CN114286861B - 捕获和分析靶基因组区域 - Google Patents

捕获和分析靶基因组区域 Download PDF

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Publication number
CN114286861B
CN114286861B CN202080051127.XA CN202080051127A CN114286861B CN 114286861 B CN114286861 B CN 114286861B CN 202080051127 A CN202080051127 A CN 202080051127A CN 114286861 B CN114286861 B CN 114286861B
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Prior art keywords
target genomic
genomic region
target
recognition site
region
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Chinese (zh)
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CN114286861A (zh
Inventor
莱昂德罗·戈米德·内维斯
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Lgc Genomics Co ltd
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LGC Genomics LLC
Rapid Genomics LLC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202080051127.XA 2019-05-13 2020-05-13 捕获和分析靶基因组区域 Active CN114286861B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962846988P 2019-05-13 2019-05-13
US62/846,988 2019-05-13
PCT/US2020/032615 WO2020232081A2 (en) 2019-05-13 2020-05-13 Capture and analysis of target genomic regions

Publications (2)

Publication Number Publication Date
CN114286861A CN114286861A (zh) 2022-04-05
CN114286861B true CN114286861B (zh) 2024-12-06

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CN202080051127.XA Active CN114286861B (zh) 2019-05-13 2020-05-13 捕获和分析靶基因组区域

Country Status (10)

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US (1) US20220220542A1 (https=)
EP (1) EP3969581B1 (https=)
JP (1) JP7854386B2 (https=)
KR (1) KR20220039653A (https=)
CN (1) CN114286861B (https=)
AU (1) AU2020275301A1 (https=)
BR (1) BR112022001539A2 (https=)
CA (1) CA3145806A1 (https=)
ES (1) ES3051342T3 (https=)
WO (1) WO2020232081A2 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4034675A4 (en) * 2019-09-26 2023-10-04 Jumpcode Genomics, Inc. Method and system for targeted nucleic acid sequencing
WO2022232425A2 (en) * 2021-04-29 2022-11-03 Illumina, Inc. Amplification techniques for nucleic acid characterization

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106661631A (zh) * 2014-06-06 2017-05-10 康奈尔大学 使用组合的核酸酶、连接酶、聚合酶和测序反应识别和枚举核酸序列、表达、拷贝或dna甲基化变化的方法
CN114207229A (zh) * 2019-05-30 2022-03-18 快速基因组学有限责任公司 靶基因组区域的灵活且高通量的测序

Family Cites Families (13)

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FR2876249A1 (fr) 2004-10-11 2006-04-14 Barry Callebaut Ag Utilisation du beurre de cacao dans des preparations culinaires
US20090233291A1 (en) * 2005-06-06 2009-09-17 454 Life Sciences Corporation Paired end sequencing
CA2615323A1 (en) * 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
EP3257949A1 (en) 2005-06-15 2017-12-20 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
CN101432439B (zh) * 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
US20090156412A1 (en) 2007-12-17 2009-06-18 Helicos Biosciences Corporation Surface-capture of target nucleic acids
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9365897B2 (en) * 2011-02-08 2016-06-14 Illumina, Inc. Selective enrichment of nucleic acids
US9873907B2 (en) * 2013-05-29 2018-01-23 Agilent Technologies, Inc. Method for fragmenting genomic DNA using CAS9
HRP20200529T1 (hr) * 2014-06-06 2020-09-04 Regeneron Pharmaceuticals, Inc. Postupci i pripravci za modificiranje ciljnog lokusa
WO2016109255A1 (en) * 2014-12-30 2016-07-07 University Of South Florida Methods and compositions for cloning into large vectors
GB2565461B (en) * 2016-03-31 2022-04-13 Harvard College Methods and compositions for the single tube preparation of sequencing libraries using Cas9
GB201616590D0 (en) * 2016-09-29 2016-11-16 Oxford Nanopore Technologies Limited Method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106661631A (zh) * 2014-06-06 2017-05-10 康奈尔大学 使用组合的核酸酶、连接酶、聚合酶和测序反应识别和枚举核酸序列、表达、拷贝或dna甲基化变化的方法
CN114207229A (zh) * 2019-05-30 2022-03-18 快速基因组学有限责任公司 靶基因组区域的灵活且高通量的测序

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Recent advances in rolling circle amplification-based biosensing strategies-A review;Lulu Xu等;《Analytica Chimica Acta》;20210301;全文 *

Also Published As

Publication number Publication date
BR112022001539A2 (pt) 2022-03-22
ES3051342T3 (en) 2025-12-26
EP3969581B1 (en) 2025-10-08
JP2022534625A (ja) 2022-08-02
WO2020232081A3 (en) 2020-12-24
AU2020275301A1 (en) 2022-01-20
EP3969581A2 (en) 2022-03-23
EP3969581A4 (en) 2023-06-14
WO2020232081A2 (en) 2020-11-19
US20220220542A1 (en) 2022-07-14
KR20220039653A (ko) 2022-03-29
CN114286861A (zh) 2022-04-05
CA3145806A1 (en) 2020-11-19
JP7854386B2 (ja) 2026-05-01

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