CN217059554U - Air bag for sampling plant volatile organic compounds in vivo - Google Patents
Air bag for sampling plant volatile organic compounds in vivo Download PDFInfo
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- CN217059554U CN217059554U CN202123400130.9U CN202123400130U CN217059554U CN 217059554 U CN217059554 U CN 217059554U CN 202123400130 U CN202123400130 U CN 202123400130U CN 217059554 U CN217059554 U CN 217059554U
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Abstract
An air bag for sampling plant volatile organic compounds in vivo belongs to the technical field of gas sampling. This air pocket comprises air pocket body and sealing clip, its characterized in that: the air bag body consists of a gas enrichment bag and an inflatable shaping bag fixedly connected with the outer side wall of the gas enrichment bag; the gas enrichment bag comprises a cylindrical first cavity, a circular truncated cone-shaped second cavity and an opening, and a gas outlet is formed in the circular bottom surface of the first cavity; and an inflation inlet is arranged on the side wall of the inflation shaping bag. The sampling device has the advantages of simple structure, ingenious design, convenience in carrying and operation, and is particularly suitable for sampling the volatile organic compounds of the field plants in vivo.
Description
Technical Field
The utility model belongs to the technical field of gaseous sampling, concretely relates to an air pocket for sampling of plant volatile organic compounds live body.
Background
Plant volatile organic compounds (BVOCs) are low-boiling point volatile small molecular organic compounds synthesized by plants through secondary metabolic pathways, and mainly comprise terpenes, hydrocarbons, aldehydes, alcohols, esters, ketones, acids and the like. Plant volatile organic compounds are mainly produced by leaves and released into the atmosphere, and have important influences on air quality, atmospheric environment, carbon circulation, human physical and psychological health and the like.
The factors influencing the release of the plant volatile organic compounds mainly include plant self factors and external environment factors, wherein: the plant factors comprise plant species, growth state, development stage and the like, and tree species difference is the primary factor determining the release of volatile organic compounds of trees, is the essential difference between trees, and plays an important role in the components and release rate of the volatile organic compounds of the plants. The method is used for determining the chemical components and release rate of volatile organic compounds released by different tree species under specific environmental conditions, which is the basis for developing volatile organic compound researches, so that the plant volatile organic compounds need to be sampled and measured.
The sampling method of the volatile organic compounds released by the plant leaves mainly comprises a static closed sampling method and a dynamic headspace sampling method, wherein the two methods are to lock branches, leaves or the whole plant of a certain outdoor tree species for sampling, and then bring a sampling adsorption tube back to a laboratory to measure the components and the content of the volatile organic compounds by using an analytical instrument. The static closed sampling method is to cover branch leaves of a tree part to be tested in an air bag, collect the air in the air bag at intervals after setting intervals, and measure the air by using an analytical instrument after collecting the air. In order to overcome the technical defect, in recent years, a dynamic headspace method is mainly adopted, which is also a method widely used at present for measuring volatile organic compounds emitted by living plants, and is different from a static closed sampling method in that two ends of an air bag used in the method are opened, the natural flow of air in the air bag is kept, air is sampled by a sampling pump, the air in the air bag is introduced into an adsorption tube or the air bag, and then computer detection and data analysis are carried out in a laboratory, wherein the release rate needs to be calculated according to parameters such as the change of the concentration of the air, the used time interval, the leaf area (or leaf weight) closed in the air bag, the volume of the air bag and the like, however, the air bag is made of a transparent soft material and has no fixed shape after being inflated, the volume of the air bag is closely related to the position of each tying, the flow rate of an air pump and the like, so that the volume of the air bag is difficult to be accurately calculated during sampling, accurate calculation of the release rate cannot be achieved. Based on the technical defect, the method for carrying out dynamic headspace sampling by using the air bag can only be used for qualitative and relative quantification, and absolute quantification is inaccurate. In order to be able to accurate ration, scientific research personnel try to adopt the glass container that has fixed volume to sample, but the glass container is heavy and breakable, carries inconveniently, needs longer equilibrium time moreover, consequently is not suitable for field plants live body sampling, can only be used for laboratory potted plant seedling sampling.
Disclosure of Invention
To the above-mentioned technical problem who exists in the background art, the utility model aims at designing an air pocket that not only is applicable to the laboratory but also is applicable to open-air plant volatile organic compounds live body sampling.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
an air bag for sampling the living plant volatile organic compounds, which consists of an air bag body and a sealing clip, and is characterized in that: the air bag body consists of a gas enrichment bag and an inflatable shaping bag fixedly connected with the outer side wall of the gas enrichment bag; the gas enrichment bag comprises a cylindrical first cavity, a circular truncated cone-shaped second cavity and an opening, and the circular bottom surface of the first cavity is provided with a gas outlet; and an inflation opening is formed in the side wall of the inflation shaping bag.
The air bag for sampling the plant volatile organic compounds in vivo is characterized in that: the sealing clamp comprises a first clamping strip provided with a groove and a second clamping strip provided with a convex strip, and a first through hole and a second through hole are further formed in the middle of the sealing clamp.
The air bag for sampling the plant volatile organic compounds in vivo is characterized in that: the cross section of the second holding strip is in an omega shape, and the second holding strip is matched with the first holding strip.
The air bag for sampling the plant volatile organic compounds in vivo is characterized in that: a first sealing washer consisting of two pairs of semi-circular rings is arranged on the wall of the first through hole; and a second sealing washer consisting of two pairs of semi-circular rings is arranged on the wall of the second through hole.
The air bag for sampling the plant volatile organic compounds in vivo is characterized in that: and one end of each of the first clamping strip and the second clamping strip is provided with a connecting piece.
The air bag for sampling the plant volatile organic compound living body is characterized in that: the gas enrichment bag is made of a light-transmitting soft material.
The utility model discloses an air pocket for sampling of plant volatile organic compounds living body, simple structure, design benefit, convenient to carry, the simple operation is particularly suitable for sampling of field plant volatile organic compounds living body. The beneficial effects are as follows: (1) the air bag is made of soft transparent materials, air in the air bag is emptied during transportation, and the air bag is foldable, light and handy and convenient to carry, so that the technical defects that materials such as glass are fragile and heavy are overcome. (2) The air bag body comprises gas enrichment bag and rather than the gas shaping bag of lateral wall fixed connection, during the use, is full of gas through the inflation inlet and aerifys the shaping bag, and along with aerifing going on, gas enrichment bag slowly swells, finally reaches full state completely, maintains its fixed shape to the realization can accurately calculate the volume of the interior air of air bag, is used for calculating the release rate of blade volatile organic compounds, realizes accurate ration. (3) The sealing clamp is composed of 2 clamping strips with special structures, the groove of the first clamping strip is matched with the convex strip of the second clamping strip, and the 2 pairs of through holes in the middle are used for accommodating branches and an air inlet pipeline respectively, so that the air bag can be fixed on the branches, and the branches can be sealed in the air bag.
Drawings
Fig. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the airbag body;
FIG. 3 is a schematic structural view of a first holding strip;
FIG. 4 is a schematic structural view of a second holding strip;
FIG. 5 is a schematic view of the semi-closed state of the enclosure clamp;
FIG. 6 is a schematic view of the closed state of the sealing clip;
FIG. 7 is a right side view of FIG. 6;
FIG. 8 is a schematic view of the structure of the present invention in use;
in the figure: 1-gas enrichment bag; 1-1-a first cavity; 1-2-a second cavity; 1-3-opening; 1-4-gas outlet; 2-inflating and shaping bags; 2-1-an inflation inlet; 3-sealing the clamp; 3-1-a first holding strip; 3-1A groove; 3-2-a second holding strip; 3-2B-convex strips; 3-3-a first via; 3-4-a second via; 3-3 s-first sealing gasket; 3-4 s-a second sealing gasket; 3-5-connectors; 4-branches; 5-an air inlet pipe, 5-1-a valve A; 6-a second absorption column; 7-an ozone removal column; 8-a first absorption column; 9-an air outlet pipe, 9-1-a valve B; 10-an adsorption tube; 11-a gas extraction pump.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings, which are not specifically described, but refer to the prior art.
The utility model discloses an air pocket (fig. 1) for sampling of plant volatile organic compounds living body, this air pocket includes air pocket body and seal clip 3, the air pocket body (fig. 2) includes inner bag and outer bag, the inner bag is for being used for holding branch and leaf and carrying out the gaseous enrichment bag 1 of enrichment with the gaseous state material of branch and leaf release, the outer bag is through aerifing in order to the design bag 2 of aerifing of setting for gaseous enrichment bag 1, the inner wall of gaseous enrichment bag 1 with aerify design bag 2 lateral walls and realize outer bag and inner bag fixed connection altogether. The gas enrichment bag 1 comprises a cylindrical first cavity 1-1, a truncated cone-shaped second cavity 1-2 and an opening 1-3, and the circular bottom surface of the first cavity is provided with a gas outlet 1-4; the side wall of the inflatable shaping bag 2 is provided with an inflation inlet 2-1.
In order to realize the sealing and fixing of the branches and leaves to be measured in the air bags, the invention designs a sealing clip 3 (figures 3-7), the sealing clip 3 comprises a first clip strip 3-1 and a second clip strip 3-2, wherein the longitudinal middle part of the first holding strip 3-1 is provided with a groove 3-1A, the longitudinal middle part of the second holding strip 3-2 is provided with a convex strip 3-2B which protrudes upwards, for better sealing and fastening, the ribs are arched, preferably in the range of 200-300, namely, the cross section of the second holding strip 3-2 is in an omega shape, the shape and the size of the convex strip are matched with those of the groove, the second holding strip 3-2 is embedded into the groove 3-1A of the first holding strip 3-1 through the convex strip 3-2B, the closing of the sealing clamp is realized, and the closed state is shown in figures 6 and 7. The middle part of the sealing clamp 3 is also provided with 2 pairs of through holes, namely a first through hole 3-3 and a second through hole 3-4, wherein one pair of through holes is used for accommodating branches and fixing the branches in the first through hole 3-3, the other pair of through holes is used as an air inlet, and air outside the air bag enters the gas enrichment bag 1 from the air inlet. In order to realize better sealing effect and not damage branches, a first sealing washer 3-3s consisting of two pairs of semi-rings is arranged on the wall of the first through hole 3-3; the hole wall of the second through hole 3-4 is provided with a second sealing washer 3-4s formed by two pairs of half rings, and it should be noted here that the middle part of the second holding strip 3-2 has no convex strip, that is, there is no convex strip between the first sealing washer and the second sealing washer on both sides of the convex strip (see fig. 4).
Preferably, one end of the first holding strip 3-1 and the second holding strip 3-2 is preferably provided with a connecting member 3-5, so that the alignment and matching of the first holding strip 3-1 and the second holding strip 3-2 can be better achieved.
Since most plant volatile substances are released depending on light, the air bag for collecting plant volatile organic compounds needs to be made of a light-transmitting soft material, and the material of the air bag for enriching the gas needs to release no volatile organic compounds and absorb little or no volatile organic compounds released by plants, such as polytetrafluoroethylene material and the like.
When the air bag is used for collecting plant volatile organic compounds by a dynamic headspace sampling method (see figure 8), the operation steps are as follows:
1) preparing the air bag, namely the air bag for sampling the living plant volatile organic compounds;
2) inflating the inflatable shaping bag 2 through the inflation inlet 2-1 to 1/3-2/3 in a saturated state;
3) selecting a branch 4 to be tested, and carefully inserting the branch into the gas enrichment bag 1 from the opening 1-3;
4) aligning the branches with the second through hole, wherein the first through hole is used for placing an air inlet pipe 5, the sealing clamp 3 is used for clamping the opening 1-3, a valve A5-1 is arranged at one end, close to the air bag, of the air inlet pipe 5, and the valve A is adjusted to be in an off state; in order to make the air entering the gas enrichment bag be clean air, the other end of the air inlet pipe 5 is sequentially connected with a second absorption column 6 (for absorbing volatile organic compounds in the air), an ozone removal column 7 (for removing ozone) and a first absorption column 8 (for absorbing water vapor and part of volatile organic compounds);
5) connecting an air outlet pipe 9 with an air outlet 1-4, wherein one end of the air outlet pipe 9 close to the air outlet 1-4 is provided with a valve B9-1, and the valve B is adjusted to be in an 'on' state;
6) opening the inflation inlet to discharge the gas in the inflatable shaping bag 2;
7) slightly pressing the air bag to discharge residual air in the gas enrichment bag 1 from the air outlet 1-4;
8) adjusting the valve A to be in an 'on' state, and adjusting the valve B to be in an 'off' state;
9) slowly inflating the inflatable shaping bag 2 from the inflating port 2-1 to a saturated state, and then sucking clean air from the air inlet by the gas enrichment bag 1;
10) the outlet end of the gas outlet pipe 9 is connected with a gas production pump 11, the valve B is adjusted to be in an 'on' state, and the gas production pump 11 is started until the gas in the gas enrichment bag 1 reaches a balanced state; then, an adsorption pipe 10 is connected to the air inlet end of the gas production pump 11, and the gas production pump 11 is started to sample the plant volatile organic compounds; at the moment, the volume of the gas enrichment bag 1 is equal to the sum of the volume of the cylindrical first cavity 1-1 and the volume of the truncated cone-shaped second cavity 1-2.
The air bag designed by the invention has the following beneficial effects: (1) the air bag is made of soft transparent materials, air in the air bag is emptied during transportation, and the air bag is foldable, light and handy and convenient to carry, so that the technical defects that materials such as glass are fragile and heavy are overcome. (2) The air bag body comprises gas enrichment bag and rather than the inflation design bag of lateral wall fixed connection, during the use, is full of gas through the inflation inlet and aerifys the design bag, and along with aerifing going on, gas enrichment bag slowly swells, finally reaches full state, maintains its fixed shape to the realization can accurately calculate the volume of air in the air bag for calculate the release rate of blade volatility organic matter, realize accurate ration. (3) The sealing clamp is composed of 2 clamping strips with special structures, the groove of the first clamping strip is matched with the convex strip of the second clamping strip, and the 2 pairs of through holes in the middle are used for accommodating branches and air inlet pipelines respectively, so that the air bag can be fixed on the branches, and the branches can be sealed in the air bag.
Claims (6)
1. An air bag for sampling the living body of plant volatile organic compounds, which consists of an air bag body and a sealing clamp (3), and is characterized in that: the air bag body consists of a gas enrichment bag (1) and an inflatable shaping bag (2) fixedly connected with the outer side wall of the gas enrichment bag; the gas enrichment bag (1) comprises a cylindrical first cavity (1-1), a truncated cone-shaped second cavity (1-2) and an opening (1-3), and a gas outlet (1-4) is formed in the circular bottom surface of the first cavity; the side wall of the inflatable shaping bag (2) is provided with an inflation inlet (2-1).
2. The air bag for sampling the living body of the plant volatile organic compounds according to claim 1, characterized in that: the sealing clamp (3) comprises a first clamping strip (3-1) provided with a groove (3-1A) and a second clamping strip (3-2) provided with a convex strip (3-2B), and a first through hole (3-3) and a second through hole (3-4) are further formed in the middle of the sealing clamp.
3. An air bag for in vivo sampling of plant volatile organic compounds as claimed in claim 2, wherein: the cross section of the second holding strip (3-2) is in an omega shape, and the second holding strip is matched with the first holding strip (3-1).
4. The air bag for sampling living plant volatile organic compounds according to claim 2, wherein: a first sealing washer (3-3 s) consisting of two pairs of semi-circular rings is arranged on the wall of the first through hole (3-3); and a second sealing washer (3-4 s) consisting of two pairs of semi-circular rings is arranged on the wall of the second through hole (3-4).
5. The air bag for sampling living plant volatile organic compounds according to claim 2, wherein: one end of the first holding strip (3-1) and one end of the second holding strip (3-2) are provided with connecting pieces (3-5).
6. The air bag for sampling living plant volatile organic compounds according to claim 1, wherein: the gas enrichment bag (1) is made of a light-transmitting soft material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123400130.9U CN217059554U (en) | 2021-12-31 | 2021-12-31 | Air bag for sampling plant volatile organic compounds in vivo |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123400130.9U CN217059554U (en) | 2021-12-31 | 2021-12-31 | Air bag for sampling plant volatile organic compounds in vivo |
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| Publication Number | Publication Date |
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| CN217059554U true CN217059554U (en) | 2022-07-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202123400130.9U Active CN217059554U (en) | 2021-12-31 | 2021-12-31 | Air bag for sampling plant volatile organic compounds in vivo |
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2021
- 2021-12-31 CN CN202123400130.9U patent/CN217059554U/en active Active
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