CN114190459A

CN114190459A - Auxiliary blood sugar-reducing tea bag and preparation method thereof

Info

Publication number: CN114190459A
Application number: CN202111575060.2A
Authority: CN
Inventors: 孟庆雄; 余杨宏
Original assignee: Kunming University of Science and Technology
Current assignee: Kunming University of Science and Technology
Priority date: 2021-12-21
Filing date: 2021-12-21
Publication date: 2022-03-18

Abstract

The invention belongs to the technical field of health-care food and functional beverage, and particularly relates to an auxiliary blood sugar-reducing tea bag and a preparation method thereof. The invention relates to a tea bag for reducing blood sugar, which is prepared from mulberry leaf tea, gymnema sylvestre, barley grass, guava, honeysuckle flower and wolfberry fruit according to a certain weight proportion. The tea bag has scientific formula, simple preparation process, convenient drinking, safety and reliability, does not show obvious toxic effect on various observation indexes of male and female rats through safety toxicology tests, and can effectively improve various sugar metabolism indexes (including fasting blood glucose, serum insulin level, C peptide, glucagon and the like) of a type 2diabetes model mouse (db/db mouse) through effect verification tests, 1000mL of drinking water is brewed in each tea bag, 200mL of drinking water is drunk before each meal, and the tea bag has ideal auxiliary treatment effect on type 2diabetes patients.

Description

Auxiliary blood sugar-reducing tea bag and preparation method thereof

Technical Field

The invention belongs to the technical field of health-care food and functional beverage, and particularly relates to an auxiliary blood sugar-reducing tea bag and a preparation method thereof.

Background

Diabetes Mellitus (DM) is a non-infectious disease of chronic metabolic disorders caused by defects in pancreatic islet function. With the development of economy, the living standard of people is gradually improved, and DM is gradually the third most non-infectious disease in the world after cardiovascular diseases and tumors. The international diabetes union (IDF) website (http:// www.diabetesatlas.org /) published, and the number of DM patients in 2017 worldwide adults is about 4.25 million, wherein the number of DM patients in China is as high as 1.14 million. Chinese guidelines for the prevention and treatment of type 2diabetes (2017 edition) also clearly indicate that the prevalence rate of DM in adults in China is as high as 10.9%. Among the numerous DM patients, Type 2diabetes mellitus (T2 DM) accounts for approximately 90% to 95% of the total DM. Research shows that patients suffering from T2DM are very likely to induce diseases such as coronary heart disease, cardiomyopathy, hypertension and hyperlipidemia, and have great harm to human bodies.

Since the etiology and pathogenesis of diabetes are not completely understood, there is currently a lack of effective etiological treatment. At present, the diabetes is clinically treated by mostly adopting insulin treatment and oral hypoglycemic drugs, however, diabetes belongs to a lifelong disease, long-term medication is needed, the injection administration method of insulin often brings inconvenience to patients, and clinically commonly used oral hypoglycemic drugs such as insulin secretagogues, biguanides, alpha-glycosidase inhibitors, thiazolidinedione derivatives, DPP-4 enzyme inhibitors and the like belong to chemical drugs or biological drugs, and adverse reactions such as gastrointestinal reactions, nausea, constipation, diarrhea, headache, dizziness, liver and kidney injuries and the like are caused after long-term administration, so that the tolerance and compliance of patients are reduced. In view of the above problems, it is very urgent to develop a safe, effective and blood sugar-lowering health food or functional beverage suitable for long-term administration in the current medical and health product market.

Disclosure of Invention

In order to overcome the defects of the existing diabetes treatment medicines, the invention provides a solution. China has abundant natural medicine resources and a long-term tea drinking culture, and the application history of natural medicines (such as traditional Chinese medicines) in traditional medicine is long-running, and nowadays, under the large background of modernization of traditional Chinese medicines, natural medicines are more and more favored by people due to the advantages of definite curative effect, high safety, low toxicity, low price and the like. Based on the reasons, the inventor develops a new tea bag with auxiliary hypoglycemic effect through long-term screening and repeated trial production, the tea bag is prepared by mulberry leaf tea, gymnema sylvestre, barley grass, guava, honeysuckle and medlar according to a certain weight proportion formula, the tea bag has scientific formula, the tea bag has the advantages of simple preparation process, convenience in drinking, safety and reliability, and has no obvious toxic effect on various observation indexes of male and female rats through safety toxicology tests, and effect verification tests prove that the tea bag can effectively improve various sugar metabolism indexes (including fasting blood glucose FBG, insulin level, C peptide, glucagon and the like) of a type 2diabetes model mouse (db/db mouse), 1000mL of drinking water is brewed in each tea bag, 200mL of 100 + one meal is drunk before each meal, and the tea bag has an obvious auxiliary treatment effect on type 2diabetes patients.

Specifically, the present invention is realized by the following technical means.

In a first aspect, the invention provides an auxiliary blood sugar reducing tea bag, which is prepared from the following raw materials in parts by weight:

30-40 parts of mulberry leaf tea, 20-25 parts of gymnema sylvestre, 20-25 parts of barley grass, 10-15 parts of guava, 5-10 parts of honeysuckle and 3-5 parts of wolfberry fruit.

Preferably, the auxiliary blood sugar reducing tea bag is prepared from the following raw materials in parts by weight:

33 parts of mulberry leaf tea, 22 parts of gymnema sylvestre, 21 parts of barley grass, 13 parts of guava, 7 parts of honeysuckle and 4 parts of wolfberry fruit.

The auxiliary blood sugar reducing tea bag is prepared from six raw materials, and the specific functions of the raw materials are as follows:

mulberry leaf tea: the folium Mori water extract can inhibit activity of maltase and lactase, and reduce absorption of above two kinds of sugar by organism. The folium Mori can promote insulin release and inhibit activity of alpha-glycosidase. The mulberry leaves can not only protect pancreas and promote normal secretion of insulin, but also improve hypertension and reduce cholesterol and triglyceride.

Guava: the guava contains high content of aromatic secondary metabolites (polyphenol compounds), which can improve the utilization rate of peripheral tissues to glucose and promote the combination of insulin and receptors, and in addition, the guava contains a large amount of chromium elements, so that chromium supplementation is helpful for improving the glucose tolerance of diabetics and people with abnormal glucose tolerance, reducing blood sugar and blood fat and enhancing the sensitivity of insulin.

A gymnema sylvestre: gymnema sylvestre has effects of lowering blood sugar, reducing blood lipid, resisting dental caries, inhibiting bacteria and regulating immunity. Gymnemic acid is a triterpenoid saponin substance separated from gymnema sylvestre, is a main bioactive component in gymnema sylvestre, and has various biological activities such as inhibition of small intestine absorption of oleic acid, inhibition of activity of glyceraldehyde triphosphate dehydrogenase, inhibition of protein synthesis, inhibition of activity of alpha-glucosidase, and inhibition of activity of sodium-dependent glucose transporter 1.

Barley seedling: as a nutritional crop, the barley seedling is often used for preparing health care products with the functions of resisting cancers, resisting oxidation, expelling toxin and delaying senility. In the research, the barley grass component is added into the blood sugar reducing tea bag according to a certain proportion, so that the effect of the tea bag on improving various sugar metabolism indexes of a type 2diabetes model mouse can be obviously improved, and the barley grass component and other components in the formula are judged to generate an active synergistic blood sugar reducing effect.

Honeysuckle flower: the honeysuckle is good for clearing away heat and toxic materials in the heart and stomach, contains quercetin, kaempferol, luteolin and other components, and the quercetin can improve INS-1 cell activity under high glucose stimulation, lower the relative expression quantity of BAX and PDX-1mRNA, inhibit INS-1 cell oxidative stress, reduce apoptosis and promote insulin secretion of beta cells; kaempferol can increase calcium flow in mitochondria under sugar stimulation, thereby promoting insulin secretion induced by glucose, and can promote insulin metabolism and secretion by regulating calcium absorption of mitochondria, and kaempferol can also prevent islet beta cells from oxidative damage or improve islet beta cell activity damaged by chronic hyperglycemia; luteolin can improve insulin beta cell damage induced by thapsigargin or uric acid, and improve glycolipid metabolism.

Wolfberry fruit: the medlar can increase the level of serum insulin, repair the damaged islet cells and enhance the sugar tolerance.

Furthermore, the invention also relates to application of the auxiliary blood sugar reducing tea bag in preparation of a medicine for treating type 2 diabetes.

Further, the invention also provides a preparation method of the auxiliary blood sugar reducing tea bag, which comprises the following steps:

(1) cleaning fresh mulberry leaves, deactivating enzyme with steam for 5min, controlling the temperature at 110-120 ℃, kneading for 5-10min after the deactivation of enzyme is completed, drying in a constant-temperature blast dryer at 40 ℃ until the water content is lower than 10%, then putting into a swing type traditional Chinese medicine crusher for crushing, and sieving with a 80-mesh sieve after crushing to obtain the mulberry leaf tea;

(2) cleaning Gymnema sylvestre, barley seedling, guava, honeysuckle and wolfberry fruit, respectively putting into a 40 ℃ constant-temperature blast drier or a 40 ℃ constant-temperature oven for drying until the water content is lower than 10%, respectively putting into a swing type traditional Chinese medicine crusher for crushing, and sieving by a 80-mesh sieve after crushing to obtain the raw tea powder of Gymnema sylvestre, barley seedling, guava, honeysuckle and wolfberry fruit;

(3) weighing the raw tea powder in corresponding parts by weight according to the formula, uniformly mixing, weighing 20-30g, and filling into a tea bag to obtain the auxiliary blood sugar reducing tea bag.

In a second aspect, the invention provides a functional solid beverage package for reducing blood sugar, which comprises 20-30g of the auxiliary blood sugar-reducing tea bag.

In a third aspect, the invention also provides a blood sugar reducing composition, which comprises the following raw materials in parts by weight:

In conclusion, the auxiliary blood sugar reducing tea bag is prepared from six raw materials, namely mulberry leaf tea, gymnema sylvestre, barley grass, guava, honeysuckle and wolfberry fruit, is mainly prepared from mulberry leaf tea, gymnema sylvestre and barley grass, is prepared from the guava, the honeysuckle and the wolfberry fruit, and has ideal auxiliary treatment effect on type 2diabetes under the synergistic effect. The tea bag can be drunk before a long-term meal, has the function of obviously reducing fasting and postprandial blood sugar and glucagon, and further realizes the purpose of improving the glycolipid metabolism of an organism. In addition, the tea bag has the advantages of convenience in taking, high safety, low price and the like, and is very suitable for the current market demand.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, it is obvious that the drawings in the following description are only specific embodiments mentioned for the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the following drawings without inventive efforts.

FIG. 1 is a graph showing a comparison of fasting blood glucose levels between a blank control group and each example group before and after tea drinking.

Detailed Description

The technical solutions of the present invention are described below clearly and completely by specific examples, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Other advantages and features of the present invention will be readily apparent to those skilled in the art from the disclosure herein. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention.

Before the present embodiments are further described, it is to be understood that the scope of the invention is not limited to the particular embodiments described below; it is also to be understood that the terminology used in the examples is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention.

When numerical ranges are given in the examples, it is understood that both endpoints of each of the numerical ranges and any value therebetween can be selected unless the invention otherwise indicated. Unless otherwise defined, all technical terms used in the present invention have the same meaning as commonly understood by one of ordinary skill in the art. In addition to the specific methods, devices, and materials used in the examples, any methods, devices, and materials similar or equivalent to those described in the examples herein can be used in the practice of the invention in addition to the specific methods, devices, and materials used in the examples herein, in keeping with the knowledge of one skilled in the art and the description of the present invention.

In the invention, all parts and percentages are by weight unless otherwise specified, and all equipment and raw materials are commercially available or commonly used in the industry unless otherwise specified. The methods in the following examples are conventional in the art unless otherwise specified.

Example 1

An auxiliary blood sugar reducing tea bag is prepared from the following raw materials in parts by weight:

30 parts of mulberry leaf tea, 25 parts of gymnema sylvestre, 20 parts of barley grass, 10 parts of guava, 10 parts of honeysuckle and 5 parts of wolfberry fruit.

The preparation method comprises the following steps:

(1) cleaning fresh mulberry leaves, deactivating enzyme with steam for 5min at 120 deg.C, kneading for 10min, drying in 40 deg.C constant temperature blast drier until water content is less than 10%, pulverizing in a swing type traditional Chinese medicine pulverizer, and sieving with 80 mesh sieve to obtain mulberry leaf tea;

(2) cleaning Gymnema Yunnanense Tsiang, barley grass, guava, honeysuckle and medlar, respectively putting into a constant temperature oven at 40 ℃ for drying until the water content is lower than 10%, respectively putting into a swing type traditional Chinese medicine crusher for crushing, and sieving by a sieve of 80 meshes after crushing to obtain raw tea powder of Gymnema Yunnanense Tsiang, barley grass, guava, honeysuckle and medlar;

(3) weighing the raw tea powder according to the formula in corresponding parts by weight, uniformly mixing, weighing 25g, and filling into a tea bag to obtain the auxiliary blood sugar reducing tea bag, wherein 1 tea bag is taken each time and is added with boiling water for brewing for 12min, each tea bag is brewed with 1000mL of drinking water, and the volume of drinking water before each meal is 100-200 mL.

Example 2

36 parts of mulberry leaf tea, 20 parts of gymnema sylvestre, 23 parts of barley grass, 12 parts of guava, 6 parts of honeysuckle and 3 parts of wolfberry fruit.

The preparation method is the same as example 1.

Example 3

The preparation method is the same as example 1.

Example 4

Evaluation test for blood sugar reducing effect of auxiliary blood sugar reducing tea bag

(1) Grouping

Blank control group: purified water for brewing tea.

Example 1 group: 30 parts of mulberry leaf tea, 25 parts of gymnema sylvestre, 20 parts of barley grass, 10 parts of guava, 10 parts of honeysuckle and 5 parts of wolfberry fruit.

Example 2 group: 36 parts of mulberry leaf tea, 20 parts of gymnema sylvestre, 23 parts of barley grass, 12 parts of guava, 6 parts of honeysuckle and 3 parts of wolfberry fruit.

Example 3 group: 33 parts of mulberry leaf tea, 22 parts of gymnema sylvestre, 21 parts of barley grass, 13 parts of guava, 7 parts of honeysuckle and 4 parts of wolfberry fruit.

(2) Experimental animals and feeds

Experimental animals: db/db mice, male, four-week-old, supplied by Jiangsu Jiejiekang Biotech, having a weight of 20.82 + -1.57 g when purchased, and fed with SPF. The qualification number of the experimental animal: 202100532, license number: scxk (threo) 2018-.

Basic feed: 20g of flour, 10g of rice flour, 20g of corn, 25g of bran, 20g of bean material, 2g of bone meal, 2g of fish meal, 0.9g of salt and 0.1g of vitamin.

(3) Feeding and tea soup feeding mode

After the experimental animals are purchased, the basic feed is normally and adaptively fed for one week (SPF feeding, namely feeding the animals in animal rooms with the temperature of 23 +/-2 ℃, the humidity of 40-50 percent, artificial illumination and light and dark for 12 hours respectively). Then randomly divided into: blank control, example 1, example 2 and example 3. The blank control group does not carry out any treatment, and the blank control group drinks the purified water used for making tea for eating at a specific time and supplies the purified water without limitation; the auxiliary blood sugar reducing tea soup brewed according to the formula and the preparation method is drunk by the groups of the embodiments, and the auxiliary blood sugar reducing tea soup is eaten at a specific time and is eaten without limitation; continuously feeding for four weeks.

(4) Observation indicator and measurement method

During the test period, the body weight was weighed 1 time per week and appetite, behavior, feces, hair and animal death were observed. Fasting blood glucose was measured with a glucometer after 10h of fasting every week. Taking blood from heart of mouse by cutting spine after four weeks, placing whole blood in refrigerator at 4 deg.C for 30min, cooling to 4 deg.C in advance with low temperature centrifuge, centrifuging (3600r/min, 15min) to separate serum, carefully sucking upper layer serum, and storing in refrigerator at-80 deg.C. The contents of insulin, C-peptide and glucagon in the serum of mice were determined using a murine ELISA kit.

(5) Test results and comparative analysis

During the test period, db/db mice grow normally in body weight, move normally, have good mental status and do not die. After 28 days of administration, fasting plasma glucose levels and serum level analysis for each group of mice are shown in FIG. 1 and Table 1, respectively.

A comparison of fasting plasma glucose levels between the blank control group and the example group before and after drinking tea is shown in FIG. 1. As can be seen from fig. 1, there was no significant difference in fasting blood glucose between the example group before drinking tea and the blank control group, and the example 3 group had the best effect of lowering fasting blood glucose with increasing drinking time, and there was a significant difference from the blank control group (. about.p <0.01 was a significant difference, and. about.p <0.05 was a significant difference).

The effect on serum levels in db/db mice after 4 weeks of tea consumption is shown in Table 1. It can be seen that serum insulin and C-peptide were significantly elevated in the db/db mice of the example group compared to the blank control group, wherein the group of example 3 was very significantly different from the blank control group; glucagon was significantly reduced in the example group compared to the blank control group. The above results confirmed that the tea bag of the present invention has a significant hypoglycemic effect, and the group of example 3 was most excellent in reducing fasting plasma glucose and glucagon in mice, and increasing insulin and C-peptide to improve glycolipid metabolism.

TABLE 1 Effect on serum levels in db/db mice after 4 weeks of tea consumption

(significant difference is P <0.01, significant difference is P <0.05)

And (4) test conclusion: (1) in the example 1, the fasting blood sugar, the insulin and the C peptide have significant difference (P <0.05) with the blank control group, and have the function of assisting to reduce the blood sugar; in the example 2, the fasting blood sugar, the insulin, the C peptide and the glucagon in the group have significant difference (P <0.05) with the blank control group, and have the effect of significantly assisting in reducing the blood sugar; the results of the group 3, in which fasting plasma glucose, insulin, C-peptide and glucagon are significantly (P <0.05) or very significantly different (P <0.01) from the blank control group, showed more significant hypoglycemic effects, and all of the group 3 had hypoglycemic and glycolipid metabolism improving effects, with the group 3 being the best.

The tea soup of 3 groups of examples is taken, and safety toxicology tests show that the tea soup does not produce obvious toxic effect on various observation indexes of female and male rats, so that the tea soup is safe to take and does not cause toxic reaction.

The tea bag (or the pharmaceutical composition) can be taken alone to assist in reducing blood sugar, and can also be used in combination with blood sugar reducing drugs, such as metformin, acarbose, gliquidone tablets and the like, so as to enhance the blood sugar reducing effect of the blood sugar reducing drugs.

While the preferred embodiments and examples of the present invention have been described in detail, the present invention is not limited to the embodiments and examples, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.

Claims

1. The auxiliary blood sugar-reducing tea bag is characterized by being prepared from the following raw materials in parts by weight:

2. The auxiliary hypoglycemic tea bag of claim 1, wherein the weight parts of the raw materials are:

3. Use of the auxiliary blood glucose-lowering tea bag according to claim 1 or 2 in the preparation of a medicament for treating type 2 diabetes.

4. The preparation method of the auxiliary hypoglycemic tea bag according to claim 1 or 2, comprising the steps of:

5. A functional solid beverage package for reducing blood sugar, which is characterized in that 20-30g of the auxiliary blood sugar reducing tea bag as claimed in claim 1 or 2 is contained in the functional solid beverage package for reducing blood sugar.

6. The blood sugar reducing composition is characterized by comprising the following raw materials in parts by weight: