CN114235803A - Decolorization determination method used after microbial staining - Google Patents

Decolorization determination method used after microbial staining Download PDF

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Publication number
CN114235803A
CN114235803A CN202111523352.1A CN202111523352A CN114235803A CN 114235803 A CN114235803 A CN 114235803A CN 202111523352 A CN202111523352 A CN 202111523352A CN 114235803 A CN114235803 A CN 114235803A
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decoloring
liquid
decolorization
sample
slide
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CN114235803B (en
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王聪
唐鹏桢
袁梦阳
王超
刘聪
魏文娟
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Autobio Labtec Instruments Zhengzhou Co Ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84Systems specially adapted for particular applications
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • G01N2021/0106General arrangement of respective parts
    • G01N2021/0112Apparatus in one mechanical, optical or electronic block

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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Abstract

The invention discloses a decolorization judgment method used after microbial staining, which comprises the steps of continuously flushing an obliquely placed sample slide by adopting a one-way flowing decolorization solution to decolorize the sample, collecting RGB (red, green and blue) three-color data of each pixel point of a detected area of the sample slide in real time in the decolorization process, converting the RGB three-color data into a gray value, comparing the gray value with a set gray threshold value, and determining a decolorization endpoint at a moment when 65-85% of gray values are more than the gray threshold value; and if the decoloring is still reached after the predetermined time, taking the predetermined time as the decoloring end point. The invention carries out scouring and decoloring on the surface of the sample by the decoloring liquid flowing in one direction, and simultaneously carries out real-time monitoring on the color change condition of the sample by the camera module, and judges the decoloring end point in time, thereby greatly improving the decoloring quality and the decoloring effect. The invention has strong adaptability to different samples, high decolorization success rate, strong detection precision and stability and greatly improves the accuracy of microorganism detection.

Description

Decolorization determination method used after microbial staining
Technical Field
The invention relates to the technical field of microbial detection, in particular to a decolorization determination method used after microbial staining.
Background
The bacteria are tiny, colorless and transparent, so the size and morphological structure of the bacteria are usually observed by a staining method. Gram stain and acid fast stain are two commonly used bacterial staining methods, both of which include the steps of staining cells and then destaining, wherein the quality of staining and destaining has a large effect on the test results. Most of the existing laboratories adopt manual dyeing, and because the abilities and experiences of operators are different, the problems of different dyeing efficiency and dyeing effect still occur according to the same operation rules. The decoloring operation comprises two methods of timing decoloring and photodiode detection of a decoloring endpoint, wherein the fixed-dosage decoloring solution and the same decoloring time are adopted in the decoloring operation, the adaptability to different dyed samples is poor, the decoloring effect is poor, and more decoloring solution needs to be consumed; the latter needs to collect the diluted destaining solution, and the detection has certain hysteresis, and the accuracy and precision of destaining judgment are not enough.
Disclosure of Invention
In order to solve the above problems, the present invention provides a method for accurately determining a decolorization end point after microbial staining, which specifically adopts the following technical scheme:
the invention relates to a decolorization judgment method used after microbial staining, which is characterized in that a sample slide obliquely placed is continuously washed by a one-way flowing decolorization liquid for decolorization treatment of a sample, RGB (red, green and blue) three-color data of each pixel point of a detected area of the sample slide are collected in real time and converted into gray values in the decolorization process, the gray values are compared with a set gray threshold value, and when the gray values exceeding 65-85% at a certain moment are all larger than the gray threshold value, the moment is a decolorization terminal point; and if the decoloring is still reached to the decoloring end point after the predetermined time, taking the predetermined time as the decoloring end point.
The decolorization determination method is realized by a decolorization determination device which comprises a supporting platform provided with a decolorization determination device
The sample placing part is provided with a liquid inlet end and a liquid discharging end which is positioned obliquely below the liquid inlet end, and a slide clamping groove is arranged between the liquid inlet end and the liquid discharging end;
a liquid injection part which is provided with a liquid injection port arranged close to the liquid inlet end;
a detection section having a camera module disposed above the slide card slot.
A white bottom plate is arranged in the glass slide clamping groove, and supporting columns with the height of 2-4mm are arranged at four corners of the white bottom plate respectively.
And a liquid discharge hole is formed in the liquid discharge end of the sample placing part.
The sample placing part is connected with the supporting platform through a first adjusting and supporting component, and the first adjusting and supporting component comprises
The hinged seat is provided with a horizontal hinged shaft, one end of the hinged seat is connected with the supporting platform, and the other end of the hinged seat is connected with the liquid discharge end of the sample placing part;
the supporting seat comprises a connecting seat connected with the supporting platform, wherein a vertical adjusting screw is arranged on the connecting seat, the vertical adjusting screw is close to the liquid inlet end of the sample placing part, and the top of the vertical adjusting screw is connected with the bottom surface of the sample placing part.
Annotate the liquid mouth and include that the play liquid direction is vertical decurrent first notes liquid mouth and second and annotate the liquid mouth, first notes liquid mouth links to each other with pure water supply portion, the second is annotated the liquid mouth and is linked to each other with decoloration liquid supply portion.
Annotate liquid portion and link to each other with supporting platform through second regulation supporting component, second regulation supporting component includes
The first supporting frame is vertically arranged and is connected with the supporting platform in a sliding way,
the second support frame is of an L-shaped structure, one end of the second support frame is connected with the liquid injection part, and the other end of the second support frame is connected with the first support frame in a sliding manner;
the first support frame moves close to or far away from the sample placing part along the support platform, and the second support frame vertically moves along the first support frame.
The camera module is provided with a color CMOS image sensor with 30 ten thousand pixels, and a light supplement lamp is arranged around the camera module.
The detection part is arranged on the door-shaped frame, the free end of the door-shaped frame is provided with an adjusting long hole, and the width of the adjusting long hole is matched with the outer diameter of the connecting shaft arranged on the side face of the sample placing part.
The connecting shafts are arranged on the side surface of the sample placing part at intervals along the liquid flow direction.
According to the method for determining the decolorization after microbial dyeing, provided by the invention, the surface of the sample is washed and decolorized by the one-way flowing decolorization liquid, and meanwhile, the color change condition of the sample is monitored in real time by the camera module, so that the decolorization end point is determined in time, and the decolorization quality and the decolorization effect are greatly improved. The invention has strong adaptability to different samples, high decolorization success rate, strong detection precision and stability and greatly improves the accuracy of microorganism detection.
Drawings
FIG. 1 is a first schematic view of the structure of a decoloring determining apparatus according to the present invention.
FIG. 2 is a second schematic view showing the structure of the decoloring determining apparatus according to the present invention.
Detailed Description
The invention relates to a decolorization judgment method used after microbial staining, which is characterized in that a sample slide obliquely placed is continuously washed by a one-way flowing decolorization liquid for decolorization treatment of a sample, RGB (red, green and blue) three-color data of each pixel point in a detected area of the sample slide are collected in real time (usually collected after 2-5 seconds of decolorization) and converted into gray values in the decolorization process, the gray values are compared with a set gray threshold value, and when the gray values exceeding 65-85% at a certain moment are all larger than the gray threshold value, the moment is a decolorization end point; if the decoloring is still reached beyond a predetermined time (usually 8 to 15 seconds), the predetermined time is taken as the decoloring end point.
The invention also comprises a decoloring judgment device applying the decoloring judgment method, wherein the decoloring judgment device comprises a supporting platform, and a sample placing part, a liquid injection part and a detection part are arranged on the supporting platform.
The following describes embodiments of the present invention in detail with reference to the drawings, which are implemented on the premise of the technical solution of the present invention, and give detailed implementation manners and specific working procedures, but the scope of the present invention is not limited to the following embodiments.
Example 1:
as shown in fig. 1 and 2, the supporting platform has adjustable supporting legs, the sample placing part 1 thereon is a strip-shaped block-shaped member for placing a slide, the strip-shaped block-shaped member is obliquely arranged above the supporting platform through a first adjusting supporting component, the top part is a liquid inlet end, the bottom part is a liquid outlet end provided with a liquid outlet hole, and a slide clamping groove 101 is arranged at the middle position. In order to highlight the sample and enhance the contrast between the background and the sample, a white bottom plate is arranged in the slide clamping groove 101, and a material which is not easy to react with an organic solvent is adopted, so that the color of the slide clamping groove is not easy to change, and the stable performance is kept. The four corners of the white bottom plate are respectively provided with a supporting column 102 with the height of 2-4mm, and the glass slide is arranged on the supporting columns, so that the condition that the judgment is influenced by obvious watermarks between the glass slide and the bottom plate can be avoided. The first adjusting and supporting assembly comprises a hinged seat 201 and a supporting seat 202, wherein the hinged seat 201 is provided with a horizontal hinged shaft, one end of the hinged seat is connected with the supporting platform, and the other end of the hinged seat is connected with the liquid discharging end of the sample placing part 1; the supporting seat 202 comprises a connecting seat connected with the supporting platform, a vertical adjusting screw rod is arranged on the connecting seat, the vertical adjusting screw rod is close to the liquid inlet end of the sample placing part 1, and the top of the vertical adjusting screw rod is connected with the bottom surface of the sample placing part 1. Through rotating articulated seat 201, can adjust the inclination of portion 1 is place to the sample, afterwards, twist the adjusting screw of supporting seat 202 to suitable height, carry out the rigidity to portion 1 is place to the sample.
The liquid injection part 3 is provided with a liquid injection port close to the liquid inlet end, and comprises a first liquid injection port connected with the purified water supply part and a second liquid injection port connected with the destaining liquid supply part, and the liquid injection ports all discharge liquid downwards along the vertical direction. The liquid injection part 3 is connected with the supporting platform through a second adjusting and supporting assembly, the second adjusting and supporting assembly comprises a first supporting frame 401 which is vertically arranged and is connected with the supporting platform in a sliding manner; the second support frame 402 is of an L-shaped structure, one end of the second support frame is connected with the liquid injection part 3, and the other end of the second support frame is connected with the first support frame 401 in a sliding manner; the first support frame 401 moves along the support platform to approach or separate from the sample placement portion 1, and the second support frame 402 moves vertically along the first support frame 401.
The detection section includes a single chip microcomputer that performs data processing, and a camera module 501 having a color CMOS image sensor of 30 ten thousand pixels, which is located above the slide card slot 101; in order to provide sufficient illumination for the sample area, 6-12 LED lamps are also installed around the camera module 501 as fill-in lamps 502. The detection part is arranged at the top of the door-shaped frame 6, and the door-shaped frame 6 is connected with the sample placing part 1. Specifically, two free ends of the door-shaped frame 6 are respectively provided with an adjusting long hole 601, a plurality of connecting shafts 602 (the connecting shafts 602 are distributed along the liquid flow direction) are arranged on the side surface of the sample placing part 1 at intervals, the width of the adjusting long holes 601 is matched with the outer diameter of the connecting shafts 602, and the relative position of the detection part and the slide can be adjusted by selecting different connecting shafts 602 and changing the relative position of the adjusting long holes 601 and the connecting shafts 602.
Example 2:
when the device works, firstly, a slide is placed in the slide clamping groove 101, the inclination angle of the sample placing part 1 is adjusted through the first adjusting and supporting assembly (the included angle between the sample placing part 1 and the horizontal plane is generally 20-50 degrees, preferably 27 degrees), and the relative position between the liquid injection part 3 and the slide is adjusted through the first adjusting and supporting assembly (the vertical distance between the liquid injection port and the slide is generally 1-5cm, preferably 1.8 cm), so that purified water or destaining solution can be uniformly spread on the slide with the width of 2.5 cm; secondly, fully wetting the surface of the slide by using purified water, and then continuously flushing the surface of the slide by using a decoloring solution to decolor the sample; thirdly, after 2 seconds of decolorization, detecting the color change of each pixel point in the detected area of the slide in real time through a detection part, and giving out decolorization completion judgment through data processing; specifically, RGB three-color data of each pixel point in a detected area of a sample slide are collected in real time and converted into a gray value, the gray value is compared with a set gray threshold, and when more than 76% of gray values at a certain moment are all larger than the gray threshold, the moment is a decoloration endpoint; if the decoloration is still up to the decoloration end point after exceeding 15 seconds, taking 15 seconds as the decoloration end point; in the process, the real-time detection is started after 2-5 seconds of decolorization is selected, because the color of the sample is changed from colorless to bluish purple and then colorless in the decolorization process, the corresponding gray value is changed from large to small and then large, if the decolorization is started, the synchronous detection is carried out, the gray values of most pixel points are larger than the gray threshold value, and the misjudgment is easy to occur; and fourthly, after the decoloring is finished, closing the detection part, washing the slide clean by using purified water, and then taking out the slide from the slide clamping groove 101 to finish the detection operation.
Example 2:
when the device works, firstly, a slide is placed in the slide clamping groove 101, the inclination angle of the sample placing part 1 is adjusted through the first adjusting and supporting assembly, and the relative position of the liquid injection part 3 and the slide is adjusted through the first adjusting and supporting assembly, so that purified water or destaining solution can be uniformly spread on the slide with the width of 2.5 cm; secondly, fully wetting the surface of the slide by using purified water, and then continuously flushing the surface of the slide by using a decoloring solution to decolor the sample; thirdly, after 5 seconds of decolorization, detecting the color change of each pixel point in the detected area of the slide in real time through a detection part, and giving out decolorization completion judgment through data processing; specifically, RGB three-color data of each pixel point in a detected area of a sample slide are collected in real time and converted into a gray value, the gray value is compared with a set gray threshold, and when more than 85% of gray values at a certain moment are all larger than the gray threshold, the moment is a decoloration endpoint; if the decoloration is still up to the decoloration end point after exceeding 15 seconds, taking 15 seconds as the decoloration end point; in the process, the real-time detection is started after 2-5 seconds of decolorization is selected, because the color of the sample is changed from colorless to bluish purple and then colorless in the decolorization process, the corresponding gray value is changed from large to small and then large, if the decolorization is started, the synchronous detection is carried out, the gray values of most pixel points are larger than the gray threshold value, and the misjudgment is easy to occur; and fourthly, after the decoloring is finished, closing the detection part, washing the slide clean by using purified water, and then taking out the slide from the slide clamping groove 101 to finish the detection operation.
Example 3:
when the device works, firstly, a slide is placed in the slide clamping groove 101, the inclination angle of the sample placing part 1 is adjusted through the first adjusting and supporting assembly, and the relative position of the liquid injection part 3 and the slide is adjusted through the first adjusting and supporting assembly, so that purified water or destaining solution can be uniformly spread on the slide with the width of 2.5 cm; secondly, fully wetting the surface of the slide by using purified water, and then continuously flushing the surface of the slide by using a decoloring solution to decolor the sample; thirdly, after 2 seconds of decolorization, detecting the color change of each pixel point in the detected area of the slide in real time through a detection part, and giving out decolorization completion judgment through data processing; specifically, RGB three-color data of each pixel point in a detected area of a sample slide are collected in real time and converted into a gray value, the gray value is compared with a set gray threshold, and when more than 65% of gray values at a certain moment are all larger than the gray threshold, the moment is a decoloration endpoint; if the decoloration is still up to the decoloration end point after exceeding 8 seconds, taking 8 seconds as the decoloration end point; in the process, the real-time detection is started after 2-5 seconds of decolorization is selected, because the color of the sample is changed from colorless to bluish purple and then colorless in the decolorization process, the corresponding gray value is changed from large to small and then large, if the decolorization is started, the synchronous detection is carried out, the gray values of most pixel points are larger than the gray threshold value, and the misjudgment is easy to occur; and fourthly, after the decoloring is finished, closing the detection part, washing the slide clean by using purified water, and then taking out the slide from the slide clamping groove 101 to finish the detection operation.
Example 4:
when the device works, firstly, a slide is placed in the slide clamping groove 101, the inclination angle of the sample placing part 1 is adjusted through the first adjusting and supporting assembly, and the relative position of the liquid injection part 3 and the slide is adjusted through the first adjusting and supporting assembly, so that purified water or destaining solution can be uniformly spread on the slide with the width of 2.5 cm; secondly, fully wetting the surface of the slide by using purified water, and then continuously flushing the surface of the slide by using a decoloring solution to decolor the sample; thirdly, after decoloring for 3 seconds, detecting the color change of each pixel point in the detected area of the glass slide in real time through a detection part, and processing data to judge the completion of decoloring; specifically, RGB three-color data of each pixel point in a detected area of a sample slide are collected in real time and converted into a gray value, the gray value is compared with a set gray threshold, and when more than 70% of gray values at a certain moment are all larger than the gray threshold, the moment is a decoloration end point; if the decoloration is still up to the decoloration end point after exceeding 10 seconds, taking 10 seconds as the decoloration end point; in the process, the real-time detection is started after 2-5 seconds of decolorization is selected, because the color of the sample is changed from colorless to bluish purple and then colorless in the decolorization process, the corresponding gray value is changed from large to small and then large, if the decolorization is started, the synchronous detection is carried out, the gray values of most pixel points are larger than the gray threshold value, and the misjudgment is easy to occur; and fourthly, after the decoloring is finished, closing the detection part, washing the slide clean by using purified water, and then taking out the slide from the slide clamping groove 101 to finish the detection operation.
Example 5:
when the device works, firstly, a slide is placed in the slide clamping groove 101, the inclination angle of the sample placing part 1 is adjusted through the first adjusting and supporting assembly, and the relative position of the liquid injection part 3 and the slide is adjusted through the first adjusting and supporting assembly, so that purified water or destaining solution can be uniformly spread on the slide with the width of 2.5 cm; secondly, fully wetting the surface of the slide by using purified water, and then continuously flushing the surface of the slide by using a decoloring solution to decolor the sample; thirdly, after 5 seconds of decolorization, detecting the color change of each pixel point in the detected area of the slide in real time through a detection part, and giving out decolorization completion judgment through data processing; specifically, RGB three-color data of each pixel point in a detected area of a sample slide are collected in real time and converted into a gray value, the gray value is compared with a set gray threshold, and when more than 80% of gray values at a certain moment are all larger than the gray threshold, the moment is a decoloration end point; if the decoloration is still up to the decoloration end point after exceeding 15 seconds, taking 15 seconds as the decoloration end point; in the process, the real-time detection is started after 2-5 seconds of decolorization is selected, because the color of the sample is changed from colorless to bluish purple and then colorless in the decolorization process, the corresponding gray value is changed from large to small and then large, if the decolorization is started, the synchronous detection is carried out, the gray values of most pixel points are larger than the gray threshold value, and the misjudgment is easy to occur; and fourthly, after the decoloring is finished, closing the detection part, washing the slide clean by using purified water, and then taking out the slide from the slide clamping groove 101 to finish the detection operation.
It should be noted that in the description of the present invention, terms of orientation or positional relationship such as "front", "rear", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

Claims (10)

1. A method for determining decolorization after microbial staining, characterized in that: continuously flushing an obliquely placed sample slide by using a one-way flowing decoloring solution to decolor the sample, wherein in the decoloring process, RGB (red, green and blue) three-color data of each pixel point in a detected area of the sample slide are collected in real time and converted into gray values, the gray values are compared with a set gray threshold value, and when the gray values exceeding 65-85% of a certain moment are all larger than the gray threshold value, the moment is a decoloring end point; and if the decoloring is still reached to the decoloring end point after the predetermined time, taking the predetermined time as the decoloring end point.
2. The method for determining discoloration after microbial staining according to claim 1, wherein: the decolorization determination method is realized by a decolorization determination device which comprises a supporting platform provided with a decolorization determination device
The sample placing part is provided with a liquid inlet end and a liquid discharging end which is positioned obliquely below the liquid inlet end, and a slide clamping groove is arranged between the liquid inlet end and the liquid discharging end;
a liquid injection part which is provided with a liquid injection port arranged close to the liquid inlet end;
a detection section having a camera module disposed above the slide card slot.
3. The method for determining discoloration after microbial staining according to claim 2, wherein: a white bottom plate is arranged in the glass slide clamping groove, and supporting columns with the height of 2-4mm are arranged at four corners of the white bottom plate respectively.
4. The method for determining discoloration after microbial staining according to claim 2, wherein: and a liquid discharge hole is formed in the liquid discharge end of the sample placing part.
5. The method for determining discoloration after microbial staining according to claim 2, wherein: the sample placing part is connected with the supporting platform through a first adjusting and supporting component, and the first adjusting and supporting component comprises
The hinged seat is provided with a horizontal hinged shaft, one end of the hinged seat is connected with the supporting platform, and the other end of the hinged seat is connected with the liquid discharge end of the sample placing part;
the supporting seat comprises a connecting seat connected with the supporting platform, wherein a vertical adjusting screw is arranged on the connecting seat, the vertical adjusting screw is close to the liquid inlet end of the sample placing part, and the top of the vertical adjusting screw is connected with the bottom surface of the sample placing part.
6. The method for determining discoloration after microbial staining according to claim 2, wherein: annotate the liquid mouth and include that the play liquid direction is vertical decurrent first notes liquid mouth and second and annotate the liquid mouth, first notes liquid mouth links to each other with pure water supply portion, the second is annotated the liquid mouth and is linked to each other with decoloration liquid supply portion.
7. The method for determining discoloration after microbial staining according to claim 2, wherein: annotate liquid portion and link to each other with supporting platform through second regulation supporting component, second regulation supporting component includes
The first supporting frame is vertically arranged and is connected with the supporting platform in a sliding way,
the second support frame is of an L-shaped structure, one end of the second support frame is connected with the liquid injection part, and the other end of the second support frame is connected with the first support frame in a sliding manner;
the first support frame moves close to or far away from the sample placing part along the support platform, and the second support frame vertically moves along the first support frame.
8. The method for determining discoloration after microbial staining according to claim 2, wherein: the camera module is provided with a color CMOS image sensor with 30 ten thousand pixels, and a light supplement lamp is arranged around the camera module.
9. The method for determining discoloration after microbial staining according to claim 2, wherein: the detection part is arranged on the door-shaped frame, the free end of the door-shaped frame is provided with an adjusting long hole, and the width of the adjusting long hole is matched with the outer diameter of the connecting shaft arranged on the side face of the sample placing part.
10. The method for determining discoloration after microbial staining according to claim 9, wherein: the connecting shafts are arranged on the side surface of the sample placing part at intervals along the liquid flow direction.
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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10300675A (en) * 1997-04-30 1998-11-13 Agency Of Ind Science & Technol Method for evaluating degree of decolorization of waste fluid
US20070059844A1 (en) * 2005-09-12 2007-03-15 Techmer Pm, Llc Non-metameric color matching system and method
DE102012201265A1 (en) * 2012-01-30 2013-08-01 Henkel Ag & Co. Kgaa Reductive color print
CN109736111A (en) * 2018-12-29 2019-05-10 台山市红岭洗染有限公司 Denim fabric decoloration control method
CN109781747A (en) * 2019-03-05 2019-05-21 齐鲁工业大学 Cytologic specimen print dyeing effect detection method and system based on machine vision
CN111575112A (en) * 2020-06-05 2020-08-25 浙江旭璟健康科技有限公司 Chinese torreya oil decoloration device
CN112102421A (en) * 2020-09-07 2020-12-18 巫协森 Automatic tracking operation method and system for wastewater decolorization reaction
CN112414910A (en) * 2020-09-25 2021-02-26 黑龙江诺潽生物科技有限公司 Method for quantitatively analyzing and accurately controlling usage amount of decolorized activated carbon
CN112891013A (en) * 2021-01-27 2021-06-04 中国人民解放军空军军医大学 Device for injecting medicine into colorectal cavity of small rodent experimental animal

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10300675A (en) * 1997-04-30 1998-11-13 Agency Of Ind Science & Technol Method for evaluating degree of decolorization of waste fluid
US20070059844A1 (en) * 2005-09-12 2007-03-15 Techmer Pm, Llc Non-metameric color matching system and method
DE102012201265A1 (en) * 2012-01-30 2013-08-01 Henkel Ag & Co. Kgaa Reductive color print
CN109736111A (en) * 2018-12-29 2019-05-10 台山市红岭洗染有限公司 Denim fabric decoloration control method
CN109781747A (en) * 2019-03-05 2019-05-21 齐鲁工业大学 Cytologic specimen print dyeing effect detection method and system based on machine vision
CN111575112A (en) * 2020-06-05 2020-08-25 浙江旭璟健康科技有限公司 Chinese torreya oil decoloration device
CN112102421A (en) * 2020-09-07 2020-12-18 巫协森 Automatic tracking operation method and system for wastewater decolorization reaction
CN112414910A (en) * 2020-09-25 2021-02-26 黑龙江诺潽生物科技有限公司 Method for quantitatively analyzing and accurately controlling usage amount of decolorized activated carbon
CN112891013A (en) * 2021-01-27 2021-06-04 中国人民解放军空军军医大学 Device for injecting medicine into colorectal cavity of small rodent experimental animal

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