CN114231406A - Bacteria-algae coupling integrated equipment and oil production and decontamination method - Google Patents

Bacteria-algae coupling integrated equipment and oil production and decontamination method Download PDF

Info

Publication number
CN114231406A
CN114231406A CN202111561896.7A CN202111561896A CN114231406A CN 114231406 A CN114231406 A CN 114231406A CN 202111561896 A CN202111561896 A CN 202111561896A CN 114231406 A CN114231406 A CN 114231406A
Authority
CN
China
Prior art keywords
bacteria
algae
reactor
suspension
water
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202111561896.7A
Other languages
Chinese (zh)
Other versions
CN114231406B (en
Inventor
李立欣
付然
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangsu Tongyong Environment Engineering Co ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN202111561896.7A priority Critical patent/CN114231406B/en
Publication of CN114231406A publication Critical patent/CN114231406A/en
Application granted granted Critical
Publication of CN114231406B publication Critical patent/CN114231406B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G, C10K; LIQUEFIED PETROLEUM GAS; ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE OR UNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL; FIRELIGHTERS
    • C10L1/00—Liquid carbonaceous fuels
    • C10L1/02—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M43/00—Combinations of bioreactors or fermenters with other apparatus
    • C12M43/08—Bioreactors or fermenters combined with devices or plants for production of electricity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/001—Upstream control, i.e. monitoring for predictive control
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/005—Processes using a programmable logic controller [PLC]
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/02—Temperature
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/06—Controlling or monitoring parameters in water treatment pH
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/16—Total nitrogen (tkN-N)
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/18—PO4-P
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/38—Gas flow rate
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/42—Liquid level
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00—Use of specific compounds during water treatment
    • C02F2305/08—Nanoparticles or nanotubes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/10—Process efficiency
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/30—Wastewater or sewage treatment systems using renewable energies
    • Y02W10/37—Wastewater or sewage treatment systems using renewable energies using solar energy

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Thermal Sciences (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Ecology (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention belongs to the technical field of bacterium-algae decontamination and oil production, and discloses a bacterium-algae coupling integrated device and an oil production decontamination method, wherein the bacterium-algae coupling integrated device comprises the following steps: installing a reactor, and preparing suspension and nano particles; adding water into the bacteria-algae coupling integrated equipment, inoculating a proper amount of suspension, concentrating algae liquid, adding a certain amount of nano particles, an active oxygen inducer and a carbon fixation enhancer, and culturing bacteria-algae coupling particles; adding alkaline desorption solution into a reactor, and performing bacteria-algae regeneration based on the bacteria-algae coupling particles; and taking out mature bacteria and algae, drying to obtain dry bacteria, and calculating the oil yield based on the dry bacteria. The invention can degrade the sewage to make the sewage discharge up to the standard, and simultaneously greatly improves the yield of the mature microalgae refined bio-oil, and produces products with high added value. The aim of emission reduction is achieved in the middle treatment process, the production of the bio-oil is realized, and the operation cost is reduced.

Description

Bacteria-algae coupling integrated equipment and oil production and decontamination method
Technical Field
The invention belongs to the technical field of bacterium-algae decontamination and oil production, and particularly relates to bacterium-algae coupling integrated equipment and an oil production decontamination method.
Background
Currently, the algal-bacterial symbiotic system (algal-bacterial system) is a fresh water ecosystem that purifies sewage by the physiological functional synergy between algae and bacteria. Algae plants utilize CO in water through photosynthesis2And NH4+、PO4 3-Iso-nutrients, synthesize self-cellular material and release O2(ii) a The aerobic bacteria utilize O in the water2Decomposing and converting organic pollutants to generate CO2And the nutrient substances are used for maintaining the growth and the propagation of the algae, and the steps are repeated in a circulating way to realize the biological purification effect of the sewage. The efficiency of the algae symbiotic system for treating sewage depends on various factors such as solar energy radiation quantity, temperature, pollution degree (load and toxicity) and retention time.
The prior patent CN108516618A is an immobilized microalgae reactor using mycelium pellets as a carrier and a wastewater treatment method, wherein the main part of the reactor and a microalgae symbiotic system are mentioned, but the problems of energy source and the like of the reactor are not clearly suggested. Patent CN212396694U is a photocatalysis nitrogen fixation reactor, wherein the problem that the contact area of reactant and photocatalyst existing in the existing equipment is small, and the catalyst is difficult to recover is solved, and the LED lamp is used as the energy source, but the electrical appliance energy consumption is too much, and the cost is high. CN111924929A is a photocatalysis reactor for treating waste water and waste gas, which comprises a mounting plate, a catalytic tube is arranged above the mounting plate, the exterior of the catalytic tube is uniformly provided with a lamp tube along the circumferential direction of the catalytic tube, which can meet the requirements of photocatalysis, but the reactor has a more complex structure and higher manufacturing cost. Meanwhile, the traditional sewage purification process mostly takes activated sludge as a center, but the activated sludge cannot well utilize a large amount of resources such as nitrogen, phosphorus and the like in the sewage. The traditional activated sludge method has the defects of large occupied area, need of adding a reflux device, high energy and power consumption, high treatment cost of excess sludge, need of adding an organic matrix and the like.
(1) The traditional sewage purification process mostly takes activated sludge as a center, but the activated sludge cannot well utilize a large amount of resources such as nitrogen, phosphorus and the like in the sewage.
(2) The traditional activated sludge method has the defects of large occupied area, need of adding a reflux device, high energy and power consumption, high treatment cost of excess sludge, need of adding an organic matrix and the like.
(3) The energy consumption of the reactor is excessive, the structure of the reactor is complex, and the manufacturing cost is high.
The difficulty in solving the above problems and defects is: the growth environment of the mycelial pellets and the microalgae is influenced by a plurality of factors, the regulation and control of reaction conditions are difficult, and the increase of the content of the lipid is stimulated by the lack of nitrogen, and the increase of the content of the lipid is accompanied by the stagnation of the cell growth and the reduction of biomass, so that the lipid yield cannot be effectively improved. Thus, high oil content and high oil yield are often difficult to achieve simultaneously.
The significance of solving the problems and the defects is as follows: the mycelium pellet and the microalgae are cultured together to form a symbiotic system, so that respective advantages are exerted, and a new method and a new idea are provided for a sewage treatment technology. Meanwhile, the problems of long reaction time and poor reaction stability of bacteria and algae are solved. The development of renewable eco-friendly fuels is of great significance for maintaining good ecological environment and relieving energy crisis.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides bacteria-algae coupling integrated equipment and an oil production decontamination method.
The invention is realized in such a way that a bacteria-algae coupling integrated oil production decontamination method comprises the following steps:
step one, installing a reactor, and preparing suspension and nano particles; adding water into the bacteria-algae coupling integrated equipment;
inoculating a certain amount of prepared suspension at an opening at the upper end of a reactor main body of the bacteria-algae coupling integrated equipment, adding a certain amount of nano particles, an active oxygen inducer and a carbon fixation enhancer, and culturing bacteria-algae coupling particles;
step three, adding NaOH solution serving as alkaline desorption solution into the reactor, and performing bacteria-algae regeneration based on the bacteria-algae coupling particles; and taking out mature bacteria and algae, drying to obtain dry bacteria, and calculating the oil yield based on the dry bacteria.
Further, the preparing comprises: preparing fungal spore suspension, mycelial ball suspension or broken mycelial suspension;
preparing a fungal spore suspension: transferring the fungal spores on the inclined plane into sterile water filled with glass beads, suspending the spores on the inclined plane in the sterile water, placing the suspension with the glass beads in a shaking table at 180rpm and shaking for 2 hours to fully disperse the spores, wherein the spore content in each milliliter is 108～109cfu；
Preparing a mycelium pellet suspension: inoculating the spore suspension into a bacterium-algae coupling culture medium, wherein the concentration of the inoculated spores is 104The strain per mL is 1mL/1000mL, the initial pH of the culture medium is 6-7, and the culture is carried out for 3d on a shaker at 37 ℃ and 160 rpm;
preparation of broken mycelium suspension: the mycelium pellet spore is prepared by selecting fungus Aspergillus niger, transferring cultured mature mycelium pellet into sterile water filled with glass beads, placing in a shaking table with 180rpm for 30min, crushing with a stirrer for 30s, and inoculating the obtained mycelium fragment into a strain-algae coupling culture medium for culture.
Further, in the first step, the nanoparticle preparation method comprises:
mixing SiO2The particles and MWCNTs were mixed in a molar ratio of 1:14 and thoroughly mixed by milling for at least 1 h; placing the mixture in a corundum crucible, calcining in a sintering furnace at 1500 ℃, and taking 10L/h of argon as a protective gas; after sintering is finishedThe resulting main product was cooled to room temperature, then heated to 700 ℃ under a stream of air at a rate of 5 ℃/min for 8 h.
Further, in the first step, the step of adding water into the bacteria-algae coupling integrated equipment comprises:
and (3) under the condition of 20-30 ℃, simulated sewage in the water inlet tank is poured into a descending pipe of the reactor main body through a water inlet pipe by using a water inlet pump, the water inlet time of the water inlet pump is set to be 3-6 min through a non-negative pressure frequency conversion device, and the water inlet pump is closed after water inlet is finished.
