CN114252321B - Dyeing method for medical experiment - Google Patents
Dyeing method for medical experiment Download PDFInfo
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- CN114252321B CN114252321B CN202111624391.0A CN202111624391A CN114252321B CN 114252321 B CN114252321 B CN 114252321B CN 202111624391 A CN202111624391 A CN 202111624391A CN 114252321 B CN114252321 B CN 114252321B
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- 238000004043 dyeing Methods 0.000 title claims abstract description 50
- 238000002474 experimental method Methods 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 12
- 230000007246 mechanism Effects 0.000 claims abstract description 68
- 238000011010 flushing procedure Methods 0.000 claims abstract description 45
- 239000007788 liquid Substances 0.000 claims abstract description 34
- 238000012544 monitoring process Methods 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 87
- 238000002347 injection Methods 0.000 claims description 20
- 239000007924 injection Substances 0.000 claims description 20
- 238000004140 cleaning Methods 0.000 claims description 13
- 239000002699 waste material Substances 0.000 claims description 9
- 238000001514 detection method Methods 0.000 claims description 7
- 238000010186 staining Methods 0.000 claims description 3
- 238000003825 pressing Methods 0.000 claims description 2
- 238000007447 staining method Methods 0.000 claims 3
- 239000000523 sample Substances 0.000 description 108
- 239000000243 solution Substances 0.000 description 11
- 238000005406 washing Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 239000012192 staining solution Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000006101 laboratory sample Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Cosmetics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a medical experiment dyeing method, which comprises the steps of 1, starting a first suction mechanism to fill a first dye solution into a sample area, and flushing the sample area after standing for a set time to finish dyeing of the first dye solution; 2, starting a second dye liquor suction pump to fill a second dye liquor into the sample area, and flushing the sample area after standing for a set time to finish dyeing of the second dye liquor; 3, starting a third dye liquor suction pump to fill a third dye liquor into the sample area, monitoring the color below the sample area in real time through a camera, and flushing the sample area when the color limit value is reached to the point number, so as to finish the dyeing of the third dye liquor; and 4, starting the second suction mechanism to fill the fourth dye liquor into the sample area, and flushing the sample area after standing for a set time to finish the sample adding of the fourth dye liquor, so that the sample can be used for microscopic examination. According to the invention, the manual operation is replaced by multi-station automatic filling of the dyeing liquid, so that the dyeing stability, consistency and color recognition accuracy are greatly improved, and the error of a dyeing result is avoided.
Description
Technical Field
The invention relates to dyeing of medical experiment samples, in particular to a medical experiment dyeing method.
Background
The dyeing of the medical experiment sample is mainly performed manually at present, and comprises the following four steps; step 1, coating a sample on a sample area of a sample container, airing, dripping a first dye solution, standing for a preset time, and washing with water; step 2, dripping a second dye solution into the sample area, standing for a preset time, and washing with water; step 3, tilting the sample container, slowly dripping the third dye solution from the upper part of the sample area to enable the third dye solution to flow through the sample area until the third dye solution flowing down from the sample area becomes colorless, and then washing with water; and step 4, dripping a fourth dye solution into the sample area, standing for a preset time, washing with water, airing in the air to finish dyeing, and sending to a microscope for detection.
The defects of the method are as follows:
1. the sample container is manually taken, the staining solution is dripped into the staining solution bottle, the sample container is manually held and washed in the cleaning solution tank, the operation is complicated, and the stability and the consistency are poor; and in direct contact with medical laboratory samples and chemical reagents, there is a risk of biohazard.
2. Dyeing of the sample is finished by atomizing the dyeing liquid through the spray head, but the dyeing liquid is easy to crystallize, so that the spray head is blocked, and the maintenance difficulty is high; in addition, the step 3 requires fixed time, and the third dye liquor filling amount cannot be adjusted according to the actual state of the sample, so that the step 3 is excessively or insufficiently dyed, and the risk of misdiagnosis of a patient is easily caused;
3. the third dye liquor is added dropwise, and the third dye liquor is fixed in time, so that the filling amount of the third dye liquor cannot be adjusted according to the actual state of the sample, excessive or insufficient dyeing is caused, the risk of misdiagnosis of a patient is caused, and the third dye liquor is wasted.
Disclosure of Invention
The invention aims to provide a multi-station medical experiment dyeing method with high automation degree.
