CN114272429A - Fetal bovine dermal matrix dressing and preparation method thereof - Google Patents
Fetal bovine dermal matrix dressing and preparation method thereof Download PDFInfo
- Publication number
- CN114272429A CN114272429A CN202111540744.9A CN202111540744A CN114272429A CN 114272429 A CN114272429 A CN 114272429A CN 202111540744 A CN202111540744 A CN 202111540744A CN 114272429 A CN114272429 A CN 114272429A
- Authority
- CN
- China
- Prior art keywords
- fetal bovine
- rotating
- minutes
- rotary drum
- ultrapure water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000283690 Bos taurus Species 0.000 title claims abstract description 58
- 230000001605 fetal effect Effects 0.000 title claims abstract description 58
- 239000011159 matrix material Substances 0.000 title claims abstract description 21
- 230000002500 effect on skin Effects 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 210000004207 dermis Anatomy 0.000 claims abstract description 39
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 17
- 230000004048 modification Effects 0.000 claims abstract description 15
- 238000012986 modification Methods 0.000 claims abstract description 15
- 238000004108 freeze drying Methods 0.000 claims abstract description 14
- 238000004140 cleaning Methods 0.000 claims abstract description 12
- 238000004806 packaging method and process Methods 0.000 claims abstract description 10
- 238000002791 soaking Methods 0.000 claims abstract description 10
- 238000004132 cross linking Methods 0.000 claims abstract description 9
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 229920001661 Chitosan Polymers 0.000 claims abstract description 8
- 150000003242 quaternary ammonium salts Chemical class 0.000 claims abstract description 8
- 229920000858 Cyclodextrin Polymers 0.000 claims abstract description 7
- 239000001116 FEMA 4028 Substances 0.000 claims abstract description 7
- 229960004853 betadex Drugs 0.000 claims abstract description 7
- 229940111630 tea tree oil Drugs 0.000 claims abstract description 7
- 239000010677 tea tree oil Substances 0.000 claims abstract description 7
- 230000001678 irradiating effect Effects 0.000 claims abstract description 4
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 26
- 239000012498 ultrapure water Substances 0.000 claims description 26
- 210000003491 skin Anatomy 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 20
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 10
- 230000018044 dehydration Effects 0.000 claims description 9
- 238000006297 dehydration reaction Methods 0.000 claims description 9
- 238000007599 discharging Methods 0.000 claims description 8
- 238000000465 moulding Methods 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 7
- 239000011265 semifinished product Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 238000012856 packing Methods 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical group [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims description 5
- 235000019270 ammonium chloride Nutrition 0.000 claims description 5
- 238000005520 cutting process Methods 0.000 claims description 5
- 239000012467 final product Substances 0.000 claims description 5
- 230000005251 gamma ray Effects 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 239000008055 phosphate buffer solution Substances 0.000 claims description 5
- 239000002985 plastic film Substances 0.000 claims description 5
- 229920006255 plastic film Polymers 0.000 claims description 5
- 238000010008 shearing Methods 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 238000012858 packaging process Methods 0.000 claims description 2
- 239000000047 product Substances 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 230000003385 bacteriostatic effect Effects 0.000 abstract description 4
- 230000035876 healing Effects 0.000 abstract description 4
- 230000002045 lasting effect Effects 0.000 abstract description 3
- 238000009740 moulding (composite fabrication) Methods 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 241001465754 Metazoa Species 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 230000015556 catabolic process Effects 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000010985 leather Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 239000013077 target material Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- ZNZYKNKBJPZETN-WELNAUFTSA-N Dialdehyde 11678 Chemical compound N1C2=CC=CC=C2C2=C1[C@H](C[C@H](/C(=C/O)C(=O)OC)[C@@H](C=C)C=O)NCC2 ZNZYKNKBJPZETN-WELNAUFTSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 210000000416 exudates and transudate Anatomy 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Abstract
The invention provides a fetal bovine dermal matrix dressing and a preparation method thereof, which comprises the steps of taking purified fetal bovine dermis as a raw material, putting the purified fetal bovine dermis into an ultrasonic rotary drum under the condition of ultrasonic waves, carrying out cross-linking modification on the purified fetal bovine dermis by using oxidized chitosan quaternary ammonium salt after soaking, and then adding dialdehyde-beta-cyclodextrin and tea tree oil for carrying out antibacterial modification. And finally, repeatedly cleaning, centrifugally dewatering, freeze-drying, forming, packaging and irradiating to obtain the fetal bovine dermal matrix dressing with the lasting antibacterial and bacteriostatic functions. The fetal bovine dermal matrix dressing prepared by the invention has the characteristics of good biocompatibility, excellent physical and mechanical properties and durable antibacterial and bacteriostatic properties, has the function of promoting healing, and is an ideal high-end dressing.
Description
Technical Field
The invention relates to the technical field of medical dressings, in particular to a fetal bovine dermal matrix dressing and a preparation method thereof.
Background
The medical dressing is a medical material used for covering the wound surface. The wound surface needs to be covered by a proper covering material in the healing process, and the wound surface can replace the damaged skin to obtain a certain protection effect in the healing process of the wound so as to avoid or control infection. The wound surface microenvironment refers to the humidity, temperature, pH value of acid and alkali and low oxygen tension of the wound surface, so that how to create an ideal microenvironment for the wound is the key to select a proper dressing. An ideal dressing should have the following characteristics: maintaining the wound at a stable temperature and humidity to absorb excess exudate; the paint has good permeability; preventing microorganisms, harmful particles and other harmful substances from polluting the wound; the dressing can be removed without damaging the wound and the like.
The animal dressing comprises allogenic/allogenic skin, animal skin and the like, and is mainly used for treating various skin defects including burn. The animal skin in the prior art is mainly pig skin, the source of the animal skin is wide, the components and the structure of the animal skin are very similar to those of human skin, but the existing pig skin dressing has poor mechanical property, is easy to delaminate, has high immunogenicity, is easy to be polluted by pathogenic microorganisms, is easy to be infected by bacteria when being used as a dressing, and limits the clinical application of the dressing. Therefore, it is desired to provide a substrate dressing having stable performance and excellent antibacterial properties.
Disclosure of Invention
The invention aims to provide a fetal bovine dermal matrix dressing and a preparation method thereof. The technical effects that can be produced by the preferred technical scheme in the technical schemes provided by the invention are described in detail in the following.
In order to achieve the purpose, the invention provides the following technical scheme:
the invention provides a preparation method of a fetal bovine dermal matrix dressing, which comprises the following steps:
(1) preparing raw materials: weighing a certain amount of purified fetal bovine dermis;
(2) soaking: placing the weighed purified fetal bovine dermis in an ultrasonic rotary drum, adding ultrapure water, rotating, soaking for 60-180 minutes, rotating, and draining;
(3) crosslinking modification: adding ultrapure water into the ultrasonic rotary drum, adjusting the temperature in the rotary drum to 25-40 ℃, adding 1-8% of oxidized chitosan quaternary ammonium salt, rotating for 60-180 minutes, and discharging liquid when the shrinkage temperature of the skin sheet reaches above 70 ℃;
(4) cleaning: adding ultrapure water into the ultrasonic rotary drum in the step (3), rotating, and draining;
(5) and (3) antibacterial modification: adding 50% of phosphate buffer solution with the pH value of 7.4 and 10-80% of dialdehyde-beta-cyclodextrin into the ultrasonic rotary drum in the step (4), rotating, adding 10-25% of tea tree oil dissolved by absolute ethyl alcohol, and rotating for 60-180 minutes; heating to enable the temperature in the rotary drum to reach 35-42 ℃, rotating for 10-20 hours, standing overnight, then rotating for 2-6 hours the next day, adding 3-7% of ammonium chloride, and discharging liquid after rotating;
(6) and sequentially cleaning, centrifugally dewatering, freeze-drying, molding, packaging and irradiating to obtain a finished product.
According to a preferred embodiment, in step (2), the method further comprises: placing 100 parts by weight of purified fetal bovine dermis in an ultrasonic rotary drum, adding ultrapure water with the weight of 200% of the weight of the fetal bovine dermis, rotating for 5 minutes, soaking for 60-180 minutes, rotating for 30 minutes, and draining.
According to a preferred embodiment, in step (4), the method further comprises: and (4) adding 200% of ultrapure water into the ultrasonic rotary drum in the step (3), rotating for 10 minutes, and draining.
According to a preferred embodiment, the washing process in step (6) comprises:
adding 200% of ultrapure water into the ultrasonic rotary drum in the step (5), and rotating for 10 minutes; and adding 200% of ultrapure water, rotating for 10 minutes, repeating the steps for 2-4 times, draining water, and taking out of the drum.
According to a preferred embodiment, the centrifugal dewatering process in step (6) comprises:
and (4) centrifugally dewatering the repeatedly cleaned semi-finished product on a centrifugal machine to ensure that the water content is 40-50%.
According to a preferred embodiment, the freeze-drying process in step (6) comprises:
placing the semi-finished product after centrifugal dehydration in a freeze dryer for freeze drying;
the forming process in the step (6) comprises the following steps:
cutting the freeze-dried skin sheets on a superclean workbench of a hundred-grade workshop by using a small shearing machine according to the specification requirement;
the packaging process in the step (6) comprises the following steps:
on the superclean bench of hundred grades of workshops, cut out fashioned skin piece and pack as required, the packing divide into inner packing and extranal packing: the inner package is divided into an inner layer and an outer layer, the inner layer is a plastic film, and the outer layer is a tin foil bag; the external package is a hard paper bag.
According to a preferred embodiment, the irradiation process in step (6) comprises:
sterilizing with gamma ray of 10.0-30.0KGy/h60Co to obtain the final product.
The invention also provides the fetal bovine dermal matrix dressing which is prepared according to the preparation method.
The fetal bovine dermal matrix dressing made in accordance with the above invention should meet the key performance requirements in table 1 below:
TABLE 1 Key Properties of fetal bovine dermal matrix dressings
Based on the technical scheme, the fetal bovine dermal matrix dressing and the preparation method thereof at least have the following technical effects:
the invention takes purified fetal bovine dermis as a raw material, the purified fetal bovine dermis is firstly placed into a rotary drum under the condition of ultrasonic waves, after being soaked, the purified fetal bovine dermis is subjected to cross-linking modification by using oxidized chitosan quaternary ammonium salt, not only is good physical and mechanical properties and degradation stability endowed to the purified fetal bovine dermis, but also good lasting antibacterial property is endowed to the purified fetal bovine dermis, and dialdehyde-beta-cyclodextrin and tea tree oil are added for carrying out antibacterial modification. And finally, repeatedly cleaning, centrifugally dewatering, freeze-drying, forming, packaging and irradiating to obtain the fetal bovine dermal matrix dressing with the lasting antibacterial and bacteriostatic functions. The dressing has the characteristics of good biocompatibility, excellent physical and mechanical properties and durable antibacterial and bacteriostatic properties, has the function of promoting healing, and is an ideal high-end dressing.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.
Example 1
This example 1 provides a method for preparing a fetal bovine dermal matrix dressing, including the following steps:
(1) preparing raw materials: and (3) taking the purified fetal bovine dermis, accurately weighing the fetal bovine dermis, and taking the fetal bovine dermis as a material counting basis in the following steps.
(2) Soaking: in an ultrasonic rotating drum. Firstly, 100 parts by weight of purified fetal bovine dermis is placed in an ultrasonic rotary drum, ultrapure water with the weight of 200% of the weight of the fetal bovine dermis is added, the fetal bovine dermis is rotated for 5 minutes, soaked for 60 minutes, rotated for 30 minutes, and drained.
(3) Crosslinking modification: adding 80% of ultrapure water into the ultrasonic rotary drum, adjusting the temperature in the rotary drum to 25 ℃, adding 4% of oxidized chitosan quaternary ammonium salt, rotating for 60 minutes, detecting the shrinkage temperature of the skin piece, requiring the shrinkage temperature to reach more than 70 ℃, and discharging liquid after the shrinkage temperature of the skin piece meets the requirement.
(4) Cleaning: 200% ultrapure water was added thereto, and the mixture was rotated for 10 minutes and then drained.
(5) And (3) antibacterial modification: adding 50% phosphate buffer solution with pH of 7.4, adding 10% dialdehyde-beta-cyclodextrin, rotating for 10 min, adding 10% tea tree oil dissolved completely with anhydrous ethanol, rotating for 120 min, heating to 35 deg.C, rotating for 10 hr, and standing overnight. The next day, rotate for 2 hours, add 3% ammonium chloride, rotate for 10 minutes, drain.
