CN114276956A - Microbial agent and application thereof - Google Patents
Microbial agent and application thereof Download PDFInfo
- Publication number
- CN114276956A CN114276956A CN202111547645.3A CN202111547645A CN114276956A CN 114276956 A CN114276956 A CN 114276956A CN 202111547645 A CN202111547645 A CN 202111547645A CN 114276956 A CN114276956 A CN 114276956A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- parts
- microbial agent
- microbial
- beauveria bassiana
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000000813 microbial effect Effects 0.000 title claims abstract description 75
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 60
- 239000000843 powder Substances 0.000 claims abstract description 29
- 238000002360 preparation method Methods 0.000 claims abstract description 22
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 21
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 21
- 241000193388 Bacillus thuringiensis Species 0.000 claims abstract description 21
- 241000751139 Beauveria bassiana Species 0.000 claims abstract description 21
- 229940097012 bacillus thuringiensis Drugs 0.000 claims abstract description 21
- 239000000203 mixture Substances 0.000 claims abstract description 16
- 238000000855 fermentation Methods 0.000 claims description 95
- 230000004151 fermentation Effects 0.000 claims description 95
- 239000007788 liquid Substances 0.000 claims description 26
- 230000001580 bacterial effect Effects 0.000 claims description 16
- 239000001963 growth medium Substances 0.000 claims description 15
- 238000011081 inoculation Methods 0.000 claims description 14
- 239000003463 adsorbent Substances 0.000 claims description 13
- 238000002156 mixing Methods 0.000 claims description 11
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- 239000002054 inoculum Substances 0.000 claims description 8
- FYGDTMLNYKFZSV-DZOUCCHMSA-N alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-D-Glcp Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)O[C@H](O[C@@H]2[C@H](OC(O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-DZOUCCHMSA-N 0.000 claims description 7
- 238000001694 spray drying Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 5
- 229920002261 Corn starch Polymers 0.000 claims description 5
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 5
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- 239000001110 calcium chloride Substances 0.000 claims description 5
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 5
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 5
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 5
- 239000008120 corn starch Substances 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- 229940099596 manganese sulfate Drugs 0.000 claims description 5
- 239000011702 manganese sulphate Substances 0.000 claims description 5
- 235000007079 manganese sulphate Nutrition 0.000 claims description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 5
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000003610 charcoal Substances 0.000 claims description 4
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 2
- 244000005700 microbiome Species 0.000 abstract description 21
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 abstract description 18
- 239000002689 soil Substances 0.000 abstract description 18
- 230000000694 effects Effects 0.000 abstract description 8
- 230000002265 prevention Effects 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 4
- 244000088415 Raphanus sativus Species 0.000 abstract 1
- 241000220259 Raphanus Species 0.000 description 17
- 230000000052 comparative effect Effects 0.000 description 15
- 238000000034 method Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 244000052616 bacterial pathogen Species 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000003337 fertilizer Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000006467 substitution reaction Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241000223221 Fusarium oxysporum Species 0.000 description 2
- 230000002860 competitive effect Effects 0.000 description 2
- 239000002068 microbial inoculum Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a microbial agent and application thereof, wherein the microbial agent comprises bacillus thuringiensis, beauveria bassiana and bacillus subtilis. The application of the microbial agent is specifically application in preventing and treating crop black mold. The microbial agent contains three microorganisms, namely bacillus thuringiensis, beauveria bassiana and bacillus subtilis, and has the advantage of simple composition; when the microbial agent is applied to prevention and treatment of the black mold of radish, the morbidity of the black mold can be obviously reduced, and in addition, the preparation method of the microbial powder provided by the invention can enable three microorganisms to be better distributed in soil, so that the propagation rate of the microorganisms is further improved, and the prevention and treatment effect of the microbial agent on the black mold is more obvious.
Description
Technical Field
The invention relates to the technical field of microbial fertilizers, and particularly relates to a microbial agent and application thereof.
Background
In recent years, with the improvement of the knowledge of the microbial fertilizer, the research on the microbial agent is more and more. The microbial agent can be used as a microbial agent alone or mixed with a fertilizer to be used as a fertilizer, and the microbes acting on soil and the microbes acting on the soil are all the microbes in the microbial agent in any use mode, so the composition of the microbial agent is a key factor for exerting the biological activity of the microbial agent.
The microorganism plays an important role in crop planting, and has the following main functions: providing nutrients for the growth of crops by decomposing organic matters in soil; after the organic matters are decomposed, the structure of the soil is changed, the permeability of the soil is improved, the growth of the root system of the crops is promoted, and the yield of the crops is improved; in addition, the method can also inhibit harmful bacteria in soil through the competitive action among microorganisms, so that the harmful bacteria are prevented from harming crops, and the growth and the yield of the crops are further ensured.
The black rot is a crop disease caused by fusarium oxysporum, the fusarium oxysporum causing the black rot can be enriched and acted in soil, the caused black rot is gradually infected from the lower leaves of the crops to the upper leaves, the infection speed is high, and the infected crops are almost in a dead-end state. At present, in order to solve the influence of black rot on crops, various microbial agents appear in the market, however, the treatment effect on the black rot is not ideal, which may be because various microorganisms are compounded in the microbial agents, but because soil has a certain microbial environment, after too many microorganisms enter the soil, the microbial equilibrium state in the soil is broken, and the control effect on pathogenic bacteria is poor due to the competitive action among the microorganisms, so that the provision of the microbial agent which is simple in composition and can adapt to the soil environment quickly has important significance for the research on the microbial agents.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a microbial agent and application thereof, wherein the microbial agent comprises three microorganisms, namely bacillus thuringiensis, beauveria bassiana and bacillus subtilis, and has the advantage of simple composition; when the microbial agent is applied to prevention and treatment of the black mold of radish, the morbidity of the black mold can be obviously reduced, and in addition, the preparation method of the microbial powder provided by the invention can enable three microorganisms to be better distributed in soil, so that the propagation rate of the microorganisms is further improved, and the prevention and treatment effect of the microbial agent on the black mold is more obvious.
The technical scheme of the invention is as follows:
a microbial agent comprises Bacillus thuringiensis, Beauveria bassiana and Bacillus subtilis.
Furthermore, the viable count in the microbial inoculum is more than or equal to 10.0 hundred million/g.
Further, each 100 parts of the microbial agent comprises the following components in parts by weight:
20-30 parts of bacillus thuringiensis, 10-15 parts of beauveria bassiana, 25-30 parts of bacillus subtilis, 20-35 parts of oligomeric maltose and 5-10 parts of an adsorbent.
Further, each 100 parts of the microbial agent comprises the following components in parts by weight:
25 parts of bacillus thuringiensis, 13 parts of beauveria bassiana, 27 parts of bacillus subtilis, 25 parts of malto-oligosaccharide and 10 parts of adsorbent.
Further, the adsorbent is charcoal.
Further, the microbial agent is specifically a microbial agent obtained by mixing bacterial powder and maltooligosaccharide.
Further, in the microbial agent, the preparation method of the bacterial powder comprises the following steps:
(1) the preparation method of the bacillus thuringiensis fermentation liquor comprises the following steps: inoculating Bacillus thuringiensis seed liquid into a fermentation culture medium according to the inoculation amount of 3%, controlling the fermentation temperature to be 28 +/-0.2 ℃, and when the viable count in the fermentation liquid reaches 1 multiplied by 1010-2×1010Stopping fermentation when cfu/g;
(2) the preparation method of the beauveria bassiana fermentation liquor comprises the following steps: inoculating the beauveria bassiana seed liquid into a fermentation culture medium according to the inoculation amount of 5 percentControlling the fermentation temperature to 26 +/-0.2 ℃, and when the viable count in the fermentation liquor reaches 1 multiplied by 1010-2×1010Stopping fermentation when cfu/g;
(3) the preparation method of the bacillus subtilis fermentation liquor comprises the following steps: inoculating the Bacillus subtilis seed solution into a fermentation culture medium according to the inoculation amount of 7%, controlling the fermentation temperature to be 35 +/-0.2 ℃, and when the viable count in the fermentation liquid reaches 1 multiplied by 1010-2×1010Stopping fermentation when cfu/g;
(4) mixing the fermentation liquors obtained in the steps (1), (2) and (3), adding an adsorbent, stirring, standing for 24-36h, and spray drying to obtain bacterial powder; the setting of the standing process can make the microorganisms attached to the adsorbent.
Further, the composition of the fermentation medium was as follows:
2030g of corn starch, 1520g of bean cake powder, 56g of yeast powder, 0.50.7g of magnesium sulfate, 0.2-0.4g of calcium chloride, 0.05-0.1g of manganese sulfate, 0.4-0.5g of monopotassium phosphate, 0.07-0.1g of copper sulfate and 1000ml of distilled water, and sterilizing at 121 ℃ for 30 min.
The microbial agent is applied, in particular to the application of the microbial agent in preventing and treating the crop black mold.
Compared with the prior art, the invention has the beneficial effects that:
1. the microbial agent provided by the invention comprises three microorganisms, namely bacillus thuringiensis, beauveria bassiana and bacillus subtilis, and has the advantage of simple composition. Experiments show that the three microorganisms can quickly adapt to the soil environment in the soil and then propagate, and the method has the advantages of high practicability and good prevention effect.
2. The microbial agent provided by the invention can be used for remarkably reducing the incidence rate of the black mold when being applied to the black mold of radish, and in addition, the preparation method of the microbial powder provided by the invention can enable three microorganisms to be better distributed in soil, so that the propagation rate of the microorganisms is further improved, and further, the prevention and treatment effect of the microbial agent on the black mold is more remarkable.
Detailed Description
In order to make those skilled in the art better understand the technical solutions in the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
When 100kg of microbial agent is prepared, the preparation method of the bacterial powder comprises the following steps:
(1) the preparation method of the bacillus thuringiensis fermentation liquor comprises the following steps: inoculating Bacillus thuringiensis seed liquid into a fermentation culture medium according to the inoculation amount of 3%, controlling the fermentation temperature to be 28 +/-0.2 ℃, and when the viable count in the fermentation liquid reaches 1.5 multiplied by 1010Stopping fermentation when cfu/g;
(2) the preparation method of the beauveria bassiana fermentation liquor comprises the following steps: inoculating the Beauveria bassiana seed liquid into a fermentation culture medium according to the inoculation amount of 5%, controlling the fermentation temperature to be 26 +/-0.2 ℃, and when the viable count in the fermentation liquid reaches 1.5 multiplied by 1010Stopping fermentation when cfu/g;
(3) the preparation method of the bacillus subtilis fermentation liquor comprises the following steps: inoculating the Bacillus subtilis seed solution into a fermentation culture medium according to the inoculation amount of 7%, controlling the fermentation temperature to be 35 +/-0.2 ℃, and when the viable count in the fermentation liquid reaches 1.5 multiplied by 1010When the fermentation is finished, stopping fermentation;
the composition of the fermentation medium was as follows:
25g of corn starch, 17g of bean cake powder, 5.5g of yeast powder, 0.6g of magnesium sulfate, 0.3g of calcium chloride, 0.07g of manganese sulfate, 0.45g of monopotassium phosphate, 0.09g of copper sulfate and 1000ml of distilled water, and sterilizing at 121 ℃ for 30 min.
(4) Mixing 25kg of fermentation liquor obtained in the step (1), 13kg of fermentation liquor obtained in the step (2) and 27kg of fermentation liquor obtained in the step (3), adding 10kg of charcoal into the mixture, stirring, standing for 32 hours, and performing spray drying to obtain bacterial powder; the setting of the standing process can lead the microorganisms to be attached to the adsorbent;
adding 25kg of maltooligosaccharide into the bacterial powder, and uniformly mixing to obtain the microbial agent.
Example 2
When 100kg of microbial agent is prepared, the preparation method of the bacterial powder comprises the following steps:
(1) the preparation method of the bacillus thuringiensis fermentation liquor comprises the following steps: inoculating Bacillus thuringiensis seed liquid into a fermentation culture medium according to the inoculation amount of 3%, controlling the fermentation temperature to be 28 +/-0.2 ℃, and when the viable count in the fermentation liquid reaches 1.0 multiplied by 1010Stopping fermentation when cfu/g;
(2) the preparation method of the beauveria bassiana fermentation liquor comprises the following steps: inoculating the Beauveria bassiana seed liquid into a fermentation culture medium according to the inoculation amount of 5%, controlling the fermentation temperature to be 26 +/-0.2 ℃, and when the viable count in the fermentation liquid reaches 1.0 multiplied by 1010Stopping fermentation when cfu/g;
(3) the preparation method of the bacillus subtilis fermentation liquor comprises the following steps: inoculating the Bacillus subtilis seed solution into a fermentation culture medium according to the inoculation amount of 7%, controlling the fermentation temperature to be 35 +/-0.2 ℃, and when the viable count in the fermentation liquid reaches 1.0 multiplied by 1010When the fermentation is finished, stopping fermentation;
the composition of the fermentation medium was as follows:
20g of corn starch, 15g of bean cake powder, 5g of yeast powder, 0.5g of magnesium sulfate, 0.2g of calcium chloride, 0.05g of manganese sulfate, 0.4g of monopotassium phosphate, 0.07g of copper sulfate and 1000ml of distilled water, and sterilizing at 121 ℃ for 30 min.
(4) Mixing 20kg of fermentation liquor obtained in the step (1), 10kg of fermentation liquor obtained in the step (2) and 25kg of fermentation liquor obtained in the step (3), adding 10kg of charcoal into the mixture, stirring, standing for 24 hours, and performing spray drying to obtain bacterial powder; the setting of the standing process can lead the microorganisms to be attached to the adsorbent;
adding 35kg of maltooligosaccharide into the bacterial powder, and uniformly mixing to obtain the microbial agent.
Example 3
When 100kg of microbial agent is prepared, the preparation method of the bacterial powder comprises the following steps:
(1) the preparation method of the bacillus thuringiensis fermentation liquor comprises the following steps: inoculating Bacillus thuringiensis with 3% of inoculum sizeInoculating the seed liquid into fermentation culture medium, controlling fermentation temperature at 28 + -0.2 deg.C, and allowing viable bacteria in the fermentation liquid to reach 2.0 × 1010Stopping fermentation when cfu/g;
(2) the preparation method of the beauveria bassiana fermentation liquor comprises the following steps: inoculating the Beauveria bassiana seed liquid into a fermentation culture medium according to the inoculation amount of 5%, controlling the fermentation temperature to be 26 +/-0.2 ℃, and when the viable count in the fermentation liquid reaches 2.0 multiplied by 1010Stopping fermentation when cfu/g;
(3) the preparation method of the bacillus subtilis fermentation liquor comprises the following steps: inoculating the Bacillus subtilis seed solution into a fermentation culture medium according to the inoculation amount of 7%, controlling the fermentation temperature to be 35 +/-0.2 ℃, and when the viable count in the fermentation liquid reaches 2.0 multiplied by 1010When the fermentation is finished, stopping fermentation;
the composition of the fermentation medium was as follows:
30g of corn starch, 20g of bean cake powder, 6g of yeast powder, 0.7g of magnesium sulfate, 0.4g of calcium chloride, 0.1g of manganese sulfate, 0.5g of monopotassium phosphate, 0.1g of copper sulfate and 1000ml of distilled water, and sterilizing at 121 ℃ for 30 min.
(4) Mixing 30kg of fermentation liquor obtained in the step (1), 15kg of fermentation liquor obtained in the step (2) and 30kg of fermentation liquor obtained in the step (3), adding 5kg of biochar into the mixture, stirring, standing for 24 hours, and performing spray drying to obtain bacterial powder; the setting of the standing process can lead the microorganisms to be attached to the adsorbent;
adding 20kg of malto oligosaccharide into the bacterial powder, and uniformly mixing to obtain the microbial agent.
Comparative example 1
The difference from example 1 is that no Bacillus thuringiensis was added.
Comparative example 2
The difference from example 1 is that beauveria bassiana was not added.
Comparative example 3
The difference from example 1 is that no Bacillus subtilis was added.
Comparative example 4
The difference from example 1 is that the process of step (4) is: and (3) mixing the fermentation liquors obtained in the steps (1), (2) and (3), adding an adsorbent into the mixture, uniformly stirring the mixture, and performing spray drying to obtain the bacterial powder.
Experiment 1 application of microbial agent in radish black mold
Performing a radish black mold experiment in a potting mode, and putting black mold pathogenic bacteria into soil in advance; each experimental area is 0.5 mu, and microbial inoculum is applied according to the amount of 100 kg/mu;
experimental group 1: applying the microbial agent of the example 1, and transplanting radish seedlings;
experimental group 2: applying the microbial agent of the embodiment 2, and transplanting radish seedlings;
experimental group 3: applying the microbial agent of the embodiment 3, and transplanting radish seedlings;
comparative group 1: applying the microbial agent of the comparative example 1, and transplanting radish seedlings;
comparative group 2: applying the microbial agent of the comparative example 2, and transplanting radish seedlings;
comparative group 3: applying the microbial agent of the comparative example 3, and transplanting the radish seedlings;
comparative group 4: applying the microbial agent of the comparative example 4, and transplanting the radish seedlings;
control group: no microbial agents were used.
The disease status of radish seedlings was counted after 30 days of transplantation for the pot culture of the experimental groups 1 to 3, the comparative groups 1 to 4 and the control group, and the results are shown in table 1.
TABLE 1 radish disease status
Incidence (%) — number of diseased plants/total number of plants × 100.
The combination of the table 1 shows that the incidence of the radish seedlings is obviously reduced after the microbial agent provided by the invention is used, which shows that the microbial agent provided by the invention can well inhibit pathogenic bacteria of the black mold, and the combination of the comparative examples 1-3 shows that the three microorganisms can generate a synergistic effect and jointly generate a powerful control effect on the pathogenic bacteria of the black mold.
Experiment 2
Taking radish seedlings in experimental communities of the experimental group 1 and the comparative group 4 at 0 day, 5 days, 10 days, 30 days and 60 days, washing the roots of the radish seedlings for 3 times, collecting washing liquid, and observing the number of viable bacteria in the washing liquid; 5 radish seedlings are taken from each cell, the average value of the viable count is taken, and the result is shown in table 2.
TABLE 2 statistics of viable counts
The combination of the table 2 shows that the microbial agent provided by the invention can rapidly reproduce in soil, which indicates that the microorganisms in the microbial agent have strong environmental adaptability, and the microbial agent can effectively generate control effect on black mold due to the strong adaptability.
Although the present invention has been described in detail by referring to the preferred embodiments, the present invention is not limited thereto. Various equivalent modifications or substitutions can be made on the embodiments of the present invention by those skilled in the art without departing from the spirit and scope of the present invention, and these modifications or substitutions are within the scope of the present invention/any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (9)
1. A microbial agent is characterized by comprising bacillus thuringiensis, beauveria bassiana and bacillus subtilis.
2. The microbial agent according to claim 1, wherein the viable count of the microbial agent is 10.0 hundred million/g or more.
3. The microbial inoculant according to claim 2, comprising the following components in parts by weight per 100 parts of microbial inoculant:
20-30 parts of bacillus thuringiensis, 10-15 parts of beauveria bassiana, 25-30 parts of bacillus subtilis, 20-35 parts of oligomeric maltose and 5-10 parts of an adsorbent.
4. The microbial inoculant according to claim 3, comprising the following components in parts by weight per 100 parts of microbial inoculant:
25 parts of bacillus thuringiensis, 13 parts of beauveria bassiana, 27 parts of bacillus subtilis, 25 parts of malto-oligosaccharide and 10 parts of adsorbent.
5. The microbial inoculant according to any one of claims 1 to 4, wherein the adsorbent is charcoal.
6. The microbial agent according to claim 5, wherein the microbial agent is a microbial agent obtained by mixing bacterial powder and malto-oligosaccharide.
7. The microbial inoculant according to claim 6, wherein the bacterial powder is prepared by:
(1) the preparation method of the bacillus thuringiensis fermentation liquor comprises the following steps: inoculating Bacillus thuringiensis seed liquid into a fermentation culture medium according to the inoculation amount of 3%, controlling the fermentation temperature to be 28 +/-0.2 ℃, and when the viable count in the fermentation liquid reaches 1 multiplied by 1010-2×1010Stopping fermentation when cfu/g;
(2) the preparation method of the beauveria bassiana fermentation liquor comprises the following steps: inoculating the Beauveria bassiana seed liquid into a fermentation culture medium according to the inoculation amount of 5%, controlling the fermentation temperature to be 26 +/-0.2 ℃, and when the viable count in the fermentation liquid reaches 1 multiplied by 1010-2×1010Stopping fermentation when cfu/g;
(3) preparation method of bacillus subtilis fermentation liquorComprises the following steps: inoculating the Bacillus subtilis seed solution into a fermentation culture medium according to the inoculation amount of 7%, controlling the fermentation temperature to be 35 +/-0.2 ℃, and when the viable count in the fermentation liquid reaches 1 multiplied by 1010-2×1010Stopping fermentation when cfu/g;
(4) and (3) mixing the fermentation liquors obtained in the steps (1), (2) and (3), adding an adsorbent into the mixture, stirring, standing for 24-36h, and spray-drying to obtain the bacterial powder.
8. The microbial inoculant according to claim 7, wherein the composition of the fermentation medium is as follows:
20-30g of corn starch, 15-20g of bean cake powder, 5-6g of yeast powder, 0.5-0.7g of magnesium sulfate, 0.2-0.4g of calcium chloride, 0.05-0.1g of manganese sulfate, 0.4-0.5g of monopotassium phosphate, 0.07-0.1g of copper sulfate and 1000ml of distilled water, and sterilizing at 121 ℃ for 30 min.
9. The microbial agent as claimed in any one of claims 1 to 8, which is used for controlling crop black mold.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111547645.3A CN114276956A (en) | 2021-12-16 | 2021-12-16 | Microbial agent and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111547645.3A CN114276956A (en) | 2021-12-16 | 2021-12-16 | Microbial agent and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114276956A true CN114276956A (en) | 2022-04-05 |
Family
ID=80872722
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111547645.3A Pending CN114276956A (en) | 2021-12-16 | 2021-12-16 | Microbial agent and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114276956A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115093294A (en) * | 2022-06-16 | 2022-09-23 | 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) | Microbial pesticide preparation for plant protection and growth regulation |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998021965A1 (en) * | 1996-11-18 | 1998-05-28 | Agraquest, Inc. | A novel strain of bacillus for controlling plant disease |
| CN110074141A (en) * | 2019-05-31 | 2019-08-02 | 昆明理工大学 | A kind of biocontrol agent and preparation method thereof for preventing and treating cacac moth |
| CN112493254A (en) * | 2020-12-04 | 2021-03-16 | 中诚国联（河南）生物科技有限公司 | Insecticidal organic-inorganic compound microbial agent and preparation method thereof |
| US20210100252A1 (en) * | 2018-05-08 | 2021-04-08 | Locus Agriculture Ip Company, Llc | Microbe-based products for enhancing plant root and immune health |
-
2021
- 2021-12-16 CN CN202111547645.3A patent/CN114276956A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998021965A1 (en) * | 1996-11-18 | 1998-05-28 | Agraquest, Inc. | A novel strain of bacillus for controlling plant disease |
| US20210100252A1 (en) * | 2018-05-08 | 2021-04-08 | Locus Agriculture Ip Company, Llc | Microbe-based products for enhancing plant root and immune health |
| CN110074141A (en) * | 2019-05-31 | 2019-08-02 | 昆明理工大学 | A kind of biocontrol agent and preparation method thereof for preventing and treating cacac moth |
| CN112493254A (en) * | 2020-12-04 | 2021-03-16 | 中诚国联（河南）生物科技有限公司 | Insecticidal organic-inorganic compound microbial agent and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 朱妍;刘姣;杜春梅;: "芽孢杆菌生物防治植物病害研究进展", 安徽农业科学, vol. 40, no. 34, pages 16635 - 16658 * |
| 李英等: "一株分离自感病桑天牛的高毒力球孢白僵菌菌株对 6 种植物病原真菌的拮抗作用研究", 蚕业科学, vol. 44, no. 1, pages 0025 - 0031 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115093294A (en) * | 2022-06-16 | 2022-09-23 | 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) | Microbial pesticide preparation for plant protection and growth regulation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN115700239A (en) | Fertilizer synergist and preparation method thereof | |
| CN108130301B (en) | Special compound microbial agent for ginger and preparation method and application thereof | |
| CN114276956A (en) | Microbial agent and application thereof | |
| CN110591937A (en) | Antagonistic actinomycetes and biological organic fertilizer for preventing and controlling tomato bacterial wilt, method and application | |
| CN104988094A (en) | Method for manufacturing quinclorac solid degrading inoculant | |
| CN109136144B (en) | Special compound biological agent for ginseng and production and preparation method thereof | |
| CN111499456A (en) | Fertilizer synergist containing chitosan oligosaccharide and preparation method thereof | |
| CN116410902A (en) | Biological microbial agent for preventing and treating root rot of astragalus and preparation method thereof | |
| CN113402327B (en) | Special microbial compound fertilizer for vegetables and preparation method thereof | |
| CN113293112B (en) | Composite microbial soil remediation agent and preparation method thereof | |
| CN116375519A (en) | Biological protective agent for rhizobium sojae as well as preparation method and application thereof | |
| CN114989834A (en) | Activating growth-promoting bacillus subtilis soil conditioner and preparation method thereof | |
| CN113881605A (en) | Compound microbial agent for preventing potato scab and preparation method thereof | |
| CN114350570A (en) | Liquid dosage form preparation method of rhizobium inoculant | |
| CN113583917A (en) | Compound microbial agent for ecological restoration and preparation method thereof | |
| CN113773125A (en) | Agricultural microbial agent and preparation method thereof | |
| CN112592241A9 (en) | Composite biological organic fertilizer with water retention function and preparation method thereof | |
| CN110683911A (en) | Biochar-based aflatoxin prevention and control slow-release fertilizer and preparation method thereof | |
| CN113881598B (en) | Antibacterial and detoxifying composite biocontrol microbial inoculum for relieving continuous cropping of watermelons and application thereof | |
| CN115820513B (en) | Microbial agent for preventing and treating soil-borne diseases and preparation method thereof | |
| CN110656072B (en) | Application of bacillus BCJB01 and BMJBN02 in strawberry production | |
| CN117106650A (en) | Agricultural compound microbial agent | |
| CN106922738A (en) | The method that biocontrol bacterial strain bacillus amyloliquefaciens Bam22 is used for increasing yield of water melons | |
| CN113979803A (en) | Production method of bio-control urea containing bacillus subtilis | |
| CN115584265A (en) | Organic source soil conditioner suitable for high-phosphorus high-cadmium soil and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220405 |