CN114308987A - Method for in-situ passivation of heavy metals in solid waste generated in mining - Google Patents
Method for in-situ passivation of heavy metals in solid waste generated in mining Download PDFInfo
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Abstract
The invention discloses a method for in-situ passivation of heavy metals in solid waste generated in mining, and belongs to the technical field of heavy metal remediation in environmental engineering. The passivation effect on heavy metals is improved by adding compost sludge of an urban sewage treatment plant to stimulate microorganisms. Under the stimulation effect of the compost sludge on microorganisms, the microorganisms and the compost sludge jointly convert heavy metals from an active form to a stable form, so that the passivation effect on the heavy metals such as copper (Cu), cadmium (Cd), lead (Pb) and the like is achieved, and the leaching concentration of the heavy metals from pollutants and the threat to water and the surrounding ecological environment are reduced.
Description
Technical Field
The invention relates to the technical field of heavy metal remediation in environmental engineering, in particular to a method for in-situ passivation of heavy metals in solid waste generated in mining.
Background
A large amount of solid waste is generated in the mining and smelting process of non-ferrous metals, and mainly comprises slag generated in mining or mineral dressing, smelted tailings and pollution discharge channel sediment containing a large amount of heavy metals accumulated in the day after the smelting wastewater is discharged. Wherein the slay has taken up considerable proportion in the mining area is useless admittedly, for the harm of reduction slay to the environment, often can backfill the mine opening with discarded slay now, but the slay of piling up in the past is very easily got into groundwater or is spread to the surrounding environment after the weathering drenches, threatens ecological environment and resident health. The sediment of the sewage draining channel has high heavy metal content, and the components contained in the medium are more complicated due to the long-term receiving of the smelting wastewater. The development of a feasible heavy metal soil remediation treatment method is very important.
In view of this, the invention is particularly proposed.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides a method for in-situ passivation of heavy metals in solid waste generated in mining.
The invention is realized by the following steps:
the invention provides a method for in-situ passivation of heavy metals in solid waste generated by mining, which is characterized in that enterococcus LZU-1 bacterial liquid is combined with compost sludge to passivate the heavy metals in the solid waste generated by mining;
wherein the heavy metal is selected from at least one of Cu, Cd and Pb;
the preservation number of the enterococcus LZU-1 is CGMCC 22622.
The invention has the following beneficial effects:
the invention provides a method for in-situ passivation of heavy metals in solid waste generated by mining, which is characterized in that enterococcus LZU-1 (with the collection number of CGMCC 22622) with a urease production function is used for passivating the heavy metals in the solid waste generated by mining, and the passivation effect of the heavy metals is improved by adding compost sludge stimulation microorganisms of an urban sewage treatment plant. Under the stimulation effect of the compost sludge on microorganisms, the microorganisms and the compost sludge jointly convert heavy metals from an active form to a stable form, so that the passivation effect on the heavy metals such as copper (Cu), cadmium (Cd), lead (Pb) and the like is achieved, and the leaching concentration of the heavy metals from pollutants and the threat to water and the surrounding ecological environment are reduced.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 shows the leaching concentrations of heavy metals by TCLP method before and after slag passivation;
FIG. 2 shows the leaching concentrations of heavy metals by the TCLP method before and after passivation of the deposit;
FIG. 3 shows the change of heavy metal morphology before and after slag passivation;
FIG. 4 shows the change of heavy metal morphology before and after passivation of the deposit.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
In order to solve the problem of heavy metal pollution of solid wastes (mine area slag and sewage drainage channel sediments) generated in mining, the invention aims to provide a restoration technology for passivating heavy metals in mine area solid wastes by utilizing compost sludge to stimulate urease-producing microorganisms.
