CN114127127A - 嵌合的人乳头瘤病毒35型l1蛋白 - Google Patents

嵌合的人乳头瘤病毒35型l1蛋白 Download PDF

Info

Publication number
CN114127127A
CN114127127A CN202080052245.2A CN202080052245A CN114127127A CN 114127127 A CN114127127 A CN 114127127A CN 202080052245 A CN202080052245 A CN 202080052245A CN 114127127 A CN114127127 A CN 114127127A
Authority
CN
China
Prior art keywords
type
protein
hpv
fragment
chimeric
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202080052245.2A
Other languages
English (en)
Other versions
CN114127127B (zh
Inventor
谢良志
罗春霞
张伟
索晓燕
庞琳
胡萍
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sinocelltech Ltd
Original Assignee
Sinocelltech Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sinocelltech Ltd filed Critical Sinocelltech Ltd
Publication of CN114127127A publication Critical patent/CN114127127A/zh
Application granted granted Critical
Publication of CN114127127B publication Critical patent/CN114127127B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01—DNA viruses
    • C07K14/025—Papovaviridae, e.g. papillomavirus, polyomavirus, SV40, BK virus, JC virus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/04—Inactivation or attenuation; Producing viral sub-units

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

本发明提供了嵌合的人乳头瘤病毒35型L1蛋白和编码其的多核苷酸，还提供了HPV 35型病毒样颗粒和其制备方法。该嵌合的人乳头瘤病毒35型L1蛋白包含衍生于HPV 35型L1蛋白的N端片段，该N端片段保持HPV 35型L1蛋白的免疫原性；和衍生于第二型别乳头瘤病毒L1蛋白的C端片段，该第二型别乳头状瘤病毒L1蛋白具有相较于其他型别的L1蛋白表达量和可溶性较好的特性；其中该嵌合的HPV 35型L1蛋白具有HPV 35型L1蛋白的免疫原性。该嵌合的人乳头瘤病毒35型L1蛋白具有较高的表达量和可溶性，可用于疫苗的大规模生产。

Description

PCT国内申请，说明书已公开。

Claims (23)

  1. PCT国内申请，权利要求书已公开。
CN202080052245.2A 2019-07-19 2020-07-17 嵌合的人乳头瘤病毒35型l1蛋白 Active CN114127127B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2019106563154 2019-07-19
CN201910656315 2019-07-19
PCT/CN2020/102599 WO2021013070A1 (zh) 2019-07-19 2020-07-17 嵌合的人乳头瘤病毒35型l1蛋白

Publications (2)

Publication Number Publication Date
CN114127127A true CN114127127A (zh) 2022-03-01
CN114127127B CN114127127B (zh) 2024-04-09

Family

ID=74193230

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080052245.2A Active CN114127127B (zh) 2019-07-19 2020-07-17 嵌合的人乳头瘤病毒35型l1蛋白

Country Status (2)

Country Link
CN (1) CN114127127B (zh)
WO (1) WO2021013070A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024140161A1 (zh) * 2022-12-28 2024-07-04 北京康乐卫士生物技术股份有限公司 人乳头瘤病毒hpv35 l1蛋白的表达和类病毒样颗粒及其制备方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117083078A (zh) * 2021-01-14 2023-11-17 神州细胞工程有限公司 一种人乳头瘤病毒病毒样颗粒疫苗的稳定制剂

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001097840A1 (en) * 2000-06-21 2001-12-27 Medimmune, Inc. Chimeric human papillomavirus (hpv) l1 molecules and uses therefor
CN106399329A (zh) * 2015-08-12 2017-02-15 北京康乐卫士生物技术股份有限公司 33型重组人乳头瘤病毒病毒样颗粒及其制备方法
CN106831959A (zh) * 2015-12-04 2017-06-13 厦门大学 一种人乳头瘤病毒33型l1蛋白的突变体
CN107188966A (zh) * 2016-03-15 2017-09-22 中国医学科学院基础医学研究所 一种乳头瘤病毒嵌合蛋白及其用途
CN109251236A (zh) * 2017-07-14 2019-01-22 厦门大学 一种人乳头瘤病毒35型l1蛋白的突变体

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001097840A1 (en) * 2000-06-21 2001-12-27 Medimmune, Inc. Chimeric human papillomavirus (hpv) l1 molecules and uses therefor
CN106399329A (zh) * 2015-08-12 2017-02-15 北京康乐卫士生物技术股份有限公司 33型重组人乳头瘤病毒病毒样颗粒及其制备方法
CN106831959A (zh) * 2015-12-04 2017-06-13 厦门大学 一种人乳头瘤病毒33型l1蛋白的突变体
CN107188966A (zh) * 2016-03-15 2017-09-22 中国医学科学院基础医学研究所 一种乳头瘤病毒嵌合蛋白及其用途
CN109251236A (zh) * 2017-07-14 2019-01-22 厦门大学 一种人乳头瘤病毒35型l1蛋白的突变体

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DONG PAN等: "Enhanced expression of soluble human papillomavirus L1 through coexpression of molecular chaperonin in Escherichia coli", 《 PROTEIN EXPR PURIF》, vol. 120, pages 92 - 98, XP029393669, DOI: 10.1016/j.pep.2015.12.016 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024140161A1 (zh) * 2022-12-28 2024-07-04 北京康乐卫士生物技术股份有限公司 人乳头瘤病毒hpv35 l1蛋白的表达和类病毒样颗粒及其制备方法

Also Published As

Publication number Publication date
CN114127127B (zh) 2024-04-09
WO2021013070A1 (zh) 2021-01-28

Similar Documents

Publication Publication Date Title
CN114127098B (zh) 嵌合的人乳头瘤病毒51型l1蛋白
CN114364692B (zh) 嵌合的乳头瘤病毒l1蛋白
CN114127092B (zh) 人乳头瘤病毒多价免疫原性组合物
CN114127100B (zh) 嵌合的人乳头瘤病毒39型l1蛋白
CN114127127B (zh) 嵌合的人乳头瘤病毒35型l1蛋白
CN114127097B (zh) 嵌合的人乳头瘤病毒56型l1蛋白
CN114174319B (zh) 嵌合的人乳头瘤病毒52型l1蛋白
CN114127096B (zh) 嵌合的人乳头瘤病毒31型l1蛋白
CN114127095B (zh) 嵌合的人乳头瘤病毒11型l1蛋白
CN114127093B (zh) 嵌合的人乳头瘤病毒45型l1蛋白
CN114127094B (zh) 嵌合的人乳头瘤病毒58型l1蛋白
CN114127295B (zh) 嵌合的人乳头瘤病毒16型l1蛋白
CN114174320B (zh) 嵌合的人乳头瘤病毒18型l1蛋白
CN114127099B (zh) 嵌合的人乳头瘤病毒6型l1蛋白
BR112022001075B1 (pt) Proteína l1 quimérica de papilomavírus, partícula similar ao papilomavírus (vlp- virus like particle), composição imunogênica para prevenção de doenças ou infecções associadas ao papilomavírus, polinucleotídeo isolado, vetor, baculovírus e método in vitro para preparar uma partícula similar ao papilomavírus

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40066223

Country of ref document: HK

GR01 Patent grant
GR01 Patent grant