CN114223461A - Device and method for measuring growth speed of edible fungus hyphae - Google Patents
Device and method for measuring growth speed of edible fungus hyphae Download PDFInfo
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- CN114223461A CN114223461A CN202210067253.5A CN202210067253A CN114223461A CN 114223461 A CN114223461 A CN 114223461A CN 202210067253 A CN202210067253 A CN 202210067253A CN 114223461 A CN114223461 A CN 114223461A
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- measuring
- sealing
- pipe body
- edible fungus
- tube body
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- 241000233866 Fungi Species 0.000 title claims abstract description 63
- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000007789 sealing Methods 0.000 claims abstract description 97
- 238000011081 inoculation Methods 0.000 claims abstract description 23
- 239000000463 material Substances 0.000 claims abstract description 14
- 238000005259 measurement Methods 0.000 claims description 11
- -1 polyethylene Polymers 0.000 claims description 9
- 239000004033 plastic Substances 0.000 claims description 7
- 229920003023 plastic Polymers 0.000 claims description 7
- 239000002361 compost Substances 0.000 claims description 6
- 239000004417 polycarbonate Substances 0.000 claims description 6
- 229920000139 polyethylene terephthalate Polymers 0.000 claims description 6
- 239000005020 polyethylene terephthalate Substances 0.000 claims description 6
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims description 3
- 239000004698 Polyethylene Substances 0.000 claims description 3
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 229920000515 polycarbonate Polymers 0.000 claims description 3
- 229920000573 polyethylene Polymers 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 1
- 239000000741 silica gel Substances 0.000 claims 1
- 229910002027 silica gel Inorganic materials 0.000 claims 1
- 238000002474 experimental method Methods 0.000 description 5
- 241001391944 Commicarpus scandens Species 0.000 description 2
- 230000001788 irregular Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 229920001296 polysiloxane Polymers 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B5/00—Measuring arrangements characterised by the use of mechanical techniques
- G01B5/02—Measuring arrangements characterised by the use of mechanical techniques for measuring length, width or thickness
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a device and a method for measuring the growth speed of edible fungus hyphae, and relates to the field of edible fungus experimental devices.A vent hole group is arranged at the lower part of a measuring tube body, a first inoculation reference line and a second inoculation reference line which are parallel to each other are arranged on the side wall of the upper part of the measuring tube body, and scale marks are carved on the side wall of the measuring tube body; the device for measuring the growth speed of the hypha of the edible fungus further comprises: the upper sealing element is inserted into the upper opening of the measuring pipe body, and is used for sealing the upper part of the measuring pipe body; and the lower sealing element is sleeved on the lower part of the measuring pipe body by a sealing sleeve and completely covers the vent hole group. According to the technical scheme, the scale marks are arranged on the tube wall, so that the growth speed of the edible fungus hyphae can be conveniently and rapidly measured, and 2 scale marks can record the growth speed of the hyphae in 2 directions, so that the data is more reliable; the volume is less than that of the traditional fungus stick, the storage space is saved, the fungus stick is conveniently placed in an incubator for cultivation, the cultivation material is saved, and the operation is easy.
Description
Technical Field
The invention relates to the field of edible fungus experimental devices, in particular to a device and a method for measuring the growth speed of edible fungus hyphae.
Background
At present, the growth speed of edible fungus hyphae is an important index for measuring the biological characteristics of an edible fungus variety, and has a great influence on the production cycle of the edible fungus, but the common means for measuring the edible fungus at the present stage is to draw lines on a fungus bag, and measure the edible fungus by using a stationery ruler in the growth process of the edible fungus, so that the following problems exist:
1. the fungus sticks are irregular, and the growth rate of the edible fungus hyphae is measured inaccurately
2. The diameter of the edible fungus stick is too large, and the growing speeds in multiple directions are different
3. The volume of the fungus stick is large, the material consumption in the experimental process is too much, the occupied space is large, and the experimental period is long
4. The experimental fungus stick is generally only provided with an opening at one end, and the bottom is not ventilated, so hyphae usually grow very slowly when growing to the half, even cannot grow to the bottom, the period is long, and the experiment failure can be caused.
In summary, it is an urgent need to solve the above-mentioned problem to design a device for measuring the growth rate of hyphae of edible fungi, which has reliable measurement data and records the growth rates of hyphae in multiple directions.
Disclosure of Invention
In order to overcome the problems in the related art, the disclosed embodiment of the invention provides a device for measuring the growth speed of edible fungus hyphae. The technical scheme is as follows:
according to a first aspect of the disclosed embodiment of the invention, the edible fungus hypha growth speed measuring device comprises a measuring tube body, wherein the lower part of the measuring tube body is provided with a vent hole group, the side wall of the upper part of the measuring tube body is provided with a first inoculation reference line and a second inoculation reference line which are parallel to each other, and the side wall of the measuring tube body is provided with scale marks;
the device for measuring the growth speed of the hypha of the edible fungus further comprises:
an upper seal member including a sealing plug inserted into the upper opening of the measurement pipe body for sealing the upper portion of the measurement pipe body;
and the lower sealing element comprises a sealing sleeve which is sleeved on the lower part of the measuring pipe body and completely covers the vent hole group.
In one embodiment, the upper seal further comprises:
the boss is arranged on the side surface of the sealing plug in a surrounding manner;
and the handheld part is arranged on the upper surface of the sealing plug.
In one embodiment, the sealing plug is a silicone sealing plug.
In one embodiment, the lower seal further comprises:
and the sealing ring is arranged at the opening of the sealing sleeve in a surrounding manner, and the sealing ring and the sealing sleeve are integrally formed.
In one embodiment, the sealing sleeve is a polyethylene or polypropylene plastic bag.
In one embodiment, the sealing ring is a rubber sealing ring.
In one embodiment, the measurement tube body is a PET polyethylene terephthalate tube body or a PC polycarbonate phenol tube body.
In one embodiment, the set of vent holes comprises:
the main vent hole is arranged at the bottommost part of the measuring pipe body;
the plurality of sub-air holes are arranged on the lower part of the measuring pipe body at equal angles by taking the main air hole as the center.
In one embodiment, the number of the plurality of sub-vents is 8-15.
According to a first aspect of the disclosed embodiments of the present invention, there is provided a measuring method suitable for the above-mentioned device for measuring the growth rate of edible fungus hyphae, the method comprising the following steps:
the method comprises the following steps: filling compost into the measuring pipe body until the capacity of the compost reaches the second inoculation reference line;
step two: covering an upper sealing element, inserting a sealing plug of the upper sealing element into an upper opening of the measuring pipe body, and tightly attaching the sealing plug to the side wall of the opening of the measuring pipe body due to certain elasticity of the sealing plug to realize the sealing of the upper opening of the measuring pipe body;
step three: the sealing sleeve of the lower sealing element is sleeved on the lower part of the measuring pipe body and completely wraps the air holes of the sub-sealing element, and the elasticity of the sealing ring material is utilized to be tightly attached to the side wall of the lower part of the measuring pipe body, so that the sealing of the lower air hole group of the measuring pipe body is realized;
step four: sterilizing the measuring tube body with the upper sealing element and the lower sealing element assembled;
step five: inoculating strains between the inoculation reference line I and the inoculation reference line II;
step six: culturing edible fungi under proper conditions;
step seven: and recording the growth length by contrasting the scale marks according to the arrival position of the edible fungus hypha in the growth process.
The technical scheme provided by the embodiment of the invention has the following beneficial effects:
1. the scale marks are arranged on the tube wall, so that the growth speed of the edible fungus hyphae can be conveniently and rapidly measured, and the 2 scale marks can record the growth speed of the hyphae in 2 directions, so that the data is more reliable;
2. the volume is smaller than that of the traditional fungus stick, the placing space is saved, the fungus stick is convenient to be placed in an incubator for culture, and the culture material is saved;
3. the material is plastic, and the filling culture material is not easy to break when being compacted.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the disclosure.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present disclosure and together with the description, serve to explain the principles of the disclosure.
FIG. 1 is an exploded view of an apparatus for measuring the growth rate of hyphae of an edible fungus according to the present invention;
FIG. 2 is a perspective view of an apparatus for measuring the growth rate of hyphae of an edible fungus according to the present invention;
FIG. 3 is a schematic view of the construction of a measurement tube body according to the invention;
figure 4 is a cross-sectional view of the sealing sleeve of the invention;
FIG. 5 is a flow chart of the steps of the method for measuring the growth rate of the hyphae of the edible fungi.
Reference numerals:
1. measuring tube body 201, main vent 202, sub-vent
3. Inoculation reference line I4, inoculation reference line II 5 and sealing element
501. Sealing plug 502, convex ring 503 and hand-held part
6. Sealing element 601, sealing sleeve 602 and sealing ring
2. Graduation line 6011, first jacket layer 6012, and second jacket layer
6013. Filter layer 6014 and air holes
Detailed Description
Reference will now be made in detail to the exemplary embodiments, examples of which are illustrated in the accompanying drawings. When the following description refers to the accompanying drawings, like numbers in different drawings represent the same or similar elements unless otherwise indicated. The implementations described in the exemplary embodiments below are not intended to represent all implementations consistent with the present disclosure. Rather, they are merely examples of apparatus and methods consistent with certain aspects of the present disclosure, as detailed in the appended claims.
The technical scheme provided by the embodiment of the invention relates to a device and a method for measuring the growth speed of edible fungus hyphae, in particular to the field of edible fungus experimental devices. In the related technology, the fungus sticks are irregular, and the growth speed of the edible fungus hyphae is measured inaccurately; the diameter of the edible fungus stick is too large, and the growth speeds in multiple directions are different; the volume of the fungus stick is large, the material consumption in the experiment process is too much, the occupied space is large, and the experiment period is long; the experimental fungus stick is generally only provided with an opening at one end, and the bottom is not ventilated, so hyphae usually grow very slowly when growing to the half, even cannot grow to the bottom, the period is long, and the experiment failure can be caused. Based on this, according to the edible fungus hypha growth speed measuring device provided by the technical scheme, the scale marks are arranged on the tube wall, so that the edible fungus hypha growth speed can be conveniently and rapidly measured, and 2 scale marks can record hypha growth speeds in 2 directions, so that the data is more reliable; the volume is smaller than that of the traditional fungus stick, the placing space is saved, the fungus stick is convenient to be placed in an incubator for culture, and the culture material is saved; the material is plastic, and the filling culture material is not easy to break when being compacted.
The first embodiment is as follows:
fig. 1 is an installation exploded view schematically illustrating an edible fungus hypha growth rate measuring device according to the technical scheme of the disclosure. As can be seen from fig. 1 to 4, the lower portion of the measuring tube body 101 is provided with a vent hole group, the upper side wall of the measuring tube body 101 is provided with a first inoculation reference line 3 and a second inoculation reference line 4 which are parallel to each other, and the side wall of the measuring tube body 101 is marked with a scale line 2; the device for measuring the growth speed of the hypha of the edible fungus further comprises: an upper seal member, which includes a sealing plug 501, the sealing plug 501 being inserted into the upper opening of the measurement pipe body 101 for sealing the upper portion of the measurement pipe body 101; and the lower sealing element comprises a sealing sleeve 601, the sealing sleeve 601 is sleeved on the lower part of the measuring pipe body 101 and completely covers the vent hole group, and microorganisms can be prevented from entering the measuring pipe body 101 after the sealing sleeve 601 is used for sleeving and sealing.
In the example, the inoculation reference line I3 and the inoculation reference line II 4 are used for filling culture materials into the body tube, and need to be spaced from the lowest surface of the upper sealing ring because the culture materials cannot be completely filled, firstly, space inoculation is needed, and secondly, air is needed for hypha growth after inoculation.
In one embodiment, the upper seal further comprises: a boss 502, wherein the boss 502 is arranged around the side surface of the sealing plug 501; and a hand-held portion 503, wherein the hand-held portion 503 is arranged on the upper surface of the sealing plug 501.
In one embodiment, the sealing plug 501 is a silicone sealing plug.
In one embodiment, the lower seal further comprises: the sealing ring 602 is arranged around the opening of the sealing sleeve 601, and the sealing ring 602 and the sealing sleeve 601 are integrally formed.
In an embodiment, the sealing sleeve 601 is a polyethylene plastic bag or a polypropylene plastic bag, and it should be further noted that the sealing sleeve 601 includes a first cover layer 6011 and a second cover layer 6012, a filter layer 6013 is disposed between the first cover layer 6011 and the second cover layer 6012, two or more air holes 6014 are disposed on both the first cover layer 6011 and the second cover layer 6012, during the growth of the strain, the main air holes 201 and the sub air holes 202 may be used for ventilation, and the air holes 6014 on the first cover layer 6011 and the second cover layer 6012 may be used for ventilation, and the filter layer between the first cover layer 6011 and the second cover layer 6012 may prevent microorganisms from entering the measuring tube body 101.
In one embodiment, the seal ring 602 is a rubber seal ring.
In one embodiment, the measurement tube body 101 is a PET polyethylene terephthalate tube or a PC polycarbonate phenol tube.
In one embodiment, the set of vent holes comprises: a main vent hole 201, the main vent hole 201 being provided at the bottommost portion of the measurement pipe body 101; and the plurality of sub-vent holes 202 are arranged at the lower part of the measuring pipe body 101 at equal angles by taking the main vent hole 201 as the center.
In one embodiment, the number of the plurality of sub-vents is 8-15, preferably 10.
Example two, as shown in fig. 5:
the device for measuring the growth speed of the hypha of the edible fungus in the embodiment is used in the experiment as follows:
step S01: filling compost into the measuring pipe body until the capacity of the compost reaches the second inoculation reference line 4;
step S02: covering an upper sealing element, inserting a sealing plug of the upper sealing element into an upper opening of the measuring pipe body, and tightly attaching the sealing plug to the side wall of the opening of the measuring pipe body due to certain elasticity of the sealing plug to realize the sealing of the upper opening of the measuring pipe body;
step S03: sleeving a sealing sleeve of a lower sealing element on the lower part of the measuring pipe body 101, completely wrapping the air holes, and tightly attaching the sealing sleeve to the side wall of the lower part of the measuring pipe body 101 by using the elasticity of the sealing ring material to realize the sealing of the lower air hole group of the measuring pipe body;
step S04: sterilizing the measuring tube body with the upper sealing element and the lower sealing element assembled;
step S05: inoculating strains between the inoculation reference line I and the inoculation reference line II;
step S06: culturing edible fungi under proper conditions, wherein the conditions need to consider factors influencing the growth of the strains such as humidity, temperature and the like;
step S07: and recording the growth length by contrasting the scale marks according to the arrival position of the edible fungus hypha in the growth process.
Other embodiments of the disclosure will be apparent to those skilled in the art from consideration of the specification and practice of the disclosure herein. This application is intended to cover any variations, uses, or adaptations of the disclosure following, in general, the principles of the disclosure and including such departures from the present disclosure as come within known or customary practice within the art to which the disclosure pertains. It is intended that the specification and examples be considered as exemplary only, with a true scope and spirit of the disclosure being indicated by the following claims.
It will be understood that the present disclosure is not limited to the precise arrangements described above and shown in the drawings and that various modifications and changes may be made without departing from the scope thereof. The scope of the present disclosure should be limited only by the attached claims.
Claims (10)
1. The device for measuring the growth speed of the hyphae of the edible fungi comprises a measuring tube body and is characterized in that a vent hole group is arranged at the lower part of the measuring tube body, a first inoculation reference line and a second inoculation reference line which are parallel to each other are arranged on the side wall of the upper part of the measuring tube body, and scale marks are carved on the side wall of the measuring tube body;
the device for measuring the growth speed of the hypha of the edible fungus further comprises:
an upper seal member including a sealing plug inserted into the upper opening of the measurement pipe body for sealing the upper portion of the measurement pipe body;
and the lower sealing element comprises a sealing sleeve which is sleeved on the lower part of the measuring pipe body and completely covers the vent hole group.
2. An edible fungus hypha growth rate measuring device according to claim 1, wherein the upper seal further comprises:
the boss is arranged on the side surface of the sealing plug in a surrounding manner;
and the handheld part is arranged on the upper surface of the sealing plug.
3. An edible fungus hypha growth rate measuring device according to claim 2, wherein the sealing plug is a silica gel sealing plug.
4. An edible fungus hypha growth rate measuring device according to claim 1, wherein the lower seal further comprises:
and the sealing ring is arranged at the opening of the sealing sleeve in a surrounding manner, and the sealing ring and the sealing sleeve are integrally formed.
5. The device for measuring the growth rate of the hyphae of the edible fungi according to claim 4, wherein the sealing sleeve is a polyethylene plastic bag or a polypropylene plastic bag.
6. The device for measuring the growth rate of hyphae of edible fungi according to claim 4, wherein the sealing ring is made of rubber.
7. An edible fungus hypha growth rate measuring device according to any one of claims 1-6, wherein the measuring tube body is a PET polyethylene terephthalate tube body or a PC polycarbonate phenol tube body.
8. An edible fungus hypha growth rate measuring device according to claim 7, wherein the vent hole group comprises:
the main vent hole is arranged at the bottommost part of the measuring pipe body;
the plurality of sub-air holes are arranged on the lower part of the measuring pipe body at equal angles by taking the main air hole as the center.
9. An apparatus as claimed in claim 1, wherein the number of the sub-vents is 8-15.
10. A method for measuring the growth rate of an edible fungus hypha according to any one of claims 1-9, wherein the method comprises the following steps:
the method comprises the following steps: filling compost into the measuring pipe body until the capacity of the compost reaches the second inoculation reference line;
step two: covering an upper sealing element, inserting a sealing plug of the upper sealing element into an upper opening of the measuring pipe body, and tightly attaching the sealing plug to the side wall of the opening of the measuring pipe body due to certain elasticity of the sealing plug to realize the sealing of the upper opening of the measuring pipe body;
step three: the sealing sleeve of the lower sealing element is sleeved on the lower part of the measuring pipe body and completely wraps the air holes of the sub-sealing element, and the elasticity of the sealing ring material is utilized to be tightly attached to the side wall of the lower part of the measuring pipe body, so that the sealing of the lower air hole group of the measuring pipe body is realized;
step four: sterilizing the measuring tube body with the upper sealing element and the lower sealing element assembled;
step five: inoculating strains between the inoculation reference line I and the inoculation reference line II;
step six: culturing edible fungi under proper conditions;
step seven: and recording the growth length by contrasting the scale marks according to the arrival position of the edible fungus hypha in the growth process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210067253.5A CN114223461A (en) | 2022-01-20 | 2022-01-20 | Device and method for measuring growth speed of edible fungus hyphae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210067253.5A CN114223461A (en) | 2022-01-20 | 2022-01-20 | Device and method for measuring growth speed of edible fungus hyphae |
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| Publication Number | Publication Date |
|---|---|
| CN114223461A true CN114223461A (en) | 2022-03-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210067253.5A Pending CN114223461A (en) | 2022-01-20 | 2022-01-20 | Device and method for measuring growth speed of edible fungus hyphae |
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| CN (1) | CN114223461A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202335249U (en) * | 2011-08-30 | 2012-07-18 | 宋春领 | Novel special two-mouth cultivation bottle for edible fungi |
| CN205491845U (en) * | 2015-11-18 | 2016-08-24 | 青岛丰科生物科技有限公司 | Edible fungi cultivation bottle |
| CN107223456A (en) * | 2017-07-17 | 2017-10-03 | 山西鑫农联发农业科技园有限公司 | A kind of edible mushroom cultivating device |
| CN207322191U (en) * | 2017-10-31 | 2018-05-08 | 四川农业大学 | A kind of convenient pre-culture spawn bottle of edible mushroom for surveying growth rate and inoculation |
| CN209797968U (en) * | 2019-03-28 | 2019-12-17 | 阜阳职业技术学院 | Edible mushroom hypha growth rate survey box |
| CN213739466U (en) * | 2020-10-15 | 2021-07-20 | 广东省微生物研究所（广东省微生物分析检测中心） | Portable hypha growth rate survey box of portable |
| CN216931172U (en) * | 2022-01-20 | 2022-07-12 | 江西农业大学 | Edible fungus hypha growth speed measuring device |
-
2022
- 2022-01-20 CN CN202210067253.5A patent/CN114223461A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202335249U (en) * | 2011-08-30 | 2012-07-18 | 宋春领 | Novel special two-mouth cultivation bottle for edible fungi |
| CN205491845U (en) * | 2015-11-18 | 2016-08-24 | 青岛丰科生物科技有限公司 | Edible fungi cultivation bottle |
| CN107223456A (en) * | 2017-07-17 | 2017-10-03 | 山西鑫农联发农业科技园有限公司 | A kind of edible mushroom cultivating device |
| CN207322191U (en) * | 2017-10-31 | 2018-05-08 | 四川农业大学 | A kind of convenient pre-culture spawn bottle of edible mushroom for surveying growth rate and inoculation |
| CN209797968U (en) * | 2019-03-28 | 2019-12-17 | 阜阳职业技术学院 | Edible mushroom hypha growth rate survey box |
| CN213739466U (en) * | 2020-10-15 | 2021-07-20 | 广东省微生物研究所（广东省微生物分析检测中心） | Portable hypha growth rate survey box of portable |
| CN216931172U (en) * | 2022-01-20 | 2022-07-12 | 江西农业大学 | Edible fungus hypha growth speed measuring device |
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