CN108148444B - Preparation method of high-content beta-carotene microcapsule for coloring - Google Patents

Preparation method of high-content beta-carotene microcapsule for coloring Download PDF

Info

Publication number
CN108148444B
CN108148444B CN201711489328.4A CN201711489328A CN108148444B CN 108148444 B CN108148444 B CN 108148444B CN 201711489328 A CN201711489328 A CN 201711489328A CN 108148444 B CN108148444 B CN 108148444B
Authority
CN
China
Prior art keywords
carotene
beta
parts
weight
water
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201711489328.4A
Other languages
Chinese (zh)
Other versions
CN108148444A (en
Inventor
汪志明
李翔宇
余道政
胡雄
陆姝欢
肖敏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cabio Biotech Wuhan Co Ltd
Original Assignee
Cabio Biotech Wuhan Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cabio Biotech Wuhan Co Ltd filed Critical Cabio Biotech Wuhan Co Ltd
Priority to CN201711489328.4A priority Critical patent/CN108148444B/en
Publication of CN108148444A publication Critical patent/CN108148444A/en
Application granted granted Critical
Publication of CN108148444B publication Critical patent/CN108148444B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
    • C09B67/0001—Post-treatment of organic pigments or dyes
    • C09B67/0004—Coated particulate pigments or dyes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/40—Colouring or decolouring of foods
    • A23L5/42—Addition of dyes or pigments, e.g. in combination with optical brighteners
    • A23L5/43—Addition of dyes or pigments, e.g. in combination with optical brighteners using naturally occurring organic dyes or pigments, their artificial duplicates or their derivatives
    • A23L5/44—Addition of dyes or pigments, e.g. in combination with optical brighteners using naturally occurring organic dyes or pigments, their artificial duplicates or their derivatives using carotenoids or xanthophylls
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
    • C09B67/0097—Dye preparations of special physical nature; Tablets, films, extrusion, microcapsules, sheets, pads, bags with dyes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Fodder In General (AREA)

Abstract

The invention provides a preparation method of a high-content beta-carotene microcapsule for coloring, which comprises the following steps: s1, melting a mixture containing 10-30 parts by weight of beta-carotene, 10-20 parts by weight of a first emulsifier and 1-3 parts by weight of an antioxidant to prepare a beta-carotene aqueous solution; step S2, uniformly mixing a mixture containing a second emulsifier and a surfactant to prepare a water-soluble modifier; and step S3, carrying out on-line shearing, homogenizing and vacuum drying on the mixture of the beta-carotene aqueous solution and the water-soluble modifier to obtain the beta-carotene water-soluble modifier. The method for preparing the beta-carotene microcapsule provided by the invention can shorten the preparation time, improve the efficiency, effectively reduce the loss of the beta-carotene and improve the content of the all-trans beta-carotene, has no solvent residue, and the produced high-content beta-carotene microcapsule has good water solubility and no precipitate after being dissolved in water.

Description

Preparation method of high-content beta-carotene microcapsule for coloring
Technical Field
The invention relates to a carotene preparation technology, in particular to a preparation method of a high-content beta-carotene microcapsule for coloring.
Background
Beta-carotene is a fat-soluble compound, is an indispensable nutrient for human bodies, and has remarkable functions in preventing various degenerative diseases caused by aging and senility. Also, beta-carotene is the most ubiquitous and stable natural pigment in nature.
The beta-carotene can be widely used as yellow pigment to replace oil-soluble tar pigment, and can be widely applied to other edible oil, cakes, shortening and the like. For food, oil phase solvent is usually used. For example, chinese patent application publication No. CN102796398A, which discloses a method for preparing β -carotene microcapsules for coloring.
Dispersing 100 parts of dry waxy corn starch by adding water into 30-40% of starch milk, adjusting the pH value to 5.5-6.5, and performing enzymolysis for 10-30 minutes at 75-90 ℃ in the presence of 0.1-1 part of high-temperature resistant alpha-amylase; and then dropwise adding a melt of 1-10 parts of beta-carotene, 1-3 parts of octenyl succinic anhydride and 0-30 parts of vegetable oil into the starch milk at a constant speed within 30-90 minutes under the protection of nitrogen, continuing shearing and emulsifying for 30-60 minutes after dropwise adding, and maintaining the pH value of the system at 8-9.
The method can control byproducts generated along with high-temperature conditions to a certain extent. However, since vegetable oil is still used in the preparation process, and the reaction lasts for a long time under high temperature conditions, the inhibition effect of the oxidation and/or isomerization of β -carotene still needs to be improved.
The beta-carotene is difficult to dissolve in an aqueous solution system, so that the beta-carotene is easily lost in the preparation process. At present, the content of beta-carotene in the beta-carotene microcapsules for coloring is low. The mass content of beta-carotene in the beta-carotene powders used for coloring on the market is usually 1-2%.
In addition, in the conventional microcapsule preparation method, β -carotene is mostly prepared by dissolving β -carotene in an oil phase to form a dispersion system containing β -carotene.
However, various solvents used in the oil phase generate other harmful substances during high-temperature treatment, which affects the quality of the microcapsules. Meanwhile, in the long-time high-temperature treatment process, the beta-carotene dissolved in the oil phase is easily oxidized and isomerized, so that the content of all-trans beta-carotene in the microcapsule is low; the high-content beta-carotene microcapsules on the market are prepared by adopting a spray-starch fluidized bed drying technology, and the water solubility of the produced beta-carotene microcapsules is poor due to the fact that starch is insoluble in water.
Disclosure of Invention
The present invention provides a method for preparing high-content beta-carotene microcapsules for coloring, which overcomes or at least partially solves the above problems, in order to solve the technical problems of large beta-carotene loss, severe beta-carotene oxidation and/or isomerization, and poor water solubility of the high-content beta-carotene microcapsules during the preparation of the beta-carotene microcapsules.
According to one aspect of the present invention, there is provided a method for preparing a beta-carotene microcapsule for coloring, comprising the steps of:
s1, melting a mixture containing 10-30 parts by weight of beta-carotene, 10-20 parts by weight of a first emulsifier and 1-3 parts by weight of an antioxidant to prepare a beta-carotene aqueous solution;
step S2, uniformly mixing a mixture containing a second emulsifier and a surfactant to prepare a water-soluble modifier;
and step S3, mixing the beta-carotene aqueous solution and the water-soluble modifier, and then shearing, homogenizing and vacuum-drying the mixture.
The method of the invention mixes the mixture containing the beta-carotene, the first emulsifier and the antioxidant to prepare the beta-carotene aqueous solution with good dispersibility, which is beneficial to improving the water solubility of the prepared beta-carotene microcapsule.
Furthermore, when preparing the beta-carotene aqueous solution, the antioxidant is added, so that the oxidation speed of the beta-carotene can be effectively slowed down, the isomerization of the beta-carotene is reduced, and the activity of the beta-carotene is enhanced.
Further, the beta-carotene aqueous solution comprises 10-30 parts by weight of beta-carotene, 10-20 parts by weight of a first emulsifier and 1-3 parts by weight of an antioxidant. Specifically, the dosage of the beta-carotene, the first emulsifier and the antioxidant is kept in a reasonable range, which can help the beta-carotene to be better dispersed in an aqueous solution system, and simultaneously, the oxidation probability of the beta-carotene is reduced. It is understood that the units of parts by weight in the present invention may be conventional weight units such as g, kg, etc.
Specifically, the first emulsifier and the antioxidant with certain mass can be added into 40-70 parts by weight of water, and a first mixture can be obtained after shearing and dispersing. Slowly adding beta-carotene into the first mixture, and uniformly stirring to obtain a second mixture.
The second mixture is milled using a milling device such as a sand mill to obtain particles of beta-carotene having a diameter of less than 1 μm. And then, carrying out instantaneous high-temperature melting treatment on the second mixture to obtain the beta-carotene aqueous solution.
Specifically, after the beta-carotene aqueous solution and the water-soluble modifier are mixed uniformly, the obtained mixture is subjected to on-line shearing and high-pressure homogenization, so that the beta-carotene aqueous solution and the water-soluble modifier are mixed uniformly. The mixture is fully and uniformly mixed to obtain emulsion with good dispersibility. And then the emulsion is dried in vacuum to obtain the beta-carotene microcapsule.
In a preferred embodiment, a mixture comprising 15-25 parts by weight of beta-carotene, 15-20 parts by weight of a first emulsifier, 1-3 parts by weight of an antioxidant and 10-15 parts by weight of a carbohydrate is melted to make an aqueous solution of beta-carotene.
In a preferred embodiment, the first emulsifier is sodium caseinate, gum arabic, modified starch, soy protein isolate and/or whey protein; preferably gum arabic and/or modified starch. The antioxidant is ascorbyl palmitate, sodium ascorbate, ascorbic acid, sodium erythorbate and/or vitamin E; sodium ascorbate or sodium erythorbate is preferred.
Specifically, the beta-carotene is mixed into the mixture containing the first emulsifier, so that the beta-carotene can be better dissolved/dispersed into an aqueous solution system, and an emulsion with good dispersibility can be obtained in a subsequent reaction. In addition, Arabic gum or modified starch is a reagent which is good in water solubility and non-toxic, and can enhance the water solubility of the prepared beta-carotene microcapsules.
Specifically, the second mixture contains antioxidants such as sodium ascorbate and the like, and the antioxidants not only have good antioxidant performance, but also have good water solubility, and can form an aqueous solution system with good dispersibility with beta-carotene and the first emulsifier.
In a preferred embodiment, the mixture of step S1 further comprises 10-43 parts by weight of carbohydrate. The carbohydrate is one or more of trehalose, sucrose, solid corn syrup starch sugar and maltodextrin; preferably trehalose and/or sucrose. Specifically, the carbohydrate may preferably be trehalose.
In the actual process of preparing the beta-carotene aqueous solution, in order to better adjust the concentration of the beta-carotene so that the beta-carotene can be better dispersed into the aqueous solution system, carbohydrate can also be added as a filler, so that the beta-carotene is effectively contacted with the carbohydrate, and the stability of the prepared beta-carotene microcapsule is increased.
In a preferred embodiment, a mixture comprising 10 to 20 parts by weight of the second emulsifier, 0.5 to 2 parts by weight of the surfactant and 15 to 43 parts by weight of the carbohydrate is mixed to prepare the water-soluble modifier.
In a preferred embodiment, the second mixture comprising beta-carotene, the first emulsifier and the antioxidant is subjected to a melting process under heat and pressure. The temperature for heating and pressurizing is 150-400 ℃; preferably 170 ℃ and 270 ℃. And the pressure of heating and pressurizing is 1.2-4.0 MPa; preferably 1.5 to 3.0 MPa.
Specifically, when the second mixture is melted under heat and pressure, the beta-carotene can be dissolved/dispersed in the aqueous solution system better and more quickly to form a well-dispersed and homogeneous aqueous solution system.
Specifically, the heating temperature should not be too high during the melting process to avoid accelerating the isomerization of beta-carotene and to increase the content of all-trans beta-carotene in the prepared microcapsule. Namely, under the conditions of heating and pressurizing, the heating temperature is kept in a proper range, and under the action of an antioxidant, the isomerization probability of beta-carotene can be effectively reduced, and the content of all-trans beta-carotene in the prepared microcapsule is increased. In addition, in the temperature range, the beta-carotene can be well dispersed in an aqueous solution system.
Specifically, the pressure to be applied is kept in a suitable range during the melting process, i.e., the β -carotene can be effectively dispersed in the aqueous system. When the pressure is too small, the beta-carotene aqueous solution processed at high temperature is easy to vaporize, which is not beneficial to the later shearing homogenization; when the pressure is too high, the requirement on equipment is high, the cost is high, and the safety production is not facilitated.
Therefore, under the condition of heating and pressurizing, the beta-carotene can be well dispersed in an aqueous solution system, the use of an oil phase solvent can be avoided, the generation of byproducts is reduced, and the prepared microcapsule has higher beta-carotene content and higher relative content of all-trans beta-carotene. And when the second mixture is melted under the heating and pressurizing conditions, the loss amount of the beta-carotene can be effectively reduced, so that the cost can be reduced when the high-content beta-carotene microcapsules are prepared.
When the second mixture is treated under the conditions of heating and pressurizing, because the oil phase solvent is not used, the quality of the microcapsule is not reduced because by-products are generated by the high-temperature reaction of the grease when the second mixture is subjected to the heating, pressurizing and melting treatment.
And under the condition of heating and pressurizing beta-carotene, the emulsifier, the antioxidant, the carbohydrate and water, the beta-carotene can be well dispersed into the aqueous solution system, so that the beta-carotene, the emulsifier, the antioxidant, the carbohydrate and the water can form a good aqueous solution dispersion system, and the content of the beta-carotene in the beta-carotene microcapsule can be effectively increased.
Therefore, the beta-carotene, the emulsifier, the antioxidant, the carbohydrate and the water are melted under the conditions of heating and pressurizing, so that the water solubility of the beta-carotene can be effectively enhanced, and the content of the beta-carotene in the prepared microcapsule and the quality of the microcapsule are improved.
In a preferred embodiment, the second mixture is melt-processed for a time period of 1 to 40 seconds, and the mixture containing the beta-carotene, the first emulsifier and the antioxidant is melt-processed; preferably 3-10 s.
Specifically, the second mixture is subjected to melting treatment under heating and pressurizing conditions, and the melting time is short. That is, under the heating and pressurizing conditions, the beta-carotene can be dissolved/dispersed in the aqueous solution system quickly, and an aqueous solution system with good dispersibility is formed.
Compared with the existing method for preparing the beta-carotene microcapsule by adopting the oil-phase solvent, the beta-carotene can be quickly dissolved/dispersed in an aqueous solution system under the heating and pressurizing conditions of the second mixture, so that the melting time is effectively shortened, and the process treatment process is shortened.
On the basis, the melting time is short, so that the probability of oxidation and/or isomerization of the beta-carotene under high temperature can be further reduced, and the content of the all-trans beta-carotene in the prepared microcapsule is higher.
Therefore, the melting treatment is carried out for a very short time under the heating and pressurizing conditions, that is, the β -carotene can be dissolved/dispersed well in the aqueous solution system. Meanwhile, the time of the beta-carotene in a high-temperature environment is effectively shortened, and the risk of oxidation and/or isomerization is reduced.
In a preferred embodiment, the water-soluble modifier comprises a second emulsifier and a surfactant. The second emulsifier is sodium caseinate, Arabic gum, modified starch, soy protein isolate and whey protein; preferably gum arabic and/or modified starch. The surfactant is one or more of monoglyceride, diglycerol fatty acid ester, tween 80, span 85, glyceryl monostearate and citric acid fatty glyceride; preferred are mono-and diglycerol fatty acid esters.
Specifically, a surfactant is contained in a water-soluble modifier mixed with an aqueous solution of β -carotene, and the dissolution/dispersion effect of β -carotene is further improved after the aqueous solution of β -carotene is mixed with the water-soluble modifier. The second emulsifier and the surfactant are both reagents with good water solubility and can form emulsion with good dispersibility with beta-carotene.
In a preferred embodiment, the water-soluble modifier includes 2 to 30 parts by weight of the second emulsifier and 0.5 to 2.5 parts by weight of the surfactant. Preferably, the water-soluble modifier comprises 10-20 parts by weight of a second emulsifier and 1-2 parts by weight of a surfactant.
Specifically, the quality of the second emulsifier and the surfactant in the water-soluble modifier is kept in a proper range to prepare the water-soluble modifier with proper concentration, and after the water-soluble modifier is mixed with the beta-carotene aqueous solution, an emulsion with better dispersibility can be prepared.
In a preferred embodiment, when actually formulating the water-soluble modifier, the second emulsifier, the surfactant, the carbohydrate and 30 to 60 parts by weight of water are mixed and sheared to obtain the water-soluble modifier with good dispersibility.
The carbohydrate is one or more of trehalose, sucrose, solid corn syrup starch sugar and maltodextrin; preferably trehalose and/or sucrose; further, the carbohydrate may preferably be trehalose.
Specifically, the carbohydrate is trehalose, sucrose, corn syrup solids or maltodextrin. Carbohydrate is added into the water-soluble modifier as a filler, so that the formed emulsion can be effectively filled, and the compactness of the emulsion is enhanced.
In a preferred embodiment, the weight ratio of the aqueous beta-carotene solution to the water-soluble modifier is from 0.5:1 to 2:1, preferably from 0.8:1 to 1.7: 1. The weight ratio of the beta-carotene aqueous solution to the water-soluble modifier is kept in a reasonable range, and the dispersion effect of the beta-carotene can be further enhanced, so that a uniform emulsion system with good dispersibility is formed.
In a preferred embodiment, the mixture of aqueous beta-carotene solution and water-soluble modifier is subjected to in-line shearing and homogenization treatment. Specifically, the homogenization is carried out under the condition that the pressure is 30-100 MPa; preferably under the condition of 40-70 MPa. The shearing and homogenizing temperature is 40-90 ℃; preferably 60 to 80 ℃.
In a preferred embodiment, the beta-carotene microcapsule is obtained by shearing and homogenizing a mixture of a beta-carotene aqueous solution and a water-soluble modifier on line and then drying the mixture in vacuum. The vacuum degree of the vacuum drying is 0-100 Pa, and preferably 10-40 Pa; the temperature of the vacuum drying is 30-80 ℃, and preferably 40-60 ℃.
Particularly, the beta-carotene can be well dissolved/dispersed in an aqueous solution system under the conditions of heating and pressurizing and on the basis of reasonably regulating the content of the beta-carotene and other additives. Therefore, in the subsequent drying process, the treatment can be carried out in a vacuum drying mode.
And in the vacuum drying process, the drying can be carried out at a lower temperature, so that the condition that the beta-carotene is easy to oxidize and/or isomerize under a high-temperature condition can be further avoided, the content of the beta-carotene in the prepared beta-carotene microcapsule and the content of all-trans beta-carotene are further improved, the content of the beta-carotene in the prepared beta-carotene microcapsule can reach more than 10 percent, and the content of the all-trans beta-carotene in the beta-carotene can reach more than 90 percent.
In a preferred embodiment, a method for preparing a beta-carotene microcapsule for coloring, comprising:
step S1, melting a mixture containing 15-25 parts by weight of beta-carotene, 15-20 parts by weight of a first emulsifier, 1-3 parts by weight of an antioxidant and 10-15 parts by weight of carbohydrate to prepare a beta-carotene aqueous solution;
wherein the first emulsifier is one or more of sodium caseinate, Arabic gum, modified starch, soy protein isolate and whey protein, the antioxidant is ascorbyl palmitate, sodium ascorbate, ascorbic acid, sodium erythorbate or vitamin E, and the carbohydrate is trehalose or sucrose;
step S2, uniformly mixing a mixture containing 10-20 parts by weight of second emulsifier, 0.5-2 parts by weight of surfactant and 15-43 parts by weight of carbohydrate to prepare a water-soluble modifier; wherein the second emulsifier is Arabic gum and/or modified starch, and the surfactant is mono-diglycerol fatty acid ester or diglycerol fatty acid ester;
and step S3, mixing the beta-carotene aqueous solution and the water-soluble modifier, and then shearing, homogenizing and vacuum-drying the mixture.
In a preferred embodiment, a method for preparing a beta-carotene microcapsule for coloring, comprising:
step S1, melting 10-30 parts by weight of beta-carotene, 10-20 parts by weight of first emulsifier, 1-3 parts by weight of antioxidant and 10-45 parts by weight of carbohydrate for 3-5S under the conditions of 40MPa, 170 ℃ and 270 ℃ to prepare beta-carotene aqueous solution;
wherein the first emulsifier is acacia, the antioxidant is sodium erythorbate, and the carbohydrate is trehalose;
step S2, uniformly mixing a mixture containing 2-30 parts by weight of a second emulsifier, 0.5-2.5 parts by weight of a surfactant and 15-43 parts by weight of carbohydrate to prepare a water-soluble modifier; wherein the second emulsifier is modified starch, and the surfactant is mono-fatty acid glyceride and di-fatty acid glyceride.
And step S3, mixing the beta-carotene water solution with the water-soluble modifier, shearing on line, homogenizing, and then drying in vacuum to obtain the beta-carotene microcapsule.
According to another aspect of the present invention, there is also provided a β -carotene microcapsule prepared by the above-mentioned preparation method.
The beneficial effects of the invention are mainly as follows:
(1) the content of beta-carotene in the prepared beta-carotene microcapsule can reach more than 10 percent, wherein the content of all-trans beta-carotene in the beta-carotene microcapsule can reach more than 90 percent; in the preparation process, the loss rate of the beta-carotene is 1.3 percent;
(2) melting a mixture containing beta-carotene, a first emulsifier and an antioxidant, and enabling the beta-carotene to be better dissolved/dispersed in an aqueous solution system within a reasonable dosage range to form a uniformly dispersed aqueous solution system; the use of an oil phase solvent is avoided, so that the production of byproducts under the high-temperature condition is reduced, and the quality of the prepared beta-carotene microcapsule is effectively improved;
(3) under the condition of heating and pressurizing, the time for carrying out melting treatment on the beta-carotene aqueous solution is short, so that the treatment process is shortened, the beta-carotene can be well dispersed into the aqueous solution system, the loss rate of the beta-carotene is reduced, and the possibility of oxidation and/or isomerization of the beta-carotene can be effectively reduced;
(4) the dosage of the beta-carotene, the first emulsifier, the antioxidant, the second antioxidant and the surfactant is reasonably regulated, the processing time can be further shortened, the possibility of oxidation and/or isomerization of the beta-carotene can be further reduced, and therefore the content of the beta-carotene and the content of all-trans beta-carotene in the prepared beta-carotene microcapsule are increased;
(5) the mixture of the beta-carotene and the water-soluble modifier is dried under low temperature by adopting vacuum drying, so that the isomerization of the beta-carotene under high temperature can be further reduced.
Detailed Description
The following examples are given to further illustrate the embodiments of the present invention. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1
The present embodiment provides a high-content beta-carotene microcapsule for coloring and a preparation method thereof, the method comprising the following three steps:
step 1, preparing a beta-carotene water solution,
adding 20 parts of Arabic gum, 1.5 parts of sodium ascorbate and 12 parts of sucrose into 50 parts of water, shearing and uniformly dispersing to obtain a first mixture, slowly adding 10.5 parts of beta-carotene into the first mixture, and grinding until the average particle size of the beta-carotene is 0.5 mu m to obtain a second mixture; melting the second mixture at 250 ℃ under 2.3MPa for 3s to obtain a beta-carotene aqueous solution;
step 2, preparing a water-soluble modifier,
adding 14 parts of Arabic gum, 2 parts of glyceryl monostearate and 40 parts of starch sugar into 50 parts of water, shearing and mixing for 15min, and uniformly mixing to obtain a water-soluble modifier;
and 3, shearing the beta-carotene aqueous solution and the water-soluble modifier on line, homogenizing under 40MPa, and then drying under vacuum at the vacuum degree of 30Pa and the temperature of 60 ℃ to obtain the beta-carotene microcapsule.
The loss rate of the beta-carotene in the beta-carotene microcapsule prepared by the method is 1.37 percent, and the mass proportion of all-trans beta-carotene in the microcapsule is 92 percent.
Example 2
The present embodiment provides a high-content beta-carotene microcapsule for coloring and a preparation method thereof, the method comprising the following three steps:
step 1, preparing a beta-carotene water solution,