The mechanism for passivating the heavy metal by utilizing the urease production function of the microorganism is mainly to utilize the urease production function to induce carbonate precipitation so that carbonate ions are directly combined with the heavy metal ions or are coprecipitated with the heavy metal ions in the process of forming calcium carbonate crystals; in addition, the combination and adsorption of the biomass generated by the microorganisms and the heavy metals can also effectively passivate the heavy metals. In order to further improve the inactivation effect of the microorganisms, additives are often needed to assist the microorganisms in order to make the inactivation product more stable. Compost sludge is a product formed by the decomposition of organic matter by the anaerobic and aerobic action of microorganisms. The addition of compost sludge to the slag or sediment can effectively improve the porosity of pollutant media and increase the oxygen flux. In addition, the compost sludge is used as a microbial fermentation product, the environment is more suitable for the survival of microorganisms, the larger specific surface area of the compost sludge provides more sufficient attachment points for the microorganisms, and a large amount of organic acids and other organic micromolecules are generated after the compost sludge is fermented to provide a nutrient source for the microorganisms. The addition of the material with various advantages to the survival of the microorganisms can be used as a new technology for remedying the heavy metal pollution of the solid waste in the mining area by the microorganisms.
The invention is realized by the following steps: urea is decomposed by utilizing the characteristics of urease-producing bacteria, and carbonate ions generated after the urea is decomposed are combined with added calcium ions to form carbonate precipitates. The heavy metals are either encapsulated in the precipitated crystals as the carbonate precipitate forms, or are passivated by direct binding with carbonate ions. Compost sludge is added into pollutants, the porosity of a medium is improved by using the compost sludge, the oxygen content is increased, bacteria can proliferate by using a large specific surface area and nutrient substances of the compost sludge, and the remediation effect is further improved. After passivation was complete, the effect of heavy metal passivation was evaluated using the TCLP method.
The embodiment of the invention provides a method for in-situ passivation of heavy metals in solid waste generated by mining, which is characterized in that enterococcus LZU-1 bacterial liquid is combined with compost sludge to passivate the heavy metals in the solid waste generated by mining;
wherein the heavy metal is selected from at least one of Cu, Cd and Pb;
the preservation number of the enterococcus LZU-1 is CGMCC 22622.
In an alternative embodiment, the method comprises the following steps: and (3) applying the enterococcus LZU-1 bacterial liquid and the cementing liquid to a mixture of solid waste and compost sludge generated by mining, and culturing at room temperature to passivate heavy metals in the solid waste generated by mining.
In an alternative embodiment, the preparation process of the enterococcus LZU-1 bacterial liquid comprises the following steps: inoculating activated enterococcus LZU-1 in nutrient broth culture medium, performing shaking culture at room temperature, and collecting the bacteria after enrichment cultureDiluting the solution with water to OD6000.8 to 1.2;
preferably, the purified enterococcus LZU-1 is cultured in a shaker at 25-35 ℃ and 100-600Is 0.8-1.2.
In an alternative embodiment, the nutrient broth medium consists of: 4-6g/L of sodium chloride, 2-4g/L of beef extract and 8-12g/L of peptone.
In an alternative embodiment, the composition of the cementing fluid is: 0.01-0.1mol/L of calcium chloride, 0.2-0.7mol/L of urea, 4-6g/L of sodium chloride, 2-4g/L of beef extract and 8-12g/L of peptone.
In an alternative embodiment, the mixture of solid waste and compost sludge produced per gram of mine mining corresponds to a volume of 0.1-1mL of enterococcus LZU-1 bacterial fluid and to a volume of 0.1-1mL of cementitious fluid.
In an alternative embodiment, the composted sludge is a municipal sewage treatment plant sludge that has been composted. Specifically, the compost sludge used in the embodiment of the present invention is obtained by dehydrating sludge from a municipal sewage treatment plant, mixing the dehydrated sludge with straw and microorganisms (such as bacteria), and performing aerobic composting, and preferably, the compost sludge is pulverized into particles having a particle size of less than 2mm before being used for remediation.
In an alternative embodiment, the solid waste from mining is any one of non-ferrous slag and sewer sediment.
In an alternative embodiment, the enterococcus LZU-1 bacteria solution and the cementing solution are poured into the sample and mixed and cultured for 8-12 days.
In an alternative embodiment, the method further comprises: pouring the enterococcus LZU-1 bacterial liquid and the cementing liquid into the sample which is passivated for 8-12 days, and continuing to perform passivating culture for 8-12 days.
The embodiment of the invention provides a scheme for improving the capability of urease-producing bacteria for passivating heavy metals by using compost sludge. Firstly, compost sludge is a product obtained by microbial fermentation, the compost sludge has an excellent environment required by microbial growth, small molecular organic matters in the compost sludge provide richer nutrient substances for microbes, and fluffy particles provide a stable loading position for the microbes, so that the reproduction of the microbes is facilitated. And secondly, after the compost sludge is added into the slag or sediment, the porosity of the original compact slag or sediment medium is greatly increased, the oxygen flux of the medium is effectively improved, an oxygen source is provided for microorganisms deep in the medium, the survival time of the microorganisms in the medium is prolonged, and the calcium carbonate is favorably generated by the microorganisms under the aerobic condition. The heavy metals are solidified under the adsorption action of the re-compost sludge, the free heavy metals such as Cu, Cd, Pb and the like in the slag or the sediment are fixed under the coordination of various actions, the leaching concentration of the heavy metals is reduced, and the ecological risk of the re-compost sludge to surrounding soil, earth surface and underground water is greatly reduced. In conclusion, the enterococcus LZU-1 and the compost sludge are used for solidifying heavy metals in slag and sediments in mining areas, and have great significance for environmental protection and treatment.
The features and properties of the present invention are described in further detail below with reference to examples.
The enterococcus LZU-1 used in the invention is obtained by separating, purifying and identifying from an arid region environment through a conventional method, and the specific separation and purification method is the prior art, and can refer to the separation method of urease-producing bacteria mentioned in "effect and mechanism for mineralizing and repairing Cd and Pb polluted soil by urease-producing bacteria" of Chuihong et al. The obtained 16S rDNA gene of the urease producing bacteria is subjected to BLAST analysis to know that the gene is Enterococcus and has close relationship with Enterococcus sp.123py (NCBI: txid1095537), and the similarity of the 16S rDNA gene of the Enterococcus sp.123py reaches 99.66 percent. Therefore, the strain is named as Enterococcus sp.LZU-1, the gene sequence of the strain is submitted to GenBank to obtain the accession number of MZ021475, meanwhile, the strain is preserved in the China general microbiological culture Collection center, the preservation number of the strain is CGMCC 22622, and the preservation date of the strain is 2021, 5 and 31 days.
The embodiment of the invention provides a method for passivating nonferrous metal slag and heavy metals in sewage canal sediments by combining enterococcus and compost sludge, which comprises the following specific steps:
the first step is as follows: inoculating the purified enterococcus LZU-1 into a nutrient broth culture medium, culturing for 12-30h at 25-35 ℃ in a shaking table with the speed of 100 plus 150rpm, activating, inoculating the activated enterococcus LZU-1 into a conical flask containing 300mL of the nutrient broth culture medium according to the volume ratio of 0.1-0.4%, and performing enrichment culture for 36-60h at 25-35 ℃ in a shaking table with the speed of 150 plus 180 rpm. Diluting the enriched culture bacterial liquid to OD with ultrapure water600Is 0.8-1.2.
Preferably, the nutrient broth medium consists of: 4-6g/L of sodium chloride, 2-4g/L of beef extract and 8-12g/L of peptone.
The second step is that: weighing a certain mass of ore region slag and sewage channel sediment, adding 10% of compost sludge into the ore region slag and sewage channel sediment, and uniformly stirring and mixing the mixture for later use; preferably, the prepared cementing liquid is composed of 0.01-0.1mol/L of calcium chloride, 0.2-0.7mol/L of urea, 4-6g/L of sodium chloride, 2-4g/L of beef extract and 8-12g/L of peptone for later use.
The third step: taking OD600The bacterial liquid and the cementing liquid of 0.8 to 1.2 are respectively applied to the slag of a mining area, the sediment of a sewage drainage channel, the slag mixture containing 10 percent of compost sludge and the sediment mixture containing 10 percent of compost sludge, and are passivated in a glass culture dish for 8 to 12 days at room temperature.
Preferably, each gram of mine area slag, sewage drainage channel sediment, slag mixture and sediment mixture has a corresponding bacterial liquid volume of 0.1-1mL and a corresponding cementing liquid volume of 0.1-1 mL.
The fourth step: and repeating the third step operation on the sample after passivating for 8-12 days in the third step, and continuing to passivate for 8-12 days.
In the embodiment of the invention, under the condition of singly using microorganisms without using compost sludge, the concentrations of Cu, Cd and Pb in the slag leaching liquor of the passivated mining area are respectively reduced by 8.00%, 2.69% and 9.33%; the concentrations of Cd and Pb in the sediment leaching liquor of the sewage draining channel are respectively reduced by 9.84 percent and 49.83 percent.
According to the embodiment of the invention, microorganisms are added into a mixture formed by compost sludge and pollutants, and the concentrations of Cu, Cd and Pb in the slag leaching liquor of the passivated mining area are respectively reduced by 72.17%, 28.34% and 74.76%; the concentrations of Cd and Pb in the sediment leaching liquor of the sewage draining channel are respectively reduced by 10.59 percent and 74.29 percent.
In the embodiment of the invention, the restoration object is at least one of Cu, Cd and Pb in mine area slag or mine area sewage drainage channel sediment.
The mine slag repaired in the embodiment is taken from waste rock heaps of northeast mine areas of Baiyin, Gansu province, the pH value is 7.79, and the concentrations of Cu, Cd and Pb respectively reach 1342.8mg/kg, 27.53mg/kg and 2990.8 mg/kg; the deposit is taken from the upstream of the drainage channel of the mining area, the pH value is 8.33, and the concentrations of Cd and Pb respectively reach 156.3mg/kg and 725.6 mg/kg.
In order to more clearly describe the technical scheme of the invention, the following embodiments are further described:
passivation of heavy metals in pollutants by microbial technology:
embodiment preparation content
Preparing nutrient broth culture medium containing sodium chloride 5g/L, beef extract 3g/L, and peptone 10g/L, sterilizing at 121 deg.C for 20min, cooling, inoculating purified enterococcus LZU-1 into sterilized nutrient broth culture medium, culturing in a shaker at 28 deg.C and 130rpm for 12-30h, inoculating activated urease-producing bacteria into a medium containing 300mL sterilized nutrient broth culture medium at a volume ratio of 0.2%, enriching and culturing in a shaker at 28 deg.C and 180rpm for 48h, and determining OD of bacteria solution600Value, and diluted to OD with ultrapure water600Is 0.8-1.2 for standby.
Preparing a cementing solution of 0.05mol/L calcium chloride, 0.5mol/L urea, 5g/L sodium chloride, 3g/L beef extract and 10g/L peptone for later use.
The compost sludge, the slag and the sediment are air-dried and ground to pass through a sieve with the aperture of 2mm, the compost sludge is added into the slag and the sediment of a sewage drainage channel according to the mass ratio of 10% to obtain a slag mixture containing 10% of compost sludge and a sediment mixture containing 10% of compost sludge, and 50g of the slag, the slag and 10% of compost sludge mixture, the sediment and the 10% of compost sludge mixture are respectively weighed and placed in a culture dish for later use.
Passivation of heavy metals in pollutants
Example 1
OD obtained from the preliminary content600Respectively and uniformly pouring 10mL of bacterial liquid and 15mL of cementing liquid which are 0.8-1.2 into a glass culture dish of a mixture of 50g of slag, 50g of slag and 10% of compost sludge for standby in the prepared content, and performing passivation culture for 10 days at room temperature; after 10 days, likewise, OD was again added600Respectively and uniformly pouring 10mL of bacterial liquid and 15mL of cementing liquid which are 0.8-1.2 into the samples which are passivated for 10 days, and continuously performing the passivation culture for 10 days. The entire repair experiment was 20 days.
Example 2
OD obtained from the preliminary content600Respectively and uniformly pouring 10mL of bacterial liquid and 15mL of cementing liquid which are 0.8-1.2 into a glass culture dish of a mixture of 50g of sediment, 50g of sediment and 10% of compost sludge for standby in the preparation content, and performing passivation culture for 10 days at room temperature; after 10 days, likewise, OD was again added600Respectively and uniformly pouring 10mL of bacterial liquid and 15mL of cementing liquid which are 0.8-1.2 into the samples which are passivated for 10 days, and continuously performing the passivation culture for 10 days. The entire repair experiment was 20 days.
Assessment of passivation effect of heavy metals in pollutants
After the passivation experiment is finished, the samples obtained in the example 1 and the example 2 are air-dried, the TCLP standard method is used for leaching the samples, and heavy metals with different forms in the samples are extracted by a Tessier five-step extraction method. And (3) measuring the concentration of the heavy metal in the obtained leaching solution or extracting solution by using an atomic absorption spectrometer (American thermoelectric ICE-3500).
As can be seen from FIG. 1, in example 1, after the enterococcus LZU-1 bacterial liquid was added, the leachable concentrations of Cu, Cd and Pb in the slag were respectively reduced from 9.92mg/L, 0.51mg/L and 3.56mg/L to 9.13mg/L, 0.50mg/L and 3.23mg/L, which were respectively reduced by 8.00%, 2.69% and 9.33%; after bacterial liquid is added into slag containing 10% of compost sludge, the heavy metal passivation efficiency is remarkably improved, the leachable concentrations of Cu, Cd and Pb in the slag are respectively reduced to 2.76mg/L, 0.37mg/L and 0.90mg/L, and the passivation efficiencies respectively reach 72.17%, 28.34% and 74.76%.
As can be seen from FIG. 2, for example 2, after the enterococcus LZU-1 bacterial liquid is added, the leaching concentrations of Cd and Pb in the sediment are respectively reduced from 2.42mg/L and 0.30mg/L to 2.18mg/L and 0.15mg/L, which are respectively reduced by 9.84 percent and 49.83 percent; after bacterial liquid is added into the sediment containing 10% of compost sludge, the leachable concentration of Cd and Pb is reduced to 2.16mg/L and 0.08mg/L, and the passivation efficiency respectively reaches 10.59% and 74.29%.
As can be seen from FIGS. 3 and 4, under the conditions of remediation using only microorganisms, the exchangeable heavy metal content was decreased in both examples 1 and 2; under the assistance of the compost sludge, the content of exchangeable heavy metals is further reduced, and the increased specific gravity of a more stable residue state and an organic matter combined state is larger.
Comparative example 1
Similar to the procedure of examples 1 and 2, except that: when the microorganism and the compost sludge are jointly repaired, the consumption of the mixed compost sludge in the nonferrous metal slag and the sewage canal sediment is 5 percent. After restoration, the leachable concentrations of Cu, Cd and Pb in the slag are respectively reduced from 9.92mg/L, 0.51mg/L and 3.56mg/L to 4.82mg/L, 0.42mg/L and 1.87mg/L, and the passivation efficiencies are respectively 51.41%, 17.65% and 47.47%; the leachable concentrations of Cd and Pb in the sediment of the sewage draining channel are respectively reduced from 2.42mg/L and 0.30mg/L to 2.21mg/L and 0.05mg/L, and the passivation efficiencies are respectively 8.68 percent and 83.33 percent.
Comparative example 2
Similar to the procedure of example 1, except that: the passivation time was 10 days. After restoration, the leaching concentrations of Cu, Cd and Pb in the slag are respectively reduced from 9.92mg/L, 0.51mg/L and 3.56mg/L to 9.76mg/L, 0.53mg/L and 3.38mg/L, which are respectively reduced by 1.61%, -3.92% and 5.06% after the enterococcus LZU-1 bacterial liquid is independently added for restoration; after bacterial liquid is added into slag containing 10% compost sludge for restoration, the leachable concentrations of Cu, Cd and Pb in the slag are respectively reduced to 5.28mg/L, 0.44mg/L and 1.35mg/L, and the passivation efficiencies respectively reach 46.77%, 13.73% and 62.08%.
