CN114166586A - Device and method for sampling plant volatile organic compounds - Google Patents
Device and method for sampling plant volatile organic compounds Download PDFInfo
- Publication number
- CN114166586A CN114166586A CN202111654499.4A CN202111654499A CN114166586A CN 114166586 A CN114166586 A CN 114166586A CN 202111654499 A CN202111654499 A CN 202111654499A CN 114166586 A CN114166586 A CN 114166586A
- Authority
- CN
- China
- Prior art keywords
- air
- bag
- gas
- volatile organic
- organic compounds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/24—Suction devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/02—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols by adsorption, e.g. preparative gas chromatography
- B01D53/04—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols by adsorption, e.g. preparative gas chromatography with stationary adsorbents
- B01D53/0407—Constructional details of adsorbing systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2257/00—Components to be removed
- B01D2257/10—Single element gases other than halogens
- B01D2257/106—Ozone
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2257/00—Components to be removed
- B01D2257/70—Organic compounds not provided for in groups B01D2257/00 - B01D2257/602
- B01D2257/708—Volatile organic compounds V.O.C.'s
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
A device for sampling plant volatile organic compounds belongs to the technical field of gas sampling. The device includes the air pocket body and is used for fixing the mounting on the plant branch with the air pocket body, its characterized in that: the air bag body consists of a gas enrichment bag and an inflatable shaping bag fixedly connected with the outer side wall of the gas enrichment bag; the gas enrichment bag comprises a cylindrical first cavity, a circular truncated cone-shaped second cavity and an opening, and a gas outlet is formed in the circular bottom surface of the first cavity; an inflation opening is formed in the side wall of the inflation shaping bag; the fixing piece is a sealing clip. The portable outdoor sampler is ingenious in design, convenient to fold and carry, and particularly has great advantages in field sampling.
Description
Technical Field
The invention belongs to the technical field of gas sampling, particularly relates to a device for sampling plant volatile organic compounds, and provides a method for collecting the plant volatile organic compounds by using the device.
Background
Plant volatile organic compounds (BVOCs) are low-boiling-point volatile small-molecular organic compounds synthesized by plants through a secondary metabolic pathway, and mainly comprise terpenes, hydrocarbons, aldehydes, alcohols, esters, ketones, acids and the like. Plant volatile organic compounds are mainly produced by leaves and released into the atmosphere, and have important influences on air quality, atmospheric environment, carbon circulation, human physical and psychological health and the like.
The factors influencing the release of the plant volatile organic compounds mainly include plant self factors and external environment factors, wherein: the plant factors include the species, growth state and development stage of the plant, and the tree species difference is the primary factor determining the release of volatile organic compounds from trees, and is the essential difference between trees, which plays an important role in the composition and release rate of the volatile organic compounds from the plant. The method is used for determining the chemical components and release rates of volatile organic compounds released by different tree species under specific environmental conditions, which is the basis for developing research on the volatile organic compounds, so that the volatile organic compounds of plants need to be sampled and measured.
The sampling method of the volatile organic compounds released by the plant leaves mainly comprises a static closed sampling method and a dynamic headspace sampling method, wherein the two methods are to lock branches, leaves or the whole plant of a certain outdoor tree species for sampling, and then bring a sampling adsorption tube back to a laboratory to measure the components and the content of the volatile organic compounds by using an analytical instrument. The static closed sampling method is characterized in that branch leaves of a tree to be tested are covered in an air bag, after the interval time is set, the gas in the air bag is collected at the interval time point, and the gas is measured by an analytical instrument after being collected. In order to overcome the technical defect, in recent years, a dynamic headspace method is mainly adopted, which is also a method widely applied at present for measuring volatile organic compounds emitted by living plants, different from the static closed sampling method, the method has the advantages that the two ends of the air bag are opened, the natural flow of air in the air bag is kept, the gas in the air bag is guided into the adsorption tube or the air bag by the air pumping of the sampling pump for sampling, then the on-machine detection and the data analysis in a laboratory are carried out, wherein, the release rate needs to be calculated according to the parameters such as the change of the gas concentration, the used time interval, the leaf area (or leaf weight) closed in the air bag, the air bag volume and the like, however, because the air bag is made of transparent soft materials and has no fixed shape after being inflated, the volume of the air bag is closely related to the position of each tying, the flow rate of the air pump and the like, so that the volume of the air bag is difficult to accurately calculate during sampling, and the accurate calculation of the release rate cannot be realized. Based on the technical defect, the method for sampling the dynamic headspace by using the air bag can only be used for qualitative and relative quantification, and absolute quantification is inaccurate. In order to accurately quantify, scientific research personnel try to adopt a glass container with a fixed volume for sampling, but the glass container is heavy and fragile, is inconvenient to carry, needs a long balance time, is not suitable for sampling field plant living bodies, and can only be used for sampling potted seedlings in a laboratory.
Disclosure of Invention
In view of the above technical problems in the background art, the present invention is directed to a gas bag for sampling plant volatile organic compounds in vivo, which is suitable for both laboratories and the field.
In order to achieve the purpose, the invention adopts the following technical scheme:
device of plant volatile organic compounds sampling, including the air pocket body with be used for fixing the mounting on the plant branch with the air pocket body, its characterized in that: the air bag body consists of a gas enrichment bag and an inflatable shaping bag fixedly connected with the outer side wall of the gas enrichment bag; the gas enrichment bag comprises a cylindrical first cavity, a circular truncated cone-shaped second cavity and an opening, and a gas outlet is formed in the circular bottom surface of the first cavity; an inflation opening is formed in the side wall of the inflation shaping bag; the fixing piece is a sealing clip.
The device of plant volatile organic compounds sampling, its characterized in that: the sealing clamp comprises a first clamping strip provided with a groove and a second clamping strip provided with a convex strip, and a first through hole and a second through hole are further formed in the middle of the sealing clamp.
The device of plant volatile organic compounds sampling, its characterized in that: the cross section of the second holding strip is in an omega shape, and the second holding strip is matched with the first holding strip.
The device of plant volatile organic compounds sampling, its characterized in that: a first sealing washer consisting of two semicircular rings is arranged on the wall of the first through hole; and a second sealing washer consisting of two semicircular rings is arranged on the wall of the second through hole.
The device of plant volatile organic compounds sampling, its characterized in that: and one end of each of the first clamping strip and the second clamping strip is provided with a connecting piece.
The device of plant volatile organic compounds sampling, its characterized in that: the device also comprises an air purification system, wherein the air purification system comprises an air inlet pipe, an adsorption column and an ozone removal column, the adsorption column comprises a first adsorption column and a second adsorption column, and a valve is arranged at one end, close to the air bag body, of the air inlet pipe; the first adsorption column is filled with a mixture of silica gel and activated carbon; and the second adsorption column is filled with materials for adsorbing volatile organic compounds.
The device of plant volatile organic compounds sampling, its characterized in that: the second adsorption column is filled with a material for adsorbing volatile organic compounds, namely a carbon molecular plug.
The device of plant volatile organic compounds sampling, its characterized in that: the adsorption column comprises a column body, a column cover, a gas inlet and a gas outlet, wherein a partition plate is arranged in the longitudinal middle of the column body, and the column body is divided into two semi-cylindrical cavities through the partition plate; the gas inlet and the gas outlet are positioned in two different semi-cylindrical cavities; wherein the gas outlet is positioned at the bottom surface of the column body, and the gas inlet is positioned at the side wall of the column body.
The device of plant volatile organic compounds sampling, its characterized in that: the device also comprises an air extraction and enrichment system, wherein the air extraction and enrichment system comprises an air outlet pipe, an adsorption pipe and an air extraction pump, and a valve is arranged at one end of the air outlet pipe close to the air bag body; one end of the air outlet pipe is connected with the air outlet.
The method for sampling the volatile organic compounds of the plants is characterized by comprising the following steps:
1) preparing the plant volatile organic compound sampling device;
2) inflating the inflatable shaping bag through an inflation port to 1/3-2/3 in a saturated state;
3) selecting a branch to be detected, and inserting the branch into a gas enrichment bag from an opening;
4) preparing a sealing clamp, aligning the branches with a second through hole of the sealing clamp, wherein the first through hole is used for placing an air inlet pipe, the sealing clamp clamps an opening, a valve A is arranged at one end of the air inlet pipe close to the air bag, and the valve A is adjusted to be in an off state; in order to make the air entering the gas enrichment bag be clean air, the other end of the air inlet pipe is sequentially connected with a second absorption column, an ozone removal column and a first absorption column;
5) connecting an air outlet pipe with an air outlet, wherein one end of the air outlet pipe close to the air outlet is provided with a valve B, and the valve B is adjusted to be in an 'on' state;
6) opening the inflation inlet to discharge the gas in the inflation shaping bag;
7) slightly pressing the air bag to discharge residual air in the air enrichment bag from the air outlet;
8) adjusting the valve A to be in an 'on' state, and adjusting the valve B to be in an 'off' state;
9) slowly inflating the inflatable shaping bag from the inflating port to a saturated state, and sucking clean air from the air inlet by the gas enrichment bag;
10) the outlet end of the gas outlet pipe is connected with a gas production pump, the valve B is adjusted to be in an 'on' state, and the gas production pump is started until the gas in the gas enrichment bag reaches a balance state; then an adsorption pipe is connected to the air inlet end of the air extraction pump, and the air extraction pump is started to sample the plant volatile organic compounds.
The device for sampling the plant volatile organic compounds, which is designed by the invention, has the following beneficial effects: (1) the air bag is made of soft transparent materials, air in the air bag is emptied during transportation, and the air bag is foldable, light and handy and convenient to carry, so that the technical defects that materials such as glass are fragile and heavy are overcome. (2) The air bag body comprises gas enrichment bag and rather than the inflation design bag of lateral wall fixed connection, during the use, is full of gas through the inflation inlet and aerifys the design bag, and along with aerifing going on, gas enrichment bag slowly swells, finally reaches full state, maintains its fixed shape to the realization can accurately calculate the volume of air in the air bag for calculate the release rate of blade volatility organic matter, realize accurate ration. (3) The sealing clamp is composed of 2 clamping strips with special structures, the groove of the first clamping strip is matched with the convex strip of the second clamping strip, and the 2 pairs of through holes in the middle are used for accommodating branches and air inlet pipelines respectively, so that the air bag can be fixed on the branches, and the branches can be sealed in the air bag. (4) According to the air purification system provided by the invention, when the adsorption column with a special structure is designed and the filler in the optimized scheme is filled, the purpose of removing ozone, water vapor and Volatile Organic Compounds (VOCs) in ambient air can be realized, after the air in the external environment passes through the air purification system provided by the invention, tests show that neither ozone nor VOCs is detected, and the air purification effect is good.
Drawings
FIG. 1 is a schematic view of the construction of the airbag body and the sealing clip of the present invention;
FIG. 2 is a schematic view of the structure of the airbag body according to the present invention;
FIG. 3 is a schematic view of a first clip strip of the enclosure;
FIG. 4 is a schematic view of a second clip strip of the enclosure;
FIG. 5 is a schematic view of the structure of the sealing clip in a semi-closed state;
FIG. 6 is a schematic view of the closed state of the sealing clip;
FIG. 7 is a right side view of FIG. 6;
FIG. 8 is a schematic structural view of the present invention in use;
FIG. 9 is a schematic view of the structure of the adsorption column;
in the figure: 1-gas enrichment bag; 1-1-a first cavity; 1-2-a second cavity; 1-3-opening; 1-4-gas outlet; 2-inflating and shaping bags; 2-1-an inflation inlet; 3-sealing the clamp; 3-1-a first holding strip; 3-1A groove; 3-2-a second holding strip; 3-2B-ridges; 3-3-a first via; 3-4-a second via; 3-3 s-a first sealing gasket; 3-4 s-second sealing gasket; 3-5-connectors; 4-branches; 5-an air inlet pipe, 5-1-a valve A; 6-a second absorption column; 7-an ozone removal column; 8-a first absorption column; 9-air outlet pipe, 9-1-valve B; 10-an adsorption tube; 11-a gas extraction pump. C1-column shaft; c2-column cover; c3-gas inlet; c4 — gas outlet; c5-separator.
Detailed Description
The present invention will be described in further detail below with reference to the accompanying drawings, which are not specifically described in the prior art.
The invention designs a plant volatile organic compound sampling device (figure 9), which comprises an air bag body and a fixing part for fixing the air bag body on plant branches, wherein the air bag body designed by the invention comprises an inner bag and an outer bag, as shown in figure 2, the inner bag is a gas enrichment bag 1 and is used for accommodating branches and leaves and enriching gaseous substances released by the branches and leaves; the outer bag is the inflation shaping bag 2, the inner wall of the gas enrichment bag 1 and the side wall of the inflation shaping bag 2 are in the same wall to realize the fixed connection of the outer bag and the inner bag, and the inflation shaping bag 2 is inflated to drive the gas enrichment bag 1 to swell, so that the fixed shape and the volume of the gas enrichment bag 1 are realized. The gas enrichment bag 1 comprises a cylindrical first cavity 1-1, a truncated cone-shaped second cavity 1-2 and an opening 1-3, and the circular bottom surface of the first cavity is provided with a gas outlet 1-4; the side wall of the inflatable shaping bag 2 is provided with an inflation inlet 2-1.
In order to realize the sealing and fixing of the branches and leaves to be tested in the gas enrichment bag 1, the invention designs a sealing clip 3 (figures 1, 3-7), the sealing clip 3 comprises a first clip strip 3-1 and a second clip strip 3-2, wherein the longitudinal middle part of the first holding strip 3-1 is provided with a groove 3-1A, the longitudinal middle part of the second holding strip 3-2 is provided with a convex strip 3-2B which protrudes upwards, for better sealing and fastening, the ribs are arched, preferably in the range of 200-300, namely, the cross section of the second holding strip 3-2 is in an omega shape, the shape and the size of the convex strip are matched with those of the groove, the second holding strip 3-2 is embedded into the groove 3-1A of the first holding strip 3-1 through the convex strip 3-2B, the closing of the sealing clamp is realized, and the closed state is shown in figures 6 and 7. The middle part of the sealing clamp 3 is also provided with 2 pairs of through holes, namely a first through hole 3-3 and a second through hole 3-4, wherein the second through hole 3-4 is used for accommodating the branch 4 and fixing the branch 4 in the second through hole 3-3, the first through hole 3-3 is used as an air inlet, air outside the air bag enters the gas enrichment bag 1 from the air inlet, and the functions of the first through hole 3-3 and the second through hole 3-4 can be interchanged. In order to realize better sealing effect and not damage branches, a first sealing washer 3-3s consisting of two pairs of semicircular rings is arranged on the wall of the first through hole 3-3; the hole wall of the second through hole 3-4 is provided with a second sealing washer 3-4s formed by two pairs of half rings, and it should be noted that the middle part of the second holding strip 3-2 has no convex strip, that is, there is no convex strip between the first sealing washer and the second sealing washer located at both sides of the convex strip (see fig. 4).
Preferably, one end of the first holding strip 3-1 and the second holding strip 3-2 is preferably provided with a connecting member 3-5, so that the alignment and matching of the first holding strip 3-1 and the second holding strip 3-2 can be better achieved.
Since most plant volatile substances are released depending on light, the air bag for collecting plant volatile organic compounds needs to be made of a light-transmitting material, and the air bag for enriching gas needs to be made of a material which does not release volatile organic compounds and absorbs little or no volatile organic compounds released by plants, such as a film bag made of polytetrafluoroethylene material.
In order to make the air entering the gas enrichment bag be clean air, the device designed by the invention also comprises an air purification system, wherein the air purification system comprises an air inlet pipe 5, an adsorption column and an ozone removal column 7, the adsorption column comprises a first adsorption column 8 and a second adsorption column 6, and one end of the air inlet pipe close to the air bag body is provided with a valve A5-1. The adsorption column of the invention has the following structure: the device comprises a column body C1, a column cover C2, a gas inlet C3 and a gas outlet C4, wherein a partition plate C5 is arranged at the longitudinal middle part (the plane of a longitudinal central line) of the column body C1, and the column body is divided into two semi-cylindrical cavities by the partition plate; the gas inlet and the gas outlet are positioned in two different semi-cylindrical cavities; wherein gas outlet is located the shaft bottom surface, and gas inlet is located the shaft lateral wall, and concrete structure is as shown in fig. 9, and column lid C2 is down (as figure 8) during the use, and column lid C2 passes through threaded connection with shaft C1, and the purpose that the unique structure of adsorption column can realize the better adsorption effect of shaft interior filler and extension adsorption material life.
The air inlet pipe 5 is connected with a second absorption column 6 (the absorption column is filled with a filler for adsorbing volatile organic compounds in air, preferably, a semi-cylinder cavity of the second absorption column communicated with a gas inlet C3 is filled with a round granular carbon molecular plug, the semi-cylinder cavity communicated with a gas outlet C4 is filled with a mixture of Tenex, Carbograph1 TD and Carboxex1003, and the mass ratio of the Tenex, the Carbograph1 TD and the Carboxex1003 is preferably 2:1: 1), an ozone removal column 7 (for removing ozone) and a first absorption column 8 (for adsorbing water vapor and part of volatile organic compounds, preferably a mixture of silica gel and activated carbon) in sequence.
The method for collecting the plant volatile organic compounds by adopting the device to carry out the dynamic headspace sampling method comprises the following steps:
1) preparing the article of FIG. 8;
2) inflating the inflatable shaping bag 2 through the inflation inlet 2-1 to 1/3-2/3 in a saturated state;
3) selecting a branch 4 to be tested, and carefully inserting the branch into the gas enrichment bag 1 from the opening 1-3;
4) preparing a sealing clamp, aligning the branches with a second through hole of the sealing clamp, wherein the first through hole is used for placing an air inlet pipe, the sealing clamp clamps an opening, a valve A is arranged at one end of the air inlet pipe close to the air bag, and the valve A is adjusted to be in an off state; in order to make the air entering the gas enrichment bag be clean air, the other end of the air inlet pipe is sequentially connected with a second absorption column, an ozone removal column and a first absorption column;
5) connecting an air outlet pipe 9 with an air outlet 1-4, wherein one end of the air outlet pipe 9 close to the air outlet 1-4 is provided with a valve B9-1, and the valve B is adjusted to be in an 'on' state;
6) opening the inflation inlet to discharge the gas of the inflatable shaping bag 2;
7) slightly pressing the air bag to discharge residual air in the gas enrichment bag 1 from the air outlet 1-4;
8) adjusting the valve A to be in an 'on' state, and adjusting the valve B to be in an 'off' state;
9) slowly inflating the inflatable shaping bag 2 from the inflating port 2-1 to a saturated state, and then sucking clean air from the air inlet by the gas enrichment bag 1;
10) the outlet end of the gas outlet pipe 9 is connected with a gas production pump 11, the valve B is adjusted to be in an 'on' state, and the gas production pump 11 is started until the gas in the gas enrichment bag 1 reaches a balance state; then, an adsorption pipe 10 is connected to the air inlet end of the gas production pump 11, and the gas production pump 11 is started to sample plant volatile organic compounds; at the moment, the volume of the gas enrichment bag 1 is equal to the sum of the volume of the cylindrical first cavity 1-1 and the truncated cone-shaped second cavity 1-2.
The air bag designed by the invention has the following beneficial effects: (1) the air bag is made of soft transparent materials, and the air bag is emptied of air during transportation, can be folded, is light and handy, is convenient to carry, and solves the technical defect that materials such as glass are fragile and heavy. (2) The air bag body comprises gas enrichment bag and rather than the inflation design bag of lateral wall fixed connection, during the use, is full of gas through the inflation inlet and aerifys the design bag, and along with aerifing going on, gas enrichment bag slowly swells, finally reaches full state, maintains its fixed shape to the volume that realizes can accurately calculating the air in the air bag is used for calculating the release rate of blade volatility organic matter, realizes accurate ration. (3) The sealing clamp is composed of 2 clamping strips with special structures, the groove of the first clamping strip is matched with the convex strip of the second clamping strip, and the 2 pairs of through holes in the middle are used for accommodating branches and air inlet pipelines respectively, so that the air bag can be fixed on the branches, and the branches can be sealed in the air bag. (4) According to the air purification system provided by the invention, when the adsorption column with a special structure is designed and the filler in the optimized scheme is filled, the purpose of removing ozone, water vapor and Volatile Organic Compounds (VOCs) in ambient air can be realized, after the air in the external environment passes through the air purification system provided by the invention, tests show that neither ozone nor VOCs is detected, and the air purification effect is good.
Claims (10)
1. Device of plant volatile organic compounds sampling, including the air pocket body with be used for fixing the mounting on the plant branch with the air pocket body, its characterized in that: the air bag body consists of a gas enrichment bag (1) and an inflatable shaping bag (2) fixedly connected with the outer side wall of the gas enrichment bag; the gas enrichment bag (1) comprises a cylindrical first cavity (1-1), a truncated cone-shaped second cavity (1-2) and an opening (1-3), and a gas outlet (1-4) is formed in the circular bottom surface of the first cavity; an inflation opening (2-1) is arranged on the side wall of the inflation shaping bag (2); the fixing piece is a sealing clip (3).
2. The apparatus for sampling plant volatile organic compounds according to claim 1, wherein: the sealing clamp (3) comprises a first clamping strip (3-1) provided with a groove (3-1A) and a second clamping strip (3-2) provided with a convex strip (3-2B), and a first through hole (3-3) and a second through hole (3-4) are further formed in the middle of the sealing clamp.
3. The apparatus for sampling plant volatile organic compounds according to claim 2, wherein: the cross section of the second holding strip (3-2) is in an omega shape, and the second holding strip is matched with the first holding strip (3-1).
4. The apparatus for sampling plant volatile organic compounds according to claim 2, wherein: a first sealing washer (3-3 s) consisting of two semicircular rings is arranged on the wall of the first through hole (3-3); and a second sealing washer (3-4 s) consisting of two semicircular rings is arranged on the wall of the second through hole (3-4).
5. The apparatus for sampling plant volatile organic compounds according to claim 2, wherein: one end of the first holding strip (3-1) and one end of the second holding strip (3-2) are provided with connecting pieces (3-5).
6. The apparatus for sampling plant volatile organic compounds according to claim 1, wherein: the device also comprises an air purification system, wherein the air purification system comprises an air inlet pipe (5), an adsorption column and an ozone removal column (7), the adsorption column comprises a first adsorption column (8) and a second adsorption column (6), and one end, close to the air bag body, of the air inlet pipe (5) is provided with a valve A (5-1); the first adsorption column (8) is filled with a mixture of silica gel and activated carbon; the second adsorption column (6) is filled with materials for adsorbing volatile organic compounds.
7. The apparatus for sampling plant volatile organic compounds according to claim 6, wherein: the second adsorption column (6) is filled with a material for adsorbing volatile organic compounds, and the material is a carbon molecular plug.
8. The apparatus for sampling plant volatile organic compounds according to claim 6, wherein: the adsorption column comprises a column body (C1), a column cover (C2), a gas inlet (C3) and a gas outlet (C4), wherein a partition plate (C5) is arranged in the longitudinal middle of the column body (C1), and the column body is divided into two semi-cylindrical cavities by the partition plate; the gas inlet and the gas outlet are positioned in two different semi-cylindrical cavities; wherein the gas outlet is positioned at the bottom surface of the column body, and the gas inlet is positioned at the side wall of the column body.
9. The apparatus for sampling plant volatile organic compounds according to claim 1, wherein: the device also comprises an air extraction and enrichment system, wherein the air extraction and enrichment system comprises an air outlet pipe (9), an adsorption pipe (10) and an air extraction pump (11), and one end of the air outlet pipe (9), which is close to the air bag body, is provided with a valve B (9-1); one end of the air outlet pipe (9) is connected with the air outlet (1-4).
10. The method for sampling the volatile organic compounds of the plants is characterized by comprising the following steps:
1) means for preparing a plant volatile organic compound sample according to any one of claims 1 to 9;
2) inflating the inflatable shaping bag through an inflation port to 1/3-2/3 in a saturated state;
3) selecting a branch to be detected, and inserting the branch into a gas enrichment bag from an opening;
4) preparing a sealing clamp, aligning the branches with a second through hole of the sealing clamp, wherein the first through hole is used for placing an air inlet pipe, the sealing clamp clamps an opening, a valve A is arranged at one end of the air inlet pipe close to the air bag, and the valve A is adjusted to be in an off state; in order to make the air entering the gas enrichment bag be clean air, the other end of the air inlet pipe is sequentially connected with a second absorption column, an ozone removal column and a first absorption column;
5) connecting an air outlet pipe with an air outlet, wherein one end of the air outlet pipe close to the air outlet is provided with a valve B, and the valve B is adjusted to be in an 'on' state;
6) opening the inflation inlet to discharge the gas in the inflation shaping bag;
7) slightly pressing the air bag to discharge residual air in the air enrichment bag from the air outlet;
8) adjusting the valve A to be in an 'on' state, and adjusting the valve B to be in an 'off' state;
9) slowly inflating the inflatable shaping bag from the inflating port to a saturated state, and sucking clean air from the air inlet by the gas enrichment bag;
10) the outlet end of the gas outlet pipe is connected with a gas production pump, the valve B is adjusted to be in an 'on' state, and the gas production pump is started until the gas in the gas enrichment bag reaches a balance state; then an adsorption pipe is connected to the air inlet end of the air extraction pump, and the air extraction pump is started to sample the plant volatile organic compounds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111654499.4A CN114166586A (en) | 2021-12-31 | 2021-12-31 | Device and method for sampling plant volatile organic compounds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111654499.4A CN114166586A (en) | 2021-12-31 | 2021-12-31 | Device and method for sampling plant volatile organic compounds |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114166586A true CN114166586A (en) | 2022-03-11 |
Family
ID=80488716
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111654499.4A Pending CN114166586A (en) | 2021-12-31 | 2021-12-31 | Device and method for sampling plant volatile organic compounds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114166586A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117367899A (en) * | 2023-12-08 | 2024-01-09 | 河北科技大学 | Portable living plant VOCs releases sampling device |
-
2021
- 2021-12-31 CN CN202111654499.4A patent/CN114166586A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117367899A (en) * | 2023-12-08 | 2024-01-09 | 河北科技大学 | Portable living plant VOCs releases sampling device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Fitzgerald et al. | Subnanogram determination of mercury by two-stage gold amalgamation and gas phase detection applied to atmospheric analysis | |
| Cocheo et al. | High uptake rate radial diffusive sampler suitable for both solvent and thermal desorption | |
| Raich et al. | Comparison of two static chamber techniques for determining carbon dioxide efflux from forest soils | |
| Mochalski et al. | Suitability of different polymer bags for storage of volatile sulphur compounds relevant to breath analysis | |
| Ryden et al. | Direct in‐field measurement of nitrous oxide flux from soils | |
| US3676073A (en) | Alveolar breath volumetric analysis for alcohol | |
| Leck et al. | Determination of reduced sulfur compounds in aqueous solutions using gas chromatography-flame photometric detection | |
| CN114166586A (en) | Device and method for sampling plant volatile organic compounds | |
| CN105486552B (en) | The lasting active in-situ acquisition system of class organic pollution in a kind of gaseous phase of soil | |
| CN1258079C (en) | Method of sampling greenhouse gas by utilizing gas balance bag | |
| CN202886148U (en) | Sampling collector of organic volatile matters in product/sample | |
| Jayanty | Evaluation of sampling and analytical methods for monitoring toxic organics in air | |
| CN217059554U (en) | Air bag for sampling plant volatile organic compounds in vivo | |
| Huang et al. | Evaluation and application of a passive air sampler for atmospheric volatile organic compounds | |
| Lenfant et al. | Measurement of blood gases by gas chromatography | |
| CN201025473Y (en) | Portable sorbent and inspiration measurement instrument | |
| CN101216385A (en) | N15/14 isotopic abundance sample collection method and apparatus for determining plant discharging N2O | |
| Magnusson | A method for equilibration chamber sampling and gas Chromatographie analysis of the soil atmosphere | |
| CN108387649A (en) | Detection method that is a kind of while detecting a variety of pernicious gases in workplace | |
| CN207408359U (en) | External renewable formula the drying plant of carrier gas in a kind of gas chromatograph examination | |
| CN108444536A (en) | The measurement device and method of portable refuse landfill sulfurous gas discharge capacity | |
| CN210626421U (en) | Portable flux box for measuring greenhouse gas emission flux of drainage pipe network | |
| Seiler et al. | Chromatography of Dns derivatives on pre-coated high-performance thin-layer chromatographic plates | |
| CN210269312U (en) | Volatile organic compound gas sampling device of plant leaf | |
| KR100735122B1 (en) | Sampling apparatus for collecting ambient voc |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |