CN111838648B - Device and method for producing nano carotenoid emulsion - Google Patents

Device and method for producing nano carotenoid emulsion Download PDF

Info

Publication number
CN111838648B
CN111838648B CN202010550602.XA CN202010550602A CN111838648B CN 111838648 B CN111838648 B CN 111838648B CN 202010550602 A CN202010550602 A CN 202010550602A CN 111838648 B CN111838648 B CN 111838648B
Authority
CN
China
Prior art keywords
carotenoid
emulsion
heat exchange
exchange chamber
carotene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202010550602.XA
Other languages
Chinese (zh)
Other versions
CN111838648A (en
Inventor
汪志明
李翔宇
杨金涛
陆姝欢
唐孝鹏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cabio Biotech Wuhan Co Ltd
Original Assignee
Cabio Biotech Wuhan Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cabio Biotech Wuhan Co Ltd filed Critical Cabio Biotech Wuhan Co Ltd
Priority to CN202010550602.XA priority Critical patent/CN111838648B/en
Publication of CN111838648A publication Critical patent/CN111838648A/en
Application granted granted Critical
Publication of CN111838648B publication Critical patent/CN111838648B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/03—Organic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/10—General methods of cooking foods, e.g. by roasting or frying
    • A23L5/13—General methods of cooking foods, e.g. by roasting or frying using water or steam
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P30/00—Shaping or working of foodstuffs characterised by the process or apparatus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/04—Dispersions; Emulsions
    • A61K8/06—Emulsions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/31—Hydrocarbons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F28—HEAT EXCHANGE IN GENERAL
    • F28B—STEAM OR VAPOUR CONDENSERS
    • F28B9/00—Auxiliary systems, arrangements, or devices
    • F28B9/10—Auxiliary systems, arrangements, or devices for extracting, cooling, and removing non-condensable gases
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Polymers & Plastics (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Food Science & Technology (AREA)
  • Birds (AREA)
  • Nutrition Science (AREA)
  • Epidemiology (AREA)
  • Dispersion Chemistry (AREA)
  • Mechanical Engineering (AREA)
  • General Engineering & Computer Science (AREA)
  • Dermatology (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The invention belongs to the technical field of food additive preparation, and particularly relates to a device and a method for producing nano carotenoid emulsion. The apparatus comprises a heat exchange chamber in which carotenoids in carotenoid emulsions injected therein are heat-treated by water vapor; the homogenizing system is used for homogenizing the carotenoid emulsion after the heat treatment; a vacuum chamber for partially removing water from the homogenized carotenoid emulsion. The device and the method provided by the invention can prepare the nano-level carotenoid emulsion with high content and uniform dispersion, and simultaneously, the heat treatment time of the carotenoid emulsion is short, the influence on the trans-structure of the carotenoid emulsion caused by long-time heating is avoided, and the device and the method are suitable for industrial mass production of the nano-level carotenoid emulsion.