(6) Cleaning: adding 200% ultrapure water, rotating for 10 min, repeating the steps for 2 times, draining, and taking out of the drum.
(7) And (3) centrifugal dehydration: centrifuging and dewatering in a centrifuge with water content of 40-50%.
(8) And (3) freeze drying: and (4) placing the semi-finished product subjected to centrifugal dehydration in a freeze dryer for freeze drying.
(9) Molding: and (4) cutting the freeze-dried skin sheets on a superclean workbench of a hundred-grade workshop by using a small shearing machine according to the specification requirement for molding.
(10) Packaging: and packaging the cut and formed leather sheets on a superclean workbench of a hundred-grade workshop according to requirements. The package is divided into an inner package and an outer package: the inner package is divided into an inner layer and an outer layer, the inner layer is a plastic film, and the outer layer is a tin foil bag; the external package is a hard paper bag.
(11) Irradiation: sterilizing with gamma ray of 10KGy/h60Co to obtain the final product.
Example 2
This example 2 provides a method for preparing a fetal bovine dermal matrix dressing, including the following steps:
(1) preparing raw materials: and (3) taking the purified fetal bovine dermis, accurately weighing the fetal bovine dermis, and taking the fetal bovine dermis as a material counting basis in the following steps.
(2) Soaking: in an ultrasonic rotating drum. Firstly, 100 parts by weight of purified fetal bovine dermis is placed in an ultrasonic rotary drum, ultrapure water with the weight of 200% of the weight of the fetal bovine dermis is added, the fetal bovine dermis is rotated for 5 minutes, soaked for 120 minutes, rotated for 30 minutes, and drained.
(3) Crosslinking modification: adding 140% of ultrapure water into the ultrasonic rotary drum, adjusting the temperature in the rotary drum to 33 ℃, adding 6% of oxidized chitosan quaternary ammonium salt, rotating for 120 minutes, detecting the shrinkage temperature of the skin piece, and requiring the shrinkage temperature to reach more than 70 ℃. And discharging liquid after the shrinkage temperature meets the requirement.
(4) Cleaning: 200% ultrapure water was added thereto, and the mixture was rotated for 10 minutes and then drained.
(5) And (3) antibacterial modification: adding 50% phosphate buffer solution with pH 7.4, adding 45% dialdehyde-beta-cyclodextrin, rotating for 10 min, adding 18% tea tree oil dissolved completely with anhydrous ethanol, rotating for 120 min, heating to 39 deg.C, rotating for 15 hr, standing overnight, rotating for 4 hr the next day, adding 5% ammonium chloride, and rotating for 10 min. And (6) draining.
(6) Cleaning: adding 200% ultrapure water, rotating for 10 min, repeating the steps for 3 times, draining, and taking out of the drum.
(7) And (3) centrifugal dehydration: centrifuging and dewatering in a centrifuge with water content of 40-50%.
(8) And (3) freeze drying: and (4) placing the semi-finished product subjected to centrifugal dehydration in a freeze dryer for freeze drying.
(9) Molding: and (4) cutting the freeze-dried skin sheets on a superclean workbench of a hundred-grade workshop by using a small shearing machine according to the specification requirement for molding.
(10) Packaging: and packaging the cut and formed leather sheets on a superclean workbench of a hundred-grade workshop according to requirements. The package is divided into an inner package and an outer package: the inner package is divided into an inner layer and an outer layer, the inner layer is a plastic film, and the outer layer is a tin foil bag; the external package is a hard paper bag.
(11) Irradiation: sterilizing with gamma ray of 20KGy/h60Co to obtain the final product.
Example 3
This example 3 provides a method for preparing a fetal bovine dermal matrix dressing, including the following steps:
(1) preparing raw materials: and (3) taking the purified fetal bovine dermis, accurately weighing the fetal bovine dermis, and taking the fetal bovine dermis as a material counting basis in the following steps.
(2) Soaking: in an ultrasonic rotating drum. Firstly, 100 parts by weight of purified fetal bovine dermis is placed in an ultrasonic rotary drum, ultrapure water with the weight of 200% of the weight of the fetal bovine dermis is added, the fetal bovine dermis is rotated for 5 minutes, soaked for 180 minutes, rotated for 30 minutes, and drained.
(3) Crosslinking modification: adding 200% of ultrapure water into the ultrasonic rotary drum, adding 8% of oxidized chitosan quaternary ammonium salt when the temperature in the rotary drum reaches 40 ℃, rotating for 180 minutes, detecting the shrinkage temperature of the skin piece, requiring the shrinkage temperature to reach more than 70 ℃, and discharging liquid after the shrinkage temperature meets the requirement.
(4) Cleaning: 200% ultrapure water was added thereto, and the mixture was rotated for 10 minutes and then drained.
(5) And (3) antibacterial modification: adding 50% phosphate buffer solution with pH 7.4, adding 80% dialdehyde-beta-cyclodextrin, rotating for 10 min, adding 25% tea tree oil dissolved completely with anhydrous ethanol, rotating for 180 min, heating to 42 deg.C, rotating for 20 hr, standing overnight, rotating for 6 hr the next day, adding 7% ammonium chloride, rotating for 10 min, and discharging.
(6) Cleaning: adding 200% ultrapure water, rotating for 10 min, repeating the steps for 4 times, draining, and taking out of the drum.
(7) And (3) centrifugal dehydration: centrifuging and dewatering in a centrifuge with water content of 40-50%.
(8) And (3) freeze drying: and (4) placing the semi-finished product subjected to centrifugal dehydration in a freeze dryer for freeze drying.
(9) Molding: and (4) cutting the freeze-dried skin sheets on a superclean workbench of a hundred-grade workshop by using a small shearing machine according to the specification requirement for molding.
(10) Packaging: and packaging the cut and formed leather sheets on a superclean workbench of a hundred-grade workshop according to requirements. The package is divided into an inner package and an outer package: the inner package is divided into an inner layer and an outer layer, the inner layer is a plastic film, and the outer layer is a tin foil bag; the external package is a hard paper bag.
(11) Irradiation: sterilizing with gamma ray of 30.0KGy/h60Co to obtain the final product.
The results of testing the key performance indicators of the fetal bovine dermal matrix dressings prepared in examples 1 to 3 are shown in table 2 below:
TABLE 2 Key Performance index test results
The purified fetal bovine dermis adopted by the preparation method has rich raw material sources, low price, easy obtainment and reliable quality, and the durable antibacterial fetal bovine dermis matrix dressing prepared by the method not only endows the target material with good physical and mechanical properties and degradation stability, but also endows the target material with good durable antibacterial performance because the purified fetal bovine dermis is subjected to crosslinking modification by adopting the oxidized chitosan quaternary ammonium salt. Particularly, the purified fetal bovine dermis is modified by dialdehyde with both crosslinking function and inclusion capacity, so that the durable antibacterial fetal bovine dermis matrix dressing with good biocompatibility, degradation stability, physical and mechanical properties and excellent temperature-sensitive slow-release antibacterial property is prepared. In addition, the raw materials, equipment and key materials used by the invention are all made from China.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (8)
1. A preparation method of a fetal bovine dermal matrix dressing is characterized by comprising the following steps:
(1) preparing raw materials: weighing a certain amount of purified fetal bovine dermis;
(2) soaking: placing the weighed purified fetal bovine dermis in an ultrasonic rotary drum, adding ultrapure water, rotating, soaking for 60-180 minutes, rotating, and draining;
(3) crosslinking modification: adding ultrapure water into the ultrasonic rotary drum, adjusting the temperature in the rotary drum to 25-40 ℃, adding 1-8% of oxidized chitosan quaternary ammonium salt, rotating for 60-180 minutes, and discharging liquid when the shrinkage temperature of the skin sheet reaches above 70 ℃;
(4) cleaning: adding ultrapure water into the ultrasonic rotary drum in the step (3), rotating, and draining;
(5) and (3) antibacterial modification: adding 50% of phosphate buffer solution with the pH value of 7.4 and 10-80% of dialdehyde-beta-cyclodextrin into the ultrasonic rotary drum in the step (4), rotating, adding 10-25% of tea tree oil dissolved by absolute ethyl alcohol, and rotating for 60-180 minutes; heating to enable the temperature in the rotary drum to reach 35-42 ℃, rotating for 10-20 hours, standing overnight, then rotating for 2-6 hours the next day, adding 3-7% of ammonium chloride, and discharging liquid after rotating;
(6) and sequentially cleaning, centrifugally dewatering, freeze-drying, molding, packaging and irradiating to obtain a finished product.
2. The method according to claim 1, wherein in the step (2), further comprising: placing 100 parts by weight of purified fetal bovine dermis in an ultrasonic rotary drum, adding ultrapure water with the weight of 200% of the weight of the fetal bovine dermis, rotating for 5 minutes, soaking for 60-180 minutes, rotating for 30 minutes, and draining.
3. The method according to claim 1, wherein in the step (4), further comprising: and (4) adding 200% of ultrapure water into the ultrasonic rotary drum in the step (3), rotating for 10 minutes, and draining.
4. The method according to claim 1, wherein the washing process in the step (6) comprises:
adding 200% of ultrapure water into the ultrasonic rotary drum in the step (5), and rotating for 10 minutes; and adding 200% of ultrapure water, rotating for 10 minutes, repeating the steps for 2-4 times, draining water, and taking out of the drum.
5. The method according to claim 1, wherein the centrifugal dehydration process in the step (6) comprises:
and (4) centrifugally dewatering the repeatedly cleaned semi-finished product on a centrifugal machine to ensure that the water content is 40-50%.
6. The method according to claim 1, wherein the freeze-drying process in the step (6) comprises:
placing the semi-finished product after centrifugal dehydration in a freeze dryer for freeze drying;
the forming process in the step (6) comprises the following steps:
cutting the freeze-dried skin sheets on a superclean workbench of a hundred-grade workshop by using a small shearing machine according to the specification requirement;
the packaging process in the step (6) comprises the following steps:
on the superclean bench of hundred grades of workshops, cut out fashioned skin piece and pack as required, the packing divide into inner packing and extranal packing: the inner package is divided into an inner layer and an outer layer, the inner layer is a plastic film, and the outer layer is a tin foil bag; the external package is a hard paper bag.
7. The method according to claim 1, wherein the irradiation process in the step (6) comprises:
sterilizing with gamma ray of 10.0-30.0KGy/h60Co to obtain the final product.
8. A fetal bovine dermal matrix dressing prepared according to the preparation method of any one of claims 1 to 7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111540744.9A CN114272429A (en) | 2021-12-16 | 2021-12-16 | Fetal bovine dermal matrix dressing and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111540744.9A CN114272429A (en) | 2021-12-16 | 2021-12-16 | Fetal bovine dermal matrix dressing and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114272429A true CN114272429A (en) | 2022-04-05 |
Family
ID=80872466
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111540744.9A Pending CN114272429A (en) | 2021-12-16 | 2021-12-16 | Fetal bovine dermal matrix dressing and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114272429A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104013995A (en) * | 2014-06-26 | 2014-09-03 | 四川大学 | Oxidation chitosan graft modified porcine dermal collagen micro-nano fiber membrane and preparation method thereof |
| CN106215239A (en) * | 2016-08-31 | 2016-12-14 | 四川大学 | A kind of preparation method of crosslinked antimicrobial type acellular matrix material |
| CN106215222A (en) * | 2016-07-07 | 2016-12-14 | 四川大学 | A kind of aoxidize oligochitosan crosslinked with collagen and method that in-situ preparation nanometer silver prepares antimicrobial form collagen |
| CN106310352A (en) * | 2016-11-01 | 2017-01-11 | 广东泰宝医疗科技股份有限公司 | Preparation method of antibacterial acellular dermal matrix dressing material |
| CN106366333A (en) * | 2016-08-31 | 2017-02-01 | 四川大学 | Oxidized chitosan quaternary ammonium salt and collagen crosslinking method |
| WO2018199698A1 (en) * | 2017-04-28 | 2018-11-01 | (주)시지바이오 | Composition comprising fibrous acellular dermal matrix and biocompatible polymer, and method for producing same |
| CN113274541A (en) * | 2021-05-10 | 2021-08-20 | 四川大学 | Antibacterial collagen-based medical material and preparation method thereof |
-
2021
- 2021-12-16 CN CN202111540744.9A patent/CN114272429A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104013995A (en) * | 2014-06-26 | 2014-09-03 | 四川大学 | Oxidation chitosan graft modified porcine dermal collagen micro-nano fiber membrane and preparation method thereof |
| CN106215222A (en) * | 2016-07-07 | 2016-12-14 | 四川大学 | A kind of aoxidize oligochitosan crosslinked with collagen and method that in-situ preparation nanometer silver prepares antimicrobial form collagen |
| CN106215239A (en) * | 2016-08-31 | 2016-12-14 | 四川大学 | A kind of preparation method of crosslinked antimicrobial type acellular matrix material |
| CN106366333A (en) * | 2016-08-31 | 2017-02-01 | 四川大学 | Oxidized chitosan quaternary ammonium salt and collagen crosslinking method |
| CN106310352A (en) * | 2016-11-01 | 2017-01-11 | 广东泰宝医疗科技股份有限公司 | Preparation method of antibacterial acellular dermal matrix dressing material |
| WO2018199698A1 (en) * | 2017-04-28 | 2018-11-01 | (주)시지바이오 | Composition comprising fibrous acellular dermal matrix and biocompatible polymer, and method for producing same |
| CN113274541A (en) * | 2021-05-10 | 2021-08-20 | 四川大学 | Antibacterial collagen-based medical material and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lu et al. | Healing of skin wounds with a chitosan–gelatin sponge loaded with tannins and platelet-rich plasma | |
| Siritientong et al. | The effect of sterilization methods on the physical properties of silk sericin scaffolds | |
| CN108066805B (en) | Epsilon-polylysine bionic antibacterial film and preparation and application thereof | |
| CN103463668B (en) | A kind of preparation method of silk fibroin-calcium alginate biological wound porous dressing | |
| CN104013995B (en) | Oxidation chitosan graft modification pig dermis collagen micro-nano fiber film and preparation method thereof | |
| Rahman et al. | Preparation and characterization of porous scaffold composite films by blending chitosan and gelatin solutions for skin tissue engineering | |
| US20050287193A1 (en) | Wound dressing made of chitin and alginate and method for forming the same | |
| Aramwit et al. | A green salt‐leaching technique to produce sericin/PVA/glycerin scaffolds with distinguished characteristics for wound‐dressing applications | |
| CN106806934B (en) | Dressing with antibacterial repair function and preparation method thereof | |
| CN107551312A (en) | A kind of cotton-shaped collagen hemostasis fiber and preparation method thereof | |
| CN111346256A (en) | Freeze-drying forming optimization method of medical hemostatic healing sponge | |
| CN107789658A (en) | A kind of preparation method of crosslinking electron beam irradiation composite high-molecular aerogel dressing | |
| CN106421868A (en) | Chitosan quaternary ammonium salt porcine acellular dermal matrix dressing material and preparation method thereof | |
| CN106344954A (en) | Bio-antimicrobial bacterial cellulose dressing and preparation method thereof | |
| CN108245700A (en) | A kind of hydroxypropyl methyl cellulose chitosan film dressing and preparation method thereof | |
| CN106822980B (en) | Plasticized nano bacterial cellulose functional dressing and preparation method thereof | |
| Guamba et al. | Cellulose-based hydrogels towards an antibacterial wound dressing | |
| KR20130086770A (en) | Manifacturing method of dried bio-cellulose | |
| CN114272429A (en) | Fetal bovine dermal matrix dressing and preparation method thereof | |
| CN113750285A (en) | Composite aerogel dressing and preparation method thereof | |
| CN109464697A (en) | A kind of polyvinyl alcohol/chitosan/graphene combine dressing and preparation method thereof | |
| JP4486304B2 (en) | Microbial cellulosic wound dressings for the treatment of chronic wounds | |
| CN110507848B (en) | Enzyme-loaded bacterial cellulose-based composite antibacterial hydrogel dressing and preparation method thereof | |
| WO2011100935A1 (en) | Dry material of hydrogel for wound dressing and its method of preparation | |
| KR102271980B1 (en) | Collagen-arginate wound dressing and method of preparing the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20221013 Address after: Floor 8, Building 1, No. 670 Haifa Road, Chengdu Cross Strait Science and Technology Industry Development Park, Wenjiang District, Chengdu, 610000, Sichuan Applicant after: Chengdu Kelejin Biotechnology Co.,Ltd. Address before: 610000 room 1106, unit 2, No. 699, middle section of Yizhou Avenue, high tech Zone, Chengdu, China (Sichuan) pilot Free Trade Zone, Chengdu, Sichuan Applicant before: Chengdu handing New Material Technology Co.,Ltd. |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220405 |