CN114304393A - Plant composition and preparation method and application thereof - Google Patents
Plant composition and preparation method and application thereof Download PDFInfo
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- CN114304393A CN114304393A CN202111575812.5A CN202111575812A CN114304393A CN 114304393 A CN114304393 A CN 114304393A CN 202111575812 A CN202111575812 A CN 202111575812A CN 114304393 A CN114304393 A CN 114304393A
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- vitamin
- carnosol
- carnosic acid
- feed
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- 239000000203 mixture Substances 0.000 title claims abstract description 56
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- QRYRORQUOLYVBU-VBKZILBWSA-N carnosic acid Chemical compound CC([C@@H]1CC2)(C)CCC[C@]1(C(O)=O)C1=C2C=C(C(C)C)C(O)=C1O QRYRORQUOLYVBU-VBKZILBWSA-N 0.000 claims abstract description 135
- 229930003316 Vitamin D Natural products 0.000 claims abstract description 46
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 claims abstract description 46
- 235000019166 vitamin D Nutrition 0.000 claims abstract description 46
- 239000011710 vitamin D Substances 0.000 claims abstract description 46
- 150000003710 vitamin D derivatives Chemical class 0.000 claims abstract description 46
- 229940046008 vitamin d Drugs 0.000 claims abstract description 46
- XUSYGBPHQBWGAD-PJSUUKDQSA-N Carnosol Chemical compound CC([C@@H]1C2)(C)CCC[C@@]11C(=O)O[C@@H]2C2=C1C(O)=C(O)C(C(C)C)=C2 XUSYGBPHQBWGAD-PJSUUKDQSA-N 0.000 claims abstract description 45
- MMFRMKXYTWBMOM-UHFFFAOYSA-N Carnosol Natural products CCc1cc2C3CC4C(C)(C)CCCC4(C(=O)O3)c2c(O)c1O MMFRMKXYTWBMOM-UHFFFAOYSA-N 0.000 claims abstract description 45
- 235000004654 carnosol Nutrition 0.000 claims abstract description 45
- 241000196324 Embryophyta Species 0.000 claims abstract description 41
- 239000010668 rosemary oil Substances 0.000 claims abstract description 3
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- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 description 3
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- GUOCOOQWZHQBJI-UHFFFAOYSA-N 4-oct-7-enoxy-4-oxobutanoic acid Chemical compound OC(=O)CCC(=O)OCCCCCCC=C GUOCOOQWZHQBJI-UHFFFAOYSA-N 0.000 description 2
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- 210000000481 breast Anatomy 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
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- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 description 1
- RVBUGGBMJDPOST-UHFFFAOYSA-N 2-thiobarbituric acid Chemical compound O=C1CC(=O)NC(=S)N1 RVBUGGBMJDPOST-UHFFFAOYSA-N 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 235000019743 Choline chloride Nutrition 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 102000006587 Glutathione peroxidase Human genes 0.000 description 1
- 108700016172 Glutathione peroxidases Proteins 0.000 description 1
- AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 description 1
- 206010061598 Immunodeficiency Diseases 0.000 description 1
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- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
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- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
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- 102100033220 Xanthine oxidase Human genes 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
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- 229940054666 biotin 0.15 mg Drugs 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
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- 229940088129 calcium pantothenate 10 mg Drugs 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229960003178 choline chloride Drugs 0.000 description 1
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
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- 239000011630 iodine Substances 0.000 description 1
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- 239000011669 selenium Substances 0.000 description 1
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Landscapes
- Fodder In General (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a plant composition and a preparation method and application thereof, and relates to the technical field of feeds. The invention provides a plant composition, which comprises the following components: carnosic acid, carnosol, vitamin D; the weight ratio of the carnosic acid to the carnosol to the vitamin D is as follows: carnosic acid: carnosol: vitamin D ═ 1-5:2-4: 1-5. According to the invention, through the compound research on the active components of rosemary, the growth performance and slaughter performance of the formed plant composition are greatly improved when the plant composition is applied to feed under the combined action of carnosic acid, carnosol and vitamin D. The inventor of the application also finds that the combination of rosemary oil, carnosic acid, carnosol and vitamin D can further improve the performance.
Description
Technical Field
The invention relates to the technical field of feeds, in particular to a plant composition and a preparation method and application thereof.
Background
Due to the abuse of antibiotics in livestock and poultry production, the incidence of diseases related to immunodeficiency and the like in the society is increased, and efficient and safe feed additives are sought to replace the antibiotics and become the development trend of livestock and poultry production. With the improvement of living standard of people, the requirements of people on the taste, the sense, the nutritive value and the like of meat food are higher and higher, and at present, no good plant composition can meet various requirements.
Rosemary, a perennial aromatic plant belonging to the family Labiatae, is currently grown around the world and can extract both water-soluble and fat-soluble antioxidants as two functional ingredients. At present, the application of rosemary extract in feed has been researched by a plurality of balms, but on one hand, the water solubility of the rosemary fat-soluble antioxidant is poor, so that the release of all efficacies of the rosemary fat-soluble antioxidant is influenced greatly in the feed preparation process, and the absorption of animals to the rosemary fat-soluble antioxidant is also influenced; on the other hand, the research on the rosemary extract as a feed additive is basically a single component, and the research on the rosemary extract as a feed additive is not very prominent from the viewpoint of effect, so that the research on the rosemary extract as a feed additive is hindered at present.
Disclosure of Invention
Based on the above, the invention aims to overcome the defects of the prior art and provide a plant composition and a preparation method and application thereof.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows: a botanical composition comprising the following components: carnosic acid, carnosol, vitamin D; the weight ratio of the carnosic acid to the carnosol to the vitamin D is as follows: carnosic acid: carnosol: vitamin D ═ 1-5:2-4: 1-5.
According to the invention, through the compound research on the active components of rosemary, the effect is greatly improved when the carnosic acid, the carnosol and the vitamin D act together to form the plant composition.
Preferably, the weight ratio of carnosic acid, carnosol and vitamin D is as follows: carnosic acid: carnosol: vitamin D ═ 2-4: 2-3: 2-3. After a great deal of creative experiments, the inventor of the application finds that the weight ratio of the carnosic acid, the carnosol and the vitamin D has great influence on the performance of the final plant composition, and when the weight ratio is adopted, the effect is better.
Preferably, the weight ratio of carnosic acid, carnosol and vitamin D is as follows: carnosic acid: carnosol: vitamin D ═ 3: 2: 2. after a great deal of creative experiments, the inventor of the application finds that the weight ratio of the carnosic acid, the carnosol and the vitamin D has great influence on the performance of the final plant composition, and when the weight ratio is adopted, the effect is optimal.
Preferably, the botanical composition further comprises rosemary essential oil; the total weight ratio of the rosemary essential oil to the carnosic acid to the carnosol to the vitamin D is as follows: rosemary essential oil: the total weight of carnosic acid, carnosol, vitamin D is 1: 3-7. After a large number of creative experiments, the inventor of the application finds that the rosemary essential oil can be synergistically synergistic with the components, and the effect is better. Further preferably, the total weight ratio of rosemary essential oil to carnosic acid, carnosol and vitamin D is as follows: rosemary essential oil: the total weight of carnosic acid, carnosol, vitamin D is 1: 5. after a large number of creative experiments, the inventor of the application finds that when the total weight ratio of the rosemary essential oil to the carnosic acid to the carnosol to the vitamin D is the above components, the effect is optimal.
In addition, the invention provides microcapsule powder containing the plant composition, which comprises the following components in parts by weight: 2-10 parts of plant composition, 35-50 parts of wall material and 2-5 parts of emulsifier. Preferably, the wall material is at least one of Arabic gum and sodium starch octenyl succinate, and the emulsifier is at least one of soybean lecithin and glyceryl monostearate.
In addition, the present application provides a method for preparing the microcapsule powder, comprising the steps of: adding water into the wall material, and uniformly stirring to obtain a mixture A; and (3) uniformly mixing the plant composition and the emulsifier, adding the mixture into the mixture A, stirring, homogenizing, and performing spray drying to obtain the microcapsule powder.
Further, the invention provides application of the microcapsule powder in feed. The inventor of the application finds that the microcapsule powder has great influence on the growth performance of animals and the like by applying the microcapsule powder to the feed.
In addition, the invention provides a feed containing the plant composition, and the addition amount of the microcapsule powder in the feed is 180-320 mg/Kg. Preferably, the addition amount of the microcapsule powder in the feed is 250-300 mg/Kg. Further preferably, the addition amount of the microcapsule powder in the feed is 275 mg/Kg. After a great deal of creative experiments, the inventor of the application finds that the addition amount of the microcapsule powder in the feed has great influence on the growth performance of animals, and when the addition amount of the microcapsule powder in the feed is 275mg/Kg, the growth performance reaches the best.
Compared with the prior art, the invention has the beneficial effects that: according to the invention, through the compound research on the active components of rosemary, the growth performance and slaughter performance of the embedded plant composition are greatly improved under the combined action of carnosic acid, carnosol and vitamin D when the plant composition is applied to the feed. The inventor of the application also finds that the combination of rosemary oil, carnosic acid, carnosol and vitamin D can further improve the performance.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.
In the examples, the experimental methods used were all conventional methods unless otherwise specified, and the materials, reagents and the like used were commercially available without otherwise specified.
The plant composition provided by the invention is prepared from the following pure substances in proportion, and is not limited to the following materials: carnosic acid: 20% HPLC; carnosol: 10% HPLC; vitamin D: 99 percent; rosemary essential oil: 98 percent; is a self-owned product or a common product sold in the market;
the microcapsule powder can be applied to various feeds, and the inventor only lists the application of the microcapsule powder in chicken feeds; the invention uses the microcapsule powder in the chicken feed, the formula of the basic feed is shown in the following table 1, the basic feed used by the invention refers to NRC1994 and national chicken feeding standard NY/T33-2004:
TABLE 1 basic diet and nutritional ingredients (air drying standard)
The premix provides vitamin A14000 IU and vitamin D for each kilogram of diet32800 IU, E23.80mg vitamin K32.0mg, vitamin B12.2mg, vitamin B28 mg, nicotinic acid 30mg, choline chloride 400mg, calcium pantothenate 10mg, VB63mg, biotin 0.15mg, folic acid 1.00mg, VB120.013mg, iron 80mg, copper 8.0mg, manganese 110mg, zinc 65mg, iodine 1.5mg, selenium 0.3 mg.
Examples 1 to 12 and comparative examples 1 to 8
The plant compositions of examples 1 to 12 according to the invention were selected as shown in Table 2 below, and the plant compositions of comparative examples 1 to 8 were selected as shown in Table 3 below:
TABLE 2
TABLE 3
The preparation method of the antioxidant microcapsule powder comprises the following steps: adding water at 70 ℃ into a wall material (a compound of Arabic gum and sodium starch octenyl succinate, wherein the Arabic gum is the starch sodium octenyl succinate in a ratio of 5:2 and 50 parts), and uniformly stirring to obtain a mixture A; uniformly mixing the plant composition (5 parts) and an emulsifier (a compound of soybean lecithin and glyceryl monostearate, wherein the soybean lecithin is the glyceryl monostearate which is 5:1, 2 parts), adding the mixture into the mixture A, stirring, homogenizing, and carrying out spray drying to obtain the microcapsule powder; the invention discloses a conventional processing method of microcapsule powder, such as homogenization, spray drying and the like.
The selection of the components and the parts by weight of the plant composition of the comparative example 8 of the invention are completely the same as the selection of the plant composition of the example 1, the preparation method of the antioxidant microcapsule powder is different from the example, and the preparation method of the plant composition of the comparative example 8 of the invention is as follows: mixing carnosic acid, carnosol and vitamin D to obtain a plant composition;
effect verification
The test process comprises the following steps: 200 chickens which are hatched out of shells of 1 day old in the same batch are selected, and are randomly divided into 20 groups, wherein each group comprises 10 chickens (male half and female half). The test is set for 0-12 weeks, and the flat culture is carried out by adopting thick padding, and the food and the water are taken freely: the illumination time is 0-1 week old, the illumination time is 23-24h/d, the illumination time is 2-3 weeks old, the illumination time is 20-16h/d, the illumination time is 4-12 weeks old, and natural illumination is adopted;
and (3) measuring a growth index: during the raising of the test chickens, the daily feed intake was recorded, and the whole group was weighed on an empty stomach for each test group at each week's age, thereby calculating the daily feed intake (ADFI), daily gain (ADG), and feed-weight ratio (F/G). Average Daily Feed Intake (ADFI) — (feed consumption/(number of test days) —, Average Daily Gain (ADG) — gain/(number of test days) —, feed consumption/gain);
when the chickens are raised to 12 weeks old, 2 test chickens with the weight close to the average weight are selected from each group, one male chicken and one female chicken are selected, the chickens are fasted and weighed 6 hours before slaughtering, and pectoralis muscles and leg muscles are taken after slaughtering to measure pectoralis muscle rate and leg muscle rate indexes;
collecting blood of wing artery before slaughtering, placing in a centrifuge tube added with anticoagulant, standing for 1-2h, centrifuging at 3000 and 3500r/min for 5-10min, and separating serum to obtain relevant indexes to be measured. The activity of glutathione peroxidase (GSH-Px) is detected by a colorimetric method, the activity of serum total superoxide dismutase (T-SOD) is detected by a xanthine oxidase method (hydroxylamine method), and the content of Malondialdehyde (MDA) is detected by a thiobarbituric acid method (TBA). The kit for detection is commercially available, and the specific method refers to the kit specification;
and (3) test results: as shown in tables 4, 5 and 6;
TABLE 4
TABLE 5
TABLE 6
As can be seen from Table 4, the results of weighing the chicks during the feeding period of the chicks fed with the feed supplemented with the plant composition of the present invention showed a significant improvement in the performance in each respect. The invention researches the influence of the synergy of the carnosic acid, the carnosol and the vitamin D on the growth performance, and discovers that when the weight ratio of the carnosic acid to the carnosol to the vitamin D is as follows: carnosic acid: carnosol: vitamin D ═ 3: 2: 2, the growth performance is best; the inventor of the application finds that the growth performance is obviously improved by adding the rosemary essential oil, and the rosemary essential oil can be synergized with the components. The inventor also researches the influence of the dosage of the plant composition on the growth performance of the chicks, and when the dosage of the plant composition in the feed is 275mg/Kg, the growth performance is optimal. From the effects of comparative examples 1-7, carnosic acid, carnosol, vitamin D according to the present invention have synergistic effects as feed, and in the absence of any one of them, weight gain of chickens is significantly reduced and the feed-to-weight ratio is increased. The comparative example 8 is a plant composition which is not subjected to embedding treatment, and the antioxidant microcapsule powder prepared by the invention has obvious improvement on the growth performance of chicks from the final effect.
As can be seen from table 5, the breast muscle rate and the leg muscle rate of the chicks were significantly increased after eating the feed supplemented with the plant composition of the present invention. When the weight ratio of the carnosic acid, the carnosol and the vitamin D is as follows: carnosic acid: carnosol: vitamin D ═ 3: 2: at time 2, pectoral and leg muscle rates were optimized. The addition of the rosemary essential oil has obvious improvement on the chest muscle rate and the leg muscle rate, and the rosemary essential oil can be synergistically synergistic with the components. Comparative example 8 is a plant composition without embedding treatment, and from the final effect, the antioxidant microcapsule powder prepared by the present invention has significant improvement in pectoral muscle rate and leg muscle rate. The inventor of the application also researches the influence of the dosage of the plant composition on the pectoral muscle rate and the leg muscle rate, and the pectoral muscle rate and the leg muscle rate are optimal when the dosage of the plant composition in the feed is 275 mg/Kg. From the effects of comparative examples 1 to 7, carnosic acid, carnosol and vitamin D of the present invention have synergistic effects as a feed, and the rate of breast muscle and the rate of leg muscle are significantly decreased in the absence of any one of them.
As can be seen from table 6, the antioxidant activity of the chicks was significantly improved after eating the feed containing the plant composition of the present invention. When the weight ratio of the carnosic acid, the carnosol and the vitamin D is as follows: carnosic acid: carnosol: vitamin D ═ 3: 2: 2, the oxidation resistance is optimal. The addition of the rosemary essential oil has obvious improvement on the antioxidation performance, and the rosemary essential oil can be synergistic with the components. Comparative example 8 is a plant composition without embedding treatment, and the antioxidant microcapsule powder prepared according to the present invention has a significant improvement in antioxidant property from the viewpoint of the final effect. The inventor of the application also researches the influence of the dosage of the plant composition on the anti-oxidation performance, and the anti-oxidation performance is optimal when the dosage of the plant composition in the feed is 275 mg/Kg. From the effects of comparative examples 1 to 7, carnosic acid, carnosol and vitamin D according to the present invention have synergistic effects as a feed, and the antioxidant properties of none of them are significantly reduced.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (10)
1. A botanical composition, comprising the following components: carnosic acid, carnosol, vitamin D; the weight ratio of the carnosic acid to the carnosol to the vitamin D is as follows: carnosic acid: carnosol: vitamin D ═ 1-5:2-4: 1-5.
2. The botanical composition of claim 1, wherein the weight ratio of carnosic acid, carnosol, vitamin D is: carnosic acid: carnosol: vitamin D ═ 2-4: 2-3: 2-3.
3. The botanical composition of claim 1, wherein the weight ratio of carnosic acid, carnosol, vitamin D is: carnosic acid: carnosol: vitamin D ═ 3: 2: 2.
4. the botanical composition according to any of claims 1-3, further comprising rosemary oil; the total weight ratio of the rosemary essential oil to the carnosic acid to the carnosol to the vitamin D is as follows: rosemary essential oil: the total weight of carnosic acid, carnosol, vitamin D is 1: 3-7; preferably, the total weight ratio of rosemary essential oil to carnosic acid, carnosol and vitamin D is as follows: rosemary essential oil: the total weight of carnosic acid, carnosol, vitamin D is 1: 5.
5. a microcapsule powder comprising the plant composition according to any one of claims 1 to 4, comprising the following components in parts by weight: 2-10 parts of plant composition, 35-50 parts of wall material and 2-5 parts of emulsifier.
6. The microcapsule powder according to claim 5, wherein the preparation method of the microcapsule powder comprises the steps of: adding water into the wall material, and uniformly stirring to obtain a mixture A; and (3) uniformly mixing the plant composition and the emulsifier, adding the mixture into the mixture A, stirring, homogenizing, and performing spray drying to obtain the microcapsule powder.
7. The use of the microcapsule powder of claim 5 in feed.
8. Feed, which is characterized by comprising the microcapsule powder as described in claim 15, wherein the addition amount of the microcapsule powder in the feed is 180-320 mg/Kg.
9. The feed as claimed in claim 8, wherein the amount of the microcapsule powder added in the feed is 250-300 mg/Kg.
10. The feed of claim 9, wherein the amount of the microencapsulated powder added to the feed is 275 mg/Kg.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115282635A (en) * | 2022-08-03 | 2022-11-04 | 湖南植之源农牧有限公司 | Rosemary crude extract and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2618380A1 (en) * | 2008-01-23 | 2009-07-23 | Yves Marchand | Feed additive and uses thereof |
| WO2020127681A1 (en) * | 2018-12-21 | 2020-06-25 | Centre National De La Recherche Scientifique | Use of carnosol for increasing muscle protein synthesis |
| WO2020254391A1 (en) * | 2019-06-17 | 2020-12-24 | Impextraco Nv | Use of a composition for improving slaughtering and/or meat quality parameters |
| CN112189763A (en) * | 2020-10-21 | 2021-01-08 | 广州市信农生物科技有限公司 | Preparation process and application of plant essential oil microcapsule |
| WO2021245362A1 (en) * | 2020-06-05 | 2021-12-09 | Adisseo France S.A.S. | Vitamin a formulation |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2618380A1 (en) * | 2008-01-23 | 2009-07-23 | Yves Marchand | Feed additive and uses thereof |
| WO2020127681A1 (en) * | 2018-12-21 | 2020-06-25 | Centre National De La Recherche Scientifique | Use of carnosol for increasing muscle protein synthesis |
| WO2020254391A1 (en) * | 2019-06-17 | 2020-12-24 | Impextraco Nv | Use of a composition for improving slaughtering and/or meat quality parameters |
| WO2021245362A1 (en) * | 2020-06-05 | 2021-12-09 | Adisseo France S.A.S. | Vitamin a formulation |
| CN112189763A (en) * | 2020-10-21 | 2021-01-08 | 广州市信农生物科技有限公司 | Preparation process and application of plant essential oil microcapsule |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115282635A (en) * | 2022-08-03 | 2022-11-04 | 湖南植之源农牧有限公司 | Rosemary crude extract and preparation method and application thereof |
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