CN114259444B - A black tiger fruit extract with whitening effect, its preparation method and application in preparing bath lotion with whitening effect - Google Patents
A black tiger fruit extract with whitening effect, its preparation method and application in preparing bath lotion with whitening effect Download PDFInfo
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Abstract
The invention discloses a black tiger fruit extract with a whitening effect, a preparation method thereof and application thereof in preparing shower gel with the whitening effect. The preparation method of the black tiger fruit extract with the whitening effect comprises the following steps: (1) Extracting dried black tiger fruit with organic solvent, concentrating and drying the organic solvent extract to obtain organic solvent extract; (2) suspending the organic solvent extract with water; extracting with ethyl acetate, extracting with n-butanol, concentrating n-butanol extractive solution, and drying to obtain n-butanol extract, namely the extract of herba Hedyotidis Diffusae with whitening effect. Research shows that the black tiger fruit extract prepared by the method has excellent whitening effect; therefore, the active ingredient of the active ingredient is further added into the bath foam, so that the prepared bath foam has the whitening effect.
Description
Technical Field
The invention relates to the technical field of natural medicinal chemistry, in particular to a black tiger fruit extract with a whitening effect, a preparation method thereof and application thereof in preparing bath cream with the whitening effect.
Background
Black tiger, the subject of which is Kadsura coccinea (lem.) a.c. smith), is a plant of the genus Kadsura. The provinces of Jiangxi, hunan, guangdong, hainan, guangxi, sichuan, guizhou and Yunnan are distributed in China. The root has the effects of promoting qi and blood circulation, relieving swelling and pain, and the like, and is commonly used for treating stomach diseases, rheumatalgia, traumatic blood stasis and pain, and gynaecology.
The black tiger fruit refers to black tiger fruits, contains abundant vitamin C, vitamin E, amino acids and various trace elements, and is a favorite wild fruit. However, there are few reports on whether or not the ingredients of black tiger fruit have whitening effect. Therefore, the black tiger fruit extract with the whitening effect is developed from the black tiger fruits and has important application value.
Disclosure of Invention
In order to overcome at least one technical problem in the prior art, the invention firstly provides a preparation method of a black tiger fruit extract with a whitening effect. Research shows that the black tiger fruit extract prepared by the method has excellent whitening effect.
The technical problems to be solved by the invention are realized by the following technical scheme:
a preparation method of black tiger fruit extract with whitening effect comprises the following steps:
(1) Extracting dried black tiger fruit with organic solvent, concentrating and drying the organic solvent extract to obtain organic solvent extract;
(2) Suspending the organic solvent extract with water; extracting with ethyl acetate, extracting with n-butanol, concentrating n-butanol extractive solution, and drying to obtain n-butanol extract, namely the extract of herba Hedyotidis Diffusae with whitening effect.
The dried black cutworm fruits refer to the dried black cutworm fruits.
Preferably, the organic solvent in step (1) is an aqueous propylene glycol solution.
In the invention, the selection of the organic solvent plays a decisive role in preparing the black tiger fruit extract with whitening effect, especially excellent whitening effect. The inventor surprisingly found that the n-butanol extract obtained by extracting dried black tiger fruits by adopting a propylene glycol aqueous solution and then n-butanol has excellent whitening effect in a large number of experimental processes; the whitening effect is far greater than that of the black tiger fruit extract prepared by adopting common solvents such as ethanol, water and the like.
Preferably, the propylene glycol aqueous solution refers to a propylene glycol aqueous solution with the volume fraction of 40-60%.
Most preferably, the propylene glycol aqueous solution refers to a propylene glycol aqueous solution with a volume fraction of 50%.
Preferably, the dosage ratio of the dried black tiger fruits to the organic solvent in the step (1) is 1 g:10-20 mL.
Most preferably, the dosage ratio of the dried black tiger fruit to the organic solvent in the step (1) is 1g to 15mL.
Preferably, the extraction in the step (1) means that the heating reflux extraction is carried out at 95-100 ℃ for 2-3 hours.
Preferably, the dosage ratio of the organic solvent extract to water and ethyl acetate extraction and n-butanol in the step (2) is 1 g:30-50 mL:30-50 mL.
Most preferably, the ratio of organic solvent extract to water, ethyl acetate extract and n-butanol in step (2) is 1g:40mL:40 mL.