Description

Device and method for producing nano carotenoid emulsion
Technical Field
The invention belongs to the technical field of food additive preparation, and particularly relates to a device and a method for producing nano carotenoid emulsion.
Background
Carotenoids (carotenoids) are a general term for an important class of natural pigments, commonly found in yellow, orange or red pigments of animals, higher plants, fungi, algae, and can be widely used in the food industry, feed industry, medicine and cosmetics industry. Carotenoids are isoprenoid polymers containing 40 carbons, i.e., tetraterpenes. Typical carotenoids are formed from 8 isoprene units joined end to end. The color of carotenoids varies with the number of conjugated double bonds. At present, common carotenoids include beta-carotene, lycopene, astaxanthin, lutein, zeaxanthin, canthaxanthin and the like, and all the carotenoids can be converted into vitamin A in vivo, but due to conjugated double bonds, the carotenoids are easy to cis-trans isomerise to generate a large number of geometric isomers, so that the physiological functions of the carotenoids are affected.
Beta-carotene (C) 40 H 56 ) Is the most widely used carotenoid at present, is an orange fat-soluble compound, also called provitamin A, 60-70% of vitamin A required by human body comes from beta-carotene, and the geometrical isomer is taken as an example to describe the influence of the beta-carotene on physiological functions.
Beta-carotene (C) 40 H 56 ) The trans isomer naturally occurring in the middle has the highest vitamin a activity and is the predominant form of retinoids and carotenoids naturally occurring in foods. The heat treatment converts it to the cis isomer, which results in a loss of vitamin A activity as detailed in Table 1, sources of data in Table 1: food chemistry: third edition/(Mei), philima; wang et al. During the heat treatment, the retinoid and carotenoid molecules were not chemically altered, but they did isomerize to some extent (analysis by high performance liquid HPLC showed)
TABLE 1 vitamin A relative Activity of beta-carotene stereoisomers
Compounds and isomers Vitamin A relative Activity ① Compounds and isomers Vitamin A relative Activity ①
Beta-carotene Alpha-carotene
All-trans 100 All-trans 53
9-cis (New-U) 38 9-cis (New-U) 13
13-cis (New-B) 53 13-cis (New-B) 16
Note that: (1) activity relative to all-trans beta-carotene obtained by rat bioassay.
Necessity of preparing carotenoid emulsions:
1) Enhancing stability of carotenoids
The structure of carotenoids determines that they are very unstable substances, susceptible to degradation by the action of light, oxygen, moisture, heavy metals, oxidants or reducing agents, and that under changing conditions, less active isomers are produced. But can be further dried to prepare capsules after emulsion preparation treatment, so that the adaptability of the capsules to the environment can be greatly improved, and the stability of the capsules is enhanced.
2) Expanding the application range of carotenoid
Most carotenoids have good coloring effect and remarkable biological functions, have a melting point of 176-184 ℃, are insoluble in water, slightly soluble in vegetable oil, and have moderate solubility in aliphatic and aromatic hydrocarbons. The direct use of carotenoids in foods and beverages is greatly limited due to their lipid solubility, and crude carotenoids are currently rarely used directly in foods and feeds and pharmaceuticals because crude crystalline forms of the materials can only be used in lipid soluble binders and only give poor absorption and poor coloration. In order to increase the color yield and to increase the absorbability, the dissolution capacity is changed and the range of application is widened, which requires that the carotenoid crystals are made into a water-soluble emulsion.
In the prior art, the preparation method of carotenoid emulsion is introduced, and because the carotenoid has similar properties, the melting point range is relatively close, and the preparation of beta-carotene emulsion is taken as an example:
in the prior art, a melting method and a solvent method are generally adopted to prepare beta-carotene emulsion with uniform dispersion to improve the water solubility and stability of beta-carotene, and the specific preparation method comprises the following steps:
melting method: after being dispersed in vegetable oil, beta-carotene crystal is dispersed in emulsion containing emulsifier and antioxidant to form stable emulsion to improve its water solubility and stability. The conventional melting heating method is generally indirect heat exchange, the indirect heat exchange has low heat exchange efficiency and long heating time, so that the trans-structure in the beta-carotene is seriously damaged, and the vegetable oil is required to be dispersed to prepare fluid for heat conduction in the dispersing process, so that the effective components of the beta-carotene produced by multiple dilution are not high, and the preparation of the high-content carotenoid emulsion is not facilitated.
