CN114191454A - Preparation method of bee therapy product for reducing blood fat - Google Patents
Preparation method of bee therapy product for reducing blood fat Download PDFInfo
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- CN114191454A CN114191454A CN202210075805.7A CN202210075805A CN114191454A CN 114191454 A CN114191454 A CN 114191454A CN 202210075805 A CN202210075805 A CN 202210075805A CN 114191454 A CN114191454 A CN 114191454A
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- propolis
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/64—Insects, e.g. bees, wasps or fleas
- A61K35/644—Beeswax; Propolis; Royal jelly; Honey
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Insects & Arthropods (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Obesity (AREA)
- Hematology (AREA)
- Diabetes (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Zoology (AREA)
- Cardiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Animal Husbandry (AREA)
Abstract
The invention discloses a preparation method of a bee therapy product for reducing blood fat, which comprises the following steps: the preparation method comprises the following steps: freezing propolis at-40 deg.C to-80 deg.C
Pulverizing into fine powder, and sieving with 80-100 mesh sieve; adding pulverized propolis into the powder
The volume percentage concentration of the times is
Adding surfactant into the ethanol solution, extracting with ultrahigh pressure extraction equipment under 30T600Mpa in sodium carbonateAnd heating in the presence of sodium bicarbonate for extraction
Description
Technical Field
The invention relates to the technical field of bee therapy products, in particular to a preparation method of a bee therapy product for reducing blood fat.
Background
Propolis is a viscous substance prepared by collecting vegetable resin from western bees, mixing with beeswax, pollen and gland secretion of bees, and chewing with bees, and has pleasant fragrance. The bee uses propolis to fill the gap of the honeycomb, smoothes the inner wall of the honeycomb, and prevents the rotting of foreign matters and the infection of germs in the honeycomb by the propolis. At present, propolis products at home and abroad are basically extracted by alcohol to obtain effective components. The traditional propolis alcohol extraction method has unacceptable pungent taste, is easy to generate anaphylactic reaction to alcohol allergic persons when in use, increases the allergen, has great limitation when being used as a raw material of cosmetics and oral health care products, and the propolis alcohol extraction liquid is not easy to dissolve in water, has low absorption utilization rate, and has part of water-soluble active ingredients which are not extracted. Other reported extraction methods include propolis extraction with chloroform, ethyl acetate, dichloromethane, glycerol, oil extraction, supercritical extraction, etc., which are costly and do not completely extract the active ingredients. The reported propolis water extraction also adopts cold water extraction or hot reflux, the extraction efficiency is low, and the damage degree of bioactive components is large.
When diabetic dyslipidemia is combined with other atherosclerotic risk factors, the risk of coronary heart disease is greatly increased by the superposition of the risk factors. Such atherosclerotic risk factors are: age, hypertension, smoking and diabetes, postmenopausal women, family history of coronary heart disease, etc., the more risk factors, the greater the probability of onset. Therefore, aiming at diabetes complicated with dyslipidemia, a comprehensive treatment strategy based on syndrome-based medicine is adopted scientifically and reasonably aiming at reducing various risk factors, and the comprehensive treatment strategy comprises measures of effectively reducing weight, reducing insulin resistance, well controlling blood fat, controlling blood pressure, improving lipid metabolism disorder, improving blood circulation and the like.
Disclosure of Invention
The present invention is directed to solving one of the technical problems of the prior art or the related art.
Therefore, the technical scheme adopted by the invention is as follows: a preparation method of a bee therapy product for reducing blood fat comprises the following steps: (1) pre-selecting and grading raw materials, and removing impurities;
(2) placing the pre-selected raw materials into a refrigerated cabinet, freezing at-18 ℃ for 48h, rapidly placing the frozen raw materials into a crusher for crushing, immediately feeding the crushed raw materials into a 20-mesh vibrating screen, and returning large particles to the refrigerated cabinet;
(3) placing the crushed and sieved raw materials into an impregnation kettle, adding 80-95% ethanol, impregnating at normal temperature and continuously stirring, stopping stirring after 6-8h, standing for 2h, discharging supernatant, adding 90% ethanol, repeating the above process for 3 times, and mixing the solutions;
(4) the dipped solution enters a cooling reaction kettle, circular cooling is introduced into a reaction kettle jacket, mechanical stirring is carried out, the temperature is reduced to be below 5 ℃, the cooled solution enters a plate and frame type filter for filtering through a pump when the cooled solution is cold, the temperature of the solution is kept to be below 5 ℃, the filtrate enters a pressure reduction evaporator, and water bath heating is adopted;
(6) recovering ethanol vapor by condenser, injecting into solvent storage tank, recycling, recovering ethanol from filtrate to obtain propolis extract, adding alkaline surface and water, shaking repeatedly to dissolve propolis and alkaline surface, standing for 2-3 hr, and collecting supernatant.
The present invention in a preferred example may be further configured to: in the step (2), the crushing temperature is-5 +/-2 ℃.
By adopting the technical scheme, the propolis is in a semi-plastic state at normal temperature due to the physical properties of the propolis, and is difficult to crush, but is easy to crush at the temperature below-5 ℃.
The present invention in a preferred example may be further configured to: in the step (3), the dipping kettle is a reaction kettle equipped with a reflux condenser, the crude propolis is frozen and crushed, and 95% ethanol is added according to the proportion of 1:4 (g/ml).
The present invention in a preferred example may be further configured to: the temperature is 20-30 ℃ in the dipping process;
by adopting the technical scheme, the beeswax is greatly melted due to overhigh temperature, so that the filtration is difficult; the time is prolonged due to the low solubility caused by the low temperature, and the dosage of the solvent is increased.
The present invention in a preferred example may be further configured to: in the step (4), the heating temperature of the water bath is 30-45 ℃.
By adopting the technical scheme, the temperature is kept below 50 ℃ at this stage, so that volatilization and evaporation and thermal denaturation of active ingredients can be reduced. .
A bee therapy product for reducing blood lipid comprises propolis extract prepared by the above preparation method.
The beneficial effects obtained by the invention are as follows:
1. according to the invention, the water bath reflux ethanol extraction method is adopted to extract the effective components in the propolis, the water bath is utilized to recover and circulate the ethanol, the method is suitable for large-scale obtaining of the propolis ethanol extract, and the temperature in the water bath stage is kept below 50 ℃, so that the volatilization evaporation and the thermal denaturation of the effective components can be reduced, the retention of the effective active components in the extract is improved, and the actual efficacy of the peak therapy product is enhanced.
2. In the invention, the peak therapy product is rich in flavonoid compounds, quercetin, ruta graveolens and other functional factors, and the mechanism of the peak therapy product is probably related to the inhibition of glucosidase activity, lipid peroxidation resistance, improvement of vascular endothelial function and improvement of blood microcirculation, so that the peak therapy product can effectively reduce the contents of cholesterol, triglyceride and low-density lipoprotein in blood, prevent cardiovascular and cerebrovascular diseases and has the health-care function of regulating blood fat.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be noted that the embodiments of the present invention and features of the embodiments may be combined with each other without conflict.
It is to be understood that this description is made only by way of example and not as a limitation on the scope of the invention.
The following describes a method for preparing a hypolipidemic bee therapy product according to some embodiments of the present invention.
The invention provides a preparation method of a bee therapy product for reducing blood fat, which comprises the following steps: (1) pre-selecting and grading raw materials, and removing impurities;
(2) placing the pre-selected raw materials into a refrigerated cabinet, freezing at-18 ℃ for 48h, rapidly placing the frozen raw materials into a crusher for crushing, immediately feeding the crushed raw materials into a 20-mesh vibrating screen, and returning large particles to the refrigerated cabinet;
(3) placing the crushed and sieved raw materials into an impregnation kettle, wherein the impregnation kettle is a reaction kettle equipped with a reflux condenser, freezing and crushing crude propolis, adding 95% ethanol according to the proportion of 1:4(g/ml), impregnating at normal temperature and continuously stirring, stopping stirring after 6-8h, standing for 2h, discharging supernatant, adding 90% ethanol, repeating the above process for 3 times, and combining the solution;
(4) the dipped solution enters a cooling reaction kettle, circular cooling is introduced into a reaction kettle jacket, mechanical stirring is carried out, the temperature is reduced to be below 5 ℃, the cooled solution enters a plate and frame type filter for filtering through a pump when the cooled solution is cold, the temperature of the solution is kept to be below 5 ℃, the filtrate enters a pressure reduction evaporator, and water bath heating is adopted;
(6) recovering ethanol vapor by condenser, injecting into solvent storage tank, recycling, recovering ethanol from filtrate to obtain propolis extract, adding alkaline surface and water, shaking repeatedly to dissolve propolis and alkaline surface, standing for 2-3 hr, and collecting supernatant.
In this example, the crushing temperature in step (2) was-5. + -.2 ℃ and it was difficult to crush propolis since it was in a semi-plastic state at normal temperature due to its physical properties, but crushing was easy at a temperature below-5 ℃.
In this example, the temperature during impregnation is 20-30 ℃;
specifically, the beeswax is greatly melted due to the over-high temperature, which causes difficulty in filtration; the time is prolonged due to the low solubility caused by the low temperature, and the dosage of the solvent is increased.
In this example, the heating temperature of the water bath in the step (4) was 30 to 45 ℃.
In particular, the temperature should be kept below 50 ℃ at this stage, so that evaporation and thermal denaturation of the active ingredients can be reduced.
A bee therapy product for reducing blood lipid comprises propolis extract prepared by the above preparation method.
Animal experiments:
1. establishing a hyperlipemia rat model: male SD rats of about 300g were fed with high-fat diet and were fed freely and drunk water, rats fed with normal diet were additionally used as normal controls, and two weeks later, blood was drawn from the orbit to determine the blood lipid cholesterol (TC) content and serum SOD activity, to judge the success of the model.
2. Administration: randomly grouping rats with abnormal blood lipid metabolism, wherein each group comprises 10 rats, providing bee therapy products for reducing blood lipid, feeding mice with 5g of each meal, feeding once every 8h, and taking blood from orbit after 4 weeks; blood lipid lowering and antioxidant effects;
3. and (3) testing: the content of serum cholesterol (TC) is measured by a full-automatic biochemical analyzer; measuring oxidation index with superoxide dismutase (SOD);
the test results show that, compared with the hyperlipemia animal model group, the embodiment can reduce the Total Cholesterol (TC) in the serum of the experimental rat and can increase the SOD activity in the serum. Quercetin and rutin are most representative, quercetin has effects of dilating coronary blood vessel, reducing blood lipid and blood pressure, etc., while rutin has effects of softening capillary vessel, enhancing vascular permeability, reducing cholesterol, etc. The inventors have found that. Meanwhile, the above technical idea of the present invention can be also discussed in the reverse direction, for example, the disadvantage of the health care composition can be illustrated when the content of each component is out of the scope of the claims of the present invention, so as to highlight the content of each component as the advantage of the technical feature embodying the inventive idea. In addition, after the technical solutions of the dependent claims are written, the invention concept can also be explained by referring to the above method, and the technical problems, the technical solutions and the technical effects are organically combined to highlight the intelligent contribution of the invention.
In the description herein, the description of the terms "one embodiment," "some embodiments," "specific embodiments," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims.
Claims (6)
1. A preparation method of a bee therapy product for reducing blood fat is characterized by comprising the following steps: (1) pre-selecting and grading raw materials, and removing impurities;
(2) placing the pre-selected raw materials into a refrigerated cabinet, freezing at-18 ℃ for 48h, rapidly placing the frozen raw materials into a crusher for crushing, immediately feeding the crushed raw materials into a 20-mesh vibrating screen, and returning large particles to the refrigerated cabinet;
(3) placing the crushed and sieved raw materials into an impregnation kettle, adding 80-95% ethanol, impregnating at normal temperature and continuously stirring, stopping stirring after 6-8h, standing for 2h, discharging supernatant, adding 90% ethanol, repeating the above process for 3 times, and mixing the solutions;
(4) the dipped solution enters a cooling reaction kettle, circular cooling is introduced into a reaction kettle jacket, mechanical stirring is carried out, the temperature is reduced to be below 5 ℃, the cooled solution enters a plate and frame type filter for filtering through a pump when the cooled solution is cold, the temperature of the solution is kept to be below 5 ℃, the filtrate enters a pressure reduction evaporator, and water bath heating is adopted;
(6) recovering ethanol vapor by condenser, injecting into solvent storage tank, recycling, recovering ethanol from filtrate to obtain propolis extract, adding alkaline surface and water, shaking repeatedly to dissolve propolis and alkaline surface, standing for 2-3 hr, and collecting supernatant.
2. The method for preparing bee therapy product for reducing blood lipid according to claim 1, wherein the crushing temperature in step (2) is-5 ± 2 ℃.
3. The method for preparing bee therapy products for reducing blood lipid according to claim 1, wherein the dipping kettle in step (3) is a reaction kettle equipped with a reflux condenser, the crude propolis is frozen and pulverized, and 95% ethanol is added according to a ratio of 1:4 (g/ml).
4. The method for preparing bee therapy product for reducing blood lipid according to claim 1, wherein the temperature during the dipping process is 20-30 ℃.
5. The method for preparing bee therapy product for reducing blood lipid according to claim 1, wherein the heating temperature of the water bath in the step (4) is 30-45 ℃.
6. A bee therapy product for reducing blood lipid, characterized by comprising the propolis extract obtained by the production method of any one of claims 1 to 5.
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