Preferably, further, the n-butanol extract is passed through a macroporous resin column; eluting with ethanol water solution with volume fraction of 6-8% and volume of 3-6 times of column volume to remove impurities; then eluting with ethanol water solution with the volume fraction of 16-18% and the volume fraction of 5-8 times of the column volume, collecting the eluent eluted from the ethanol water solution with the volume fraction of 16-18%, concentrating and drying to obtain the black tiger fruit extract with the whitening effect.
Most preferably, eluting with 5 times of column volume of 7% ethanol water solution to remove impurities; then eluting with 17% ethanol water solution with 6 times of column volume, collecting eluate eluted with 17% ethanol water solution, concentrating, and drying to obtain the final product.
The macroporous resin used in the invention is D101 macroporous resin.
In order to further improve the whitening effect of the black tiger fruit extract, the inventor further adopts macroporous resin to enrich active ingredients with the whitening effect on the basis of the n-butyl alcohol extract. Wherein the elution condition of macroporous resin plays a decisive role in preparing the black tiger fruit extract with more excellent whitening effect. The macroporous resin elution condition is not properly selected, so that the whitening effect of the prepared black tiger fruit extract cannot be further improved, but the whitening effect of the prepared black tiger fruit extract is smaller than that of the n-butanol extract.
Experiments by the inventor show that the black tiger fruit extract prepared only under the macroporous resin elution condition (namely, eluting the n-butanol extract by using an ethanol water solution with the volume fraction of 6-8 percent to remove impurities, and then eluting by using an ethanol water solution with the volume fraction of 16-18 percent) has excellent whitening effect, and the whitening effect is greatly improved compared with that of the n-butanol extract; the black tiger fruit extract prepared under the condition of eluting other macroporous resin does not have excellent whitening effect, and the whitening effect is not obviously improved or even greatly reduced compared with that of the n-butanol extract.
The invention also provides the black tiger fruit extract with the whitening effect, which is prepared by the preparation method.
The invention also provides an application of the black tiger fruit extract with the whitening effect in preparing the bath cream with the whitening effect.
The invention also provides an application of the black tiger fruit extract with the whitening effect in preparing tyrosinase inhibitors.
The beneficial effects are that: the invention provides a brand-new preparation method of a black tiger fruit extract, and the black tiger fruit extract prepared by the method has an excellent whitening effect. The black tiger fruit extract prepared by the invention has excellent whitening effect; therefore, the active ingredient of the active ingredient is added into the bath foam, so that the prepared bath foam has the whitening effect.
Detailed Description
The present invention is further explained below with reference to specific examples, which are not intended to limit the present invention in any way.
Example 1 preparation of Tiger fruit extract
(1) Taking dried black tiger fruits, adding 50% 1, 2-propylene glycol water solution, heating and reflux extracting for 2h at 100 ℃, concentrating and drying the extracting solution to obtain an organic solvent extract; wherein, the dosage ratio of the dried black tiger fruits to the 1, 2-propylene glycol aqueous solution is 1g to 15mL;
(2) Suspending the organic solvent extract with water; extracting with ethyl acetate, extracting with n-butanol, concentrating and drying n-butanol extract to obtain n-butanol extract, namely the black tiger fruit extract with whitening effect; wherein the dosage ratio of the organic solvent extract to water and ethyl acetate extraction and n-butanol is 1g:40mL:40 mL.
Example 2 preparation of Tiger fruit extract
(1) Taking dried black tiger fruits, adding 50% 1, 2-propylene glycol water solution, heating and reflux extracting for 2h at 100 ℃, concentrating and drying the extracting solution to obtain an organic solvent extract; wherein, the dosage ratio of the dried black tiger fruits to the 1, 2-propylene glycol aqueous solution is 1g to 15mL;
(2) Suspending the organic solvent extract with water; extracting with ethyl acetate, extracting with n-butanol, concentrating and drying n-butanol extract to obtain n-butanol extract, namely the black tiger fruit extract with whitening effect; wherein the dosage ratio of the organic solvent extract to water and ethyl acetate extraction to n-butanol is 1g:40mL:40 mL;
(3) Loading the n-butanol extract onto macroporous resin column (macroporous resin column is filled with macroporous resin of model D101, wherein the amount of macroporous resin is 50 times of the weight of the n-butanol extract), eluting with ethanol water solution with volume fraction of 7% and 5 times of column volume to remove impurities; then eluting with 17% ethanol water solution with 6 times of column volume, collecting eluate eluted with 17% ethanol water solution, concentrating, and drying to obtain the final product.
Example 3 preparation of Tiger fruit extract
(1) Taking dried black tiger fruits, adding a 1, 2-propylene glycol aqueous solution with the volume fraction of 40%, carrying out heating reflux extraction for 3 hours at 100 ℃, concentrating and drying the extract to obtain an organic solvent extract; wherein, the dosage ratio of the dried black tiger fruits to the 1, 2-propylene glycol aqueous solution is 1g to 20mL;
(2) Suspending the organic solvent extract with water; extracting with ethyl acetate, extracting with n-butanol, concentrating and drying n-butanol extract to obtain n-butanol extract, namely the black tiger fruit extract with whitening effect; wherein the dosage ratio of the organic solvent extract to water and ethyl acetate extraction to n-butanol is 1g:50mL:50 mL;
(3) Loading the n-butanol extract onto macroporous resin column (macroporous resin column is filled with macroporous resin of model D101, wherein the amount of macroporous resin is 50 times of the weight of the n-butanol extract), eluting with 3 times of ethanol aqueous solution with volume fraction of 6% to remove impurities; then eluting with 5 times of ethanol water solution with volume fraction of 18%, collecting eluate eluted with volume fraction of 18%, concentrating, and drying to obtain the extract with whitening effect.
Example 4 preparation of Tiger fruit extract
(1) Taking dried black tiger fruits, adding a 1, 2-propylene glycol aqueous solution with the volume fraction of 60%, heating and refluxing at 100 ℃ for 2.5 hours, concentrating and drying the extract to obtain an organic solvent extract; wherein, the dosage ratio of the dried black tiger fruits to the 1, 2-propylene glycol aqueous solution is 1g to 10mL;
(2) Suspending the organic solvent extract with water; extracting with ethyl acetate, extracting with n-butanol, concentrating and drying n-butanol extract to obtain n-butanol extract, namely the black tiger fruit extract with whitening effect; wherein the dosage ratio of the organic solvent extract to water to ethyl acetate extraction to n-butanol is 1g:30mL:30 mL;
(3) Loading the n-butanol extract onto macroporous resin column (macroporous resin column is filled with macroporous resin of model D101, wherein the amount of macroporous resin is 50 times of the weight of the n-butanol extract), eluting with 3 times of ethanol water solution with volume fraction of 8% to remove impurities; and eluting with ethanol water solution with volume fraction of 16% and volume fraction of 5 times of column volume, collecting eluate eluted with ethanol water solution with volume fraction of 16%, concentrating, and drying to obtain the extract.
Comparative example 1 preparation of black tiger fruit extract
(1) Taking dried black tiger fruits, adding an ethanol water solution with the volume fraction of 50%, carrying out heating reflux extraction for 2 hours at 78 ℃, concentrating and drying the extract to obtain an organic solvent extract; wherein, the dosage ratio of the dried black tiger fruits to the ethanol water solution is 1g to 15mL;
(2) Suspending the organic solvent extract with water; extracting with ethyl acetate, extracting with n-butanol, concentrating and drying n-butanol extract to obtain n-butanol extract, namely the black tiger fruit extract with whitening effect; wherein the dosage ratio of the organic solvent extract to water and ethyl acetate extraction and n-butanol is 1g:40mL:40 mL.
Comparative example 1 differs from example 1 in that comparative example 1 was first extracted with 50% aqueous ethanol and then extracted with n-butanol to obtain an n-butanol extract; in example 1, an aqueous solution of 50% 1, 2-propanediol was used for extraction, followed by extraction with n-butanol to obtain an n-butanol extract.
Comparative example 2 preparation of black tiger fruit extract
(1) Collecting dried black tiger fruit, adding water, heating and reflux extracting at 100deg.C for 2 hr, concentrating and drying to obtain extract; wherein the dosage ratio of the dried black tiger fruits to the water is 1g to 15mL;
(2) Suspending the extract in water; extracting with ethyl acetate, extracting with n-butanol, concentrating and drying n-butanol extract to obtain n-butanol extract, namely the black tiger fruit extract with whitening effect; wherein the dosage ratio of the extract to water and ethyl acetate extraction and n-butanol is 1g:40mL:40 mL.
Comparative example 2 differs from example 1 in that comparative example 2 was extracted with water followed by n-butanol to obtain an n-butanol extract; in example 1, an aqueous solution of 50% 1, 2-propanediol was used for extraction, followed by extraction with n-butanol to obtain an n-butanol extract.
Comparative example 3 preparation of extract of Tiger fruit
(1) Taking dried black tiger fruits, adding 1, 2-propylene glycol, heating and reflux-extracting for 2 hours at 100 ℃, concentrating and drying the extracting solution to obtain an organic solvent extract; wherein the dosage ratio of the dried black tiger fruits to the 1, 2-propylene glycol is 1g to 15mL;
(2) Suspending the organic solvent extract with water; extracting with ethyl acetate, extracting with n-butanol, concentrating and drying n-butanol extract to obtain n-butanol extract, namely the black tiger fruit extract with whitening effect; wherein the dosage ratio of the organic solvent extract to water and ethyl acetate extraction and n-butanol is 1g:40mL:40 mL.
Comparative example 3 differs from example 1 in that comparative example 3 was first extracted with 1, 2-propanediol and then extracted with n-butanol to obtain an n-butanol extract; in example 1, an aqueous solution of 50% 1, 2-propanediol was used for extraction, followed by extraction with n-butanol to obtain an n-butanol extract.
Comparative example 4 preparation of black tiger fruit extract
(1) Taking dried black tiger fruits, adding 50% 1, 2-propylene glycol water solution, heating and reflux extracting for 2h at 100 ℃, concentrating and drying the extracting solution to obtain an organic solvent extract; wherein, the dosage ratio of the dried black tiger fruits to the 1, 2-propylene glycol aqueous solution is 1g to 15mL;
(2) Suspending the organic solvent extract with water; extracting with ethyl acetate, extracting with n-butanol, concentrating and drying n-butanol extract to obtain n-butanol extract, namely the black tiger fruit extract with whitening effect; wherein the dosage ratio of the organic solvent extract to water and ethyl acetate extraction to n-butanol is 1g:40mL:40 mL;
(3) Loading the n-butanol extract onto macroporous resin column (macroporous resin column is filled with macroporous resin of model D101, wherein the amount of macroporous resin is 50 times of the weight of the n-butanol extract), eluting with 5 times of ethanol water solution with volume fraction of 5% to remove impurities; then eluting with ethanol water solution with volume fraction of 20% and volume fraction of 6 times of column volume, collecting eluate eluted with ethanol water solution with volume fraction of 20%, concentrating, and drying to obtain the extract with whitening effect.
Comparative example 4 differs from example 2 in that the elution conditions of the macroporous resin are different, and comparative example 4 first uses an aqueous ethanol solution with a volume fraction of 5% to elute the n-butanol extract to remove impurities; then eluting with 20% ethanol water solution; in the embodiment 2, the n-butanol extract is firstly eluted with 7% ethanol water solution by volume fraction to remove impurities; then eluting with 17% ethanol water solution.
Comparative example 5 preparation of black tiger fruit extract
(1) Taking dried black tiger fruits, adding 50% 1, 2-propylene glycol water solution, heating and reflux extracting for 2h at 100 ℃, concentrating and drying the extracting solution to obtain an organic solvent extract; wherein, the dosage ratio of the dried black tiger fruits to the 1, 2-propylene glycol aqueous solution is 1g to 15mL;
(2) Suspending the organic solvent extract with water; extracting with ethyl acetate, extracting with n-butanol, concentrating and drying n-butanol extract to obtain n-butanol extract, namely the black tiger fruit extract with whitening effect; wherein the dosage ratio of the organic solvent extract to water and ethyl acetate extraction to n-butanol is 1g:40mL:40 mL;
(3) Loading the n-butanol extract onto macroporous resin column (macroporous resin column is filled with macroporous resin of model D101, wherein the amount of macroporous resin is 50 times of the weight of the n-butanol extract), eluting with ethanol water solution with volume fraction of 15% and 5 times of column volume to remove impurities; then eluting with ethanol water solution with volume fraction of 25% and volume fraction of 6 times of column volume, collecting eluate eluted with ethanol water solution with volume fraction of 25%, concentrating, and drying to obtain the extract with whitening effect.
Comparative example 5 differs from example 2 in that the elution conditions of the macroporous resin are different, and the comparative example 5 first uses an aqueous ethanol solution with a volume fraction of 15% to elute the n-butanol extract to remove impurities; then eluting with 25% ethanol water solution; in the embodiment 2, the n-butanol extract is firstly eluted with 7% ethanol water solution by volume fraction to remove impurities; then eluting with 17% ethanol water solution.
Comparative example 6 preparation of black tiger fruit extract
(1) Taking dried black tiger fruits, adding 50% 1, 2-propylene glycol water solution, heating and reflux extracting for 2h at 100 ℃, concentrating and drying the extracting solution to obtain an organic solvent extract; wherein, the dosage ratio of the dried black tiger fruits to the 1, 2-propylene glycol aqueous solution is 1g to 15mL;
(2) Suspending the organic solvent extract with water; extracting with ethyl acetate, extracting with n-butanol, concentrating and drying n-butanol extract to obtain n-butanol extract, namely the black tiger fruit extract with whitening effect; wherein the dosage ratio of the organic solvent extract to water and ethyl acetate extraction to n-butanol is 1g:40mL:40 mL;
(3) Loading the n-butanol extract onto macroporous resin column (macroporous resin column is filled with macroporous resin of type D101, wherein the amount of macroporous resin is 50 times of the weight of the n-butanol extract), eluting with ethanol water solution with volume fraction of 30% and 5 times of column volume to remove impurities; then eluting with 60% ethanol water solution with 6 times of column volume, collecting eluate eluted with 60% ethanol water solution, concentrating, and drying to obtain the final product.
Comparative example 6 differs from example 2 in that the elution conditions of the macroporous resin are different, and comparative example 6 first elutes the n-butanol extract with an aqueous ethanol solution having a volume fraction of 30% to remove impurities; then eluting with 60% ethanol water solution; in the embodiment 2, the n-butanol extract is firstly eluted with 7% ethanol water solution by volume fraction to remove impurities; then eluting with 17% ethanol water solution.
Experimental example whitening experiment test of black tiger fruit extract
(1) Preparing an L-dopa solution with a preparation concentration of 1g/L by adopting 0.2mol/L phosphate buffer solution (pH of 6.8);
(2) Preparing tyrosinase solution with the concentration of 100U/mL;
(3) Preparing a liquid medicine to be detected with the concentration of 2 mg/mL;
(4) Adding 40 mu L of L-dopa solution, 40 mu L of liquid medicine to be detected and/or 40 mu L of tyrosinase solution into a 96-well plate, and then reacting for 40min in a water bath at 30 ℃; after the reaction is completed, the absorbance A is tested at 490nm, each group of experiments is repeated for 3 times, and the absorbance of each group of experiments is taken as the average value of 3 times of tests; calculating tyrosinase inhibition rate; wherein the liquid medicine to be detected is the black tiger fruit extract prepared by adopting examples 1-4 and comparative examples 1-6; the test results are shown in Table 1; the higher the tyrosinase inhibition rate is, the better the whitening effect of the black tiger fruit extract prepared by the reaction is.
Wherein, tyrosinase inhibition rate= [1- (A) T1 -A T2 )/(A C1 -A C2 )]x100%; in the formula, A T1 The absorbance is measured under the conditions of adding tyrosinase solution and adding liquid medicine to be measured; a is that T2 Absorbance measured without tyrosinase solution (replaced by equivalent phosphate buffer solution) and with the liquid medicine to be measured; a is that C1 Absorbance measured with tyrosinase solution but without the test solution (replaced with equivalent phosphate buffer solution); a is that C2 The absorbance was measured without adding tyrosinase solution and without adding the liquid medicine to be measured (replaced by the same amount of phosphate buffer solution).
TABLE 1 results of tyrosinase inhibitory Activity test of extract of Tiger fruit
| Liquid medicine to be measured | Tyrosinase inhibition rate |
| The extract of Tiger fruit prepared in example 1 | 68.48％ |
| The extract of Tiger fruit prepared in example 2 | 91.56％ |
| Example 3 preparation of Tiger fruit extract | 82.27％ |
| The extract of Tiger fruit prepared in example 4 | 88.14％ |
| Comparative example 1 extract of Tiger fruit | 19.48％ |
| Comparative example 2 extract of Tiger fruit | 12.16％ |
| Comparative example 3 extract of Tiger fruit | 35.72％ |
| Comparative example 4 extract of Tiger fruit | 65.39％ |
| Comparative example 5 extract of Tiger fruit | 44.65％ |
| Comparative example 6 extract of Tiger fruit | 18.12％ |
As can be seen from the experimental data in Table 1, the tyrosinase inhibition rate of the black tiger fruit extract prepared in example 1 is far higher than that of the black tiger fruit extracts prepared in comparative examples 1 to 3; this illustrates: the selection of the organic solvent plays a decisive role in preparing the black tiger fruit extract with higher tyrosinase inhibitory activity and excellent whitening effect. Only the n-butanol extract obtained by extracting dried black tiger fruits with propylene glycol aqueous solution and then n-butanol extraction has higher tyrosinase inhibition activity and excellent whitening effect; the black tiger fruit extract prepared by adopting common solvents such as ethanol, water and the like or adopting propylene glycol does not have higher tyrosinase inhibitory activity and excellent whitening effect.
As can be seen from the experimental data in Table 1, the tyrosinase inhibition rate of the black tiger fruit extracts prepared in examples 2 to 4 is more than 80%, and the tyrosinase inhibition rate is further improved greatly compared with that of example 1. The method is characterized in that the dry black tiger fruits are extracted by adopting the propylene glycol aqueous solution, then the dry black tiger fruits are extracted by n-butanol, and the tyrosinase inhibition rate of the black tiger fruits extract prepared under the macroporous resin elution condition can be further greatly improved, so that the prepared black tiger fruits extract has excellent whitening effect.
As can be seen from the experimental data in table 1, the tyrosinase inhibition rate of the black tiger fruit extract prepared in comparative example 4 is far less than that of example 2, and even less than that of example 1; the extracts of the black tiger fruits prepared in comparative examples 5 and 6 have a much smaller tyrosinase inhibition rate than that of example 2 and are much smaller than that of example 1, and do not have excellent tyrosinase inhibition activity. This illustrates: in the invention, the elution condition of macroporous resin plays a decisive role in preparing the black tiger fruit extract with more excellent tyrosine inhibition rate and whitening effect. The macroporous resin elution condition is not properly selected, so that the tyrosine inhibition rate and the whitening effect of the prepared black tiger fruit extract are not improved greatly, but the tyrosinase inhibition activity and the whitening effect of the prepared black tiger fruit extract are smaller than those of the n-butanol extract. Only under the macroporous resin elution condition (namely, eluting the n-butanol extract by using an ethanol water solution with the volume fraction of 6-8 percent to remove impurities, and then eluting by using an ethanol water solution with the volume fraction of 16-18 percent) the black tiger fruit extract prepared by the macroporous resin elution condition has very excellent tyrosinase inhibitory activity and whitening effect, but the black tiger fruit extract prepared under other macroporous resin elution conditions does not have very excellent tyrosinase inhibitory activity and whitening effect.
Claims (9)
1. A preparation method of a black tiger fruit extract with a whitening effect is characterized by comprising the following steps:
(1) Extracting dried black tiger fruit with organic solvent, concentrating and drying the organic solvent extract to obtain organic solvent extract; the organic solvent is propylene glycol aqueous solution; the propylene glycol aqueous solution refers to a propylene glycol aqueous solution with the volume fraction of 50%;
(2) Suspending the organic solvent extract with water; extracting with ethyl acetate, extracting with n-butanol, concentrating and drying n-butanol extract to obtain n-butanol extract;
further, the n-butanol extract is put on a macroporous resin column; eluting with ethanol aqueous solution with the volume fraction of 6-8% and the volume of 3-6 times of the column volume to remove impurities; and eluting with an ethanol water solution with the volume fraction of 16-18% and the volume fraction of 5-8 times of the column volume, collecting the eluent eluted from the ethanol water solution with the volume fraction of 16-18%, concentrating and drying to obtain the black tiger fruit extract with the whitening effect.
2. The method for preparing the black tiger fruit extract with the whitening effect according to claim 1, wherein the dosage ratio of the dry black tiger fruits to the organic solvent in the step (1) is 1 g:10-20 mL.
3. The method for preparing a black tiger fruit extract with whitening effect according to claim 2, wherein the dosage ratio of the dry black tiger fruit to the organic solvent in the step (1) is 1g to 15ml.
4. The method for preparing the black tiger fruit extract with the whitening effect according to claim 1, wherein the dosage ratio of the organic solvent extract to water, ethyl acetate extraction and n-butanol in the step (2) is 1 g:30-50 mL:30-50 mL.
5. The method of claim 4, wherein the ratio of the organic solvent extract to water, ethyl acetate extract and n-butanol in step (2) is 1g:40mL:40 mL.
6. The method for preparing a black tiger fruit extract with whitening effect according to claim 1, wherein eluting with 5 times of ethanol water solution with volume fraction of 7% to remove impurities; then eluting with 17% ethanol water solution with 6 times of column volume, collecting eluate eluted with 17% ethanol water solution, concentrating, and drying to obtain the final product.
7. The black tiger fruit extract with whitening effect prepared by the preparation method of any one of claims 1 to 6.
8. The use of the extract of black tiger fruit having whitening effect of claim 7 for preparing bath cream having whitening effect.
9. The use of the extract of black tiger fruit having whitening effect of claim 7 for preparing tyrosinase inhibitor.
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