CN114271467A - A method for preparing gorgeous leaf extract and the prepared gorgeous leaf extract - Google Patents
A method for preparing gorgeous leaf extract and the prepared gorgeous leaf extract Download PDFInfo
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- CN114271467A CN114271467A CN202111651755.4A CN202111651755A CN114271467A CN 114271467 A CN114271467 A CN 114271467A CN 202111651755 A CN202111651755 A CN 202111651755A CN 114271467 A CN114271467 A CN 114271467A
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Abstract
The invention discloses a preparation method of a gorgeous leaf extract, which comprises the following steps: firstly, enzymolysis of cellulose: soaking fresh gorgeous leaves or gorgeous powder, and performing enzymolysis treatment by using biological enzyme to obtain enzymolysis liquid and enzymolysis residues; (II) alcohol solution extraction: soaking the enzymolysis residues in an alcohol solution for extraction to obtain a gorgeous extracting solution; and (III) concentrating to remove chlorophyll: concentrating the obtained extract, removing chlorophyll by centrifugation during concentration, and obtaining the extracts of the gorgeous leaves in different states according to different applications. The invention processes the gorgeous leaves and the gorgeous powder, keeps the original flavor while greatly reducing chlorophyll, ensures that the characteristic fragrance is more outstanding, the flavor is better, increases the shelf life of the gorgeous extract or the concentrated solution, can improve the smoke of the cigarette when being used as a tobacco additive, and has the synergistic effect on the intrinsic fragrance of the tobacco. The separated chlorophyll can be further purified or made into sodium copper chlorophyllin products.
Description
Technical Field
The invention relates to the field of perfume manufacturing, in particular to a method for preparing a gorgeous leaf extract.
Background
Gorgeous leaves, also known as variegated leaves, known under the scientific name of marsupium cordifolia, and latin under the scientific name of Pandanus amarylius roxb. The planting is spread to countries such as Singapore, Malaysia, Srilanka, India, China, Thailand and the like, Hainan is a planting origin and a dominant production area of the leaf of Epimedium hybridum in China, and planting areas mainly comprise Wanning, Qinhai, delirium, tomb water, pavilion, Dingan and the like, wherein Wanning is used as a main distribution area and a traditional utilization area, the planting area is about 5000 mu, at present, abundant utilization culture of the leaf of Epimedium hybridum in south of China is available, the southern culture is mainly used as a carrier, and the national comprehensive utilization mode is mainly used in industries such as special catering, ornamental gardening, leisure travel and the like.
The multicolored fragrance mainly comprises 3-methyl-2 (5H) -furanone, neophytadiene, 2-acetyl-1-pyrroline, phytol, 2, 3-dihydrobenzofuran, 2, 4-di-tert-butylphenol, hydroxyacetone, ethyl palmitate, ethyl linoleate and the like, has rice fragrance and coconut fragrance, and is popular with people. However, the gorgeous chlorophyll contains a large amount of chlorophyll, so that the aqua, tincture and pulp of the medicinal preparation are often strongly smelled by grass, and the chlorophyll is extremely easy to oxidize and deteriorate and is not beneficial to preservation and application. In cigarettes, multicolored aroma components such as 3-methyl-2 (5H) -furanone, neophytadiene, 2-acetyl-1-pyrroline and the like can be coordinated and enhanced, but due to the high chlorophyll content, smoke is stimulated to be acerb, so that the multicolored extract is not used in cigarettes.
Therefore, there is a need for a method for reliably and stably producing a gorgeous extract which removes chlorophyll and retains the characteristic gorgeous leaves.
Disclosure of Invention
In order to overcome the above disadvantages of the prior art, the present invention is directed to a method for preparing a multicolored leaf extract or concentrate capable of being uniformly dried.
The technical scheme adopted by the invention for solving the technical problems is as follows:
a method for preparing a gorgeous leaf extract comprises the following steps:
firstly, enzymolysis of cellulose: soaking fresh gorgeous leaves or gorgeous powder, and performing enzymolysis treatment by using biological enzyme to obtain enzymolysis liquid and enzymolysis residues;
(II) alcohol solution extraction: soaking the enzymolysis residues in an alcohol solution for extraction to obtain a gorgeous extracting solution;
and (III) concentrating to remove chlorophyll: concentrating the obtained extract to remove chlorophyll separated out during concentration to obtain gorgeous leaf extract, which is in the form of concentrated liquid/paste/powder.
As a modification, the biological enzyme used in the step (one) comprises one or more of cellulase, cellobiase and levulose.
As a modified scheme, the feed-liquid ratio in the step (one) is 1:3-1: 20; the temperature of enzymolysis is within the range of 35-75 ℃.
As a modification, the enzyme is added in the step (one) in an amount of 0.1-2% of the substrate.
As an improvement scheme, one or more of alcohols with the alcohol solution type of C1-C4 are added in the step (II), and the concentration of the alcohol solution is 30-95%.
As a modification, the ratio of the alcohol solution added in the step (II) to the alcohol solution is in the range of 1:3-1: 20.
As an improved scheme, in the step (II), the enzymolysis residues are soaked in an alcohol solution for reflux extraction, and the alcohol extraction temperature range is 35-95 ℃.
As a modification, the gorgeous extract in the step (III) is subjected to vacuum concentration, and then chlorophyll is removed by using a centrifugal supernatant/filtration method, thereby obtaining gorgeous extract or gorgeous concentrate according to application requirements.
As a modification, in the step (III), the gorgeous extracting solution is added into the enzymatic hydrolysate obtained in the step (I) to be concentrated, the extraction rate of the gorgeous leaves is improved, and the gorgeous characteristic fragrance of the product is improved.
The gorgon leaf extract prepared as described above was used in the form of a concentrate/paste/powder. Wherein the obtained product can be used for cigarette, baking, beverage, cold drink, etc.; the obtained concentrated solution can be used for cigarette, beverage, cold drink, baking, etc.; the obtained product can be used for baking, cold drink, instant beverage, etc.
Compared with the prior art, the invention has the beneficial effects that:
the invention processes the gorgeous leaves and the gorgeous powder, keeps the original flavor while greatly reducing chlorophyll, ensures that the characteristic fragrance is more outstanding and the flavor is better, prolongs the shelf life of the gorgeous extract or the concentrated solution, and can enhance the original fragrance of the cigarette when being used as a tobacco additive. The extracted chlorophyll can be further purified or used for producing sodium copper chlorophyllin products.
Detailed Description
The invention will now be further illustrated with reference to the following examples:
the first embodiment is as follows:
a method for preparing a multicolored leaf concentrate comprises the following steps:
firstly, enzymolysis of cellulose: soaking 100g of fresh multicolored leaves or powder in 500g of water, adding 0.5g of cellulase, stirring at 45 deg.C for enzymolysis for 3 hr, and filtering to obtain enzymolysis solution and enzymolysis residue;
(II) alcohol solution extraction: soaking the enzymolysis residue in 800g 80% ethanol solution, heating and reflux extracting for 2 hr, filtering, and removing residue to obtain multicolored extractive solution;
and (III) concentrating to remove chlorophyll: concentrating the obtained gorgeous extract to 200g, centrifuging, and removing chlorophyll precipitated during concentration to obtain 195g of gorgeous leaf concentrate. Chlorophyll is stored in dark for another use.
The second embodiment:
a method for preparing a concentrated paste with gorgeous leaves comprises the following steps:
firstly, enzymolysis of cellulose: soaking 300g of fresh gorgeous leaves or gorgeous powder in 3000g of water, adding 6g of cellulase, stirring at 55 ℃ for enzymolysis for 5 hours, and filtering to obtain an enzymolysis solution and enzymolysis residues;
(II) alcohol solution extraction: soaking the enzymolysis residue in 6000g of 95% methanol solution, heating and reflux extracting for 6 hr, filtering, and removing residue to obtain multicolored extractive solution;
and (III) concentrating to remove chlorophyll: concentrating the obtained gorgeous extractive solution to 150g, centrifuging, removing chlorophyll precipitated during concentration, and concentrating to paste to obtain 47g of gorgeous concentrated paste. Chlorophyll is stored in dark for another use.
The third embodiment is as follows:
a method for preparing a gorgeous leaf extract comprises the following steps:
firstly, enzymolysis of cellulose: soaking 500g of fresh gorgeous leaves or gorgeous powder in 1500g of water, adding 0.5g of cellulase, stirring at 50 deg.C for enzymolysis for 4 hr, and filtering to obtain enzymolysis solution and enzymolysis residue;
(II) alcohol solution extraction: soaking the enzymolysis residues in 1500g 95% propanol solution, heating and reflux extracting for 3 hr, filtering, and removing residue to obtain multicolored extractive solution;
and (III) concentrating to remove chlorophyll: concentrating the obtained extract to 500g, centrifuging, removing chlorophyll precipitated during concentration, adding 250g cyclodextrin, dispersing with homogenizer, and spray drying to obtain 320g of gorgeous extract. Chlorophyll is stored in dark for another use.
The fourth embodiment is as follows:
a method for preparing a multicolored leaf concentrate comprises the following steps:
firstly, enzymolysis of cellulose: soaking 100g of fresh multicolored leaves or powder in 500g of water, adding 0.5g of cellulase, stirring at 45 deg.C for enzymolysis for 3 hr, filtering to obtain enzymolysis solution and enzymolysis residues, and deactivating enzyme to recover the enzymolysis solution;
(II) alcohol solution extraction: soaking the enzymolysis residue in 800g 80% ethanol solution, heating and reflux extracting for 2 hr, filtering, and removing residue to obtain multicolored extractive solution;
and (III) concentrating to remove chlorophyll: mixing the enzymolysis solution and the gorgeous extract, concentrating to 230g, centrifuging, and removing chlorophyll separated out during concentration to obtain 220g of gorgeous leaf concentrate. Chlorophyll is stored in dark for another use.
In summary, after reading the document of the present invention, those skilled in the art can make various other corresponding changes according to the technical scheme and technical concept of the present invention without creative mental labor, and different application scenarios all belong to the protection scope of the present invention.
Claims (10)
1. A method for preparing a multicolored leaf extract is provided,
the method comprises the following steps:
firstly, enzymolysis of cellulose: soaking fresh gorgeous leaves or gorgeous powder, and performing enzymolysis treatment by using biological enzyme to obtain enzymolysis liquid and enzymolysis residues;
(II) alcohol solution extraction: soaking the enzymolysis residues in an alcohol solution for extraction to obtain a gorgeous extracting solution;
and (III) concentrating to remove chlorophyll: concentrating the obtained extract to remove chlorophyll separated out during concentration to obtain gorgeous leaf extract.
2. The method of claim 1, wherein the biological enzyme used in step (a) comprises one or more of cellulase, cellobiase, and pectinase.
3. The method of claim 1, wherein the ratio of the materials in step (one) is 1:3-1:20, and the temperature of enzymolysis is in the range of 35-75 deg.C. The reaction time is 2-8 hours.
4. The method of claim 1, wherein the step (two) comprises adding one or more alcohols selected from the group consisting of C1-C4 in an alcohol solution concentration of 30-95%.
5. The method of preparing a diversity leaf extract according to claim 1, wherein the alcohol solution is added in the step (two) at a ratio of materials to liquids ranging from 1:3 to 1:20 for an extraction time of 1 to 6 hours.
6. The method of preparing a gorgon leaf extract as set forth in claim 1, wherein the enzymatic dregs are soaked in an alcohol solution and extracted under reflux in the step (two).
7. The method of preparing a gorgeous leaf extract according to claim 1, wherein the gorgeous leaf extract in the step (three) is vacuum-concentrated and chlorophyll is removed using a centrifugal supernatant/filtration method.
8. The method of preparing a multicolored leaf extract as claimed in claim 1, wherein the multicolored leaf extract obtained in the step (three) is in a concentrated liquid/paste/powder form; wherein the powder gorgeous leaf extract is added with adjuvants, and the adjuvants comprise one or more of cyclodextrin, modified starch, and maltodextrin.
9. The method of producing an extract from diversity leaves as claimed in claim 1, wherein in the step (iii), the extraction solution is added to the enzymatic solution obtained in the step (i) and concentrated together, thereby improving the extraction rate of diversity leaves and the characteristic of diversity.
10. A multicolored leaf extract prepared using the method of any of claims 1 to 9.
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| CN1034947A (en) * | 1988-02-12 | 1989-08-23 | 中国农业科学院茶叶研究所 | Preparation process for natural tea antioxiant |
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| CN104351724A (en) * | 2014-11-18 | 2015-02-18 | 深圳市深宝技术中心有限公司 | Production method of instant high-aroma pandan leaf powder |
| CN108617698A (en) * | 2018-06-05 | 2018-10-09 | 陈奥陶 | A kind of Sargassum horneri extract for promoting seed to sprout |
| CN110483532A (en) * | 2019-09-05 | 2019-11-22 | 卓士鸿 | The method that bunge pricklyash leaf extracts chlorophyll and aromatic oil |
| CN113350827A (en) * | 2021-07-05 | 2021-09-07 | 广州市名花香料有限公司 | Fissistigma Oldhamii extractive solution and its preparation method |
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2021
- 2021-12-30 CN CN202111651755.4A patent/CN114271467A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1034947A (en) * | 1988-02-12 | 1989-08-23 | 中国农业科学院茶叶研究所 | Preparation process for natural tea antioxiant |
| CN104083900A (en) * | 2014-07-15 | 2014-10-08 | 广州中大南沙科技创新产业园有限公司 | Method for extracting volatile components from Pandanus amaryllifolius Roxb leaves |
| CN104351724A (en) * | 2014-11-18 | 2015-02-18 | 深圳市深宝技术中心有限公司 | Production method of instant high-aroma pandan leaf powder |
| CN108617698A (en) * | 2018-06-05 | 2018-10-09 | 陈奥陶 | A kind of Sargassum horneri extract for promoting seed to sprout |
| CN110483532A (en) * | 2019-09-05 | 2019-11-22 | 卓士鸿 | The method that bunge pricklyash leaf extracts chlorophyll and aromatic oil |
| CN113350827A (en) * | 2021-07-05 | 2021-09-07 | 广州市名花香料有限公司 | Fissistigma Oldhamii extractive solution and its preparation method |
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| 林萍;: "竹叶提取叶绿素工艺", 中国林业产业, no. 10, pages 46 * |
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