CN103449696A - Method for improving oil sludge separating and processing efficiency and bacterial strain used for method - Google Patents
Method for improving oil sludge separating and processing efficiency and bacterial strain used for method Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention belongs to the environment-friendly field, and discloses a method for improving oil sludge separating and processing efficiency and a bacterial strain used for the method. The method for improving the oil sludge separating and processing efficiency comprises the following steps: (1), sampling and analyzing processed oil sludge; (2), determining microorganism nutrients to be added according to components and content of a nitrogen element and a phosphor element in the processed oil sludge; (3), adding a mixed culture of candida lipolytica Y-57 and pseudomonas putida P-101 and the microorganism nutrients into the to-be-processed oil sludge, stirring, ventilating and culturing for over 8 hours under the condition with pH of 6-10 under a temperature of 8 DEG C-45 DEG C to separate oil and mud in the oil sludge. According to the invention, a microorganism treatment method is adopted for separating the oil and the mud in the oil sludge to achieve an effect of an oil resource recovery rate; moreover, a chemical agent which generates influence to the environment is not added, so that secondary pollution is not generated. Resourceful treatment is carried out on oil-containing mud to obtain a certain economic benefit, and therefore, the method is an economical and effective oil sludge treating technology.
Description
Technical field
The invention belongs to field of Environment Protection, relate to a kind of method of greasy filth separation treatment efficiency and bacterial strain of use thereof of improving.
Background technology
In oil production, accumulating and the refining course of processing, often produce the greasy filth that some oleaginousness are higher, if these greasy filth are stacked arbitrarily, will become the important source of pollution of oil field and surrounding enviroment.Oil is difficult to remove after entering soil, and both the spoiled soil structure, produced harm to microorganism and the edaphophyte ecosystem of soil self again.
The composition of oily sludge is extremely complicated, generally by oil-in-water, water-in-oil and suspended solid impurities, formed, contain a large amount of aging crude oil, wax, bituminous matter, colloid, suspended solid, bacterium, salt, sour gas, corrosion product etc., also comprise the water conditioners such as a large amount of flocculation agent of adding in production process, inhibiter, Scale inhibitors, sterilant, the methods such as the burning method in succession occurred in recent years, thermal desorption method, pyrogenic process, solidification method are processed oily sludge, because the reasons such as cost is high, crude oil can't reclaim, secondary pollution all fail to promote on a large scale.The direct landfill of oily sludge or solidify after landfill be the main method that current most oil field at home adopts, but the shortcoming of this method is to pollute easily to be transferred, likely polluted underground water etc., both wasted the valuable energy wherein, also likely causes environmental pollution; Burning method has and subtracts that to hold effect remarkable, processes safer, the advantage of elimination pathogenic bacteria, but burn, must in the special incinerator of setting up, carry out, energy consumption is high, facility investment is large, Technology is had relatively high expectations, and need to add ignition dope, has the secondary pollutions such as dust, waste gas.According to statistics, burn oily sludge per ton and need oil consumption 18.5kg, its processing cost is high, therefore cause energy dissipation also to be implemented simultaneously.Chemical method is suitable for oleaginousness at the oily sludge more than 5 ~ 10%, because its treating processes need to add chemical agent usually, need special treatment facility, complex disposal process, crude oil recovery is not thorough, has the problems such as waste residue and secondary waste water pollutent and is not generally promoted the use of.Bioremediation Technology also becomes the focus of China's research at present.Great majority all are devoted to study the high-effective microorganism of labile organic compound, make great efforts the more new high efficient petroleum degrading bacteria kind of exploitation.Because biological treating can accelerate the natural biology degradation rate by the optimization to environmental factors, be a kind of economy, the efficient and ecological cleaning technique that can bear.Therefore, the research field of bioremediation Technology has expanded to the improvement to underground water, soil, pollution of the sea such as solid waste, agricultural chemicals, oil at present.
The crude oil that microorganism be take in oily sludge is grown as carbon source, but produced simultaneously tensio-active agent cut oil makes more effectively degraded oil of microorganism, thereby reach, oil, water, silt in greasy filth separates and the purpose of contaminant degradation.The factor that affects oily sludge biodegrading is many and complicated, and in the kind of oil degradation microorganism, oily sludge, kind, component, physico-chemical property and the residing state etc. of oil are most important to the degraded of oily sludge.Therefore according to the greasy filth bioremediation of the oil degradation bacterial classification of certain specific place screening, foundation sometimes and be not suitable for the separating treatment of a ground greasy filth.
Summary of the invention
The purpose of this invention is to provide a kind of method that improves the greasy filth separation treatment efficiency.
Another object of the present invention is to provide the bacterial strain used in the method.
Purpose of the present invention can be achieved through the following technical solutions:
A kind of method that improves the greasy filth separation treatment efficiency comprises following steps:
(1) to processing the greasy filth sampling analysis;
(2) according to processing nitrogen element and phosphoric composition and the definite micro-nutrients added of content in greasy filth;
(3) to add mixed culture and micro-nutrients and/or the tensio-active agent of the pseudomonas putida P-101 that Candida lipolytica Y-57 that described deposit number is CGMCC No.5790 and deposit number are CGMCC No.5801 in pending greasy filth by the dosage of 0.01%-1wt%, stir, ventilate at PH=6-10, temperature 8-45 ℃ of lower 50-150rpm, cultivate more than 8 hours, the oil in greasy filth is separated with mud.
Wherein, describedly to processing greasy filth, carry out sampling analysis, comprise the greasy filth sample is carried out to analysis of physical and chemical property and/or endogenous microbes analysis, determine oil, mud accounting in greasy filth, obtain nitrogen element and phosphoric composition and content in local water, and in oil recovery and/or oil refining process, add some affect the chemical agent of microorganism growth, the quantity of aerobic-anaerobic microbe flora.
When in described greasy filth system for handling, total nitrogen concentration is less than 200mg/L, adds nitrogenous source to total nitrogen concentration and be at least 200mg/L; When in the greasy filth system for handling, total phosphorus concentration is less than 25mg/L, adds phosphorus source to total phosphorus concentration and be at least 25mg/L.
Described nitrogenous source micro-nutrients is ammonium chloride or urea, described phosphorus source micro-nutrients is phosphoric acid salt, and described tensio-active agent is selected from one or more of tween, sapn, rhamnolipid, alkylaryl polyglycol ether, alkyl polyglycol ether or alkylaryl sulphonate.
In the mixed culture of described Candida lipolytica Y-57 and pseudomonas putida P-101, the quantity of effective microbe is greater than 10
5cfu/mL.
Prepared by the mixed culture of described Candida lipolytica Y-57 and pseudomonas putida P-101: Candida lipolytica Y-57 and pseudomonas putida P-101 are inoculated in to fermention medium by the following method, at 30 ℃, stir 100-300rpm, air quantity 1:0.4-0.5, cultivate 16-48 hour under tank pressure 0.05-1.0mpa.
Described fermentative medium formula is: glucose 5-20 grams per liter, whiteruss 10-30 grams per liter, yeast extract paste 1-5.0 grams per liter, sal epsom 0.1-0.5 grams per liter, Repone K 0.1-5.0 grams per liter, potassium primary phosphate 1-5.0 grams per liter, urea 0.5-5.0 grams per liter, ammonium nitrate 0.5-5.0 grams per liter, tween-80 0.1-0.5 milliliter/liter, trace salt solution 1-3.0 milliliter/liter, the pH nature; Preferred glucose 10 grams per liters, whiteruss 15 grams per liters, yeast extract paste 3.0 grams per liters, sal epsom 0.2 grams per liter, Repone K 0.2 grams per liter, potassium primary phosphate 2.0 grams per liters, urea 1.0 grams per liters, ammonium nitrate 1.5 grams per liters, 0.5 milliliter/liter of tween-80,1.0 milliliters/liter of trace salt solution.
Wherein: every liter of trace salt solution contains zinc sulfate 11.0 grams, manganous sulfate 6.0 grams, ferrous sulfate 1.0 grams, EDTA-Na
27.3 gram, rose vitriol 0.3 gram, 40 milligrams, copper sulfate, 60 milligrams of boric acid, 1 milligram of potassiumiodide.
Candida lipolytica Y-57(Candida lipolytica), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation day is on February 24th, 2012, and deposit number is CGMCC No.5790.
Pseudomonas putida P-101(Pseudomonas putida), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation day is on February 24th, 2012, and deposit number is CGMCC No.5801.
The application of pseudomonas putida P-101 in the greasy filth separating treatment that the Candida lipolytica Y-57 that described deposit number is CGMCC No.5790 and/or described deposit number are CGMCC No.5801.
Beneficial effect:
The present invention adopts microbial treatment method, and oil in greasy filth is separated with mud, reaches the effect of oily resource recovery, and does not add the chemical agent that environment is exerted an influence, and does not produce secondary pollution.Sludge containing being carried out to recycling treatment, can obtain certain economic benefit, is a kind of cost-effective greasy filth treatment technology.
The present invention is directed to concrete greasy filth is detected, nitrogen element and phosphoric composition and the definite micro-nutrients added of content according to greasy filth self, promote the microbial growth and the metabolism that there is unique degraded heavy oil and produce the ability of tensio-active agent that add, have more specific aim.
Near the two high-efficiency degradation petroleum-type microorganisms that the Candida lipolytica Y-57 added in the present invention and pseudomonas putida P-101 Shi Cong restaurant, in trench greasy filth, screening obtains, this two strains bacterium is applied to greasy filth to be separated, improved the separating effect of oil and mud in greasy filth, and mud and (or) degradation effect of irreducible oil in water, oil-contg<2%(residual in mud is in dry mud).
Biomaterial preservation information
P-101, Classification And Nomenclature is pseudomonas putida (Pseudomonas putida), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, the preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, preservation day is on February 24th, 2012, and deposit number is CGMCC No.5801.
Y-57, Classification And Nomenclature is Candida lipolytica (Candida lipolytica), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, the preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, preservation day is on February 24th, 2012, and deposit number is CGMCC No.5790.
Embodiment
Embodiment 1
Near choosing the Yixing City restaurant, trench greasy filth is screened by the following method:
1.1 bacterial classification enrichment
In dextrose culture-medium in triangular flask, add certain greasy filth (2%) to put into shaking table,, cultivate 3 days, obtain the bacterial classification pregnant solution for 30 ℃.The dextrose culture-medium formula is: sodium-chlor 1g, calcium chloride 0.5g, dipotassium hydrogen phosphate 0.5g, potassium primary phosphate 0.5g, iron(ic) chloride 0.02g, ammonium nitrate 1g, magnesium sulfate heptahydrate, 1g yeast extract, 2g glucose, 1L water, PH 7.0-7.2; 110 ℃ of sterilizing 20min.
1.2 bacterial screening
The bacterial classification pregnant solution is diluted later again glucose solids culture medium flat plate (adding 2% agar in dextrose culture-medium)) upper line separation, in 30 ℃, cultivate 24 hours, picking on flat board well-grown single bacterium colony streak inoculation on the alkane flat board, in 30 ℃ of cultivations 48 hours, choose two maximum single bacterium colonies called after Y-57, P-101 respectively.
Embodiment 2
The Y-57 bacterial strain is carried out to biology and biochemical test, and this yeast YPD Agar is incubated at flat board (30 ℃) 3 days, and bacterium colony can grow to the 1-2 millimeter, and white is to cream-colored.The initial stage bacterium colony is smooth shape, through the Extending culture bacterium colony, gauffer occurs.While growing on solid or liquid nutrient medium, the rounded or oval of cellular form, cell also can elongate and be the false filamentous growth of class sometimes, and cell size is about 4-6 * 6-8 micron.
The Biolog microbe auto-analysis instrument that this yeast is produced with U.S. Biolog company is identified, is accredited as Candida lipolytica.
The P-101 bacterial strain is carried out to biology and biochemical test, the aerobic cultivation of this bacterium nutrient agar plate 24 hours, bacterial growth is vigorous, and bacterium colony is smooth, moistening, translucent faint yellow bacterium colony, produce strong smell, the negative bacillus of gramstaining, extremely give birth to many flagellums, and most bacterial strain flagellums are more than three.This bacterium obligate is aerobic, the breathing pattern metabolism, and decomposition glucose and wood sugar, utilize citritase, and the Phenylalanine dehydrogenase reaction is all negative.Utilize inositol, oxydase and the Terminal oxidase positive, the arginine dihydrolase positive, do not produce Polylevulosan, not gelatin hydrolysate, hydrolyzed starch not, the hydrolysis polychrom, egg yellow reaction feminine gender, Poly-β-hydroxybutyric Acid salt feminine gender, reduction nitrate, U.S. red negative, voges-Proskauer test feminine gender, indole test feminine gender; Can grow for 4 ℃, not grow for 41 ℃; Appropriate pH is 7-8.5.
The Biolog microbe auto-analysis instrument that this bacterium is produced with U.S. Biolog company is identified, is accredited as pseudomonas putida.
Candida lipolytica Y-57 of the present invention and pseudomonas putida P-101 on February 24th, 2012 in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, wherein, Candida lipolytica Y-57 deposit number is CGMCC NO.5790, and Classification And Nomenclature is: Candida lipolytica (Candida lipolytica); Pseudomonas putida P-101 deposit number is CGMCC NO.5801, and Classification And Nomenclature is: pseudomonas putida (Pseudomonas putida).
Embodiment 3
(1) get the greasy filth sample of North China Oilfield slurry tank, to processing the greasy filth sampling analysis: measuring oleaginousness according to " agricultural mud method for monitoring and analyzing " is 10%, adopt the ion method heavy metal, every heavy metal index all is less than pollutent control criterion (GB4284-84) in agricultural mud, adopting alkaline Potassium Persulphate to measure total nitrogen in greasy filth is 35mg/L, with in Ammonium Molybdate Spectrophotometric Method for Determination greasy filth total phosphorus 12mg/L.
(2) Candida lipolytica Y-57(CGMCC NO.5790) and pseudomonas putida P-10(CGMCC NO.5801) use respectively the LB substratum, at 30 ℃, the 200rpm shaking table is cultivated 24 hours, be seeded to fermention medium according to 5% inoculum size again, in 30 ℃, 200rpm stirs, and air quantity 1:0.4-0.5 cultivates 36 hours under tank pressure 0.05mpa.
Fermention medium (grams per liter): glucose 10, whiteruss 15, yeast extract paste 3.0, sal epsom 0.2, Repone K 0.2, potassium primary phosphate 2.0, urea 1.0, ammonium nitrate 1.5, tween-80 0.5ml, trace salt solution 1.0ml.The pH nature.
Wherein: every liter of trace salt solution contains zinc sulfate 11.0 grams, manganous sulfate 6.0 grams, ferrous sulfate 1.0 grams, EDTA-Na
27.3 gram, rose vitriol 0.3 gram, 40 milligrams, copper sulfate, 60 milligrams of boric acid, 1 milligram of potassiumiodide.
(3) to adding 0.1wt%(in pending greasy filth by the greasy filth weighing scale) mixed culture of the described deposit number Candida lipolytica Y-57 that is CGMCC No.5790 and the deposit number pseudomonas putida P-101 that is CGMCC No.5801; According to processing nitrogen element and phosphoric composition and definite potassium primary phosphate 60mg/L, urea 360mg/L and the tween-80 500mg/L of adding of content in greasy filth; Stir under PH=7.5,22 ℃ of temperature, 100rpm, ventilate, cultivate more than 8 hours, oil separates with mud, and oil can float on upper strata, and measuring residual oil-contg in mud according to " agricultural mud method for monitoring and analyzing " is that 1.7%(is in dry mud).
Claims (10)
1. a method that improves the greasy filth separation treatment efficiency is characterized in that comprising following steps:
(1) to processing the greasy filth sampling analysis;
(2) according to processing nitrogen element and phosphoric composition and definite micro-nutrients and/or the tensio-active agent added of content in greasy filth;
(3) to add mixed culture and micro-nutrients and/or the tensio-active agent of the pseudomonas putida P-101 that Candida lipolytica Y-57 that described deposit number is CGMCC No.5790 and deposit number are CGMCC No.5801 in pending greasy filth by the dosage of 0.01%-1wt%, stir, ventilate at pH=6-10, temperature 8-45 ℃ of lower 50-150rpm, cultivate more than 8 hours, the oil in greasy filth is separated with mud.
2. the method for raising greasy filth separation treatment efficiency according to claim 1, it is characterized in that describedly to processing greasy filth, carrying out sampling analysis, comprise the greasy filth sample is carried out to analysis of physical and chemical property and/or endogenous microbes analysis, determine oil, mud accounting in greasy filth, obtain nitrogen element and phosphoric composition and content in local water, and in oil recovery and/or oil refining process, add some affect the chemical agent of microorganism growth, the quantity of aerobic-anaerobic microbe flora.
3. the method for raising greasy filth separation treatment efficiency according to claim 1, while it is characterized in that in described processing greasy filth, ammonia nitrogen concentration is less than 200mg/L, add the nitrogenous source micro-nutrients to total nitrogen concentration for being at least 200mg/L; When in the greasy filth system for handling, total phosphorus concentration is less than 25mg/L, adds phosphorus source micro-nutrients to total phosphorus concentration and be at least 25mg/L.
4. the method for raising greasy filth separation treatment efficiency according to claim 3, it is characterized in that described nitrogenous source micro-nutrients is ammonium chloride or urea, described phosphorus source micro-nutrients is phosphoric acid salt, and described tensio-active agent is selected from one or more of tween, sapn, rhamnolipid, alkylaryl polyglycol ether, alkyl polyglycol ether or alkylaryl sulphonate.
5. the method for raising greasy filth separation treatment efficiency according to claim 1, is characterized in that the quantity of effective microbe in the mixed culture of described Candida lipolytica Y-57 and pseudomonas putida P-101 is greater than 10
5cfu/mL.
6. the method for raising greasy filth separation treatment efficiency according to claim 5, prepared by the mixed culture that it is characterized in that described Candida lipolytica Y-57 and pseudomonas putida P-101: Candida lipolytica Y-57 and pseudomonas putida P-101 are inoculated in to fermention medium by the following method, at 30 ℃, stir 100-300rpm, air quantity 1: 0.4-0.5, cultivate 16-48 hour under tank pressure 0.05-1.0mpa; Described fermentative medium formula is: glucose 5-20 grams per liter, whiteruss 10-30 grams per liter, yeast extract paste 1-5.0 grams per liter, sal epsom 0.1-0.5 grams per liter, Repone K 0.1-5.0 grams per liter, potassium primary phosphate 1-5.0 grams per liter, urea 0.5-5.0 grams per liter, ammonium nitrate 0.5-5.0 grams per liter, tween-80 0.1-0.5 milliliter/liter, trace salt solution 1-3.0 milliliter/liter, the pH nature; Every liter of described trace salt solution contains zinc sulfate 11.0 grams, manganous sulfate 6.0 grams, ferrous sulfate 1.0 grams, EDTA-Na
27.3 gram, rose vitriol 0.3 gram, 40 milligrams, copper sulfate, 60 milligrams of boric acid, 1 milligram of potassiumiodide.
7. the method for raising greasy filth separation treatment efficiency according to claim 6, it is characterized in that described fermentative medium formula is: glucose 10 grams per liters, whiteruss 15 grams per liters, yeast extract paste 3.0 grams per liters, sal epsom 0.2 grams per liter, Repone K 0.2 grams per liter, potassium primary phosphate 2.0 grams per liters, urea 1.0 grams per liters, ammonium nitrate 1.5 grams per liters, 0.5 milliliter/liter of tween-80,1.0 milliliters/liter of trace salt solution.
8. Candida lipolytica Y-57(Candida lipolytica), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation day is on February 24th, 2012, and deposit number is CGMCC No.5790.
9. pseudomonas putida P-101(Pseudomonas putida), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation day is on February 24th, 2012, and deposit number is CGMCC No.5801.
10. Candida lipolytica Y-57 claimed in claim 8 and/or the application of pseudomonas putida P-101 claimed in claim 9 in the greasy filth separating treatment.
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| CN111547969A (en) * | 2020-05-30 | 2020-08-18 | 榕知(杭州)信息技术有限公司 | Method for degrading hydrocarbon pollutants in oil-containing sludge of oil field by microorganisms |
| CN114477674A (en) * | 2022-02-15 | 2022-05-13 | 江苏博大环保股份有限公司 | Oil sludge bioremediation system |
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