CN110538247A - a method for extracting total flavone from pollen Typhae - Google Patents
a method for extracting total flavone from pollen Typhae Download PDFInfo
- Publication number
- CN110538247A CN110538247A CN201810527765.9A CN201810527765A CN110538247A CN 110538247 A CN110538247 A CN 110538247A CN 201810527765 A CN201810527765 A CN 201810527765A CN 110538247 A CN110538247 A CN 110538247A
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- cattail pollen
- extract
- ethanol
- total flavone
- pollen extract
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
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- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a preparation method for extracting total flavonoids from cattail pollen, which is characterized by comprising the following steps: 1) grinding dried pollen Typhae leaf into coarse powder; 2) taking the coarse powder, adding 100ml of absolute ethyl alcohol, performing ultrasonic treatment for 3 times with the power of 90-180 w, performing 2 hours each time, combining extracting solutions, and recovering the ethyl alcohol to obtain a cattail pollen extract; 3) extraction: adding a proper amount of distilled water into the cattail pollen extract, uniformly stirring, adding the cattail pollen extract into a chromatographic column, adjusting the flow rate to be 8ml/min, washing the cattail pollen extract with distilled water to remove impurities, and desorbing the cattail pollen extract by using 30-60% ethanol as a mobile phase; 4) recovering ethanol to obtain total flavone extract, and vacuum drying to obtain total flavone. The total flavone prepared by the method has high product purity, good quality, simple and easy process method, and is suitable for industrial production.
Description
Technical Field
the invention belongs to the technical field of medicines, and particularly relates to a preparation method of total flavonoids.
Background
Yellow is dry pollen of Typha orientalis L, Typha orientalis Presl or plants of the same genus, and has effects of removing blood stasis, stopping bleeding, and treating stranguria, and can be used for treating hematemesis, epistaxis, hemoptysis, metrorrhagia, traumatic hemorrhage, amenorrhea, abdominal pain, traumatic injury, and stranguria with pain. The main chemical components of the composition comprise isorhamnetin, quercetin, flavonoid glycoside and the like. The main active ingredients in the cattail pollen are alkaloids, flavonoids, sterols, polysaccharides and the like, wherein the flavonoid compound is one of the main functional components and has very important effect on the exertion of a plurality of pharmacological effects.
in recent years, an ultrasonic extraction method is widely applied to extraction of effective components in natural plants as an excellent extraction method, and compared with a Soxhlet extraction method, a reflux extraction method and an ultrasonic extraction method for extracting total flavonoids, the result shows that the ultrasonic extraction method is higher in extraction rate than the Soxhlet extraction method and the reflux extraction method, and is shorter in time, so that the ultrasonic extraction method is more effective and simpler in method for extracting the total flavonoids.
Disclosure of Invention
The invention aims to provide a method for extracting total flavonoids, and the total flavonoids have wide medicinal and health-care values.
the invention is completed by the following technical scheme, and the specific content comprises the following steps: method for extracting total flavone
1) Grinding dried pollen Typhae into coarse powder;
2) Taking the coarse powder, adding 100ml of absolute ethyl alcohol, performing ultrasonic treatment for 3 times with the power of 90-180 w, performing 2 hours each time, combining extracting solutions, and recovering the ethyl alcohol to obtain a cattail pollen extract;
3) Extraction: adding a proper amount of distilled water into the cattail pollen extract, uniformly stirring, adding the cattail pollen extract into a chromatographic column, adjusting the flow rate to be 8ml/min, washing the cattail pollen extract with distilled water to remove impurities, and desorbing the cattail pollen extract by using 30-60% ethanol as a mobile phase;
4) Recovering ethanol to obtain total flavone extract, and vacuum drying to obtain total flavone.
the power of the ultrasonic wave in the step 2) is 90-180W.
The chromatographic column in the step 3) is prepared by pretreating D101 type macroporous resin and polyamide, and the content of the mobile phase desorption ethanol is 30-60%.
The invention has the advantages that
The invention adopts ultrasonic extraction-D101 type macroporous resin and polyamide column chromatography to extract the total flavone in the cattail pollen, the sample capacity of the column chromatography is large, the extraction efficiency is high, and the method is simple and easy to implement.
The present invention will be further described with reference to specific embodiments, but the scope of the invention as claimed is not limited to the following embodiments.
the specific implementation mode is as follows:
Example 1:
grinding dried pollen Typhae into coarse powder; adding 100ml of absolute ethanol, performing ultrasonic treatment for 3 times at a power of 90w for 2h each time, mixing the extractive solutions, and recovering ethanol to obtain pollen Typhae extract; adding appropriate amount of distilled water into pollen Typhae extract, stirring, adding into chromatographic column, adjusting flow rate to 8ml/min, washing with distilled water to remove impurities, and desorbing with 30% ethanol as mobile phase; recovering ethanol to obtain total flavone extract, and vacuum drying to obtain total flavone with extraction rate of 95.3%.
Example 2:
Grinding dried pollen Typhae into coarse powder; adding 100ml of absolute ethanol, performing ultrasonic treatment for 3 times at a power of 180w for 2h each time, mixing the extractive solutions, and recovering ethanol to obtain pollen Typhae extract; adding appropriate amount of distilled water into pollen Typhae extract, stirring, adding into chromatographic column, adjusting flow rate to 8ml/min, washing with distilled water to remove impurities, and desorbing with 50% ethanol as mobile phase; recovering ethanol to obtain total flavone extract, and vacuum drying to obtain total flavone with extraction rate of 91.4%.
example 3:
Grinding dried pollen Typhae into coarse powder; adding 100ml of absolute ethanol, performing ultrasonic treatment for 3 times at a power of 120w for 2h each time, mixing the extractive solutions, and recovering ethanol to obtain pollen Typhae extract; adding appropriate amount of distilled water into pollen Typhae extract, stirring, adding into chromatographic column, adjusting flow rate to 8ml/min, washing with distilled water to remove impurities, and desorbing with 60% ethanol as mobile phase; recovering ethanol to obtain total flavone extract, and vacuum drying to obtain total flavone with extraction rate of 92.0%.
Claims (3)
1. A preparation method for extracting total flavonoids from cattail pollen is characterized by comprising the following steps:
1) Grinding dried pollen Typhae into coarse powder;
2) taking the coarse powder, adding 100ml of absolute ethyl alcohol, performing ultrasonic treatment for 3 times with the power of 90-180 w, performing 2 hours each time, combining extracting solutions, and recovering the ethyl alcohol to obtain a cattail pollen extract;
3) Extraction: adding a proper amount of distilled water into the cattail pollen extract, uniformly stirring, adding the cattail pollen extract into a chromatographic column, adjusting the flow rate to be 8ml/min, washing the cattail pollen extract with distilled water to remove impurities, and desorbing the cattail pollen extract by using 30-60% ethanol as a mobile phase;
4) Recovering ethanol to obtain total flavone extract, and vacuum drying to obtain total flavone.
2. The method for extracting total flavonoids from cattail pollen according to claim 1, wherein the ultrasonic power in step 2) is 90-180W.
3. The method for extracting total flavonoids from cattail pollen according to claim 1, wherein the chromatographic column in step 3) is prepared by pretreating D101 type macroporous resin and polyamide, and the content of mobile phase-desorbed ethanol is 30-60%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810527765.9A CN110538247A (en) | 2018-05-29 | 2018-05-29 | a method for extracting total flavone from pollen Typhae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810527765.9A CN110538247A (en) | 2018-05-29 | 2018-05-29 | a method for extracting total flavone from pollen Typhae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110538247A true CN110538247A (en) | 2019-12-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810527765.9A Pending CN110538247A (en) | 2018-05-29 | 2018-05-29 | a method for extracting total flavone from pollen Typhae |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116077570A (en) * | 2023-02-02 | 2023-05-09 | 河北瑞龙生物科技有限公司 | Preparation method of pollen typhae extract |
-
2018
- 2018-05-29 CN CN201810527765.9A patent/CN110538247A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116077570A (en) * | 2023-02-02 | 2023-05-09 | 河北瑞龙生物科技有限公司 | Preparation method of pollen typhae extract |
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| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20191206 |