CN114288020A - Visible light illumination and near-infrared fluorescence operation navigation system based on shadowless lamp - Google Patents
Visible light illumination and near-infrared fluorescence operation navigation system based on shadowless lamp Download PDFInfo
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- CN114288020A CN114288020A CN202111512461.3A CN202111512461A CN114288020A CN 114288020 A CN114288020 A CN 114288020A CN 202111512461 A CN202111512461 A CN 202111512461A CN 114288020 A CN114288020 A CN 114288020A
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- 229910000530 Gallium indium arsenide Inorganic materials 0.000 claims description 6
- 239000011324 bead Substances 0.000 claims description 6
- 239000002096 quantum dot Substances 0.000 claims description 6
- 238000001356 surgical procedure Methods 0.000 claims description 5
- KXNLCSXBJCPWGL-UHFFFAOYSA-N [Ga].[As].[In] Chemical compound [Ga].[As].[In] KXNLCSXBJCPWGL-UHFFFAOYSA-N 0.000 claims description 3
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a visible light illumination and near-infrared fluorescence surgical navigation system based on a shadowless lamp. The invention is based on an LED shadowless lamp, a high-brightness near-infrared fluorescent probe and a near-infrared macroscopic imaging system. The system avoids the fluorescent signal submerged by the near-infrared band background light while providing visible light illumination for an operator by using the LED shadowless lamp, realizes the fluorescent excitation of the shadowless lamp by using the high-brightness near-infrared fluorescent probe, acquires the fluorescent image with high signal background ratio by using the high-sensitivity and low-noise near-infrared camera, and realizes the operation navigation by using the display device to display the near-infrared fluorescent image in real time. The invention avoids using an additional light source as exciting light like other near-infrared fluorescence operation navigation systems, avoids possible damage to eyes, simplifies the structure of illumination and reduces the cost. The invention has reliable performance and simple use, and has wide prospect in navigation operation.
Description
Technical Field
The invention relates to the technical field of fluorescence imaging and surgical navigation, in particular to a visible light illumination and near-infrared fluorescence surgical navigation system using a shadowless lamp.
Background
The near-infrared fluorescence imaging integrates the advantages of high spatial resolution, high signal-to-background ratio, specific labeling, large imaging depth and the like, is applied to scenes such as blood vessel imaging, tumor/inflammation labeling, cavity organ radiography and the like, and can provide a convenient observation means for surgical navigation. In macro imaging, it is generally necessary to use excitation light and a beam expander to form an excitation light path to provide a large excitation light power density, and to use a lens, a filter, a camera, or the like to form an imaging light path.
The near infrared region is a wave band with the wavelength of 900-1880nm, and the near infrared region light has lower scattering in tissues relative to visible light. And, selecting a wave band with larger tissue absorption (such as around 1450 nm) for fluorescence detection helps to further improve the proportion of ballistic light in a fluorescence signal, and is beneficial to clear imaging of deep tissues. In addition, the selection of a long detection wavelength can reduce the autofluorescence signal of the organism, which is helpful for obtaining a good signal-to-background ratio.
Disclosure of Invention
The invention provides a surgical navigation system for simultaneously providing visible light illumination and exciting a fluorescent probe based on a visible light shadowless lamp, aiming at the problem that an excitation light source of the visible light shadowless lamp and the near-infrared fluorescent probe is required in the current near-infrared fluorescent surgical navigation. The invention relates to a near-infrared fluorescence operation navigation system which is built on the basis of an LED shadowless lamp, a high-brightness near-infrared fluorescence probe and a near-infrared macroscopic imaging system. By using the LED shadowless lamp, visible light is provided for an operator, meanwhile, the fluorescent signal is prevented from being submerged by near-infrared band background light, the shadowless lamp is excited to emit fluorescence by using a high-brightness near-infrared fluorescent probe, a high-signal background ratio fluorescent image is obtained by using a high-sensitivity and low-noise near-infrared camera, and the near-infrared fluorescent image is displayed in real time by using a display device, so that surgical navigation is realized.
The purpose of the invention is realized by the following technical scheme: a visible light illumination and near infrared fluorescence surgical navigation system based on a shadowless lamp comprises the shadowless lamp, an operating table, a fluorescence probe, a lens, a visible light cut-off filter, a camera, a display and a computer.
The visible light emitted by the shadowless lamp is used for illumination when an operator observes by naked eyes, the fluorescent probe is excited at the same time, and then the fluorescent image is collected by the lens and the camera. The near-infrared surgery is navigated by highlighting the fluorescently labeled tissue.
The shadowless lamp and the lens face the operating table, a fluorescent probe is injected into an operating object on the operating table, fluorescence of the fluorescent probe is received by the camera through the lens and converted into an electric signal, and the electric signal is transmitted to the display device for displaying after being processed by the computer;
the fluorescent probe is a near-infrared fluorescent probe;
the lens is a near-infrared anti-reflection lens;
the camera is a near-infrared responsive camera;
the camera filters out visible light by using a visible light cut-off filter before receiving the fluorescence;
the shadowless lamp uses the LED lamp bead as a light-emitting device.
Preferably, fluorescent probes with high absorption, high quantum yield and large Stokes shift, such as quantum dots with strong brightness above 1300nm, are used as the fluorescent probes, so that the background ratio of the fluorescent signals is higher, and the fluorescent signals can be more easily highlighted from the background.
Preferably, a 700nm short-pass infrared cut-off filter is added outside the shadowless lamp LED lamp bead, and only visible light is transmitted, so that the possible near-infrared component of the light source is reduced.
Preferably, the camera uses multi-stage semiconductor refrigeration piece refrigeration or liquid nitrogen refrigeration.
Preferably, the camera uses an indium gallium arsenide InGaAs detector as a photosensitive element; a1300 nm long-pass filter is selected before the detector, and a filter with a large extinction coefficient for light with a wave band less than 1300nm is selected, so that visible light reaching a detection surface is filtered as much as possible, and the autofluorescence influence of tissues such as hair of an organism is reduced.
Preferably, the display device is a display, and displays the near-infrared light fluorescence image.
Preferably, the display device is a projector, visible light marker light is projected to the fluorescent region, and the operator observes the probe labeling result with naked eyes.
Preferably, the camera and the lens can be used in two sets, one set is used for imaging visible light, the other set is used for imaging near infrared light, and the visible light and the near infrared fluorescence image are fused for navigation operation of an operator. The visible light imaging result is fused with the near-infrared fluorescence image, so that the marking area is highlighted, the details provided by the visible light energy are reserved, and the operation of an operator is facilitated.
The invention has the beneficial effects that:
1. the system realizes visible light and near infrared fluorescence dual-channel operation navigation, and is beneficial for operators to distinguish and distinguish the marked region;
2. the near-infrared band fluorescent signal is used, and the near-infrared light has good tissue penetration capability, so that the marked region hidden under the tissue can be clearly shown;
3. a high-power excitation light source is not needed, so that the safety to human eyes is realized;
4. an additional exciting light device is not needed, so that the system is simple in structure and convenient to use, and the shielding for observation and operation of an operator is reduced;
5. compared with the traditional halogen lamp bulb shadowless lamp, the cold light source is used, the influence on the heat radiation of the head of an operator is small, and the experience of the operator is improved;
6. compared with the traditional halogen lamp bulb shadowless lamp which emits near infrared light which cannot be responded by human eyes, the LED with high energy efficiency is used, so that energy is wasted, and electricity is saved;
7. the cost of an additional excitation light source is saved, a commercial shadowless lamp can be directly utilized, and the research and development cost is saved.
Drawings
Fig. 1 is a schematic structural diagram of an imaging system according to the present invention.
Fig. 2 and 3 are graphs of the imaging effect obtained by the experiment.
Detailed Description
The following describes embodiments of the present invention in further detail with reference to the accompanying drawings.
