equipped with in the urceolus and holds the chamber, holds intracavity packing heating liquid, and the urceolus upper end is equipped with the heating pot head, and the heating pot head is equipped with a plurality of down and stretches a section of thick bamboo, stretches down a section of thick bamboo and stretches into and hold the intracavity, stretches down and is equipped with the EP pipe jack in the section of thick bamboo, and the EP pipe jack is the blind hole, and the EP pipe jack is used for inserting the EP pipe.
In the preferred scheme, the heating end sleeve is sleeved with the outer cylinder, the heating end sleeve is provided with a concave cavity, the bottom end of the concave cavity is provided with an exhaust hole, the exhaust hole is communicated with the containing cavity, and a detachable exhaust hole plug is arranged in the exhaust hole.
In the preferred scheme, the outer wall of the outer cylinder is provided with a threaded part near the upper end, and one end of the compression ring is propped against the heating end sleeve, and the other end of the compression ring is in threaded connection with the outer cylinder.
In the preferred scheme, the urceolus lateral wall is equipped with the indent section of thick bamboo that is equipped with the thermocouple jack to holding intracavity protrusion, is equipped with the thermocouple in the indent section of thick bamboo.
In the preferred scheme, the heating device is also provided with an outer shell, a heating plate is arranged in the outer shell, the outer cylinder is arranged on the heating plate, and the upper end of the outer shell is provided with an openable upper cover.
In the preferred scheme, a plurality of heat conducting fins which are annularly distributed are arranged at the bottom end of the upper containing cavity of the outer cylinder.
The beneficial effects of the utility model are as follows: the water bath heating and the metal contact heating are combined, the high specific heat capacity of the internal heating liquid is used for heat storage, the metal heating end with the jack is sleeved outside for conducting heat to the EP pipe, so that the temperature stirring of the heat preservation type is reduced, the flow of the heating liquid is limited, and the cross contamination is avoided; the heat of the heating liquid is fully absorbed by adopting the plurality of thin-wall concave cylinders which extend downwards to the heating liquid, so that the contact area is large, and the heat conduction efficiency is improved.
Drawings
The utility model is further described below with reference to the drawings and examples.
Fig. 1 is a schematic diagram of an extended implementation of the present utility model.
Fig. 2 is a schematic diagram of the present utility model.
Fig. 3 is an internal structural view of the present utility model.
Fig. 4 is an exploded view of the present utility model.
Fig. 5 is a structural view of the outer cylinder of the present utility model.
In the figure: an outer cylinder 1; a cavity 101; a positioning groove 102; a heat conductive sheet 103; a concave cylinder 104; thermocouple insertion holes 105; heating the end sleeve 2; a downward extending cylinder 201; EP tube insertion holes 202; a cavity 203; an exhaust hole 204; vent plug 205; a clamp ring 3; a thermocouple 4; a gasket 5; a seal ring 6; an outer case 7; a heating plate 8; an upper cover 9; an interactive panel 10.
Detailed Description
As shown in fig. 1-5, an EP tube heating device for assisting nucleic acid extraction comprises an outer tube 1, wherein a cavity 101 is arranged in the outer tube 1, heating liquid is filled in the cavity 101, a heating end sleeve 2 is arranged at the upper end of the outer tube 1, a plurality of downward extending tubes 201 are arranged on the heating end sleeve 2, the downward extending tubes 201 extend into the cavity 101, EP tube insertion holes 202 are formed in the downward extending tubes 201, the EP tube insertion holes 202 are blind holes, and the EP tube insertion holes 202 are used for inserting EP tubes.
The heating end sleeve 2 is preferably made of a metal material, such as copper, and has good heat conduction performance, and the heating liquid in the cavity 101 is water, oil or other liquid medium with larger specific heat capacity, so that obvious temperature fluctuation is prevented when the liquid in the EP pipe is heated.
The down tube 201 is a thin-walled tube, the lower end is sealed so that liquid does not dip into the EP tube insertion hole 202, and because the heat conductivity of the down tube 201 is high,
the EP tube insertion holes 202 may be arranged in a variety of ways, and the EP tube insertion holes 202 may have a variety of diameters to match EP tubes of different diameters.
The bottom end of the outer cylinder 1 is made of heat conducting materials.
The side wall of the outer cylinder 1 can be wrapped by heat insulation materials, so that the heat loss of the heating liquid is reduced.
In the preferred scheme, the heating pot head 2 is sleeved with the outer cylinder 1, the heating pot head 2 is provided with a cavity 203, the bottom end of the cavity 203 is provided with an exhaust hole 204, the exhaust hole 204 is communicated with the accommodating cavity 101, and a detachable exhaust hole plug 205 is arranged in the exhaust hole 204.
The locating groove 102 is arranged at the position, close to the upper port, of the accommodating cavity 101, the outer edge of the heating end sleeve 2 is sleeved into the locating groove 102, the lower end face of the heating end sleeve 2 abuts against the bottom end of the locating groove 102, the sealing ring 6 is arranged at the outer edge of the heating end sleeve 2, and the sealing gasket 5 is arranged at the bottom end of the locating groove 102, so that the accommodating cavity 101 is good in tightness, and the heating end sleeve 2 is required to be exhausted outwards from the exhaust hole 204 to keep internal and external pressure balance when sleeved into the outer cylinder 1.
The vent hole plug 205 is made of soft materials such as rubber or silica gel, and the vent hole plug 205 is plugged to prevent ash falling.
In the preferred scheme, the outer wall of the outer cylinder 1 is provided with a threaded part near the upper end, and one end of a compression ring 3 is propped against the heating end sleeve 2, and the other end of the compression ring 3 is in threaded connection with the outer cylinder 1.
The heating end sleeve 2 is annular, the section is L-shaped, the L-shaped folded edge can press the outer edge of the heating end sleeve 2, and the inner wall of the heating end sleeve 2 is provided with threads and screwed with the threads of the outer cylinder 1.
In the preferred scheme, the lateral wall of urceolus 1 is equipped with concave section of thick bamboo 104 that is protruding to holding chamber 101, is equipped with thermocouple jack 105 in the concave section of thick bamboo 104, is equipped with thermocouple 4 in the thermocouple jack 105.
In the preferred scheme, the heating device is also provided with an outer shell 7, a heating plate 8 is arranged in the outer shell 7, the outer cylinder 1 is arranged on the heating plate 8, and an openable upper cover 9 is arranged at the upper end of the outer shell 7.
The upper cover 9 may be transparent and one end is hinged to the outer case 7.
The indent section of thick bamboo 104 is thin wall form and thermal conductivity is high, and thermocouple jack 105 is the blind hole, and thermocouple 4 lower extreme is the detection end, in pressing close to holding chamber 101 more, detects the heating liquid temperature in real time.
The thermocouple 4 and the heating plate 8 are electrically connected with an external control system machine power supply system.
The lateral surface of the outer cylinder 1 can be provided with an interactive panel 10.
In a preferred scheme, a plurality of heat conducting fins 103 which are annularly distributed are arranged at the bottom end of the accommodating cavity 101 on the outer barrel 1.
The heat conducting fin 103 can increase the contact area between the outer cylinder 1 and the heating liquid, and improve the heating efficiency of the heating plate 8 on the heating liquid in the cavity 101.
If the thermocouple 4 fails, the problem of the liquid is close to the boiling point when the heating plate 8 heats the outer cylinder 1, the liquid at the bottom end of the cavity 101 will be partially boiled to generate bubbles and increase the air pressure in the cavity 101, at this time, the vent hole plug 205 is extruded and pushed out to the north, so that the vent hole 204 is opened, and the internal pressure is discharged, at this time, part of the liquid overflows, so that the cavity 203 is arranged to prevent the liquid from flowing onto the test bed.
The above embodiments are only preferred embodiments of the present utility model, and should not be construed as limiting the present utility model, and the scope of the present utility model should be defined by the claims, including the equivalents of the technical features in the claims. I.e., equivalent replacement modifications within the scope of this utility model are also within the scope of the utility model.
Claims (6)
1. An EP tube heating device for assisting nucleic acid extraction, characterized by: including urceolus (1), be equipped with in urceolus (1) and hold chamber (101), hold chamber (101) intussuseption and fill heating liquid, urceolus (1) upper end is equipped with heating pot head (2), and heating pot head (2) are equipped with a plurality of down and stretch section of thick bamboo (201), and down stretch section of thick bamboo (201) stretch into hold chamber (101) in, be equipped with EP pipe jack (202) in stretching down section of thick bamboo (201), EP pipe jack (202) are the blind hole, and EP pipe jack (202) are used for inserting the EP pipe.
2. The EP tube heating apparatus for assisting nucleic acid extraction according to claim 1, characterized in that: the heating end sleeve (2) is sleeved with the outer cylinder (1), the heating end sleeve (2) is provided with a concave cavity (203), the bottom end of the concave cavity (203) is provided with an exhaust hole (204), the exhaust hole (204) is communicated with the accommodating cavity (101), and a detachable exhaust hole plug (205) is arranged in the exhaust hole (204).
3. The EP tube heating apparatus for assisting nucleic acid extraction according to claim 1, characterized in that: the outer wall of the outer cylinder (1) is provided with a thread part near the upper end, one end of the compression ring (3) is propped against the heating end sleeve (2), and the other end of the compression ring (3) is in threaded connection with the outer cylinder (1).
4. The EP tube heating apparatus for assisting nucleic acid extraction according to claim 1, characterized in that: the side wall of the outer cylinder (1) is provided with a concave cylinder (104) protruding into the accommodating cavity (101), a thermocouple jack (105) is arranged in the concave cylinder (104), and a thermocouple (4) is arranged in the thermocouple jack (105).
5. The EP tube heating apparatus for assisting nucleic acid extraction according to claim 1, characterized in that: the heating device is characterized by further comprising an outer shell (7), wherein a heating plate (8) is arranged in the outer shell (7), the outer cylinder (1) is arranged on the heating plate (8), and an openable upper cover (9) is arranged at the upper end of the outer shell (7).
6. The EP tube heating apparatus for assisting nucleic acid extraction according to claim 1, characterized in that: the bottom end of the upper containing cavity (101) of the outer cylinder (1) is provided with a plurality of heat conducting fins (103) which are annularly distributed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321904042.9U CN220393752U (en) | 2023-07-19 | 2023-07-19 | EP tube heating device for assisting nucleic acid extraction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321904042.9U CN220393752U (en) | 2023-07-19 | 2023-07-19 | EP tube heating device for assisting nucleic acid extraction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220393752U true CN220393752U (en) | 2024-01-26 |
Family
ID=89611095
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321904042.9U Active CN220393752U (en) | 2023-07-19 | 2023-07-19 | EP tube heating device for assisting nucleic acid extraction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220393752U (en) |
-
2023
- 2023-07-19 CN CN202321904042.9U patent/CN220393752U/en active Active
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |