CN114167780A - Surface water source microorganism concentration monitoring system based on ATP technology - Google Patents
Surface water source microorganism concentration monitoring system based on ATP technology Download PDFInfo
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- CN114167780A CN114167780A CN202111468298.5A CN202111468298A CN114167780A CN 114167780 A CN114167780 A CN 114167780A CN 202111468298 A CN202111468298 A CN 202111468298A CN 114167780 A CN114167780 A CN 114167780A
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- G05B—CONTROL OR REGULATING SYSTEMS IN GENERAL; FUNCTIONAL ELEMENTS OF SUCH SYSTEMS; MONITORING OR TESTING ARRANGEMENTS FOR SUCH SYSTEMS OR ELEMENTS
- G05B19/00—Programme-control systems
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- G05B19/04—Programme control other than numerical control, i.e. in sequence controllers or logic controllers
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Abstract
ATP technology-based surface water source microorganism concentration monitoring system, the monitoring system includes: the shell is an oval shell; the controller is arranged in the shell and is electrically connected with a communication assembly, a clock assembly, an input assembly, an execution assembly, a sampling assembly, a lifting assembly, a power supply assembly and a reaction assembly; the sampling assembly is used for collecting a water body sample from a surface water source to be monitored; the lifting assembly is used for adjusting different positions of the shell in the surface water source so that the shell can be suspended at different depths in the surface water source, and sampling is convenient; the power supply assembly is used for providing stable and durable power supply voltage for the controller; the reaction assembly is used for injecting a reaction reagent into a water body sample; the execution assembly is used for monitoring a corresponding luminous value of the water body sample after reaction, converting the luminous value into a corresponding electric signal and inputting the electric signal into the controller so as to obtain a microorganism concentration parameter in the water body sample.
Description
The technical field is as follows:
the invention relates to a system for monitoring the concentration of microorganisms in a surface water source based on an ATP technology.
Background art:
the surface water source is fresh water quantity which can be updated year by year in the surface water, is an important component of water resources and consists of various water bodies distributed on the earth surface, such as rivers, lakes, marshes, glaciers and the like; from the water supply perspective, surface water sources refer to fresh water occurring in rivers, lakes and glaciers; from the perspective of shipping and farming, surface water sources mainly refer to water occurring in river channels and water areas; from the viewpoint of energy utilization, the surface water source mainly refers to river runoff with a certain fall.
The dynamic water amount of the surface water source is river runoff and glacier runoff, and the static water amount is expressed by the water storage amount of various water bodies; the worldwide water reserves of surface water sources are 24254 billions of cubic meters, which only account for 1.75 percent of the total water reserves in the world; however, the surface water body is continuously replenished by atmospheric rainfall, and 43.5 billion cubic meters of river runoff and 2.3 billion cubic meters of glacier runoff flow into the ocean every year through flow generation and confluence, account for 94.7 percent of the total amount of the ocean, and play a very important role in global water circulation; in addition, the runoff of rivers generated in the inflow region is 1.0 billion cubic meters every year, the runoff is imported into inland lakes to be consumed in evaporation, and the form of a surface water source is closely related to the climate.
Generally, microorganisms with relative quantity and concentration can degrade organic matters, release inorganic nutrients and circulate inorganic elements to achieve the aim of purifying water; therefore, in the surface water source, the concentration parameter of the microorganisms in the water body is an important monitoring parameter, the quantity of the concentration parameter can intuitively reflect the quality of the water body of the surface water source, and according to the national sanitation standard, the concentration parameter of the microorganisms in the water body is required to be in a relatively stable mathematical interval and set, so that the actual use requirement can be met.
In order to ensure the quality of the surface water source and reduce the pollution of the surface water source, the surface water source needs to be regularly monitored for microorganism concentration, and the quality of the corresponding surface water source is evaluated and judged according to the microorganism concentration obtained by monitoring; the total number of bacterial colonies in drinking water is less than or equal to 100CFU/mL as specified in GB5749-2006 sanitary Standard for Drinking Water; the total number of bacterial colonies in the small centralized water supply body and the distributed water supply body is less than or equal to 500CFU/mL, and the like; the existing traditional monitoring mode usually calculates the concentration of microorganisms by monitoring the biological oxygen consumption in unit time, the acquisition difficulty of oxygen consumption data is high, the data obtained by monitoring is not visual and clear enough, and the later evaluation calculation is easy to generate larger errors; meanwhile, the whole steps of the existing oxygen consumption monitoring mode are complicated, the automation degree is low, the actual workload of workers in the operation process is high, and the popularization difficulty is high.
The invention content is as follows:
the embodiment of the invention provides an ATP technology-based surface water source microorganism concentration monitoring system, which has reasonable structure and method design, is matched with various types of electric elements and equipment components based on the integrated control action of an ATP fluorescence detection technology and a single chip microcomputer controller, and converts light signals into corresponding electric signals by utilizing the bioluminescence principle after combining the ATP and the ATP luciferase and the direct proportion relation between the luminous intensity and the ATP content, thereby realizing the purpose of monitoring the microorganism concentration in real time, reducing the acquisition difficulty of monitoring data, enabling the monitoring data to be more intuitive and accurate and avoiding the generation of monitoring errors; meanwhile, the overall monitoring step and the actual working process are simplified, the popularization and the promotion are easy to carry out under different use scenes, and the problems in the prior art are solved.
The technical scheme adopted by the invention for solving the technical problems is as follows:
ATP technology-based surface water source microorganism concentration monitoring system, the monitoring system includes:
the device comprises a shell, a water tank, a water inlet pipe, a water outlet pipe, a water inlet pipe, a water outlet pipe and a water outlet pipe, wherein the shell is an oval shell and is used for being placed in a surface water source to be monitored to monitor the concentration parameters of microorganisms;
the controller is arranged in the shell and is electrically connected with a communication assembly, a clock assembly, an input assembly, an execution assembly, a sampling assembly, a lifting assembly, a power supply assembly and a reaction assembly; the communication assembly is used for establishing wireless network communication between the controller and the upper computer so as to remotely transmit monitoring parameters; the clock component is used for setting monitoring frequency; the input assembly is used for inputting a monitoring instruction to the controller to enable the controller to enter a working state; the sampling assembly is used for collecting a water body sample from a surface water source to be monitored; the lifting assembly is used for adjusting different positions of the shell in the surface water source so that the shell can be suspended at different depths in the surface water source, and sampling is convenient; the power supply assembly is used for providing stable and durable power supply voltage for the controller; the reaction assembly is used for injecting a reaction reagent into a water body sample; the execution assembly is used for monitoring a corresponding luminous value of the water body sample after reaction, converting the luminous value into a corresponding electric signal and inputting the electric signal into the controller so as to obtain a microorganism concentration parameter in the water body sample.
The model of controller is STM32F103C8T6, is equipped with 64 pins on the controller, the controller links to each other with the power supply subassembly through a pin, the controller links to each other with the input module through No. four pins, the controller links to each other with the executive component through No. fifteen pins, the controller links to each other with the communication subassembly through No. twenty pin, No. twenty-one pin and No. twenty-three pin, the controller links to each other with the reaction subassembly through No. thirty-three pin and No. thirty-four pin, the controller links to each other with lifting unit and sampling unit through No. thirty-eight pin, the controller links to each other with the clock subassembly through No. forty-five pin.
The power supply assembly comprises a solar cell panel, a voltage stabilizer and a lithium battery which are electrically connected, the solar cell panel is arranged at the top of the shell, the lithium battery is arranged at the bottom in the shell, and the voltage stabilizer is used for continuously and stably charging the lithium battery; the model of stabiliser is LM317, is equipped with three pin on the stabiliser, the stabiliser links to each other with solar cell panel through a pin, is equipped with fourth electric capacity and fifth resistance between a pin and No. two pins of stabiliser, is equipped with fifth electric capacity and sixth resistance between No. two pins and No. three pins of stabiliser, No. three pins of stabiliser link to each other with the lithium cell, No. one pin of lithium cell and controller links to each other.
The input assembly comprises a command input device, the type of the command input device is TLP290, four pins are arranged on the command input device, a first pin of the command input device is a monitoring command input pin, a ninth resistor, a tenth resistor and a fourth capacitor are connected in parallel between a first pin and a second pin of the command input device, a fifth capacitor and an eighth resistor are connected in parallel between a third pin and a fourth pin of the command input device, and a third pin of the command input device is connected with a fourth pin of the controller.
The execution assembly comprises an AD converter, a temperature sensor and a photoelectric sensor are connected to the AD converter, the temperature sensor is used for monitoring a temperature value of the water body sample after reaction, and the photoelectric sensor is used for monitoring a luminous value of the water body sample after reaction so as to obtain the microbial concentration of the water body sample; the AD converter is characterized in that the model of the AD converter is AD8551, 8 pins are arranged on the AD converter, the AD converter is connected with a No. fifteen pin of the controller through a No. six pin, and the AD converter is connected with the photoelectric sensor and the temperature sensor through a No. three pin; the model of the photoelectric sensor is OH-1021, and the model of the temperature sensor is SHT 20.
The communication component comprises a GPRS communicator, the model of the GPRS communicator is SIM800C, 42 pins are arranged on the GPRS communicator, a first pin of the GPRS communicator is connected with a twenty-first pin of the controller, a second pin of the GPRS communicator is connected with a twenty-first pin of the controller, a sixth pin of the GPRS communicator is connected with a twenty-second pin of the controller, and a sixth resistor and a seventh resistor which are connected in parallel are arranged between the second pin and the sixth pin of the GPRS communicator; the GPRS communicator is connected with an SIM card through a pin fifteen, a pin sixteen, a pin seventeen and a pin eighteen, a fourth capacitor, a fifth capacitor and a sixth capacitor are respectively arranged on the pin fifteen, the pin sixteen and the pin seventeen of the GPRS communicator, a seventh capacitor is connected to the pin eighteen of the GPRS communicator, and an antenna is connected to the pin thirty-two of the GPRS communicator.
The reaction assembly comprises a trigger and a plunger pump which are electrically connected, and the plunger pump is used for injecting a reaction reagent into a water body sample; the type of the trigger is FDS9945, the pin II of the trigger is connected with the pin thirty-eight of the controller, the pin III of the trigger is connected with the pin thirty-seven of the controller, and the pin eight and the pin five of the trigger are connected with a plunger pump.
The sampling assembly comprises a driver, a peristaltic pump and a linear push rod which are electrically connected, the peristaltic pump is used for sampling a water body sample and injecting the water body sample into the fluorescent reaction tube, and the linear push rod is used for adjusting the depth position of the peristaltic pump so as to sample at different depths of a surface water source; the linear push rod is vertically and downwards arranged at the bottom of the shell, and a piston rod of the linear push rod is provided with a peristaltic pump; the lifting assembly comprises a driver and a water pump which are electrically connected, and the water pump is arranged in the water injection bins at two ends of the bottom of the shell so as to adjust the mass of water in the water injection bins to change the depth of the shell; the driver is of the type ULN2003, 16 pins are arranged on the driver, the driver is connected with thirty-eight pins of the controller through a pin I, a first relay is connected to a pin sixteen of the driver, a first resistor and a first diode which are connected in parallel are arranged on the first relay, and an equipment interface is arranged on the first relay and used for electrically connecting the peristaltic pump, the linear push rod and the water pump.
The clock assembly comprises a timer, the type of the timer is DS1302, 8 pins are arranged on the timer, a fourth resistor and a fourth capacitor which are connected in parallel are arranged between a sixth pin and a seventh pin of the timer, and the seventh pin of the timer is connected with a forty-five pin of the controller.
The two ends of the shell are respectively provided with a limiting rubber pad, and the limiting rubber pads are used for keeping the shell in a vertical position, so that the shell is not deviated and is prevented from colliding with the external environment; a proximity switch is arranged in each limit rubber pad, the model of the proximity switch is FCS004, and the proximity switch is connected with a forty-six pin of the controller; and a stabilizing bracket and a positioning anchor are also arranged at the bottom of the shell.
By adopting the structure, the power supply assembly provides stable and lasting power supply voltage for the controller, so that the whole monitoring system can stably work for a long time; inputting a monitoring control command to the controller through the input assembly to enable the controller to enter a working state; wireless network communication is established between the upper computer and the controller through the communication assembly so as to remotely transmit monitoring parameters; monitoring a corresponding luminous value of the reacted water body sample through an execution assembly to obtain a microorganism concentration parameter in the water body sample; injecting a reaction reagent into the water body sample through the reaction assembly, and collecting the water body sample from a surface water source to be monitored through the sampling assembly; adjust the different positions of casing in the earth's surface water source through lifting unit to make the different degree of depth of casing in the earth's surface water source suspend, conveniently carry out sampling operation, have simple and convenient practicality, accurate high efficiency, advantage that degree of automation is high.
Description of the drawings:
FIG. 1 is a schematic structural diagram of the present invention.
FIG. 2 is a schematic diagram of an execution module according to the present invention.
FIG. 3 is a schematic structural diagram of a sampling assembly according to the present invention.
Fig. 4 is a schematic structural diagram of the lifting assembly of the present invention.
Fig. 5 is a schematic structural diagram of the power supply assembly of the present invention.
FIG. 6 is a schematic structural view of a reaction module according to the present invention.
Fig. 7 is an electrical schematic of the controller of the present invention.
Fig. 8 is an electrical schematic diagram of the voltage regulator of the present invention.
FIG. 9 is an electrical schematic of the command input unit of the present invention.
Fig. 10 is an electrical schematic diagram of the AD converter of the present invention.
Fig. 11 is an electrical schematic diagram of the photosensor of the present invention.
Fig. 12 is an electrical schematic of the temperature sensor of the present invention.
Fig. 13 is an electrical schematic of the proximity switch of the present invention.
Fig. 14 is an electrical schematic diagram of the GPRS communicator of the present invention.
Fig. 15 is an electrical schematic of the trigger of the present invention.
Fig. 16 is a schematic structural view of the plunger pump of the present invention.
Fig. 17 is an electrical schematic of the actuator of the present invention.
Fig. 18 is an electrical schematic of the timepiece of the invention.
In the figure, the solar water heater comprises a shell 1, a shell 2, a solar panel 3, a water injection bin 4, a limiting rubber mat 5, a proximity switch 6, a linear push rod 7, a peristaltic pump 8, a stabilizing support 9 and a positioning anchor.
The specific implementation mode is as follows:
in order to clearly explain the technical features of the present invention, the following detailed description of the present invention is provided with reference to the accompanying drawings.
As shown in fig. 1-18, an ATP technology based surface water source microorganism concentration monitoring system, the monitoring system comprising:
the device comprises a shell 1, wherein the shell 1 is an oval shell, and the shell 1 is used for being placed in a surface water source to be monitored to monitor the concentration parameters of microorganisms;
the controller is arranged in the shell 1, and is electrically connected with a communication assembly, a clock assembly, an input assembly, an execution assembly, a sampling assembly, a lifting assembly, a power supply assembly and a reaction assembly; the communication assembly is used for establishing wireless network communication between the controller and the upper computer so as to remotely transmit monitoring parameters; the clock component is used for setting monitoring frequency; the input assembly is used for inputting a monitoring instruction to the controller to enable the controller to enter a working state; the sampling assembly is used for collecting a water body sample from a surface water source to be monitored; the lifting assembly is used for adjusting different positions of the shell 1 in the surface water source so that the shell 1 can be suspended at different depths in the surface water source, and sampling is convenient; the power supply assembly is used for providing stable and durable power supply voltage for the controller; the reaction assembly is used for injecting a reaction reagent into a water body sample; the execution assembly is used for monitoring a corresponding luminous value of the water body sample after reaction, converting the luminous value into a corresponding electric signal and inputting the electric signal into the controller so as to obtain a microorganism concentration parameter in the water body sample.
The model of controller is STM32F103C8T6, is equipped with 64 pins on the controller, the controller links to each other with the power supply subassembly through a pin, the controller links to each other with the input module through No. four pins, the controller links to each other with the executive component through No. fifteen pins, the controller links to each other with the communication subassembly through No. twenty pin, No. twenty-one pin and No. twenty-three pin, the controller links to each other with the reaction subassembly through No. thirty-three pin and No. thirty-four pin, the controller links to each other with lifting unit and sampling unit through No. thirty-eight pin, the controller links to each other with the clock subassembly through No. forty-five pin.
The power supply assembly comprises a solar cell panel 2, a voltage stabilizer and a lithium battery which are electrically connected, wherein the solar cell panel 2 is arranged at the top of the shell 1, the lithium battery is arranged at the bottom in the shell 1, and the voltage stabilizer is used for continuously and stably charging the lithium battery; the model of stabiliser is LM317, is equipped with three pin on the stabiliser, the stabiliser links to each other with solar cell panel through a pin, is equipped with fourth electric capacity and fifth resistance between a pin and No. two pins of stabiliser, is equipped with fifth electric capacity and sixth resistance between No. two pins and No. three pins of stabiliser, No. three pins of stabiliser link to each other with the lithium cell, No. one pin of lithium cell and controller links to each other.
The input assembly comprises a command input device, the type of the command input device is TLP290, four pins are arranged on the command input device, a first pin of the command input device is a monitoring command input pin, a ninth resistor, a tenth resistor and a fourth capacitor are connected in parallel between a first pin and a second pin of the command input device, a fifth capacitor and an eighth resistor are connected in parallel between a third pin and a fourth pin of the command input device, and a third pin of the command input device is connected with a fourth pin of the controller.
The execution assembly comprises an AD converter, a temperature sensor and a photoelectric sensor are connected to the AD converter, the temperature sensor is used for monitoring a temperature value of the water body sample after reaction, and the photoelectric sensor is used for monitoring a luminous value of the water body sample after reaction so as to obtain the microbial concentration of the water body sample; the AD converter is characterized in that the model of the AD converter is AD8551, 8 pins are arranged on the AD converter, the AD converter is connected with a No. fifteen pin of the controller through a No. six pin, and the AD converter is connected with the photoelectric sensor and the temperature sensor through a No. three pin; the model of the photoelectric sensor is OH-1021, and the model of the temperature sensor is SHT 20.
The communication component comprises a GPRS communicator, the model of the GPRS communicator is SIM800C, 42 pins are arranged on the GPRS communicator, a first pin of the GPRS communicator is connected with a twenty-first pin of the controller, a second pin of the GPRS communicator is connected with a twenty-first pin of the controller, a sixth pin of the GPRS communicator is connected with a twenty-second pin of the controller, and a sixth resistor and a seventh resistor which are connected in parallel are arranged between the second pin and the sixth pin of the GPRS communicator; the GPRS communicator is connected with an SIM card through a pin fifteen, a pin sixteen, a pin seventeen and a pin eighteen, a fourth capacitor, a fifth capacitor and a sixth capacitor are respectively arranged on the pin fifteen, the pin sixteen and the pin seventeen of the GPRS communicator, a seventh capacitor is connected to the pin eighteen of the GPRS communicator, and an antenna is connected to the pin thirty-two of the GPRS communicator.
The reaction assembly comprises a trigger and a plunger pump which are electrically connected, and the plunger pump is used for injecting a reaction reagent into a water body sample; the type of the trigger is FDS9945, the pin II of the trigger is connected with the pin thirty-eight of the controller, the pin III of the trigger is connected with the pin thirty-seven of the controller, and the pin eight and the pin five of the trigger are connected with a plunger pump.
The sampling assembly comprises a driver, a peristaltic pump 7 and a linear push rod 6 which are electrically connected, wherein the peristaltic pump 7 is used for sampling a water body sample and injecting the water body sample into the fluorescent reaction tube, and the linear push rod is used for adjusting the depth position of the peristaltic pump 7 so as to sample at different depths of a surface water source; the linear push rod 6 is vertically and downwards arranged at the bottom of the shell 1, and a piston rod of the linear push rod 6 is provided with a peristaltic pump 7; the lifting assembly comprises a driver and a water pump which are electrically connected, and the water pump is arranged in the water injection bins 3 at two ends of the bottom of the shell 1 so as to adjust the mass of water in the water injection bins 3 to change the depth of the shell 1; the driver is of the type ULN2003, 16 pins are arranged on the driver, the driver is connected with thirty-eight pins of the controller through a pin I, a first relay is connected to a pin sixteen of the driver, a first resistor and a first diode which are connected in parallel are arranged on the first relay, and an equipment interface is arranged on the first relay and used for electrically connecting the peristaltic pump 7, the linear push rod 6 and the water pump.
The clock assembly comprises a timer, the type of the timer is DS1302, 8 pins are arranged on the timer, a fourth resistor and a fourth capacitor which are connected in parallel are arranged between a sixth pin and a seventh pin of the timer, and the seventh pin of the timer is connected with a forty-five pin of the controller.
The two ends of the shell 1 are respectively provided with a limiting rubber pad 4, and the limiting rubber pads 4 are used for keeping the shell 1 in a vertical position, so that the shell 1 is not deviated and is prevented from colliding with an external environment; a proximity switch 5 is arranged in each limit rubber mat 4 respectively, the type of the proximity switch 5 is FCS004, and the proximity switch 5 is connected with a forty-six number pin of the controller; a stabilizing bracket 8 and a positioning anchor 9 are also mounted at the bottom of the housing 1.
The ATP technology-based surface water source microorganism concentration monitoring system in the embodiment of the invention has the working principle that: based on the integrated control effect of an ATP fluorescence detection technology and a single chip microcomputer controller, the optical signal is converted into a corresponding electric signal by matching with various types of electric elements and equipment components and utilizing the bioluminescence principle after combining the ATP and the ATP luciferase and the direct proportion relation between the luminous intensity and the ATP content, so that the aim of monitoring the concentration of the microorganisms in real time is fulfilled, the acquisition difficulty of monitoring data is reduced, the monitoring data is more visual and accurate, and the generation of monitoring errors is avoided; meanwhile, the overall monitoring step and the actual working process are simplified, and the method is easy to popularize and popularize under different use scenes, so that the concentration parameters of microorganisms in different water areas can be accurately obtained.
In the integral scheme, a controller STM32F103C8T6 is taken as a core component, 64 pins are arranged on the controller, the controller is connected with a power supply component through a pin I, the controller is connected with an input component through a pin IV, the controller is connected with an execution component through a pin V, the controller is connected with a communication component through a pin twenty, a pin twenty-one and a pin twenty-three, the controller is connected with a reaction component through a pin thirty-thirteen and a pin thirty-four, the controller is connected with a lifting component and a sampling component through a pin thirty-eight, the controller is connected with a clock component through a pin forty-fifteen, so that an integral hardware circuit is formed, different functional components play corresponding functions by depending on the integral hardware circuit, and the integral hardware circuit is matched with an oval shell structure to quickly and accurately monitor microbe concentration parameters corresponding to a surface water source, and then the data parameters are timely and remotely transmitted to the staff.
In the actual use process, firstly, 220V direct current is used for fully charging the lithium battery, then the device is placed in surface water source places such as lakes, reservoirs, rivers and the like in a manual throwing mode, the device is fixed in a certain area of a water body under the action of a stable bracket 8 and a positioning anchor 9 at the bottom of the shell 1, the position of the shell 1 is kept relatively stable, and deviation is avoided; and meanwhile, the limiting rubber pads 4 and the proximity switches 5 at two ends of the shell 1 are matched to prevent the shell 1 from colliding with the external environment, so that the normal operation of the whole monitoring system is ensured.
After the equipment is placed, inputting component action, transmitting a monitoring instruction to the controller, and starting a monitoring process when the controller enters a working state; the lifting assembly acts, the controller sends a control instruction to the water pump through the driver, and the shell 1 is suspended at different water depths in an injection or drainage mode so as to collect water samples at different water depths; after the sample is collected, the shell 1 floats to the water surface immediately so as to facilitate the normal work of the solar cell panel.
Furthermore, before ATP fluorescence monitoring of the water body sample, the fluorescence reaction tube is cleaned by using sterile water, and then the cleaning solution is injected into the waste liquid barrel. The controller sends control instructions to the sampling assembly and the reaction assembly, 1ml of water body sample is injected into the fluorescence reaction tube through the peristaltic pump 7, and then 50 microliters of lysate and 10 microliters of ATP (adenosine triphosphate) luciferase are added into the fluorescence reaction tube through the plunger pump; particularly, the reaction reagent needs to be refrigerated and stored, and needs to be placed at room temperature for more than 10 minutes.
After the reaction is finished, the controller sends a control instruction to the execution assembly to obtain a luminous value and a temperature value of the water body sample after the reaction, wherein the temperature value is used as an auxiliary parameter for evaluation and use, and the purpose is to reduce errors and contingency and further ensure the accuracy of the parameter; based on the firefly luminescence principle, Adenosine Triphosphate (ATP) is rapidly detected by using a luciferase-luciferin system, the ATP in cells is released by using a reagent which can crack cell membranes, the reaction is carried out on the ATP in the cells and a specific enzyme contained in the reagent to generate light, and then a luminescence value is monitored by using a photoelectric sensor; since the number of microorganisms is proportional to the luminescence value and all living cells of organisms contain a constant amount of ATP, the ATP content clearly indicates the concentration of microorganisms in the sample.
After the data reading is finished, the data is remotely transmitted to an upper computer of a worker through a GPRS communicator, so that the purpose of real-time online monitoring is achieved; during maintenance, corresponding reagents are added once every month, and manual assistance is eliminated to the maximum extent.
The monitoring frequency can be manually set through the clock component, so that the method is suitable for the diversity of parameter monitoring.
In summary, the system for monitoring the concentration of the microorganisms in the surface water source based on the ATP technology in the embodiment of the present invention is based on the integrated control function of the ATP fluorescence detection technology and the single chip microcomputer controller, and is matched with various types of electrical components and equipment components, and converts an optical signal into a corresponding electrical signal by using a bioluminescence principle after combining the microbial ATP and the ATP luciferase and a proportional relation between the luminous intensity and the ATP content, so as to achieve the purpose of monitoring the concentration of the microorganisms in real time, reduce the difficulty in acquiring monitoring data, make the monitoring data more intuitive and accurate, and avoid the generation of monitoring errors; meanwhile, the overall monitoring step and the actual working process are simplified, and the method is easy to popularize and popularize under different use scenes, so that the concentration parameters of microorganisms in different water areas can be accurately obtained.
The above-described embodiments should not be construed as limiting the scope of the invention, and any alternative modifications or alterations to the embodiments of the present invention will be apparent to those skilled in the art.
The present invention is not described in detail, but is known to those skilled in the art.
Claims (10)
1. ATP technology-based surface water source microorganism concentration monitoring system, characterized in that the monitoring system includes:
the device comprises a shell, a water tank, a water inlet pipe, a water outlet pipe, a water inlet pipe, a water outlet pipe and a water outlet pipe, wherein the shell is an oval shell and is used for being placed in a surface water source to be monitored to monitor the concentration parameters of microorganisms;
the controller is arranged in the shell and is electrically connected with a communication assembly, a clock assembly, an input assembly, an execution assembly, a sampling assembly, a lifting assembly, a power supply assembly and a reaction assembly; the communication assembly is used for establishing wireless network communication between the controller and the upper computer so as to remotely transmit monitoring parameters; the clock component is used for setting monitoring frequency; the input assembly is used for inputting a monitoring instruction to the controller to enable the controller to enter a working state; the sampling assembly is used for collecting a water body sample from a surface water source to be monitored; the lifting assembly is used for adjusting different positions of the shell in the surface water source so that the shell can be suspended at different depths in the surface water source, and sampling is convenient; the power supply assembly is used for providing stable and durable power supply voltage for the controller; the reaction assembly is used for injecting a reaction reagent into a water body sample; the execution assembly is used for monitoring a corresponding luminous value of the water body sample after reaction, converting the luminous value into a corresponding electric signal and inputting the electric signal into the controller so as to obtain a microorganism concentration parameter in the water body sample.
2. The ATP technology-based surface water source microorganism concentration monitoring system of claim 1, wherein: the model of controller is STM32F103C8T6, is equipped with 64 pins on the controller, the controller links to each other with the power supply subassembly through a pin, the controller links to each other with the input module through No. four pins, the controller links to each other with the executive component through No. fifteen pins, the controller links to each other with the communication subassembly through No. twenty pin, No. twenty-one pin and No. twenty-three pin, the controller links to each other with the reaction subassembly through No. thirty-three pin and No. thirty-four pin, the controller links to each other with lifting unit and sampling unit through No. thirty-eight pin, the controller links to each other with the clock subassembly through No. forty-five pin.
3. The ATP technology-based surface water source microorganism concentration monitoring system of claim 2, wherein: the power supply assembly comprises a solar cell panel, a voltage stabilizer and a lithium battery which are electrically connected, the solar cell panel is arranged at the top of the shell, the lithium battery is arranged at the bottom in the shell, and the voltage stabilizer is used for continuously and stably charging the lithium battery; the model of stabiliser is LM317, is equipped with three pin on the stabiliser, the stabiliser links to each other with solar cell panel through a pin, is equipped with fourth electric capacity and fifth resistance between a pin and No. two pins of stabiliser, is equipped with fifth electric capacity and sixth resistance between No. two pins and No. three pins of stabiliser, No. three pins of stabiliser link to each other with the lithium cell, No. one pin of lithium cell and controller links to each other.
4. The ATP technology-based surface water source microorganism concentration monitoring system of claim 2, wherein: the input assembly comprises a command input device, the type of the command input device is TLP290, four pins are arranged on the command input device, a first pin of the command input device is a monitoring command input pin, a ninth resistor, a tenth resistor and a fourth capacitor are connected in parallel between a first pin and a second pin of the command input device, a fifth capacitor and an eighth resistor are connected in parallel between a third pin and a fourth pin of the command input device, and a third pin of the command input device is connected with a fourth pin of the controller.
5. The ATP technology-based surface water source microorganism concentration monitoring system of claim 2, wherein: the execution assembly comprises an AD converter, a temperature sensor and a photoelectric sensor are connected to the AD converter, the temperature sensor is used for monitoring a temperature value of the water body sample after reaction, and the photoelectric sensor is used for monitoring a luminous value of the water body sample after reaction so as to obtain the microbial concentration of the water body sample; the AD converter is characterized in that the model of the AD converter is AD8551, 8 pins are arranged on the AD converter, the AD converter is connected with a No. fifteen pin of the controller through a No. six pin, and the AD converter is connected with the photoelectric sensor and the temperature sensor through a No. three pin; the model of the photoelectric sensor is OH-1021, and the model of the temperature sensor is SHT 20.
6. The ATP technology-based surface water source microorganism concentration monitoring system of claim 2, wherein: the communication component comprises a GPRS communicator, the model of the GPRS communicator is SIM800C, 42 pins are arranged on the GPRS communicator, a first pin of the GPRS communicator is connected with a twenty-first pin of the controller, a second pin of the GPRS communicator is connected with a twenty-first pin of the controller, a sixth pin of the GPRS communicator is connected with a twenty-second pin of the controller, and a sixth resistor and a seventh resistor which are connected in parallel are arranged between the second pin and the sixth pin of the GPRS communicator; the GPRS communicator is connected with an SIM card through a pin fifteen, a pin sixteen, a pin seventeen and a pin eighteen, a fourth capacitor, a fifth capacitor and a sixth capacitor are respectively arranged on the pin fifteen, the pin sixteen and the pin seventeen of the GPRS communicator, a seventh capacitor is connected to the pin eighteen of the GPRS communicator, and an antenna is connected to the pin thirty-two of the GPRS communicator.
7. The ATP technology-based surface water source microorganism concentration monitoring system of claim 2, wherein: the reaction assembly comprises a trigger and a plunger pump which are electrically connected, and the plunger pump is used for injecting a reaction reagent into a water body sample; the type of the trigger is FDS9945, the pin II of the trigger is connected with the pin thirty-eight of the controller, the pin III of the trigger is connected with the pin thirty-seven of the controller, and the pin eight and the pin five of the trigger are connected with a plunger pump.
8. The ATP technology-based surface water source microorganism concentration monitoring system of claim 2, wherein: the sampling assembly comprises a driver, a peristaltic pump and a linear push rod which are electrically connected, the peristaltic pump is used for sampling a water body sample and injecting the water body sample into the fluorescent reaction tube, and the linear push rod is used for adjusting the depth position of the peristaltic pump so as to sample at different depths of a surface water source; the linear push rod is vertically and downwards arranged at the bottom of the shell, and a piston rod of the linear push rod is provided with a peristaltic pump; the lifting assembly comprises a driver and a water pump which are electrically connected, and the water pump is arranged in the water injection bins at two ends of the bottom of the shell so as to adjust the mass of water in the water injection bins to change the depth of the shell; the driver is of the type ULN2003, 16 pins are arranged on the driver, the driver is connected with thirty-eight pins of the controller through a pin I, a first relay is connected to a pin sixteen of the driver, a first resistor and a first diode which are connected in parallel are arranged on the first relay, and an equipment interface is arranged on the first relay and used for electrically connecting the peristaltic pump, the linear push rod and the water pump.
9. The ATP technology-based surface water source microorganism concentration monitoring system of claim 2, wherein: the clock assembly comprises a timer, the type of the timer is DS1302, 8 pins are arranged on the timer, a fourth resistor and a fourth capacitor which are connected in parallel are arranged between a sixth pin and a seventh pin of the timer, and the seventh pin of the timer is connected with a forty-five pin of the controller.
10. The ATP technology-based surface water source microorganism concentration monitoring system of claim 2, wherein: the two ends of the shell are respectively provided with a limiting rubber pad, and the limiting rubber pads are used for keeping the shell in a vertical position, so that the shell is not deviated and is prevented from colliding with the external environment; a proximity switch is arranged in each limit rubber pad, the model of the proximity switch is FCS004, and the proximity switch is connected with a forty-six pin of the controller; and a stabilizing bracket and a positioning anchor are also arranged at the bottom of the shell.
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| CN202111468298.5A CN114167780A (en) | 2021-12-03 | 2021-12-03 | Surface water source microorganism concentration monitoring system based on ATP technology |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115261213A (en) * | 2022-08-17 | 2022-11-01 | 山东师范大学 | Portable airborne microorganism rapid monitoring device based on automatic control transmission feedback principle |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206848739U (en) * | 2017-04-10 | 2018-01-05 | 杭州腾海科技有限公司 | A kind of pond ecological environment online monitoring system |
| CN112690925A (en) * | 2020-12-28 | 2021-04-23 | 山东省农业科学院畜牧兽医研究所 | Special automatic gastric lavage device for rabbit experiment |
| CN213484588U (en) * | 2020-12-17 | 2021-06-18 | 李锡刚 | Regulator cubicle data remote transmission communication device |
| CN113406425A (en) * | 2021-07-16 | 2021-09-17 | 山东中呈防雷科技有限公司 | Three-dimensional thunder and lightning real-time supervision early warning system |
| CN214307325U (en) * | 2021-03-05 | 2021-09-28 | 青岛世纪信科仪表科技有限公司 | Wisdom heat supply control system |
| CN214424822U (en) * | 2021-02-24 | 2021-10-19 | 赵冬亮 | Communication fault diagnosis system for lime kiln hydraulic station |
| CN214795673U (en) * | 2021-07-22 | 2021-11-19 | 山东鑫弘重工有限公司 | Full-automatic unattended non-stop oil stringing machine |
| CN214794012U (en) * | 2021-05-27 | 2021-11-19 | 合肥欧沃环保科技有限公司 | Water sampling device of adjustable sampling depth |
-
2021
- 2021-12-03 CN CN202111468298.5A patent/CN114167780A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206848739U (en) * | 2017-04-10 | 2018-01-05 | 杭州腾海科技有限公司 | A kind of pond ecological environment online monitoring system |
| CN213484588U (en) * | 2020-12-17 | 2021-06-18 | 李锡刚 | Regulator cubicle data remote transmission communication device |
| CN112690925A (en) * | 2020-12-28 | 2021-04-23 | 山东省农业科学院畜牧兽医研究所 | Special automatic gastric lavage device for rabbit experiment |
| CN214424822U (en) * | 2021-02-24 | 2021-10-19 | 赵冬亮 | Communication fault diagnosis system for lime kiln hydraulic station |
| CN214307325U (en) * | 2021-03-05 | 2021-09-28 | 青岛世纪信科仪表科技有限公司 | Wisdom heat supply control system |
| CN214794012U (en) * | 2021-05-27 | 2021-11-19 | 合肥欧沃环保科技有限公司 | Water sampling device of adjustable sampling depth |
| CN113406425A (en) * | 2021-07-16 | 2021-09-17 | 山东中呈防雷科技有限公司 | Three-dimensional thunder and lightning real-time supervision early warning system |
| CN214795673U (en) * | 2021-07-22 | 2021-11-19 | 山东鑫弘重工有限公司 | Full-automatic unattended non-stop oil stringing machine |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115261213A (en) * | 2022-08-17 | 2022-11-01 | 山东师范大学 | Portable airborne microorganism rapid monitoring device based on automatic control transmission feedback principle |
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