As shown in fig. 1, the visible light illumination and near infrared fluorescence surgical navigation system using a shadowless lamp provided by the invention comprises an LED shadowless lamp, an infrared cut-off filter for the LED, an operating table, a fluorescence probe, a near infrared lens, a visible light cut-off filter, a near infrared camera, a display device, a computer and the like.
The LED shadowless lamp used by the system is based on a commercial shadowless lamp, the LED is used as the lamp bead, the shadow can be reduced, the shadow is not obvious (the part which is particularly dark in the shadow is called the shadow), and possible near infrared light is further filtered out through the infrared cut-off filter. The shadowless lamp is provided with a multi-dimensional adjusting frame, a larger space is arranged below the shadowless lamp for placing an imaging object, the shadowless lamp can translate and rotate in multiple dimensions to illuminate an object, and an operator can observe the objects with different depths and low contrast in an incision and a body cavity optimally. The operation object can be placed on the operation table to receive shadowless lamp illumination, fluorescence imaging and operation. The surgical object is marked with a fluorescent probe in the target area.
The fluorescent probe emits near infrared light after being excited by visible light emitted by the shadowless lamp, the near infrared light is collected by the near infrared lens, the fluorescent light forms an image on a detection surface of the near infrared camera through the visible light cut-off filter, the near infrared camera transmits a picture to the computer for image processing, and a result is displayed on the display device. The camera uses a multi-stage semiconductor refrigeration piece for refrigeration or liquid nitrogen refrigeration; the camera uses an indium gallium arsenide InGaAs detector as a photosensitive element. The display device is a display or a projector, and when the display device is a display, the near infrared fluorescence image is displayed; when the display device is a projector, visible light marker light is projected to the fluorescent region, and an operator can observe the probe marking result with naked eyes.
The camera and the lens can be used in two sets, one set is used for imaging visible light, the other set is used for imaging near infrared light, and navigation operation is carried out on an operator after the visible light and the near infrared fluorescence image are fused.
Example (b): as shown in fig. 1, firstly, an LED shadowless lamp 1 is used, light emitted by a lamp bead of the shadowless lamp passes through a near-infrared cut-off filter 2, and visible light illumination is performed on an operation object 4 placed on an operation table 3, so that an operator can conveniently observe the operation object. After the operation object is marked by the fluorescent probe, the fluorescent probe is excited by visible light emitted by the shadowless lamp to emit near-infrared fluorescence, the near-infrared fluorescence is collected by the near-infrared lens 5 and passes through the visible light cut-off filter 6, the fluorescence is imaged on a detection surface of the near-infrared camera 7, the near-infrared camera transmits a picture to the computer 8 for image processing, and a result is displayed on the display 9 in real time. The operator can observe the fluorescence through the display screen without surgical exposure of the fluorescence-labeled tissue.
Wherein the shadowless lamp is a 10-hole wall type shadowless lamp of Qufukangtai. Quantum dots oily quantum dots (customized products) with a fluorescence peak at 1450nm, manufactured by star violet (shanghai) new material technology development ltd, were used. The quantum dots are modified to be water-soluble by SH-PEG2000, and heavy water with low absorption at the wavelength is selected as a solvent because water has high absorption at the wavelength of 1450 nm. The camera used NIRvana-LN from Princeton Instruments. The near-infrared lens is a natural photoelectric fixed-focus lens with the thickness of 100 mm. A700 nm short-pass filter (FESH700) from Sorbo opto-electronic technologies, Inc. was used to filter out the near infrared components that are likely to be emitted by the LED, and a 1300nm long-pass filter (FELH1300) was used to purify the fluorescence signal. The subject was a mouse, and the operator used an indwelling needle to instill a quantum dot heavy aqueous solution (1mg/mL) retrograde into the mouse bladder through the urethra. The bladder was imaged without opening the abdomen.
Fig. 2 is a near-infrared fluorescence image obtained by excitation of a single visible light LED bead of the shadowless lamp, with an integration time of 30 ms. The upper, central, circular bright spot in the figure is a contrast of the fluorescent dye in the bladder. Therefore, the system can obtain a high-quality near infrared fluorescence image with strong signal and low background of the marked region in the tissue.