adding 20 parts of Arabic gum, 3 parts of sodium erythorbate and 10 parts of trehalose into 63 parts of water, shearing and uniformly dispersing to obtain a first mixture, slowly adding 30 parts of beta-carotene into the first mixture, and grinding until the particle size of the beta-carotene is 0.5 mu m to obtain a second mixture; melting the second mixture at 2MPa and 210 ℃ for 3s to obtain a beta-carotene aqueous solution;
step 2, preparing a water-soluble modifier,
adding 20 parts of Arabic gum, 2 parts of mono-diglycerol fatty acid ester and 15 parts of starch sugar into 37 parts of water, shearing and mixing for 15min, and uniformly mixing to obtain a water-soluble modifier;
and 3, shearing the beta-carotene aqueous solution and the water-soluble modifier on line, homogenizing under 40MPa, and then drying under vacuum at the vacuum degree of 20 and the temperature of 55 ℃ to obtain the beta-carotene microcapsule.
The loss rate of the beta-carotene in the beta-carotene microcapsule prepared by the method is 1.3 percent, and the mass proportion of the all-trans beta-carotene in the microcapsule is 94 percent.
Example 3
The present embodiment provides a high-content beta-carotene microcapsule for coloring and a preparation method thereof, the method comprising the following three steps:
step 1, preparing a beta-carotene water solution,
adding 15 parts of modified starch, 1 part of ascorbyl palmitate and 14.5 parts of sucrose into 51 parts of water, shearing and uniformly dispersing to obtain a first mixture, slowly adding 20.5 parts of beta-carotene into the first mixture, and grinding until the particle size of the beta-carotene is 0.5 mu m to obtain a second mixture; melting the second mixture at 240 ℃ under 2.3MPa for 3s to obtain a beta-carotene aqueous solution;
step 2, preparing a water-soluble modifier,
adding 15 parts of Arabic gum, 1 part of span 85 and 41 parts of starch sugar into 49 parts of water, shearing and mixing for 15min, and uniformly mixing to obtain a water-soluble modifier;
and 3, shearing the beta-carotene aqueous solution and the water-soluble modifier on line, homogenizing under 40MPa, and then drying under vacuum at the vacuum degree of 30Pa and the temperature of 55 ℃ to obtain the beta-carotene microcapsule.
The loss rate of the beta-carotene in the beta-carotene microcapsule prepared by the method is 1.67 percent, and the total trans-beta-carotene in the microcapsule accounts for 91 percent of the mass of the beta-carotene.
Example 4
The present embodiment provides a high-content beta-carotene microcapsule for coloring and a preparation method thereof, the method comprising the following three steps:
step 1, preparing a beta-carotene water solution,
adding 10 parts of Arabic gum, 10 parts of modified starch, 1 part of ascorbyl palmitate and 14.5 parts of trehalose into 51 parts of water, shearing and uniformly dispersing to obtain a first mixture, slowly adding 15.5 parts of beta-carotene into the first mixture, and grinding until the particle size of the beta-carotene is 0.6 mu m to obtain a second mixture; melting the second mixture at 2.5MPa and 210 ℃ for 5s to obtain a beta-carotene aqueous solution;
step 2, preparing a water-soluble modifier,
adding 4 parts of sodium caseinate, 1 part of fatty acid monoglyceride and diglyceride and 44 parts of starch sugar into 49 parts of water, shearing and mixing for 15min, and uniformly mixing to obtain a water-soluble modifier;
and 3, shearing the beta-carotene aqueous solution and the water-soluble modifier on line, homogenizing under 40MPa, and then drying under vacuum at the vacuum degree of 35Pa and the temperature of 55 ℃ to obtain the beta-carotene microcapsule.
The loss rate of the beta-carotene in the beta-carotene microcapsule prepared by the method is 1.21 percent, and the mass ratio of the all-trans beta-carotene in the microcapsule to the beta-carotene is 95.7 percent.
Comparative example 1
A production method of beta-carotene microcapsules comprises the following steps:
1. 300 parts of modified starch, 5 parts of cane sugar and 5 parts of vitamin C palmitate are dissolved in 500 parts of water and fully dissolved at 70 ℃.
2. 4.5 parts of beta-carotene (content: 96%), 0.1 part of BHT (2, 6-di-tert-butyl-4-methylphenol) and 0.1 part of vitamin E were dissolved in 50 parts of sunflower seed oil and sufficiently dissolved at 160 ℃.
3. According to the water phase: the oil phase was mixed in line at 14:1 (mass ratio) and homogenized at 60MPa for 2 times to obtain an emulsion.
4. Drying the emulsion by a spray dryer at the air inlet temperature of 120-160 ℃ to obtain 1% beta-carotene microcapsule powder.
The trans content of the beta-carotene microcapsule produced by adopting the oil melting method accounts for about 50 percent, the loss rate is 10 to 20 percent, and the content of the beta-carotene is lower.
Comparative example 2
The production method of the high-content beta-carotene microcapsule comprises the following steps:
1.21 parts of beta-carotene crystals, 18 parts of gelatin, 1.5 parts of sodium ascorbate and 9.5 parts of starch sugar are placed in 48 parts of water, the mixture is stirred until the crystals are dispersed and suspended uniformly to obtain beta-carotene suspension, the suspension is circularly ground for 60min by a ball mill, and then a pipeline heater is used for heating the suspension at the temperature of 170 ℃ and 330 ℃ for 13s to obtain the beta-carotene aqueous solution.
2. 12 parts of Arabic gum, 1 part of mono-fatty acid glyceride, 7 parts of cane sugar are placed in 52 parts of water and stirred uniformly to obtain the water-soluble modifier.
3. Shearing the beta-carotene aqueous solution and the water-soluble modifier on line, homogenizing under 40MPa, and drying in a starch fluidized bed to obtain the beta-carotene microcapsule with the content of 20.5 percent.
After the high-content beta-carotene microcapsule is dissolved in water, most of the product is insoluble, and a small part of precipitate is generated, so that the product is not suitable for coloring.
Finally, the method of the present invention is only a preferred embodiment and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (14)

1. A method for preparing high-content beta-carotene microcapsules for coloring, which is characterized by comprising the following steps:
s1, melting a mixture containing 10-30 parts by weight of beta-carotene, 10-20 parts by weight of a first emulsifier and 1-3 parts by weight of an antioxidant to prepare a beta-carotene aqueous solution; the melting is carried out under the conditions of heating and pressurizing, the temperature of the heating and pressurizing is 150-400 ℃, and the pressure of the heating and pressurizing is 1.2-4.0 MPa; the melting time is 1-40 s;
step S2, uniformly mixing a mixture containing a second emulsifier and a surfactant to prepare a water-soluble modifier;
step S3, mixing the beta-carotene water solution and the water-soluble modifier, and then shearing, homogenizing and vacuum drying to obtain the beta-carotene water-soluble modifier;
the mixture described in step S1 further comprises 10-45 parts by weight of carbohydrate.
2. The method of claim 1, wherein the first emulsifier is one or more of sodium caseinate, gum arabic, modified starch, soy protein isolate, and whey protein; the antioxidant is ascorbyl palmitate, sodium ascorbate, ascorbic acid, sodium erythorbate or vitamin E.
3. The method of claim 2, wherein the first emulsifier is gum arabic and/or modified starch; the antioxidant is sodium ascorbate or sodium erythorbate.
4. The method of claim 1, wherein the carbohydrate is one or more of trehalose, sucrose, corn syrup solids, starch sugar, and maltodextrin.
5. The method of claim 4, wherein the carbohydrate is trehalose and/or sucrose.
6. The method of claim 1, wherein the melting is performed under heat and pressure;
the temperature of heating and pressurizing is 170-270 ℃;
the pressure of heating and pressurizing is 1.5-3.0 MPa.
7. The method of claim 6, wherein the time of melting is 3-10 s.
8. The method of claim 1,
the second emulsifier is one or more of sodium caseinate, Arabic gum, modified starch, soy protein isolate and whey protein; the surfactant is one or more of monoglyceride, diglycerol fatty acid ester, span 85, tween 80, glyceryl monostearate and citric acid fatty glyceride.
9. The method of claim 8,
the second emulsifier is Arabic gum and/or modified starch; the surfactant is mono-diglycerol fatty acid ester or diglycerol fatty acid ester.
10. The method of claim 9,
the water-soluble modifier comprises 2-30 parts by weight of a second emulsifier and 0.5-2.5 parts by weight of a surfactant.
11. The method of claim 10,
the water-soluble modifier comprises 10-20 parts by weight of a second emulsifier and 1-2 parts by weight of a surfactant.
12. The method of any one of claims 1-11, wherein the weight ratio of the aqueous beta-carotene solution to the water-soluble modifier is from 0.5:1 to 2: 1.
13. The method of claim 12,
the weight ratio of the beta-carotene aqueous solution to the water-soluble modifier is 0.8:1-1.7: 1.
14. The method of claim 12, comprising the steps of:
s1, melting a mixture containing 15-25 parts by weight of beta-carotene, 15-20 parts by weight of a first emulsifier, 1-3 parts by weight of an antioxidant and 10-15 parts by weight of carbohydrate to prepare a beta-carotene aqueous solution;
the first emulsifier is one or more of sodium caseinate, Arabic gum, modified starch, soy protein isolate and whey protein, the antioxidant is one of ascorbyl palmitate, sodium ascorbate, ascorbic acid, sodium erythorbate or vitamin E, and the carbohydrate is trehalose or sucrose;
step S2, uniformly mixing a mixture containing 10-20 parts by weight of a second emulsifier, 0.5-2 parts by weight of a surfactant and 15-43 parts by weight of carbohydrate to prepare a water-soluble modifier; wherein the second emulsifier is Arabic gum and/or modified starch, and the surfactant is mono-diglycerol fatty acid ester or diglycerol fatty acid ester;
and step S3, mixing the beta-carotene aqueous solution and the water-soluble modifier, and then shearing, homogenizing and vacuum-drying the mixture.
CN201711489328.4A 2017-12-29 2017-12-29 Preparation method of high-content beta-carotene microcapsule for coloring Active CN108148444B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201711489328.4A CN108148444B (en) 2017-12-29 2017-12-29 Preparation method of high-content beta-carotene microcapsule for coloring

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201711489328.4A CN108148444B (en) 2017-12-29 2017-12-29 Preparation method of high-content beta-carotene microcapsule for coloring

Publications (2)

Publication Number Publication Date
CN108148444A CN108148444A (en) 2018-06-12
CN108148444B true CN108148444B (en) 2020-08-11

Family

ID=62460182

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201711489328.4A Active CN108148444B (en) 2017-12-29 2017-12-29 Preparation method of high-content beta-carotene microcapsule for coloring

Country Status (1)

Country Link
CN (1) CN108148444B (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108703260A (en) * 2018-07-16 2018-10-26 嘉必优生物技术（武汉）股份有限公司 A kind of beta carotene bacterium powder and preparation method thereof
CN109452621B (en) * 2018-11-01 2022-04-15 江南大学 pH-sensitive starch-based microcapsule and preparation method thereof
US11077066B2 (en) 2018-11-01 2021-08-03 Jiangnan University PH-sensitive starch-based microcapsule and its preparation method
CN110305497A (en) * 2019-07-12 2019-10-08 天津宏顺科生物科技有限公司 Method for converting fat-soluble natural pigment into water-soluble pigment and protecting fading of water-soluble pigment
CN111838648B (en) * 2020-06-16 2023-04-25 嘉必优生物技术（武汉）股份有限公司 Device and method for producing nano carotenoid emulsion
WO2024028398A1 (en) * 2022-08-05 2024-02-08 Dsm Ip Assets B.V. Solid formulations comprising beta-carotene

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100500652C (en) * 2007-02-12 2009-06-17 浙江大学 Preparing method of high content full cis-beta-carotene formulation
CN101549273B (en) * 2009-03-30 2011-06-15 浙江新和成股份有限公司 Method of preparing nano-dispersed high-all-trans-carotenoid microcapsules
CN102892414B (en) * 2010-05-18 2015-05-06 富士胶片株式会社 Carotenoid-containing composition and production method therefor
CN104719894B (en) * 2015-03-24 2017-07-07 武汉志邦化学技术有限公司 A kind of preparation technology of bata-carotene microcapsules
CN105747216B (en) * 2016-03-03 2018-11-09 广州市食品工业研究所有限公司 A kind of microcapsules Beta Carotene Powder and preparation method thereof
CN107048367A (en) * 2017-04-24 2017-08-18 广州市食品工业研究所有限公司 A kind of natural Beta-carotene microscapsule powder and preparation method thereof

Also Published As

Publication number Publication date
CN108148444A (en) 2018-06-12

Similar Documents

Publication Publication Date Title
CN108148444B (en) Preparation method of high-content beta-carotene microcapsule for coloring
JP7205835B2 (en) Fat-soluble nutrient microcapsules and method for producing the same
US5364563A (en) Powdered aqueous carotenoid dispersions
JP4608034B2 (en) Liquid carotenoid preparations mixable with oil
CN111419821B (en) Preparation method of high all-trans carotenoid microcapsules
US10004670B2 (en) Ready-to-use, stable emulsion
JP3914619B2 (en) Stable emulsions and dry powders of fat-soluble vitamin mixtures, their production, their use and foodstuffs, pharmaceuticals or animal feeds containing them
JP4124821B2 (en) Stable aqueous dispersions of xanthophylls and stable water-dispersible dry powders, their production and use
US11278856B2 (en) Lutein microcapsule formulation and preparation method thereof
CN109418541B (en) Preparation method of hydrophobic fat-soluble vitamin microcapsule
JP2018011598A (en) Extrusion process
JP2011505125A (en) Carotenoid powder composition for beverage coloring
JPH101616A (en) Production of finely dispersed carotenoid or retinoid slurry, the carotenoid or retinoid slurry and coloring of food and feed
EP2403362B1 (en) Powdered compositions of astaxanthin derivatives ii
EP2124615A1 (en) Unctuous compositions
EP2403360B1 (en) Formulation of astaxanthin derivatives and use thereof iii
CN113575945B (en) Preparation method of nanoscale all-trans carotenoid dry powder
CN112515088B (en) Water-dispersible lutein ester preparation and preparation method and application thereof
EP2403364A1 (en) Method for producing solutions of astaxanthin derivatives
CN101754693A (en) The dispersible carotenoid formulation of water
CN113785987A (en) Preparation method of vitamin A microcapsule
CN115349630A (en) Preparation method of water-dispersible lutein microcapsule powder
AU2017300595B2 (en) Propyl gallate-containing vitamin preparations
CN112205613B (en) Preparation method of starch-based emulsion microgel rich in functional factors
CN112107555B (en) Vitamin A acetate microcapsule and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant