CN114287625A

CN114287625A - Carotenoid preparation and application thereof

Info

Publication number: CN114287625A
Application number: CN202111682238.3A
Authority: CN
Inventors: 李倩; 李晓贺; 耿佳鑫; 陈剑彬; 范超; 吴文忠
Original assignee: Innobio Corp ltd
Current assignee: Innobio Corp ltd
Priority date: 2021-12-28
Filing date: 2021-12-28
Publication date: 2022-04-08
Anticipated expiration: 2041-12-28
Also published as: CN114287625B

Abstract

The invention discloses a carotenoid preparation and application thereof, the preparation is prepared by mixing pretreated carotenoid and pretreated gelation wall material, and obtaining powder or granule product through emulsification treatment and granulation; wherein the preparation method of the pretreated carotenoid comprises the following steps: mixing carotenoid and 3-5 times of 50-70% ethanol water solution, stirring at 40-50 deg.C for at least 20min, high-speed shearing for dispersing, adding antioxidant, and removing solvent at 70-80 deg.C until ethanol residue is less than 10ppm, and crystal water content is 10-30%. The product produced by the method can reduce pigment dissolution, and can be slowly released, stably and effectively maintain biological activity. Can be widely applied to the fields of food, beverage, health care products, medicines and the like.

Description

Carotenoid preparation and application thereof

Technical Field

The invention relates to the technical field of particle preparation, and particularly relates to a carotenoid preparation and application thereof.

Background

Carotenoids (carotenoids) are yellow, orange-red or red polyenes, generally composed of 8 isoprenoid units, of the formula C₄₀H₅₆. Carotenoids are known to have beneficial effects on health, and are physiological antioxidants that block lipid peroxidation and thus protect the follicular and uterine steroidogenic cells from oxidation. The carotenoid is provitamin A, and has effects of preventing nyctalopia, resisting oxidation, preventing cancer, and improving tinting strength. However, none of the carotenoids are soluble in water, whereasThe solubility in fats and oils is low and at the same time unstable to light, oxygen and heat, limiting their use. Conventional methods improve the bioavailability and also the coloring power of encapsulated products formed by microencapsulation of carotenoids, for example, some beautiful cakes and beverages are derived from carotenoids.

Meanwhile, the improvement of the coloring capability brings troubles, for example, the product is easily dyed on hands, clothes and even faces, and after the carotenoid product is taken, the surface of the tongue and a cup are also dyed, so that a bad experience is brought to people. None of the methods can maintain the biological activity of the carotenoid, reduce the dyeing ability of the carotenoid, and the like, so that the carotenoid can be better used by people, and is a problem which is considered by researchers in the field. A common solution to this problem is to crosslink the gelatin or protein to give crosslinked beads.

For example, patent GB993138 discloses a method for stabilizing gelatin-containing vitamin products, in which formaldehyde, glutaraldehyde, or the like is used as a crosslinking agent, and heat treatment is performed.

Patent CN1283454A, provides a method for preparing beads of fat-soluble substances by crosslinking gelatin using radiation or glutaminase, followed by spray drying into starchy powder.

Patent CN1764439A, provides a method for preparing crosslinked beads, which are crosslinked at 90-140 ℃ for 30 seconds-30 minutes.

The main problem of these patents is that high temperatures are used for crosslinking and the crosslinking time is too long, thus destroying the fat-soluble ingredients or carotenoids which are not stable.

Patent CN1303670A provides a powdered stable vitamin and/or carotenoid product and a preparation method thereof, which uses an alkali metal phosphate cross-linking agent to cross-link protein materials such as gelatin, casein and the like, and achieves the effect of insolubilization in water at 100 ℃ within 3 min. Although it is claimed to lower the thermal crosslinking temperature, the actual preparation process still uses the condition that the heating temperature is 55-180 ℃.

The above patent solves or partially solves the problem of water solubility of carotenoids, but uses gelatin and protein, which are obviously crosslinked to partially achieve the purpose of reducing pigment dissolution. However, there is no teaching in the prior art how to prepare a product of a non-staining carotenoid preparation using other kinds of wall materials.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides a carotenoid preparation prepared by using non-gelatin and protein raw materials as wall materials and application thereof, which can effectively reduce the coloring capability of the carotenoid and simultaneously still maintain the biological activity of the carotenoid application.

The invention provides a carotenoid preparation, which is prepared by mixing pretreated carotenoid and pretreated gelation wall material, emulsifying and granulating to obtain powder or granule product;

wherein the preparation method of the pretreated carotenoid comprises the following steps: mixing carotenoid and 3-5 times of 50-70% ethanol water solution, stirring at 40-50 deg.C for at least 20min, high-speed shearing for dispersing, adding antioxidant, and removing solvent at 70-80 deg.C until ethanol residue is less than 10ppm, and crystal water content is 10-30%.

With respect to the above-mentioned technical solutions, preferably, the carotenoid is selected from lutein and its fatty acid ester, zeaxanthin, lycopene, alpha-carotene, beta-carotene, canthaxanthin, astaxanthin or mixtures thereof.

The term "a mixture" or "a mixture thereof" as used herein means a mixture of any two or more of the aforementioned components in any ratio, unless otherwise specified.

For the above technical solution, preferably, the total pigment content of the pretreated carotenoid is more than 80%; more preferably selected from lutein or fatty acid esters thereof, zeaxanthin, beta-carotene or mixtures thereof; most preferred is a mixture of lutein or its fatty acid ester, zeaxanthin, beta-carotene in a weight ratio of (1-3) to (1-3).

For the above-described embodiments, it is preferred that the zeaxanthin contains two isomers, namely (3R,3 'R) -zeaxanthin and (3R, 3' S) -zeaxanthin; wherein the proportion of (3R,3 'R) -zeaxanthin and (3R, 3' S) -zeaxanthin is 5% -15%: 95 to 85 percent.

For the above technical solution, preferably, the preparation method of the pretreated gelling wall material is: mixing the wall material and the carbohydrate according to the weight ratio of 1-5:1, preparing into an aqueous solution with solid content of 50-70%, stirring and dispersing at 50-70 ℃, then stirring for 35-50min at 80-90 ℃, cooling to 60-65 ℃, treating for 100-150min, preferably for 110-130 min.

For the above technical solutions, preferably, the weight ratio of the wall material and the carbohydrate is 1-3: 1; the most preferred weight ratio is 3: 1.

With respect to the above technical solution, preferably, the wall material is at least one selected from starch sodium octenyl succinate, acacia and cellulose derivatives; most preferred is sodium starch octenyl succinate.

For the above technical solution, preferably, the carbohydrate is selected from monosaccharides having no more than 10 carbons, which are linked by glycosidic bonds to form straight or branched chain sugars, such as but not limited to sucrose, glucose syrup, xylose, malto-oligosaccharide, fructo-oligosaccharide, solid corn syrup or a mixture thereof. Among them, glucose syrup having a solid content of 50 to 90%, and sucrose or a mixture thereof in any ratio are preferable.

For the above technical solutions, preferably, the granulation method includes spray drying, fluidized drying, etc. to obtain powder or granule product.

For the above-described solution, preferably the amount of gelling wall material is calculated based on the mass of the final carotenoid preparation product, which is 40-70%, preferably 50-70% of the mass of the final product.

For the above technical solution, preferably, the antioxidant is selected from ascorbic acid, ascorbyl palmitate, sucrose fatty acid ester, tocopherol, fatty acid ascorbate, Butyl Hydroxy Toluene (BHT), Butyl Hydroxy Anisole (BHA), propyl gallic acid, tert-butyl hydroxy quinoline or a mixture thereof; more preferably: ascorbic acid, ascorbyl palmitate and sucrose fatty acid ester are mixed according to the weight ratio of 2-5: 0.1-3: 0.1-3 mixing.

For the above-mentioned technical solutions, it is preferred that the antioxidant is used in an amount of 5-30% by weight of the carotenoid. More preferably 18-28%.

With respect to the above technical solutions, it is preferable that the carotenoid preparation has a dissolution rate of the pigment in water of less than 5%.

With respect to the above-mentioned technical solutions, preferably, the carotenoid preparation has a sustained release effect, and the release rate in gastrointestinal fluids is 0.5h < 35%, 4h > 90%.

The invention provides the application of the non-staining carotenoid preparation, which comprises the application in the fields of food, drinks, health products, medicines and the like; especially in products for realizing the visual effect of nutrients in soft sweets, solid beverages and liquid beverages, and can also be applied to eye gel and eye drops.

Compared with the prior art, the invention has the following beneficial effects:

1. after the product is treated by the method, the product has the characteristic of reducing pigment dissolution, and is particularly suitable for products requiring a nutrient visualization effect.

2. After the treatment by the method, the product has the characteristic of slow release, and the product is better preserved in the stomach and achieves the acid resistance.

3. After the carotenoid is pretreated, the stability is improved, and the biological activity of the carotenoid can be still maintained.

4. Organic solvent is not used in the operation process, and the preparation process is green and environment-friendly.

5. Non-gelatin and protein raw materials are used as wall materials, so that the method for preparing the non-dyeing carotenoid preparation is enriched.

Drawings

FIG. 1 is a graph of the products prepared by the methods of example 2 and comparative example 4 according to the present invention applied to soft sweets, showing that a is the appearance of transparent sweets not impregnated with carotenoids prepared by the method of the present invention, and B is the appearance of transparent sweets colored red with respect to beta-carotene product B prepared by the method of comparative example 4, and the transparency of the sweets is reduced.

Detailed Description

The present invention is further illustrated by the following examples, but it should be understood that the scope of the present invention is not limited by the examples.

In the present invention, percentages and percentages are by mass unless otherwise specifically indicated. Unless otherwise specified, the experimental methods used are conventional methods, and the materials, reagents and the like used are commercially available.

The non-dyeing carotenoid preparation can be selectively added with one or more of the following components according to the conventional dosage in the field:

(1) water-soluble ingredients such as, but not limited to, glucose, lactose, malto-oligosaccharide, polyethylene glycol, sodium carboxymethylcellulose, and solid glucose syrup.

(2) Surfactants, to solve the problem of the water-insoluble fat or waxy material that the product usually floats on the water surface, increase the water dispersibility of the product. The surfactant can be exemplified by, but not limited to, tween 60, tween 80 or sucrose fatty acid ester.

(3) Binders, such as but not limited to povidone, glycerin, propylene glycol, polyglycerin fatty acid esters, soluble soybean polysaccharides, sodium carboxymethylcellulose.

(4) Suspending agents, such as but not limited to guar gum, xanthan gum, sodium alginate, hydroxypropyl methylcellulose, gellan gum, carrageenan, may be mentioned.

(5) Diluents, such as but not limited to starch, maltodextrin, calcium hydrogen phosphate.

(6) Examples of stabilizers include, but are not limited to, sodium lactate, sodium citrate, magnesium carbonate, sodium bicarbonate, microcrystalline cellulose.

(7) Glidants such as, but not limited to, silicon dioxide, corn starch, calcium silicate may be mentioned.

(8) Antioxidants, such as but not limited to vitamin E, vitamin C and derivatives thereof, may be mentioned.

(9) Examples of the acidity regulator include, but are not limited to, citric acid, lactic acid, and malic acid.

The following methods were used in the present invention to measure and evaluate the products.

The method for measuring the dissolution rate of the pigment comprises the following steps: and adding 50mL of water into 1g of the product, stirring and dissolving for 30min at 90 ℃ and 100 rpm, filtering and transferring into a volumetric flask, washing with 30mL of water once, combining filtrates, and determining the OD value of the maximum absorption wavelength after constant volume. The dissolution rate of the pigment is (OD value/product quality) multiplied by 100%.

The product accelerated stability evaluation method provided by the invention is a method provided by Chinese pharmacopoeia: the pigment content at different times is measured under the conditions of 40 ℃ and 75% RH to determine the stability, and the product stability is expressed by the pigment retention rate. Pigment retention is the ratio of product content to initial content at different times, expressed as a percentage.

The RELEASE rate test according to the present invention was carried out according to DELAYD-RELEASE DOSAGE FORMS method B using Apparatus 2 at a rotation speed of 50rpm with reference to DISSOLUTIO of USP < 711 > in which 0.1N hydrochloric acid solution was selected as the RELEASE medium for two hours and phosphate buffer solution of pH6.8 was selected as the RELEASE medium for 2 to 8 hours.

EXAMPLE 1 Effect of the method of treating the crystals on the stability of the crystals

(1) 160g of zeaxanthin crystals and 4 times of 60% ethanol aqueous solution are mixed, stirred at 45 ℃ for 30min, then subjected to high-speed shearing dispersion at 10000 revolutions, added with 28g of ascorbic acid, 10g of ascorbyl palmitate and 10g of sucrose fatty acid ester, subjected to solvent removal at 75 ℃, and subjected to ethanol dissolution residue of 8ppm, wherein the water content of the crystals is 12.3%, and the zeaxanthin crystals A are obtained.

(2) 160g of zeaxanthin crystals and 4 times of 60% ethanol aqueous solution are mixed, stirred at 45 ℃ for 30min, then subjected to high-speed shearing dispersion at 10000 revolutions, and added with 28g of ascorbic acid and 10g of ascorbyl palmitate, the solvent is removed at 75 ℃, the ethanol residue is 55ppm, the water content of the crystals is 25%, and zeaxanthin crystals B are obtained.

(3) 160g of zeaxanthin crystals and 8 times of 80% ethanol aqueous solution are mixed, stirred at 45 ℃ for 30min, then subjected to high-speed shearing dispersion at 10000 revolutions, and then 28g of ascorbic acid, 10g of ascorbyl palmitate and 10g of sucrose fatty acid ester are added, solvent is removed at 75 ℃, ethanol residue is 155ppm, and the water content of the crystals is 32%, so that zeaxanthin crystals C are obtained.

(4) The zeaxanthin crystal D is obtained by mixing 160g of zeaxanthin crystals, 28g of ascorbic acid, 10g of ascorbyl palmitate and 10g of sucrose fatty acid ester.

(5) The zeaxanthin crystals a-D were heated at 60 ℃ and the pigment retention was measured at different times, with the results as given in table 1 below:

TABLE 1 pigment Retention at different times

	A	B	C	D
					0	100％	100％	100％	100％
1h	95.4％	88.2％	60.1％	55.2％
					2h	93.1％	65.2％	55.0％	40.4％
4h	90.2％	55.0％	35.0％	28.1％

In addition, zeaxanthin with different isomer ratios was selected for comparative experiments, i.e.: the zeaxanthin contains two isomers of (3R,3 'R) -zeaxanthin and (3R, 3' S) -zeaxanthin, and the weight ratio of the two isomers to the zeaxanthin is more than 80%; and the weight ratio between the two isomers (3R,3 'R) -zeaxanthin and (3R, 3' S) -zeaxanthin is 5-15%: when the content is in the range of 95-85%, the result of the step (1) is not affected.

Example 2

Mixing 200g of lutein crystal with 3 times of 60% ethanol aqueous solution, stirring at 45 ℃ for 30min, performing high-speed shearing dispersion at 10000 revolutions, adding 20g of ascorbic acid, 1g of ascorbyl palmitate and 1g of sucrose fatty acid ester, removing solvent at 75 ℃, dissolving ethanol residue by 7ppm, ensuring that the water content of the crystal is 10%, and freezing at-20 ℃ for later use. 583g of sodium octenyl succinate starch and 195g of glucose are prepared into an aqueous solution with solid content of 50 percent, and after stirring and dispersing at 60 ℃, the temperature is raised to 90 ℃, and stirring is carried out at constant speed for 35 min. Cooling to 60 deg.C, and stirring for 100 min. Mixing the gelatinized wall material and the treated xanthophyll crystal, stirring, emulsifying, and spray drying. The lutein product A is obtained, the pigment dissolution rate is 3.1%, and the release rates of 30min and 4h are 30.5% and 103.3%. The release rates at different times are shown in table 2 below:

TABLE 2 Release degree at different times

Time	Degree of release	Time	Degree of release
				10min	10.0％	3h	101.6％
20min	16.0％	4h	103.3％
				30min	30.5％	6h	101.6％
1h	40.2％	8h	101.6％
				2h	89.4％

Example 3

233g of beta carrot crystals and 5 times of 50% ethanol aqueous solution are mixed, stirred for 40min at 40 ℃, then sheared and dispersed at high speed of 10000 r, 17g of ascorbic acid, 25.5g of ascorbyl palmitate and 25.5g of sucrose fatty acid ester are added, the solvent is removed at 70 ℃, the ethanol solution residue is 5ppm, the water content of the crystals is 15%, and the crystals are frozen for standby at minus 20 ℃. 560g of sodium starch octenylsuccinate and 140g of glucose are prepared into an aqueous solution with 70 percent of solid content, and the aqueous solution is stirred and dispersed at 50 ℃, heated to 80 ℃ and stirred at a constant speed for 50 min. Cooling to 65 deg.C, and stirring for 120 min. Mixing the gelatinized wall material and the treated xanthophyll crystal, stirring, emulsifying, and spray drying. The beta carrot product A is obtained, the pigment dissolution rate is 2.3 percent, and the release rates in 30min and 4h are 28.3 percent and 100.2 percent. The release rates at different times are shown in table 3 below:

TABLE 3 degree of release at different times

Time	Degree of release	Time	Degree of release
				10min	7.9％	3h	101.6％
20min	15.1％	4h	100.2％
				30min	25.3％	6h	101.6％
1h	39.4％	8h	102.2％
				2h	82.9％

Example 4

106g of beta carrot crystal, 212g of lutein ester crystal, 106g of zeaxanthin crystal and 4 times of 70% ethanol aqueous solution are mixed, stirred at 50 ℃ for 55min, then subjected to high-speed shearing dispersion at 10000 revolutions, and added with 73g of ascorbic acid, 1.5g of ascorbyl palmitate and 1.5g of sucrose fatty acid ester, the solvent is removed at 80 ℃, the ethanol residue is 3ppm, the water content of the crystal is 30%, and the crystal is frozen at-20 ℃ for later use. 416.7g of sodium starch octenylsuccinate and 83.3g of glucose syrup are prepared into an aqueous solution with the solid content of 60 percent, and after stirring and dispersing at 70 ℃, the temperature is raised to 85 ℃, and the uniform stirring is carried out for 45 min. Cooling to 63 deg.C, and stirring for 130 min. Mixing the gelatinized wall material and the treated composite crystal, stirring, emulsifying, and spray drying. The lutein ester, zeaxanthin and beta carrot product A with the ratio of 2:1:1 are obtained, the pigment dissolution rate is 3.5%, and the release degrees in 30min and 4h are 22.4% and 99.7%. The release rates at different times are shown in table 4 below:

TABLE 4 degree of release at different times

Time	Degree of release	Time	Degree of release
				10min	10.2％	3h	97.0％
20min	17.2％	4h	99.7％
				30min	28.3％	6h	99.9％
1h	41.4％	8h	99.7％
				2h	79.9％

Comparative example 5

200g of beta carotene crystal and 3 times of 60% ethanol aqueous solution are mixed, stirred for 30min at 45 ℃, then sheared and dispersed at high speed of 10000 r, 20g of ascorbic acid, 1g of ascorbyl palmitate and 1g of sucrose fatty acid ester are added, solvent is removed at 75 ℃, ethanol residue is 7ppm, the water content of the crystal is 12.8%, and the crystal is frozen for standby at-20 ℃. 583g of sodium starch octenylsuccinate and 195g of glucose are prepared into an aqueous solution with the solid content of 50 percent, stirred and dissolved at 60 ℃, added with the beta carotene crystal, stirred, emulsified and spray-dried. The beta carotene product B is obtained, and the pigment dissolution rate is 65.8 percent. The release rates at 30min and 4h were 99.1% and 98.9%.

The contrast shows that the chroma of the product is improved and the slow release effect is deteriorated by the wall material which is not subjected to gelation treatment.

Comparative example 6

200g of lutein ester crystals and 3 times of 60% ethanol aqueous solution are mixed, stirred for 30min at 45 ℃, then subjected to high-speed shearing dispersion at 10000 revolutions, added with 20g of ascorbic acid, 1g of ascorbyl palmitate and 1g of sucrose fatty acid ester, subjected to solvent removal at 75 ℃, subjected to ethanol dissolution residue of 5.3ppm, and frozen at-20 ℃ for later use, wherein the water content of the crystals is 25%. 583g of sodium octenyl succinate starch and 195g of glucose are prepared into an aqueous solution with 50 percent of solid content, and after stirring and dispersing at 60 ℃, the temperature is raised to 100 ℃, and stirring is carried out at a constant speed for 60 min. Mixing the gelatinized wall material and the treated lutein ester crystal, stirring, emulsifying, and spray drying. The lutein ester product A is obtained, and the pigment dissolution rate is 85.2%. The 30min and 4h release rates were 97.7% and 102.3%.

In contrast, the treatment under conditions other than the gelation method improved the color of the product and deteriorated the sustained-release effect.

Comparative example 7

Adding 200g of lutein crystal into 20g of ascorbic acid, 1g of ascorbyl palmitate and 1g of sucrose fatty acid ester, mixing, preparing 583g of starch sodium octenyl succinate and 195g of glucose syrup into a 50% solid content aqueous solution, stirring at 60 ℃, dispersing, heating to 90 ℃, and uniformly stirring for 35 min. Cooling to 60 deg.C, and stirring for 100 min. Mixing the gelatinized wall material and the mixed xanthophyll crystal, stirring, emulsifying, and spray drying. The lutein product B with the pigment dissolution rate of 33.2 percent is obtained. The release rates at 30min and 4h were 90.1% and 95.5%.

As can be seen by comparison, the chroma and the release degree of the product are both deteriorated without the pretreatment of the crystals.

Comparative example 8

Mixing 200g of lutein crystal with 3 times of 60% ethanol aqueous solution, stirring at 45 ℃ for 30min, performing high-speed shearing dispersion at 10000 revolutions, adding 20g of ascorbic acid, 1g of ascorbyl palmitate and 1g of sucrose fatty acid ester, removing solvent at 75 ℃, dissolving ethanol residue by 7ppm, ensuring that the water content of the crystal is 10%, and freezing at-20 ℃ for later use. 583g of corn starch and 195g of glucose are prepared into an aqueous solution with 50 percent of solid content, and after stirring and dispersing at 60 ℃, the temperature is raised to 90 ℃, and stirring is carried out at constant speed for 35 min. Cooling to 60 deg.C, and stirring for 100 min. Mixing the gelatinized wall material and the treated xanthophyll crystal, stirring, emulsifying, and spray drying. The lutein product C is obtained, the pigment dissolution rate is 73.5%, and the release rates in 30min and 4h are 99.5% and 101.3%.

The comparison shows that the effect is poor when sodium starch octenyl succinate is replaced by corn starch.

Effect example 9

Lutein, zeaxanthin and beta-carotene were prepared according to the method of patent 201711456450.1 at a weight ratio of 1:1:1 to obtain composite product 1, and lutein, zeaxanthin and beta-carotene were prepared according to the method of example 2 at a weight ratio of 1:1:1 to obtain composite product 2, and the comparative parameters of the two products are as follows in table 5:

TABLE 5 two product comparison parameters

Effect example 10: evaluation of fondant applications

Weighing 8g of gelatin, 44g of white granulated sugar and 55g of glucose syrup, adding water with 20% of solid matters, stirring for dissolving, decocting at 120 ℃ until the solid matters are about 85%, and adjusting the pH value to 3-4; solution I was obtained.

The lutein product A obtained by the method of example 2 or the beta-carotene product B20g obtained by the method of example 5 are respectively added into the solution I and stirred uniformly. Keeping the temperature at 90 ℃ for 40 min. And (5) injection molding and drying. The appearance is shown in picture 1. It can be seen that the lutein product A has no staining phenomenon in the soft sweets, is completely stored in the soft sweets, and realizes the effect of nutrition visualization. While beta carotene product B stained the fondant red and the transparency of the fondant was reduced.

Effect example 11: evaluation of solid beverage application

The lutein product a obtained by the method of example 2 and the β -carotene product B obtained by the method of example 5 were mixed with appropriate amounts of citric acid 0.03%, maltodextrin 20%, xanthan gum 0.6%, and the like, respectively, to prepare a solid beverage, which was then reconstituted and evaluated. As shown in table 6:

TABLE 6 evaluation of effects

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and those skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A carotenoid preparation is characterized in that a powder or granule product is obtained by mixing pretreated carotenoid and pretreated gelation wall material, emulsifying and granulating;

2. The carotenoid formulation according to claim 1 wherein the carotenoid is selected from lutein and its fatty acid esters, zeaxanthin, lycopene, alpha-carotene, beta-carotene, canthaxanthin, astaxanthin or mixtures thereof.

3. The carotenoid formulation of claim 1 wherein the carotenoid has a total pigment content of greater than 80% after pretreatment.

4. The carotenoid formulation according to claim 1, characterized in that the pre-treated gelatinized wall material is prepared by: mixing the wall material and the carbohydrate according to the weight ratio of 1-5:1, preparing into aqueous solution with solid content of 50-70%, stirring and dispersing at 50-70 ℃, then stirring for 35-50min at 80-90 ℃, cooling to 60-65 ℃, and treating for 100-150 min;

the wall material is selected from at least one of starch, Arabic gum and cellulose derivatives;

the carbohydrate is selected from sucrose, glucose syrup, xylose, malto-oligosaccharide, fructo-oligosaccharide, solid corn syrup or mixture thereof.

5. The carotenoid formulation of claim 4 wherein the wall material is sodium starch octenyl succinate and the carbohydrate is glucose or glucose syrup and combinations thereof.

6. The carotenoid formulation according to claim 4, wherein the weight ratio of wall material to carbohydrate is 1-3: 1.

7. The carotenoid formulation according to claim 1, wherein the amount of the gelling wall material is 40-70% by weight of the final carotenoid formulation.

8. The carotenoid formulation of claim 1 wherein the antioxidant is selected from ascorbic acid, ascorbyl palmitate, sucrose fatty acid esters, tocopherol, fatty acid ascorbate, butyl hydroxytoluene, butyl hydroxyanisole, propyl gallic acid, t-butyl hydroxyquinoline, or mixtures thereof.

9. The carotenoid formulation as claimed in claim 1, wherein the antioxidant is ascorbic acid, ascorbyl palmitate or sucrose fatty acid ester in a weight ratio of 2-5: 0.1-3: 0.1-3, in an amount of 5-30% by weight of the carotenoid.

10. The carotenoid formulation of claim 1, wherein the pigment dissolution of the formulation in water is less than 5%; the release speed of the medicine in gastrointestinal fluid is less than 35% in 0.5h and more than 90% in 4 h.

11. Use of the carotenoid preparation according to claim 1 in the fields of food, beverages, health products, pharmaceuticals and the like.

12. Use according to claim 11, characterized in that: the carotenoid preparation is applied to the preparation of products requiring the visual effect of nutrients, soft sweets, solid beverages and liquid beverages, or the preparation of eye gels and eye drops.