Fig. 3 is a bright field image obtained by illumination with a fluorescent lamp containing a near infrared component, and it can be seen that the upper middle circular spot in fig. 2 is in the bladder position.
In conclusion, the invention provides a system for simultaneously realizing visible light illumination and near-infrared fluorescence imaging based on a commercial LED shadowless lamp. The system uses a white light LED to provide visible light illumination for the operator, and simultaneously excites fluorescent materials to generate near-infrared fluorescence for navigation surgery. Due to the use of high-brightness fluorescent materials and a high-sensitivity camera, the system avoids the use of an additional light source as excitation light like other near-infrared fluorescence surgery navigation systems, avoids possible damage to the eyes, simplifies the structure of illumination and reduces the cost. The system has reliable performance and simple use, and has wide prospect in near-infrared fluorescence navigation operation.
The above-described embodiments are intended to illustrate rather than to limit the invention, and any modifications and variations of the present invention are within the spirit of the invention and the scope of the appended claims.
Claims (10)
1. A visible light illumination and near infrared fluorescence surgical navigation system based on a shadowless lamp is characterized by comprising the shadowless lamp, an operating table, a fluorescence probe, a lens, a camera, a display device and a computer;
the visible light emitted by the shadowless lamp provides illumination for an operator to observe by naked eyes, and simultaneously excites the fluorescent probe; the shadowless lamp and the lens face the operating table, a fluorescent probe is injected into an operating object on the operating table, fluorescence of the fluorescent probe is received by the camera through the lens and converted into an electric signal, and the electric signal is transmitted to the display device for displaying after being processed by the computer;
the fluorescent probe is a near-infrared fluorescent probe;
the lens is a near-infrared anti-reflection lens;
the camera is a near-infrared responsive camera;
the camera filters out visible light by using a visible light cut-off filter before receiving the fluorescence;
the shadowless lamp uses the LED lamp bead as a light-emitting device.
2. The shadowless lamp-based visible light illumination and near infrared fluorescence surgical guidance system of claim 1, wherein the fluorescent probe is selected from quantum dots with a peak of luminescence above 1300 nm.
3. The shadowless lamp-based visible light illumination and near infrared fluorescence surgical navigation system of claim 1, wherein the camera uses multi-stage semiconductor chilling plate refrigeration or liquid nitrogen refrigeration.
4. The shadowless lamp based visible light illumination and near infrared fluorescence surgical navigation system of claim 1, wherein the camera uses an indium gallium arsenide InGaAs detector as a light sensing element.
5. The shadowless lamp based visible light illumination and near infrared fluorescence surgical navigation system of claim 1, wherein the shadowless lamp uses a near infrared cut filter to filter out possible near infrared components in the light source.
6. The shadowless lamp-based visible light illumination and near infrared fluorescence surgical navigation system of claim 5, wherein the near infrared cut-off filter is a 700nm short pass filter.
7. The system for visible light illumination and near-infrared fluorescence surgical navigation based on shadowless lamps as claimed in claim 1, wherein the visible light cut-off filter is a 1300nm long pass filter.
8. The shadowless lamp-based visible light illumination and near infrared fluorescence surgical navigation system of claim 1, wherein the display device is a display displaying a near infrared fluorescence image.
9. The shadowless lamp-based visible light illumination and near infrared fluorescence surgical navigation system of claim 1, wherein the display device is a projector, visible marker light is projected to a fluorescent area, and probe marking results are observed by an operator using naked eyes.
10. The shadowless lamp-based visible light illumination and near infrared fluorescence surgery navigation system as claimed in claim 1, wherein two sets of the camera and the lens can be used, one set is used for imaging visible light, the other set is used for imaging near infrared light, and the visible light and the near infrared fluorescence image are fused for navigation surgery of a surgeon.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117838065A (en) * | 2024-03-07 | 2024-04-09 | 江苏百宁盈创医疗科技有限公司 | Method, device, apparatus and storage medium for detecting self-fluorescence tissue |
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Application publication date: 20220408 |