CN114191342A - Skin care lotion for promoting skin metabolism and preparation method thereof - Google Patents
Skin care lotion for promoting skin metabolism and preparation method thereof Download PDFInfo
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- CN114191342A CN114191342A CN202111391047.1A CN202111391047A CN114191342A CN 114191342 A CN114191342 A CN 114191342A CN 202111391047 A CN202111391047 A CN 202111391047A CN 114191342 A CN114191342 A CN 114191342A
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- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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Abstract
The invention is suitable for the technical field of skin lotion, has provided A skin lotion and its preparation method to promote skin metabolism, make the skin care product made of other substances according to certain proportion to three kinds of purpose-made liquids one, two purpose-made liquids and three purpose-made liquids have good compatibility with human body, it is easy to be absorbed by the human body, have good promoting effects to the cell growth of the skin, can promote the wound healing of the skin, accelerate the cell metabolism, strengthen the cell activity of the skin, wherein one purpose-made liquid can promote the expression of LAMIN-A, LAP-2, RANBP-17 protein, in order to resume the healthy form of the cell, and then reach the improvement of the skin, skin keratinocyte; the second special liquid adopts materials emitting far infrared rays, can improve the microcirculation of the skin by being smeared on the skin, promotes the blood circulation and improves the immunity of the skin; the third special liquid contains cell factors with cell life nature, which become the most suitable for human skin cells, and has the functions of promoting skin wound healing and repairing damaged skin surface.
Description
Technical Field
The invention belongs to the technical field of skin care lotion, and particularly relates to skin care lotion for promoting skin metabolism and a preparation method thereof.
Background
The skin is the largest organ covering the surface of the human body, and is easily damaged by the stimulation of various tension factors such as oxidation, dryness, ultraviolet rays and the like due to direct contact with the external environment. Particularly, in winter, the whole metabolism of a human body becomes slower, the metabolism of skin also becomes slower, the cutin of the epidermis of the skin cannot be metabolized normally, and the cutin is accumulated on the surface of the skin, so that cells at the bottom layer of the epidermis are pressed, the skin cannot absorb nutrition, metabolic toxins are accumulated in the skin, various skin problems are caused, the resistance of the skin is reduced, and the normal life of people is inconvenient.
Disclosure of Invention
The invention provides a skin care lotion for promoting skin metabolism and a preparation method thereof, aiming at solving the problems in the background technology.
The invention is realized in such a way that the skin care lotion for promoting skin metabolism is prepared by mixing three special liquids, wherein the special liquid comprises phytol, a wild soybean seed extract, 1,3 propanediol and glycerol;
the second special liquid comprises: sorbitol fatty acid ester, lanolin, 70% maltitol, and glycerol; liquid paraffin, sodium cholate, 1, 3-butanediol, methyl p-hydroxybenzoate, water and perfume;
the special liquid III is skin repair essence;
preferably, the special liquid I comprises the following components in part ratio: phytol: 0.1-3 parts; extract of soybean seeds: 0.1-2 parts; 1,3 propylene glycol: 3 parts of a mixture; glycerol: 5 parts of the raw materials.
Preferably, the second special preparation comprises the following components in parts by weight: 2.5 of sorbitol fatty acid ester; lanolin 5; 70% maltitol 10; 15 of glycerol; 20 parts of liquid paraffin; 5, sodium cholate; 1, 3-butanediol 5; 0.5 of methylparaben; water 35; a proper amount of spice;
preferably, the second special material also comprises silver oxide, aluminum oxide, yttrium oxide, aluminum oxide, silicon oxide, magnesium oxide, zirconium oxide and titanium oxide.
A preparation method of skin care lotion for promoting skin metabolism comprises: the method comprises the following steps:
the method comprises the following steps: preparing a special solution I;
step two: preparing a special liquid II;
step three: preparing a special solution III;
step four: mixing the special liquid I, the special liquid II and the special liquid III.
Preferably, the step one comprises the following steps:
s1: screening raw materials: preparing basic ingredients according to the weight ratio of a special liquid composition formula: phytol: 0.1-3 parts; extract of soybean seeds: 0.1-2 parts; 1,3 propylene glycol: 3 parts of a mixture; glycerol: 5 parts of a mixture;
s2: stirring for the first time: mixing alcohol: 0.1-3 parts and glycerol: dispersing 5 parts of the mixture in an ultrasonic oscillator, fully and uniformly mixing, and then pouring the mixture into a homogenizer for homogenizing for 3min to obtain a mixture for later use;
s3: stirring for the second time: extracting the wild soybean seeds: 0.1-2 parts and 1,3 propylene glycol: adding 3 parts of the mixture obtained in the step S1, fully and uniformly stirring the mixture in an ultrasonic oscillator, and cooling the mixture to 40 ℃ to obtain the first special liquid.
Preferably, the step two comprises the following steps:
s4, heating 2.5 parts of sorbitol fatty acid ester, 5 parts of lanolin, 15 parts of glycerol and 20 parts of liquid paraffin to 90 ℃ by a water bath method to melt, heating 5 parts of 1, 3-butanediol and 10 parts of 70% maltitol to 50 ℃ to uniformly mix, adding sodium cholate and uniformly stirring.
S5, uniformly stirring 1 part of silver oxide, 1 part of aluminum oxide, 1 part of yttrium oxide, 1 part of aluminum oxide, 1 part of silicon oxide, 1 part of magnesium oxide, 1 part of zirconium oxide and 1 part of titanium oxide with 35 parts of water, pouring into the prepared maltitol and butanediol, uniformly mixing again, and finally cooling the materials to 50 ℃ and uniformly mixing to prepare the special liquid II.
Preferably, the method comprises the following steps in the third step:
preparation of S6 culture solution:
preparing a serum-free culture solution of the mesenchymal stem cells, wherein the components of the serum-free culture solution contain amino acid, vitamin, sugar, trace elements, unsaturated fatty acid, antioxidant and cell growth factor; the method comprises the following specific steps:
mesenchymal stem cell culture solution: DMEM-LG + F12+ BSA + bFGF + TGF-beta + PDGF
S7 cell culture:
subjecting the mesenchymal stem cells in logarithmic phase to 1x104From ml to 1X106Inoculating the culture solution with a density of one ml in the prepared culture solution, and culturing at 37 ℃ for 3-72 hours; the mesenchymal stem cells are mammalian mesenchymal stem cells and comprise bone marrow mesenchymal stem cells, umbilical cord mesenchymal stem cells and placenta mesenchymal stem cells;
s8 cell activation:
activating the mesenchymal stem cells by adopting a method of co-culturing another type of cells and original cells in a culture solution or a method of adding cell factors into the cell culture solution, so that the mesenchymal stem cells secrete substances which are beneficial to skin repair and vitality enhancement;
s9 cell removal, supernatant harvest:
removing all cells in the culture solution, and collecting the supernatant after cell culture to obtain the skin repair essence rich in the cell activity factors.
S10 degradation and preparation:
adding substance capable of decomposing protein (preferably trypsin) into the skin repair essence prepared in the previous step, degrading macromolecular protein into short peptide, and removing substance with molecular weight over 3500 Dalton to obtain skin repair essence rich in cell active factor and suitable for absorption by human skin, i.e. specially-made liquid III
Preferably, S11, adding the special liquid I, the special liquid II and the special liquid III according to the adding proportion of 20 percent, 30 percent and 20 percent by weight, adding a proper amount of purified water, stirring and mixing to form colloid.
Compared with the prior art, the invention has the beneficial effects that: the special preparation liquid I can promote the expression of LAMIN-A, LAP-2 and RANBP-17 proteins, increase and improve the transport capacity of nuclear pore complexes of cell nuclei, increase the transport amount of the proteins and mRNA in the nuclear pore complexes, further improve the health of the cell nuclei, restore the health of cells, further improve skin and skin keratinocytes, and effectively improve skin aging by matching with the extract of wild soybean seeds, thereby promoting the generation of collagen and elastin
The second special liquid adopts sorbitol fatty acid ester, lanolin, 70% maltitol, glycerin, liquid paraffin, sodium cholate, 1, 3-butanediol, methyl p-hydroxybenzoate and materials which emit far infrared rays and are niobium oxide, tantalum oxide, yttrium oxide, aluminum oxide, silicon oxide, zirconium oxide and magnesium oxide, because the microcirculation of the human body can be improved, the microcirculation of the skin can be improved by being smeared on the skin, the blood circulation is promoted, the immunity of the skin is improved, the sodium cholate is taken as a material with active ingredients and has compatibility with the skin and physiology of the human body, the two main materials can both contribute to the growth of skin biological enzyme, and the dual functions of facial health care and metabolism promotion are achieved;
TIMP-1 and MCP-1 in the third special liquid have the effect of promoting skin wound healing, and can be used for treating skin wound and diabetic foot; bFGF and EGF have functions of repairing skin tissue and promoting skin cell proliferation, and can be used for treating burn and scald skin. The collection of cytokines from mammalian stem cell cultures, which are cell-vital, is the most suitable source of nutrients for the growth of human skin cells and other organs. Has effects in promoting skin wound healing, repairing damaged skin surface, and promoting skin regeneration.
The beauty skin care product prepared by mixing the three special liquids has good compatibility with a human body, is easy to be absorbed by the human body, has good promotion effect on the growth of skin cells, can promote the healing of skin wound surfaces, accelerate cell metabolism and enhance the activity of the skin cells.
Drawings
FIG. 1 is a schematic flow chart of the steps of the preparation method of the skin care lotion for promoting skin metabolism according to the present invention;
FIG. 2 is a partially enlarged view of the first schematic diagram in FIG. 1;
FIG. 3 is a partially enlarged view of the second embodiment of FIG. 1;
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Referring to fig. 1-3, the present invention provides a technical solution:
a skin care lotion for promoting skin metabolism comprises three special liquids, wherein the special liquid comprises phytol, semen glycines extract, 1, 3-propylene glycol, and glycerol; wherein the first special liquid comprises the following components in percentage by weight: phytol: 0.1-3 parts; extract of soybean seeds: 0.1-2 parts; 1,3 propylene glycol: 3 parts of a mixture; glycerol: 5 parts of the raw materials.
The second special liquid comprises: sorbitol fatty acid ester, lanolin, 70% maltitol, and glycerol; liquid paraffin, sodium cholate, 1, 3-butanediol, methyl p-hydroxybenzoate, water and perfume; the second special preparation comprises the following components in parts by weight: 2.5 of sorbitol fatty acid ester; lanolin 5; 70% maltitol 10; 15 of glycerol; 20 parts of liquid paraffin; 5, sodium cholate; 1, 3-butanediol 5; 0.5 of methylparaben; water 35; a proper amount of spice; the second special material also comprises silver oxide, aluminum oxide, yttrium oxide, aluminum oxide, silicon oxide, magnesium oxide, zirconium oxide and titanium oxide.
The special liquid III is skin repair essence;
a preparation method of skin care lotion for promoting skin metabolism comprises: the method comprises the following steps:
the method comprises the following steps: preparing a special solution I; the first step comprises the following steps:
s1: screening raw materials: preparing basic ingredients according to the weight ratio of a special liquid composition formula: phytol: 0.1-3 parts; extract of soybean seeds: 0.1-2 parts; 1,3 propylene glycol: 3 parts of a mixture; glycerol: 5 parts of a mixture;
s2: stirring for the first time: mixing alcohol: 0.1-3 parts and glycerol: dispersing 5 parts of the mixture in an ultrasonic oscillator, fully and uniformly mixing, and then pouring the mixture into a homogenizer for homogenizing for 3min to obtain a mixture for later use;
s3: stirring for the second time: extracting the wild soybean seeds: 0.1-2 parts and 1,3 propylene glycol: adding 3 parts of the mixture obtained in the step S1, fully and uniformly stirring the mixture in an ultrasonic oscillator, and cooling the mixture to 40 ℃ to obtain the first special liquid.
In the first step of the present embodiment:the phytol used in the first special liquid adopts mosscelltecno.1 of miloler biochemical, switzerland, Lysofix of Kemin of wild soybean seed extract; the temperature reduction in step S3 is natural cooling; the model of the homogenizer is RX-200l in step S2.
Step two: preparing a special liquid II; the second step comprises the following steps:
s4, heating 2.5 parts of sorbitol fatty acid ester, 5 parts of lanolin, 15 parts of glycerol and 20 parts of liquid paraffin to 90 ℃ by a water bath method to melt, heating 5 parts of 1, 3-butanediol and 10 parts of 70% maltitol to 50 ℃ to uniformly mix, adding sodium cholate and uniformly stirring.
S5, uniformly stirring 1 part of silver oxide, 1 part of aluminum oxide, 1 part of yttrium oxide, 1 part of aluminum oxide, 1 part of silicon oxide, 1 part of magnesium oxide, 1 part of zirconium oxide and 1 part of titanium oxide with 35 parts of water, pouring into the prepared maltitol and butanediol, uniformly mixing again, and finally cooling the materials to 50 ℃ and uniformly mixing to prepare the special liquid II.
Step three: preparing a special solution III; the third step comprises the following steps:
preparation of S6 culture solution:
preparing a serum-free culture solution of the mesenchymal stem cells, wherein the components of the serum-free culture solution contain amino acid, vitamin, sugar, trace elements, unsaturated fatty acid, antioxidant and cell growth factor; the method comprises the following specific steps:
mesenchymal stem cell culture solution: DMEM-LG + F12+ BSA + bFGF + TGF-beta + PDGF
S7 cell culture:
inoculating the mesenchymal stem cells in logarithmic phase into the culture solution prepared in the last step at the density of 1x10^4/ml to 1x10^6/ml, and culturing for 3-72 hours at 37 ℃; the mesenchymal stem cells are mammalian mesenchymal stem cells and comprise bone marrow mesenchymal stem cells, umbilical cord mesenchymal stem cells and placenta mesenchymal stem cells;
s8 cell activation:
activating the mesenchymal stem cells by adopting a method of co-culturing another type of cells and original cells in a culture solution or a method of adding cell factors into the cell culture solution, so that the mesenchymal stem cells secrete substances which are beneficial to skin repair and vitality enhancement;
s9 cell removal, supernatant harvest:
removing all cells in the culture solution, and collecting the supernatant after cell culture to obtain the skin repair essence rich in the cell activity factors.
S10 degradation and preparation:
adding a substance capable of decomposing protein (preferably trypsin) into the skin repair essence prepared in the last step, degrading macromolecular protein into short peptide, and removing substances with molecular weight of more than 3500 daltons to obtain the skin repair essence which is rich in cell active factors and is suitable for being absorbed by human skin, namely specially-made liquid III.
In step three of the present embodiment: the method for removing the cells in the culture solution in step S9 is centrifugation and/or filtration.
The specific cell activation method described in step S8 of the above method is as follows:
mesenchymal stem cell activation method 1 (co-culture method):
A. preparing a neural stem cell culture solution: StemPro neural Stem cell serum-free culture Medium (StemProNSCSFM, available from Life technologies, Cat. A10509-01) + GlutaMax supplement (available from Life technologies, Cat. 35050-
B. Inoculating the neural stem cells into the culture solution prepared in the last step at the density of 1x10^4/ml to 5x10^5/ml, and culturing for 24-72 hours until the logarithmic phase;
C. and (3) harvesting the neural stem cells (such as a centrifugal method), adding the neural stem cells into the mesenchymal stem cell culture in the step (2) in a quantity ratio of 2: 1-1: 2, and co-culturing for 12-24 hours.
Mesenchymal stem cell activation method 2 (culture concentrate method with addition of secretion of neural stem cells):
A. preparing a neural stem cell culture solution: StemPro neural Stem cell serum-free culture Medium (StemProNSCSFM, available from Life technologies, Cat. A10509-01) + GlutaMax supplement (available from Life technologies, Cat. 35050-
B. Inoculating the neural stem cells into the culture solution prepared in the last step at the density of 1x10^5/ml to 5x10^5/ml, and culturing for 24-48 hours;
C. removing the neural stem cells (by centrifugation or filtration), and concentrating the rest culture solution (containing neural stem cell secretion) to 1/5-1/20 (the concentration ratio is hooked with the subsequent addition ratio; if the concentration ratio is 1/5, the next addition ratio is 2-20%, if the concentration ratio is 1/20, the next addition ratio is 0.5-5%, and if the concentration ratio is 1/10, the next addition ratio is 1-10%);
D. adding the concentrated solution into the mesenchymal stem cell culture solution in the step (2) in a volume ratio of 0.5-20%, and continuing to culture for 6-24 hours.
The components of the neural stem cell culture solution are as follows:
StemPro neural Stem cell serum-free culture medium (StemProNSCSFM) is a well-known culture medium kit in the art, and can be purchased from Lifetechnologies, Inc., article No. A10509-01, and the kit consists of KNOCKOUTDMEM/F12 (article No. 12660-012), recombinant human bFGF (article No. PHG0024), recombinant human EGF (article No. PHG0314), and StemProurelsupply (article No. A10508-01).
KNOCKOUTDMEM/F12 is a low osmolarity medium supplied by Life technologies, Inc. without L-glutamine or HEPES buffer, cat No. 12660-.
bFGF (basic fibroblast growth factor) is a cytokine known in the art, and has a strong mitogenic proliferative activity on a variety of cells such as fibroblasts, vascular endothelial cells, neurons and glial cells.
EGF (epidermal growth factor) is a cytokine known in the art that stimulates epithelial cells and the proliferation of various cells.
StemProneuralesupplent is a serum-free nutritional supplement provided by Life technologies, Inc., Cat No. A10508-01, and can be used for growth and expansion of human and murine neural stem cells and neural progenitor cells.
GlutaMax supplement is available from Life technologies, Inc. under Cat 35050-.
Step four: s11, adding the special liquid I, the special liquid II and the special liquid III according to the adding proportion of 20 percent, 30 percent and 20 percent by weight, adding a proper amount of purified water, stirring and mixing to form colloid.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (8)
1. A skin care lotion for promoting skin metabolism, which is characterized in that: comprises three special liquids, wherein the special liquid comprises phytol, wild soybean seed extract, 1, 3-propanediol and glycerol;
the second special liquid comprises: sorbitol fatty acid ester, lanolin, 70% maltitol, and glycerol; liquid paraffin, sodium cholate, 1, 3-butanediol, methyl p-hydroxybenzoate, water and perfume;
the third special liquid is skin repair essence.
2. The skin lotion according to claim 1 for promoting skin metabolism, which comprises: wherein the first special liquid comprises the following components in percentage by weight: phytol: 0.1-3 parts; extract of soybean seeds: 0.1-2 parts; 1,3 propylene glycol: 3 parts of a mixture; glycerol: 5 parts of the raw materials.
3. The skin lotion according to claim 1 for promoting skin metabolism, which comprises: the second special preparation comprises the following components in parts by weight: 2.5 of sorbitol fatty acid ester; lanolin 5; 70% maltitol 10; 15 of glycerol; 20 parts of liquid paraffin; 5, sodium cholate; 1, 3-butanediol 5; 0.5 of methylparaben; water 35; a proper amount of spice;
the second special material also comprises silver oxide, aluminum oxide, yttrium oxide, aluminum oxide, silicon oxide, magnesium oxide, zirconium oxide and titanium oxide.
4. A method for preparing a skin lotion for promoting skin metabolism comprising preparing a skin lotion for promoting skin metabolism according to any one of claims 1 to 3, wherein: the method comprises the following steps:
the method comprises the following steps: preparing a special solution I;
step two: preparing a special liquid II;
step three: preparing a special solution III;
step four: mixing the special liquid I, the special liquid II and the special liquid III.
5. The method for preparing a skin lotion for promoting skin metabolism according to claim 4, wherein: the first step comprises the following steps:
s1: screening raw materials: preparing basic ingredients according to the weight ratio of a special liquid composition formula: phytol: 0.1-3 parts; extract of soybean seeds: 0.1-2 parts; 1,3 propylene glycol: 3 parts of a mixture; glycerol: 5 parts of a mixture;
s2: stirring for the first time: mixing alcohol: 0.1-3 parts and glycerol: dispersing 5 parts of the mixture in an ultrasonic oscillator, fully and uniformly mixing, and then pouring the mixture into a homogenizer for homogenizing for 3min to obtain a mixture for later use;
s3: stirring for the second time: extracting the wild soybean seeds: 0.1-2 parts and 1,3 propylene glycol: adding 3 parts of the mixture obtained in the step S1, fully and uniformly stirring the mixture in an ultrasonic oscillator, and cooling the mixture to 40 ℃ to obtain the first special liquid.
6. The method for preparing a skin lotion for promoting skin metabolism according to claim 4, wherein: the second step comprises the following steps:
s4, heating 2.5 parts of sorbitol fatty acid ester, 5 parts of lanolin, 15 parts of glycerol and 20 parts of liquid paraffin to 90 ℃ by a water bath method to melt, heating 5 parts of 1, 3-butanediol and 10 parts of 70% maltitol to 50 ℃ to uniformly mix, adding sodium cholate and uniformly stirring;
s5, uniformly stirring 1 part of silver oxide, 1 part of aluminum oxide, 1 part of yttrium oxide, 1 part of aluminum oxide, 1 part of silicon oxide, 1 part of magnesium oxide, 1 part of zirconium oxide and 1 part of titanium oxide with 35 parts of water, pouring into the prepared maltitol and butanediol, uniformly mixing again, and finally cooling the materials to 50 ℃ and uniformly mixing to prepare the special liquid II.
7. The method for preparing a skin lotion for promoting skin metabolism according to claim 4, wherein: the third step comprises the following steps:
preparation of S6 culture solution:
preparing a serum-free culture solution of the mesenchymal stem cells, wherein the components of the serum-free culture solution contain amino acid, vitamin, sugar, trace elements, unsaturated fatty acid, antioxidant and cell growth factor; the method comprises the following specific steps:
mesenchymal stem cell culture solution: DMEM-LG + F12+ BSA + bFGF + TGF- β + PDGF;
s7 cell culture:
subjecting the mesenchymal stem cells in logarithmic phase to 1x104From ml to 1X106Inoculating the culture solution with a density of one ml in the prepared culture solution, and culturing at 37 ℃ for 3-72 hours; the mesenchymal stem cells are mammalian mesenchymal stem cells and comprise bone marrow mesenchymal stem cells, umbilical cord mesenchymal stem cells and placenta mesenchymal stem cells;
s8 cell activation:
activating the mesenchymal stem cells by adopting a method of co-culturing another type of cells and original cells in a culture solution or a method of adding cell factors into the cell culture solution, so that the mesenchymal stem cells secrete substances which are beneficial to skin repair and vitality enhancement;
s9 cell removal, supernatant harvest:
removing all cells in the culture solution, and collecting the supernatant after cell culture to obtain the skin repair essence rich in the cell activity factors.
S10 degradation and preparation:
adding a substance capable of decomposing protein (preferably trypsin) into the skin repair essence prepared in the last step, degrading macromolecular protein into short peptide, and removing substances with molecular weight of more than 3500 daltons to obtain the skin repair essence which is rich in cell active factors and is suitable for being absorbed by human skin, namely specially-made liquid III.
8. The method for preparing a skin lotion for promoting skin metabolism according to claim 4, wherein: in the fourth step, S11, adding the special liquid I, the special liquid II and the special liquid III according to the adding proportion of 20 percent, 30 percent and 20 percent by weight, and adding a proper amount of purified water to stir and mix the mixture to form colloid.
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| CN1509708A (en) * | 2002-12-23 | 2004-07-07 | 冯小英 | Skin caring liquor prescription for promoting metabolism and its preparation |
| CN103550117A (en) * | 2013-10-18 | 2014-02-05 | 上海润生生物技术有限公司 | Beauty and skin care product capable of promoting skin repairation and regeneration as well as preparation method and application thereof |
| CN110215421A (en) * | 2019-05-21 | 2019-09-10 | 广州市拉凯尔干细胞研究所 | A kind of skin care item preparation method promoting healthy gene expression in horn cell core |
| JP2021001166A (en) * | 2019-06-19 | 2021-01-07 | ハンス ファーマ カンパニー リミテッドＨａｎｓ Ｐｈａｒｍａ Ｃｏ．，Ｌｔｄ． | Composition for improving skin comprising neural stem cell culture solution |
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2021
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1509708A (en) * | 2002-12-23 | 2004-07-07 | 冯小英 | Skin caring liquor prescription for promoting metabolism and its preparation |
| CN103550117A (en) * | 2013-10-18 | 2014-02-05 | 上海润生生物技术有限公司 | Beauty and skin care product capable of promoting skin repairation and regeneration as well as preparation method and application thereof |
| CN110215421A (en) * | 2019-05-21 | 2019-09-10 | 广州市拉凯尔干细胞研究所 | A kind of skin care item preparation method promoting healthy gene expression in horn cell core |
| JP2021001166A (en) * | 2019-06-19 | 2021-01-07 | ハンス ファーマ カンパニー リミテッドＨａｎｓ Ｐｈａｒｍａ Ｃｏ．，Ｌｔｄ． | Composition for improving skin comprising neural stem cell culture solution |
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