In order to achieve the above purpose, the present invention may adopt the following technical scheme:
the medical experiment dyeing method adopts the following medical experiment dyeing system;
the medical experiment staining system comprises:
a first dye liquor filling pipe for filling a first dye liquor into the sample area; the first dye liquor filling pipeline comprises a first dye liquor accommodating bottle and a first dye liquor filling port; the first dye liquor accommodating bottle inner cavity is communicated with the first dye liquor filling port through a first suction mechanism and a first filling pipeline, and the first dye liquor filling port is communicated with the cleaning bucket through a three-way joint, a first dye liquor flushing pipeline and a first water suction pump; the first dye liquor flushing pipeline is used for flushing the first filling pipeline with water;
a second dye liquor filling pipe for filling a second dye liquor into the sample area; the second dye liquor filling pipeline comprises a second dye liquor accommodating bottle and a second dye liquor filling port; the inner cavity of the second dye liquor accommodating bottle is communicated with the second dye liquor filling port through a second dye liquor suction pump and a second filling pipeline;
a third dye liquor filling pipe for filling a third dye liquor into the sample area; the third dye liquor filling pipeline comprises a third dye liquor accommodating bottle and a third dye liquor filling port; the inner cavity of the third dye liquor accommodating bottle is communicated with the third dye liquor filling port through a third dye liquor suction pump and a third filling pipeline;
a fourth dye liquor filling pipe for filling a fourth dye liquor into the sample area; the fourth dye liquor filling pipeline comprises a fourth dye liquor accommodating bottle and a fourth dye liquor filling port; the inner cavity of the fourth dye liquor accommodating bottle is communicated with the fourth dye liquor filling port through a second suction mechanism and a fourth filling pipeline;
the water flushing pipeline is used for performing water injection cleaning on the sample area; the water flushing pipeline comprises the cleaning water bucket and a water injection port; the water injection port is communicated with the cleaning water bucket through a water injection pipeline and a second water suction pump;
the movable bracket is used for conveying the sample container to the positions below the first dye liquor filling port, the second dye liquor filling port, the third dye liquor filling port, the fourth dye liquor filling port and the water filling port according to the dyeing experiment requirements; the movable bracket is provided with a tilting mechanism which is used for enabling the sample container to be switched between a horizontal state and a tilting state;
a camera is arranged above the third dye liquor filling port and is used for monitoring the color change below the sample area in real time and sending the color change to an upper computer;
the medical experiment dyeing method comprises the following steps:
step 1, starting a first suction mechanism to inject a first dye liquor into a first filling pipeline, and suspending when the first filling pipeline is full of the first dye liquor; then operating the movable bracket to horizontally move the sample container to the position below the first filling opening, starting the first suction mechanism again, opening the first dye liquor filling opening to fill the first dye liquor into the sample area, and stopping the first suction mechanism after the first dye liquor covers the sample area; after standing for a set time, operating the movable bracket to move the sample container to the position below the water filling port, operating the tilting mechanism to enable the sample container to be in a tilting state, starting the second water suction pump, flushing the sample area through the water filling port, and operating the tilting mechanism to enable the sample container to be in a horizontal state after flushing is completed, so as to complete the dyeing of the first dye liquor; after dyeing is completed, a first water suction pump is started to flush a first dye liquor flushing pipeline;
step 2, starting a second dye liquor suction pump to inject a second dye liquor into the second filling pipeline, and stopping when the second filling pipeline is full of the second dye liquor; then operating the movable bracket to horizontally move the sample container to the position below the second filling port, starting the second dye liquor suction pump again, opening the second dye liquor filling port to fill the second dye liquor into the sample area, and stopping the second dye liquor suction pump after the second dye liquor covers the sample area; after standing for a set time, operating the movable bracket to move the sample container to the position below the water filling port, operating the tilting mechanism to enable the sample container to be in a tilting state, starting the second water suction pump, flushing the sample area through the water filling port, and operating the tilting mechanism to enable the sample container to be in a horizontal state after flushing is completed, so as to complete dyeing of the second dye liquor;
step 3, starting a third dye liquor suction pump to inject a third dye liquor into the third filling pipeline, and suspending when the third filling pipeline is full of the third dye liquor; then, operating the movable bracket to horizontally move the sample container to the position below the third filling port, operating the tilting mechanism to enable the sample container to be in a tilting state, starting the third dye liquor suction pump again, opening the third dye liquor filling port to fill the third dye liquor into the sample area, enabling the third dye liquor to flow down from the sample area, simultaneously monitoring the color below the sample area in real time through the camera, dividing a detection area into a plurality of color points by the upper computer according to a set color judgment algorithm, and stopping the third dye liquor suction pump when the detection area reaches the color point number of the color limit value or is larger than/equal to the set color point number; at the moment, a sample container on the movable bracket is positioned below the water filling port, a second water suction pump is started, a sample area is flushed through the water filling port, and after the flushing is finished, the sample container is in a horizontal state by operating the tilting mechanism, so that the third dye liquor dyeing is finished;
step 4, starting a second suction mechanism to inject a fourth dye liquor into the fourth filling pipeline, and suspending when the fourth filling pipeline is full of the fourth dye liquor; then operating the movable bracket to horizontally move the sample container to the position below the fourth filling opening, starting the fourth suction mechanism again, opening the fourth dye liquor filling opening to fill the fourth dye liquor into the sample area, and stopping the second suction mechanism after the fourth dye liquor covers the sample area; and after the sample container is placed for a set time, the movable bracket is operated to move the sample container to the lower part of the water injection port, the tilting mechanism is operated to enable the sample container to be in a tilting state, the second water suction pump is started, the sample area is flushed through the water injection port, after the flushing is finished, the tilting mechanism is operated to enable the sample container to be in a horizontal state, and after the sample area is dried in the air, the fourth dye liquor sample adding is finished, so that the sample container can be used for microscopic examination.
Further, the first suction mechanism and the second suction mechanism have the same structure and comprise a cam or other actuating mechanisms driven by a stepping motor to rotate and a push-type liquid suction check valve, wherein the cam or other actuating mechanisms are used for pressing a push-type liquid suction check valve button, and a liquid outlet of the push-type liquid suction check valve is communicated with the first dye liquor filling pipeline or the fourth dye liquor filling pipeline.
Further, the first dye liquor filling port, the second dye liquor filling port, the third dye liquor filling port, the fourth dye liquor filling port and the water filling port are arranged above the waste liquid pool, and the waste liquid pool is communicated with the waste liquid barrel through a liquid discharge pipe.
Further, the mobile carriage comprises a wheeled carriage arranged on a track, a sample rack arranged on the wheeled carriage for carrying the sample container; the tilting mechanism is a screw rod or an electric push rod which is upwards arranged and driven by a motor, and the upper end of the screw rod or the electric push rod is contacted with the middle area of the sample frame.
The invention has the advantages that the dyeing liquid is automatically filled to replace manual operation, liberate manpower and improve the dyeing stability and consistency; the first dye liquor flushing pipeline is adopted to flush the first filling pipeline, so that the problem of crystallization blockage in the pipeline and the filling port after the first dye liquor is filled is solved. By adopting a scheme of color real-time monitoring feedback, the accuracy of the dyeing result is improved, doctors are helped to accurately judge the sample dyeing result, misdiagnosis is reduced, and patients are benefited. The suction pump and the stepping motor are adopted to drive the push-type liquid-pumping check valve, so that quantitative and micro accurate filling of the dyeing liquid is realized, and the dyeing liquid is saved more. In the step 3, the sample container is inclined at a certain angle during dyeing and flushing, so that the third dye liquor and flushing water can flow through the sample area, the color recognition accuracy is greatly improved, and the error of the dyeing result is avoided.
Drawings
FIG. 1 is a schematic structural diagram of a medical laboratory staining system according to the present invention.
Fig. 2 is a schematic diagram of an assembled structure of the mobile carriage and tilting mechanism of the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that all directional indicators (such as up, down, left, right, front, and rear … …) in the embodiments of the present invention are merely used to explain the relative positional relationship, movement, etc. between the components in a particular posture (as shown in the drawings), and if the particular posture is changed, the directional indicator is changed accordingly.
In the present invention, unless specifically stated and limited otherwise, the terms "connected," "affixed," and the like are to be construed broadly, and for example, "affixed" may be a fixed connection, a removable connection, or an integral body; can be mechanically or electrically connected; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
Furthermore, descriptions such as those referred to as "first," "second," and the like, are provided for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implying an order of magnitude of the indicated technical features in the present disclosure. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature.
In addition, the meaning of "and/or" as it appears throughout is meant to include three side-by-side schemes, for example, "a and/or B", including a scheme, or B scheme, or a scheme that is satisfied by both a and B. In addition, the technical solutions of the embodiments may be combined with each other, but it is necessary to base that the technical solutions can be realized by those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent and not within the scope of protection claimed in the present invention.
As shown in fig. 1-2, the medical experiment dyeing method adopts the following medical experiment dyeing system;
a first dye liquor filling pipe 1 for filling a sample area with a first dye liquor; the first dye liquor filling pipeline 1 comprises a first dye liquor accommodating bottle 1.1 and a first dye liquor filling port 1.2; the inner cavity of the first dye liquor accommodating bottle 1.1 is communicated with a first dye liquor filling port 1.2 through a first suction mechanism and a first filling pipeline 1, and the first dye liquor filling port 1.2 is communicated with a cleaning bucket 1.6 through a three-way joint 1.3, a first dye liquor flushing pipeline 1.4 and a first water suction pump 1.5; a first dye liquor flushing pipe 1.4 for flushing the first filling pipe 1 with water;
the first suction mechanism consists of a cam 1.8 driven by a first stepping motor 1.7 to rotate and a push-type liquid suction check valve 1.9, wherein the cam 1.8 is used for driving a button of the push-type liquid suction check valve 1.9 to move up and down, and a liquid outlet of the push-type liquid suction check valve 1.9 is communicated with the first dye liquor filling pipeline 1; the first stepper motor 1.7 is selected to accurately control the flow, pressure and flow rate of the first dye liquor filling port 1.2 through subdivision control of the stepper motor;
a second dye liquor filling pipe 2 for filling a second dye liquor into the sample area; the second dye liquor filling pipeline 2 comprises a second dye liquor accommodating bottle 2.1 and a second dye liquor filling port 2.2; the inner cavity of the second dye liquor accommodating bottle 2.1 is communicated with a second dye liquor filling port 2.2 through a second dye liquor suction pump 2.3 and a second filling pipeline 2;
a third dye liquor filling pipe 3 for filling a third dye liquor into the sample area; the third dye liquor filling pipeline 3 comprises a third dye liquor accommodating bottle 3.1 and a third dye liquor filling port 3.2; the inner cavity of the third dye liquor accommodating bottle 3.1 is communicated with a third dye liquor filling port 3.2 through a third dye liquor suction pump 3.3 and a third filling pipeline 3; in order to increase the filling area of the third dye liquor, the third dye liquor suction pump 3.3 is selected as a double-channel pump (a single-channel pump can be used for replacing the pump without limiting the type of the pump), and the third dye liquor filling port 3.2 is formed by two parallel connection;
a fourth dye liquor filling pipe 4 for filling a fourth dye liquor into the sample area; the fourth dye liquor filling pipeline 4 comprises a fourth dye liquor accommodating bottle 4.1 and a fourth dye liquor filling port 4.2; the inner cavity of the fourth dye liquor accommodating bottle 4.1 is communicated with a fourth dye liquor filling port 4.2 through a second suction mechanism and a fourth filling pipeline 4;
the second suction mechanism consists of a cam 4.4 driven by a second stepping motor 4.3 to rotate and a push-type liquid suction check valve 4.5, wherein the cam 4.4 is used for driving a push-type liquid suction check valve 4.5 button to move up and down, and a liquid outlet of the push-type liquid suction check valve 4.5 is communicated with a fourth dye liquor filling pipeline 4; the second stepper motor 4.3 is selected to precisely control the flow, pressure and flow rate of the fourth dye liquor filling port 4.2 through control subdivision of the stepper motor;
a water flushing pipe 5 for water injection cleaning of the sample area; the water flushing pipeline 5 comprises a cleaning water bucket 1.6 and a water injection port 5.1; the water filling port 5.1 is communicated with the cleaning water bucket 1.6 through a flushing pipeline and a second water suction pump 5.2; in order to increase the flushing area, the second water pump 5.2 is selected as a double-channel pump (a single-channel pump can be used instead, and the type of the pump is not limited), and the water injection port 5.1 is formed by two water injection ports connected in parallel.
The first dye liquor filling port 1.2, the second dye liquor filling port 2.2, the third dye liquor filling port 3.2, the fourth dye liquor filling port 4.2 and the water filling port 5.1 are arranged above the waste liquid pool 6, and the waste liquid pool 6 is communicated with the waste liquid barrel 6.2 through the liquid discharge pipe 6.1.
As shown in fig. 2, the movable bracket is used for conveying the sample container 7 to the lower parts of the first dye liquor filling port 1.2, the second dye liquor filling port 2.2, the third dye liquor filling port 3.2, the fourth dye liquor filling port 4.2 and the water filling port 5.1 according to the dyeing experiment requirement.
The mobile carriage comprises a wheeled carriage 9 arranged on a track 8, on which wheeled carriage 9 a sample rack 9.1 for carrying the sample containers 7 and a tilting mechanism are arranged; the tilting mechanism is used to switch the sample container 7 between a horizontal state and a tilted state.
The tilting mechanism is a screw rod (of course, an electric push rod or other telescopic mechanisms) which is arranged upwards and driven by a motor 9.2, and a roller at the upper end of the screw rod is contacted with the middle area of the sample frame 9.1.
A camera is arranged above the third dye liquor filling port 3.2 and used for monitoring the color change of the third dye liquor in the sample area in real time and sending the color change to an upper computer.
The medical experiment dyeing method comprises the following steps:
step 1, starting a first suction mechanism to inject a first dye liquor into a first filling pipeline 1, and suspending when the first filling pipeline 1 is full of the first dye liquor; then the movable bracket is operated to horizontally move the sample container 7 to the position below the first filling port 1.2, the first suction mechanism is started again, the first dye liquor filling port 1.2 is opened to fill the sample area of the sample container 7 with the first dye liquor, the first stepping motor 1.7 drives the cam 1.8 to rotate for one circle to drive the push button of the push-type liquor drawing check valve 1.9 to move up and down once, and filling is completed; after the sample container 7 is kept stand for a set time, the movable bracket is operated to move the sample container 7 to the position below the water injection port 5.1, the tilting mechanism is operated to enable the sample container 7 to be in a tilting state, the second water suction pump 5.2 is started, the sample area is flushed through the water injection port 5.1, and after the flushing is completed, the tilting mechanism is operated to enable the sample container to be in a horizontal state, so that the first dye liquor dyeing is completed; after a certain period of experiments are completed, before the equipment is closed, a first water suction pump 1.5 is started to flush the first dye liquor flushing pipeline 1;
step 2, starting a second dye liquor suction pump 2.3 to inject a second dye liquor into the second filling pipeline 2, and stopping when the second filling pipeline 2 is full of the second dye liquor; then operating the movable bracket to horizontally move the sample container 7 below the second filling port 2.2, starting the second dye liquor suction pump 2.3 again, opening the second dye liquor filling port 2.2 to fill the second dye liquor into the sample area, and stopping the second dye liquor suction pump 2.3 after the second dye liquor covers the sample area; after the sample container 7 is kept stand for a set time, the movable bracket is operated to move the sample container 7 to the position below the water injection port 5.1, the tilting mechanism is operated to enable the sample container 7 to be in a tilting state, the second water suction pump 5.2 is started, the sample area is flushed through the water injection port 5.1, and after the flushing is finished, the tilting mechanism is operated to enable the sample container 7 to be in a horizontal state, so that the second dye liquor dyeing is finished;
step 3, starting a third dye liquor suction pump 3.3 to inject a third dye liquor into the third filling pipeline 3, and stopping when the third filling pipeline 3 is full of the third dye liquor; then, the movable bracket is operated to horizontally move the sample container 7 to the position below the third filling port 3.2, the tilting mechanism is operated to enable the sample container 7 to be in a tilting state, the third dye liquor suction pump 3.3 is started again, the third dye liquor filling port 3.2 is opened to fill the third dye liquor into the sample area, the third dye liquor flows down from the sample area, meanwhile, the color below the sample area of the glass slide is monitored in real time through the camera, the upper computer divides the detection area into a plurality of color points according to a set color judgment algorithm, and when the detection area reaches the color point number of the color limit value or is larger than/equal to the set color point number, the third dye liquor suction pump 3.3 is stopped; because the third dye liquor filling port 3.2 and the water flushing pipeline water filling port 5.1 are both arranged in the area above the sample container 7, the sample container 7 on the movable bracket is positioned below the water filling port 5.1, the second water suction pump 5.2 is started, the sample area is flushed through the water filling port 5.1, and after the flushing is finished, the tilting mechanism is operated to enable the sample container 7 to be in a horizontal state, so that the third dye liquor dyeing is finished;
step 4, starting a second suction mechanism to inject a fourth dye liquor into the fourth filling pipeline 4, and suspending when the fourth filling pipeline 4 is full of the fourth dye liquor; then the movable bracket is operated to horizontally move the sample container 7 to the position below the fourth filling port 4.2, the fourth suction mechanism is started again, the fourth dye liquor filling port 4.2 is opened to fill the fourth dye liquor into the sample area, the second stepping motor 4.3 drives the cam 4.4 to rotate for one circle to drive the push-type liquor drawing check valve 4.5 button to move up and down once, and filling is completed; and after the sample container 7 is kept stand for a set time, the movable bracket is operated to move the sample container 7 to the position below the water injection port 5.1, the tilting mechanism is operated to enable the sample container 7 to be in a tilting state, the second water suction pump 5.2 is started, the sample area is flushed through the water injection port 5.1, after the flushing is finished, the tilting mechanism is operated to enable the sample container 7 to be in a horizontal state, and after the sample area is dried in the air, the fourth dye liquor sample adding is finished, so that the sample can be used for microscopic examination.
Claims (4)
1. A medical experiment dyeing method is characterized in that the following medical experiment dyeing system is adopted,
the medical experiment staining system comprises:
a first dye liquor filling pipe for filling a first dye liquor into the sample area; the first dye liquor filling pipeline comprises a first dye liquor accommodating bottle and a first dye liquor filling port; the first dye liquor accommodating bottle inner cavity is communicated with the first dye liquor filling port through a first suction mechanism and a first filling pipeline, and the first dye liquor filling port is communicated with the cleaning bucket through a three-way joint, a first dye liquor flushing pipeline and a first water suction pump; the first dye liquor flushing pipeline is used for flushing the first filling pipeline with water;
a second dye liquor filling pipe for filling a second dye liquor into the sample area; the second dye liquor filling pipeline comprises a second dye liquor accommodating bottle and a second dye liquor filling port; the inner cavity of the second dye liquor accommodating bottle is communicated with the second dye liquor filling port through a second dye liquor suction pump and a second filling pipeline;
a third dye liquor filling pipe for filling a third dye liquor into the sample area; the third dye liquor filling pipeline comprises a third dye liquor accommodating bottle and a third dye liquor filling port; the inner cavity of the third dye liquor accommodating bottle is communicated with the third dye liquor filling port through a third dye liquor suction pump and a third filling pipeline;
a fourth dye liquor filling pipe for filling a fourth dye liquor into the sample area; the fourth dye liquor filling pipeline comprises a fourth dye liquor accommodating bottle and a fourth dye liquor filling port; the inner cavity of the fourth dye liquor accommodating bottle is communicated with the fourth dye liquor filling port through a second suction mechanism and a fourth filling pipeline;
the water flushing pipeline is used for performing water injection cleaning on the sample area; the water flushing pipeline comprises the cleaning water bucket and a water injection port; the water injection port is communicated with the cleaning water bucket through a water injection pipeline and a second water suction pump;
the movable bracket is used for conveying the sample container to the positions below the first dye liquor filling port, the second dye liquor filling port, the third dye liquor filling port, the fourth dye liquor filling port and the water filling port according to the dyeing experiment requirements; the movable bracket is provided with a tilting mechanism which is used for enabling the sample container to be switched between a horizontal state and a tilting state;
a camera is arranged above the third dye liquor filling port and is used for monitoring the color change below the sample area in real time and sending the color change to an upper computer;
the medical experiment dyeing method comprises the following steps:
step 1, starting a first suction mechanism to inject a first dye liquor into a first filling pipeline, and suspending when the first filling pipeline is full of the first dye liquor; then operating the movable bracket to horizontally move the sample container to the position below the first filling opening, starting the first suction mechanism again, opening the first dye liquor filling opening to fill the first dye liquor into the sample area, and stopping the first suction mechanism after the first dye liquor covers the sample area; after standing for a set time, operating the movable bracket to move the sample container to the position below the water filling port, operating the tilting mechanism to enable the sample container to be in a tilting state, starting the second water suction pump, flushing a sample area through the water filling port, and operating the tilting mechanism to enable the sample container to be in a horizontal state after flushing is completed, so as to complete dyeing of the first dye liquor; after dyeing is completed, a first water suction pump is started to flush a first dye liquor flushing pipeline;
step 2, starting a second dye liquor suction pump to inject a second dye liquor into the second filling pipeline, and stopping when the second filling pipeline is full of the second dye liquor; then operating the movable bracket to horizontally move the sample container to the position below the second filling port, starting the second dye liquor suction pump again, opening the second dye liquor filling port to fill the second dye liquor into the sample area, and stopping the second dye liquor suction pump after the second dye liquor covers the sample area; after standing for a set time, operating the movable bracket to move the sample container to the position below the water filling port, operating the tilting mechanism to enable the sample container to be in a tilting state, starting the second water suction pump, flushing the sample area through the water filling port, and operating the tilting mechanism to enable the sample container to be in a horizontal state after flushing is completed, so as to complete dyeing of the second dye liquor;
step 3, starting a third dye liquor suction pump to inject a third dye liquor into the third filling pipeline, and suspending when the third filling pipeline is full of the third dye liquor; then, operating the movable bracket to horizontally move the sample container to the position below the third filling port, operating the tilting mechanism to enable the sample container to be in a tilting state, starting the third dye liquor suction pump again, opening the third dye liquor filling port to fill the third dye liquor into the sample area, enabling the third dye liquor to flow down from the sample area, simultaneously monitoring the color below the sample area in real time through the camera, dividing a detection area into a plurality of color points by the upper computer according to a set color judgment algorithm, and stopping the third dye liquor suction pump when the detection area reaches the color point number of the color limit value or is larger than/equal to the set color point number; at the moment, a sample container on the movable bracket is positioned below the water filling port, a second water suction pump is started, a sample area is flushed through the water filling port, and after the flushing is finished, the sample container is in a horizontal state by operating the tilting mechanism, so that the third dye liquor dyeing is finished;
step 4, starting a second suction mechanism to inject a fourth dye liquor into the fourth filling pipeline, and suspending when the fourth filling pipeline is full of the fourth dye liquor; then operating the movable bracket to horizontally move the sample container to the position below the fourth filling opening, starting the fourth suction mechanism again, opening the fourth dye liquor filling opening to fill the fourth dye liquor into the sample area, and stopping the second suction mechanism after the fourth dye liquor covers the sample area; and after standing for a set time, operating the movable bracket to move the sample container to the lower part of the water filling port, operating the tilting mechanism to enable the sample container to be in a tilting state, starting the second water suction pump, flushing the sample area through the water filling port, operating the tilting mechanism to enable the sample container to be in a horizontal state after flushing is completed, and finishing fourth dye liquor dyeing after airing the sample area, so that the sample container can be used for microscopic examination.
2. The medical laboratory staining method of claim 1, wherein: the first suction mechanism and the second suction mechanism have the same structure and comprise a cam or other actuating mechanisms and a push-type liquid suction check valve, wherein the cam or other actuating mechanisms are driven by a stepping motor to rotate, the cam or other actuating mechanisms are used for pressing a push-type liquid suction check valve button, and a liquid outlet of the push-type liquid suction check valve is communicated with a first dye liquor filling pipeline or a fourth dye liquor filling pipeline.
3. The medical laboratory staining method of claim 1 or 2, wherein: the first dye liquor filling port, the second dye liquor filling port, the third dye liquor filling port, the fourth dye liquor filling port and the water filling port are arranged above the waste liquid pool, and the waste liquid pool is communicated with the waste liquid barrel through a liquid discharge pipe.
4. The medical laboratory staining method of claim 1 or 2, wherein: the movable bracket comprises a wheel bracket arranged on a track, and a sample rack arranged on the wheel bracket and used for bearing the sample container; the tilting mechanism is a screw rod or an electric push rod which is upwards arranged and driven by a motor, and the upper end of the screw rod or the electric push rod is contacted with the middle area of the sample frame.
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| JPH0928395A (en) * | 1995-07-19 | 1997-02-04 | Toshiba Corp | Dyeing of organism and apparatus therefor |
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