CN114152605A - Method for detecting content of amino acid in feed - Google Patents
Method for detecting content of amino acid in feed Download PDFInfo
- Publication number
- CN114152605A CN114152605A CN202111394459.0A CN202111394459A CN114152605A CN 114152605 A CN114152605 A CN 114152605A CN 202111394459 A CN202111394459 A CN 202111394459A CN 114152605 A CN114152605 A CN 114152605A
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- feed
- content
- perchloric acid
- amino acid
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/16—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using titration
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention provides a method for detecting the content of amino acid in feed, which comprises the following steps: (1) weighing 1g of dried feed sample, precisely weighing, (2) adding acetic acid, stirring until the feed sample is completely dissolved, (3) adding a crystal violet indicator, (4) titrating a liquid drop by perchloric acid until the solution is blue-green, namely an end point, (5) recording the volume of consumed perchloric acid titrating liquid as Vs, simultaneously performing a blank test, recording the volume of the consumed perchloric acid titrating liquid as Vo, and (6) calculating the content of amino acid in the feed sample according to a formula. By adopting a perchloric acid titration method, the interference of substances such as sucrose, starch and the like in the feed can be effectively eliminated, and the content of amino acid in the feed can be accurately and conveniently measured.
Description
Technical Field
The invention relates to the field of detection of amino acid content, in particular to a method for detecting the content of amino acid in feed.
Background
Amino acid additives are nutritive substances required in feed to balance or complement a particular production purpose. Amino acids are the basic units that make up proteins. The essence of protein nutrition is amino acid nutrition. The core of amino acid nutrition is the balance between amino acids. The amino acid balance of natural feed is very poor and almost unbalanced, the amino acid content of natural feed is very different and different, and the full-value compound feed formed by different types and proportions of natural feed still has various amino acid contents and the proportion of amino acid which are changed and varied according to the principle of amino acid balance. Thus, amino acid additives are needed to balance or complement the required amount required for a particular production purpose.
Currently, the common methods for determining amino acids mainly include: chemical analysis, spectrophotometry, mass spectrometry, and the like. Although these methods have the advantages of many kinds of amino acids to be measured, high sensitivity, high accuracy and the like, these methods have the problems of complicated sample treatment, destructive sampling, low throughput, high requirements on instruments, high price and the like.
Disclosure of Invention
In order to solve the defects of the prior art, the method for detecting the content of the amino acid in the feed comprises the following steps:
s1: weighing 1g of dried feed sample, and precisely weighing;
s2, adding acetic acid, and stirring until the acetic acid is completely dissolved;
s3: adding a crystal violet indicator;
s4: titrating the liquid drop test solution by perchloric acid until the solution is blue-green, namely the end point;
and S5, recording the volume of the consumed perchloric acid titration solution as Vs, simultaneously performing a blank test, and recording the volume of the blank consumed perchloric acid titration solution as Vo.
S6: and calculating the content of the amino acid in the feed sample.
Preferably, the concentration of the perchloric acid titration solution is 1 mol/L.
Preferably, the amount of acetic acid added in S2 is 50 ml.
Preferably, the crystal violet indicator is added in an amount of two drops in S3.
Preferably, the formula for calculating the content of the amino acid in S6 is as follows:
wherein m is the sample weight;
vs is the volume of perchloric acid titration solution consumed by the sample;
v0 is the volume of perchloric acid titration solution consumed in the blank;
c is the actually measured concentration of perchloric acid titration solution;
0.85 is 1ml of perchloric acid titration solution corresponding to the mass number of amino acids.
Has the advantages that:
(1) the method is different from the traditional methods for determining the amino acid, namely a chemical analysis method, a spectrophotometry method, a mass spectrometry method and the like, and has the problems of complex sample treatment, destructive sampling, low flux, high requirement on instruments, high price and the like.
(2) By adopting a perchloric acid titration method, the interference of substances such as sucrose, starch and the like in the feed can be effectively eliminated, and the content of amino acid in the feed can be accurately and conveniently measured.
Detailed Description
For the purpose of enhancing understanding of the present invention, the present invention will be further described in detail with reference to the following examples, which are provided for illustration only and are not to be construed as limiting the scope of the present invention.
Example (b):
weighing 1g of dried feed sample, precisely weighing, adding 50ml of acetic acid, stirring until the acetic acid is completely dissolved, adding two drops of crystal violet indicator, titrating the test solution by using perchloric acid with the concentration of 1mol/L until the solution is blue-green, namely the end point, recording the volume of consumed perchloric acid titrating solution as Vs, simultaneously performing a blank test, recording the volume of blank consumed perchloric acid titrating solution as Vo, and calculating the content of amino acid in the feed sample according to a formula.
The invention provides a method for detecting amino acid by using perchloric acid titration, which does not need special instruments, has strong operability and is simple and easy to implement. By adopting a perchloric acid titration method, the interference of substances such as sucrose, starch and the like in the feed can be effectively eliminated, and the content of amino acid in the feed can be accurately and conveniently measured.
As a further improvement, the above-mentioned is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. A method for detecting the content of amino acid in feed is characterized by comprising the following steps:
s1: weighing 1g of dried feed sample, and precisely weighing;
s2, adding acetic acid, and stirring until the acetic acid is completely dissolved;
s3: adding a crystal violet indicator;
s4: titrating the liquid drop test solution by perchloric acid until the solution is blue-green, namely the end point;
and S5, recording the volume of the consumed perchloric acid titration solution as Vs, simultaneously performing a blank test, and recording the volume of the blank consumed perchloric acid titration solution as Vo.
S6: and calculating the content of the amino acid in the feed sample according to a formula.
2. The method for detecting the content of amino acids in feed according to claim 1, wherein the concentration of the perchloric acid titration solution is 1 mol/L.
3. The method for detecting the content of amino acids in feed according to claim 1, wherein the amount of acetic acid added in S2 is 50 ml.
4. The method according to claim 1, wherein the crystal violet indicator is added in an amount of two drops in S3.
5. The method for detecting the content of amino acids in feed according to claim 1, wherein the formula for calculating the content of amino acids in S6 is as follows:
wherein m is the sample weight;
vs is the volume of perchloric acid titration solution consumed by the sample;
v0 is the volume of perchloric acid titration solution consumed in the blank;
c is the actually measured concentration of perchloric acid titration solution;
0.85 is 1ml of perchloric acid titration solution corresponding to the mass number of amino acids.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111394459.0A CN114152605A (en) | 2021-11-23 | 2021-11-23 | Method for detecting content of amino acid in feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111394459.0A CN114152605A (en) | 2021-11-23 | 2021-11-23 | Method for detecting content of amino acid in feed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114152605A true CN114152605A (en) | 2022-03-08 |
Family
ID=80457105
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111394459.0A Pending CN114152605A (en) | 2021-11-23 | 2021-11-23 | Method for detecting content of amino acid in feed |
Country Status (1)
| Country | Link |
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| CN (1) | CN114152605A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007232581A (en) * | 2006-03-01 | 2007-09-13 | Tokuyama Corp | Quantitative determination method of amino acids contained in amino acids-zinc complex |
| CN103336046A (en) * | 2013-04-17 | 2013-10-02 | 中国医药研究开发中心有限公司 | Retigabine compound content determination method |
| CN103529032A (en) * | 2012-07-06 | 2014-01-22 | 贵州百灵企业集团制药股份有限公司 | Detection method for measuring content of N-methylmorpholine |
| CN104198479A (en) * | 2014-08-16 | 2014-12-10 | 中山鼎晟生物科技有限公司 | Rapid detection kit for sodium glutamate in gourmet powder |
-
2021
- 2021-11-23 CN CN202111394459.0A patent/CN114152605A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007232581A (en) * | 2006-03-01 | 2007-09-13 | Tokuyama Corp | Quantitative determination method of amino acids contained in amino acids-zinc complex |
| CN103529032A (en) * | 2012-07-06 | 2014-01-22 | 贵州百灵企业集团制药股份有限公司 | Detection method for measuring content of N-methylmorpholine |
| CN103336046A (en) * | 2013-04-17 | 2013-10-02 | 中国医药研究开发中心有限公司 | Retigabine compound content determination method |
| CN104198479A (en) * | 2014-08-16 | 2014-12-10 | 中山鼎晟生物科技有限公司 | Rapid detection kit for sodium glutamate in gourmet powder |
Non-Patent Citations (2)
| Title |
|---|
| 贾铮;李兰;赵根龙;肖志明;王石;李守学;樊霞;: "饲料中氨基酸分析技术研究进展", 农产品质量与安全, no. 06, pages 53 - 60 * |
| 齐立珠等: "进口饲料添加剂氨基酸类纯度的测定", 《现代商检科技》, vol. 3, no. 5, pages 57 - 60 * |
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