Comparative example 3
Similar to the procedure of example 2, except that: the passivation time was 10 days. After the restoration, after the enterococcus LZU-1 bacterial liquid is independently added for restoration, the leachable concentrations of Cd and Pb in the sediments are respectively reduced from 2.42mg/L and 0.30mg/L to 2.26mg/L and 0.23mg/L, and are respectively reduced by 6.61 percent and 23.33 percent; after bacterial liquid is added into sediments containing 10% compost sludge for restoration, the leachable concentration of Cd and Pb is reduced to 2.22mg/L and 0.14mg/L, and the passivation efficiency respectively reaches 8.26% and 53.33%.
In the above, the embodiment of the invention provides a method for in-situ passivation of heavy metals in solid waste generated by mining, which is characterized in that enterococcus LZU-1 bacterial liquid is combined with compost sludge to passivate the heavy metals in the solid waste generated by mining, the preparation process of the enterococcus LZU-1 bacterial liquid and the composition of the cementing liquid are defined, and the mixture of the solid waste and the compost sludge generated by mining per gram corresponds to the volume of the enterococcus LZU-1 bacterial liquid and the volume of the cementing liquid and the time required by passivation, because in the passivation and repair process, such as the addition amount of the bacterial liquid, the efficiency of forming carbonate precipitates by the heavy metals is influenced; the concentration of calcium chloride in the cementing liquid influences the efficiency of carbonate precipitation on the one hand and the dissolution efficiency of heavy metals on the other hand, and when the calcium chloride is too high, the dissolution amount of the heavy metals is increased, and the repair efficiency is reduced. The compost sludge is low in addition amount, the porosity of a polluted medium cannot be effectively improved, oxygen required by microorganisms is insufficient, and a good nutrient environment cannot be provided for the microorganisms. However, the compost sludge content is too high, so that the bulkiness of the polluted medium is greatly increased, the volume of the polluted medium is greatly increased, and the volume of the polluted medium can be increased by more than 50% by estimation when 20% of compost sludge is added. Based on the principle of reducing the volume of pollutants as much as possible, the method does not suggest increasing the amount of compost sludge without limitation. The passivation process of the microorganisms to the heavy metals is a complex dissolution and stabilization process, and when the passivation time is short, the microorganisms are not fully contacted with the heavy metal ions, the formed products are unstable, and the best repair effect is difficult to achieve. Therefore, according to the embodiment of the invention, by improving and optimizing the passivation process, the microorganisms and the compost sludge jointly convert the heavy metals from the active form to the stable form under the stimulation action of the compost sludge on the microorganisms by utilizing the method for passivating the heavy metals in the solid wastes generated by mining in the in-situ passivation mine, so that the passivation effect on the heavy metals such as copper (Cu), cadmium (Cd), lead (Pb) and the like is achieved, and the leaching concentration of the heavy metals from pollutants and the threat of the heavy metals to water and the surrounding ecological environment are reduced.
Compared with the prior art, the scheme provided by the embodiment of the invention has the following characteristics:
(1) the enterococcus LZU-1 has the urease production function, has the passivation effect on nonferrous metal slag and heavy metal in mine sewage canal sediments, is low in cost and environment-friendly, and has important significance in mine heavy metal remediation.
(2) When the microorganisms passivate heavy metals, compost sludge is added into pollutants, so that the passivation effect on the heavy metals can be effectively improved. The compost sludge is solid waste generated by an urban sewage treatment plant, and can achieve the purpose of resource utilization when being used in the passivation technology of mine solid waste heavy metal.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A method for passivating heavy metals in solid waste generated by mining in situ is characterized in that enterococcus LZU-1 bacterial liquid is combined with compost sludge to passivate the heavy metals in the solid waste generated by mining;
wherein the heavy metal is selected from at least one of Cu, Cd and Pb;
the preservation number of the enterococcus LZU-1 is CGMCC 22622.
2. The method of claim 1, comprising the steps of: and applying the enterococcus LZU-1 bacterial liquid and the cementing liquid to a mixture of solid waste and compost sludge generated by mining, and culturing at room temperature to passivate heavy metals in the solid waste generated by mining.
3. The method of claim 2The method is characterized in that the preparation process of the enterococcus LZU-1 bacterial liquid comprises the following steps: inoculating activated enterococcus LZU-1 in nutrient broth culture medium, performing shaking culture at room temperature, and diluting the enriched culture solution with water to OD6000.8 to 1.2;
preferably, the purified enterococcus LZU-1 is cultured in a shaker at 25-35 ℃ and 100-600Is 0.8-1.2.
4. The method according to claim 3, characterized in that the nutrient broth medium composition is: 4-6g/L of sodium chloride, 2-4g/L of beef extract and 8-12g/L of peptone.
5. The method of claim 2, wherein the composition of the cementing fluid is: 0.01-0.1mol/L of calcium chloride, 0.2-0.7mol/L of urea, 4-6g/L of sodium chloride, 2-4g/L of beef extract and 8-12g/L of peptone.
6. The method according to claim 2, wherein the mixture of solid waste and compost sludge produced in mining per gram corresponds to 0.1-1mL of the enterococcus LZU-1 bacterial liquid and 0.1-1mL of the cementing liquid.
7. The method of claim 2, wherein the compost sludge is obtained by composting municipal sewage treatment plant sludge and the compost sludge is comminuted into particles having a particle size of less than 2mm before being used for remediation.
8. The method of claim 2, wherein the solid waste from mining is any one of non-ferrous slag and sewer sediment.
9. The method according to claim 2, wherein the enterococcus LZU-1 bacterium solution and the cementing solution are poured into the sample and mixed and cultured for 8-12 days.
10. The method of claim 9, further comprising: pouring the enterococcus LZU-1 bacterial liquid and the cementing liquid into the sample which is passivated for 8-12 days, and continuing to perform passivating culture for 8-12 days.
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Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005279442A (en) * | 2004-03-29 | 2005-10-13 | Japan Science & Technology Agency | Method of treating livestock waste |
| WO2007111479A1 (en) * | 2006-03-28 | 2007-10-04 | Korea Forest Research Institute | Soil improver with white-rot fungi and methods of using thereof for plant restoration and recover of heavy metals contaminated soil around abandoned mine area |
| US20170008052A1 (en) * | 2015-07-07 | 2017-01-12 | Korea Institute Of Geoscience And Mineral Resources (Kigam) | Method for remediating contaminated soil using microorganism strain having ability to produce urease |
| CN106905980A (en) * | 2017-03-02 | 2017-06-30 | 北京绿安创华环保科技股份有限公司 | A kind of heavy metal sewage sludge soil-repairing agent, preparation method and its application in sludge is dissolved |
| US20180119185A1 (en) * | 2012-07-17 | 2018-05-03 | Arizona Board of Regents on behalf of Arizona Stat e University | Cementation Methods |
| CN108862948A (en) * | 2018-06-19 | 2018-11-23 | 浙江工业大学 | Solidify the method for the cementing electroplating sludge containing heavy metal using sporosarcina |
| CN108893419A (en) * | 2018-05-24 | 2018-11-27 | 苏州中益世纪生态环境设计研究有限公司 | Microbial strains and its screening technique and the application in processing heavy-metal contaminated soil |
| US20190210924A1 (en) * | 2016-06-24 | 2019-07-11 | Universitetet I Oslo | Bio-catalytic calcium carbonate cementation |
| CN111234835A (en) * | 2020-02-17 | 2020-06-05 | 兰州大学 | Repairing agent for in-situ repairing heavy metal contaminated soil and in-situ repairing method |
| CN111672900A (en) * | 2020-06-01 | 2020-09-18 | 合肥工业大学 | Method for restoring heavy metal pollution of soil by microorganism-induced calcium carbonate precipitation |
| CN111689667A (en) * | 2020-06-22 | 2020-09-22 | 北京柏嘉伦环保科技有限公司 | Method for degrading sludge heavy metal by using extreme microorganisms, product and application |
| AU2020102540A4 (en) * | 2020-09-30 | 2020-11-19 | Beijing baijialun Environmental Protection Technology Co., Ltd | Method, product and application for degrading sludge heavy metals by extreme microorganisms |
| CN112387778A (en) * | 2020-10-13 | 2021-02-23 | 湖南省林业科学院 | Method for restoring heavy metal contaminated soil by combining sludge biochar immobilized bacteria with plants |
| CN113333460A (en) * | 2021-06-24 | 2021-09-03 | 兰州大学 | Method for in-situ remediation of heavy metal contaminated soil |
| CN113578954A (en) * | 2021-07-28 | 2021-11-02 | 同济大学 | Method for controlling heavy metal leaching pollution of mine by using humified product |
-
2022
- 2022-01-06 CN CN202210010072.9A patent/CN114308987B/en active Active
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005279442A (en) * | 2004-03-29 | 2005-10-13 | Japan Science & Technology Agency | Method of treating livestock waste |
| WO2007111479A1 (en) * | 2006-03-28 | 2007-10-04 | Korea Forest Research Institute | Soil improver with white-rot fungi and methods of using thereof for plant restoration and recover of heavy metals contaminated soil around abandoned mine area |
| US20180119185A1 (en) * | 2012-07-17 | 2018-05-03 | Arizona Board of Regents on behalf of Arizona Stat e University | Cementation Methods |
| US20170008052A1 (en) * | 2015-07-07 | 2017-01-12 | Korea Institute Of Geoscience And Mineral Resources (Kigam) | Method for remediating contaminated soil using microorganism strain having ability to produce urease |
| US20190210924A1 (en) * | 2016-06-24 | 2019-07-11 | Universitetet I Oslo | Bio-catalytic calcium carbonate cementation |
| CN106905980A (en) * | 2017-03-02 | 2017-06-30 | 北京绿安创华环保科技股份有限公司 | A kind of heavy metal sewage sludge soil-repairing agent, preparation method and its application in sludge is dissolved |
| CN108893419A (en) * | 2018-05-24 | 2018-11-27 | 苏州中益世纪生态环境设计研究有限公司 | Microbial strains and its screening technique and the application in processing heavy-metal contaminated soil |
| CN108862948A (en) * | 2018-06-19 | 2018-11-23 | 浙江工业大学 | Solidify the method for the cementing electroplating sludge containing heavy metal using sporosarcina |
| CN111234835A (en) * | 2020-02-17 | 2020-06-05 | 兰州大学 | Repairing agent for in-situ repairing heavy metal contaminated soil and in-situ repairing method |
| CN111672900A (en) * | 2020-06-01 | 2020-09-18 | 合肥工业大学 | Method for restoring heavy metal pollution of soil by microorganism-induced calcium carbonate precipitation |
| CN111689667A (en) * | 2020-06-22 | 2020-09-22 | 北京柏嘉伦环保科技有限公司 | Method for degrading sludge heavy metal by using extreme microorganisms, product and application |
| AU2020102540A4 (en) * | 2020-09-30 | 2020-11-19 | Beijing baijialun Environmental Protection Technology Co., Ltd | Method, product and application for degrading sludge heavy metals by extreme microorganisms |
| CN112387778A (en) * | 2020-10-13 | 2021-02-23 | 湖南省林业科学院 | Method for restoring heavy metal contaminated soil by combining sludge biochar immobilized bacteria with plants |
| CN113333460A (en) * | 2021-06-24 | 2021-09-03 | 兰州大学 | Method for in-situ remediation of heavy metal contaminated soil |
| CN113578954A (en) * | 2021-07-28 | 2021-11-02 | 同济大学 | Method for controlling heavy metal leaching pollution of mine by using humified product |
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