CN114144529A - 用于基因分型的试剂盒 - Google Patents

用于基因分型的试剂盒 Download PDF

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Publication number
CN114144529A
CN114144529A CN202180004121.1A CN202180004121A CN114144529A CN 114144529 A CN114144529 A CN 114144529A CN 202180004121 A CN202180004121 A CN 202180004121A CN 114144529 A CN114144529 A CN 114144529A
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China
Prior art keywords
probe
genotyping
primer
template
sequence
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Pending
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CN202180004121.1A
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English (en)
Chinese (zh)
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A·斯莱特
E·H·维尔马斯
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Inmair Cambridge Ltd
Inmair Ltd
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Inmair Cambridge Ltd
Inmair Ltd
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Priority claimed from PCT/US2021/019410 external-priority patent/WO2021173666A1/en
Publication of CN114144529A publication Critical patent/CN114144529A/zh
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/313—Type II endonucleases, i.e. cutting outside recognition site
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/113—Modifications characterised by incorporating modified backbone
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/131—Modifications characterised by incorporating a restriction site
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/518—Detection characterised by immobilisation to a surface characterised by the immobilisation of the nucleic acid sample or target
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/543—Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification

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  • Chemical & Material Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202180004121.1A 2020-02-26 2021-02-24 用于基因分型的试剂盒 Pending CN114144529A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202062981866P 2020-02-26 2020-02-26
US62/981866 2020-02-26
PCT/US2021/019410 WO2021173666A1 (en) 2020-02-26 2021-02-24 Kits for genotyping

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Publication Number Publication Date
CN114144529A true CN114144529A (zh) 2022-03-04

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CN202180004121.1A Pending CN114144529A (zh) 2020-02-26 2021-02-24 用于基因分型的试剂盒

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US (1) US20210262028A1 (da)
EP (2) EP3969613B1 (da)
JP (1) JP2023514887A (da)
KR (1) KR20220145252A (da)
CN (1) CN114144529A (da)
AU (1) AU2021227206A1 (da)
BR (1) BR112021026795A2 (da)
CA (1) CA3145539A1 (da)
DK (1) DK3969613T3 (da)
ES (1) ES2960746T3 (da)
FI (1) FI3969613T3 (da)
IL (1) IL289118A (da)
MX (1) MX2021015806A (da)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024041749A1 (en) * 2022-08-23 2024-02-29 Leica Microsystems Cms Gmbh Sequencing scaffold and method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103228798A (zh) * 2010-09-24 2013-07-31 斯坦福大学托管董事会 使用固定的引物直接捕获、扩增及测序靶标dna
WO2016162309A1 (en) * 2015-04-10 2016-10-13 Spatial Transcriptomics Ab Spatially distinguished, multiplex nucleic acid analysis of biological specimens
CN107208019A (zh) * 2014-11-11 2017-09-26 伊鲁米纳剑桥有限公司 用于核酸单克隆簇的产生和测序的方法和阵列
WO2018161019A1 (en) * 2017-03-03 2018-09-07 Counsyl, Inc. Methods for optimizing direct targeted sequencing
CN110770354A (zh) * 2017-04-19 2020-02-07 鹍远基因公司 用于文库构建和序列分析的组合物和方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10036013B2 (en) * 2013-08-19 2018-07-31 Abbott Molecular Inc. Next-generation sequencing libraries
CA2950295C (en) * 2014-06-06 2023-10-17 Cornell University Method for identification and enumeration of nucleic acid sequence, expression, copy, or dna methylation changes, using combined nuclease, ligase, polymerase, and sequencing reactions

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103228798A (zh) * 2010-09-24 2013-07-31 斯坦福大学托管董事会 使用固定的引物直接捕获、扩增及测序靶标dna
CN107208019A (zh) * 2014-11-11 2017-09-26 伊鲁米纳剑桥有限公司 用于核酸单克隆簇的产生和测序的方法和阵列
WO2016162309A1 (en) * 2015-04-10 2016-10-13 Spatial Transcriptomics Ab Spatially distinguished, multiplex nucleic acid analysis of biological specimens
WO2018161019A1 (en) * 2017-03-03 2018-09-07 Counsyl, Inc. Methods for optimizing direct targeted sequencing
CN110770354A (zh) * 2017-04-19 2020-02-07 鹍远基因公司 用于文库构建和序列分析的组合物和方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ERIK S. HOPMANS ET AL.: "A programmable method for massively parallel targeted sequencing", 《NUCLEIC ACIDS RESEARCH》, vol. 42, no. 10, pages 1 - 16 *
SAMUEL MYLLYKANGAS ET AL.: "E fficient targeted resequencing of human germline and cancer genomes by oligonucleotide-selective sequencing", 《NATURE BIOTECHNOLOGY》, vol. 29, no. 11, pages 1024 - 1029 *

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Publication number Publication date
US20210262028A1 (en) 2021-08-26
DK3969613T3 (da) 2024-01-08
ES2960746T3 (es) 2024-03-06
KR20220145252A (ko) 2022-10-28
CA3145539A1 (en) 2021-09-02
IL289118A (en) 2022-02-01
AU2021227206A1 (en) 2022-01-27
JP2023514887A (ja) 2023-04-12
EP3969613B1 (en) 2023-10-04
BR112021026795A2 (pt) 2022-02-15
EP4253559A3 (en) 2023-10-25
MX2021015806A (es) 2022-04-27
EP4253559A2 (en) 2023-10-04
FI3969613T3 (fi) 2023-10-06
EP3969613A1 (en) 2022-03-23

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