Further, in the second step, the inoculation and addition of the suspension, the concentrated algae solution, the nanoparticles, the active oxygen inducer and the carbon fixation enhancer comprise:
the volume ratio of the addition amount of the fungal spore suspension to the sewage of the reactor is 1: 1000; the final inoculation concentration of the mycelium pellet suspension is 400mg/L, the final inoculation concentration of the broken mycelium suspension is 200mg/L, the final addition amount of the carbon fixation enhancer is 300mg/L, and the volume ratio of the addition amount of the concentrated algae liquid to the sewage of the reactor is 1: 10;
the addition amount of the nano particles is 150 mg/L; the adding amount of the active oxygen inducer is 1 mmol/L; the active oxygen inducer is a sodium copper chlorophyllin solution;
the carbon fixation reinforcer consists of methanol carbonic acid and propylene ester; the concentration of methanol in the carbon fixation reinforcer is 0.05-1.0%, and the concentration of propylene ester in the carbon fixation reinforcer is 0.5-10 mmol/L.
Further, in the second step, the cultured bacteria-algae coupling particles comprise:
air is supplied into the reactor main body from the aeration head through the air inlet pipe, so that sewage in the reactor main body moves upwards in the ascending pipe under the driving of ascending air flow, rises to the joint of the upper end of the ascending pipe and the upper end of the descending pipe, starts to enter the descending pipe, and moves downwards to the bottom of the reactor main body; the fungal spores are subjected to aeration culture for 12-60 hours under the conditions of 20-30 ℃, air ventilation capacity of 60-180L/h, dissolved oxygen of 3.0mg/L, pH 6-8, illumination intensity of 5000-10000 Lux and light-dark ratio of 12:12 to form green mycelium pellets.
Further, in the second step, after the bacteria and algae are cultured and coupled with the particles, the following steps are required:
after the bacteria and algae coupled particles are obtained, the aeration equipment is closed to separate the bacteria and algae coupled particles from the sewage under the gravity condition, the precipitation is carried out for 1min, the water is drained for 5min, and the reactor enters the idle period for 120 min; the bacteria and algae are left in the reactor and enter a stable operation period;
the steady operation period includes: the reactor adopts intermittent water feeding, and the operation conditions are as follows: the period is 12h, including water feeding in 5min, aeration for 589min, precipitation for 1min, water draining for 5min and standing for 120 min; the volume exchange rate of the reactor is 50 percent, and the hydraulic retention time is 12 hours.
Further, in the third step, the adding NaOH solution as an alkaline desorption solution into the reactor, and performing bacteria and algae regeneration based on the bacteria and algae coupled particles comprises:
adding 0.01mol/L NaOH solution serving as alkaline desorption solution into a reactor, aerating to uniformly distribute the mycelium pellets in the alkaline solution, desorbing the adsorbed substances into the alkaline solution, and recovering and storing the desorbed alkaline solution to obtain regenerated mycelium pellets.
Further, in the third step, the calculating the oil yield based on dry thalli includes:
adding 10mL of 4mol/L hydrochloric acid into 0.5g of dry thallus, uniformly oscillating, standing at room temperature for lh, then boiling in a water bath, standing at-20 ℃ after boiling in the water bath, rapidly cooling for 30min, pouring into a separating funnel, adding 5mL of absolute ethanol, oscillating, adding 12mL of diethyl ether and 12mL of petroleum ether mixed organic solvent for extraction, collecting an organic layer in a weighed test tube, and recording the mass as m1(ii) a Heating to volatilize the organic solvent, weighing, and recording the mass as m2The oil yield was calculated using the following formula:
Figure BDA0003415664250000051
another object of the present invention is to provide a bacteria-algae coupling integrated apparatus for implementing the bacteria-algae coupling integrated oil-producing and decontamination method, wherein the bacteria-algae coupling integrated apparatus is provided with:
a reactor;
the reactor is provided with an upper fixing disc and a lower fixing disc which are connected through a support column; the upper and lower fixed disks are fixed with outer tubes which are connected with a water pump through a water inlet pipe; the water pump is connected with one end of the non-negative pressure variable-frequency water supply device, and the other end of the non-negative pressure variable-frequency water supply device is connected with the water pump;
the inner pipe is arranged in the outer pipe, the aeration head is arranged at the bottom side of the inner pipe, and the aeration head is connected with an air compressor through an air pipe; the upper side of the outer pipe is provided with a sampling port, and the inner part of the inner pipe is provided with a sensor;
the sensor and the air compressor are respectively connected with the PLC through data lines, the sensor and the air compressor are respectively connected with the storage battery through conducting wires, the storage battery is connected with the solar cell panel, and the solar cell panel receives an external light source;
the sensor includes: a temperature controller, a COD sensor, a pH sensor, a light sensation sensor, a dissolved oxygen sensor, a total nitrogen and total phosphorus sensor and a liquid level sensor.
By combining all the technical schemes, the invention has the advantages and positive effects that:
the main body part of the invention is the self photoproduction reaction of microalgae, scenedesmus with high oil content in the microalgae is selected, mycelium pellets are used as growth carriers of scenedesmus to form a scenedesmus symbiotic system, pollutants such as nitrogen, phosphorus and the like in sewage are immobilized, and biological grease is generated through the biochemical reaction of the microalgae for collection, and the biological grease can be used as a raw material of biodiesel for recycling. On the basis, a temperature sensor, a pH sensor, a light sensation sensor and a dissolved oxygen sensor are arranged in the reactor, a designed PLC circuit is used for controlling water outlet and operation management of the whole reaction system, water inlet is controlled through a non-negative-pressure variable-frequency water supply device, primary water supply pressure of a tap water pipe network is fully utilized, equipment investment is reduced, the optimal conditions are determined through experiments, the circuit system is used for automatic control, labor cost is saved, and the problems that the traditional activated sludge method is large in occupied area, a backflow device needs to be added, energy and power consumption are high, the cost of residual sludge treatment is high, organic substrates need to be added and the like are solved.
According to the invention, reaction equipment is reasonably constructed on the basis of mycelial ball-microalgae reaction, and the nano particles and the active oxygen inducer are added in the mycelial ball-microalgae reaction process, so that the sewage can be degraded to reach the standard and discharged, and meanwhile, the yield of the mature microalgae refined bio-oil is greatly improved, and a high value-added product is produced. The aim of emission reduction is achieved in the middle treatment process, the production of the bio-oil is realized, and the operation cost is reduced.
The solar energy collecting and converting device adopts the PLC circuit to carry out central control, and in addition, the solar panel is adopted to collect and convert the light energy, so that the electric energy required by the operation of a part of devices is reduced, and the waste of human resources is reduced; the intelligent environment-friendly device can run for a long time after being opened by one key, and software can be upgraded at any time if technical breakthrough is realized, so that the intelligent environment-friendly device is expected to be the pioneer of intelligent environment-friendly equipment. The equipment of the invention has less material consumption and low operation cost, and can be widely popularized and developed in the market.
The invention carries out water feeding through the non-negative pressure variable frequency water supply equipment, does not need to build a reservoir or a water tank, fully utilizes the primary water supply pressure of a tap water pipe network, reduces the equipment investment, directly connects the equipment with the tap water pipe network in series, can fully utilize the excess pressure, can not stop water supply when the water supply amount is insufficient, can not stop water supply when power failure occurs, can not generate pollution, and is simple to maintain and manage.
The invention provides an effective method for controlling the discharge of nitrogen and phosphorus in simulated wastewater (domestic wastewater), is widely applied to the synchronous removal of nitrogen and phosphorus in domestic wastewater, and has good environmental benefit.
According to the invention, nanoparticles and an active oxygen inducer are added in the reaction process, and the synergistic effect of the nanoparticles and the active oxygen inducer can promote the growth of bacteria and algae and produce oil.
The nano particles are made of SiC, are attached to the surfaces of the microalgae, and photo-generated holes and photo-generated electrons generated by photocatalysis can play a certain role in promoting the metabolic synthesis of the microalgae, or the generated photo-generated electrons generate a certain relation to an electron transfer system of the microalgae, so that the catalytic activity of the acetyl coenzyme A enzyme is improved.
The active oxygen inducer adopts sodium copper chlorophyllin solution, and sodium copper chlorophyllin is sodium copper chlorophyllinThe active oxygen inducer can generate singlet oxygen (O) under illumination condition2) Or a superoxide anion (O)2 -) Wherein the superoxide anion (O)2 +) Can be further reacted to produce H2O2Or hydroxyl radical (OH)-)。O2Is an oxidant with strong reactivity and can react with electron-rich substances quickly to generate H2O2It can enhance the activity of enzyme and promote oil production by bacteria and algae.
The carbon fixation enhancer disclosed by the invention consists of methanol carbonate and propylene ester, contains hydroxyl and ester substances, and can enhance the process of utilizing light reaction of bacteria and algae to generate ATP and NADPH to synthesize organic matters.
Drawings
Fig. 1 is a flow chart of a bacteria-algae coupling integrated oil production and decontamination method provided by an embodiment of the invention.
Fig. 2 is a schematic diagram of an integrated oil production and decontamination process by bacteria-algae coupling provided by an embodiment of the invention.
FIG. 3 is a schematic structural diagram of an integrated bacteria-algae coupling apparatus provided in an embodiment of the present invention;
in fig. 3: 1. a water inlet pipe; 2. a sampling port; 3. a sensor; 4. a water inlet pump; 5. a valve; 6. an aeration head; 7. an inner tube; 8. an outer tube; 9. a support pillar; 10. an air compressor; 11. a storage battery; 12. a solar panel; 13. a PLC controller; 14. an external light source; 15. non-negative pressure variable frequency water supply equipment; FIG. 3(a) is a structural overall view of an integrated bacteria-algae coupling apparatus; FIG. 3(b) is a schematic view showing the connection of the inner tube, the outer tube and the support pillar; fig. 3(c) is a schematic view of the valve 5. FIG. 3(d) is a side view of the upper and lower fixing disks.
FIG. 4 is a diagram of COD data processing provided by the embodiment of the present invention.
FIG. 5 is a diagram of ammonia nitrogen data processing provided by the embodiment of the present invention.
FIG. 6 is a graph of total phosphorus data processing provided by an embodiment of the present invention.
Fig. 7 is a diagram of processing grease data according to an embodiment of the present invention.
Fig. 8 is a flow chart of water inflow provided by an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides bacteria-algae coupling integrated equipment and an oil production decontamination method, and the invention is described in detail below with reference to the accompanying drawings.
As shown in fig. 1, the bacteria-algae coupling integrated oil production and decontamination method provided by the embodiment of the present invention includes:
s101, installing a reactor, and preparing suspension and nano particles; adding water into the bacteria-algae coupling integrated equipment;
s102, inoculating a certain amount of prepared suspension at an opening at the upper end of a reactor main body of the bacteria-algae coupling integrated equipment, adding a certain amount of nano particles, an active oxygen inducer and a carbon fixation enhancer, and culturing bacteria-algae coupling particles;
s103, adding NaOH solution serving as alkaline desorption solution into the reactor, and performing bacteria-algae regeneration based on the bacteria-algae coupling particles; and taking out mature bacteria and algae, drying to obtain dry bacteria, and calculating the oil yield based on the dry bacteria.
The preparation of the suspension provided by the embodiment of the invention comprises the following steps: preparing fungal spore suspension, mycelial ball suspension or broken mycelial suspension;
preparing a fungal spore suspension: transferring the fungal spores on the inclined plane into sterile water filled with glass beads, suspending the spores on the inclined plane in the sterile water, placing the suspension with the glass beads in a shaking table at 180rpm and shaking for 2 hours to fully disperse the spores, wherein the spore content in each milliliter is 108～109cfu；
Preparing a mycelium pellet suspension: inoculating the spore suspension into a bacterium-algae coupling culture medium, wherein the concentration of the inoculated spores is 104The strain per mL is 1mL/1000mL, the initial pH of the culture medium is 6-7, and the culture is carried out for 3d on a shaker at 37 ℃ and 160 rpm;
preparation of broken mycelium suspension: the mycelium pellet spore is prepared by selecting fungus Aspergillus niger, transferring cultured mature mycelium pellet into sterile water filled with glass beads, placing in a shaking table with 180rpm for 30min, crushing with a stirrer for 30s, and inoculating the obtained mycelium fragment into a strain-algae coupling culture medium for culture.
The preparation method of the nano-particles provided by the embodiment of the invention comprises the following steps:
mixing SiO2The particles and MWCNTs were mixed in a molar ratio of 1:14 and thoroughly mixed by milling for at least 1 h; placing the mixture in a corundum crucible, calcining in a sintering furnace at 1500 ℃, and taking 10L/h of argon as a protective gas; after sintering was complete, the resulting main product was cooled to room temperature, then heated to 700 ℃ under a stream of air at a rate of 5 ℃/min for 8 h.
The embodiment of the invention provides a method for adding water into bacteria-algae coupling integrated equipment, which comprises the following steps:
and (3) under the condition of 20-30 ℃, simulated sewage in the water inlet tank is poured into a descending pipe of the reactor main body through a water inlet pipe by using a water inlet pump, the water inlet time of the water inlet pump is set to be 3-6 min through a non-negative pressure frequency conversion device, and the water inlet pump is closed after water inlet is finished.
The inoculation and addition of the suspension, the concentrated algae solution, the nano particles, the active oxygen inducer and the carbon fixation enhancer provided by the embodiment of the invention comprise:
the volume ratio of the addition amount of the fungal spore suspension to the sewage of the reactor is 1: 1000; the final inoculation concentration of the mycelium pellet suspension is 400mg/L, the final inoculation concentration of the broken mycelium suspension is 200mg/L, the final addition amount of the carbon fixation enhancer is 300mg/L, and the volume ratio of the addition amount of the concentrated algae liquid to the sewage of the reactor is 1: 10;
the addition amount of the nano particles is 150 mg/L; the adding amount of the active oxygen inducer is 1 mmol/L; the active oxygen inducer is a sodium copper chlorophyllin solution;
the carbon fixation reinforcer consists of methanol carbonic acid and propylene ester; the concentration of methanol in the carbon fixation reinforcer is 0.05-1.0%, and the concentration of propylene ester in the carbon fixation reinforcer is 0.5-10 mmol/L.
The embodiment of the invention provides a coupling particle for culturing bacteria and algae, which comprises:
air is supplied into the reactor main body from the aeration head through the air inlet pipe, so that sewage in the reactor main body moves upwards in the ascending pipe under the driving of ascending air flow, rises to the joint of the upper end of the ascending pipe and the upper end of the descending pipe, starts to enter the descending pipe, and moves downwards to the bottom of the reactor main body; the fungal spores are subjected to aeration culture for 12-60 hours under the conditions of 20-30 ℃, air ventilation capacity of 60-180L/h, dissolved oxygen of 3.0mg/L, pH 6-8, illumination intensity of 5000-10000 Lux and light-dark ratio of 12:12 to form green mycelium pellets.
The cultivation of bacteria and algae coupled particles provided by the embodiment of the invention needs to be carried out as follows:
after the bacteria and algae coupled particles are obtained, the aeration equipment is closed to separate the bacteria and algae coupled particles from the sewage under the gravity condition, the precipitation is carried out for 1min, the water is drained for 5min, and the reactor enters the idle period for 120 min; the bacteria and algae are left in the reactor and enter a stable operation period;
the stable operation period provided by the embodiment of the invention comprises the following steps: the reactor adopts intermittent water feeding, and the operation conditions are as follows: the period is 12h, including water feeding in 5min, aeration for 589min, precipitation for 1min, water draining for 5min and standing for 120 min; the volume exchange rate of the reactor is 50 percent, and the hydraulic retention time is 12 hours.
The method for adding NaOH solution into a reactor as alkaline desorption solution and regenerating bacteria and algae based on the bacteria and algae coupling particles comprises the following steps:
adding 0.01mol/L NaOH solution serving as alkaline desorption solution into a reactor, aerating to uniformly distribute the mycelium pellets in the alkaline solution, desorbing the adsorbed substances into the alkaline solution, and recovering and storing the desorbed alkaline solution to obtain regenerated mycelium pellets.
The method for calculating the oil yield based on the dry thalli provided by the embodiment of the invention comprises the following steps:
adding 10mL of 4mol/L hydrochloric acid into 0.5g of dry thallus, oscillating, mixing, standing at room temperature for lh, boiling in water bath, standing at-20 deg.C in boiling water bath, rapidly cooling for 30min, pouring into a separating funnel, adding 5mL of anhydrous ethanol, oscillating, adding 12mL of diethyl ether and 1mL of anhydrous ethanol, and mixingExtracting with 2mL petroleum ether mixed organic solvent, collecting organic layer in weighed test tube, and recording mass as m1(ii) a Heating to volatilize the organic solvent, weighing, and recording the mass as m2The oil yield was calculated using the following formula:
Figure BDA0003415664250000101
as shown in fig. 3, the bacteria-algae coupling integrated apparatus provided by the embodiment of the present invention is provided with a reactor, and the reactor is provided with upper and lower fixed disks connected by a support column 9.
An outer pipe 8 is fixed on the upper fixing disc and the lower fixing disc, and the outer pipe 8 is connected with a water pump 4 through a water inlet pipe 1; an inner pipe 7 is arranged in the outer pipe 8, an aeration head 6 is arranged at the bottom side of the inner pipe 7, and the aeration head 6 is connected with an air compressor 10 through an air pipe. The upper side of the outer tube 8 is provided with a sampling port 2, and the inner tube 7 is internally provided with a sensor 3.
The sensor 3 and the air compressor 10 are respectively connected with the PLC 13 through data lines, the sensor 3 and the air compressor 10 are respectively connected with the storage battery 11 through conducting wires, the storage battery 11 is connected with the solar cell panel 12, and the solar cell panel 12 receives the external light source 14. The lower end of the lower fixed disc is provided with a valve 5.
The water pump 4 is connected with one end of the non-negative pressure variable frequency water supply equipment 15, and the other end of the non-negative pressure variable frequency water supply equipment 15 is connected with a tap water pipe network in series.
The sensor 3 includes: a temperature controller, a COD sensor, a pH sensor, a light sensation sensor, a dissolved oxygen sensor, a total nitrogen and total phosphorus sensor and a liquid level sensor.
The water inlet pipe 1 is connected with the water pump 4 to facilitate the raw water to enter, and the sampling ports 2 are positioned at the two sides of the reactor to facilitate the sampling observation at any time; the water outlet pipe and the sludge discharge port of the outer pipe 8 are positioned at one position and are controlled by a valve; the outer side wall of the outer pipe 8 is provided with a water outlet pipe, and the water outlet pipe is connected with a water outlet tank. The aeration head 6 is positioned at the bottom of the reactor and is connected with an air compressor 10 to aerate the reaction.
Reactor parameters: capacity of an outer pipe: 16L, inner tube capacity: 6L; the height of the outer pipe is as follows: 90cm, inner tube height: 80cm, inner diameter of outer tube: 16cm, inner diameter of inner tube: 10cm, liquid level monitor at 70cm of the outer wall of the inner tube of the reactor, operating cycle: 12h, reaction column material: acrylic material.
The effects of the present invention will be further described with reference to the experimental results.
According to the bacteria-algae coupling integrated equipment and the oil production decontamination method, the removal rate of bacteria-algae COD, ammonia nitrogen and total phosphorus and the oil yield are shown in the figures 4, 5, 6 and 7, the removal effect is good, and the oil production yield is high.
Fig. 8 is a flow chart of water inflow provided by an embodiment of the present invention.
In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.

Claims (10)

1. The bacteria-algae coupling integrated oil production decontamination method is characterized by comprising the following steps:
step one, installing a reactor, and preparing suspension and nano particles; adding water into the bacteria-algae coupling integrated equipment;
inoculating a certain amount of prepared suspension at an opening at the upper end of a reactor main body of the bacteria-algae coupling integrated equipment, adding a certain amount of nano particles, an active oxygen inducer and a carbon fixation enhancer, and culturing bacteria-algae coupling particles;
step three, adding NaOH solution serving as alkaline desorption solution into the reactor, and performing bacteria-algae regeneration based on the bacteria-algae coupling particles; and taking out mature bacteria and algae, drying to obtain dry bacteria, and calculating the oil yield based on the dry bacteria.
2. The bacteria-algae coupling integrated oil production and decontamination method of claim 1, wherein the preparing the suspension comprises: preparing fungal spore suspension, mycelial ball suspension or broken mycelial suspension;
preparing a fungal spore suspension: transferring the fungal spores on the inclined plane into sterile water filled with glass beads, suspending the spores on the inclined plane in the sterile water, placing the suspension with the glass beads in a shaking table at 180rpm and shaking for 2 hours to fully disperse the spores, wherein the spore content in each milliliter is 108～109cfu；
Preparing a mycelium pellet suspension: inoculating the spore suspension into a bacterium-algae coupling culture medium, wherein the concentration of the inoculated spores is 104The strain per mL is 1mL/1000mL, the initial pH of the culture medium is 6-7, and the culture is carried out for 3d on a shaker at 37 ℃ and 160 rpm;
preparation of broken mycelium suspension: the mycelium pellet spore is prepared by selecting fungus Aspergillus niger, transferring cultured mature mycelium pellet into sterile water filled with glass beads, placing in a shaking table with 180rpm for 30min, crushing with a stirrer for 30s, and inoculating the obtained mycelium fragment into a strain-algae coupling culture medium for culture.
3. The bacteria-algae coupling integrated oil production and decontamination method of claim 1, wherein in the first step, the nanoparticle preparation method comprises:
mixing SiO2The particles and MWCNTs are mixed in a molar ratio of 1:14 and milled for at least 1h toFully and uniformly mixing; placing the mixture in a corundum crucible, calcining in a sintering furnace at 1500 ℃, and taking 10L/h of argon as a protective gas; after sintering was complete, the resulting main product was cooled to room temperature, then heated to 700 ℃ under a stream of air at a rate of 5 ℃/min for 8 h.
4. The bacteria-algae coupling integrated oil production and decontamination method of claim 1, wherein in the first step, the step of adding water into the bacteria-algae coupling integrated equipment comprises:
and (3) under the condition of 20-30 ℃, simulated sewage in the water inlet tank is poured into a descending pipe of the reactor main body through a water inlet pipe by using a water inlet pump, the water inlet time of the water inlet pump is set to be 3-6 min through a non-negative pressure frequency conversion device, and the water inlet pump is closed after water inlet is finished.
5. The bacteria-algae coupling integrated oil production and decontamination method of claim 1, wherein in the second step, the inoculation and addition of the suspension, the concentrated algae solution, the nanoparticles, the active oxygen inducer and the carbon fixation enhancer comprises:
the volume ratio of the addition amount of the fungal spore suspension to the sewage of the reactor is 1: 1000; the final inoculation concentration of the mycelium pellet suspension is 400mg/L, the final inoculation concentration of the broken mycelium suspension is 200mg/L, the final addition amount of the carbon fixation enhancer is 300mg/L, and the volume ratio of the addition amount of the concentrated algae liquid to the sewage of the reactor is 1: 10;
the addition amount of the nano particles is 150 mg/L; the adding amount of the active oxygen inducer is 1 mmol/L; the active oxygen inducer is a sodium copper chlorophyllin solution;
the carbon fixation reinforcer consists of methanol carbonic acid and propylene ester; the concentration of methanol in the carbon fixation reinforcer is 0.05-1.0%, and the concentration of propylene ester in the carbon fixation reinforcer is 0.5-10 mmol/L.
6. The bacteria-algae coupling integrated oil production and decontamination method of claim 1, wherein in the second step, the culturing of the bacteria-algae coupling particles comprises:
air is supplied into the reactor main body from the aeration head through the air inlet pipe, so that sewage in the reactor main body moves upwards in the ascending pipe under the driving of ascending air flow, rises to the joint of the upper end of the ascending pipe and the upper end of the descending pipe, starts to enter the descending pipe, and moves downwards to the bottom of the reactor main body; the fungal spores are subjected to aeration culture for 12-60 hours under the conditions of 20-30 ℃, air ventilation capacity of 60-180L/h, dissolved oxygen of 3.0mg/L, pH 6-8, illumination intensity of 5000-10000 Lux and light-dark ratio of 12:12 to form green mycelium pellets.
7. The bacteria-algae coupling integrated oil production and decontamination method of claim 1, wherein in the second step, the cultivation of the bacteria-algae coupling particles is followed by:
after the bacteria and algae coupled particles are obtained, the aeration equipment is closed to separate the bacteria and algae coupled particles from the sewage under the gravity condition, the precipitation is carried out for 1min, the water is drained for 5min, and the reactor enters the idle period for 120 min; the bacteria and algae are left in the reactor and enter a stable operation period;
the steady operation period includes: the reactor adopts intermittent water feeding, and the operation conditions are as follows: the period is 12h, including water feeding in 5min, aeration for 589min, precipitation for 1min, water draining for 5min and standing for 120 min; the volume exchange rate of the reactor is 50 percent, and the hydraulic retention time is 12 hours.
8. The bacteria-algae coupling integrated oil production and decontamination method of claim 1, wherein in the third step, the NaOH solution is added into the reactor as an alkaline desorption solution, and the bacteria-algae regeneration based on the bacteria-algae coupling particles comprises:
adding 0.01mol/L NaOH solution serving as alkaline desorption solution into a reactor, aerating to uniformly distribute the mycelium pellets in the alkaline solution, desorbing the adsorbed substances into the alkaline solution, and recovering and storing the desorbed alkaline solution to obtain regenerated mycelium pellets.
9. The integrated oil-producing and stain-removing method of claim 1, wherein in step three, the calculating the oil yield based on dry bacteria comprises:
0.5g of dry thallus is taken and added with 4mol/L hydrochloric acid10mL, shaking and mixing uniformly, standing at room temperature for lh, then boiling in a water bath, then placing at-20 ℃ for rapidly cooling for 30min, pouring into a separating funnel, adding 5mL of absolute ethanol for shaking, adding 12mL of diethyl ether and 12mL of petroleum ether mixed organic solvent for extraction, collecting an organic layer in a weighed test tube, and recording the mass as m1(ii) a Heating to volatilize the organic solvent, weighing, and recording the mass as m2The oil yield was calculated using the following formula:
Figure FDA0003415664240000031
10. a bacteria-algae coupling integrated device for implementing the bacteria-algae coupling integrated oil production and decontamination method according to any one of claims 1-9, wherein the bacteria-algae coupling integrated device is provided with:
a reactor;
the reactor is provided with an upper fixing disc and a lower fixing disc which are connected through a support column; the upper and lower fixed disks are fixed with outer tubes which are connected with a water pump through a water inlet pipe; the water pump is connected with one end of the non-negative pressure variable-frequency water supply device, and the other end of the non-negative pressure variable-frequency water supply device is connected with the water pump;
the inner pipe is arranged in the outer pipe, the aeration head is arranged at the bottom side of the inner pipe, and the aeration head is connected with an air compressor through an air pipe; the upper side of the outer pipe is provided with a sampling port, and the inner part of the inner pipe is provided with a sensor;
the sensor and the air compressor are respectively connected with the PLC through data lines, the sensor and the air compressor are respectively connected with the storage battery through conducting wires, the storage battery is connected with the solar cell panel, and the solar cell panel receives an external light source;
the sensor includes: a temperature controller, a COD sensor, a pH sensor, a light sensation sensor, a dissolved oxygen sensor, a total nitrogen and total phosphorus sensor and a liquid level sensor.
CN202111561896.7A 2021-12-16 2021-12-16 Fungus-algae coupling integrated equipment and oil production and decontamination method Active CN114231406B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111561896.7A CN114231406B (en) 2021-12-16 2021-12-16 Fungus-algae coupling integrated equipment and oil production and decontamination method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111561896.7A CN114231406B (en) 2021-12-16 2021-12-16 Fungus-algae coupling integrated equipment and oil production and decontamination method

Publications (2)

Publication Number Publication Date
CN114231406A true CN114231406A (en) 2022-03-25
CN114231406B CN114231406B (en) 2023-02-28

Family

ID=80759134

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111561896.7A Active CN114231406B (en) 2021-12-16 2021-12-16 Fungus-algae coupling integrated equipment and oil production and decontamination method

Country Status (1)

Country Link
CN (1) CN114231406B (en)

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102491445A (en) * 2011-12-27 2012-06-13 南京林业大学 Multifunctional water treatment device by integrating heavy metal removal function and organic pollution removal function
CN103112993A (en) * 2013-01-31 2013-05-22 胜利油田胜利勘察设计研究院有限公司 Method for processing oilfield wastewater and fixing CO2 (carbon dioxide) by using microalgae
CN103757061A (en) * 2014-01-10 2014-04-30 武汉科技大学 Method for synthesizing chiral alcohol by utilizing asymmetric reduction of microalgae
CN203821483U (en) * 2014-02-12 2014-09-10 沈阳中晨泵业成套设备制造有限公司 Membrane treatment device of non-negative pressure water supply equipment
CN104987106A (en) * 2015-07-10 2015-10-21 常州市荣顺电子有限公司 Preparation method of biological microporous silicon carbide
CN106318620A (en) * 2016-08-15 2017-01-11 中国石油大学(华东) Preparation and application of microalgae natural dye
CN106552593A (en) * 2016-11-30 2017-04-05 中冶华天工程技术有限公司 Mycelium pellet biological adsorption and renovation process
CN108516618A (en) * 2018-05-10 2018-09-11 黑龙江科技大学 It is a kind of using mycelium pellet as the immobilized microalgae reactor and wastewater treatment method of carrier
CN110304734A (en) * 2019-06-17 2019-10-08 大连理工大学 A kind of cultivation tail water New Method for Processing for strengthening removal of inorganic nitrogen using bacterium algae-catalysis electrode
US20190322875A1 (en) * 2018-04-20 2019-10-24 Redjak, L.L.C. Methods and coatings for protecting surfaces from bio-fouling species
CN110484451A (en) * 2019-08-06 2019-11-22 天津农学院 A method of promoting haematococcus pluvialis growing and accumulation astaxanthin

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102491445A (en) * 2011-12-27 2012-06-13 南京林业大学 Multifunctional water treatment device by integrating heavy metal removal function and organic pollution removal function
CN103112993A (en) * 2013-01-31 2013-05-22 胜利油田胜利勘察设计研究院有限公司 Method for processing oilfield wastewater and fixing CO2 (carbon dioxide) by using microalgae
CN103757061A (en) * 2014-01-10 2014-04-30 武汉科技大学 Method for synthesizing chiral alcohol by utilizing asymmetric reduction of microalgae
CN203821483U (en) * 2014-02-12 2014-09-10 沈阳中晨泵业成套设备制造有限公司 Membrane treatment device of non-negative pressure water supply equipment
CN104987106A (en) * 2015-07-10 2015-10-21 常州市荣顺电子有限公司 Preparation method of biological microporous silicon carbide
CN106318620A (en) * 2016-08-15 2017-01-11 中国石油大学(华东) Preparation and application of microalgae natural dye
CN106552593A (en) * 2016-11-30 2017-04-05 中冶华天工程技术有限公司 Mycelium pellet biological adsorption and renovation process
US20190322875A1 (en) * 2018-04-20 2019-10-24 Redjak, L.L.C. Methods and coatings for protecting surfaces from bio-fouling species
CN108516618A (en) * 2018-05-10 2018-09-11 黑龙江科技大学 It is a kind of using mycelium pellet as the immobilized microalgae reactor and wastewater treatment method of carrier
CN110304734A (en) * 2019-06-17 2019-10-08 大连理工大学 A kind of cultivation tail water New Method for Processing for strengthening removal of inorganic nitrogen using bacterium algae-catalysis electrode
CN110484451A (en) * 2019-08-06 2019-11-22 天津农学院 A method of promoting haematococcus pluvialis growing and accumulation astaxanthin

Also Published As

Publication number Publication date
CN114231406B (en) 2023-02-28

Similar Documents

Publication Publication Date Title
WO2020232767A1 (en) Energy circulating active convection oxygenating ecological floating island
CN105098217B (en) Three-diemsnional electrode photoelectric microbiological fuel cell reactor and the method for biogas upgrading synergy
CN107935162A (en) A kind of efficiently copper removal bioelectrochemistry water treatment system
CN106374127A (en) Drop aeration type microbial fuel cell
CN103183447A (en) Treatment method of piggery wastewater
CN204625422U (en) A kind of Sewage treatment systems
CN104045151B (en) A kind of in-situ biogas purified reaction device based on bioelectrochemistry principle and method
CN108585379A (en) A kind of apparatus and method improving organic wastewater with difficult degradation thereby treatment effect
CN110104874A (en) A kind of continuous flow wetland type microbiological fuel cell reactor sewage-treatment plant
CN105565473B (en) A kind of bacterium algae combination Bioelectrochemical device and its application
CN103708693A (en) Digestion system and method for sludge produced by urban sewage treatment
CN101314780A (en) Method for producing methyl hydride and electricity with solar energy
CN205115138U (en) Little aeration systems of constructed wetland solar energy intermittent type
CN114231406B (en) Fungus-algae coupling integrated equipment and oil production and decontamination method
CN103865957A (en) Method utilizing hydrogen-producing acetogens and electricigens to enhance biological hydrogen production efficiency
CN101007682A (en) Hydrogen making apparatus using high concentrated organic wastewater and its hydrogen making method
CN213596102U (en) Leachate treatment tank for anaerobic coupling ozone microbubbles
CN205170492U (en) Domestic sewage purification system of unpowered aeration
CN205141079U (en) Three -dimensional electrode photoelectricity microbiological fuel cell reactor
CN107892392A (en) The bacteria and algae film sewage reactor attemperator and method of a kind of low-carbon energy-saving
CN208413986U (en) The high load capacity bioreactor being exclusively used in sewage disposal system
CN114196534A (en) Carbon-based emission reduction of CO2Device and method for biologically synthesizing methane
CN210133989U (en) Solar electro-catalysis integrated sewage purification device
CN113666502A (en) Carbon-neutralized solar energy thermal reactor for sewage treatment
CN209010201U (en) A kind of Fenton's reaction tower of improvement

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right

Effective date of registration: 20230109

Address after: No. 12 Wenzhuang Road, Qiting Street, Yixing City, Wuxi City, Jiangsu Province, 214203

Applicant after: JIANGSU TONGYONG ENVIRONMENT ENGINEERING CO.,LTD.

Address before: 150080 1606, building 11, Gaoxi, Jinyu Lancheng community, Harbin, Heilongjiang Province

Applicant before: Li Lixin

TA01 Transfer of patent application right
GR01 Patent grant
GR01 Patent grant