CN216525092U - A decoloration decision maker for behind microorganism dyeing - Google Patents
A decoloration decision maker for behind microorganism dyeing Download PDFInfo
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- CN216525092U CN216525092U CN202123129224.7U CN202123129224U CN216525092U CN 216525092 U CN216525092 U CN 216525092U CN 202123129224 U CN202123129224 U CN 202123129224U CN 216525092 U CN216525092 U CN 216525092U
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Abstract
The utility model discloses a decolorization judging device used after microbial staining, which comprises a supporting platform, wherein the supporting platform is provided with: the sample placing part is provided with a liquid inlet end and a liquid outlet end, and a slide clamping groove is arranged between the liquid inlet end and the liquid outlet end; a liquid injection part which is provided with a liquid injection port arranged close to the liquid inlet end; a detection section having a camera module disposed above the slide card slot. The utility model has simple structure and easy operation, the decoloration liquid can uniformly and stably flow through the surface of the sample to decolor the sample, and simultaneously, the camera module is used for monitoring the color change condition of the sample in real time and judging the decoloration end point in time, thereby greatly improving the decoloration quality and the decoloration effect. The utility model has strong adaptability to different samples, high decolorization success rate, strong detection precision and stability and greatly improves the accuracy of microorganism detection.
Description
Technical Field
The utility model relates to the technical field of microbial detection, in particular to a decolorization judging device used after microbial staining.
Background
The bacteria are tiny, colorless and transparent, so the size and morphological structure of the bacteria are usually observed by a staining method. Gram stain and acid fast stain are two commonly used bacterial staining methods, both of which include the steps of staining cells and then destaining, wherein the quality of staining and destaining has a large effect on the test results. Most of the existing laboratories adopt manual dyeing, and because the abilities and experiences of operators are different, the problems of different dyeing efficiency and dyeing effect still occur according to the same operation rules. The decoloring operation comprises two methods of timing decoloring and photodiode detection of a decoloring endpoint, wherein the fixed-dosage decoloring solution and the same decoloring time are adopted in the decoloring operation, the adaptability to different dyed samples is poor, the decoloring effect is poor, and more decoloring solution needs to be consumed; the latter needs to collect the diluted destaining solution, and the detection has certain hysteresis, and the accuracy and precision of destaining judgment are not enough.
Disclosure of Invention
In order to solve the above problems, the present invention provides a decolorization determination device for use after microbial staining, which has a good decolorization effect and accurate determination of a decolorization end point, and specifically adopts the following technical scheme:
the device for determining the decolorization after the microbial staining comprises a supporting platform, wherein the supporting platform is provided with a color filter
The sample placing part is provided with a liquid inlet end and a liquid outlet end, and a slide clamping groove is arranged between the liquid inlet end and the liquid outlet end;
a liquid injection part which is provided with a liquid injection port arranged close to the liquid inlet end;
a detection section having a camera module disposed above the slide card slot.
A white bottom plate is arranged in the glass slide clamping groove, and supporting columns with the height of 2-4mm are arranged at four corners of the white bottom plate respectively.
And a liquid discharge hole is formed in the liquid discharge end of the sample placing part.
The sample placing part is connected with the supporting platform through a first adjusting and supporting component, and the first adjusting and supporting component comprises
The hinged seat is provided with a horizontal hinged shaft, one end of the hinged seat is connected with the supporting platform, and the other end of the hinged seat is connected with the liquid discharge end of the sample placing part;
the supporting seat comprises a connecting seat connected with the supporting platform, wherein a vertical adjusting screw is arranged on the connecting seat, the vertical adjusting screw is close to the liquid inlet end of the sample placing part, and the top of the vertical adjusting screw is connected with the bottom surface of the sample placing part.
Annotate the liquid mouth and include that the play liquid direction is vertical decurrent first notes liquid mouth and second and annotate the liquid mouth, first notes liquid mouth links to each other with pure water supply portion, the second is annotated the liquid mouth and is linked to each other with decoloration liquid supply portion.
Annotate liquid portion and link to each other with supporting platform through second regulation supporting component, second regulation supporting component includes
The first supporting frame is vertically arranged and is connected with the supporting platform in a sliding way,
the second support frame is of an L-shaped structure, one end of the second support frame is connected with the liquid injection part, and the other end of the second support frame is connected with the first support frame in a sliding manner;
the first support frame moves close to or far away from the sample placing part along the support platform, and the second support frame vertically moves along the first support frame.
The camera module is provided with a color CMOS image sensor with 30 ten thousand pixels, and a light supplement lamp is arranged around the camera module.
The detection part is arranged on the door-shaped frame, the free end of the door-shaped frame is provided with an adjusting long hole, and the width of the adjusting long hole is matched with the outer diameter of the connecting shaft arranged on the side face of the sample placing part.
The connecting shafts are arranged on the side surface of the sample placing part at intervals along the liquid flow direction.
The decolorization judging device for the dyed microorganisms, provided by the utility model, has the advantages that the structure is simple, the operation is easy, the decolorization liquid can uniformly and stably flow through the surface of the sample to decolorize the sample, meanwhile, the change condition of the color of the sample is monitored in real time through the camera module, the decolorization endpoint is judged in time, and the decolorization quality and the decolorization effect are greatly improved. The utility model has strong adaptability to different samples, high decolorization success rate, strong detection precision and stability and greatly improves the accuracy of microorganism detection.
Drawings
Fig. 1 is a first schematic structural diagram of the present invention.
FIG. 2 is a second schematic structural diagram of the present invention.
Detailed Description
The following describes embodiments of the present invention in detail with reference to the accompanying drawings, which are implemented on the premise of the technical solution of the present invention, and give detailed implementation manners and specific working procedures, but the scope of the present invention is not limited to the following embodiments.
As shown in fig. 1 and 2, the decolorization determination device for microorganism staining according to the present invention includes a support platform with adjustable support legs, on which a sample placement portion, a liquid injection portion, and a detection portion are disposed.
Specifically, the sample placing part 1 is a strip-shaped block-shaped member for placing a glass slide, the sample placing part is obliquely arranged above the supporting platform through a first adjusting and supporting assembly, the top of the sample placing part is a liquid inlet end, the bottom of the sample placing part is a liquid outlet end provided with a liquid outlet hole, and a glass slide clamping groove 101 is formed in the middle of the sample placing part. In order to highlight the sample and enhance the contrast between the background and the sample, a white bottom plate is arranged in the slide clamping groove 101, and a material which is not easy to react with an organic solvent is adopted, so that the color of the slide clamping groove is not easy to change, and the stable performance is kept. The four corners of the white bottom plate are respectively provided with a supporting column 102 with the height of 2-4mm, and the glass slide is arranged on the supporting columns, so that the condition that the judgment is influenced by obvious watermarks between the glass slide and the bottom plate can be avoided. The first adjusting and supporting assembly comprises a hinged seat 201 and a supporting seat 202, wherein the hinged seat 201 is provided with a horizontal hinged shaft, one end of the hinged seat is connected with the supporting platform, and the other end of the hinged seat is connected with the liquid discharging end of the sample placing part 1; the supporting seat 202 comprises a connecting seat connected with the supporting platform, a vertical adjusting screw rod is arranged on the connecting seat, the vertical adjusting screw rod is close to the liquid inlet end of the sample placing part 1, and the top of the vertical adjusting screw rod is connected with the bottom surface of the sample placing part 1. Through rotating articulated seat 201, can adjust the inclination of portion 1 is place to the sample, afterwards, twist the adjusting screw of supporting seat 202 to suitable height, carry out the rigidity to portion 1 is place to the sample.
The liquid injection part 3 is provided with a liquid injection port close to the liquid inlet end, and comprises a first liquid injection port connected with the purified water supply part and a second liquid injection port connected with the destaining liquid supply part, and the liquid injection ports all discharge liquid downwards along the vertical direction. The liquid injection part 3 is connected with the supporting platform through a second adjusting and supporting assembly, the second adjusting and supporting assembly comprises a first supporting frame 401 which is vertically arranged and is connected with the supporting platform in a sliding manner; the second support frame 402 is an L-shaped structure, one end of which is connected with the liquid injection part 3, and the other end is connected with the first support frame 401 in a sliding manner; the first support frame 401 moves along the support platform to approach or separate from the sample placement portion 1, and the second support frame 402 moves vertically along the first support frame 401.
The detection section includes a single chip microcomputer that performs data processing, and a camera module 501 having a color CMOS image sensor of 30 ten thousand pixels, which is located above the slide card slot 101; in order to provide sufficient illumination for the sample area, 6-12 LED lamps are also installed around the camera module 501 as fill-in lamps 502. The detection part is arranged at the top of the door-shaped frame 6, and the door-shaped frame 6 is connected with the sample placing part 1. Specifically, two free ends of the door-shaped frame 6 are respectively provided with an adjusting long hole 601, a plurality of connecting shafts 602 (the connecting shafts 602 are distributed along the liquid flow direction) are arranged on the side surface of the sample placing part 1 at intervals, the width of the adjusting long holes 601 is matched with the outer diameter of the connecting shafts 602, and the relative position of the detection part and the slide can be adjusted by selecting different connecting shafts 602 and changing the relative position of the adjusting long holes 601 and the connecting shafts 602.
When the device works, firstly, a slide is placed in the slide clamping groove 101, the inclination angle of the sample placing part 1 is adjusted through the first adjusting and supporting assembly (the included angle between the sample placing part 1 and the horizontal plane is generally 20-50 degrees, preferably 27 degrees), and the relative position between the liquid injection part 3 and the slide is adjusted through the first adjusting and supporting assembly (the vertical distance between the liquid injection port and the slide is generally 1-5cm, preferably 1.8 cm), so that purified water or destaining solution can be uniformly spread on the slide with the width of 2.5 cm; secondly, fully wetting the surface of the slide by using purified water, and then continuously flushing the surface of the slide by using a decoloring solution to decolor the sample; thirdly, after 2-5 seconds of decolorization, detecting the color change of each pixel point in the detected area of the glass slide in real time through a detection part, and giving a decolorization completion judgment through data processing, wherein a sample which does not complete decolorization for more than 15 seconds takes 15 seconds as a decolorization endpoint; and fourthly, after the decoloring is finished, closing the detection part, washing the slide clean by using purified water, and then taking out the slide from the slide clamping groove 101 to finish the detection operation.
It should be noted that in the description of the present invention, terms of orientation or positional relationship such as "front", "rear", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
Claims (9)
1. The utility model provides a decoloration decision maker for after microbial staining which includes supporting platform, its characterized in that: the supporting platform is provided with
The sample placing part is provided with a liquid inlet end and a liquid outlet end, and a slide clamping groove is arranged between the liquid inlet end and the liquid outlet end;
a liquid injection part which is provided with a liquid injection port arranged close to the liquid inlet end;
a detection section having a camera module disposed above the slide card slot.
2. The apparatus for determining discoloration after microbial staining according to claim 1, wherein: a white bottom plate is arranged in the glass slide clamping groove, and supporting columns with the height of 2-4mm are arranged at four corners of the white bottom plate respectively.
3. The apparatus for determining discoloration after microbial staining according to claim 1, wherein: and a liquid discharge hole is formed in the liquid discharge end of the sample placing part.
4. The apparatus for determining discoloration after microbial staining according to claim 1, wherein: the sample placing part is connected with the supporting platform through a first adjusting and supporting component, and the first adjusting and supporting component comprises
The hinged seat is provided with a horizontal hinged shaft, one end of the hinged seat is connected with the supporting platform, and the other end of the hinged seat is connected with the liquid discharge end of the sample placing part;
the supporting seat comprises a connecting seat connected with the supporting platform, wherein a vertical adjusting screw is arranged on the connecting seat, the vertical adjusting screw is close to the liquid inlet end of the sample placing part, and the top of the vertical adjusting screw is connected with the bottom surface of the sample placing part.
5. The apparatus for determining discoloration after microbial staining according to claim 1, wherein: annotate the liquid mouth and include that the play liquid direction is vertical decurrent first notes liquid mouth and second and annotate the liquid mouth, first notes liquid mouth links to each other with pure water supply portion, the second is annotated the liquid mouth and is linked to each other with decoloration liquid supply portion.
6. The apparatus for determining discoloration after microbial staining according to claim 1, wherein: annotate liquid portion and link to each other with supporting platform through second regulation supporting component, second regulation supporting component includes
The first supporting frame is vertically arranged and is connected with the supporting platform in a sliding way,
the second support frame is of an L-shaped structure, one end of the second support frame is connected with the liquid injection part, and the other end of the second support frame is connected with the first support frame in a sliding manner;
the first support frame moves close to or far away from the sample placing part along the support platform, and the second support frame vertically moves along the first support frame.
7. The apparatus for determining discoloration after microbial staining according to claim 1, wherein: the camera module is provided with a color CMOS image sensor with 30 ten thousand pixels, and a light supplement lamp is arranged around the camera module.
8. The apparatus for determining discoloration after microbial staining according to claim 1, wherein: the detection part is arranged on the door-shaped frame, the free end of the door-shaped frame is provided with an adjusting long hole, and the width of the adjusting long hole is matched with the outer diameter of the connecting shaft arranged on the side face of the sample placing part.
9. The apparatus for determining discoloration after microbial staining according to claim 8, wherein: the connecting shafts are arranged on the side surface of the sample placing part at intervals along the liquid flow direction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123129224.7U CN216525092U (en) | 2021-12-14 | 2021-12-14 | A decoloration decision maker for behind microorganism dyeing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123129224.7U CN216525092U (en) | 2021-12-14 | 2021-12-14 | A decoloration decision maker for behind microorganism dyeing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216525092U true CN216525092U (en) | 2022-05-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202123129224.7U Active CN216525092U (en) | 2021-12-14 | 2021-12-14 | A decoloration decision maker for behind microorganism dyeing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216525092U (en) |
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2021
- 2021-12-14 CN CN202123129224.7U patent/CN216525092U/en active Active
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