Solvent method: dissolving beta-carotene crystal in organic solvent such as tetrahydrofuran, acetone, isopropanol, etc., shearing, homogenizing, dispersing in emulsion containing emulsifying agent and antioxidant to obtain stable emulsion, and heating to remove boiling point of solvent by utilizing the characteristic that boiling point of solvent is lower than that of water in emulsion. Although the emulsion with stable beta-carotene structure and less isomerism can be prepared, the solvent in the emulsion is difficult to remove, so that the solvent residue in the product is greatly out of standard, and the requirement of the food industry can not be met.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a device and a method for producing nano carotenoid emulsion.
Specifically, the invention provides the following technical scheme.
An apparatus for producing nano-scale carotenoid emulsion, comprising a heat exchange chamber in which carotenoids in carotenoid emulsion injected therein are heat-treated by water vapor;
and the homogenizing system is used for homogenizing the carotenoid emulsion after the heat treatment.
Preferably, the device further comprises a feed liquid buffer chamber, wherein the feed liquid buffer chamber is arranged between the heat exchange chamber and the homogenizing system and is used for receiving carotenoid emulsion flowing out of the heat exchange chamber.
Preferably, in the above device, a spray nozzle is disposed in the heat exchange chamber, the spray nozzle includes a plurality of spray outlets, the spray nozzle conical sprays the carotenoid emulsion into the heat exchange chamber through the spray outlets, more preferably, the aperture of any one spray outlet is 0.8-2 mm, the interval between any two adjacent spray outlets is 40-60 mm, and the number of spray outlets can be set between 1-50.
Preferably, in the above device, an evacuation pipe is provided on the heat exchange chamber, and more preferably, a pneumatic valve for controlling opening or closing of a pipeline is provided on the evacuation pipe.
Preferably, the device further comprises a clean steam generator connected with the heat exchange chamber, wherein the clean steam generator is used for converting industrial water steam into clean water steam and then sending the clean water steam into the heat exchange chamber.
Preferably, the apparatus further comprises a vacuum chamber connected to the homogenizing system for partially removing water from the homogenized carotenoid emulsion.
The present invention also provides a method for producing a nano-scale carotenoid emulsion, comprising the steps of:
(1) Adding water vapor into the heat exchange chamber and maintaining the pressure in the heat exchange chamber at 5-15bar;
(2) Spraying carotenoid emulsion with a certain initial mass concentration into the heat exchange chamber to perform heat treatment on the carotenoid, and conveying the carotenoid emulsion after heat treatment into a homogenizing system to be homogenized.
Preferably, in the above method, in step (1), the water vapor is clean saturated water vapor.
Preferably, the method further comprises the following steps: and the carotenoid emulsion sprayed into the heat exchange chamber for heat treatment freely falls into a feed liquid buffer chamber and then is sent into a homogenizing system for homogenizing treatment.
Preferably, the method further comprises the following steps: and (3) conveying the homogenized carotenoid emulsion to a vacuum chamber to start vacuumizing and dehydrating, and obtaining the nano-scale carotenoid emulsion after the mass concentration of the carotenoid emulsion is reduced to 90-110% of the initial mass concentration.
Preferably, in the above method, in the step (2), the mass concentration of carotenoid in the carotenoid emulsion injected into the heat exchange chamber is 0.1 to 25% by weight.
Preferably, in the above method, in the step (2), the carotenoid emulsion sprayed into the heat exchange chamber is a carotenoid macroemulsion, and the particle size of the carotenoid in the carotenoid macroemulsion is not more than 12 μm;
preferably, the carotenoid crude emulsion is obtained by mixing carotenoid with oil-soluble antioxidant and optionally added oil and fat, adding the mixture into emulsion containing emulsifier and antioxidant under stirring, and emulsifying by high-speed grinding;
and/or, the carotenoid crude emulsion is obtained by mixing carotenoid with oil-soluble antioxidant and optionally added grease, adding the mixture into emulsion containing emulsifier, filler and antioxidant under stirring, and emulsifying by high-speed grinding;
more preferably, the emulsifier is selected from one or more of sodium caseinate, acacia, modified starch, soy protein isolate and whey protein;
and/or the antioxidant is selected from one or more of ascorbyl palmitate, sodium ascorbate, ascorbic acid, sodium erythorbate, phospholipid and vitamin E;
and/or the oil-soluble antioxidant is selected from one or more of ascorbyl palmitate, vitamin E and phospholipid;
and/or the filling agent is selected from one or more of glucose, lactose and maltodextrin;
and/or the grease is selected from vegetable oils, such as soybean oil, peanut oil, sunflower seed oil, and the like.
Preferably, in the above method, the carotenoid is one or more selected from the group consisting of beta-carotene, lycopene, astaxanthin, lutein, zeaxanthin and canthaxanthin.
Preferably, in the above method, the carotenoid emulsion is a coarse emulsion with the particle size of less than 12 microns prepared by adding a mixture obtained by mixing carotenoid with the mass ratio of 1-25:0.01-3:1-40, antioxidant and emulsifier into water (the mass ratio of the mixture to water is 1:0.7-10), shearing and grinding, wherein the emulsifier is one or more of sodium caseinate, acacia, modified starch, soy isolated protein and whey protein, more preferably acacia and/or modified starch; the antioxidant is ascorbyl palmitate, sodium ascorbate, ascorbic acid, sodium erythorbate or vitamin E, more preferably sodium ascorbate or sodium erythorbate.
Preferably, in the above method, in the process of vacuumizing and dehydrating, the absolute pressure in the vacuum chamber is 10-55 kPa.
The invention also provides a nano carotenoid emulsion which is prepared by the device or the method. According to the invention, industrial steam is converted into clean steam through the clean steam generator and then is continuously introduced into the heat exchange chamber, non-condensable air in the heat exchange chamber is discharged through the emptying pipe, and the emptying pneumatic valve is closed after the non-condensable air is discharged. And (3) when the pressure of the heat exchange chamber is increased to 5-15bar, spraying the micron-sized carotenoid emulsion into the heat exchange chamber through a spray outlet of a spray nozzle to start heat treatment, and continuously introducing clean steam in the process to ensure that the pressure in the chamber is kept stable. The clean steam condenses on the surface of the fluid to release latent heat, the carotenoid emulsion is heated to 160-200 ℃ for 0.1-1s, the carotenoid crystals are in a molten fluid state in the temperature range, and the carotenoid emulsion in the molten state freely falls into the feed liquid buffer chamber.
And after the carotenoid emulsion stays in a feed liquid buffer chamber for 1-10 seconds to ensure that the carotenoid is completely melted, the carotenoid emulsion is sent into a homogenizing system (a high-pressure homogenizer) for homogenizing (the primary homogenizing pressure is 40-50 MPa, and the secondary homogenizing pressure is 6-8 MPa), the homogenized material enters a vacuum chamber according to the concentration requirement, a vacuum pump is started to maintain the absolute pressure in the vacuum chamber to be 10-55 kPa for dehydration, the carotenoid emulsion can be boiled under the vacuum condition and rapidly reduced to 50-80 ℃, the moisture absorbed by the early heat exchange is evaporated, and the nano-level carotenoid emulsion is obtained after the mass concentration of the carotenoid emulsion is reduced to be within 10% of the mass concentration relative difference of the crude solution.
The nano carotenoid emulsion obtained by the invention can be used as intermediate raw materials of foods, cosmetics and the like or can be directly applied to final products, and can also be added with components such as filling agents such as glucose, lactose, maltodextrin and the like, and the carotenoid microcapsule is obtained after spray drying.
The beneficial effects obtained by the invention are as follows:
the device and the method for producing the nano carotenoid emulsion provided by the invention can prepare the nano carotenoid emulsion with high content and uniform dispersion without using an organic solvent; the heat treatment time of the carotenoid emulsion is short, the influence on the structure of the carotenoid emulsion caused by long-time heating is avoided, the content of trans-carotenoid in the carotenoid nano emulsion with a high trans-structure is ensured, and the carotenoid nano emulsion is suitable for industrial mass production.
Drawings
FIG. 1 is a flow chart of the process for producing nano-scale carotenoid emulsions in example 1, the labels in the figure are as follows: 1-heat exchange chamber, 2-feed liquid buffer chamber, 3-homogenizing system, 4-vacuum chamber, 5-evacuation pipe, 6-clean steam generator, and 7-vacuum pump.
Figure 2 is an HPLC profile of a beta-carotene feedstock.
FIG. 3 is an HPLC chart of the beta-carotene emulsion prepared in example 1.
FIG. 4 is a graph showing the particle size of beta-carotene in the beta-carotene emulsion prepared in example 1.
FIG. 5 is an HPLC chart of the beta-carotene emulsion prepared in comparative example 1.
Detailed Description
The present invention will be further described in detail with reference to specific examples, in which an emulsion of beta-carotene is used as an example for better embodying the object of the present invention, and the properties of carotenoids are similar, and the replacement of carotenoid bodies such as lycopene, astaxanthin, lutein, zeaxanthin and the like does not affect the achievement of the object of the present invention, nor should the scope of the present invention be limited by beta-carotene.
The experimental methods used in the following examples are conventional methods unless otherwise specified. The experimental materials and related equipment used in the examples below, unless otherwise specified, are all commercially available.
The beta-carotene raw material used in the following examples was a beta-carotene product produced and sold by Jiabiyu biotechnology (Wuhan) Inc., wherein the content of trans beta-carotene was 97% or more, and FIG. 2 is an HPLC chart of the beta-carotene raw material. The detection conditions were as follows: a wavelength 453nm; the flow rate is 0.5mL/min; mobile phase: phase a methanol, phase B acetonitrile: methanol: isopropanol is configured in a ratio of 455:450:20; the elution procedure was: after eluting the phase B for 30min, adopting A: the elution was continued with a ratio of B of 16:84.
Example 1
The embodiment provides a device for producing nano-grade beta-carotene emulsion, which comprises a heat exchange chamber 1, a feed liquid buffer chamber 2, a homogenizing system 3 and a vacuum chamber 4 which are sequentially connected as shown in figure 1.
The heat exchange chamber 1 is characterized in that the height of the interior of the heat exchange chamber 1 is 1.33m, a spray nozzle is arranged at the top in the heat exchange chamber 1, 4 spray outlets are formed in the spray nozzle and are distributed in square four corners, the side length of the square is 50mm, the aperture of any spray outlet is 1.5mm, and the spray nozzle is used for conical spraying of beta-carotene emulsion into the heat exchange chamber 1. In the embodiment, the flow rate of the beta-carotene emulsion is designed to be 300L/h according to the working requirement.
An emptying pipe 5 is arranged on the heat exchange chamber 1, and a pneumatic valve and a temperature detection device for controlling the opening or closing of a pipeline of the emptying pipe 5 are arranged on the heat exchange chamber.
The device also comprises a clean steam generator 6 connected with the heat exchange chamber, wherein the clean steam generator 6 is used for converting industrial water steam into clean water steam and then sending the clean water steam into the heat exchange chamber 1.
The heat exchange chamber 1 is provided with a pressure sensor for monitoring the pressure in the chamber in real time.
The feed liquid buffer chamber 2 is arranged below the heat exchange chamber and is used for receiving beta-carotene emulsion flowing out of the heat exchange chamber 1.
The feed liquid buffer chamber 2 is provided with a capacitance type level gauge for monitoring the material liquid level.
The homogenizing system 3 is a high-temperature and high-pressure resistant homogenizer, and is of the type: SRH1000-90 for homogenizing beta-carotene emulsion.
The vacuum chamber 4 is connected to a vacuum pump 7 for partially removing water from the homogenized beta-carotene emulsion.
The embodiment also provides a method for producing beta-carotene emulsion by using the device, which comprises the following steps:
(1) Mixing the mixture obtained by mixing the beta-carotene, the sodium ascorbate and the modified starch in the mass ratio of 10:1:39 with water in the mass ratio of 1:9, and grinding to obtain the coarse emulsion with the beta-carotene mass content of 2wt% and the particle size of 2-8 microns.
(2) Industrial steam is converted into clean steam through the clean steam generator 6 and then is continuously introduced into the heat exchange chamber 1, non-condensable air in the heat exchange chamber is discharged through the exhaust pipe 5, and the exhaust pneumatic valve is closed after the non-condensable air is discharged. And (3) when the pressure of the heat exchange chamber 1 is increased to 5bar, the temperature in the heat exchange chamber 1 is 160 ℃, the micron-sized beta-carotene emulsion prepared in the step (1) is sprayed into the heat exchange chamber 1 through a spray nozzle cone to start heat treatment, and in the process, clean steam is continuously introduced to ensure that the pressure in the chamber is maintained at 5bar. The clean steam condenses on the surface of the fluid to release latent heat, the beta-carotene emulsion 1s is heated to 160 ℃, at the temperature, the beta-carotene crystal is in a molten fluid state, and the beta-carotene emulsion in the molten state falls into a feed liquid buffer chamber 2;
(3) Homogenizing the beta-carotene emulsion in a material liquid buffer chamber 2 for 5 seconds after the beta-carotene emulsion is completely melted by a homogenizing system 3, wherein the primary pressure of the homogenizing is 40MPa, the secondary pressure is 6MPa, the homogenized material enters a vacuum chamber 4, a vacuum pump 7 is started to maintain the vacuum degree of the vacuum chamber 4 at 70kPa for vacuum dehydration, the beta-carotene emulsion is boiled and rapidly cooled to 70 ℃ under the vacuum condition, and the beta-carotene emulsion is obtained after the beta-carotene mass content in the beta-carotene emulsion is reduced to 2wt%, wherein the trans-beta-carotene content is 1.94wt%.
FIG. 3 is an HPLC chart of the nanoscale beta-carotene emulsion prepared in example 1. As can be seen from fig. 3, the trans structure of β -carotene in the nano-scale β -carotene emulsion prepared in example 1 was not destroyed.
Fig. 4 is a graph showing the particle size of the nano-sized β -carotene emulsion prepared in example 1, and it is clear from fig. 4 that the particle size of β -carotene in the nano-sized β -carotene emulsion prepared in example 1 reaches the nano-sized level.
From this, it can be seen that the apparatus and method for producing nano-scale beta-carotene emulsion provided in example 1 not only achieves the effect of melt, homogenization and dispersion of beta-carotene, but also retains the structure of the trans-active ingredient in beta-carotene.
Example 2
The apparatus for producing nano-sized beta-carotene emulsion provided in this example is the same as in example 1,
the present example provides a method for producing a beta-carotene emulsion using the apparatus described above, comprising the steps of:
(1) Mixing the mixture obtained by mixing the beta-carotene, sodium ascorbate and acacia in the mass ratio of 20:1:19 with water in the mass ratio of 1:1, and grinding to obtain the coarse emulsion with the beta-carotene mass content of 25wt% and the particle size of 4-10 microns.
(2) Industrial steam is converted into clean steam through the clean steam generator 6 and then is continuously introduced into the heat exchange chamber 1, non-condensable air in the heat exchange chamber is discharged through the exhaust pipe 5, and the exhaust pneumatic valve is closed after the non-condensable air is discharged. And (3) when the pressure of the heat exchange chamber 1 is increased to 10bar, the temperature in the heat exchange chamber is 180 ℃, the micron-sized beta-carotene emulsion prepared in the step (1) is sprayed into the heat exchange chamber 1 through a spray nozzle cone to start heat treatment, and in the process, clean steam is continuously introduced to ensure that the pressure in the chamber is maintained at 10bar. The clean steam condenses on the surface of the fluid to release latent heat, the beta-carotene emulsion 1s is heated to 180 ℃, the beta-carotene crystal is in a molten fluid state at the temperature, and the beta-carotene emulsion in the molten state falls into the feed liquid buffer chamber 2;
(3) The beta-carotene emulsion is subjected to homogenization through a homogenizing system 3 after being completely melted for 10 seconds in a feed liquid buffer chamber 2, the primary pressure of homogenization is 50MPa, the secondary pressure is 8MPa, the homogenized material enters a vacuum chamber 4, a vacuum pump 7 is started to maintain the vacuum degree of the vacuum chamber 4 at 88kPa for vacuum dehydration, the beta-carotene emulsion is boiled under the vacuum condition and rapidly reduced to 50 ℃, the moisture absorbed by the prior heat exchange is evaporated, and the nanoscale beta-carotene emulsion is obtained after the beta-carotene mass content in the beta-carotene emulsion is 25wt%, wherein the trans beta-carotene content is 24.25wt%.
Comparative example 1
Comparative example 1 beta-carotene emulsion was prepared by a conventional melting method, in which beta-carotene was added to vegetable oil and dispersed by shearing and homogenizing after melting, the specific steps were as follows:
(1) Heating beta-carotene and sunflower seed oil with the mass ratio of 1:4 to 160 ℃ for standby under the stirring state;
(2) Mixing sodium ascorbate and modified starch in a mass ratio of 1:39, and adding the obtained mixture into water in a mass ratio of 4:86 for stirring and dissolving;
(3) The solutions of step (1) and step (2) are mixed according to the following formula 1:9 in a shear state. Homogenizing the sheared and dispersed emulsion by a high-pressure homogenizer (the primary homogenizing pressure is 40-50 MPa, and the secondary homogenizing pressure is 6-8 MPa) to obtain the beta-carotene emulsion with the mass content of 2%.
FIG. 5 is an HPLC chart of the beta-carotene emulsion prepared in comparative example 1. As can be seen from fig. 5, the trans structure of beta-carotene in the beta-carotene emulsion prepared in comparative example 1 was destroyed.
From this, it is clear that the conventional melting method provided in comparative example 1 produces beta-carotene emulsion, and the trans structure of the beta-carotene product is destroyed due to long heating time, and the content of beta-carotene is not easily increased (due to the addition of vegetable oil) in the conventional melting method used in comparative example 1, but only 1 to 2%.
While the invention has been described in detail in the foregoing general description, embodiments and experiments, it will be apparent to those skilled in the art that modifications and improvements can be made thereto. Accordingly, such modifications or improvements may be made without departing from the spirit of the invention and are intended to be within the scope of the invention as claimed.

Claims (5)

1. A method for producing nano-scale carotenoid emulsions using an apparatus, characterized in that the apparatus comprises a heat exchange chamber in which carotenoids in carotenoid emulsions injected therein are heat-treated by water vapor;
the homogenizing system is used for homogenizing the carotenoid emulsion after the heat treatment;
a feed liquid buffer chamber arranged between the heat exchange chamber and the homogenizing system and used for receiving carotenoid emulsion flowing out of the heat exchange chamber;
a vacuum chamber for partially removing water from the homogenized carotenoid emulsion;
a spray nozzle is arranged in the heat exchange chamber, the spray nozzle comprises a plurality of spray outlets, carotenoid emulsion is sprayed into the heat exchange chamber through the spray outlets in a conical manner, the aperture of any spray outlet is 0.8-2 mm, and the interval between any two adjacent spray outlets is 40-60 mm;
the method comprises the following steps:
(1) Adding water vapor into the heat exchange chamber and maintaining the pressure in the heat exchange chamber to be 5-15bar;
(2) Spraying carotenoid emulsion with a certain initial mass concentration into the heat exchange chamber to perform heat treatment on carotenoid, and sending the carotenoid emulsion after heat treatment into a homogenizing system to perform homogenizing treatment; the carotenoid emulsion is a coarse emulsion prepared by adding a mixture obtained by mixing carotenoid with the mass ratio of 1-25:0.01-3:1-40, an antioxidant and an emulsifier into water, shearing and grinding the mixture; the mass ratio of the mixture to water is 1:0.7-10;
the method also comprises the following steps: the carotenoid emulsion sprayed into the heat exchange chamber for heat treatment freely falls into a feed liquid buffer chamber and then is sent into a homogenizing system for homogenizing treatment;
and (3) conveying the homogenized carotenoid emulsion to a vacuum chamber to start vacuumizing and dehydrating, and obtaining the nano-scale carotenoid emulsion after the mass concentration of the carotenoid emulsion is reduced to 90-110% of the initial mass concentration.
2. The method of claim 1, wherein the heat exchange chamber is provided with an evacuation pipe, and the evacuation pipe is provided with a pneumatic valve for controlling opening or closing of a pipeline thereof.
3. The method of claim 1 or 2, wherein the apparatus further comprises a clean steam generator connected to the heat exchange chamber, the clean steam generator being adapted to convert industrial water vapour to clean water vapour before feeding into the heat exchange chamber.
4. The method of claim 1, wherein the absolute pressure in the vacuum chamber is 10-55 kpa during the vacuum dehydration.
5. A nano-scale carotenoid emulsion prepared by the method of any one of claims 1-4.
CN202010550602.XA 2020-06-16 2020-06-16 Device and method for producing nano carotenoid emulsion Active CN111838648B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010550602.XA CN111838648B (en) 2020-06-16 2020-06-16 Device and method for producing nano carotenoid emulsion

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010550602.XA CN111838648B (en) 2020-06-16 2020-06-16 Device and method for producing nano carotenoid emulsion

Publications (2)

Publication Number Publication Date
CN111838648A CN111838648A (en) 2020-10-30
CN111838648B true CN111838648B (en) 2023-04-25

Family

ID=72986679

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010550602.XA Active CN111838648B (en) 2020-06-16 2020-06-16 Device and method for producing nano carotenoid emulsion

Country Status (1)

Country Link
CN (1) CN111838648B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115153018B (en) * 2022-07-04 2023-07-21 新昌新和成维生素有限公司 Production method and production system of carotenoid emulsion

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0410236B1 (en) * 1989-07-25 1993-10-27 F. Hoffmann-La Roche Ag Process for the production of carotinoid preparations
EP0807431B1 (en) * 1996-05-14 2004-03-24 DSM IP Assets B.V. Process for the manufacture of carotenoid compositions
CN108030063A (en) * 2017-12-17 2018-05-15 江南大学 A kind of preparation method of the beta carotene microcapsules of high carrying capacity high bioavilability
CN108148444B (en) * 2017-12-29 2020-08-11 嘉必优生物技术（武汉）股份有限公司 Preparation method of high-content beta-carotene microcapsule for coloring

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
朱明军等.生物工程设备.《生物工程设备》.中国轻工业出版社,2019,第205页. *
食品营养强化剂.曹劲松、王晓琴.《食品营养强化剂》.中国轻工业出版社,2002,第304-305页. *

Also Published As

Publication number Publication date
CN111838648A (en) 2020-10-30

Similar Documents

Publication Publication Date Title
JP6099354B2 (en) Direct dissolution
US5863953A (en) Liquid, oil-miscible carotenoid preparations
EP1794238B1 (en) Method for producing dry powders of at least one carotenoid
EP0937412B1 (en) Preparation of a finely divided pulverous carotenoid preparation
EP2224823B1 (en) Pulverulent carotenoid preparation for colouring drinks
JPH0366615A (en) Manufacture of new colloid dispersion carotinoid preparation
WO2010112406A1 (en) Ready-to-use, stable suspension of partially amorphous carotenoid particles
WO2007003543A1 (en) Method for producing an aqueous suspension and a powdered preparation of one or more carotinoids
CN111838648B (en) Device and method for producing nano carotenoid emulsion
DK2222180T3 (en) METHOD OF PREPARING CAROTENOID SOLUTIONS
CN112056558A (en) Oil-in-water carotene microemulsion and preparation method thereof
EP2403361B1 (en) Method for producing carotenoid solutions
CN113598304A (en) Method for preparing carotenoid
CN101879428B (en) Method for preparing carotenoid microcapsules with controllable isomer proportion
US20040267033A1 (en) Extraction of lutein from marigold meal
JP4630545B2 (en) Dispersion manufacturing process
JP2002112712A (en) Stable carotenoid-containing powder and method for producing the same
WO2009065928A1 (en) Method for producing carotenoid solutions
CN113841895A (en) Preparation method of beta-carotene microcapsule powder
EP4421064A1 (en) A preparation method of carotenoid agent
DE102008058446A1 (en) Producing carotenoid-solutions in an edible oil, comprises providing carotenoid suspension in oil, heating oil stream comprising suspension and further edible oil, to produce a hot carotenoid-solution and cooling hot carotenoid-solution
DE102008058447A1 (en) Producing carotenoid-solutions in edible oil, comprises providing a carotenoid suspension in the oil, heating a stream of suspension in edible oil to obtain a hot carotenoid-solution and cooling the hot carotenoid-solution
DE102008058449A1 (en) Producing carotenoid-solutions in edible oil, comprises providing a carotenoid suspension in oil, heating a stream of the suspension in edible oil to produce a hot carotenoid-solution and cooling the hot carotenoid-solution

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant