CN114112043B - Spectrum imaging device - Google Patents
Spectrum imaging device Download PDFInfo
- Publication number
- CN114112043B CN114112043B CN202111340651.1A CN202111340651A CN114112043B CN 114112043 B CN114112043 B CN 114112043B CN 202111340651 A CN202111340651 A CN 202111340651A CN 114112043 B CN114112043 B CN 114112043B
- Authority
- CN
- China
- Prior art keywords
- spectrum
- cylindrical lens
- scene
- lens
- array
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000001228 spectrum Methods 0.000 title claims abstract description 54
- 238000003384 imaging method Methods 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 15
- 230000003595 spectral effect Effects 0.000 claims abstract description 6
- 238000000701 chemical imaging Methods 0.000 claims description 5
- 239000006185 dispersion Substances 0.000 claims description 4
- 230000003321 amplification Effects 0.000 claims description 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 2
- 239000000758 substrate Substances 0.000 claims description 2
- 230000003287 optical effect Effects 0.000 claims 1
- 239000012472 biological sample Substances 0.000 abstract description 3
- 238000005192 partition Methods 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/40—Measuring the intensity of spectral lines by determining density of a photograph of the spectrum; Spectrography
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- General Physics & Mathematics (AREA)
- Spectrometry And Color Measurement (AREA)
Abstract
The invention discloses a spectrum imaging device which comprises a lens, a blazed grating, a band-pass filter, an achromatic relay lens group, a cylindrical lens array and a camera, wherein the lens, the blazed grating, the band-pass filter and the achromatic relay lens group are sequentially arranged, and each cylindrical lens in the camera corresponds to a group of sub-pixel strips. According to the invention, through a unique light path design, three-dimensional spectrum data is recorded in a two-dimensional pixel space through pixel partition multiplexing, at the moment, the transverse resolution and the spectrum resolution of single-channel imaging are in an inverse proportion relationship, and meanwhile, the longitudinal resolution of single-channel imaging has no influence, so that single exposure acquisition of a three-dimensional spectrum image can be realized, and a pixel area is fully utilized; the device can acquire scene spectrum data in real time and display the scene spectrum data in real time, and the time delay is avoided in the data acquisition and data processing process; spectral data of a dynamic scene can be obtained, and the brightness of a light source is moderate, so that the biological sample is not damaged by photobleaching and the like.
Description
Technical Field
The invention relates to the field of spectrum imaging, in particular to a spectrum imaging device.
Technical Field
Compared with the traditional imaging technology, the spectrum imaging can record the spectrum information of the two-dimensional image of the scene while shooting the two-dimensional image of the scene, and the two-dimensional space and the one-dimensional spectrum information are recorded. The spectral imaging technology can increase the richness of recorded information and is beneficial to later analysis and processing. In the early stage of the spectrum imaging technology, the traditional method is used for acquiring spectrum information, namely, two-dimensional space information and spectrum information at corresponding wavelengths are recorded through a narrow-band filter. The method has the advantages of high precision and easiness in implementation, and has the defects that only a limited plurality of spectrum channel information can be acquired and the spectrum information is incoherent. Meanwhile, the method cannot record the spectrum information on different spectrum channels at the same time, so that only the spectrum imaging of a static scene can be realized.
The spectrum imaging device can acquire a plurality of spectrum channels, so that spectrum data is richer, and meanwhile, the pixel array space is not sacrificed, so that the utilization rate of the pixel space is higher. Therefore, the spectrum imaging device can effectively solve the problems that the spectrum channels are few and the dynamic scene image acquisition cannot be processed in the early spectrum imaging technology.
The invention comprises the following steps:
aiming at the defects in the prior art, the invention provides a spectrum imaging device. The device can record two-dimensional space information and one-dimensional spectrum information simultaneously, and can be used for recording the spectrum microscopic imaging of a dynamic scene.
A spectrum imaging device comprises a lens (1), a blazed grating (2), a band-pass filter (3), an achromatic relay lens group (4), a cylindrical lens array (5) and a camera (6), wherein each cylindrical lens in the camera corresponds to a group of sub-pixel strips (7).
Under natural light, scene images observed by the lens (1) are converged on a back focal plane of the lens (1), a blazed grating (2) is arranged at the back focal plane, the blazed grating (2) disperses the scene images recorded by the lens (1) and relays the scene images to one side of a substrate of the cylindrical lens array (5) through the achromatic relay lens group (4), at the moment, the scene images at the blazed grating (2) and the cylindrical lens array (5) are not dispersed, and the scene images are dispersed in the process of being transmitted to the cylindrical lens array (5) from the blazed grating (2). The pixel array of the camera (6) is located at the back focal plane of the lenticular lens array (5), and light signals with different wavelengths of scene images converged on the lenticular lens array (5) have different emergence angles, so that the light signals with different wavelengths of the scene images passing through the lenticular lens array (5) can be dispersed along the normal direction of the lenticular lens and spread over the pixel array of the camera (6), and the tangential scene images of the lenticular lens are not dispersed.
The invention has the following beneficial effects:
1. according to the invention, through a unique light path design, three-dimensional spectrum data is recorded in a two-dimensional pixel space through pixel partition multiplexing, at the moment, the transverse resolution and the spectrum resolution of single-channel imaging are in an inverse proportion relationship, and meanwhile, the longitudinal resolution of single-channel imaging has no influence, so that single exposure acquisition of a three-dimensional spectrum image can be realized, and a pixel area is fully utilized;
2. the device can acquire scene spectrum data in real time and display the scene spectrum data in real time, and the time delay is avoided in the data acquisition and data processing process;
3. the device can obtain the spectrum data of the dynamic scene, has moderate light source brightness, and can not cause damage such as photobleaching and the like to biological samples.
Drawings
FIG. 1 is a block diagram of a spectral imaging apparatus according to an embodiment of the present invention;
the system comprises a 1-lens, a 2-blazed grating, a 3-band-pass filter, a 4-achromatic relay lens group, a 5-cylindrical lens array, a 6-camera and 7-sub-pixel strips.
Fig. 2 is a schematic diagram of initial imaging data of a spectral imaging device according to an embodiment of the invention.
Detailed Description
The invention provides a spectrum imaging device, which is characterized in that a single exposure acquires a plurality of continuous spectrum information of a biological sample, and the method comprises the following steps:
referring to fig. 1, the spectral imaging apparatus according to the embodiment of the present invention includes a lens 1, a blazed grating 2, a band pass filter 3, an achromatic relay lens group 4, a lenticular lens array 5, a camera 6, and a subpixel bar 7, which are sequentially disposed.
The implementation method comprises the following steps:
step one: the lens 1 images a real image of a scene image on a blazed grating 2 at a back focal plane, the scene image light signal is dispersed after passing through the blazed grating 2, the spectrum signal after the scene image dispersion is screened by a band-pass filter 3, and the spectrum wave band is kept as lambda 1 To lambda n The aliased spectral signal is refocused on the lenticular array 5 through the achromatic relay lens 4;
step two: different emergent angles exist in different wavelength spectrum signals of the scene image at the cylindrical lens array 5, light rays are not affected when passing through the tangential direction of the cylindrical lens 5, and the light rays can have numerical aperture NA for incident light when passing through the normal direction of the cylindrical lens 5 o Will be magnified and overlay the pixel array of the camera 6. In the sub-pixel area 7 covered by each cylindrical mirror, the width of the sub-pixel strip 7 along the normal direction of the cylindrical mirror is n pixels, scene image spectrum signals are spread and distributed along the normal direction of the cylindrical mirror, and no dispersion exists along the tangential scene image of the cylindrical mirror;
step three: the spectral band is lambda 1 To lambda n Evenly divided into n parts, namely lambda 1 ，λ 2 ，…，λ n Will beThe ith row in the sub-pixel strip 7 correspondingly covered by the cylindrical lens in the pixel area is combined together to obtain a picture P i . At this time, picture P i At spectral wavelength lambda for a scene i Imaging under light irradiation, where i=1, 2, …, n.
Further, the scene image light signal projected onto the lenticular array 5 must match as closely as possible the numerical aperture of the lenticular array 5. The method comprises the following steps:
let the numerical aperture of the light beam of the lens 1 at the rear focusing surface 2 be NA o The magnification of achromatic lens group 4 is N, then the grating is at λ 1 To lambda n The amplification factor M of the numerical aperture of the band spectrum signal is as follows:
wherein,α＝arcsin(NA o ) And/2, d=a+b, wherein a is the width of the light-transmitting slit of the grating scribe line, and b is the width of the light-impermeable scribe part.
Let the F number of the cylindrical lens array 5 be F # Then
The above equal sign holds as the optimal solution.
Claims (2)
1. The realization method of the spectrum imaging device is characterized in that the spectrum imaging device comprises a lens (1), a blazed grating (2), a band-pass filter (3), an achromatic relay lens group (4), a cylindrical lens array (5) and a camera (6), wherein each cylindrical lens in the camera corresponds to a group of sub-pixel strips (7);
under natural light, scene images observed by a lens (1) are converged on a back focal plane of the lens (1), a blazed grating (2) is arranged at the back focal plane, the blazed grating (2) disperses the scene images recorded by the lens (1) and relays the scene images to one side of a substrate of a cylindrical lens array (5) through an achromatic relay lens group (4), at the moment, the scene images at the blazed grating (2) and the cylindrical lens array (5) are not dispersed, and the scene images are dispersed in the process of being transmitted from the blazed grating (2) to the cylindrical lens array (5); the pixel array of the camera (6) is positioned at the back focal plane of the cylindrical lens array (5), and light signals with different wavelengths of scene images converged on the cylindrical lens array (5) have different emergence angles, so that the light signals with different wavelengths of the scene images passing through the cylindrical lens array (5) can be dispersed along the normal direction of the cylindrical lens and spread over the pixel array of the camera (6), and the tangential scene images of the cylindrical lens are not dispersed;
the implementation method of the spectrum imaging device comprises the following steps:
step one: the lens (1) images a real image of a scene image on the blazed grating (2) at the back focal plane, the scene image optical signal is dispersed after passing through the blazed grating (2), the spectrum signal after the scene image dispersion is screened by the band-pass filter (3), and the spectrum wave band in the spectrum signal is kept as lambda 1 To lambda n The aliased spectrum signal is refocused on the cylindrical lens array (5) through the achromatic relay lens (4);
step two: different emergent angles exist in different wavelength spectrum signals of a scene image at the position of the cylindrical lens array (5), light rays are not affected when passing through the tangential direction of the cylindrical lens (5), and the light rays can have numerical aperture NA (NA) on incident light when passing through the normal direction of the cylindrical lens (5) o Will be magnified, covering over the pixel array of the camera (6); in a sub-pixel area (7) covered by each cylindrical mirror, the width of a sub-pixel strip (7) along the normal direction of the cylindrical mirror is n pixels, scene image spectrum signals are unfolded and distributed along the normal direction of the cylindrical mirror, and no dispersion exists along the tangential scene images of the cylindrical mirror;
step three: the spectral band is lambda 1 To lambda n Evenly divided into n parts, namely lambda 1 ，λ 2 ，…，λ n Combining the ith row in the sub-pixel strip (7) correspondingly covered by the cylindrical lens in the pixel area to obtain a picture P i The method comprises the steps of carrying out a first treatment on the surface of the At this time, picture P i At spectral wavelength lambda for a scene i Imaging under light irradiation, where i=1, 2, …, n.
2. A method of realising a spectral imaging device according to claim 1, wherein the light signal of the scene image projected onto the lenticular array (5) must match as much as possible the numerical aperture of the lenticular array (5); the method comprises the following steps:
let NA be the numerical aperture of the light beam of the lens (1) at the rear focusing surface (2) o The achromatic lens group (4) has a magnification of N, and the grating is arranged at lambda 1 To lambda n The amplification factor M of the numerical aperture of the band spectrum signal is as follows:
wherein,α＝arcsin(NA o ) 2, d=a+b, wherein a is the width of the light-transmitting slit of the grating inscription line, and b is the width of the light-impermeable inscription part;
setting the F number of the cylindrical lens array (5) as F # Then
The above equal sign holds as the optimal solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111340651.1A CN114112043B (en) | 2021-11-12 | 2021-11-12 | Spectrum imaging device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111340651.1A CN114112043B (en) | 2021-11-12 | 2021-11-12 | Spectrum imaging device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114112043A CN114112043A (en) | 2022-03-01 |
| CN114112043B true CN114112043B (en) | 2023-11-07 |
Family
ID=80379298
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111340651.1A Active CN114112043B (en) | 2021-11-12 | 2021-11-12 | Spectrum imaging device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114112043B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107436194A (en) * | 2017-06-22 | 2017-12-05 | 北京理工大学 | A kind of high light flux real time spectrum imaging device |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150153156A1 (en) * | 2013-12-03 | 2015-06-04 | Mvm Electronics, Inc. | High spatial and spectral resolution snapshot imaging spectrometers using oblique dispersion |
| US10018560B2 (en) * | 2016-02-02 | 2018-07-10 | Kla-Tencor Corporation | System and method for hyperspectral imaging metrology |
-
2021
- 2021-11-12 CN CN202111340651.1A patent/CN114112043B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107436194A (en) * | 2017-06-22 | 2017-12-05 | 北京理工大学 | A kind of high light flux real time spectrum imaging device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114112043A (en) | 2022-03-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU731476C (en) | Multi-spectral two-dimensional imaging spectrometer | |
| US5982497A (en) | Multi-spectral two-dimensional imaging spectrometer | |
| US8351031B2 (en) | Single-shot spectral imager | |
| US7636158B1 (en) | Optimal coupling of high performance line imaging spectrometer to imaging system | |
| WO2019027744A1 (en) | Overlay metrology using multiple parameter configurations | |
| US11409092B2 (en) | Parallel multi-region imaging device | |
| JP6290416B2 (en) | Apparatus and method for performing inline lens-free digital holography of an object | |
| CN112097905B (en) | Spectral microscopic imaging device | |
| US4541688A (en) | Optical beam splitters | |
| JP2009527953A (en) | Optical multiplexed imaging system and operation method | |
| CN107462329B (en) | Multispectral camera, multispectral imaging device and control method | |
| CN113568156B (en) | Spectral microscopic imaging device and implementation method | |
| CN112082649A (en) | Video hyperspectral imager based on array slit scanning | |
| US20130250088A1 (en) | Multi-color confocal microscope and imaging methods | |
| CN114112043B (en) | Spectrum imaging device | |
| CN112067128A (en) | High-speed static dispersion imaging spectrum device and using method thereof | |
| CN113514944B (en) | Quick spectral microscopic imaging device | |
| US20080180791A1 (en) | Photosensor-chip, laser-microscope comprising a photosensor-chip and method for reading a photosensor-chip | |
| US10969523B1 (en) | Apparatus and method to obtain intrinsic still and video images without the use of filters or dichroic mirrors | |
| CN113534434A (en) | Spectral microscopic imaging device based on LED array and implementation method thereof | |
| US3664741A (en) | Method and devices for the chromatic analysis of an object | |
| JP2012138652A (en) | Tunable filter camera and scanner | |
| CN113330299B (en) | Imaging reflectometer | |
| US20230247276A1 (en) | Re-imaging microscopy with micro-camera array | |
| WO2023211772A1 (en) | Lens array based imaging system with improved field of view |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |