CN210269312U - Volatile organic compound gas sampling device of plant leaf - Google Patents
Volatile organic compound gas sampling device of plant leaf Download PDFInfo
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- CN210269312U CN210269312U CN201920803128.XU CN201920803128U CN210269312U CN 210269312 U CN210269312 U CN 210269312U CN 201920803128 U CN201920803128 U CN 201920803128U CN 210269312 U CN210269312 U CN 210269312U
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Abstract
The embodiment of the utility model provides a gaseous collection system of plant leaf, concretely relates to gaseous sampling device of volatile organic compound of plant leaf. The utility model provides a gaseous sampling device of volatile organic compound of plant leaf, include: a portable photosynthetic analyzer, the portable photosynthetic analyzer comprising: the gas-liquid separation device comprises a control element, a working element, a vane chamber, a first gas path pipeline and a second gas path pipeline, wherein the first gas path pipeline is used for communicating a gas outlet of the control element with a gas inlet of the vane chamber; the second gas path pipeline is also communicated with a third gas path pipeline, and a first switching valve, an adsorption pipe, a first flow controller and an air pump are sequentially arranged on the third gas path pipeline along the gas flowing direction. The utility model provides a VOCs gas sampling device that the environment is easily controlled, do not receive interference, the reliable and stable plant leaf release of gas circuit of high concentration VOCs in the air.
Description
Technical Field
The utility model relates to a gaseous collection system of plant leaf, concretely relates to gaseous sampling device of volatile organic compound of plant leaf.
Background
Plants, as an important component of the ecosystem, can fix carbon dioxide and release oxygen, and can release Volatile Organic Compounds (VOCs) from leaves, the released volatile organic compounds have strong atmospheric reactivity, can rapidly participate in photochemical reaction under certain meteorological conditions such as illumination and the like, and have important influence on the formation of ozone and secondary aerosol in the atmosphere, and the influence degree generally has a positive correlation with the total emission amount.
In recent years, in order to collect as much as possible the volatile organic compounds released by the leaves of plants in the natural state, the relevant researchers developed a dynamic headspace collection method: normally, healthy leaves without diseases and insect pests are selected to carry out dynamic headspace bagging of living plants, then the bags are taken down, collected gas is pumped into an adsorption tube through an air pump, and the concentration of volatile organic compounds released by the plant leaves is analyzed.
Although the method is simple and rapid, the environmental conditions around the blades are difficult to control, the standard discharge rate of the VOCs needs to be statistically derived through a correlation formula, and statistical errors cannot be avoided. In addition, in the adsorption process, the ambient air is used as the carrier gas, but at present, the urban air usually contains higher-concentration VOCs, the adsorption capacity of the adsorption tube is easily exceeded if the adsorption time is a little longer, and the release of the plant VOCs is not easy to reach stability if the adsorption time is too short.
The portable photosynthetic determinator is an instrument for determining the photosynthetic rate of a plant by detecting the consumption rate of carbon dioxide under the condition of accurately controlling environmental factors, and comprises a leaf chamber, a control element and a working element;
regarding the illumination, temperature and humidity, the control element, the working element and the leaf chamber are regulated in the following way:
the inside of the leaf chamber is provided with: the illumination sensor and the temperature and humidity sensor are respectively and electrically connected with the control element and are respectively used for transmitting an illumination intensity signal and a temperature and humidity signal in the leaf chamber to the control element;
the control element is electrically connected with the working element and is used for controlling the light source intensity, the temperature control resistance value and the humidity regulator in the working element;
the working element is communicated with the leaf chamber, a light source, a temperature control resistor and a humidity regulator are arranged in the working element, and the control element controls the working element so as to adjust the illumination intensity, the temperature and the humidity in the leaf chamber;
with regard to the gas, the control element, the working element and the leaf chamber are regulated in the following manner:
the control element is provided with an external gas inlet, a control element gas outlet and a control element gas inlet, the external gas inlet is communicated with external gas, the control element gas outlet is communicated with the leaf chamber gas inlet through a gas path pipeline, and the control element gas inlet is communicated with the leaf chamber gas outlet through another gas path pipeline; the control element is also internally provided with CO2Small steel cylinder of the CO2CO in small steel cylinder2The gas outlet can enter the vane cell through the control element.
The gas in the vane chamber comes from the gas outlet of the control element, namely the gas in the vane chamber comprises external gas and CO from the control element2CO in small steel cylinder2. The gas in the vane chamber is returned to the control element through another gas path pipeline, and the gas signal is fed back to the control element for controlling CO2The flow rate of (c).
At present, no relevant report that the portable photosynthetic detector is used for detecting volatile organic compound gas of plant leaves exists.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information constitutes prior art already known to a person skilled in the art.
SUMMERY OF THE UTILITY MODEL
Purpose of the utility model
In order to solve the technical problem, an object of the present invention is to provide a volatile organic compound gas sampling device for plant leaves. The utility model provides a gaseous sampling device of plant leaf's volatile organic compounds has established the gaseous sampling device of VOCs of the plant leaf release that an environment is easily controlled, not disturbed, the gas circuit of high concentration VOCs in the air is reliable and stable.
Solution scheme
In order to realize the utility model discloses the purpose, the embodiment of the utility model provides a gaseous sampling device of volatile organic compound of plant leaf, include:
a portable photosynthetic analyzer, the portable photosynthetic analyzer comprising: the gas-liquid separation device comprises a control element, a working element, a vane chamber, a first gas path pipeline and a second gas path pipeline, wherein the first gas path pipeline is used for communicating a gas outlet of the control element with a gas inlet of the vane chamber;
the second gas path pipeline is also communicated with a third gas path pipeline, and a first switching valve, an adsorption pipe, a first flow controller and an air pump are sequentially arranged on the third gas path pipeline along the gas flowing direction.
In a possible implementation manner, the volatile organic compound gas sampling apparatus for plant leaves further includes:
a first air source for providing synthetic air;
and the first air source is communicated with an external air inlet on the control element through a fourth air path pipeline, and the fourth air path pipeline is also provided with a second flow controller.
In a possible implementation manner, the volatile organic compound gas sampling apparatus for plant leaves further includes:
the second gas source is used for providing standard gas; the standard gas is a gas with known component proportion;
and the second gas source is communicated with the first gas path pipeline through the fifth gas path pipeline, and the fifth gas path pipeline is also sequentially provided with a third flow controller and a second switching valve along the gas flowing direction.
In one possible implementation of the above device for sampling volatile organic compound from plant leaves, the first gas source is a 40 liter steel cylinder filled with synthetic air.
In a possible implementation manner, the second gas source is an 8-liter steel cylinder filled with standard gas.
In one possible implementation, the leaf chamber comprises a standard leaf chamber or a coniferous chamber suitable for broadleaf plant leaves: the standard leaf chamber is 2cm long and 4cm wide; the needle leaf chamber is 2cm long and 4cm wide.
In one possible implementation manner, the gas path pipeline is a Teflon transparent pipe with the outer diameter of 1/4 inches; the first switching valve and the second switching valve are stainless steel three-way valves.
In a possible implementation manner, the air pump is a small air sampling pump.
In one possible implementation of the volatile organic compound gas sampling device for plant leaves, the synthetic air consists of 21% of nitrogen and 79% of oxygen.
In one possible implementation manner, the standard gas is a volatile organic compound gas with a concentration of 10 × 10-6Volume percent isoprene.
Advantageous effects
(1) The embodiment of the utility model provides a gaseous sampling device of volatile organic compounds of plant leaf has established the gaseous sampling device of VOCs of the plant leaf release that an environment is easily controlled, the gas circuit is reliable and stable. Through portable photosynthetic apparatus, environmental conditions such as air temperature, illumination, carbon dioxide concentration of control leaf room that can be accurate, the test condition is controllable, and the test result is more accurate. The flow rate of the carrier gas can be stably controlled by a small-sized air sampling pump and a flow controller.
(2) The embodiment of the utility model provides a plant leaf's gaseous sampling device of volatile organic compound through first air supply, in open-circuit formula gas circulation system to synthetic air is the carrier gas, can get rid of the interference of high concentration VOCs in the air.
(3) The embodiment of the utility model provides a plant leaf's gaseous sampling device of volatile organic compound switches through the diverter valve, can introduce the VOCs composition gas of known concentration in the second air supply to can inspect entire system's reliability fast.
(4) The embodiment of the utility model provides a volatile organic compound gas sampling device of plant leaf, application scope is wide, can replace standard lobe of a leaf room for the needle leaf lobe room to accomplish the VOCS absorption that the plant needle leaf released.
Drawings
One or more embodiments are illustrated by the corresponding figures in the drawings, which are not meant to be limiting. The word "exemplary" is used exclusively herein to mean "serving as an example, embodiment, or illustration. Any embodiment described herein as "exemplary" is not necessarily to be construed as preferred or advantageous over other embodiments.
Fig. 1 is a schematic diagram of a volatile organic compound gas sampling device of a plant leaf according to an embodiment 1 of the present invention.
Fig. 2 is a schematic diagram of a sampling device for volatile organic compounds in plant leaves according to embodiment 1 of the present invention.
Fig. 3 is a schematic diagram of a sampling device for volatile organic compounds in plant leaves according to embodiment 1 of the present invention.
Description of the main reference numerals:
11-a control element, 12-a working element, 211-a first air path pipeline, 212-a second air path pipeline, 213-a third air path pipeline, 214-a fourth air path pipeline, 215-a fifth air path pipeline, 22-a first switching valve, 23-a second switching valve, 3-a first air source, 4-a second air source, 5-a vane chamber, 6-an adsorption tube, 7-an air pump, 81-a first flow controller, 82-a second flow controller and 83-a third flow controller.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention. Throughout the specification and claims, unless explicitly stated otherwise, the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element or component but not the exclusion of any other element or component.
Furthermore, in the following detailed description, numerous specific details are set forth in order to provide a better understanding of the present invention. It will be understood by those skilled in the art that the present invention may be practiced without some of these specific details. In some instances, materials, elements, methods, means, and the like that are well known to those skilled in the art have not been described in detail in order to avoid obscuring the present invention.
Example 1
As shown in fig. 1, a volatile organic compound gas sampling device for plant leaves comprises:
a portable photosynthetic analyzer, the portable photosynthetic analyzer comprising: the gas-liquid separation device comprises a control element 11, a working element 12, a vane chamber 5, a first gas path pipeline 211 for communicating a gas outlet of the control element with a gas inlet of the vane chamber, and a second gas path pipeline 212 for communicating the gas outlet of the vane chamber with the gas inlet of the control element;
the second air path pipeline 212 is further communicated with a third air path pipeline 213, and the third air path pipeline 213 is sequentially provided with a first switching valve 22, an adsorption tube 6, a first flow controller 81 and an air pump 7 along the air flowing direction.
As shown in fig. 2, in the above embodiment, optionally, the volatile organic compound gas sampling apparatus of the plant leaf further includes:
a first air source 3 for providing synthetic air;
and a fourth air path pipeline 214, wherein the first air source 3 is communicated with an external air inlet on the control element 11 through the fourth air path pipeline 214, and the fourth air path pipeline 214 is also provided with a second flow controller 82.
As shown in fig. 3, in the above embodiment, optionally, the volatile organic compound gas sampling apparatus of the plant leaf further includes:
a second gas source 4 for providing a standard gas; the standard gas is a gas with known component proportion;
and a fifth gas path pipeline 215, the second gas source 4 is communicated with the first gas path pipeline 211 through the fifth gas path pipeline 215; the fifth gas path pipeline 215 is sequentially provided with a second gas source 4, a third flow controller 83 and a second switching valve 23 along the gas flowing direction.
Optionally, in the above embodiment, the first gas source 3 is a 40 liter steel cylinder filled with synthetic air.
Optionally, in the above embodiment, the second gas source 4 is an 8 liter steel cylinder filled with standard gas.
Optionally, in the above embodiment, the leaf chamber 5 is a standard leaf chamber, and has a length of 2cm and a width of 4 cm; or the leaf chamber 5 is needle leaf chamber with length of 2cm and width of 4 cm.
Optionally in the above embodiment, the air passage conduit is a Teflon transparent tube with an outer diameter of 1/4 inches; the first switching valve 22 and the second switching valve 23 are stainless three-way valves.
Optionally, in the above embodiment, the air pump 7 is a small air sampling pump.
Optionally in the above embodiment, the synthetic air consists of 21% nitrogen and 79% oxygen.
Optionally, in the above embodiment, the standard gas has a concentration of 10 × 10-6Volume percent isoprene.
Example 2
As shown in fig. 1, a method for using a volatile organic compound gas sampling device of plant leaves comprises the following steps:
(1) firstly, selecting 3-5 plants with similar specifications as much as possible according to common garden plants, and selecting healthy and pest-free plants and leaves 3-6 th away from the top end of a branch as measurement objects on each plant;
(2) connecting the instrument as shown in FIG. 1;
(3) closing the first switching valve 22;
the leaves are clamped into a leaf chamber 5, and the environmental conditions of the leaf chamber 5 (i.e. the temperature of the leaves: 30 ℃; the photosynthetic effective radiation obtained by the leaves: 1000. mu. mol. m) are precisely controlled by a control element 11 and a working element 12-2·s-1(ii) a Air moisture content: 10000ppm by volume); CO that can also be carried by the control element 11 itself2Cylinders providing a stable volume percentage of 380ppm CO2Gas, which ensures the normal photosynthesis of plant leaves;
(4) after the portable photosynthetic apparatus runs for 10min, observing whether the numerical value of the host panel of the control element 11 reaches a set value;
(5) when the environmental condition of the leaf chamber 5 reaches a set value, the first switching valve 22 is opened, and 100 ml/min is supplied through the air pump 7 and the first flow controller 81-1The gas in the leaf chamber 5 containing the blades is pumped by the flow velocity and enters the adsorption tube 6 filled with the adsorbent through the third gas path pipeline 213 for adsorption and fixation; stopping the operation of the air pump 7 after 30min, taking out the adsorption tube 6, and sealing;
(6) the sealed adsorption tube 6 after adsorption is refrigerated (5 ℃), then taken out in sequence and analyzed for VOC composition and corresponding concentration in the adsorption tube 6 through a thermal desorption unit-gas chromatography-mass spectrometer.
Example 3
As shown in fig. 2, a method for using a volatile organic compound gas sampling device for plant leaves comprises the following steps:
(1) firstly, selecting 3-5 plants with similar specifications as much as possible according to common garden plants, and selecting healthy and pest-free plants and leaves 3-6 th away from the top end of a branch as measurement objects on each plant;
(2) connecting the instrument as shown in fig. 2;
(3) opening the first air source 3 and the second flow controller 82, and introducing the synthetic air in the first air source 3 into the air path system to be used as carrier air; closing the first switching valve 22;
the rest of the procedure was the same as in example 2.
Example 4
As shown in fig. 3, a method for using a volatile organic compound gas sampling device of plant leaves comprises the following steps:
(1) firstly, selecting 3-5 plants with similar specifications as much as possible according to common garden plants, and selecting healthy and pest-free plants and leaves 3-6 th away from the top end of a branch as measurement objects on each plant;
(2) connecting the instrument as shown in fig. 3;
(3) opening the first air source 3 and the second flow controller 82; the first switching valve 22 and the second switching valve 23 are closed;
the rest of the procedure was the same as in example 2.
When the reliability of the gas path needs to be checked, the second gas source 4, the third flow controller 83 and the second switching valve 23 can be opened, the standard gas in the second gas source 4 is introduced into the gas path through the fifth gas path pipeline 215 and is adsorbed, so as to verify the reliability of the whole gas path: at this time, the flow rate is controlled by the third flow controller 83 to 20ml min-1And the concentration is 10X 10-6The isoprene standard gas with volume percentage enters the gas path and is absorbed by the absorption tube 6; the adsorption test result of the isoprene standard gas is used for verifying the reliability of the whole gas circuit.
Finally, the biomass density and the adsorption analysis of the plant leaves are combined to obtain the specific discharge rate of the relevant components in the VOCs of the plant leaves (Table 1). The detection shows that the whole plant leaf volatile organic compound gas sampling device and the method meet the requirements.
TABLE 1 Standard emission rates of isoprene and monoterpenes from Beijing 22 species of landscape plant leaves (μ g C g (DW)-1h-1)
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.
Claims (10)
1. The utility model provides a gaseous sampling device of volatile organic compound of plant leaf which characterized in that: the volatile organic compound gas sampling device of the plant leaf comprises:
a portable photosynthetic analyzer, the portable photosynthetic analyzer comprising: the gas-liquid separation device comprises a control element, a working element, a vane chamber, a first gas path pipeline and a second gas path pipeline, wherein the first gas path pipeline is used for communicating a gas outlet of the control element with a gas inlet of the vane chamber;
the second gas path pipeline is also communicated with a third gas path pipeline, and a first switching valve, an adsorption pipe, a first flow controller and an air pump are sequentially arranged on the third gas path pipeline along the gas flowing direction.
2. The apparatus for sampling VOC in plant leaves as claimed in claim 1, wherein: the volatile organic compound gas sampling device of plant leaf still includes:
a first air source for providing synthetic air;
and the first air source is communicated with an external air inlet on the control element through a fourth air path pipeline, and the fourth air path pipeline is also provided with a second flow controller.
3. The apparatus for sampling VOC in plant leaves as claimed in claim 1, wherein: the volatile organic compound gas sampling device of plant leaf still includes:
the second gas source is used for providing standard gas; the standard gas is a gas with known component proportion;
and the second gas source is communicated with the first gas path pipeline through the fifth gas path pipeline, and the fifth gas path pipeline is also sequentially provided with a third flow controller and a second switching valve along the gas flowing direction.
4. The apparatus for sampling VOC in plant leaves as claimed in claim 1, wherein: the first source is a 40 liter cylinder containing synthetic air.
5. The apparatus for sampling VOC in plant leaves as claimed in claim 1, wherein: leaf chambers include standard leaf chambers or coniferous chambers suitable for broadleaf plant leaves: the standard leaf chamber is 2cm long and 4cm wide; the needle leaf chamber is 2cm long and 4cm wide.
6. The apparatus for sampling VOC in plant leaves as claimed in claim 3, wherein: the gas path pipeline is a Teflon transparent pipe with the outer diameter of 1/4 inches; the first switching valve and the second switching valve are stainless steel three-way valves.
7. The apparatus for sampling VOC in plant leaves as claimed in claim 1, wherein: the air pump is a small-sized air sampling pump.
8. The apparatus for sampling VOC in plant leaves as claimed in claim 2, wherein: synthetic air consists of 21% nitrogen and 79% oxygen.
9. The apparatus for sampling VOC in plant leaves as claimed in claim 3, wherein: the standard gas has a concentration of 10 × 10-6Volume percent isoprene.
10. The apparatus for sampling VOC in plant leaves as claimed in claim 1, wherein: the second gas source is an 8 liter steel cylinder filled with standard gas.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111879577A (en) * | 2020-08-11 | 2020-11-03 | 青岛理工大学 | Portable vegetation source volatile organic compound sampling system and working method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111879577A (en) * | 2020-08-11 | 2020-11-03 | 青岛理工大学 | Portable vegetation source volatile organic compound sampling system and working method thereof |
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